pycom02g24510

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000257
Physical Location & Seq
Forward (+)
22661130 .. 22662185
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24510.1

Sequence Viewer

Length: 1056 bp
ATGAAGGGAGTTGTCTCTGTGTTTCCGCGCACAATTTACCGCCTTCAAGCAACAAGATCTTGGGACTTCATCGAACTCAATGAGACGATCAAACGAAACCCTACTGTCCAAAGTGATGTTGTTATTGGTGTGATAGACGGTGGAATCTGGCCTGAATCGGAGAGCTTTAAAGACGAAGGTTTTGGTCCAGCTTCCAAGAAGTGGAAAGGTGTTTGCAAAGGTGGCAATAATTTCACTTGCAACAACAAGCTCACTGGAGCTCGGTCTTATATTGATTCTACGGCTAGAGATAAGATTGGTCATGGGACTCATACTGCTTCAACCGCAGCAGGTAACCCCGTAAAGGGTGTGAGCTTTTATGGACTAGGGCAAGGGACTGCAAGAGGAGGAGTTCCCTCTGCGAGAATTGCTGCATATAAAGTTTGCGGTTCTCAAGGGTGCACTTCAGATGGGATCTTGGCTGCTTTTGATGACGCCATTGGCGACGGAGTTGACATAATCACAATTTCAATTGGAGGCGACTATGGAGTGCCTTTTGAGAACGATCCTGTTGCAATCTGTGCTTTTCATGCAATGGAGAAAGGGATACTTACCTCAAACTCTGCAGGCAATAACGGTCCTGTAGAGACTTCTATAGCAAGTGCAGCACCATGGATTCTTACAGTTGCAGCAAGTAGCACGGATCGTCGGATAATTGACAAGGTTGTTCTTGGAAATGGAGGGACGCTTGTCGGAAGTTCAGTGAATGCTTTCAAATTAAATGGAGCAAGTTTTCCTTTGATAGAAGGAAAAAATGTTTCAAGTCAATGCTGGGATTGTAACGTAGGCTGCCTGGACCCTAATTTCGTTAGGGGAAAGATTTTGGTATGTCTTCGTTCTGTTGCCAATACTGTGGCTTATGAAGCCGGTGCCGCAGGTGCAATTACGATCAGTTCCAAACCGAACCCGATGTTTCTTTCATTGTCCCGCTACCTGCAGCAACTATTAGCAGAAAAGATTATGATGTGGTGCAATCCTACCTCAACTCCACAAACAAACCTCAAGCGAAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

352

Amino Acids

37.05

Weight (kDa)

8.75

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 38 - 235 1.8e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 905
Acc36I ACCTGC 3 cut(s) 320, 905, 981
AccB1I GGYRCC 1 cut(s) 908
AccB7I CCANNNNNTGG 1 cut(s) 201
AccII CGCG 1 cut(s) 28
AciI CCGC 6 cut(s) 26, 40, 324, 426, 912, 967
AclWI GGATC 3 cut(s) 461, 539, 690
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 474
AfiI CCNNNNNNNGG 3 cut(s) 201, 343, 344
AgsI TTSAA 5 cut(s) 47, 321, 510, 754, 801
AjnI CCWGG 1 cut(s) 831
AluBI AGCT 5 cut(s) 165, 191, 250, 260, 354
AluI AGCT 5 cut(s) 165, 191, 250, 260, 354
Alw21I GWGCWC 2 cut(s) 262, 443
Alw26I GTCTC 3 cut(s) 19, 77, 620
Alw44I GTGCAC 1 cut(s) 439
AlwI GGATC 3 cut(s) 461, 539, 690
AoxI GGCC 1 cut(s) 149
ApaLI GTGCAC 1 cut(s) 439
ApeKI GCWGC 7 cut(s) 326, 410, 461, 644, 668, 828, 976
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 827, 859
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 3 cut(s) 185, 617, 835
AvaII GGWCC 3 cut(s) 185, 617, 835
BaeGI GKGCMC 1 cut(s) 443
BanI GGYRCC 1 cut(s) 908
BanII GRGCYC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 2 cut(s) 262, 443
BbvI GCAGC 7 cut(s) 338, 397, 448, 656, 680, 815, 988
BccI CCATC 1 cut(s) 443
BceAI ACGGC 1 cut(s) 297
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 1 cut(s) 833
BciVI GTATCC 1 cut(s) 579
BcoDI GTCTC 3 cut(s) 19, 77, 620
BfaI CTAG 2 cut(s) 285, 365
BfmI CTRYAG 4 cut(s) 603, 621, 633, 974
BfuAI ACCTGC 3 cut(s) 320, 905, 981
BfuI GTATCC 1 cut(s) 579
BglII AGATCT 1 cut(s) 56
BisI GCNGC 8 cut(s) 327, 411, 462, 645, 669, 829, 912, 977
BlsI GCNGC 8 cut(s) 328, 412, 463, 646, 670, 830, 913, 978
Bme1390I CCNGG 1 cut(s) 833
Bme18I GGWCC 3 cut(s) 185, 617, 835
BmgT120I GGNCC 3 cut(s) 185, 617, 835
BmiI GGNNCC 2 cut(s) 837, 910
BmrFI CCNGG 1 cut(s) 833
BoxI GACNNNNGTC 1 cut(s) 728
BpiI GAAGAC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 417, 1025
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 343, 344
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 1 cut(s) 650
BseLI CCNNNNNNNGG 3 cut(s) 201, 343, 344
BseMI GCAATG 1 cut(s) 579
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 2 cut(s) 399, 402
BseSI GKGCMC 1 cut(s) 443
BseXI GCAGC 7 cut(s) 338, 397, 448, 656, 680, 815, 988
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 663
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 1 cut(s) 151
BshNI GGYRCC 1 cut(s) 908
BsiHKAI GWGCWC 2 cut(s) 262, 443
BsiSI CCGG 1 cut(s) 906
BslFI GGGAC 5 cut(s) 77, 319, 388, 736, 949
BslI CCNNNNNNNGG 3 cut(s) 201, 343, 344
BsmAI GTCTC 3 cut(s) 19, 77, 620
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 5 cut(s) 77, 319, 388, 736, 949
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 262, 443
Bsp143I GATC 6 cut(s) 56, 87, 453, 544, 682, 927
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 6 cut(s) 26, 40, 324, 426, 912, 967
BspANI GGCC 1 cut(s) 151
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 837, 910
BspMAI CTGCAG 2 cut(s) 607, 978
BspMI ACCTGC 3 cut(s) 320, 905, 981
BspPI GGATC 3 cut(s) 461, 539, 690
BspT107I GGYRCC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 579
BsrFI RCCGGY 1 cut(s) 905
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 1 cut(s) 650
BssMI GATC 6 cut(s) 56, 87, 453, 544, 682, 927
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 5 cut(s) 106, 140, 617, 664, 892
BstACI GRCGYC 1 cut(s) 474
BstAPI GCANNNNNTGC 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 607
BstDSI CCRYGG 1 cut(s) 650
BstEII GGTNACC 1 cut(s) 332
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 6 cut(s) 59, 90, 456, 547, 685, 930
BstMAI GTCTC 3 cut(s) 19, 77, 620
BstMBI GATC 6 cut(s) 56, 87, 453, 544, 682, 927
BstMWI GCNNNNNNNGC 9 cut(s) 222, 323, 407, 560, 569, 644, 902, 911, 917
BstNI CCWGG 1 cut(s) 833
BstPAI GACNNNNGTC 1 cut(s) 728
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 831
BstSFI CTRYAG 4 cut(s) 603, 621, 633, 974
BstSLI GKGCMC 1 cut(s) 443
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 7 cut(s) 338, 397, 448, 656, 680, 815, 988
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 2 cut(s) 56, 453
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 2 cut(s) 56, 453
BsuI GTATCC 1 cut(s) 579
BsuRI GGCC 1 cut(s) 151
BtgI CCRYGG 1 cut(s) 650
BtsIMutI CAGTG 2 cut(s) 252, 747
BveI ACCTGC 3 cut(s) 320, 905, 981
Cac8I GCNNGC 1 cut(s) 607
CfoI GCGC 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 3 cut(s) 185, 617, 835
CseI GACGC 2 cut(s) 482, 733
CviAII CATG 3 cut(s) 302, 569, 651
DpnI GATC 6 cut(s) 58, 89, 455, 546, 684, 929
DpnII GATC 6 cut(s) 56, 87, 453, 544, 682, 927
DraI TTTAAA 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 650
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 3 cut(s) 185, 617, 835
Eco53kI GAGCTC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 429
Eco91I GGTNACC 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 831
EcoT14I CCWWGG 1 cut(s) 650
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 650
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 3 cut(s) 305, 572, 654
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 760, 792
FaqI GGGAC 5 cut(s) 77, 319, 388, 736, 949
FatI CATG 3 cut(s) 301, 568, 650
FauI CCCGC 1 cut(s) 974
Fnu4HI GCNGC 8 cut(s) 327, 411, 462, 645, 669, 829, 912, 977
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 8 cut(s) 327, 411, 462, 645, 669, 829, 912, 977
FspBI CTAG 2 cut(s) 285, 365
GlaI GCGC 1 cut(s) 29
GluI GCNGC 8 cut(s) 327, 411, 462, 645, 669, 829, 912, 977
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 1 cut(s) 151
HapII CCGG 1 cut(s) 906
HgaI GACGC 2 cut(s) 482, 733
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 3 cut(s) 305, 572, 654
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 5 cut(s) 144, 155, 275, 307, 655
HpaII CCGG 1 cut(s) 906
Hpy166II GTNNAC 2 cut(s) 441, 493
Hpy188I TCNGA 4 cut(s) 160, 448, 690, 734
Hpy8I GTNNAC 2 cut(s) 441, 493
Hpy99I CGWCG 2 cut(s) 488, 690
HpyAV CCTTC 3 cut(s) 53, 170, 779
HpyCH4III ACNGT 5 cut(s) 106, 140, 617, 664, 892
HpyCH4IV ACGT 1 cut(s) 822
HpyF10VI GCNNNNNNNGC 9 cut(s) 222, 323, 407, 560, 569, 644, 902, 911, 917
HpySE526I ACGT 1 cut(s) 822
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 3 cut(s) 305, 572, 654
HspAI GCGC 1 cut(s) 28
Kzo9I GATC 6 cut(s) 56, 87, 453, 544, 682, 927
LmnI GCTCC 2 cut(s) 257, 764
Lsp1109I GCAGC 7 cut(s) 338, 397, 448, 656, 680, 815, 988
MaeI CTAG 2 cut(s) 285, 365
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 2 cut(s) 332, 818
MalI GATC 6 cut(s) 58, 89, 455, 546, 684, 929
MboI GATC 6 cut(s) 56, 87, 453, 544, 682, 927
MboII GAAGA 1 cut(s) 863
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 2 cut(s) 56, 453
MhlI GDGCHC 2 cut(s) 262, 443
MluCI AATT 9 cut(s) 33, 229, 405, 504, 510, 693, 755, 841, 921
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 2 cut(s) 668, 712
MnlI CCTC 8 cut(s) 377, 380, 406, 509, 604, 713, 1030, 1049
MroXI GAANNNNTTC 1 cut(s) 749
MseI TTAA 2 cut(s) 168, 758
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 1 cut(s) 833
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 1 cut(s) 833
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 9 cut(s) 222, 323, 407, 560, 569, 644, 902, 911, 917
NcoI CCATGG 1 cut(s) 650
NdeII GATC 6 cut(s) 56, 87, 453, 544, 682, 927
NlaIII CATG 3 cut(s) 305, 572, 654
NlaIV GGNNCC 2 cut(s) 837, 910
PaqCI CACCTGC 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 4 cut(s) 144, 155, 275, 655
PflMI CCANNNNNTGG 1 cut(s) 201
PkrI GCNGC 8 cut(s) 328, 412, 463, 646, 670, 830, 913, 978
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PshAI GACNNNNGTC 1 cut(s) 728
Psp124BI GAGCTC 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 831
PspEI GGTNACC 1 cut(s) 332
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 2 cut(s) 837, 910
PspPI GGNCC 3 cut(s) 185, 617, 835
PstI CTGCAG 2 cut(s) 607, 978
PsuI RGATCY 2 cut(s) 56, 453
SacI GAGCTC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 168, 758
SatI GCNGC 8 cut(s) 327, 411, 462, 645, 669, 829, 912, 977
Sau3AI GATC 6 cut(s) 56, 87, 453, 544, 682, 927
Sau96I GGNCC 3 cut(s) 185, 617, 835
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 833
SduI GDGCHC 2 cut(s) 262, 443
SfcI CTRYAG 4 cut(s) 603, 621, 633, 974
SinI GGWCC 3 cut(s) 185, 617, 835
SmlI CTYRAG 2 cut(s) 432, 1040
SmoI CTYRAG 2 cut(s) 432, 1040
Sse9I AATT 9 cut(s) 33, 229, 405, 504, 510, 693, 755, 841, 921
SsiI CCGC 6 cut(s) 26, 40, 324, 426, 912, 967
SspMI CTAG 2 cut(s) 285, 365
SstI GAGCTC 1 cut(s) 262
StyD4I CCNGG 1 cut(s) 831
StyI CCWWGG 1 cut(s) 650
TaaI ACNGT 5 cut(s) 106, 140, 617, 664, 892
TaiI ACGT 1 cut(s) 825
TaqI TCGA 1 cut(s) 72
TaqII GACCGA 1 cut(s) 252
TasI AATT 9 cut(s) 33, 229, 405, 504, 510, 693, 755, 841, 921
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 4 cut(s) 144, 155, 275, 655
Tru1I TTAA 2 cut(s) 168, 758
Tru9I TTAA 2 cut(s) 168, 758
TscAI CASTG 2 cut(s) 259, 747
TseI GCWGC 7 cut(s) 326, 410, 461, 644, 668, 828, 976
TspDTI ATGAA 5 cut(s) 17, 58, 557, 915, 948
TspGWI ACGGA 2 cut(s) 501, 695
TspRI CASTG 2 cut(s) 259, 747
Van91I CCANNNNNTGG 1 cut(s) 201
VneI GTGCAC 1 cut(s) 439
VpaK11BI GGWCC 3 cut(s) 185, 617, 835
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 285, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.