Prupe.2G032800_v2.0.a1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000257
Physical Location & Seq
Reverse (-)
3541243 .. 3543423
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G032800.1

Sequence Viewer

Length: 2181 bp
ATGGCTAAGCATGGAGCTCTCCTGTTTTCTTATGCTTTCTCCATTCTCGTTTTCACTACGAGCTTATCGTGCAAAGCCATTGATTCGGAAGATAGAAAGACATATATTGTGTACCTGGGGTCACTTCCTCATGATGAGGTGTTCTCCCCATTGTCTAACCAAATTGGTATACTAGAAAGAGTCGTACAAAAAACTTCTGCCGCAAATTTCTTGCTCACAAGCTACAAAAGGAGCTTCAATGGATTTGCTGCCAAGCTCACTGAGCAAGAAAGAGAAAGGCTTGCTGGCATGAAGGAAGTTGTCTCTGTCTTTCCAAGCAGAACATTCCAACTTCAAACAACAAGATCTTGGGACTTTCTTGGTCTCAATCAGACAACCAAACGAAACGCTACTGTCGAGAGTAATACTATTATTGGTGTGATTGACAGTGGAATCTCGCCTGACTCAGAGAGCTTTAATGATGAAGGTTTTGGTCCAGCTCCCAAGAAGTGGAAAGGTGTTTGTAAAGGTGGCCAAAATTTCACTTGCAACAACAAGATCATTGGAGCTCGGTATTACACTGATGATGGCGCAAGTGATGCTGTTGGTCATGGAACTCACACTGCCTCAACCGCTGCAGGAAATCCCGTAAAGGATGTGAGCTTTTATGGACTAGCACAAGGTACAGCAAGAGGTGGAGTTCCCTCGGCGAGAATTGCTGCATATAAAGTCTGCAGTGTTTCAGGTTGCCCTACAGAGGCGATATTGCAGGGTTTTGATGATGCTATTGCCGATGGAGTTGACATCATTACAATTTCAATTGGAGCCGAAAGTTCAGCTCCTTTCCAGCAGGATCCTATAGCAATCGGTGCTTTTCATGCAATGGAGAAAGGTATACTAACCTTACAATCTGCAGGCAACAGTGGTCCTGAAGCAGGCAGCGTATCAAGTGTGGCACCATGGACGCTCACGGTTGCAGCGAGTAGCACAGATCGTCGAATCATTGACAAGATTGTTCTTGGAAATGGAAAGACAATTGTTGGGAGTTCAGTAAACTCTTTCAAGTTAAATGGAACAAATTTTCCGTTGGTATATGGAAAAGATGCTTCAAGTCAATGCGTTGACTCCGATGCAAGGCAATGTGTAGCCGGTTGCCTAGACGCCGATTTAGTTAAGGGAAAGATTGTGCTATGTGATCAGGCTGGTGGCAATACTGAGGCTCATCAAGCTGGTGCACTAGGTTCAATTTTGAACACTTCCAAACCTGATGTTGCTTTTGTTGTCCCACTACCTGCATCTGGTTTAGGCAGCCAAGACTATGATGTGGTCAAGTCCTACCTCAAATCCACCAAACGTCCTCGAGCCAACATACTAAAAAGTGAAGCCATAAAAGATGATGGTGCACCTGTTGTTGCTTCCTTCTCTTCACGCGGACCTAATCAAATTGTACCTGAAATTATTAAGCCCGATATAAGTGCCCCAGGGATAGATATTTTGGCTGCGTATTCGACTCTTGCTCCTATCACAGGTAGCACTGAAGACAAAAGGCGTGTAAAATACAGTATACTATCTGGAACCTCCATGTCTTGCCCGCATGTTGCTGGTGTAGCTGCATATATTAAGACATTCCACCCTGACTGGTCTCCAGCAGCCATCAAATCATCCATCATGACTACTGCTTGGCCCGTGAATGATACCAAAACTTCCCCTGCTGAATTTGCTTATGGATCTGGACATATCAATCCTCTAAAGGCTATTAACCCCGGCCTTGTGTTTGAAGCTTCTAAAGAGGATTACATTAAGTTTCTCTGCTCGGTCTTGGATGAGGGGAGCGTTAGACTTATATCCGGAGATAGCAGCTCTTGCCCTGCAGGGTCCGCCAAAGTATTGCCAAAGGATCTCAATTACCCTTCACTGGCAGCCAATGTTAATTCAAGCACTTCATTTACGATCAATTTTCTTAGAACAGTTAAAAATGTTGGCCTTCCAAACTCCACTTACAAGGCAAATATCCTCTCAAGTTCCGAAGTTGACATCAAAGTTGAGCCTGATGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGACCTTCGATGTGACCGTGGTTGGAAGAGGCATACCGGAAGGATCACATGTATCTGCGTCGCTGGTCTGGACGGATGGTACTCATAGCGTTAGAAGTCCAATTCTTGTAGCAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

727

Amino Acids

76.55

Weight (kDa)

6.62

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1279
AccB1I GGYRCC 1 cut(s) 934
AccB7I CCANNNNNTGG 1 cut(s) 489
AccI GTMKAC 3 cut(s) 169, 874, 1543
AccII CGCG 1 cut(s) 1410
AccIII TCCGGA 1 cut(s) 1826
AciI CCGC 5 cut(s) 201, 612, 1410, 1571, 1857
AclWI GGATC 5 cut(s) 827, 840, 1714, 1884, 2113
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 4 cut(s) 205, 517, 1057, 1694
AcuI CTGAAG 2 cut(s) 930, 1536
AcyI GRCGYC 1 cut(s) 1140
AfaI GTAC 5 cut(s) 113, 186, 664, 1428, 2143
AfiI CCNNNNNNNGG 7 cut(s) 489, 736, 914, 1113, 1277, 1505, 1894
AflIII ACRYGT 1 cut(s) 2110
AjnI CCWGG 2 cut(s) 114, 1459
AloI GAACNNNNNNTCC 2 cut(s) 1045, 1077
Alw21I GWGCWC 4 cut(s) 19, 550, 1216, 1384
Alw26I GTCTC 3 cut(s) 307, 368, 1626
Alw44I GTGCAC 2 cut(s) 1212, 1380
AlwI GGATC 5 cut(s) 827, 840, 1714, 1884, 2113
Ama87I CYCGRG 1 cut(s) 1338
Aor13HI TCCGGA 1 cut(s) 1826
AoxI GGCC 4 cut(s) 511, 1661, 1744, 1960
ApaLI GTGCAC 2 cut(s) 1212, 1380
ApoI RAATTY 4 cut(s) 205, 517, 1057, 1694
Asp700I GAANNNNTTC 1 cut(s) 2066
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 5 cut(s) 473, 905, 1412, 1662, 1854
AsuC2I CCSGG 1 cut(s) 1743
AvaI CYCGRG 1 cut(s) 1338
AvaII GGWCC 4 cut(s) 473, 905, 1412, 1854
BaeGI GKGCMC 3 cut(s) 1216, 1384, 1459
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 2 cut(s) 19, 550
BbsI GAAGAC 2 cut(s) 1524, 2069
Bbv12I GWGCWC 4 cut(s) 19, 550, 1216, 1384
BccI CCATC 6 cut(s) 560, 767, 1370, 1640, 1652, 2132
BcgI CGANNNNNNTGC 2 cut(s) 1436, 1470
BciT130I CCWGG 2 cut(s) 116, 1461
BclI TGATCA 1 cut(s) 1174
BcnI CCSGG 1 cut(s) 1743
BcoDI GTCTC 3 cut(s) 307, 368, 1626
BfaI CTAG 4 cut(s) 173, 653, 1136, 1217
BfmI CTRYAG 6 cut(s) 615, 712, 732, 837, 891, 1848
BfuAI ACCTGC 1 cut(s) 1279
BglII AGATCT 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 6
Bme1390I CCNGG 3 cut(s) 116, 1461, 1743
Bme18I GGWCC 4 cut(s) 473, 905, 1412, 1854
BmeT110I CYCGRG 1 cut(s) 1338
BmgT120I GGNCC 5 cut(s) 473, 905, 1412, 1662, 1854
BmiI GGNNCC 5 cut(s) 805, 834, 936, 1555, 1855
BmrFI CCNGG 3 cut(s) 116, 1461, 1743
BmsI GCATC 5 cut(s) 568, 751, 1072, 1099, 1283
BpiI GAAGAC 2 cut(s) 1524, 2069
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 1 cut(s) 1608
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 1981
BpuMI CCSGG 1 cut(s) 1743
BsaHI GRCGYC 1 cut(s) 1140
BsaI GGTCTC 2 cut(s) 368, 1626
BsaJI CCNNGG 7 cut(s) 115, 684, 938, 1459, 1460, 1741, 2079
BsaWI WCCGGW 2 cut(s) 1826, 2098
BsaXI ACNNNNNCTCC 2 cut(s) 1480, 1510
Bsc4I CCNNNNNNNGG 7 cut(s) 489, 736, 914, 1113, 1277, 1505, 1894
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 2 cut(s) 1622, 1899
Bse3DI GCAATG 2 cut(s) 867, 1124
BseAI TCCGGA 1 cut(s) 1826
BseBI CCWGG 2 cut(s) 116, 1461
BseDI CCNNGG 7 cut(s) 115, 684, 938, 1459, 1460, 1741, 2079
BseGI GGATG 4 cut(s) 640, 1640, 1807, 2143
BseLI CCNNNNNNNGG 7 cut(s) 489, 736, 914, 1113, 1277, 1505, 1894
BseMI GCAATG 2 cut(s) 867, 1124
BseMII CTCAG 3 cut(s) 252, 459, 1185
BseNI ACTGG 2 cut(s) 1622, 1899
BseRI GAGGAG 1 cut(s) 2072
BseSI GKGCMC 3 cut(s) 1216, 1384, 1459
Bsh1236I CGCG 1 cut(s) 1410
BshFI GGCC 4 cut(s) 513, 1663, 1746, 1962
BshNI GGYRCC 1 cut(s) 934
BsiHKAI GWGCWC 4 cut(s) 19, 550, 1216, 1384
BsiHKCI CYCGRG 1 cut(s) 1338
BsiSI CCGG 4 cut(s) 1128, 1743, 1827, 2099
BslFI GGGAC 2 cut(s) 365, 1247
BslI CCNNNNNNNGG 7 cut(s) 489, 736, 914, 1113, 1277, 1505, 1894
BsmAI GTCTC 3 cut(s) 307, 368, 1626
BsmFI GGGAC 2 cut(s) 365, 1247
BsnI GGCC 4 cut(s) 513, 1663, 1746, 1962
Bso31I GGTCTC 2 cut(s) 368, 1626
BsoBI CYCGRG 1 cut(s) 1338
Bsp1286I GDGCHC 5 cut(s) 19, 550, 1216, 1384, 1459
Bsp13I TCCGGA 1 cut(s) 1826
Bsp143I GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 5 cut(s) 201, 612, 1410, 1571, 1857
BspANI GGCC 4 cut(s) 513, 1663, 1746, 1962
BspCNI CTCAG 3 cut(s) 253, 458, 1186
BspEI TCCGGA 1 cut(s) 1826
BspFNI CGCG 1 cut(s) 1410
BspHI TCATGA 2 cut(s) 130, 1647
BspLI GGNNCC 5 cut(s) 805, 834, 936, 1555, 1855
BspMAI CTGCAG 4 cut(s) 619, 716, 895, 1852
BspMI ACCTGC 1 cut(s) 1279
BspPI GGATC 5 cut(s) 827, 840, 1714, 1884, 2113
BspT107I GGYRCC 1 cut(s) 934
BspTNI GGTCTC 2 cut(s) 368, 1626
BsrDI GCAATG 2 cut(s) 867, 1124
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 2 cut(s) 1622, 1899
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 7 cut(s) 115, 684, 938, 1459, 1460, 1741, 2079
BssMI GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
BssNAI GTATAC 3 cut(s) 170, 875, 1544
BssNI GRCGYC 1 cut(s) 1140
BssT1I CCWWGG 1 cut(s) 938
Bst1107I GTATAC 3 cut(s) 170, 875, 1544
Bst2UI CCWGG 2 cut(s) 116, 1461
Bst4CI ACNGT 7 cut(s) 394, 428, 902, 952, 1541, 1948, 2080
Bst6I CTCTTC 2 cut(s) 1408, 2083
BstACI GRCGYC 1 cut(s) 1140
BstAPI GCANNNNNTGC 3 cut(s) 578, 848, 1842
BstC8I GCNNGC 5 cut(s) 282, 286, 895, 916, 1571
BstDEI CTNAG 5 cut(s) 6, 261, 445, 1194, 1940
BstDSI CCRYGG 2 cut(s) 938, 2079
BstENI CCTNNNNNAGG 2 cut(s) 912, 1503
BstF5I GGATG 4 cut(s) 640, 1640, 1807, 2143
BstFNI CGCG 1 cut(s) 1410
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 9 cut(s) 347, 540, 835, 973, 1177, 1709, 1879, 1932, 2108
BstMAI GTCTC 3 cut(s) 307, 368, 1626
BstMBI GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
BstNI CCWGG 2 cut(s) 116, 1461
BstNSI RCATGY 2 cut(s) 1577, 2114
BstSCI CCNGG 3 cut(s) 114, 1459, 1741
BstSFI CTRYAG 6 cut(s) 615, 712, 732, 837, 891, 1848
BstSLI GKGCMC 3 cut(s) 1216, 1384, 1459
BstUI CGCG 1 cut(s) 1410
BstV2I GAAGAC 2 cut(s) 1524, 2069
BstX2I RGATCY 4 cut(s) 344, 832, 1706, 1876
BstYI RGATCY 4 cut(s) 344, 832, 1706, 1876
BstZ17I GTATAC 3 cut(s) 170, 875, 1544
BsuRI GGCC 4 cut(s) 513, 1663, 1746, 1962
BtgI CCRYGG 2 cut(s) 938, 2079
BtsCI GGATG 4 cut(s) 640, 1640, 1807, 2143
BtsI GCAGTG 2 cut(s) 600, 721
BtsIMutI CAGTG 8 cut(s) 258, 433, 558, 600, 721, 907, 1512, 1892
BveI ACCTGC 1 cut(s) 1279
Cac8I GCNNGC 5 cut(s) 282, 286, 895, 916, 1571
CciI TCATGA 2 cut(s) 130, 1647
CfoI GCGC 1 cut(s) 572
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 5 cut(s) 473, 905, 1412, 1662, 1854
CseI GACGC 3 cut(s) 952, 1148, 2109
Csp6I GTAC 5 cut(s) 112, 185, 663, 1427, 2142
CviQI GTAC 5 cut(s) 112, 185, 663, 1427, 2142
DdeI CTNAG 5 cut(s) 6, 261, 445, 1194, 1940
DpnI GATC 9 cut(s) 346, 539, 834, 972, 1176, 1708, 1878, 1931, 2107
DpnII GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 2 cut(s) 1408, 2083
EarI CTCTTC 2 cut(s) 1408, 2083
EciI GGCGGA 1 cut(s) 1846
Ecl136II GAGCTC 2 cut(s) 17, 548
Eco130I CCWWGG 1 cut(s) 938
Eco24I GRGCYC 2 cut(s) 19, 550
Eco31I GGTCTC 2 cut(s) 368, 1626
Eco47I GGWCC 4 cut(s) 473, 905, 1412, 1854
Eco53kI GAGCTC 2 cut(s) 17, 548
Eco57I CTGAAG 2 cut(s) 930, 1536
Eco88I CYCGRG 1 cut(s) 1338
EcoICRI GAGCTC 2 cut(s) 17, 548
EcoNI CCTNNNNNAGG 2 cut(s) 912, 1503
EcoRII CCWGG 2 cut(s) 114, 1459
EcoT14I CCWWGG 1 cut(s) 938
EcoT38I GRGCYC 2 cut(s) 19, 550
ErhI CCWWGG 1 cut(s) 938
FaqI GGGAC 2 cut(s) 365, 1247
FauI CCCGC 1 cut(s) 1578
FbaI TGATCA 1 cut(s) 1174
FblI GTMKAC 3 cut(s) 169, 874, 1543
FokI GGATG 4 cut(s) 647, 1627, 1814, 2150
FriOI GRGCYC 2 cut(s) 19, 550
FspBI CTAG 4 cut(s) 173, 653, 1136, 1217
GlaI GCGC 1 cut(s) 571
GsuI CTGGAG 1 cut(s) 1608
HaeIII GGCC 4 cut(s) 513, 1663, 1746, 1962
HapII CCGG 4 cut(s) 1128, 1743, 1827, 2099
HgaI GACGC 3 cut(s) 952, 1148, 2109
HhaI GCGC 1 cut(s) 572
Hin1I GRCGYC 1 cut(s) 1140
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 3 cut(s) 781, 1102, 2011
HindII GTYRAC 3 cut(s) 781, 1102, 2011
HindIII AAGCTT 1 cut(s) 1758
HinfI GANTC 7 cut(s) 83, 180, 432, 443, 978, 1103, 1489
HpaII CCGG 4 cut(s) 1128, 1743, 1827, 2099
Hpy188I TCNGA 5 cut(s) 88, 372, 448, 1108, 2005
Hpy188III TCNNGA 8 cut(s) 131, 397, 908, 1551, 1648, 1710, 1827, 2131
Hpy99I CGWCG 2 cut(s) 978, 2125
HpyAV CCTTC 8 cut(s) 286, 458, 1408, 1899, 1973, 2050, 2077, 2096
HpyCH4III ACNGT 7 cut(s) 394, 428, 902, 952, 1541, 1948, 2080
HpyCH4IV ACGT 1 cut(s) 1333
HpyF3I CTNAG 5 cut(s) 6, 261, 445, 1194, 1940
HpySE526I ACGT 1 cut(s) 1333
Hsp92I GRCGYC 1 cut(s) 1140
HspAI GCGC 1 cut(s) 570
Kpn2I TCCGGA 1 cut(s) 1826
Ksp22I TGATCA 1 cut(s) 1174
Kzo9I GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
LmnI GCTCC 8 cut(s) 14, 231, 484, 545, 803, 823, 1501, 1809
LweI GCATC 5 cut(s) 568, 751, 1072, 1099, 1283
MaeI CTAG 4 cut(s) 173, 653, 1136, 1217
MaeII ACGT 1 cut(s) 1333
MaeIII GTNAC 2 cut(s) 120, 2074
MalI GATC 9 cut(s) 346, 539, 834, 972, 1176, 1708, 1878, 1931, 2107
MboI GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
MboII GAAGA 5 cut(s) 101, 1395, 1529, 2074, 2100
MfeI CAATTG 2 cut(s) 798, 1014
MflI RGATCY 4 cut(s) 344, 832, 1706, 1876
MhlI GDGCHC 5 cut(s) 19, 550, 1216, 1384, 1459
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 4 cut(s) 189, 437, 1097, 1483
MmeI TCCRAC 2 cut(s) 352, 2065
Mox20I TGGCCA 1 cut(s) 513
MroI TCCGGA 1 cut(s) 1826
MroXI GAANNNNTTC 1 cut(s) 2066
MscI TGGCCA 1 cut(s) 513
MseI TTAA 9 cut(s) 456, 1046, 1152, 1440, 1599, 1737, 1779, 1908, 1950
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 4 cut(s) 1128, 1743, 1827, 2099
MspR9I CCNGG 3 cut(s) 116, 1461, 1743
MunI CAATTG 2 cut(s) 798, 1014
MvaI CCWGG 2 cut(s) 116, 1461
MvnI CGCG 1 cut(s) 1410
NciI CCSGG 1 cut(s) 1743
NcoI CCATGG 1 cut(s) 938
NdeII GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
NlaIV GGNNCC 5 cut(s) 805, 834, 936, 1555, 1855
NmeAIII GCCGAG 1 cut(s) 665
NmuCI GTSAC 2 cut(s) 120, 2074
NspI RCATGY 2 cut(s) 1577, 2114
PaeR7I CTCGAG 1 cut(s) 1338
PagI TCATGA 2 cut(s) 130, 1647
PasI CCCWGGG 1 cut(s) 1460
PciI ACATGT 1 cut(s) 2110
PcsI WCGNNNNNNNCGW 4 cut(s) 65, 393, 956, 2076
PdmI GAANNNNTTC 1 cut(s) 2066
PfeI GAWTC 3 cut(s) 83, 432, 978
PflMI CCANNNNNTGG 1 cut(s) 489
PleI GAGTC 4 cut(s) 188, 437, 1097, 1483
PpsI GAGTC 4 cut(s) 188, 437, 1097, 1483
PscI ACATGT 1 cut(s) 2110
Psp124BI GAGCTC 2 cut(s) 19, 550
Psp6I CCWGG 2 cut(s) 114, 1459
PspGI CCWGG 2 cut(s) 114, 1459
PspN4I GGNNCC 5 cut(s) 805, 834, 936, 1555, 1855
PspPI GGNCC 5 cut(s) 473, 905, 1412, 1662, 1854
PspXI VCTCGAGB 1 cut(s) 1338
PstI CTGCAG 4 cut(s) 619, 716, 895, 1852
PsuI RGATCY 4 cut(s) 344, 832, 1706, 1876
RsaI GTAC 5 cut(s) 113, 186, 664, 1428, 2143
RsaNI GTAC 5 cut(s) 112, 185, 663, 1427, 2142
SacI GAGCTC 2 cut(s) 19, 550
SaqAI TTAA 9 cut(s) 456, 1046, 1152, 1440, 1599, 1737, 1779, 1908, 1950
Sau3AI GATC 9 cut(s) 344, 537, 832, 970, 1174, 1706, 1876, 1929, 2105
Sau96I GGNCC 5 cut(s) 473, 905, 1412, 1662, 1854
SbfI CCTGCAGG 1 cut(s) 1852
SchI GAGTC 4 cut(s) 189, 437, 1097, 1483
ScrFI CCNGG 3 cut(s) 116, 1461, 1743
SdaI CCTGCAGG 1 cut(s) 1852
SduI GDGCHC 5 cut(s) 19, 550, 1216, 1384, 1459
SfaNI GCATC 5 cut(s) 568, 751, 1072, 1099, 1283
SfcI CTRYAG 6 cut(s) 615, 712, 732, 837, 891, 1848
Sfr274I CTCGAG 1 cut(s) 1338
SinI GGWCC 4 cut(s) 473, 905, 1412, 1854
SlaI CTCGAG 1 cut(s) 1338
SmlI CTYRAG 2 cut(s) 1338, 1996
SmoI CTYRAG 2 cut(s) 1338, 1996
Sse8387I CCTGCAGG 1 cut(s) 1852
SsiI CCGC 5 cut(s) 201, 612, 1410, 1571, 1857
SspMI CTAG 4 cut(s) 173, 653, 1136, 1217
SstI GAGCTC 2 cut(s) 19, 550
StyD4I CCNGG 3 cut(s) 114, 1459, 1741
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 7 cut(s) 394, 428, 902, 952, 1541, 1948, 2080
TaiI ACGT 1 cut(s) 1336
TaqI TCGA 5 cut(s) 396, 976, 1339, 1487, 2070
TaqII GACCGA 1 cut(s) 1783
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 3 cut(s) 83, 432, 978
Tru1I TTAA 9 cut(s) 456, 1046, 1152, 1440, 1599, 1737, 1779, 1908, 1950
Tru9I TTAA 9 cut(s) 456, 1046, 1152, 1440, 1599, 1737, 1779, 1908, 1950
TscAI CASTG 8 cut(s) 265, 433, 565, 607, 721, 907, 1519, 1899
TseFI GTSAC 2 cut(s) 120, 2074
Tsp45I GTSAC 2 cut(s) 120, 2074
TspDTI ATGAA 4 cut(s) 305, 477, 845, 1911
TspGWI ACGGA 2 cut(s) 1053, 2150
TspRI CASTG 8 cut(s) 265, 433, 565, 607, 721, 907, 1519, 1899
Van91I CCANNNNNTGG 1 cut(s) 489
VneI GTGCAC 2 cut(s) 1212, 1380
VpaK11BI GGWCC 4 cut(s) 473, 905, 1412, 1854
XagI CCTNNNNNAGG 2 cut(s) 912, 1503
XapI RAATTY 4 cut(s) 205, 517, 1057, 1694
XceI RCATGY 2 cut(s) 1577, 2114
XhoI CTCGAG 1 cut(s) 1338
XmiI GTMKAC 3 cut(s) 169, 874, 1543
XmnI GAANNNNTTC 1 cut(s) 2066
XspI CTAG 4 cut(s) 173, 653, 1136, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.