RchiOBHm_Chr1g0323291

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000257
Physical Location & Seq
Reverse (-)
10831942 .. 10834158
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55320

Sequence Viewer

Length: 2217 bp
ATGGCTAAGCATGGAGCATTCCTATTCTCTTATGCTTTCTCTACACTCGTACTTACTATGAGCTTATTGTGCAATGCCATTGATCATGAAGATAGAAAGGTTCATATAGTGTATCTGGGATCACTTCCGGATGATGAGGAGTACTCGCCAAGGTCTCATCACCTCGATATACTGCAAAGAGTTGTTGATGGTAGTTCTGCTGAAAATTGCTTGATAAGGAGCTACGAAAGGAGCTTCAATGGATTTGCTGCAAAGCTCAGTGATCATGAAAAGGAAAAGACTGCTAACATGGAGGAGGTGGTCTCTGTCTTTCCTAGCAAAACCTTCCAACTTCAAACGACGAGGTCATGGGACTTTATGGGTCTCAAGCTGACTAGTCCTCGAAAAGCCATTGCTGAGAGTAATGTTATTGTTGCTGTGATTGACTCTGGAATTGACCCTGATTCGGAGAGCTTTAGAGATGAAGGTTTTGGTGCTGCTCCTATGAAGTGGAAAGGTGTTTGTGAAGGTGGCGAAAATTTCAGCTGCAACAACAAGATAATTGGAGCTCGTTTTTACGACCCAGCAGAGTCTGCAAGGGATGAAGACGGCCATGGAACCCACACTGCCTCAACGGCAGCAGGGAATGTGGTAAAGGATGTAAGCTTTTACGGATTAGAACCAGGTACAGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTCTGTGGGGCTATGAGCAGGTGCTCTTCAGAGGCTATATTGGCTGCTTTTGACGATGCAATCGCTGATGGAGTTGACATTGTGTCAGTCTCACTTGCAAGTGGAAGGCCCCTTCCTTTTGATAATGATGTTATAGCAGTTGGTTCTTATCATGCAATGAAGAAAGGGATATTGACATCAAACTCTGCAGGCAACCATGGTCCTGGAGCTGGTACTGTAACAAGTGTAGCGCCGTGGATGCTTGCAGTTGCAGCAAGTAGCAAAGACCGTCGGATCATTGACAAGGCCGTTCTTGGAAGTAGGACACTAGTAGGGGCTTCAGTGAACTCTTTCATATTGAAAGGTAGTTTTCCATTGATATATGGAAAAGATGCGTCAAGTAAATGCCCCGAATACTATGCTAGGAGTTGTGAAGCTGGTTGCCTAGACAGTAATTCAGTTCAAAACAAGATCGTCCTATGTGACCAGCCCGCTGGAGTATATGAGGCTGATCGAGCTGGTGCACGAGGCGCAATTTTCTACAATCCCATACCTGATTATGCTTCTGTTTTCCCAATGCCTGCTGTGGGTTTGAGATCCAAAGACTATAATGTGGCCATATCTTATATGAACTCCACGAATGATCCTCAAGCAAGCATACCAAAGAGTGAAAACATAAAAGATCCAGCTGCACCTGAAGTTGTTTCTTTCTCTTCACGTGGACCTAATCAAATTTTACCTGAAATTATTAAGCCAGATGTAACTGGCCCGGGAGTGACCATTATAGCTGCATATCCGAGTGATGTTCCTGTCACAAATGGCTCTAGAGACACAAGACGAGTCAAGTATAATATACTATCCGGAACCTCCATGTCTTGCCCCCACGCTACTGGTGTAGCTGCATATGTTAAAGAGTTCCACCCTGATTGGTCTCCAGCAGCCATTAAATCTGCTCTTATGACTACAGCTTGGCCTATGAATGATACATCCAACAGCTCATCCCCTGGTGAATTTGCATATGGATCCGGTCATATCAATCCTCTAAAAGCTATTGACCCAGGGCTTGTGTATGATGCTTCTGCAGAGGATTACATTAAGTTACTCTGTTTGGTCATGGACGAGGCGAGAGTTAGACTTATATCAGGAGATAACAGCACTTGTCCTACAGCTTCTAAACAAGGATCGGCAAAGGATCTCAACTACCCTTCAATATCAGCTGTTGTTACACCAATGACACCTTTTACGGTGACAATACGTAGAAGAGTTACAAATGTTGGCCTTGCAAATTCCACTTACAAGGCCGAAATCATGCCAAATTCTCAAGTTGACATCAAAGTGGAGCCTCAAGTTCTTTCCTTCAAGTCCATGAACGAGGAGAAGACTTTCAATCTGACCATTGCTGGAAGAGGGTTGCCAGATGGATCACATATGTCCTCGTCCTTGGTTTGGTCCGACGGAACTCATAAGGTTAAAAGTCCAGTTCTAGTGCAAAGCTTATCAGCAGCAGTAGCATCACGATTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

738

Amino Acids

78.88

Weight (kDa)

6.09

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 112 2.4e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 564 3.9e-44 Subtilase family
fn3_6 PF17766 628 - 726 6.2e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 720
Acc36I ACCTGC 1 cut(s) 720
AccIII TCCGGA 2 cut(s) 127, 1550
AciI CCGC 1 cut(s) 1182
AcoI YGGCCR 2 cut(s) 589, 1305
AcsI RAATTY 5 cut(s) 517, 1422, 1700, 1975, 2005
AcuI CTGAAG 3 cut(s) 723, 1014, 1407
AcvI CACGTG 1 cut(s) 1409
AfaI GTAC 4 cut(s) 51, 143, 667, 925
AfiI CCNNNNNNNGG 4 cut(s) 445, 920, 1277, 2136
AgsI TTSAA 7 cut(s) 238, 335, 1051, 1154, 1899, 2050, 2077
AhdI GACNNNNNGTC 1 cut(s) 793
AhlI ACTAGT 2 cut(s) 374, 1018
AjnI CCWGG 4 cut(s) 661, 913, 1693, 1747
Alw21I GWGCWC 3 cut(s) 550, 737, 1216
Alw26I GTCTC 6 cut(s) 159, 307, 368, 805, 1512, 1626
Alw44I GTGCAC 1 cut(s) 1212
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 1459
Aor13HI TCCGGA 2 cut(s) 127, 1550
AoxI GGCC 8 cut(s) 589, 818, 996, 1305, 1456, 1661, 1966, 1989
ApaLI GTGCAC 1 cut(s) 1212
ApoI RAATTY 5 cut(s) 517, 1422, 1700, 1975, 2005
Asp700I GAANNNNTTC 2 cut(s) 1040, 2072
AspLEI GCGC 2 cut(s) 943, 1223
AspS9I GGNCC 5 cut(s) 819, 911, 1412, 1457, 2139
AsuC2I CCSGG 2 cut(s) 1460, 1461
AsuHPI GGTGA 3 cut(s) 152, 1709, 1948
AvaI CYCGRG 1 cut(s) 1459
AvaII GGWCC 3 cut(s) 911, 1412, 2139
BaeGI GKGCMC 1 cut(s) 1216
BalI TGGCCA 1 cut(s) 1307
BamHI GGATCC 1 cut(s) 1712
BanII GRGCYC 1 cut(s) 550
BauI CACGAG 1 cut(s) 1215
BbrPI CACGTG 1 cut(s) 1409
BbsI GAAGAC 2 cut(s) 591, 2075
Bbv12I GWGCWC 3 cut(s) 550, 737, 1216
BbvCI CCTCAGC 1 cut(s) 688
BccI CCATC 3 cut(s) 182, 773, 2102
BceAI ACGGC 4 cut(s) 604, 630, 928, 983
BcgI CGANNNNNNTGC 2 cut(s) 1091, 1125
BciT130I CCWGG 4 cut(s) 663, 915, 1695, 1749
BclI TGATCA 2 cut(s) 82, 262
BcnI CCSGG 2 cut(s) 1460, 1461
BcoDI GTCTC 6 cut(s) 159, 307, 368, 805, 1512, 1626
BcuI ACTAGT 2 cut(s) 374, 1018
BfaI CTAG 7 cut(s) 315, 375, 1019, 1113, 1136, 1515, 2174
BfmI CTRYAG 4 cut(s) 897, 1653, 1770, 1854
BfoI RGCGCY 1 cut(s) 944
BfuAI ACCTGC 1 cut(s) 720
BglI GCCNNNNNGGC 1 cut(s) 614
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 143
Bme1390I CCNGG 6 cut(s) 663, 915, 1460, 1461, 1695, 1749
Bme18I GGWCC 3 cut(s) 911, 1412, 2139
BmeRI GACNNNNNGTC 1 cut(s) 793
BmeT110I CYCGRG 1 cut(s) 1459
BmgT120I GGNCC 5 cut(s) 819, 911, 1412, 1457, 2139
BmiI GGNNCC 6 cut(s) 598, 684, 821, 1555, 1714, 2031
BmrFI CCNGG 6 cut(s) 663, 915, 1460, 1461, 1695, 1749
BmsI GCATC 5 cut(s) 757, 939, 1072, 1753, 2210
BpiI GAAGAC 2 cut(s) 591, 2075
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 287, 319
BpmI CTGGAG 3 cut(s) 936, 1206, 1608
Bpu10I CCTNAGC 1 cut(s) 688
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 350, 1323, 1995, 2019
BpuMI CCSGG 2 cut(s) 1460, 1461
BsaAI YACGTR 2 cut(s) 1409, 1946
BsaI GGTCTC 4 cut(s) 159, 307, 368, 1626
BsaJI CCNNGG 9 cut(s) 149, 592, 907, 944, 1459, 1693, 1747, 1748, 2130
BsaWI WCCGGW 3 cut(s) 127, 1550, 1715
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 920, 1277, 2136
Bse1I ACTGG 3 cut(s) 1459, 1585, 2168
Bse3DI GCAATG 4 cut(s) 79, 390, 873, 2085
BseAI TCCGGA 2 cut(s) 127, 1550
BseBI CCWGG 4 cut(s) 663, 915, 1695, 1749
BseDI CCNNGG 9 cut(s) 149, 592, 907, 944, 1459, 1693, 1747, 1748, 2130
BseGI GGATG 6 cut(s) 136, 586, 643, 954, 1676, 1688
BseLI CCNNNNNNNGG 4 cut(s) 445, 920, 1277, 2136
BseMI GCAATG 4 cut(s) 79, 390, 873, 2085
BseMII CTCAG 3 cut(s) 271, 387, 702
BseNI ACTGG 3 cut(s) 1459, 1585, 2168
BseRI GAGGAG 4 cut(s) 152, 308, 690, 2078
BseSI GKGCMC 1 cut(s) 1216
BseYI CCCAGC 1 cut(s) 562
BsgI GTGCAG 1 cut(s) 1365
BshFI GGCC 8 cut(s) 591, 820, 998, 1307, 1458, 1663, 1968, 1991
BsiHKAI GWGCWC 3 cut(s) 550, 737, 1216
BsiHKCI CYCGRG 1 cut(s) 1459
BsiSI CCGG 4 cut(s) 128, 1460, 1551, 1716
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 4 cut(s) 445, 920, 1277, 2136
BsmAI GTCTC 6 cut(s) 159, 307, 368, 805, 1512, 1626
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 8 cut(s) 591, 820, 998, 1307, 1458, 1663, 1968, 1991
Bso31I GGTCTC 4 cut(s) 159, 307, 368, 1626
BsoBI CYCGRG 1 cut(s) 1459
Bsp1286I GDGCHC 3 cut(s) 550, 737, 1216
Bsp13I TCCGGA 2 cut(s) 127, 1550
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 592, 907
BspACI CCGC 1 cut(s) 1182
BspANI GGCC 8 cut(s) 591, 820, 998, 1307, 1458, 1663, 1968, 1991
BspCNI CTCAG 3 cut(s) 270, 388, 701
BspEI TCCGGA 2 cut(s) 127, 1550
BspHI TCATGA 2 cut(s) 85, 265
BspLI GGNNCC 6 cut(s) 598, 684, 821, 1555, 1714, 2031
BspMAI CTGCAG 2 cut(s) 901, 1774
BspMI ACCTGC 1 cut(s) 720
BspQI GCTCTTC 1 cut(s) 742
BspTNI GGTCTC 4 cut(s) 159, 307, 368, 1626
BsrDI GCAATG 4 cut(s) 79, 390, 873, 2085
BsrI ACTGG 3 cut(s) 1459, 1585, 2168
BssECI CCNNGG 9 cut(s) 149, 592, 907, 944, 1459, 1693, 1747, 1748, 2130
BssSI CACGAG 1 cut(s) 1215
BssT1I CCWWGG 4 cut(s) 149, 592, 907, 2130
Bst2BI CACGAG 1 cut(s) 1215
Bst2UI CCWGG 4 cut(s) 663, 915, 1695, 1749
Bst4CI ACNGT 4 cut(s) 928, 980, 1142, 1936
Bst6I CTCTTC 4 cut(s) 742, 1408, 1945, 2089
BstAPI GCANNNNNTGC 1 cut(s) 572
BstBAI YACGTR 2 cut(s) 1409, 1946
BstC8I GCNNGC 5 cut(s) 901, 954, 1182, 1272, 1345
BstDEI CTNAG 4 cut(s) 6, 257, 396, 688
BstDSI CCRYGG 3 cut(s) 592, 907, 944
BstF5I GGATG 6 cut(s) 136, 586, 643, 954, 1676, 1688
BstH2I RGCGCY 1 cut(s) 944
BstHHI GCGC 2 cut(s) 943, 1223
BstMAI GTCTC 6 cut(s) 159, 307, 368, 805, 1512, 1626
BstNI CCWGG 4 cut(s) 663, 915, 1695, 1749
BstSCI CCNGG 6 cut(s) 661, 913, 1458, 1459, 1693, 1747
BstSFI CTRYAG 4 cut(s) 897, 1653, 1770, 1854
BstSLI GKGCMC 1 cut(s) 1216
BstSNI TACGTA 1 cut(s) 1946
BstV2I GAAGAC 2 cut(s) 591, 2075
BstX2I RGATCY 4 cut(s) 1286, 1372, 1712, 1882
BstXI CCANNNNNNTGG 3 cut(s) 914, 1184, 1580
BstYI RGATCY 4 cut(s) 1286, 1372, 1712, 1882
BsuRI GGCC 8 cut(s) 591, 820, 998, 1307, 1458, 1663, 1968, 1991
BtgI CCRYGG 3 cut(s) 592, 907, 944
BtsCI GGATG 6 cut(s) 136, 586, 643, 954, 1676, 1688
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 265, 603, 1038
BveI ACCTGC 1 cut(s) 720
Cac8I GCNNGC 5 cut(s) 901, 954, 1182, 1272, 1345
CaiI CAGNNNCTG 1 cut(s) 572
CciI TCATGA 2 cut(s) 85, 265
CfoI GCGC 2 cut(s) 943, 1223
Cfr13I GGNCC 5 cut(s) 819, 911, 1412, 1457, 2139
Cfr9I CCCGGG 1 cut(s) 1459
CseI GACGC 1 cut(s) 1074
CsiI ACCWGGT 1 cut(s) 661
Csp6I GTAC 4 cut(s) 50, 142, 666, 924
CspCI CAANNNNNGTGG 2 cut(s) 1598, 1633
CviQI GTAC 4 cut(s) 50, 142, 666, 924
DdeI CTNAG 4 cut(s) 6, 257, 396, 688
DriI GACNNNNNGTC 1 cut(s) 793
EaeI YGGCCR 2 cut(s) 589, 1305
Eam1104I CTCTTC 4 cut(s) 742, 1408, 1945, 2089
Eam1105I GACNNNNNGTC 1 cut(s) 793
EarI CTCTTC 4 cut(s) 742, 1408, 1945, 2089
Ecl136II GAGCTC 1 cut(s) 548
Eco105I TACGTA 1 cut(s) 1946
Eco130I CCWWGG 4 cut(s) 149, 592, 907, 2130
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 4 cut(s) 159, 307, 368, 1626
Eco47I GGWCC 3 cut(s) 911, 1412, 2139
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 3 cut(s) 723, 1014, 1407
Eco72I CACGTG 1 cut(s) 1409
Eco88I CYCGRG 1 cut(s) 1459
EcoICRI GAGCTC 1 cut(s) 548
EcoO109I RGGNCCY 1 cut(s) 819
EcoRII CCWGG 4 cut(s) 661, 913, 1693, 1747
EcoT14I CCWWGG 4 cut(s) 149, 592, 907, 2130
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 4 cut(s) 149, 592, 907, 2130
FaqI GGGAC 1 cut(s) 365
FauI CCCGC 1 cut(s) 1189
FauNDI CATATG 3 cut(s) 1594, 1708, 2118
FbaI TGATCA 2 cut(s) 82, 262
FokI GGATG 6 cut(s) 143, 593, 650, 961, 1663, 1675
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 7 cut(s) 315, 375, 1019, 1113, 1136, 1515, 2174
GlaI GCGC 2 cut(s) 942, 1222
GsaI CCCAGC 1 cut(s) 566
GsuI CTGGAG 3 cut(s) 936, 1206, 1608
HaeII RGCGCY 1 cut(s) 944
HaeIII GGCC 8 cut(s) 591, 820, 998, 1307, 1458, 1663, 1968, 1991
HapII CCGG 4 cut(s) 128, 1460, 1551, 1716
HgaI GACGC 1 cut(s) 1074
HhaI GCGC 2 cut(s) 943, 1223
Hin6I GCGC 2 cut(s) 941, 1221
HinP1I GCGC 2 cut(s) 941, 1221
HincII GTYRAC 2 cut(s) 787, 2017
HindII GTYRAC 2 cut(s) 787, 2017
HindIII AAGCTT 2 cut(s) 643, 2182
HinfI GANTC 4 cut(s) 425, 443, 569, 1530
HpaII CCGG 4 cut(s) 128, 1460, 1551, 1716
HphI GGTGA 3 cut(s) 152, 1709, 1948
Hpy166II GTNNAC 5 cut(s) 787, 1036, 1214, 1412, 2017
Hpy188I TCNGA 6 cut(s) 448, 742, 984, 1488, 2082, 2143
Hpy188III TCNNGA 8 cut(s) 86, 128, 266, 429, 1515, 1551, 1833, 2205
Hpy8I GTNNAC 5 cut(s) 787, 1036, 1214, 1412, 2017
Hpy99I CGWCG 3 cut(s) 343, 984, 2147
HpyAV CCTTC 7 cut(s) 334, 458, 500, 810, 833, 1905, 2056
HpyCH4III ACNGT 4 cut(s) 928, 980, 1142, 1936
HpyCH4IV ACGT 2 cut(s) 1408, 1945
HpyF3I CTNAG 4 cut(s) 6, 257, 396, 688
HpySE526I ACGT 2 cut(s) 1408, 1945
HspAI GCGC 2 cut(s) 941, 1221
Kpn2I TCCGGA 2 cut(s) 127, 1550
Ksp22I TGATCA 2 cut(s) 82, 262
LguI GCTCTTC 1 cut(s) 742
LmnI GCTCC 7 cut(s) 14, 219, 231, 484, 545, 917, 2029
LweI GCATC 5 cut(s) 757, 939, 1072, 1753, 2210
MabI ACCWGGT 1 cut(s) 661
MaeI CTAG 7 cut(s) 315, 375, 1019, 1113, 1136, 1515, 2174
MaeII ACGT 2 cut(s) 1408, 1945
MaeIII GTNAC 9 cut(s) 928, 1172, 1450, 1465, 1501, 1788, 1912, 1936, 1954
MboII GAAGA 8 cut(s) 101, 596, 729, 883, 1395, 1962, 2080, 2106
MflI RGATCY 4 cut(s) 1286, 1372, 1712, 1882
MhlI GDGCHC 3 cut(s) 550, 737, 1216
MlsI TGGCCA 1 cut(s) 1307
MluNI TGGCCA 1 cut(s) 1307
MlyI GAGTC 3 cut(s) 419, 578, 1539
MmeI TCCRAC 4 cut(s) 352, 962, 1704, 2166
Mox20I TGGCCA 1 cut(s) 1307
MroI TCCGGA 2 cut(s) 127, 1550
MroXI GAANNNNTTC 2 cut(s) 1040, 2072
MscI TGGCCA 1 cut(s) 1307
MseI TTAA 6 cut(s) 1440, 1599, 1635, 1785, 2160, 2215
MslI CAYNNNNRTG 1 cut(s) 2024
Msp20I TGGCCA 1 cut(s) 1307
MspA1I CMGCKG 4 cut(s) 525, 1184, 1379, 1907
MspI CCGG 4 cut(s) 128, 1460, 1551, 1716
MspR9I CCNGG 6 cut(s) 663, 915, 1460, 1461, 1695, 1749
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 4 cut(s) 663, 915, 1695, 1749
NciI CCSGG 2 cut(s) 1460, 1461
NcoI CCATGG 2 cut(s) 592, 907
NdeI CATATG 3 cut(s) 1594, 1708, 2118
NlaIV GGNNCC 6 cut(s) 598, 684, 821, 1555, 1714, 2031
NmuCI GTSAC 4 cut(s) 1172, 1465, 1501, 1936
PagI TCATGA 2 cut(s) 85, 265
PaqCI CACCTGC 1 cut(s) 720
PasI CCCWGGG 1 cut(s) 1748
PciSI GCTCTTC 1 cut(s) 742
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 2 cut(s) 1040, 2072
PfeI GAWTC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 913
PleI GAGTC 3 cut(s) 419, 577, 1538
PmaCI CACGTG 1 cut(s) 1409
PmlI CACGTG 1 cut(s) 1409
PpsI GAGTC 3 cut(s) 419, 577, 1538
Ppu21I YACGTR 2 cut(s) 1409, 1946
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 4 cut(s) 661, 913, 1693, 1747
PspCI CACGTG 1 cut(s) 1409
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 4 cut(s) 661, 913, 1693, 1747
PspN4I GGNNCC 6 cut(s) 598, 684, 821, 1555, 1714, 2031
PspPI GGNCC 5 cut(s) 819, 911, 1412, 1457, 2139
PstI CTGCAG 2 cut(s) 901, 1774
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 4 cut(s) 1286, 1372, 1712, 1882
PsyI GACNNNGTC 1 cut(s) 343
PvuII CAGCTG 3 cut(s) 525, 1379, 1907
RsaI GTAC 4 cut(s) 51, 143, 667, 925
RsaNI GTAC 4 cut(s) 50, 142, 666, 924
RseI CAYNNNNRTG 1 cut(s) 2024
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 1 cut(s) 742
SaqAI TTAA 6 cut(s) 1440, 1599, 1635, 1785, 2160, 2215
Sau96I GGNCC 5 cut(s) 819, 911, 1412, 1457, 2139
ScaI AGTACT 1 cut(s) 143
SchI GAGTC 3 cut(s) 419, 578, 1539
ScrFI CCNGG 6 cut(s) 663, 915, 1460, 1461, 1695, 1749
SduI GDGCHC 3 cut(s) 550, 737, 1216
SexAI ACCWGGT 1 cut(s) 661
SfaNI GCATC 5 cut(s) 757, 939, 1072, 1753, 2210
SfcI CTRYAG 4 cut(s) 897, 1653, 1770, 1854
SinI GGWCC 3 cut(s) 911, 1412, 2139
SmaI CCCGGG 1 cut(s) 1461
SmiMI CAYNNNNRTG 1 cut(s) 2024
SmlI CTYRAG 4 cut(s) 365, 1338, 2010, 2034
SmoI CTYRAG 4 cut(s) 365, 1338, 2010, 2034
SnaBI TACGTA 1 cut(s) 1946
SpeI ACTAGT 2 cut(s) 374, 1018
SsiI CCGC 1 cut(s) 1182
SspMI CTAG 7 cut(s) 315, 375, 1019, 1113, 1136, 1515, 2174
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 6 cut(s) 661, 913, 1458, 1459, 1693, 1747
StyI CCWWGG 4 cut(s) 149, 592, 907, 2130
TaaI ACNGT 4 cut(s) 928, 980, 1142, 1936
TaiI ACGT 2 cut(s) 1411, 1948
TaqI TCGA 3 cut(s) 165, 382, 1204
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 6 cut(s) 1440, 1599, 1635, 1785, 2160, 2215
Tru9I TTAA 6 cut(s) 1440, 1599, 1635, 1785, 2160, 2215
TscAI CASTG 3 cut(s) 265, 610, 1038
TseFI GTSAC 4 cut(s) 1172, 1465, 1501, 1936
Tsp45I GTSAC 4 cut(s) 1172, 1465, 1501, 1936
TspGWI ACGGA 2 cut(s) 666, 2160
TspMI CCCGGG 1 cut(s) 1459
TspRI CASTG 3 cut(s) 265, 610, 1038
Tth111I GACNNNGTC 1 cut(s) 343
VneI GTGCAC 1 cut(s) 1212
VpaK11BI GGWCC 3 cut(s) 911, 1412, 2139
XapI RAATTY 5 cut(s) 517, 1422, 1700, 1975, 2005
XbaI TCTAGA 1 cut(s) 1514
XmaI CCCGGG 1 cut(s) 1459
XmnI GAANNNNTTC 2 cut(s) 1040, 2072
XspI CTAG 7 cut(s) 315, 375, 1019, 1113, 1136, 1515, 2174
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.