RLG00000030004

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000257
Physical Location & Seq
Reverse (-)
53198255 .. 53200462
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030004

Sequence Viewer

Length: 2208 bp
ATGGCTAAGCATGGAGCTATCCTATTTTCTTATGTTTTCCCCAGTCTTATACTAACCTTGAACTTCTTCATGTGCAAAGCCATCGATGAAAACAGAAAGGTCCATATTGTGTACCTGGGTTCACTTCCTGATAATGAGGTGTACTCACCATTGTCTCACCACCTTGGTCTACTTGAAAAAGTTGTCGAGAGCAACTCCGCTGCAAGTTTCTTGACAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCAAAGCTCACTGACCGCGAAAGGGAAAGGCTTGCTAATATGAAGGAAGTAGTCTCTGTCTTTCCAAACAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCAATGAGAAAACCAAACGGAATGCTAGTGTTGAGAGTGATACCATTATTGGTGTCATTGATTCTGGAATCTGGCCTGAATCAGAGAGCTTTAAAGATGATGGTTTTGGTCTTCCTCCCAAGAAGTGGAAAGGTGCTTGTGAAGGTGGCAAAAATTTCACTTGCAACAAGAAGCTGATTGGAGCTCGGTTTTACGCTCCATACTTAAAGGATGCAAGGGATCAAATAGGCCATGGAACCCACACAGCCTCAACTGCAGCAGGGAACGTTGTAAAAGATGTGAGCTTTTATGGACTAGCACAAGGTACTGCAAGAGGAGGGGTTCCCTCAGCTAGAATTGCTGCATATAAAGTCTGTGATGCTTTTGGGTGTCCTACAGAGAATATCTTGGCTGCTTTTGACGATGCTATTGCCGATGGAGTTGACATCATTACAATTTCAATAGGAAGCGTGCAAGCATCTTTATTACAGTATGATCCTATTGCTATTGGTGCTTTTCATGCAATGACAAAGGGGATACTTACATCACATTCTGCAGGCAACAGTGGTCCTGATGATTCAAGTGTTGCAAGTGTAGCACCATGGATACTTGCAGTTGCAGCAAGTAGTACTGATCGTAGAATCATTGACAACGTTGTTCTCGAAAATGGGACGACATTGGTTGGGGCTACAGTGAACACTTTCACATTAAATGGGACAAGTTTTCCATTGATATATGGAAAAGATGCTTCAAGTCAATGCTCTGAGTCCAAAGCTGAGAGTTGTACAGAAGGCTGCTTAGACAGTGGTTTAGTTAAGGGAAAGGTTGTGTTATGTGATGAGCCCTTCGGAGTTTATGAAGCCAATAGTTCTGGAGCAGTAGGCTCGATTCTGAGATTTGATTGGGATGATGTTTCTCCAATTGTACCCTTTCCTGCAACAGGTTTCACCTACGAAGAGCATAGTCTGATCAAGTTGTACATGAACTCCACGAAAGATGCTCGAGTAAACATATTAAAAAGTGAAGTCAAAAGGGATACCACTGCACCTGTCGTTGCTTCCTTCTCTTCACGTGGACCAAATTCAATTCTACCAGAAATTATCAAGCCAGATATAAGCGCCCCAGGTGTAACTATTTTGGCTGCCTTTTCACCTGCTGGTGTACTTACAAGGAGTCTTGAAGACACAAGGCATGTAAATTACAGTATATTATCTGGAACCTCTATGTCCTGCCCGCACGTGGCCGGTGCTGCTGCATATGTTAAAGAATTCCATCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTATTCCTATGAATGTTACTGATAATAGCAAAGCCGCTGGTGAATTTGCATATGGATCCGGACATATCAATCCTGTTAAAGCTATAGACCCGGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACATAAAGTTGCTCTGCTTGGTCTTAAACCAGACCGATGTTAGACTTATATCAGGAGATAACAACACTTGCTCTACAGGCTCCGACAAAGGATCTTTACAAGATCACAATTACCCTTCACTAGCAGCCGTTGTCGTACCAAACAAATCTTTTTCGTTAAATTTTCACAGAACAGTGAAGAATGTTGGCCTTGCAAACTCCACTTACAATGCTACAGTATTCGCCAACTCTACTGAAGTTGACATCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCATTGGATGAGGAGAAGACTTTTGATGTGACCGTTGCTGGAAAAGGTTTGTCCGATGAAGCACAATCACATGTGTCTGCATCGTTGGTGTGGTCTGATGGAATTCATAGCGTTAGAAGTCCAATTGTTATCTACAAAAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

736

Amino Acids

78.81

Weight (kDa)

6.16

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 110 1.4e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 575 3.6e-44 Subtilase family
fn3_6 PF17766 630 - 731 2.6e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1504
Acc36I ACCTGC 1 cut(s) 1504
AccB1I GGYRCC 1 cut(s) 2038
AccB7I CCANNNNNTGG 1 cut(s) 489
AccI GTMKAC 1 cut(s) 169
AccII CGCG 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 1721
AciI CCGC 4 cut(s) 198, 265, 1577, 1698
AclI AACGTT 2 cut(s) 630, 996
AclWI GGATC 5 cut(s) 591, 833, 1713, 1726, 1885
AcoI YGGCCR 1 cut(s) 1584
AcsI RAATTY 7 cut(s) 321, 517, 1423, 1610, 1706, 1945, 2163
AcuI CTGAAG 1 cut(s) 2040
AcvI CACGTG 2 cut(s) 1415, 1582
AfaI GTAC 9 cut(s) 113, 143, 670, 973, 1129, 1269, 1322, 1506, 1923
AfiI CCNNNNNNNGG 4 cut(s) 271, 489, 1283, 1582
AflIII ACRYGT 1 cut(s) 2131
AjnI CCWGG 2 cut(s) 114, 1465
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 3 cut(s) 159, 307, 1632
AlwI GGATC 5 cut(s) 591, 833, 1713, 1726, 1885
Ama87I CYCGRG 2 cut(s) 1344, 1753
Aor13HI TCCGGA 1 cut(s) 1721
AoxI GGCC 4 cut(s) 437, 592, 1584, 1972
ApoI RAATTY 7 cut(s) 321, 517, 1423, 1610, 1706, 1945, 2163
Asp700I GAANNNNTTC 2 cut(s) 65, 1043
AspLEI GCGC 1 cut(s) 1463
AspS9I GGNCC 3 cut(s) 100, 911, 1418
AsuC2I CCSGG 2 cut(s) 1754, 1755
AsuHPI GGTGA 5 cut(s) 138, 149, 1282, 1485, 1715
AvaI CYCGRG 2 cut(s) 1344, 1753
AvaII GGWCC 3 cut(s) 100, 911, 1418
BamHI GGATCC 1 cut(s) 1718
BanI GGYRCC 1 cut(s) 2038
BanII GRGCYC 2 cut(s) 550, 1188
BbrPI CACGTG 2 cut(s) 1415, 1582
BbsI GAAGAC 4 cut(s) 467, 1530, 1788, 2084
Bbv12I GWGCWC 1 cut(s) 550
BbvCI CCTCAGC 1 cut(s) 691
BccI CCATC 7 cut(s) 89, 336, 458, 773, 1623, 1646, 2153
BceAI ACGGC 1 cut(s) 1898
BcgI CGANNNNNNTGC 6 cut(s) 64, 98, 755, 789, 1209, 1243
BciT130I CCWGG 2 cut(s) 116, 1467
BciVI GTATCC 3 cut(s) 873, 942, 1372
BclI TGATCA 1 cut(s) 1311
BcnI CCSGG 2 cut(s) 1754, 1755
BcoDI GTCTC 3 cut(s) 159, 307, 1632
BfaI CTAG 4 cut(s) 390, 659, 696, 1907
BfmI CTRYAG 8 cut(s) 618, 738, 897, 1032, 1659, 1746, 1860, 1998
BfoI RGCGCY 1 cut(s) 1464
BfuAI ACCTGC 1 cut(s) 1504
BfuI GTATCC 3 cut(s) 873, 942, 1372
BglII AGATCT 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 4 cut(s) 116, 1467, 1754, 1755
Bme18I GGWCC 3 cut(s) 100, 911, 1418
BmeT110I CYCGRG 2 cut(s) 1344, 1753
BmgT120I GGNCC 3 cut(s) 100, 911, 1418
BmiI GGNNCC 6 cut(s) 601, 687, 1561, 1720, 1867, 2040
BmrFI CCNGG 4 cut(s) 116, 1467, 1754, 1755
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 7 cut(s) 565, 712, 757, 830, 1078, 1330, 2150
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 4 cut(s) 467, 1530, 1788, 2084
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 179, 211
BpmI CTGGAG 2 cut(s) 1236, 1614
Bpu10I CCTNAGC 1 cut(s) 691
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 2028
BpuMI CCSGG 2 cut(s) 1754, 1755
Bsa29I ATCGAT 1 cut(s) 84
BsaAI YACGTR 2 cut(s) 1415, 1582
BsaI GGTCTC 1 cut(s) 1632
BsaJI CCNNGG 7 cut(s) 115, 163, 595, 944, 1465, 1753, 1754
BsaWI WCCGGW 1 cut(s) 1721
BsaXI ACNNNNNCTCC 2 cut(s) 1313, 1343
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 489, 1283, 1582
Bse118I RCCGGY 1 cut(s) 1586
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 873
BseAI TCCGGA 1 cut(s) 1721
BseBI CCWGG 2 cut(s) 116, 1467
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 7 cut(s) 115, 163, 595, 944, 1465, 1753, 1754
BseGI GGATG 5 cut(s) 328, 580, 1255, 1615, 2074
BseLI CCNNNNNNNGG 4 cut(s) 271, 489, 1283, 1582
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 5 cut(s) 347, 705, 1098, 1110, 1226
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 693, 2087
BsgI GTGCAG 1 cut(s) 1371
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 4 cut(s) 439, 594, 1586, 1974
BshNI GGYRCC 1 cut(s) 2038
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 2 cut(s) 1344, 1753
BsiSI CCGG 3 cut(s) 1587, 1722, 1754
BslFI GGGAC 3 cut(s) 365, 1027, 1072
BslI CCNNNNNNNGG 4 cut(s) 271, 489, 1283, 1582
BsmAI GTCTC 3 cut(s) 159, 307, 1632
BsmFI GGGAC 3 cut(s) 365, 1027, 1072
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 4 cut(s) 439, 594, 1586, 1974
Bso31I GGTCTC 1 cut(s) 1632
BsoBI CYCGRG 2 cut(s) 1344, 1753
Bsp1286I GDGCHC 2 cut(s) 550, 1188
Bsp13I TCCGGA 1 cut(s) 1721
Bsp1407I TGTACA 2 cut(s) 1127, 1320
Bsp143I GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 595, 944
BspACI CCGC 4 cut(s) 198, 265, 1577, 1698
BspANI GGCC 4 cut(s) 439, 594, 1586, 1974
BspCNI CTCAG 5 cut(s) 346, 704, 1099, 1111, 1227
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 1721
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 6 cut(s) 601, 687, 1561, 1720, 1867, 2040
BspMAI CTGCAG 2 cut(s) 622, 901
BspMI ACCTGC 1 cut(s) 1504
BspPI GGATC 5 cut(s) 591, 833, 1713, 1726, 1885
BspQI GCTCTTC 1 cut(s) 1293
BspT107I GGYRCC 1 cut(s) 2038
BspTNI GGTCTC 1 cut(s) 1632
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 1586
BsrGI TGTACA 2 cut(s) 1127, 1320
BsrI ACTGG 1 cut(s) 42
BssAI RCCGGY 1 cut(s) 1586
BssECI CCNNGG 7 cut(s) 115, 163, 595, 944, 1465, 1753, 1754
BssMI GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
BssT1I CCWWGG 3 cut(s) 163, 595, 944
Bst2UI CCWGG 2 cut(s) 116, 1467
Bst4CI ACNGT 8 cut(s) 834, 908, 1036, 1148, 1547, 1960, 2002, 2095
Bst6I CTCTTC 2 cut(s) 1293, 1414
BstAUI TGTACA 2 cut(s) 1127, 1320
BstBAI YACGTR 2 cut(s) 1415, 1582
BstC8I GCNNGC 5 cut(s) 282, 815, 819, 901, 1577
BstDEI CTNAG 8 cut(s) 6, 333, 691, 1107, 1119, 1141, 1235, 1776
BstDSI CCRYGG 2 cut(s) 595, 944
BstENI CCTNNNNNAGG 1 cut(s) 1281
BstF5I GGATG 5 cut(s) 328, 580, 1255, 1615, 2074
BstFNI CGCG 1 cut(s) 267
BstH2I RGCGCY 1 cut(s) 1464
BstHHI GCGC 1 cut(s) 1463
BstKTI GATC 8 cut(s) 347, 586, 841, 979, 1314, 1721, 1880, 1891
BstMAI GTCTC 3 cut(s) 159, 307, 1632
BstMBI GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
BstMWI GCNNNNNNNGC 8 cut(s) 617, 701, 854, 863, 938, 962, 1592, 1863
BstNI CCWGG 2 cut(s) 116, 1467
BstNSI RCATGY 2 cut(s) 1538, 2135
BstSCI CCNGG 4 cut(s) 114, 1465, 1752, 1753
BstSFI CTRYAG 8 cut(s) 618, 738, 897, 1032, 1659, 1746, 1860, 1998
BstUI CGCG 1 cut(s) 267
BstV2I GAAGAC 4 cut(s) 467, 1530, 1788, 2084
BstX2I RGATCY 3 cut(s) 344, 1718, 1877
BstYI RGATCY 3 cut(s) 344, 1718, 1877
Bsu15I ATCGAT 1 cut(s) 84
BsuI GTATCC 3 cut(s) 873, 942, 1372
BsuRI GGCC 4 cut(s) 439, 594, 1586, 1974
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 2 cut(s) 595, 944
BtsCI GGATG 5 cut(s) 328, 580, 1255, 1615, 2074
BtsI GCAGTG 1 cut(s) 1383
BtsIMutI CAGTG 6 cut(s) 258, 913, 1041, 1153, 1383, 1965
BveI ACCTGC 1 cut(s) 1504
Cac8I GCNNGC 5 cut(s) 282, 815, 819, 901, 1577
CfoI GCGC 1 cut(s) 1463
Cfr10I RCCGGY 1 cut(s) 1586
Cfr13I GGNCC 3 cut(s) 100, 911, 1418
Cfr9I CCCGGG 1 cut(s) 1753
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 9 cut(s) 112, 142, 669, 972, 1128, 1268, 1321, 1505, 1922
CviAII CATG 8 cut(s) 11, 70, 596, 863, 945, 1324, 1535, 2132
CviQI GTAC 9 cut(s) 112, 142, 669, 972, 1128, 1268, 1321, 1505, 1922
DdeI CTNAG 8 cut(s) 6, 333, 691, 1107, 1119, 1141, 1235, 1776
DpnI GATC 8 cut(s) 346, 585, 840, 978, 1313, 1720, 1879, 1890
DpnII GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
DraI TTTAAA 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 1584
Eam1104I CTCTTC 2 cut(s) 1293, 1414
EarI CTCTTC 2 cut(s) 1293, 1414
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 163, 595, 944
Eco24I GRGCYC 2 cut(s) 550, 1188
Eco31I GGTCTC 1 cut(s) 1632
Eco47I GGWCC 3 cut(s) 100, 911, 1418
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 2040
Eco72I CACGTG 2 cut(s) 1415, 1582
Eco88I CYCGRG 2 cut(s) 1344, 1753
EcoICRI GAGCTC 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 1281
EcoRI GAATTC 3 cut(s) 321, 1610, 2163
EcoRII CCWGG 2 cut(s) 114, 1465
EcoT14I CCWWGG 3 cut(s) 163, 595, 944
EcoT38I GRGCYC 2 cut(s) 550, 1188
ErhI CCWWGG 3 cut(s) 163, 595, 944
FaeI CATG 8 cut(s) 14, 73, 599, 866, 948, 1327, 1538, 2135
FaqI GGGAC 3 cut(s) 365, 1027, 1072
FatI CATG 8 cut(s) 10, 69, 595, 862, 944, 1323, 1534, 2131
FauI CCCGC 1 cut(s) 1584
FauNDI CATATG 2 cut(s) 1600, 1714
FbaI TGATCA 1 cut(s) 1311
FblI GTMKAC 1 cut(s) 169
FokI GGATG 5 cut(s) 315, 587, 1262, 1602, 2081
FriOI GRGCYC 2 cut(s) 550, 1188
FspBI CTAG 4 cut(s) 390, 659, 696, 1907
GlaI GCGC 1 cut(s) 1462
GsuI CTGGAG 2 cut(s) 1236, 1614
HaeII RGCGCY 1 cut(s) 1464
HaeIII GGCC 4 cut(s) 439, 594, 1586, 1974
HapII CCGG 3 cut(s) 1587, 1722, 1754
HhaI GCGC 1 cut(s) 1463
Hin1II CATG 8 cut(s) 14, 73, 599, 866, 948, 1327, 1538, 2135
Hin6I GCGC 1 cut(s) 1461
HinP1I GCGC 1 cut(s) 1461
HincII GTYRAC 2 cut(s) 787, 2026
HindII GTYRAC 2 cut(s) 787, 2026
HindIII AAGCTT 1 cut(s) 1770
HinfI GANTC 8 cut(s) 425, 432, 443, 920, 984, 1109, 1231, 1516
HpaII CCGG 3 cut(s) 1587, 1722, 1754
HphI GGTGA 5 cut(s) 138, 149, 1282, 1485, 1715
Hpy188I TCNGA 9 cut(s) 336, 448, 1108, 1193, 1236, 1311, 1870, 2116, 2158
HpyAV CCTTC 7 cut(s) 286, 500, 1127, 1198, 1414, 1911, 2065
HpyCH4III ACNGT 8 cut(s) 834, 908, 1036, 1148, 1547, 1960, 2002, 2095
HpyCH4IV ACGT 4 cut(s) 630, 996, 1414, 1581
HpyF10VI GCNNNNNNNGC 8 cut(s) 617, 701, 854, 863, 938, 962, 1592, 1863
HpyF3I CTNAG 8 cut(s) 6, 333, 691, 1107, 1119, 1141, 1235, 1776
HpySE526I ACGT 4 cut(s) 630, 996, 1414, 1581
Hsp92II CATG 8 cut(s) 14, 73, 599, 866, 948, 1327, 1538, 2135
HspAI GCGC 1 cut(s) 1461
Kpn2I TCCGGA 1 cut(s) 1721
Ksp22I TGATCA 1 cut(s) 1311
Kzo9I GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
LguI GCTCTTC 1 cut(s) 1293
LmnI GCTCC 5 cut(s) 14, 545, 565, 1217, 1871
LweI GCATC 7 cut(s) 565, 712, 757, 830, 1078, 1330, 2150
MaeI CTAG 4 cut(s) 390, 659, 696, 1907
MaeII ACGT 4 cut(s) 630, 996, 1414, 1581
MaeIII GTNAC 4 cut(s) 221, 1471, 1678, 2089
MalI GATC 8 cut(s) 346, 585, 840, 978, 1313, 1720, 1879, 1890
MboI GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
MboII GAAGA 8 cut(s) 58, 467, 1310, 1401, 1535, 1793, 1975, 2089
MfeI CAATTG 2 cut(s) 1263, 2184
MflI RGATCY 3 cut(s) 344, 1718, 1877
MhlI GDGCHC 2 cut(s) 550, 1188
MlyI GAGTC 2 cut(s) 1118, 1525
MmeI TCCRAC 1 cut(s) 1893
MroI TCCGGA 1 cut(s) 1721
MroXI GAANNNNTTC 2 cut(s) 65, 1043
MseI TTAA 9 cut(s) 456, 569, 1052, 1158, 1358, 1605, 1740, 1811, 1943
MslI CAYNNNNRTG 1 cut(s) 2033
MspA1I CMGCKG 2 cut(s) 200, 1700
MspI CCGG 3 cut(s) 1587, 1722, 1754
MspR9I CCNGG 4 cut(s) 116, 1467, 1754, 1755
MunI CAATTG 2 cut(s) 1263, 2184
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 2 cut(s) 116, 1467
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 8 cut(s) 617, 701, 854, 863, 938, 962, 1592, 1863
NciI CCSGG 2 cut(s) 1754, 1755
NcoI CCATGG 2 cut(s) 595, 944
NdeI CATATG 2 cut(s) 1600, 1714
NdeII GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
NlaIII CATG 8 cut(s) 14, 73, 599, 866, 948, 1327, 1538, 2135
NlaIV GGNNCC 6 cut(s) 601, 687, 1561, 1720, 1867, 2040
NmuCI GTSAC 1 cut(s) 2089
NspI RCATGY 2 cut(s) 1538, 2135
PaeR7I CTCGAG 1 cut(s) 1344
PaqCI CACCTGC 1 cut(s) 1504
PciI ACATGT 1 cut(s) 2131
PciSI GCTCTTC 1 cut(s) 1293
PcsI WCGNNNNNNNCGW 1 cut(s) 1002
PctI GAATGC 1 cut(s) 391
PdmI GAANNNNTTC 2 cut(s) 65, 1043
PfeI GAWTC 6 cut(s) 425, 432, 443, 920, 984, 1231
PflMI CCANNNNNTGG 1 cut(s) 489
PleI GAGTC 2 cut(s) 1117, 1524
PmaCI CACGTG 2 cut(s) 1415, 1582
PmlI CACGTG 2 cut(s) 1415, 1582
PpsI GAGTC 2 cut(s) 1117, 1524
Ppu21I YACGTR 2 cut(s) 1415, 1582
PscI ACATGT 1 cut(s) 2131
Psp124BI GAGCTC 1 cut(s) 550
Psp1406I AACGTT 2 cut(s) 630, 996
Psp6I CCWGG 2 cut(s) 114, 1465
PspCI CACGTG 2 cut(s) 1415, 1582
PspGI CCWGG 2 cut(s) 114, 1465
PspN4I GGNNCC 6 cut(s) 601, 687, 1561, 1720, 1867, 2040
PspPI GGNCC 3 cut(s) 100, 911, 1418
PspXI VCTCGAGB 1 cut(s) 1344
PstI CTGCAG 2 cut(s) 622, 901
PsuI RGATCY 3 cut(s) 344, 1718, 1877
RsaI GTAC 9 cut(s) 113, 143, 670, 973, 1129, 1269, 1322, 1506, 1923
RsaNI GTAC 9 cut(s) 112, 142, 669, 972, 1128, 1268, 1321, 1505, 1922
RseI CAYNNNNRTG 1 cut(s) 2033
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 1 cut(s) 1293
SaqAI TTAA 9 cut(s) 456, 569, 1052, 1158, 1358, 1605, 1740, 1811, 1943
Sau3AI GATC 8 cut(s) 344, 583, 838, 976, 1311, 1718, 1877, 1888
Sau96I GGNCC 3 cut(s) 100, 911, 1418
ScaI AGTACT 1 cut(s) 973
SchI GAGTC 2 cut(s) 1118, 1525
ScrFI CCNGG 4 cut(s) 116, 1467, 1754, 1755
SduI GDGCHC 2 cut(s) 550, 1188
SfaNI GCATC 7 cut(s) 565, 712, 757, 830, 1078, 1330, 2150
SfcI CTRYAG 8 cut(s) 618, 738, 897, 1032, 1659, 1746, 1860, 1998
Sfr274I CTCGAG 1 cut(s) 1344
SinI GGWCC 3 cut(s) 100, 911, 1418
SlaI CTCGAG 1 cut(s) 1344
SmaI CCCGGG 1 cut(s) 1755
SmiMI CAYNNNNRTG 1 cut(s) 2033
SmlI CTYRAG 2 cut(s) 1344, 2043
SmoI CTYRAG 2 cut(s) 1344, 2043
SsiI CCGC 4 cut(s) 198, 265, 1577, 1698
SspMI CTAG 4 cut(s) 390, 659, 696, 1907
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 4 cut(s) 114, 1465, 1752, 1753
StyI CCWWGG 3 cut(s) 163, 595, 944
TaaI ACNGT 8 cut(s) 834, 908, 1036, 1148, 1547, 1960, 2002, 2095
TaiI ACGT 4 cut(s) 633, 999, 1417, 1584
TaqI TCGA 5 cut(s) 84, 186, 1005, 1229, 1345
TaqII GACCGA 1 cut(s) 1835
TatI WGTACW 5 cut(s) 141, 971, 1127, 1320, 1504
TauI GCSGC 1 cut(s) 1700
TfiI GAWTC 6 cut(s) 425, 432, 443, 920, 984, 1231
Tru1I TTAA 9 cut(s) 456, 569, 1052, 1158, 1358, 1605, 1740, 1811, 1943
Tru9I TTAA 9 cut(s) 456, 569, 1052, 1158, 1358, 1605, 1740, 1811, 1943
TscAI CASTG 6 cut(s) 265, 913, 1041, 1153, 1390, 1965
TseFI GTSAC 1 cut(s) 2089
Tsp45I GTSAC 1 cut(s) 2089
TspGWI ACGGA 1 cut(s) 397
TspMI CCCGGG 1 cut(s) 1753
TspRI CASTG 6 cut(s) 265, 913, 1041, 1153, 1390, 1965
Van91I CCANNNNNTGG 1 cut(s) 489
VpaK11BI GGWCC 3 cut(s) 100, 911, 1418
XagI CCTNNNNNAGG 1 cut(s) 1281
XapI RAATTY 7 cut(s) 321, 517, 1423, 1610, 1706, 1945, 2163
XceI RCATGY 2 cut(s) 1538, 2135
XhoI CTCGAG 1 cut(s) 1344
XmaI CCCGGG 1 cut(s) 1753
XmiI GTMKAC 1 cut(s) 169
XmnI GAANNNNTTC 2 cut(s) 65, 1043
XspI CTAG 4 cut(s) 390, 659, 696, 1907
ZrmI AGTACT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.