MD03G1083800.v1.1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000257
Physical Location & Seq
Reverse (-)
6782787 .. 6785421
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1083800.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGATAGAAAGGTCTACATCGGGTACTTGGGGGTCGCTTTCTGGTCGCGTGTACTCACCATTGTTGTCTCAACACCTTAATATCCTAGAGAGAGTTGTCCAGGGAAGCAATTCTGCTGAAAGCTTATTGGTAAGGAGTTTCAATGGATTTGCTGCCTATCTAACTGACCAGGAAAGGGAAAAACTTGCTAATATGAAGGGAGTGGTCTCTGTCTTTCCGAGCAGAGAATTGCAACTTCAAACAACAAGATCTTGGGACTTTATGGGACTCAGTGAGAAATTCAATCGAAGTCCCTCTGTTGAGAGTGATGCTGTTGTTGGTGTGATCAACAGTGGAATTTGGCCAGAGTCTGAGAGCTTTAAAGATGAAGGTCTTGGTCCTCCTCCGAAAAAATGGAAAGGTGCTTGTGAAGGCGGCAAAAATTTCACTTGCAACAACAAGCTCATTGGGGCGCGATATTATATAACAGAGTCTGCAAGGGATGAATGGTGTCATGGAACCCACACTGCCTCAACGACAGCAGGGAACGCTGTAAAGGATGTGAGCTTTTATGGACTAGCAGAAGGTACTGCAAGAGGAGGTGTTCCTGCTGCGAGAATTGCAGCATACAAAGTACGCTCGCTGGCACAATCGTGCAGTGCACATAATACGTTGGCTGCTTTTGACGATGCTATTGCTGATGGAGTCGACATTATTTCAATTTCAATTGCTTTTTCTTCTATAAATTTTTTTGATGAGGATCCTATTGCAATTGGTGCTTTTCATGCGATGAAGAAAGGGATACTGACATCAAATGCTGCGGGTAATAATGGTCCTTCGGGTGCTACTGTTTCAAGTGTAGCACCATGGATGCTTACAGTTGCAACAAGTAGCATGGATCGTAGGATCATTGATAAAGTTGTTCTAGGAAATGGACAGACACTAGTTGTATGTATCAGTGACTTCCTCTATCATTTACCAATAAACTTATATATTTCTAATTTTTTGTTATATTGTTTTCAACAGTATTTACACAAACAGGGGAATTCAGTGAACACTTTCACATTAAATGGAACAAGGTTTCCTCTGGTTCATGGAAAGGATGCTTCAAGTTCAAGTAATTGCACGGAACAAGACGCCAGAAATTGTGAACTAGGTTGTCTGGACAGTACATTAGTGAAGGAAAAGATTGTGCTATGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

395

Amino Acids

42.59

Weight (kDa)

6.26

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 27 - 80 1.9e-08 Peptidase inhibitor I9
Peptidase_S8 PF00082 103 - 351 3.4e-18 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 14, 687
AccII CGCG 2 cut(s) 48, 454
AciI CCGC 2 cut(s) 414, 800
AclWI GGATC 4 cut(s) 734, 747, 885, 893
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 5 cut(s) 278, 336, 421, 724, 1024
AcyI GRCGYC 1 cut(s) 1116
AfaI GTAC 5 cut(s) 25, 53, 568, 615, 1150
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 9 cut(s) 142, 239, 283, 699, 705, 834, 1001, 1089, 1095
AhlI ACTAGT 1 cut(s) 922
AjnI CCWGG 2 cut(s) 99, 168
AleI CACNNNNGTG 1 cut(s) 631
AloI GAACNNNNNNTCC 2 cut(s) 1045, 1077
AluBI AGCT 4 cut(s) 123, 357, 442, 546
AluI AGCT 4 cut(s) 123, 357, 442, 546
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 2 cut(s) 72, 211
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 4 cut(s) 734, 747, 885, 893
AlwNI CAGNNNCTG 2 cut(s) 350, 473
AoxI GGCC 1 cut(s) 341
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 5 cut(s) 152, 590, 602, 656, 797
ApoI RAATTY 5 cut(s) 278, 336, 421, 724, 1024
Asp700I GAANNNNTTC 2 cut(s) 109, 1037
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 2 cut(s) 377, 812
AsuHPI GGTGA 1 cut(s) 48
AvaII GGWCC 2 cut(s) 377, 812
BaeGI GKGCMC 1 cut(s) 643
BalI TGGCCA 1 cut(s) 343
BamHI GGATCC 1 cut(s) 739
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 5 cut(s) 139, 577, 614, 643, 784
BccI CCATC 1 cut(s) 674
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 2 cut(s) 101, 170
BciVI GTATCC 1 cut(s) 774
BclI TGATCA 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 72, 211
BcuI ACTAGT 1 cut(s) 922
BfaI CTAG 5 cut(s) 86, 557, 905, 923, 1133
BfuI GTATCC 1 cut(s) 774
BglII AGATCT 1 cut(s) 248
BisI GCNGC 6 cut(s) 153, 415, 591, 603, 657, 798
BlsI GCNGC 6 cut(s) 154, 416, 592, 604, 658, 799
Bme1390I CCNGG 2 cut(s) 101, 170
Bme18I GGWCC 2 cut(s) 377, 812
BmgT120I GGNCC 2 cut(s) 377, 812
BmiI GGNNCC 2 cut(s) 499, 741
BmrFI CCNGG 2 cut(s) 101, 170
BmsI GCATC 4 cut(s) 298, 658, 840, 1072
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 1 cut(s) 1116
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 2 cut(s) 100, 845
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 2 cut(s) 101, 170
BseDI CCNNGG 2 cut(s) 100, 845
BseGI GGATG 4 cut(s) 487, 544, 855, 1087
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 283, 342
BseRI GAGGAG 2 cut(s) 372, 591
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 5 cut(s) 139, 577, 614, 643, 784
BsgI GTGCAG 1 cut(s) 655
Bsh1236I CGCG 2 cut(s) 48, 454
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 643
BslFI GGGAC 3 cut(s) 269, 276, 279
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 2 cut(s) 72, 211
BsmFI GGGAC 3 cut(s) 269, 276, 279
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 5 cut(s) 248, 324, 739, 877, 885
Bsp19I CCATGG 1 cut(s) 845
BspACI CCGC 2 cut(s) 414, 800
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 2 cut(s) 282, 343
BspFNI CGCG 2 cut(s) 48, 454
BspLI GGNNCC 2 cut(s) 499, 741
BspPI GGATC 4 cut(s) 734, 747, 885, 893
BspTNI GGTCTC 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 100, 845
BssMI GATC 5 cut(s) 248, 324, 739, 877, 885
BssNI GRCGYC 1 cut(s) 1116
BssT1I CCWWGG 1 cut(s) 845
Bst2UI CCWGG 2 cut(s) 101, 170
Bst4CI ACNGT 5 cut(s) 332, 829, 859, 1005, 1148
BstACI GRCGYC 1 cut(s) 1116
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 620, 624
BstDEI CTNAG 2 cut(s) 269, 351
BstDSI CCRYGG 1 cut(s) 845
BstF5I GGATG 4 cut(s) 487, 544, 855, 1087
BstFNI CGCG 2 cut(s) 48, 454
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 5 cut(s) 251, 327, 742, 880, 888
BstMAI GTCTC 2 cut(s) 72, 211
BstMBI GATC 5 cut(s) 248, 324, 739, 877, 885
BstMWI GCNNNNNNNGC 4 cut(s) 527, 599, 755, 764
BstNI CCWGG 2 cut(s) 101, 170
BstSCI CCNGG 2 cut(s) 99, 168
BstSLI GKGCMC 1 cut(s) 643
BstUI CGCG 2 cut(s) 48, 454
BstV1I GCAGC 5 cut(s) 139, 577, 614, 643, 784
BstX2I RGATCY 2 cut(s) 248, 739
BstYI RGATCY 2 cut(s) 248, 739
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 845
BtgZI GCGATG 1 cut(s) 782
BtsCI GGATG 4 cut(s) 487, 544, 855, 1087
BtsI GCAGTG 2 cut(s) 504, 643
BtsIMutI CAGTG 6 cut(s) 277, 337, 504, 643, 943, 1035
Cac8I GCNNGC 2 cut(s) 620, 624
CaiI CAGNNNCTG 2 cut(s) 350, 473
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 2 cut(s) 377, 812
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 5 cut(s) 24, 52, 567, 614, 1149
CviAII CATG 5 cut(s) 494, 764, 846, 874, 1073
CviJI RGCY 6 cut(s) 123, 343, 357, 442, 546, 656
CviKI_1 RGCY 6 cut(s) 123, 343, 357, 442, 546, 656
CviQI GTAC 5 cut(s) 24, 52, 567, 614, 1149
DdeI CTNAG 2 cut(s) 269, 351
DpnI GATC 5 cut(s) 250, 326, 741, 879, 887
DpnII GATC 5 cut(s) 248, 324, 739, 877, 885
DraI TTTAAA 1 cut(s) 361
EaeI YGGCCR 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 845
Eco31I GGTCTC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 377, 812
EcoRI GAATTC 1 cut(s) 1024
EcoRII CCWGG 2 cut(s) 99, 168
EcoT14I CCWWGG 1 cut(s) 845
ErhI CCWWGG 1 cut(s) 845
FaeI CATG 5 cut(s) 497, 767, 849, 877, 1076
FaqI GGGAC 3 cut(s) 269, 276, 279
FatI CATG 5 cut(s) 493, 763, 845, 873, 1072
FauI CCCGC 1 cut(s) 793
FbaI TGATCA 1 cut(s) 324
FblI GTMKAC 2 cut(s) 14, 687
Fnu4HI GCNGC 6 cut(s) 153, 415, 591, 603, 657, 798
FokI GGATG 4 cut(s) 494, 551, 862, 1094
Fsp4HI GCNGC 6 cut(s) 153, 415, 591, 603, 657, 798
FspBI CTAG 5 cut(s) 86, 557, 905, 923, 1133
GlaI GCGC 1 cut(s) 453
GluI GCNGC 6 cut(s) 153, 415, 591, 603, 657, 798
HaeIII GGCC 1 cut(s) 343
HgaI GACGC 1 cut(s) 1124
HhaI GCGC 1 cut(s) 454
Hin1I GRCGYC 1 cut(s) 1116
Hin1II CATG 5 cut(s) 497, 767, 849, 877, 1076
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 4 cut(s) 267, 347, 470, 684
HphI GGTGA 1 cut(s) 48
Hpy166II GTNNAC 6 cut(s) 15, 52, 641, 688, 1033, 1130
Hpy188I TCNGA 3 cut(s) 219, 352, 387
Hpy188III TCNNGA 1 cut(s) 1142
Hpy8I GTNNAC 6 cut(s) 15, 52, 641, 688, 1033, 1130
HpyAV CCTTC 6 cut(s) 190, 362, 404, 557, 825, 1153
HpyCH4III ACNGT 5 cut(s) 332, 829, 859, 1005, 1148
HpyCH4IV ACGT 1 cut(s) 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 527, 599, 755, 764
HpyF3I CTNAG 2 cut(s) 269, 351
HpySE526I ACGT 1 cut(s) 650
Hsp92I GRCGYC 1 cut(s) 1116
Hsp92II CATG 5 cut(s) 497, 767, 849, 877, 1076
HspAI GCGC 1 cut(s) 452
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 5 cut(s) 248, 324, 739, 877, 885
Lsp1109I GCAGC 5 cut(s) 139, 577, 614, 643, 784
LweI GCATC 4 cut(s) 298, 658, 840, 1072
MaeI CTAG 5 cut(s) 86, 557, 905, 923, 1133
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 1 cut(s) 938
MalI GATC 5 cut(s) 250, 326, 741, 879, 887
MboI GATC 5 cut(s) 248, 324, 739, 877, 885
MboII GAAGA 2 cut(s) 708, 784
MfeI CAATTG 2 cut(s) 705, 750
MflI RGATCY 2 cut(s) 248, 739
MhlI GDGCHC 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 343
MlyI GAGTC 4 cut(s) 261, 356, 479, 693
MnlI CCTC 9 cut(s) 304, 390, 393, 520, 569, 572, 730, 956, 1074
Mox20I TGGCCA 1 cut(s) 343
MroXI GAANNNNTTC 2 cut(s) 109, 1037
MscI TGGCCA 1 cut(s) 343
MseI TTAA 3 cut(s) 78, 360, 1046
MslI CAYNNNNRTG 1 cut(s) 631
Msp20I TGGCCA 1 cut(s) 343
MspR9I CCNGG 2 cut(s) 101, 170
MunI CAATTG 2 cut(s) 705, 750
MvaI CCWGG 2 cut(s) 101, 170
MvnI CGCG 2 cut(s) 48, 454
MwoI GCNNNNNNNGC 4 cut(s) 527, 599, 755, 764
NcoI CCATGG 1 cut(s) 845
NdeII GATC 5 cut(s) 248, 324, 739, 877, 885
NlaIII CATG 5 cut(s) 497, 767, 849, 877, 1076
NlaIV GGNNCC 2 cut(s) 499, 741
NmuCI GTSAC 1 cut(s) 938
OliI CACNNNNGTG 1 cut(s) 631
PdmI GAANNNNTTC 2 cut(s) 109, 1037
PkrI GCNGC 6 cut(s) 154, 416, 592, 604, 658, 799
PleI GAGTC 4 cut(s) 261, 355, 478, 692
PpsI GAGTC 4 cut(s) 261, 355, 478, 692
Psp6I CCWGG 2 cut(s) 99, 168
PspGI CCWGG 2 cut(s) 99, 168
PspN4I GGNNCC 2 cut(s) 499, 741
PspPI GGNCC 2 cut(s) 377, 812
PstNI CAGNNNCTG 2 cut(s) 350, 473
PsuI RGATCY 2 cut(s) 248, 739
RsaI GTAC 5 cut(s) 25, 53, 568, 615, 1150
RsaNI GTAC 5 cut(s) 24, 52, 567, 614, 1149
RseI CAYNNNNRTG 1 cut(s) 631
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 3 cut(s) 78, 360, 1046
SatI GCNGC 6 cut(s) 153, 415, 591, 603, 657, 798
Sau3AI GATC 5 cut(s) 248, 324, 739, 877, 885
Sau96I GGNCC 2 cut(s) 377, 812
SchI GAGTC 4 cut(s) 261, 356, 479, 693
ScrFI CCNGG 2 cut(s) 101, 170
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 4 cut(s) 298, 658, 840, 1072
SinI GGWCC 2 cut(s) 377, 812
SmiMI CAYNNNNRTG 1 cut(s) 631
SpeI ACTAGT 1 cut(s) 922
SsiI CCGC 2 cut(s) 414, 800
SspMI CTAG 5 cut(s) 86, 557, 905, 923, 1133
StyD4I CCNGG 2 cut(s) 99, 168
StyI CCWWGG 1 cut(s) 845
TaaI ACNGT 5 cut(s) 332, 829, 859, 1005, 1148
TaiI ACGT 1 cut(s) 653
TaqI TCGA 2 cut(s) 286, 687
TatI WGTACW 2 cut(s) 51, 1148
TauI GCSGC 1 cut(s) 417
Tru1I TTAA 3 cut(s) 78, 360, 1046
Tru9I TTAA 3 cut(s) 78, 360, 1046
TscAI CASTG 6 cut(s) 277, 337, 511, 643, 943, 1035
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 5 cut(s) 152, 590, 602, 656, 797
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 6 cut(s) 209, 381, 498, 752, 785, 1061
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 6 cut(s) 277, 337, 511, 643, 943, 1035
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 2 cut(s) 377, 812
XapI RAATTY 5 cut(s) 278, 336, 421, 724, 1024
XmiI GTMKAC 2 cut(s) 14, 687
XmnI GAANNNNTTC 2 cut(s) 109, 1037
XspI CTAG 5 cut(s) 86, 557, 905, 923, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.