Rroxscaffold_4G00325970

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000257
Physical Location & Seq
Reverse (-)
58021442 .. 58022810
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325970.1

Sequence Viewer

Length: 1353 bp
ATGGCTAAGCATGGAGCTCTCCTATTTTCTTATGCTTTCACCATTCTCATACTCACTTTGAACTTATTGTGCAATGCCGTTGATGAAGATAGACATGCTCATGTTGTGTACTTAGGGTCACTTCCAGATGACAAACTGTACTCACCGTTGTCTCACCACCTTAGCATTCTACAAAGAGTAACAGGGAGAGAATCTGCTGCAAATTTGTTAATAAAAAATTACAAAAGAAGTTTCAATGGATTTTTTGCCAATCTCACTGAACACGAAAGAGAAGAGCTTGCTAACATGAAGGAAGTAGTCTCTGTCTTTCCAAGCACAACTTTCCAACTTCAAACAACACGATCATGGGAGTTCATGGGCTTCAATGAGAAAATCAGAGGGAATGCCATTGTTGAGAGTGATGTCATCATTGGTGTTCTTGACACTGGAATTTGGCATGAATCCGAGAGCTTTAAAGATGAAGGTTTCGGTCCTGCTCCCAAGAAGTGGAAAGGTACTTGTAAAGGTGGTCAAAATTTCACTTACAACAACAAGCTCATTGGAGCTCAGTTTTACTCATCATCAGACTCGGCAAAGGATGTAATAGGTCATGGGACCCACACTGCCTCAACAGTAGCAGGGAACTTAGTAAAAGATGTAAGCTTTTATGGACTAGCACAAGGTACCGCAAGAGGAGGAGTTCCCTCTGCTACAGTTTCTGCATATAGTGTCTGCAGTGCAGACGGGTGCAAATCATACGATATGCTGGCTGCTTTTGATGATGCTATTGCTGATGGAGTTGACATCATTACAGTTTCACTTGGAGCATCTTTGGCAATTCCAATCGCTCACGATCCTATAGCAATCGGTTCTTATCATGCAATACAAAAAGGGATACTAACCACAAATGCTGCGGGCAACAATGGTCGTATTGGTGGTACTGTCTCAAGTGTTGCACCATGGATGCTCATTGTTGCAGCCAGTAGCATGGATCATGGGATCATTGACAAAGTTGCTCTTGGAAATGGAAGGACACTTGTTGGGAATTCTGTCAACTCTTTCACATCAAATGAAACAATAGAGGGAAAAGATGTTCGTAATAAATGTTGCACTGAAGTCCAAGCTGGGCATTGTGAAGATTTGTGCATAGATAATGATGCAGTAAAAGGAAAGATTGTGTTATGCGATGAATTAACTGGAAATGTTGAGGCCCATAAAGCTGGTGCAATTGGCACAGTTTTAAAGAATAATATACCAGCTTTTGAAGGAGTTTCTGAAGTTCTCCCACTACCAGCAGTAGCTTTAAGGGATGAAGAGTATAATGTTGTCAAGTCCTACATGAACTCCACTAAACATCCTCGAGCCAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

450

Amino Acids

48.15

Weight (kDa)

5.83

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 111 2.3e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 133 - 324 5.7e-17 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 486
AciI CCGC 2 cut(s) 666, 893
AclWI GGATC 3 cut(s) 827, 978, 986
AcsI RAATTY 4 cut(s) 202, 429, 514, 1024
AcuI CTGAAG 2 cut(s) 1113, 1275
AfaI GTAC 5 cut(s) 110, 140, 496, 664, 919
AfiI CCNNNNNNNGG 1 cut(s) 486
AgsI TTSAA 5 cut(s) 61, 235, 332, 364, 1244
AloI GAACNNNNNNTCC 2 cut(s) 1056, 1088
Alw21I GWGCWC 2 cut(s) 19, 547
Alw26I GTCTC 3 cut(s) 156, 304, 928
AlwI GGATC 3 cut(s) 827, 978, 986
AlwNI CAGNNNCTG 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 1338
AoxI GGCC 1 cut(s) 1188
ApeKI GCWGC 4 cut(s) 197, 749, 890, 956
ApoI RAATTY 4 cut(s) 202, 429, 514, 1024
Asp718I GGTACC 1 cut(s) 662
AspS9I GGNCC 3 cut(s) 470, 594, 1189
AsuHPI GGTGA 3 cut(s) 31, 135, 146
AvaI CYCGRG 1 cut(s) 1338
AvaII GGWCC 2 cut(s) 470, 594
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 2 cut(s) 19, 547
Bbv12I GWGCWC 2 cut(s) 19, 547
BbvI GCAGC 4 cut(s) 184, 736, 877, 968
BccI CCATC 1 cut(s) 767
BceAI ACGGC 1 cut(s) 62
BciVI GTATCC 1 cut(s) 867
BcoDI GTCTC 3 cut(s) 156, 304, 928
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 3 cut(s) 690, 712, 837
BfuI GTATCC 1 cut(s) 867
BisI GCNGC 4 cut(s) 198, 750, 891, 957
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 4 cut(s) 199, 751, 892, 958
Bme18I GGWCC 2 cut(s) 470, 594
BmeT110I CYCGRG 1 cut(s) 1338
BmgT120I GGNCC 3 cut(s) 470, 594, 1189
BmiI GGNNCC 3 cut(s) 595, 596, 664
BmsI GCATC 4 cut(s) 751, 815, 933, 1126
Bpu10I CCTNAGC 1 cut(s) 161
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 910
BsaJI CCNNGG 1 cut(s) 938
BsaXI ACNNNNNCTCC 2 cut(s) 1307, 1337
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse1I ACTGG 3 cut(s) 430, 960, 1180
Bse3DI GCAATG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 938
BseGI GGATG 4 cut(s) 583, 948, 1294, 1333
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 560
BseNI ACTGG 3 cut(s) 430, 960, 1180
BseRI GAGGAG 2 cut(s) 687, 690
BseXI GCAGC 4 cut(s) 184, 736, 877, 968
BseYI CCCAGC 1 cut(s) 1103
BsgI GTGCAG 1 cut(s) 738
BshFI GGCC 1 cut(s) 1190
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 2 cut(s) 19, 547
BsiHKCI CYCGRG 1 cut(s) 1338
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 3 cut(s) 156, 304, 928
BsmFI GGGAC 1 cut(s) 607
BsmI GAATGC 2 cut(s) 165, 388
BsnI GGCC 1 cut(s) 1190
BsoBI CYCGRG 1 cut(s) 1338
Bsp1286I GDGCHC 2 cut(s) 19, 547
Bsp143I GATC 4 cut(s) 341, 832, 970, 978
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 2 cut(s) 666, 893
BspANI GGCC 1 cut(s) 1190
BspCNI CTCAG 1 cut(s) 559
BspLI GGNNCC 3 cut(s) 595, 596, 664
BspMAI CTGCAG 1 cut(s) 716
BspPI GGATC 3 cut(s) 827, 978, 986
BspQI GCTCTTC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 3 cut(s) 430, 960, 1180
BssECI CCNNGG 1 cut(s) 938
BssMI GATC 4 cut(s) 341, 832, 970, 978
BssT1I CCWWGG 1 cut(s) 938
Bst4CI ACNGT 7 cut(s) 138, 147, 613, 694, 793, 922, 1216
Bst6I CTCTTC 2 cut(s) 267, 1287
BstC8I GCNNGC 3 cut(s) 279, 747, 895
BstDEI CTNAG 5 cut(s) 6, 112, 161, 546, 625
BstDSI CCRYGG 1 cut(s) 938
BstF5I GGATG 4 cut(s) 583, 948, 1294, 1333
BstKTI GATC 4 cut(s) 344, 835, 973, 981
BstMAI GTCTC 3 cut(s) 156, 304, 928
BstMBI GATC 4 cut(s) 341, 832, 970, 978
BstMWI GCNNNNNNNGC 2 cut(s) 812, 1196
BstNSI RCATGY 1 cut(s) 98
BstSFI CTRYAG 3 cut(s) 690, 712, 837
BstV1I GCAGC 4 cut(s) 184, 736, 877, 968
BstXI CCANNNNNNTGG 2 cut(s) 967, 1199
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 1 cut(s) 1190
BtgI CCRYGG 1 cut(s) 938
BtgZI GCGATG 1 cut(s) 1179
BtsCI GGATG 4 cut(s) 583, 948, 1294, 1333
BtsI GCAGTG 2 cut(s) 600, 721
BtsIMutI CAGTG 5 cut(s) 255, 423, 600, 721, 1089
Cac8I GCNNGC 3 cut(s) 279, 747, 895
CaiI CAGNNNCTG 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 470, 594, 1189
Csp6I GTAC 5 cut(s) 109, 139, 495, 663, 918
CviQI GTAC 5 cut(s) 109, 139, 495, 663, 918
DdeI CTNAG 5 cut(s) 6, 112, 161, 546, 625
DpnI GATC 4 cut(s) 343, 834, 972, 980
DpnII GATC 4 cut(s) 341, 832, 970, 978
DraI TTTAAA 2 cut(s) 454, 1221
Eam1104I CTCTTC 2 cut(s) 267, 1287
EarI CTCTTC 2 cut(s) 267, 1287
Ecl136II GAGCTC 2 cut(s) 17, 545
Eco130I CCWWGG 1 cut(s) 938
Eco24I GRGCYC 2 cut(s) 19, 547
Eco47I GGWCC 2 cut(s) 470, 594
Eco53kI GAGCTC 2 cut(s) 17, 545
Eco57I CTGAAG 2 cut(s) 1113, 1275
Eco88I CYCGRG 1 cut(s) 1338
EcoICRI GAGCTC 2 cut(s) 17, 545
EcoO109I RGGNCCY 1 cut(s) 594
EcoRI GAATTC 1 cut(s) 1024
EcoT14I CCWWGG 1 cut(s) 938
EcoT38I GRGCYC 2 cut(s) 19, 547
ErhI CCWWGG 1 cut(s) 938
FalI AAGNNNNNCTT 4 cut(s) 304, 336, 981, 1013
FaqI GGGAC 1 cut(s) 607
FauI CCCGC 1 cut(s) 886
FauNDI CATATG 1 cut(s) 1349
Fnu4HI GCNGC 4 cut(s) 198, 750, 891, 957
FokI GGATG 4 cut(s) 590, 955, 1301, 1320
FriOI GRGCYC 2 cut(s) 19, 547
Fsp4HI GCNGC 4 cut(s) 198, 750, 891, 957
FspBI CTAG 1 cut(s) 653
GluI GCNGC 4 cut(s) 198, 750, 891, 957
GsaI CCCAGC 1 cut(s) 1107
HaeIII GGCC 1 cut(s) 1190
HincII GTYRAC 2 cut(s) 781, 1033
HindII GTYRAC 2 cut(s) 781, 1033
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 3 cut(s) 191, 440, 566
HphI GGTGA 3 cut(s) 31, 135, 146
Hpy166II GTNNAC 3 cut(s) 109, 781, 1033
Hpy188I TCNGA 4 cut(s) 377, 445, 565, 1255
Hpy188III TCNNGA 3 cut(s) 125, 419, 830
Hpy8I GTNNAC 3 cut(s) 109, 781, 1033
HpyAV CCTTC 4 cut(s) 283, 455, 1002, 1238
HpyCH4III ACNGT 7 cut(s) 138, 147, 613, 694, 793, 922, 1216
HpyF10VI GCNNNNNNNGC 2 cut(s) 812, 1196
HpyF3I CTNAG 5 cut(s) 6, 112, 161, 546, 625
KflI GGGWCCC 1 cut(s) 594
KpnI GGTACC 1 cut(s) 666
Kzo9I GATC 4 cut(s) 341, 832, 970, 978
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 4 cut(s) 14, 481, 542, 803
Lsp1109I GCAGC 4 cut(s) 184, 736, 877, 968
LweI GCATC 4 cut(s) 751, 815, 933, 1126
MaeI CTAG 1 cut(s) 653
MaeIII GTNAC 2 cut(s) 117, 178
MalI GATC 4 cut(s) 343, 834, 972, 980
MboI GATC 4 cut(s) 341, 832, 970, 978
MboII GAAGA 4 cut(s) 98, 284, 1127, 1304
MfeI CAATTG 1 cut(s) 1206
MhlI GDGCHC 2 cut(s) 19, 547
MluCI AATT 8 cut(s) 202, 217, 429, 514, 816, 1024, 1169, 1206
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 8 cut(s) 371, 616, 665, 668, 694, 1054, 1180, 1347
MseI TTAA 5 cut(s) 209, 453, 1172, 1220, 1283
MslI CAYNNNNRTG 2 cut(s) 99, 343
MunI CAATTG 1 cut(s) 1206
Mva1269I GAATGC 2 cut(s) 165, 388
MwoI GCNNNNNNNGC 2 cut(s) 812, 1196
NcoI CCATGG 1 cut(s) 938
NdeI CATATG 1 cut(s) 1349
NdeII GATC 4 cut(s) 341, 832, 970, 978
NlaIV GGNNCC 3 cut(s) 595, 596, 664
NmeAIII GCCGAG 1 cut(s) 548
NmuCI GTSAC 1 cut(s) 117
NspI RCATGY 1 cut(s) 98
PaeR7I CTCGAG 1 cut(s) 1338
PciSI GCTCTTC 1 cut(s) 267
PctI GAATGC 2 cut(s) 165, 388
PfeI GAWTC 2 cut(s) 191, 440
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 4 cut(s) 199, 751, 892, 958
PleI GAGTC 1 cut(s) 560
PpsI GAGTC 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 594
Psp124BI GAGCTC 2 cut(s) 19, 547
Psp5II RGGWCCY 1 cut(s) 594
PspFI CCCAGC 1 cut(s) 1103
PspN4I GGNNCC 3 cut(s) 595, 596, 664
PspPI GGNCC 3 cut(s) 470, 594, 1189
PspPPI RGGWCCY 1 cut(s) 594
PspXI VCTCGAGB 1 cut(s) 1338
PstI CTGCAG 1 cut(s) 716
PstNI CAGNNNCTG 1 cut(s) 698
RsaI GTAC 5 cut(s) 110, 140, 496, 664, 919
RsaNI GTAC 5 cut(s) 109, 139, 495, 663, 918
RseI CAYNNNNRTG 2 cut(s) 99, 343
SacI GAGCTC 2 cut(s) 19, 547
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 5 cut(s) 209, 453, 1172, 1220, 1283
SatI GCNGC 4 cut(s) 198, 750, 891, 957
Sau3AI GATC 4 cut(s) 341, 832, 970, 978
Sau96I GGNCC 3 cut(s) 470, 594, 1189
SchI GAGTC 1 cut(s) 560
SduI GDGCHC 2 cut(s) 19, 547
SfaNI GCATC 4 cut(s) 751, 815, 933, 1126
SfcI CTRYAG 3 cut(s) 690, 712, 837
Sfr274I CTCGAG 1 cut(s) 1338
SinI GGWCC 2 cut(s) 470, 594
SlaI CTCGAG 1 cut(s) 1338
SmiMI CAYNNNNRTG 2 cut(s) 99, 343
SmlI CTYRAG 2 cut(s) 925, 1338
SmoI CTYRAG 2 cut(s) 925, 1338
Sse9I AATT 8 cut(s) 202, 217, 429, 514, 816, 1024, 1169, 1206
SsiI CCGC 2 cut(s) 666, 893
SspMI CTAG 1 cut(s) 653
SstI GAGCTC 2 cut(s) 19, 547
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 7 cut(s) 138, 147, 613, 694, 793, 922, 1216
TaqI TCGA 1 cut(s) 1339
TaqII GACCGA 1 cut(s) 458
TasI AATT 8 cut(s) 202, 217, 429, 514, 816, 1024, 1169, 1206
TatI WGTACW 2 cut(s) 108, 138
TfiI GAWTC 2 cut(s) 191, 440
Tru1I TTAA 5 cut(s) 209, 453, 1172, 1220, 1283
Tru9I TTAA 5 cut(s) 209, 453, 1172, 1220, 1283
TscAI CASTG 5 cut(s) 262, 430, 607, 721, 1096
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 4 cut(s) 197, 749, 890, 956
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 9 cut(s) 99, 302, 343, 453, 474, 1065, 1182, 1305, 1334
TspRI CASTG 5 cut(s) 262, 430, 607, 721, 1096
Van91I CCANNNNNTGG 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 470, 594
XapI RAATTY 4 cut(s) 202, 429, 514, 1024
XceI RCATGY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 1338
XspI CTAG 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.