MD07G1037500.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000257
Physical Location & Seq
Reverse (-)
3078265 .. 3079833
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1037500.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGGCAAAGCATGGATCTCTCCTGTATGCTTTCTCCATTATTCTCATGCTCGCTGTGAGCTTACCGTCTGAAGCCGCTGATCAGGAAGATAGAAAGACATATATTGTCTACTTGGGATCACTTCCTAAGGATCAGTTGTTCTCGCCATTGTCTCACCACATTGGTATTCTCGAAAGCATTACAGAGAGCAGCTCTCCGTCGAATCTCTTGGTCAGAAGCTACAAAAAGAGTTTCAACGGATTTGCTGCCAAGCTCACTGACCGAGAAAGAGAAAGGCTTGCTAACATGGAGGGAGTCGTCTCTGTGTTTCTGAGCACAACTTACCAACTCCAAACAACACGGTCTTGGGATTTCTTGGGTTTCAGTGAGAAGGTCAAACGAAACCCTACTGTCGAGAGCAATGTTATTGTTGGTGTCATCGACAGTGGGATTTGGCCTGAATCAAATAGCTTTAGTGATGAAGGTTTTGGGCCAGCTCCCAAAAAGTGGAAAGGAGTTTGCAAAGGTGGCTTCAATTTCACTTGCAACAACAAGCTCATTGGAGCACGGTATTACATTGATTCCACCGCTAGAGATGAAGTAGGACATGGAACTCATACTGCCTCAACAGCAGCAGGGAATCCCGTAGAGCCTGTGAGTTTTTATGGACTAGCTCAAGGCATTGCAAGAGGAGGAGTTCCCTCTGCGAGGATTGCTGCATATAAAGTTTGCGGTTCAGAAGGGTGCAGTGTAGATGGGATCTTGGCTGCTTTTGATGACGCCATTGCCGATGGAGTTGACATCATTACAATTTCAATTGCAAGTGAAACTGCTCCCCCGTTCCATGAGGATCCTATAGCAATCGGTGCTTTTCATGCAATGGAGAAAGGGATACTTACCTCGAATTCTGCAGGCAATTATGGCCCTGCAGAGCGTACTGTATCAAGTGTAGCACCATGGATTCTTTCAGTTGCAGCAAGTAGCACAGATCGTCGAATCATTGACAAGGTTGTTCTTGGAAATGGAAGGACACTCGTCGGTAGTTCAGTGAACACTTTGGAATTAAATGGAATGAGTTATCCGTTGATAGAAGGAAAGGATGCGTTAAGATCAAGTGGATGCGTGGACTGTGAAGATGGTTGCCTAGACCCTGATTTAGTTAAGGGAAACATTGTGGTATGTCGCCAGGCAAATGGCAGTATCGAGGCTCATCATGCCGGTGCAATAGGTGCAATTACAAGCACTACCCGATCTGATGTATCATTCGTTGTCCCACTACCTGCCACAAGTTTTAGTAGCAAAGATTATGATGTGGTGCATTCCTACCTCAACTCCACAGCTAAACCTGTAGGGAACATACTAAAAAGTGAAGTCATAAAAGACCTTGCTGCACCCACTGTCGCTTCCTTCTCTTCGCGGGGGCCTAATAATATTTTACCTGAAATTATCAAGCCAGACATAACTGCCCCGGGGGTTGAAATTTTGGCTGCATTTTCACCTGTTGCTTCTCTGACAGTTAGCTCTGAAGACCTAAAAAGGTCGTACCCAGTGCACAAGGCTCCCGCTATACGCAGGGTCTGGGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

523

Amino Acids

55.52

Weight (kDa)

5.93

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 111 8.9e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 133 - 491 1.7e-25 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1267
AccB7I CCANNNNNTGG 1 cut(s) 486
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 1396
AciI CCGC 5 cut(s) 75, 567, 711, 1396, 1542
AclWI GGATC 6 cut(s) 22, 124, 138, 746, 824, 837
AcsI RAATTY 2 cut(s) 883, 1458
AcuI CTGAAG 2 cut(s) 90, 1524
AcyI GRCGYC 1 cut(s) 759
AfaI GTAC 2 cut(s) 916, 1523
AfiI CCNNNNNNNGG 2 cut(s) 486, 687
AgsI TTSAA 4 cut(s) 235, 514, 795, 1457
AjnI CCWGG 1 cut(s) 1164
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
Alw21I GWGCWC 3 cut(s) 317, 547, 1533
Alw26I GTCTC 2 cut(s) 156, 304
Alw44I GTGCAC 1 cut(s) 1529
AlwI GGATC 6 cut(s) 22, 124, 138, 746, 824, 837
AlwNI CAGNNNCTG 1 cut(s) 1557
Ama87I CYCGRG 1 cut(s) 1447
AoxI GGCC 4 cut(s) 434, 470, 901, 1400
ApaLI GTGCAC 1 cut(s) 1529
ApeKI GCWGC 8 cut(s) 189, 245, 611, 695, 746, 953, 1367, 1466
ApoI RAATTY 2 cut(s) 883, 1458
AspS9I GGNCC 3 cut(s) 470, 902, 1400
AsuC2I CCSGG 2 cut(s) 1448, 1449
AsuHPI GGTGA 2 cut(s) 146, 1467
AvaI CYCGRG 1 cut(s) 1447
AxyI CCTNAGG 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 1533
BamHI GGATCC 1 cut(s) 829
BbsI GAAGAC 1 cut(s) 1512
Bbv12I GWGCWC 3 cut(s) 317, 547, 1533
BbvI GCAGC 8 cut(s) 201, 232, 623, 682, 733, 965, 1354, 1453
BccI CCATC 3 cut(s) 728, 764, 1109
BciT130I CCWGG 1 cut(s) 1166
BciVI GTATCC 1 cut(s) 864
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 2 cut(s) 1448, 1449
BcoDI GTCTC 2 cut(s) 156, 304
BfaI CTAG 3 cut(s) 570, 650, 1124
BfmI CTRYAG 4 cut(s) 834, 888, 906, 1326
BfuAI ACCTGC 1 cut(s) 1267
BfuI GTATCC 1 cut(s) 864
BisI GCNGC 9 cut(s) 75, 190, 246, 612, 696, 747, 954, 1368, 1467
BlsI GCNGC 9 cut(s) 76, 191, 247, 613, 697, 748, 955, 1369, 1468
Bme1390I CCNGG 3 cut(s) 1166, 1448, 1449
BmeT110I CYCGRG 1 cut(s) 1447
BmgT120I GGNCC 3 cut(s) 470, 902, 1400
BmiI GGNNCC 3 cut(s) 831, 1401, 1539
BmrFI CCNGG 3 cut(s) 1166, 1448, 1449
BmrI ACTGGG 1 cut(s) 1520
BmsI GCATC 2 cut(s) 1069, 1088
BmuI ACTGGG 1 cut(s) 1520
BoxI GACNNNNGTC 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 1512
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 178, 210
BpuEI CTTGAG 1 cut(s) 639
BpuMI CCSGG 2 cut(s) 1448, 1449
BsaHI GRCGYC 1 cut(s) 759
BsaJI CCNNGG 4 cut(s) 935, 1446, 1447, 1448
BsaXI ACNNNNNCTCC 2 cut(s) 1295, 1325
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 687
Bse118I RCCGGY 1 cut(s) 1196
Bse1I ACTGG 1 cut(s) 1526
Bse21I CCTNAGG 1 cut(s) 126
Bse3DI GCAATG 4 cut(s) 406, 660, 762, 864
BseBI CCWGG 1 cut(s) 1166
BseDI CCNNGG 4 cut(s) 935, 1446, 1447, 1448
BseGI GGATG 2 cut(s) 1084, 1103
BseLI CCNNNNNNNGG 2 cut(s) 486, 687
BseMI GCAATG 4 cut(s) 406, 660, 762, 864
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 1526
BseRI GAGGAG 2 cut(s) 684, 687
BseSI GKGCMC 1 cut(s) 1533
BseXI GCAGC 8 cut(s) 201, 232, 623, 682, 733, 965, 1354, 1453
BsgI GTGCAG 2 cut(s) 745, 1353
Bsh1236I CGCG 1 cut(s) 1396
BshFI GGCC 4 cut(s) 436, 472, 903, 1402
BsiHKAI GWGCWC 3 cut(s) 317, 547, 1533
BsiHKCI CYCGRG 1 cut(s) 1447
BsiSI CCGG 2 cut(s) 1197, 1448
BslFI GGGAC 1 cut(s) 1235
BslI CCNNNNNNNGG 2 cut(s) 486, 687
BsmAI GTCTC 2 cut(s) 156, 304
BsmBI CGTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 1235
BsmI GAATGC 1 cut(s) 1297
BsnI GGCC 4 cut(s) 436, 472, 903, 1402
BsoBI CYCGRG 1 cut(s) 1447
Bsp1286I GDGCHC 3 cut(s) 317, 547, 1533
Bsp143I GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
Bsp19I CCATGG 1 cut(s) 935
BspACI CCGC 5 cut(s) 75, 567, 711, 1396, 1542
BspANI GGCC 4 cut(s) 436, 472, 903, 1402
BspCNI CTCAG 1 cut(s) 303
BspFNI CGCG 1 cut(s) 1396
BspLI GGNNCC 3 cut(s) 831, 1401, 1539
BspMAI CTGCAG 2 cut(s) 892, 910
BspMI ACCTGC 1 cut(s) 1267
BspPI GGATC 6 cut(s) 22, 124, 138, 746, 824, 837
BsrDI GCAATG 4 cut(s) 406, 660, 762, 864
BsrFI RCCGGY 1 cut(s) 1196
BsrI ACTGG 1 cut(s) 1526
BssAI RCCGGY 1 cut(s) 1196
BssECI CCNNGG 4 cut(s) 935, 1446, 1447, 1448
BssMI GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
BssNI GRCGYC 1 cut(s) 759
BssT1I CCWWGG 1 cut(s) 935
Bst2UI CCWGG 1 cut(s) 1166
Bst4CI ACNGT 9 cut(s) 66, 342, 391, 425, 549, 919, 1109, 1378, 1495
Bst6I CTCTTC 1 cut(s) 1396
BstACI GRCGYC 1 cut(s) 759
BstAPI GCANNNNNTGC 2 cut(s) 845, 1208
BstC8I GCNNGC 4 cut(s) 51, 279, 474, 892
BstDEI CTNAG 2 cut(s) 126, 311
BstDSI CCRYGG 1 cut(s) 935
BstENI CCTNNNNNAGG 1 cut(s) 685
BstF5I GGATG 2 cut(s) 1084, 1103
BstFNI CGCG 1 cut(s) 1396
BstKTI GATC 9 cut(s) 17, 82, 119, 133, 741, 832, 970, 1091, 1232
BstMAI GTCTC 2 cut(s) 156, 304
BstMBI GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
BstMWI GCNNNNNNNGC 8 cut(s) 507, 608, 692, 845, 854, 900, 1193, 1208
BstNI CCWGG 1 cut(s) 1166
BstPAI GACNNNNGTC 1 cut(s) 1013
BstSCI CCNGG 3 cut(s) 1164, 1446, 1447
BstSFI CTRYAG 4 cut(s) 834, 888, 906, 1326
BstSLI GKGCMC 1 cut(s) 1533
BstUI CGCG 1 cut(s) 1396
BstV1I GCAGC 8 cut(s) 201, 232, 623, 682, 733, 965, 1354, 1453
BstV2I GAAGAC 1 cut(s) 1512
BstX2I RGATCY 3 cut(s) 14, 738, 829
BstXI CCANNNNNNTGG 1 cut(s) 1172
BstYI RGATCY 3 cut(s) 14, 738, 829
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 4 cut(s) 436, 472, 903, 1402
BtgI CCRYGG 1 cut(s) 935
BtsCI GGATG 2 cut(s) 1084, 1103
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 7 cut(s) 255, 370, 430, 733, 1032, 1374, 1533
BveI ACCTGC 1 cut(s) 1267
Cac8I GCNNGC 4 cut(s) 51, 279, 474, 892
CaiI CAGNNNCTG 1 cut(s) 1557
Cfr10I RCCGGY 1 cut(s) 1196
Cfr13I GGNCC 3 cut(s) 470, 902, 1400
Cfr9I CCCGGG 1 cut(s) 1447
CseI GACGC 1 cut(s) 767
Csp6I GTAC 2 cut(s) 915, 1522
CviAII CATG 8 cut(s) 11, 46, 286, 587, 824, 854, 936, 1193
CviQI GTAC 2 cut(s) 915, 1522
DdeI CTNAG 2 cut(s) 126, 311
DpnI GATC 9 cut(s) 16, 81, 118, 132, 740, 831, 969, 1090, 1231
DpnII GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
Eam1104I CTCTTC 1 cut(s) 1396
EarI CTCTTC 1 cut(s) 1396
Eco130I CCWWGG 1 cut(s) 935
Eco57I CTGAAG 2 cut(s) 90, 1524
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 1447
EcoNI CCTNNNNNAGG 1 cut(s) 685
EcoO109I RGGNCCY 1 cut(s) 1400
EcoRI GAATTC 1 cut(s) 883
EcoRII CCWGG 1 cut(s) 1164
EcoT14I CCWWGG 1 cut(s) 935
ErhI CCWWGG 1 cut(s) 935
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 8 cut(s) 14, 49, 289, 590, 827, 857, 939, 1196
FalI AAGNNNNNCTT 2 cut(s) 858, 890
FaqI GGGAC 1 cut(s) 1235
FatI CATG 8 cut(s) 10, 45, 285, 586, 823, 853, 935, 1192
FauI CCCGC 2 cut(s) 1389, 1549
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 9 cut(s) 75, 190, 246, 612, 696, 747, 954, 1368, 1467
FokI GGATG 2 cut(s) 1091, 1110
Fsp4HI GCNGC 9 cut(s) 75, 190, 246, 612, 696, 747, 954, 1368, 1467
FspBI CTAG 3 cut(s) 570, 650, 1124
GluI GCNGC 9 cut(s) 75, 190, 246, 612, 696, 747, 954, 1368, 1467
HaeIII GGCC 4 cut(s) 436, 472, 903, 1402
HapII CCGG 2 cut(s) 1197, 1448
HgaI GACGC 1 cut(s) 767
Hin1I GRCGYC 1 cut(s) 759
Hin1II CATG 8 cut(s) 14, 49, 289, 590, 827, 857, 939, 1196
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 7 cut(s) 202, 294, 440, 560, 619, 940, 975
HpaII CCGG 2 cut(s) 1197, 1448
HphI GGTGA 2 cut(s) 146, 1467
Hpy166II GTNNAC 5 cut(s) 109, 778, 1030, 1105, 1531
Hpy188I TCNGA 7 cut(s) 70, 215, 312, 718, 1234, 1491, 1504
Hpy188III TCNNGA 3 cut(s) 83, 170, 394
Hpy8I GTNNAC 5 cut(s) 109, 778, 1030, 1105, 1531
Hpy99I CGWCG 3 cut(s) 202, 975, 1019
HpyAV CCTTC 6 cut(s) 364, 455, 713, 999, 1064, 1396
HpyCH4III ACNGT 9 cut(s) 66, 342, 391, 425, 549, 919, 1109, 1378, 1495
HpyF10VI GCNNNNNNNGC 8 cut(s) 507, 608, 692, 845, 854, 900, 1193, 1208
HpyF3I CTNAG 2 cut(s) 126, 311
Hsp92I GRCGYC 1 cut(s) 759
Hsp92II CATG 8 cut(s) 14, 49, 289, 590, 827, 857, 939, 1196
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
LmnI GCTCC 4 cut(s) 481, 542, 817, 1543
Lsp1109I GCAGC 8 cut(s) 201, 232, 623, 682, 733, 965, 1354, 1453
LweI GCATC 2 cut(s) 1069, 1088
MaeI CTAG 3 cut(s) 570, 650, 1124
MalI GATC 9 cut(s) 16, 81, 118, 132, 740, 831, 969, 1090, 1231
MboI GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
MboII GAAGA 4 cut(s) 98, 1124, 1383, 1517
MfeI CAATTG 1 cut(s) 795
MflI RGATCY 3 cut(s) 14, 738, 829
MhlI GDGCHC 3 cut(s) 317, 547, 1533
MluCI AATT 9 cut(s) 514, 789, 795, 883, 895, 1040, 1212, 1422, 1458
MlyI GAGTC 1 cut(s) 303
MseI TTAA 3 cut(s) 1043, 1085, 1140
MslI CAYNNNNRTG 1 cut(s) 1197
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 2 cut(s) 1197, 1448
MspR9I CCNGG 3 cut(s) 1166, 1448, 1449
MunI CAATTG 1 cut(s) 795
Mva1269I GAATGC 1 cut(s) 1297
MvaI CCWGG 1 cut(s) 1166
MvnI CGCG 1 cut(s) 1396
MwoI GCNNNNNNNGC 8 cut(s) 507, 608, 692, 845, 854, 900, 1193, 1208
NciI CCSGG 2 cut(s) 1448, 1449
NcoI CCATGG 1 cut(s) 935
NdeII GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
NlaIII CATG 8 cut(s) 14, 49, 289, 590, 827, 857, 939, 1196
NlaIV GGNNCC 3 cut(s) 831, 1401, 1539
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PctI GAATGC 1 cut(s) 1297
PfeI GAWTC 6 cut(s) 202, 440, 560, 619, 940, 975
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 9 cut(s) 76, 191, 247, 613, 697, 748, 955, 1369, 1468
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PshAI GACNNNNGTC 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 1164
PspGI CCWGG 1 cut(s) 1164
PspN4I GGNNCC 3 cut(s) 831, 1401, 1539
PspPI GGNCC 3 cut(s) 470, 902, 1400
PstI CTGCAG 2 cut(s) 892, 910
PstNI CAGNNNCTG 1 cut(s) 1557
PsuI RGATCY 3 cut(s) 14, 738, 829
RsaI GTAC 2 cut(s) 916, 1523
RsaNI GTAC 2 cut(s) 915, 1522
RseI CAYNNNNRTG 1 cut(s) 1197
SaqAI TTAA 3 cut(s) 1043, 1085, 1140
SatI GCNGC 9 cut(s) 75, 190, 246, 612, 696, 747, 954, 1368, 1467
Sau3AI GATC 9 cut(s) 14, 79, 116, 130, 738, 829, 967, 1088, 1229
Sau96I GGNCC 3 cut(s) 470, 902, 1400
SchI GAGTC 1 cut(s) 303
ScrFI CCNGG 3 cut(s) 1166, 1448, 1449
SduI GDGCHC 3 cut(s) 317, 547, 1533
SfaNI GCATC 2 cut(s) 1069, 1088
SfcI CTRYAG 4 cut(s) 834, 888, 906, 1326
SmaI CCCGGG 1 cut(s) 1449
SmiMI CAYNNNNRTG 1 cut(s) 1197
SmlI CTYRAG 1 cut(s) 654
SmoI CTYRAG 1 cut(s) 654
Sse9I AATT 9 cut(s) 514, 789, 795, 883, 895, 1040, 1212, 1422, 1458
SsiI CCGC 5 cut(s) 75, 567, 711, 1396, 1542
SspI AATATT 1 cut(s) 1411
SspMI CTAG 3 cut(s) 570, 650, 1124
StyD4I CCNGG 3 cut(s) 1164, 1446, 1447
StyI CCWWGG 1 cut(s) 935
TaaI ACNGT 9 cut(s) 66, 342, 391, 425, 549, 919, 1109, 1378, 1495
TaqI TCGA 7 cut(s) 171, 200, 393, 420, 881, 973, 1182
TaqII GACCGA 1 cut(s) 276
TasI AATT 9 cut(s) 514, 789, 795, 883, 895, 1040, 1212, 1422, 1458
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 6 cut(s) 202, 440, 560, 619, 940, 975
Tru1I TTAA 3 cut(s) 1043, 1085, 1140
Tru9I TTAA 3 cut(s) 1043, 1085, 1140
TscAI CASTG 7 cut(s) 262, 370, 430, 733, 1032, 1381, 1533
TseI GCWGC 8 cut(s) 189, 245, 611, 695, 746, 953, 1367, 1466
TspDTI ATGAA 3 cut(s) 474, 591, 842
TspGWI ACGGA 3 cut(s) 186, 252, 1050
TspMI CCCGGG 1 cut(s) 1447
TspRI CASTG 7 cut(s) 262, 370, 430, 733, 1032, 1381, 1533
Van91I CCANNNNNTGG 1 cut(s) 486
VneI GTGCAC 1 cut(s) 1529
XagI CCTNNNNNAGG 1 cut(s) 685
XapI RAATTY 2 cut(s) 883, 1458
XmaI CCCGGG 1 cut(s) 1447
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 3 cut(s) 570, 650, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.