pycom03g06650

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000257
Physical Location & Seq
Reverse (-)
5206893 .. 5210429
3537 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06650.9

Sequence Viewer

Length: 2199 bp
ATGGCAAAGAAAGGGGCTGTTGCGTTTTATTCTGTTTTCTCCATTTTTATTCTGAGCATGAGCTTATTATGCAAAGCCACCGATGAAGATAGAAAGGTCTATATTGTGTACATGGGGTCCCTTCCTGATGGAGATCCGGCGTACTCACCGTTGGCTCACCACCTTAGTATTCTACACAGTGTTGTTCAGGATGGTTCTCCAGAAAATTTATTGGTAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAGACTGACCCACCAGGAAAGTGAGAAAATTGCGAACATGAAGGAAGTAGTCTCTGTCTTTCCAAGCCGAATGTTCCAACGTCAAACAACAAGATCTTGGGACTTTATGGGCTTAACTGAAATGGCCAGTCGAAATTCTTCCCAACTTCAGAGTGATGTTATTGTTGCTTTGTTTGACTCTGGAGTCTGGCCTGAATCTGAGAGCTTTAAAGATGAAGGTCTAGGTCCTGCTCCAAAGCATTGGAAAGGTGTTTGTGACGGCGGAAAAAATTTCACTTGCAATAACAAGCTCATTGGAGCTCGATATTACACATCAGAGACTGCAAGAGATGATATAGGTCATGGAACTCACACATCGTCCACAGTGGCAGGCAGTGCTGTTAAGGATGTAACCTTTTATGGACTAGCAAACGGCACTGCAAGAGGAGGCGTTCCTGCTGCGAGACTTGTTGCATATAAAGTCTGCGATCCATGCAGAGATCACAATATACTGGCTGCTTACGATGATGCTATTGCTGATGGAGTCAACATTATTTCTATTTCACTTGTATCCGACTGGCTAACTTCGATTCATCAGGACGTTGTTGCTATTGGTGCTTTTCATGCAATGAAAAAAGGGATACTGGTTTCGCAGGCTGGAGGCAACTTGGGCCCGTTTGTTGCTTCTGTGACCAGTGTAGCACCATGGATATTGACAGTTGCAGCAAGTAGCATTGATCGGCTTTTTGTGGACAAGGTTGTTATTGGCAATGGAAAGACAGTAGCTGGGGATGACTGGATTAACATGTTCACATTAAATGGAACAAGTTTTCCTCTGATACACGGAAAAGATGCTGCAACGAAAAATTGCACCGAAGATGATGCTAGACAATGTGAAGAGGGTTGCCTGGTAGATGATTTAGTTAAGGGAAAGATAGTTGTATGCGATAACGTCACCGGAGTTATAGGGGCAAACACGGCTGGTGCGCTCGGTTCAATTTTATTTAATCCATTTGAAGTTAATATTGCCGTTATTCCCACATTAGCCTCAGCACCTTTAAAGAAAGAAGAGTATAATGTTGTCAGATCCTACATGAATTCCACCAGAGATCCTCAAGGAAACATACTTAAAAGTGAAGTCACAAAAGATCCTGCTGCACCTACAGTCCTGTTCTTCTCATCACGTGGACCTAATGGAATTTTACCCGATTTAGTCAAGCCGGATATAAGTGCCCCGGGGTATGAAATTTTGGCTGCGTTTCCACCTGTTCTTCCTATCACCGACTTTCCGGATGATAAGAGGCGTGCAAAATACAATATAGCATCTGGTACCTCCATGGCTTGCCCCCACGCTGCAGGGGTAGCCGCATATGTTAAAGCATTCCACCCTGATTGGTCTGCTGCAGCTATCAAATCATCTATTATGACAACTGCTTGGCCCATGAACGATACCGACAACGATGTTTCAAATGGTGCATTTGCTTATGGATCCGGACATATAAATCCTGTAAATGCTATTAATCCGGGGCTAGTCTATGATGCTTCTGAAGAAGATTACATAAAGCTGCTGTGCACAATCTATGATGAAGGCACGGTTAGAAAAATATCTGGAGATAACAGCACATGCCCAACTGCTAGAGGATTTGTAAAGGATCACAATTACCCTTCAATGGGAGCTCAAGTTGAAGTATTGAAACCTTTTTCGATTAATTTTCACAGAAGAGTTAAAAACGTTGGTCTCGCAAACTCCACTTACAAGGCCAAAATCCTCTCGGATTCGAAAGTGGATATCAAAGTAGTGCCTGAGGTTCTGTCATTCGACTCCTTGAATGAGGAGAAGGATTTTGATGTAACCGTTGTTGGAAGCGGTGTAAGAGATCAATCACAAGTATCTGGATCACTGGTTTGGTCAGATGGTATTTACAGTGTTAGAAGTCCAATTGTTGTTTATGCATATGTTCACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

733

Amino Acids

78.83

Weight (kDa)

5.88

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
Acc65I GGTACC 1 cut(s) 1559
AccB1I GGYRCC 1 cut(s) 1559
AccIII TCCGGA 2 cut(s) 1519, 1721
AciI CCGC 3 cut(s) 513, 1596, 2097
AclI AACGTT 1 cut(s) 1962
AclWI GGATC 9 cut(s) 128, 713, 1311, 1334, 1373, 1713, 1726, 1890, 2134
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 6 cut(s) 205, 385, 520, 1327, 1428, 1476
AcuI CTGAAG 2 cut(s) 383, 1797
AcvI CACGTG 1 cut(s) 1415
AfaI GTAC 3 cut(s) 110, 143, 1561
AfiI CCNNNNNNNGG 2 cut(s) 1900, 1901
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 8 cut(s) 238, 1227, 1247, 1698, 1899, 1916, 1924, 2059
AjnI CCWGG 2 cut(s) 264, 1137
AloI GAACNNNNNNTCC 2 cut(s) 1045, 1077
AluBI AGCT 9 cut(s) 63, 456, 541, 551, 1016, 1637, 1795, 1907, 2196
AluI AGCT 9 cut(s) 63, 456, 541, 551, 1016, 1637, 1795, 1907, 2196
Alw21I GWGCWC 3 cut(s) 553, 1805, 1909
Alw26I GTCTC 4 cut(s) 307, 563, 688, 1973
Alw44I GTGCAC 1 cut(s) 1801
AlwI GGATC 9 cut(s) 128, 713, 1311, 1334, 1373, 1713, 1726, 1890, 2134
AlwNI CAGNNNCTG 2 cut(s) 572, 1016
Ama87I CYCGRG 1 cut(s) 1465
Aor13HI TCCGGA 2 cut(s) 1519, 1721
AoxI GGCC 5 cut(s) 375, 440, 901, 1667, 1989
ApaI GGGCCC 1 cut(s) 905
ApaLI GTGCAC 1 cut(s) 1801
ApoI RAATTY 6 cut(s) 205, 385, 520, 1327, 1428, 1476
AseI ATTAAT 2 cut(s) 1749, 1938
Asp700I GAANNNNTTC 1 cut(s) 388
Asp718I GGTACC 1 cut(s) 1559
AspLEI GCGC 1 cut(s) 1219
AspS9I GGNCC 6 cut(s) 117, 476, 901, 902, 1418, 1668
AsuC2I CCSGG 3 cut(s) 1466, 1467, 1755
AsuHPI GGTGA 4 cut(s) 138, 149, 1177, 1501
AsuII TTCGAA 1 cut(s) 2009
AvaI CYCGRG 1 cut(s) 1465
AvaII GGWCC 3 cut(s) 117, 476, 1418
AxyI CCTNAGG 1 cut(s) 2034
BaeGI GKGCMC 3 cut(s) 905, 1465, 1805
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 1 cut(s) 1718
BanI GGYRCC 1 cut(s) 1559
BanII GRGCYC 3 cut(s) 553, 905, 1909
BbrPI CACGTG 1 cut(s) 1415
Bbv12I GWGCWC 3 cut(s) 553, 1805, 1909
BbvCI CCTCAGC 1 cut(s) 1279
BccI CCATC 4 cut(s) 122, 185, 764, 2138
BceAI ACGGC 4 cut(s) 526, 679, 1224, 1244
BcgI CGANNNNNNTGC 2 cut(s) 1094, 1128
BciT130I CCWGG 2 cut(s) 266, 1139
BciVI GTATCC 2 cut(s) 811, 864
BcnI CCSGG 3 cut(s) 1466, 1467, 1755
BcoDI GTCTC 4 cut(s) 307, 563, 688, 1973
BfaI CTAG 6 cut(s) 473, 656, 1116, 1760, 1866, 2197
BfmI CTRYAG 3 cut(s) 1392, 1584, 1632
BfuI GTATCC 2 cut(s) 811, 864
BglII AGATCT 1 cut(s) 344
Bme1390I CCNGG 5 cut(s) 266, 1139, 1466, 1467, 1755
Bme18I GGWCC 3 cut(s) 117, 476, 1418
BmeT110I CYCGRG 1 cut(s) 1465
BmgT120I GGNCC 6 cut(s) 117, 476, 901, 902, 1418, 1668
BmiI GGNNCC 5 cut(s) 118, 119, 903, 1561, 1720
BmrFI CCNGG 5 cut(s) 266, 1139, 1466, 1467, 1755
BmsI GCATC 5 cut(s) 748, 1072, 1102, 1562, 1759
BpmI CTGGAG 4 cut(s) 183, 453, 909, 1860
Bpu10I CCTNAGC 1 cut(s) 1279
Bpu14I TTCGAA 1 cut(s) 2009
BpuEI CTTGAG 2 cut(s) 1329, 1893
BpuMI CCSGG 3 cut(s) 1466, 1467, 1755
BsaAI YACGTR 1 cut(s) 1415
BsaBI GATNNNNATC 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 1973
BsaJI CCNNGG 6 cut(s) 935, 1464, 1465, 1466, 1566, 1754
BsaWI WCCGGW 3 cut(s) 1187, 1519, 1721
BsaXI ACNNNNNCTCC 2 cut(s) 1896, 1926
Bsc4I CCNNNNNNNGG 2 cut(s) 1900, 1901
Bse1I ACTGG 7 cut(s) 378, 747, 812, 879, 924, 1031, 2136
Bse21I CCTNAGG 1 cut(s) 2034
Bse3DI GCAATG 2 cut(s) 864, 1006
Bse8I GATNNNNATC 1 cut(s) 132
BseAI TCCGGA 2 cut(s) 1519, 1721
BseBI CCWGG 2 cut(s) 266, 1139
BseDI CCNNGG 6 cut(s) 935, 1464, 1465, 1466, 1566, 1754
BseGI GGATG 4 cut(s) 196, 643, 1027, 1528
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 1900, 1901
BseMI GCAATG 2 cut(s) 864, 1006
BseMII CTCAG 4 cut(s) 44, 441, 1293, 2025
BseNI ACTGG 7 cut(s) 378, 747, 812, 879, 924, 1031, 2136
BseRI GAGGAG 2 cut(s) 690, 2078
BseSI GKGCMC 3 cut(s) 905, 1465, 1805
BseYI CCCAGC 1 cut(s) 1016
BsgI GTGCAG 1 cut(s) 1371
BshFI GGCC 5 cut(s) 377, 442, 903, 1669, 1991
BshNI GGYRCC 1 cut(s) 1559
BsiHKAI GWGCWC 3 cut(s) 553, 1805, 1909
BsiHKCI CYCGRG 1 cut(s) 1465
BsiSI CCGG 7 cut(s) 137, 1188, 1451, 1466, 1520, 1722, 1754
BslFI GGGAC 2 cut(s) 103, 365
BslI CCNNNNNNNGG 2 cut(s) 1900, 1901
BsmAI GTCTC 4 cut(s) 307, 563, 688, 1973
BsmFI GGGAC 2 cut(s) 103, 365
BsmI GAATGC 1 cut(s) 1610
BsnI GGCC 5 cut(s) 377, 442, 903, 1669, 1991
Bso31I GGTCTC 1 cut(s) 1973
BsoBI CYCGRG 1 cut(s) 1465
Bsp119I TTCGAA 1 cut(s) 2009
Bsp120I GGGCCC 1 cut(s) 901
Bsp1286I GDGCHC 5 cut(s) 553, 905, 1465, 1805, 1909
Bsp13I TCCGGA 2 cut(s) 1519, 1721
Bsp1407I TGTACA 1 cut(s) 108
Bsp19I CCATGG 2 cut(s) 935, 1566
BspACI CCGC 3 cut(s) 513, 1596, 2097
BspANI GGCC 5 cut(s) 377, 442, 903, 1669, 1991
BspCNI CTCAG 4 cut(s) 45, 442, 1292, 2026
BspEI TCCGGA 2 cut(s) 1519, 1721
BspLI GGNNCC 5 cut(s) 118, 119, 903, 1561, 1720
BspMAI CTGCAG 2 cut(s) 1588, 1636
BspPI GGATC 9 cut(s) 128, 713, 1311, 1334, 1373, 1713, 1726, 1890, 2134
BspT104I TTCGAA 1 cut(s) 2009
BspT107I GGYRCC 1 cut(s) 1559
BspTNI GGTCTC 1 cut(s) 1973
BsrDI GCAATG 2 cut(s) 864, 1006
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 7 cut(s) 378, 747, 812, 879, 924, 1031, 2136
BssECI CCNNGG 6 cut(s) 935, 1464, 1465, 1466, 1566, 1754
BssT1I CCWWGG 2 cut(s) 935, 1566
Bst2UI CCWGG 2 cut(s) 266, 1139
Bst4CI ACNGT 9 cut(s) 150, 179, 616, 949, 1012, 1396, 1825, 2086, 2156
Bst6I CTCTTC 3 cut(s) 1122, 1293, 1945
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 1415
BstBI TTCGAA 1 cut(s) 2009
BstC8I GCNNGC 4 cut(s) 622, 885, 1536, 1573
BstDEI CTNAG 5 cut(s) 53, 164, 450, 1279, 2034
BstDSI CCRYGG 2 cut(s) 935, 1566
BstF5I GGATG 4 cut(s) 196, 643, 1027, 1528
BstHHI GCGC 1 cut(s) 1219
BstMAI GTCTC 4 cut(s) 307, 563, 688, 1973
BstMWI GCNNNNNNNGC 8 cut(s) 69, 626, 723, 845, 854, 900, 1208, 1592
BstNI CCWGG 2 cut(s) 266, 1139
BstNSI RCATGY 2 cut(s) 1039, 1857
BstSCI CCNGG 5 cut(s) 264, 1137, 1464, 1465, 1753
BstSFI CTRYAG 3 cut(s) 1392, 1584, 1632
BstSLI GKGCMC 3 cut(s) 905, 1465, 1805
BstX2I RGATCY 6 cut(s) 133, 344, 1316, 1339, 1378, 1718
BstXI CCANNNNNNTGG 1 cut(s) 492
BstYI RGATCY 6 cut(s) 133, 344, 1316, 1339, 1378, 1718
Bsu36I CCTNAGG 1 cut(s) 2034
BsuI GTATCC 2 cut(s) 811, 864
BsuRI GGCC 5 cut(s) 377, 442, 903, 1669, 1991
BtgI CCRYGG 2 cut(s) 935, 1566
BtsCI GGATG 4 cut(s) 196, 643, 1027, 1528
BtsI GCAGTG 2 cut(s) 631, 666
BtsIMutI CAGTG 7 cut(s) 184, 621, 631, 666, 931, 2129, 2161
Cac8I GCNNGC 4 cut(s) 622, 885, 1536, 1573
CaiI CAGNNNCTG 2 cut(s) 572, 1016
CfoI GCGC 1 cut(s) 1219
Cfr13I GGNCC 6 cut(s) 117, 476, 901, 902, 1418, 1668
Cfr9I CCCGGG 1 cut(s) 1465
Csp6I GTAC 3 cut(s) 109, 142, 1560
CspCI CAANNNNNGTGG 2 cut(s) 1604, 1639
CviQI GTAC 3 cut(s) 109, 142, 1560
DdeI CTNAG 5 cut(s) 53, 164, 450, 1279, 2034
DraI TTTAAA 2 cut(s) 460, 1290
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 3 cut(s) 1122, 1293, 1945
EarI CTCTTC 3 cut(s) 1122, 1293, 1945
EciI GGCGGA 1 cut(s) 528
Ecl136II GAGCTC 2 cut(s) 551, 1907
Eco130I CCWWGG 2 cut(s) 935, 1566
Eco24I GRGCYC 3 cut(s) 553, 905, 1909
Eco31I GGTCTC 1 cut(s) 1973
Eco32I GATATC 1 cut(s) 2020
Eco47I GGWCC 3 cut(s) 117, 476, 1418
Eco53kI GAGCTC 2 cut(s) 551, 1907
Eco57I CTGAAG 2 cut(s) 383, 1797
Eco72I CACGTG 1 cut(s) 1415
Eco81I CCTNAGG 1 cut(s) 2034
Eco88I CYCGRG 1 cut(s) 1465
EcoICRI GAGCTC 2 cut(s) 551, 1907
EcoO109I RGGNCCY 2 cut(s) 117, 476
EcoRI GAATTC 1 cut(s) 1327
EcoRII CCWGG 2 cut(s) 264, 1137
EcoRV GATATC 1 cut(s) 2020
EcoT14I CCWWGG 2 cut(s) 935, 1566
EcoT22I ATGCAT 1 cut(s) 2185
EcoT38I GRGCYC 3 cut(s) 553, 905, 1909
ErhI CCWWGG 2 cut(s) 935, 1566
FaqI GGGAC 2 cut(s) 103, 365
FauNDI CATATG 2 cut(s) 1600, 2185
FokI GGATG 4 cut(s) 203, 650, 1034, 1535
FriOI GRGCYC 3 cut(s) 553, 905, 1909
FspBI CTAG 6 cut(s) 473, 656, 1116, 1760, 1866, 2197
GlaI GCGC 1 cut(s) 1218
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 4 cut(s) 183, 453, 909, 1860
HaeIII GGCC 5 cut(s) 377, 442, 903, 1669, 1991
HapII CCGG 7 cut(s) 137, 1188, 1451, 1466, 1520, 1722, 1754
HhaI GCGC 1 cut(s) 1219
Hin6I GCGC 1 cut(s) 1217
HinP1I GCGC 1 cut(s) 1217
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 7 cut(s) 428, 435, 446, 774, 820, 2006, 2051
HpaII CCGG 7 cut(s) 137, 1188, 1451, 1466, 1520, 1722, 1754
HphI GGTGA 4 cut(s) 138, 149, 1177, 1501
Hpy166II GTNNAC 8 cut(s) 109, 612, 778, 982, 1041, 1418, 1803, 2191
Hpy188III TCNNGA 9 cut(s) 125, 188, 200, 432, 827, 1520, 1722, 1839, 2124
Hpy8I GTNNAC 8 cut(s) 109, 612, 778, 982, 1041, 1418, 1803, 2191
HpyAV CCTTC 6 cut(s) 131, 286, 461, 1811, 1905, 2062
HpyCH4III ACNGT 9 cut(s) 150, 179, 616, 949, 1012, 1396, 1825, 2086, 2156
HpyCH4IV ACGT 5 cut(s) 331, 831, 1182, 1414, 1962
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 626, 723, 845, 854, 900, 1208, 1592
HpyF3I CTNAG 5 cut(s) 53, 164, 450, 1279, 2034
HpySE526I ACGT 5 cut(s) 331, 831, 1182, 1414, 1962
HspAI GCGC 1 cut(s) 1217
KflI GGGWCCC 1 cut(s) 117
Kpn2I TCCGGA 2 cut(s) 1519, 1721
KpnI GGTACC 1 cut(s) 1563
LmnI GCTCC 3 cut(s) 487, 548, 1904
LweI GCATC 5 cut(s) 748, 1072, 1102, 1562, 1759
MaeI CTAG 6 cut(s) 473, 656, 1116, 1760, 1866, 2197
MaeII ACGT 5 cut(s) 331, 831, 1182, 1414, 1962
MaeIII GTNAC 7 cut(s) 221, 506, 640, 919, 1183, 1369, 2080
MfeI CAATTG 1 cut(s) 2169
MflI RGATCY 6 cut(s) 133, 344, 1316, 1339, 1378, 1718
MhlI GDGCHC 5 cut(s) 553, 905, 1465, 1805, 1909
MlsI TGGCCA 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 4 cut(s) 422, 444, 783, 2045
MmeI TCCRAC 3 cut(s) 352, 828, 2071
Mox20I TGGCCA 1 cut(s) 377
Mph1103I ATGCAT 1 cut(s) 2185
MroI TCCGGA 2 cut(s) 1519, 1721
MroXI GAANNNNTTC 1 cut(s) 388
MscI TGGCCA 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 377
MspI CCGG 7 cut(s) 137, 1188, 1451, 1466, 1520, 1722, 1754
MspR9I CCNGG 5 cut(s) 266, 1139, 1466, 1467, 1755
MunI CAATTG 1 cut(s) 2169
Mva1269I GAATGC 1 cut(s) 1610
MvaI CCWGG 2 cut(s) 266, 1139
MwoI GCNNNNNNNGC 8 cut(s) 69, 626, 723, 845, 854, 900, 1208, 1592
NciI CCSGG 3 cut(s) 1466, 1467, 1755
NcoI CCATGG 2 cut(s) 935, 1566
NdeI CATATG 2 cut(s) 1600, 2185
NlaIV GGNNCC 5 cut(s) 118, 119, 903, 1561, 1720
NmuCI GTSAC 4 cut(s) 506, 919, 1183, 1369
NsiI ATGCAT 1 cut(s) 2185
NspI RCATGY 2 cut(s) 1039, 1857
NspV TTCGAA 1 cut(s) 2009
PciI ACATGT 1 cut(s) 1035
PctI GAATGC 1 cut(s) 1610
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 3 cut(s) 446, 820, 2006
PleI GAGTC 4 cut(s) 422, 443, 782, 2045
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
PpsI GAGTC 4 cut(s) 422, 443, 782, 2045
Ppu21I YACGTR 1 cut(s) 1415
PpuMI RGGWCCY 2 cut(s) 117, 476
PscI ACATGT 1 cut(s) 1035
PshBI ATTAAT 2 cut(s) 1749, 1938
Psp124BI GAGCTC 2 cut(s) 553, 1909
Psp1406I AACGTT 1 cut(s) 1962
Psp5II RGGWCCY 2 cut(s) 117, 476
Psp6I CCWGG 2 cut(s) 264, 1137
PspCI CACGTG 1 cut(s) 1415
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 2 cut(s) 264, 1137
PspN4I GGNNCC 5 cut(s) 118, 119, 903, 1561, 1720
PspOMI GGGCCC 1 cut(s) 901
PspPI GGNCC 6 cut(s) 117, 476, 901, 902, 1418, 1668
PspPPI RGGWCCY 2 cut(s) 117, 476
PstI CTGCAG 2 cut(s) 1588, 1636
PstNI CAGNNNCTG 2 cut(s) 572, 1016
PsuI RGATCY 6 cut(s) 133, 344, 1316, 1339, 1378, 1718
RsaI GTAC 3 cut(s) 110, 143, 1561
RsaNI GTAC 3 cut(s) 109, 142, 1560
SacI GAGCTC 2 cut(s) 553, 1909
Sau96I GGNCC 6 cut(s) 117, 476, 901, 902, 1418, 1668
SchI GAGTC 4 cut(s) 422, 444, 783, 2045
ScrFI CCNGG 5 cut(s) 266, 1139, 1466, 1467, 1755
SduI GDGCHC 5 cut(s) 553, 905, 1465, 1805, 1909
SfaNI GCATC 5 cut(s) 748, 1072, 1102, 1562, 1759
SfcI CTRYAG 3 cut(s) 1392, 1584, 1632
SfuI TTCGAA 1 cut(s) 2009
SinI GGWCC 3 cut(s) 117, 476, 1418
SmaI CCCGGG 1 cut(s) 1467
SmlI CTYRAG 2 cut(s) 1344, 1908
SmoI CTYRAG 2 cut(s) 1344, 1908
SsiI CCGC 3 cut(s) 513, 1596, 2097
SspI AATATT 1 cut(s) 1255
SspMI CTAG 6 cut(s) 473, 656, 1116, 1760, 1866, 2197
SstI GAGCTC 2 cut(s) 553, 1909
StyD4I CCNGG 5 cut(s) 264, 1137, 1464, 1465, 1753
StyI CCWWGG 2 cut(s) 935, 1566
TaaI ACNGT 9 cut(s) 150, 179, 616, 949, 1012, 1396, 1825, 2086, 2156
TaiI ACGT 5 cut(s) 334, 834, 1185, 1417, 1965
TaqI TCGA 6 cut(s) 382, 553, 818, 1934, 2009, 2049
TatI WGTACW 1 cut(s) 108
TauI GCSGC 1 cut(s) 1598
TfiI GAWTC 3 cut(s) 446, 820, 2006
TscAI CASTG 7 cut(s) 184, 621, 631, 673, 931, 2136, 2161
TseFI GTSAC 4 cut(s) 506, 919, 1183, 1369
Tsp45I GTSAC 4 cut(s) 506, 919, 1183, 1369
TspGWI ACGGA 1 cut(s) 1089
TspMI CCCGGG 1 cut(s) 1465
TspRI CASTG 7 cut(s) 184, 621, 631, 673, 931, 2136, 2161
VneI GTGCAC 1 cut(s) 1801
VpaK11BI GGWCC 3 cut(s) 117, 476, 1418
VspI ATTAAT 2 cut(s) 1749, 1938
XapI RAATTY 6 cut(s) 205, 385, 520, 1327, 1428, 1476
XceI RCATGY 2 cut(s) 1039, 1857
XmaI CCCGGG 1 cut(s) 1465
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 6 cut(s) 473, 656, 1116, 1760, 1866, 2197
Zsp2I ATGCAT 1 cut(s) 2185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.