cucumisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 3143 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 476, 2906 |
| Acc36I | ACCTGC | 1 cut(s) | 1315 |
| AccB1I | GGYRCC | 2 cut(s) | 1592, 1608 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 573, 1605, 2631 |
| AccI | GTMKAC | 2 cut(s) | 1104, 2985 |
| AciI | CCGC | 5 cut(s) | 162, 1617, 1652, 2682, 3364 |
| AclWI | GGATC | 9 cut(s) | 211, 1019, 1403, 1847, 1857, 2269, 2381, 2717, 3198 |
| AcsI | RAATTY | 9 cut(s) | 12, 601, 1736, 1907, 2116, 2347, 2494, 2528, 3500 |
| AcuI | CTGAAG | 1 cut(s) | 3591 |
| AcyI | GRCGYC | 2 cut(s) | 153, 1609 |
| AfaI | GTAC | 7 cut(s) | 42, 197, 844, 952, 1779, 3233, 3560 |
| AhlI | ACTAGT | 1 cut(s) | 2791 |
| AjiI | CACGTC | 1 cut(s) | 670 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1414, 1446 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1078, 1110 |
| Alw21I | GWGCWC | 3 cut(s) | 94, 2158, 3594 |
| Alw26I | GTCTC | 9 cut(s) | 243, 391, 462, 1665, 1686, 1793, 2301, 2449, 3104 |
| AlwI | GGATC | 9 cut(s) | 211, 1019, 1403, 1847, 1857, 2269, 2381, 2717, 3198 |
| Ama87I | CYCGRG | 3 cut(s) | 1374, 3255, 3298 |
| AoxI | GGCC | 9 cut(s) | 521, 1700, 1933, 1956, 2504, 2579, 3280, 3550, 3612 |
| ApaI | GGGCCC | 1 cut(s) | 2508 |
| ApoI | RAATTY | 9 cut(s) | 12, 601, 1736, 1907, 2116, 2347, 2494, 2528, 3500 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 536, 568 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 14, 2039, 2530, 2951 |
| AspLEI | GCGC | 3 cut(s) | 970, 1403, 1611 |
| AsuC2I | CCSGG | 5 cut(s) | 1553, 1785, 3299, 3300, 3549 |
| AsuHPI | GGTGA | 9 cut(s) | 86, 233, 1406, 1515, 1745, 2097, 2291, 2956, 3509 |
| AsuII | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| AsuNHI | GCTAGC | 1 cut(s) | 1867 |
| AvaI | CYCGRG | 3 cut(s) | 1374, 3255, 3298 |
| AvaII | GGWCC | 6 cut(s) | 557, 938, 1347, 1448, 2615, 2819 |
| BaeGI | GKGCMC | 3 cut(s) | 1495, 2508, 3298 |
| BanI | GGYRCC | 2 cut(s) | 1592, 1608 |
| BanII | GRGCYC | 3 cut(s) | 94, 2158, 2508 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 268, 300 |
| BbsI | GAAGAC | 1 cut(s) | 1563 |
| Bbv12I | GWGCWC | 3 cut(s) | 94, 2158, 3594 |
| BbvCI | CCTCAGC | 1 cut(s) | 2653 |
| BccI | CCATC | 8 cut(s) | 800, 1031, 1594, 1679, 2051, 2684, 2735, 3479 |
| BceAI | ACGGC | 2 cut(s) | 930, 2204 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 782, 816 |
| BciVI | GTATCC | 4 cut(s) | 900, 969, 2781, 2850 |
| BclI | TGATCA | 4 cut(s) | 166, 1207, 2224, 3088 |
| BcnI | CCSGG | 5 cut(s) | 1553, 1785, 3299, 3300, 3549 |
| BcoDI | GTCTC | 9 cut(s) | 243, 391, 462, 1665, 1686, 1793, 2301, 2449, 3104 |
| BcuI | ACTAGT | 1 cut(s) | 2791 |
| BfaI | CTAG | 7 cut(s) | 147, 1157, 1868, 2199, 2792, 3050, 3652 |
| BfmI | CTRYAG | 3 cut(s) | 924, 2805, 3462 |
| BfoI | RGCGCY | 2 cut(s) | 971, 1612 |
| BfuAI | ACCTGC | 1 cut(s) | 1315 |
| BfuI | GTATCC | 4 cut(s) | 900, 969, 2781, 2850 |
| BglII | AGATCT | 1 cut(s) | 2486 |
| BlpI | GCTNAGC | 1 cut(s) | 142 |
| Bme18I | GGWCC | 6 cut(s) | 557, 938, 1347, 1448, 2615, 2819 |
| BmeT110I | CYCGRG | 3 cut(s) | 1374, 3255, 3298 |
| BmgBI | CACGTC | 1 cut(s) | 670 |
| BmiI | GGNNCC | 8 cut(s) | 714, 940, 1449, 1533, 1594, 1610, 2506, 3394 |
| BmtI | GCTAGC | 1 cut(s) | 1871 |
| BpiI | GAAGAC | 1 cut(s) | 1563 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 212, 244, 2321, 2353 |
| BpmI | CTGGAG | 3 cut(s) | 1647, 3375, 3477 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2653 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 142 |
| Bpu14I | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| BpuMI | CCSGG | 5 cut(s) | 1553, 1785, 3299, 3300, 3549 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3202 |
| BsaHI | GRCGYC | 2 cut(s) | 153, 1609 |
| BsaI | GGTCTC | 3 cut(s) | 462, 1665, 1793 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1343, 1373, 3224, 3254 |
| Bse118I | RCCGGY | 1 cut(s) | 1619 |
| Bse1I | ACTGG | 3 cut(s) | 1013, 1661, 3329 |
| Bse3DI | GCAATG | 2 cut(s) | 3154, 3182 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3202 |
| BseGI | GGATG | 8 cut(s) | 667, 816, 871, 1169, 1273, 1780, 3050, 3613 |
| BseJI | GATNNNNATC | 1 cut(s) | 3202 |
| BseMI | GCAATG | 2 cut(s) | 3154, 3182 |
| BseMII | CTCAG | 4 cut(s) | 1265, 1557, 2667, 3146 |
| BseNI | ACTGG | 3 cut(s) | 1013, 1661, 3329 |
| BseRI | GAGGAG | 5 cut(s) | 233, 328, 720, 1429, 2045 |
| BseSI | GKGCMC | 3 cut(s) | 1495, 2508, 3298 |
| BseYI | CCCAGC | 2 cut(s) | 1844, 1969 |
| BsgI | GTGCAG | 4 cut(s) | 1401, 1644, 3147, 3444 |
| BshFI | GGCC | 9 cut(s) | 523, 1702, 1935, 1958, 2506, 2581, 3282, 3552, 3614 |
| BshNI | GGYRCC | 2 cut(s) | 1592, 1608 |
| BsiHKAI | GWGCWC | 3 cut(s) | 94, 2158, 3594 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1374, 3255, 3298 |
| BsiSI | CCGG | 5 cut(s) | 1553, 1620, 1784, 3299, 3548 |
| BslFI | GGGAC | 4 cut(s) | 449, 924, 1461, 3164 |
| BsmAI | GTCTC | 9 cut(s) | 243, 391, 462, 1665, 1686, 1793, 2301, 2449, 3104 |
| BsmBI | CGTCTC | 2 cut(s) | 391, 1686 |
| BsmFI | GGGAC | 4 cut(s) | 449, 924, 1461, 3164 |
| BsmI | GAATGC | 2 cut(s) | 2646, 3443 |
| BsnI | GGCC | 9 cut(s) | 523, 1702, 1935, 1958, 2506, 2581, 3282, 3552, 3614 |
| Bso31I | GGTCTC | 3 cut(s) | 462, 1665, 1793 |
| BsoBI | CYCGRG | 3 cut(s) | 1374, 3255, 3298 |
| Bsp119I | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| Bsp120I | GGGCCC | 1 cut(s) | 2504 |
| Bsp1286I | GDGCHC | 6 cut(s) | 94, 1495, 2158, 2508, 3298, 3594 |
| Bsp1407I | TGTACA | 1 cut(s) | 1777 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 142 |
| BspACI | CCGC | 5 cut(s) | 162, 1617, 1652, 2682, 3364 |
| BspANI | GGCC | 9 cut(s) | 523, 1702, 1935, 1958, 2506, 2581, 3282, 3552, 3614 |
| BspCNI | CTCAG | 4 cut(s) | 1266, 1556, 2666, 3147 |
| BspLI | GGNNCC | 8 cut(s) | 714, 940, 1449, 1533, 1594, 1610, 2506, 3394 |
| BspMAI | CTGCAG | 3 cut(s) | 928, 2809, 3466 |
| BspMI | ACCTGC | 1 cut(s) | 1315 |
| BspOI | GCTAGC | 1 cut(s) | 1871 |
| BspPI | GGATC | 9 cut(s) | 211, 1019, 1403, 1847, 1857, 2269, 2381, 2717, 3198 |
| BspT104I | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| BspT107I | GGYRCC | 2 cut(s) | 1592, 1608 |
| BspTNI | GGTCTC | 3 cut(s) | 462, 1665, 1793 |
| BsrDI | GCAATG | 2 cut(s) | 3154, 3182 |
| BsrFI | RCCGGY | 1 cut(s) | 1619 |
| BsrGI | TGTACA | 1 cut(s) | 1777 |
| BsrI | ACTGG | 3 cut(s) | 1013, 1661, 3329 |
| BssAI | RCCGGY | 1 cut(s) | 1619 |
| BssNAI | GTATAC | 2 cut(s) | 1105, 2986 |
| BssNI | GRCGYC | 2 cut(s) | 153, 1609 |
| BssT1I | CCWWGG | 6 cut(s) | 80, 1020, 2815, 2852, 2911, 3603 |
| Bst1107I | GTATAC | 2 cut(s) | 1105, 2986 |
| Bst6I | CTCTTC | 1 cut(s) | 213 |
| BstACI | GRCGYC | 2 cut(s) | 153, 1609 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 728, 923, 2804 |
| BstAUI | TGTACA | 1 cut(s) | 1777 |
| BstBI | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| BstC8I | GCNNGC | 7 cut(s) | 928, 1143, 1869, 2172, 2424, 2809, 3189 |
| BstDEI | CTNAG | 5 cut(s) | 142, 1274, 1543, 2653, 3155 |
| BstDSI | CCRYGG | 1 cut(s) | 1527 |
| BstF5I | GGATG | 8 cut(s) | 667, 816, 871, 1169, 1273, 1780, 3050, 3613 |
| BstH2I | RGCGCY | 2 cut(s) | 971, 1612 |
| BstHHI | GCGC | 3 cut(s) | 970, 1403, 1611 |
| BstMAI | GTCTC | 9 cut(s) | 243, 391, 462, 1665, 1686, 1793, 2301, 2449, 3104 |
| BstNSI | RCATGY | 3 cut(s) | 1236, 2174, 2214 |
| BstSFI | CTRYAG | 3 cut(s) | 924, 2805, 3462 |
| BstSLI | GKGCMC | 3 cut(s) | 1495, 2508, 3298 |
| BstV2I | GAAGAC | 1 cut(s) | 1563 |
| BstX2I | RGATCY | 7 cut(s) | 203, 1408, 1849, 2373, 2486, 2709, 3203 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3419 |
| BstYI | RGATCY | 7 cut(s) | 203, 1408, 1849, 2373, 2486, 2709, 3203 |
| BstZ17I | GTATAC | 2 cut(s) | 1105, 2986 |
| BsuI | GTATCC | 4 cut(s) | 900, 969, 2781, 2850 |
| BsuRI | GGCC | 9 cut(s) | 523, 1702, 1935, 1958, 2506, 2581, 3282, 3552, 3614 |
| BtgI | CCRYGG | 1 cut(s) | 1527 |
| BtrI | CACGTC | 1 cut(s) | 670 |
| BtsCI | GGATG | 8 cut(s) | 667, 816, 871, 1169, 1273, 1780, 3050, 3613 |
| BtsI | GCAGTG | 1 cut(s) | 900 |
| BtsIMutI | CAGTG | 9 cut(s) | 517, 900, 1006, 1654, 2043, 2096, 2400, 2575, 3061 |
| BveI | ACCTGC | 1 cut(s) | 1315 |
| Cac8I | GCNNGC | 7 cut(s) | 928, 1143, 1869, 2172, 2424, 2809, 3189 |
| CfoI | GCGC | 3 cut(s) | 970, 1403, 1611 |
| Cfr10I | RCCGGY | 1 cut(s) | 1619 |
| Cfr9I | CCCGGG | 1 cut(s) | 3298 |
| CseI | GACGC | 3 cut(s) | 161, 1379, 2988 |
| CsiI | ACCWGGT | 1 cut(s) | 96 |
| Csp6I | GTAC | 7 cut(s) | 41, 196, 843, 951, 1778, 3232, 3559 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 37, 72, 3437, 3472 |
| CviQI | GTAC | 7 cut(s) | 41, 196, 843, 951, 1778, 3232, 3559 |
| DdeI | CTNAG | 5 cut(s) | 142, 1274, 1543, 2653, 3155 |
| DinI | GGCGCC | 1 cut(s) | 1610 |
| DraI | TTTAAA | 3 cut(s) | 541, 1770, 2599 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 476, 2906 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 476, 2906 |
| Eam1104I | CTCTTC | 1 cut(s) | 213 |
| EarI | CTCTTC | 1 cut(s) | 213 |
| Ecl136II | GAGCTC | 2 cut(s) | 92, 2156 |
| Eco130I | CCWWGG | 6 cut(s) | 80, 1020, 2815, 2852, 2911, 3603 |
| Eco24I | GRGCYC | 3 cut(s) | 94, 2158, 2508 |
| Eco31I | GGTCTC | 3 cut(s) | 462, 1665, 1793 |
| Eco47I | GGWCC | 6 cut(s) | 557, 938, 1347, 1448, 2615, 2819 |
| Eco53kI | GAGCTC | 2 cut(s) | 92, 2156 |
| Eco57I | CTGAAG | 1 cut(s) | 3591 |
| Eco88I | CYCGRG | 3 cut(s) | 1374, 3255, 3298 |
| EcoICRI | GAGCTC | 2 cut(s) | 92, 2156 |
| EcoO109I | RGGNCCY | 3 cut(s) | 938, 1347, 2819 |
| EcoT14I | CCWWGG | 6 cut(s) | 80, 1020, 2815, 2852, 2911, 3603 |
| EcoT22I | ATGCAT | 3 cut(s) | 1120, 1710, 2080 |
| EcoT38I | GRGCYC | 3 cut(s) | 94, 2158, 2508 |
| EgeI | GGCGCC | 1 cut(s) | 1610 |
| EheI | GGCGCC | 1 cut(s) | 1610 |
| ErhI | CCWWGG | 6 cut(s) | 80, 1020, 2815, 2852, 2911, 3603 |
| Esp3I | CGTCTC | 2 cut(s) | 391, 1686 |
| FaqI | GGGAC | 4 cut(s) | 449, 924, 1461, 3164 |
| FauI | CCCGC | 1 cut(s) | 1659 |
| FauNDI | CATATG | 1 cut(s) | 2083 |
| FbaI | TGATCA | 4 cut(s) | 166, 1207, 2224, 3088 |
| FblI | GTMKAC | 2 cut(s) | 1104, 2985 |
| FokI | GGATG | 8 cut(s) | 674, 803, 878, 1176, 1280, 1767, 3057, 3620 |
| FriOI | GRGCYC | 3 cut(s) | 94, 2158, 2508 |
| FspBI | CTAG | 7 cut(s) | 147, 1157, 1868, 2199, 2792, 3050, 3652 |
| GlaI | GCGC | 3 cut(s) | 969, 1402, 1610 |
| GsaI | CCCAGC | 2 cut(s) | 1848, 1973 |
| GsuI | CTGGAG | 3 cut(s) | 1647, 3375, 3477 |
| HaeII | RGCGCY | 2 cut(s) | 971, 1612 |
| HaeIII | GGCC | 9 cut(s) | 523, 1702, 1935, 1958, 2506, 2581, 3282, 3552, 3614 |
| HapII | CCGG | 5 cut(s) | 1553, 1620, 1784, 3299, 3548 |
| HgaI | GACGC | 3 cut(s) | 161, 1379, 2988 |
| HhaI | GCGC | 3 cut(s) | 970, 1403, 1611 |
| Hin1I | GRCGYC | 2 cut(s) | 153, 1609 |
| Hin6I | GCGC | 3 cut(s) | 968, 1401, 1609 |
| HinP1I | GCGC | 3 cut(s) | 968, 1401, 1609 |
| HincII | GTYRAC | 2 cut(s) | 814, 2749 |
| HindII | GTYRAC | 2 cut(s) | 814, 2749 |
| HindIII | AAGCTT | 1 cut(s) | 2471 |
| HpaII | CCGG | 5 cut(s) | 1553, 1620, 1784, 3299, 3548 |
| HphI | GGTGA | 9 cut(s) | 86, 233, 1406, 1515, 1745, 2097, 2291, 2956, 3509 |
| Hpy166II | GTNNAC | 8 cut(s) | 196, 814, 1105, 2112, 2749, 2947, 2986, 3559 |
| Hpy188I | TCNGA | 9 cut(s) | 399, 532, 769, 881, 1275, 1546, 2689, 3156, 3571 |
| Hpy8I | GTNNAC | 8 cut(s) | 196, 814, 1105, 2112, 2749, 2947, 2986, 3559 |
| Hpy99I | CGWCG | 2 cut(s) | 758, 1373 |
| HpyCH4IV | ACGT | 2 cut(s) | 669, 3234 |
| HpyF3I | CTNAG | 5 cut(s) | 142, 1274, 1543, 2653, 3155 |
| HpySE526I | ACGT | 2 cut(s) | 669, 3234 |
| Hsp92I | GRCGYC | 2 cut(s) | 153, 1609 |
| HspAI | GCGC | 3 cut(s) | 968, 1401, 1609 |
| KasI | GGCGCC | 1 cut(s) | 1608 |
| Ksp22I | TGATCA | 4 cut(s) | 166, 1207, 2224, 3088 |
| LmnI | GCTCC | 9 cut(s) | 89, 315, 568, 629, 1537, 1833, 2153, 2626, 3245 |
| MabI | ACCWGGT | 1 cut(s) | 96 |
| MaeI | CTAG | 7 cut(s) | 147, 1157, 1868, 2199, 2792, 3050, 3652 |
| MaeII | ACGT | 2 cut(s) | 669, 3234 |
| MaeIII | GTNAC | 6 cut(s) | 149, 995, 1879, 2047, 2103, 3340 |
| MfeI | CAATTG | 2 cut(s) | 984, 1422 |
| MflI | RGATCY | 7 cut(s) | 203, 1408, 1849, 2373, 2486, 2709, 3203 |
| MhlI | GDGCHC | 6 cut(s) | 94, 1495, 2158, 2508, 3298, 3594 |
| Mly113I | GGCGCC | 1 cut(s) | 1609 |
| MlyI | GAGTC | 1 cut(s) | 3484 |
| MmeI | TCCRAC | 3 cut(s) | 814, 1855, 2749 |
| Mph1103I | ATGCAT | 3 cut(s) | 1120, 1710, 2080 |
| MroXI | GAANNNNTTC | 4 cut(s) | 14, 2039, 2530, 2951 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1598, 3587 |
| MspA1I | CMGCKG | 3 cut(s) | 164, 1628, 3428 |
| MspI | CCGG | 5 cut(s) | 1553, 1620, 1784, 3299, 3548 |
| MunI | CAATTG | 2 cut(s) | 984, 1422 |
| Mva1269I | GAATGC | 2 cut(s) | 2646, 3443 |
| NarI | GGCGCC | 1 cut(s) | 1609 |
| NciI | CCSGG | 5 cut(s) | 1553, 1785, 3299, 3300, 3549 |
| NdeI | CATATG | 1 cut(s) | 2083 |
| NheI | GCTAGC | 1 cut(s) | 1867 |
| NlaIV | GGNNCC | 8 cut(s) | 714, 940, 1449, 1533, 1594, 1610, 2506, 3394 |
| NmeAIII | GCCGAG | 2 cut(s) | 111, 2186 |
| NmuCI | GTSAC | 3 cut(s) | 149, 2047, 2103 |
| NsiI | ATGCAT | 3 cut(s) | 1120, 1710, 2080 |
| NspI | RCATGY | 3 cut(s) | 1236, 2174, 2214 |
| NspV | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| PaeI | GCATGC | 1 cut(s) | 2174 |
| PaeR7I | CTCGAG | 2 cut(s) | 1374, 3255 |
| PasI | CCCWGGG | 1 cut(s) | 2827 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1145 |
| PctI | GAATGC | 2 cut(s) | 2646, 3443 |
| PdmI | GAANNNNTTC | 4 cut(s) | 14, 2039, 2530, 2951 |
| PfeI | GAWTC | 9 cut(s) | 289, 327, 381, 516, 527, 1136, 2385, 2574, 3017 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 573, 1605, 2631 |
| PleI | GAGTC | 1 cut(s) | 3484 |
| PluTI | GGCGCC | 1 cut(s) | 1612 |
| PpsI | GAGTC | 1 cut(s) | 3484 |
| PpuMI | RGGWCCY | 3 cut(s) | 938, 1347, 2819 |
| PsiI | TTATAA | 1 cut(s) | 3143 |
| Psp124BI | GAGCTC | 2 cut(s) | 94, 2158 |
| Psp5II | RGGWCCY | 3 cut(s) | 938, 1347, 2819 |
| PspFI | CCCAGC | 2 cut(s) | 1844, 1969 |
| PspN4I | GGNNCC | 8 cut(s) | 714, 940, 1449, 1533, 1594, 1610, 2506, 3394 |
| PspOMI | GGGCCC | 1 cut(s) | 2504 |
| PspPPI | RGGWCCY | 3 cut(s) | 938, 1347, 2819 |
| PspXI | VCTCGAGB | 2 cut(s) | 1374, 3255 |
| PstI | CTGCAG | 3 cut(s) | 928, 2809, 3466 |
| PsuI | RGATCY | 7 cut(s) | 203, 1408, 1849, 2373, 2486, 2709, 3203 |
| PvuII | CAGCTG | 2 cut(s) | 1628, 3428 |
| RsaI | GTAC | 7 cut(s) | 42, 197, 844, 952, 1779, 3233, 3560 |
| RsaNI | GTAC | 7 cut(s) | 41, 196, 843, 951, 1778, 3232, 3559 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1598, 3587 |
| SacI | GAGCTC | 2 cut(s) | 94, 2158 |
| SchI | GAGTC | 1 cut(s) | 3484 |
| SduI | GDGCHC | 6 cut(s) | 94, 1495, 2158, 2508, 3298, 3594 |
| SexAI | ACCWGGT | 1 cut(s) | 96 |
| SfcI | CTRYAG | 3 cut(s) | 924, 2805, 3462 |
| SfoI | GGCGCC | 1 cut(s) | 1610 |
| Sfr274I | CTCGAG | 2 cut(s) | 1374, 3255 |
| SfuI | TTCGAA | 3 cut(s) | 287, 1639, 3628 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1619 |
| SinI | GGWCC | 6 cut(s) | 557, 938, 1347, 1448, 2615, 2819 |
| SlaI | CTCGAG | 2 cut(s) | 1374, 3255 |
| SmaI | CCCGGG | 1 cut(s) | 3300 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1598, 3587 |
| SmlI | CTYRAG | 2 cut(s) | 1374, 3255 |
| SmoI | CTYRAG | 2 cut(s) | 1374, 3255 |
| SpeI | ACTAGT | 1 cut(s) | 2791 |
| SphI | GCATGC | 1 cut(s) | 2174 |
| SsiI | CCGC | 5 cut(s) | 162, 1617, 1652, 2682, 3364 |
| SspDI | GGCGCC | 1 cut(s) | 1608 |
| SspMI | CTAG | 7 cut(s) | 147, 1157, 1868, 2199, 2792, 3050, 3652 |
| SstI | GAGCTC | 2 cut(s) | 94, 2158 |
| StyI | CCWWGG | 6 cut(s) | 80, 1020, 2815, 2852, 2911, 3603 |
| TaiI | ACGT | 2 cut(s) | 672, 3237 |
| TatI | WGTACW | 2 cut(s) | 195, 1777 |
| TauI | GCSGC | 3 cut(s) | 164, 1619, 3367 |
| TfiI | GAWTC | 9 cut(s) | 289, 327, 381, 516, 527, 1136, 2385, 2574, 3017 |
| TscAI | CASTG | 9 cut(s) | 517, 900, 1013, 1661, 2050, 2103, 2407, 2575, 3061 |
| TseFI | GTSAC | 3 cut(s) | 149, 2047, 2103 |
| Tsp45I | GTSAC | 3 cut(s) | 149, 2047, 2103 |
| TspGWI | ACGGA | 7 cut(s) | 102, 1098, 1122, 1991, 2166, 2603, 3003 |
| TspMI | CCCGGG | 1 cut(s) | 3298 |
| TspRI | CASTG | 9 cut(s) | 517, 900, 1013, 1661, 2050, 2103, 2407, 2575, 3061 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 573, 1605, 2631 |
| VpaK11BI | GGWCC | 6 cut(s) | 557, 938, 1347, 1448, 2615, 2819 |
| XapI | RAATTY | 9 cut(s) | 12, 601, 1736, 1907, 2116, 2347, 2494, 2528, 3500 |
| XbaI | TCTAGA | 1 cut(s) | 1156 |
| XceI | RCATGY | 3 cut(s) | 1236, 2174, 2214 |
| XhoI | CTCGAG | 2 cut(s) | 1374, 3255 |
| XmaI | CCCGGG | 1 cut(s) | 3298 |
| XmiI | GTMKAC | 2 cut(s) | 1104, 2985 |
| XmnI | GAANNNNTTC | 4 cut(s) | 14, 2039, 2530, 2951 |
| XspI | CTAG | 7 cut(s) | 147, 1157, 1868, 2199, 2792, 3050, 3652 |
| Zsp2I | ATGCAT | 3 cut(s) | 1120, 1710, 2080 |