Rh5BG455900

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000257
Physical Location & Seq
Forward (+)
72986406 .. 72988650
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG455900.1

Sequence Viewer

Length: 900 bp
ATGACAAAGCATGGAGCTCTTCTGTTTTATCACACTTTGTCAATTCTCATCCTCACTCTGAGTTTGTTATGCAGAGCCACTTGCGACGATAGAAAGGTCTATATTGTGTACTTGGGGTCACTTTCTGATGGGTTGTACTCGCCGTCATCTCACCATCTTAGTATTCTACAGAGAGTTGTCCAGGACAGTTCTCCTGAAAATGTTTTGGTAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAGACTCACTGACCAGGAAAGAGAAAAACTTTCTAAAATGAAGGAAGTAGTCTCTGTTTTTCCAAGCAGAACATTGCAACTTCAAACAACAAGATCTTGGGACTTCATGGGATTCAATGAGAAAATTGGTCGAAATGCCACAGTTGAGAGTGATATTATTGTGGGTGTCATTGACAGTGGAATTTGGCCTGAATCTGAGAGTTTTAATGATGAAGGTTTTGGTCCTCCACCCAAGAAGTGGAAAGTTGCAGCAAGTAGCATGGACCGTCGGATCATTGACAAGGTTATCCTTGGAAATGGAAGGACAGTAATTGGGAATTCTGTGAACTCCTTCACATTAAATGGTACAAGTTTTCCTCTGATACATGGAAAAAATGCTTCAAAGAACTGCACAGAAACAATTGCGGGAGATTGTGAACAAGGTTGCCTGGACAGTAGTCTAGTGAAGGGAAAGGTTGTGCTATGTGATAAATATGGACTTCTTGAAGCATTTCAATCTGGTGCACTGGGTTCAATTTTACCCAATTTTCTAGCTGTTGATGATGCCTCTTTTGTTCTCCCATTAGCTGGCACGACTATAAACAACGAAAACTATAATGTAGTCATGTCATACTTGAACTCCACCAGGTTGAGATTATTTTGGATACAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

299

Amino Acids

33.1

Weight (kDa)

8.47

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 110 4.3e-18 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 483, 812
AciI CCGC 1 cut(s) 650
AclWI GGATC 1 cut(s) 524
AcsI RAATTY 2 cut(s) 426, 562
AfaI GTAC 3 cut(s) 110, 137, 592
AfiI CCNNNNNNNGG 2 cut(s) 483, 812
AgsI TTSAA 8 cut(s) 232, 329, 361, 627, 731, 740, 759, 862
AjnI CCWGG 4 cut(s) 180, 258, 672, 869
AluBI AGCT 3 cut(s) 17, 779, 812
AluI AGCT 3 cut(s) 17, 779, 812
Alw21I GWGCWC 2 cut(s) 19, 751
Alw26I GTCTC 1 cut(s) 301
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 524
AoxI GGCC 1 cut(s) 431
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 2 cut(s) 242, 494
ApoI RAATTY 2 cut(s) 426, 562
Asp700I GAANNNNTTC 2 cut(s) 575, 735
AspS9I GGNCC 2 cut(s) 467, 508
AsuHPI GGTGA 1 cut(s) 143
AvaII GGWCC 2 cut(s) 467, 508
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 1 cut(s) 881
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 19, 751
BbvI GCAGC 2 cut(s) 229, 506
BccI CCATC 2 cut(s) 122, 162
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 4 cut(s) 182, 260, 674, 871
BciVI GTATCC 1 cut(s) 882
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 686, 776
BfmI CTRYAG 1 cut(s) 167
BfuI GTATCC 1 cut(s) 882
BglII AGATCT 1 cut(s) 338
BisI GCNGC 2 cut(s) 243, 495
BlsI GCNGC 2 cut(s) 244, 496
Bme1390I CCNGG 4 cut(s) 182, 260, 674, 871
Bme18I GGWCC 2 cut(s) 467, 508
BmgT120I GGNCC 2 cut(s) 467, 508
BmrFI CCNGG 4 cut(s) 182, 260, 674, 871
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 1 cut(s) 778
BmuI ACTGGG 1 cut(s) 761
BoxI GACNNNNGTC 1 cut(s) 681
BsaJI CCNNGG 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 848, 878
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 812
Bse1I ACTGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 317
BseBI CCWGG 4 cut(s) 182, 260, 674, 871
BseDI CCNNGG 1 cut(s) 535
BseGI GGATG 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 483, 812
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 2 cut(s) 50, 432
BseNI ACTGG 1 cut(s) 756
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 2 cut(s) 229, 506
BsgI GTGCAG 1 cut(s) 619
BshFI GGCC 1 cut(s) 433
BsiHKAI GWGCWC 2 cut(s) 19, 751
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 2 cut(s) 483, 812
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 359
BsnI GGCC 1 cut(s) 433
Bsp1286I GDGCHC 2 cut(s) 19, 751
Bsp143I GATC 2 cut(s) 338, 516
BspACI CCGC 1 cut(s) 650
BspANI GGCC 1 cut(s) 433
BspCNI CTCAG 2 cut(s) 51, 433
BspPI GGATC 1 cut(s) 524
BspQI GCTCTTC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 317
BsrI ACTGG 1 cut(s) 756
BssECI CCNNGG 1 cut(s) 535
BssMI GATC 2 cut(s) 338, 516
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 4 cut(s) 182, 260, 674, 871
Bst4CI ACNGT 6 cut(s) 188, 388, 422, 512, 553, 680
Bst6I CTCTTC 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 814
BstDEI CTNAG 3 cut(s) 59, 158, 441
BstF5I GGATG 1 cut(s) 48
BstKTI GATC 2 cut(s) 341, 519
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 2 cut(s) 338, 516
BstNI CCWGG 4 cut(s) 182, 260, 674, 871
BstPAI GACNNNNGTC 1 cut(s) 681
BstSCI CCNGG 4 cut(s) 180, 258, 672, 869
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 2 cut(s) 229, 506
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 1 cut(s) 433
BtsCI GGATG 1 cut(s) 48
BtsIMutI CAGTG 3 cut(s) 252, 427, 749
Cac8I GCNNGC 1 cut(s) 814
Cfr13I GGNCC 2 cut(s) 467, 508
CsiI ACCWGGT 1 cut(s) 869
Csp6I GTAC 3 cut(s) 109, 136, 591
CviAII CATG 5 cut(s) 11, 352, 505, 611, 850
CviJI RGCY 5 cut(s) 17, 77, 433, 779, 812
CviKI_1 RGCY 5 cut(s) 17, 77, 433, 779, 812
CviQI GTAC 3 cut(s) 109, 136, 591
DdeI CTNAG 3 cut(s) 59, 158, 441
DpnI GATC 2 cut(s) 340, 518
DpnII GATC 2 cut(s) 338, 516
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 2 cut(s) 467, 508
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 562
EcoRII CCWGG 4 cut(s) 180, 258, 672, 869
EcoT14I CCWWGG 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 5 cut(s) 14, 355, 508, 614, 853
FaqI GGGAC 1 cut(s) 359
FatI CATG 5 cut(s) 10, 351, 504, 610, 849
FauI CCCGC 1 cut(s) 643
Fnu4HI GCNGC 2 cut(s) 243, 495
FokI GGATG 1 cut(s) 35
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 243, 495
FspBI CTAG 2 cut(s) 686, 776
GluI GCNGC 2 cut(s) 243, 495
HaeIII GGCC 1 cut(s) 433
Hin1II CATG 5 cut(s) 14, 355, 508, 614, 853
HinfI GANTC 3 cut(s) 249, 357, 437
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 4 cut(s) 109, 571, 662, 749
Hpy188I TCNGA 5 cut(s) 60, 127, 442, 516, 606
Hpy188III TCNNGA 2 cut(s) 194, 728
Hpy8I GTNNAC 4 cut(s) 109, 571, 662, 749
Hpy99I CGWCG 2 cut(s) 89, 516
HpyAV CCTTC 5 cut(s) 280, 452, 540, 586, 685
HpyCH4III ACNGT 6 cut(s) 188, 388, 422, 512, 553, 680
HpyCH4V TGCA 5 cut(s) 72, 322, 494, 636, 749
HpyF3I CTNAG 3 cut(s) 59, 158, 441
Hsp92II CATG 5 cut(s) 14, 355, 508, 614, 853
Kzo9I GATC 2 cut(s) 338, 516
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 2 cut(s) 229, 506
LweI GCATC 1 cut(s) 778
MabI ACCWGGT 1 cut(s) 869
MaeI CTAG 2 cut(s) 686, 776
MaeIII GTNAC 2 cut(s) 117, 215
MalI GATC 2 cut(s) 340, 518
MboI GATC 2 cut(s) 338, 516
MboII GAAGA 1 cut(s) 11
MfeI CAATTG 1 cut(s) 645
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 2 cut(s) 19, 751
MluCI AATT 8 cut(s) 42, 369, 426, 555, 562, 645, 759, 769
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 4 cut(s) 62, 480, 612, 802
MroXI GAANNNNTTC 2 cut(s) 575, 735
MseI TTAA 2 cut(s) 450, 584
MspR9I CCNGG 4 cut(s) 182, 260, 674, 871
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 4 cut(s) 182, 260, 674, 871
NdeII GATC 2 cut(s) 338, 516
NlaIII CATG 5 cut(s) 14, 355, 508, 614, 853
NmuCI GTSAC 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 575, 735
PfeI GAWTC 2 cut(s) 357, 437
PflMI CCANNNNNTGG 2 cut(s) 483, 812
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 2 cut(s) 244, 496
PleI GAGTC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 243
PshAI GACNNNNGTC 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 4 cut(s) 180, 258, 672, 869
PspGI CCWGG 4 cut(s) 180, 258, 672, 869
PspPI GGNCC 2 cut(s) 467, 508
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 3 cut(s) 110, 137, 592
RsaNI GTAC 3 cut(s) 109, 136, 591
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 2 cut(s) 450, 584
SatI GCNGC 2 cut(s) 243, 495
Sau3AI GATC 2 cut(s) 338, 516
Sau96I GGNCC 2 cut(s) 467, 508
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 4 cut(s) 182, 260, 674, 871
SduI GDGCHC 2 cut(s) 19, 751
SexAI ACCWGGT 1 cut(s) 869
SfaNI GCATC 1 cut(s) 778
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 2 cut(s) 467, 508
Sse9I AATT 8 cut(s) 42, 369, 426, 555, 562, 645, 759, 769
SsiI CCGC 1 cut(s) 650
SspMI CTAG 2 cut(s) 686, 776
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 4 cut(s) 180, 258, 672, 869
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 6 cut(s) 188, 388, 422, 512, 553, 680
TaqI TCGA 1 cut(s) 376
TasI AATT 8 cut(s) 42, 369, 426, 555, 562, 645, 759, 769
TatI WGTACW 2 cut(s) 108, 135
TfiI GAWTC 2 cut(s) 357, 437
Tru1I TTAA 2 cut(s) 450, 584
Tru9I TTAA 2 cut(s) 450, 584
TscAI CASTG 3 cut(s) 259, 427, 756
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 2 cut(s) 242, 494
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 3 cut(s) 299, 340, 471
TspRI CASTG 3 cut(s) 259, 427, 756
Van91I CCANNNNNTGG 2 cut(s) 483, 812
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 467, 508
XapI RAATTY 2 cut(s) 426, 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 480
XmnI GAANNNNTTC 2 cut(s) 575, 735
XspI CTAG 2 cut(s) 686, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.