Rroxscaffold_4G00326650

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000257
Physical Location & Seq
Forward (+)
58808083 .. 58817372
9290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326650.1

Sequence Viewer

Length: 3240 bp
ATGGCTAAGCATGTAGCTATCCTATTTTCTTATGCTTTCCCCACTCTTATACTAACCTTCAACTTCTTCCTGTGCAAAGCCATTGATGAAAACAGAACGGTCCACATTGTGTACCTTGGTTCACTTCCTGATAATGAGGTGTACTCACCAATGTCTCACCACCTTCGATTACTCCAAAAAGTTGTCGACAGCAACTCCGCAGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCAAAGCTCACTGACCGGGAAAGGAAAAGGCTTGCTAACATGAAGGAAGTAGTCTCTGTATTTCCAAGTAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCCATGAGAAAATAAAACGGAATGTCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGACACTGGAATTTGGCCTGAATCAGAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGAAGGTGGCCAAAATTTCACTTGCAACAAGAAGCTAATTGGAGCTCGGTTTTACAATTCATCTTCAGAATCTGCAAGGGATGCACAAGGCCATGGAACCCACACAGCCTCAACTGCAGCAGGGAACGCTATAAAGGATGTGAGCTTTTATGGACTAGCACAAGGTACTGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTATGTGGTGTTCTTGGGTGCCCTACTGATGCTATATTGGCTGCTTTTGATGATGCTATTGCCGATGGAGTTGACATCATTACAGTTTCATTAGGAAACCCGGTTGCATCTGTATTACAGCATGACCCTATTGCTATCGGGGCTTTTCATGCAATGGCAAAGGGGATACTTACATCAAATTCTGCAGGCAATGGTGGTCCTGTTGATTCAAGTGTTTCAAGTGTAGCACCATGGATACTTACAGTTGCAGCAAGTAGTACTGATCGTAGGATCATTGACAAGGTTGTTCTCGAAAATGGAACTATGATGGTTGGGGCTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTACTTGCTCCGAGTTTGAAGCTGGGAGATGTTCATACGGCTGCTTAGATAGTGGTTTAGTTAAGGGAAAGGTTGTGTTATGTGACATGCCTAACAGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTCATTTTGAGAGTTGATTATGATGATGTTTCTCAAATTGTACCCTTTCCTACAACAGCTTTTGCAGGCAAAGAGCATAGTCTGATAAAGTTGTACACGAACTCCACGAAAGATCCTCGAGTAAACATACTAAGAAGTGAAGAAGAAAGGGATACAGACGCACCTGTTGTTGCTTCCTTCTCTTCACGTGGACCAAATTTAATTCTACCAGAAATTATCAAGCCAGATATAAGCGCCCCAGGGGTTACTATTTTGGCTGCCTTTTCACCTATTGCTTCAGTTGCAAAGAGTCTTCTAGACACGAGGCATGTCAAATATAGTATACTATCTGGAACCTCTATGTCTTGTCCGCACGCGGCTGGTGCAGCTGCATATGTTAAAGCATTCCATCCTAATTGGTCTCCAGCATCCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATGTTACTGATAATAGCAGTGCTGCTGGTGAATTTGCTTATGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACGTAAAGTTGCTATGCTCAGTCTTGGATCAGGCCGATGTTAGACTTATATCAGGAGATAACAACACTTGCTCTACAGGCTCTGACAAAGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCCATTGTCACACCAAACAAATCTTTTTCATTCAAATTTCACAGAAAAGTTAAGAATGTTGGCCTTGCAAACTCCACTTACAAGGCCACAATATTCGCCAACTCTACTCAAGTTGACGTCAAAGTAGTGCCTCAAGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTGATGTGACAGTTGCCGGAAAAGGTTTGCCAAATGAAGTACAATCACATGTGTCTGCATCGCTGGTGTGGTCTGATGGAATTCACAACGTTAGAAGGTACTCACCATTGTCTCACCATCTTGGTATACTAGAAAGAGTTGTCGAGAGCAACTCCACTTCCAATTTTCTTACAAGAAGTTACCAAAGGAGTTTCAATGGATTTGCTGCATACCTCACTGACCGGGAAAGGAAAAGGCTTGCTAGCATGAAAGAAGTAGTCTCTGTATTTCCAAGCACGACTTTCCAACTTCAAACGACAAGATCTTGGGACTTTCTAGGATTCAATGAGAAAATCCAACGAAATGACAGCGTTGAGAGTGATATCATTATTGGTGTCATCGACTCTGAAATTTGGCCTGATTCGGAGAGCTTCAAAGACGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGTAGGTGGAAAAAAATTTACTTGTAATAATAAGCTCATTGGTGCTCGGTTTTACAACTCATCCTCCGAGTCTGCAAGGGATGAAAGTGGCCATGGAACACACACTGCCTCAATAGCATCCGGGAATGCTGTAAAAGATGTCAGCTTTTATGGTATAGCCCAAGGCATTGCAAGAGGGGGAGTTCCCTCAGCGAGAGTTGCTGCGTATAGAGTCTGCGGTGACCAAGGATGCCCTACAGAGGCTATCTTGGCTGCTTTTGACGATGCTATTGCCGATGGAGTTGATATCATTACAATTTCAATAGGAAGCCCGGATAAATGGAGGTTATTACATACATCAAACTCAGCAGGCAATAATGGTCCTCGAGCTAGTACCGTAACAAGTGTAGCACCATGGTTATTTACAGTTGCAGCAAGTACTACAGATCGTAGAATCATTGACAAGGTTGTTCTTGGAAATGGTACAACAGTAGTTGGGGCTTCAGTGAACTCTTTCCAAATGACTGGGACAAGTTTTCCATTAATATATGGAAAAGATGCTACAACTCAATGCCTAGAGTTTGATGCCCGGAAGTGTATACAAGACTGCTTAGACCGTGGTTTGGTTAAGGGAAAGGTTGTGTTATGTGACGAGTCATATGGAATTGATCGATGGGGTCTATCAATCTGGATCACTGGGTTCAATTTTGAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1079

Amino Acids

116.03

Weight (kDa)

7.94

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 110 1.3e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 575 8.2e-44 Subtilase family
fn3_6 PF17766 630 - 726 7.5e-21 Fibronectin type-III domain
Inhibitor_I9 PF05922 732 - 792 1.7e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 814 - 967 9.7e-15 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2031
AccB1I GGYRCC 1 cut(s) 732
AccB7I CCANNNNNTGG 2 cut(s) 489, 2529
AccI GTMKAC 4 cut(s) 186, 1549, 2209, 3121
AccII CGCG 1 cut(s) 1583
AciI CCGC 4 cut(s) 198, 1577, 1583, 2760
AclI AACGTT 1 cut(s) 2172
AclWI GGATC 7 cut(s) 992, 1334, 1726, 1821, 1885, 1896, 3221
AcoI YGGCCR 2 cut(s) 511, 2632
AcuI CTGAAG 4 cut(s) 552, 1017, 1488, 3009
AcvI CACGTG 1 cut(s) 1415
AcyI GRCGYC 1 cut(s) 2028
AdeI CACNNNGTG 1 cut(s) 109
AfiI CCNNNNNNNGG 4 cut(s) 489, 2529, 2782, 3145
AflIII ACRYGT 1 cut(s) 2131
AjnI CCWGG 2 cut(s) 1465, 1753
Alw21I GWGCWC 2 cut(s) 550, 2590
Alw26I GTCTC 5 cut(s) 159, 307, 1632, 2199, 2347
AlwI GGATC 7 cut(s) 992, 1334, 1726, 1821, 1885, 1896, 3221
AlwNI CAGNNNCTG 2 cut(s) 575, 1868
Ama87I CYCGRG 2 cut(s) 1344, 2907
AoxI GGCC 9 cut(s) 437, 511, 592, 1203, 1818, 1972, 1995, 2477, 2632
ArsI GACNNNNNNTTYG 2 cut(s) 2492, 2524
AseI ATTAAT 1 cut(s) 3065
Asp700I GAANNNNTTC 2 cut(s) 1043, 3035
AspLEI GCGC 1 cut(s) 1463
AspS9I GGNCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
AsuC2I CCSGG 6 cut(s) 266, 815, 2306, 2665, 2855, 3112
AsuHPI GGTGA 7 cut(s) 138, 149, 1485, 1715, 2178, 2189, 2774
AsuNHI GCTAGC 1 cut(s) 2324
AvaI CYCGRG 2 cut(s) 1344, 2907
AvaII GGWCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
BaeGI GKGCMC 1 cut(s) 737
BaeI ACNNNNGTAYC 2 cut(s) 2997, 3030
BalI TGGCCA 2 cut(s) 513, 2634
BanI GGYRCC 1 cut(s) 732
BanII GRGCYC 1 cut(s) 550
BauI CACGAG 1 cut(s) 1528
BbrPI CACGTG 1 cut(s) 1415
BbsI GAAGAC 3 cut(s) 1511, 1788, 2084
Bbv12I GWGCWC 2 cut(s) 550, 2590
BbvCI CCTCAGC 2 cut(s) 691, 2731
BceAI ACGGC 1 cut(s) 1150
BcgI CGANNNNNNTGC 2 cut(s) 2795, 2829
BciT130I CCWGG 2 cut(s) 1467, 1755
BciVI GTATCC 3 cut(s) 873, 942, 1372
BcnI CCSGG 6 cut(s) 266, 815, 2306, 2665, 2855, 3112
BcoDI GTCTC 5 cut(s) 159, 307, 1632, 2199, 2347
BfaI CTAG 8 cut(s) 659, 1523, 1907, 2213, 2325, 2399, 2913, 3098
BfmI CTRYAG 7 cut(s) 618, 897, 1659, 1746, 1860, 2778, 2964
BfoI RGCGCY 1 cut(s) 1464
BfuI GTATCC 3 cut(s) 873, 942, 1372
BglII AGATCT 2 cut(s) 344, 2384
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 3 cut(s) 973, 1099, 2962
Bme1390I CCNGG 8 cut(s) 266, 815, 1467, 1755, 2306, 2665, 2855, 3112
Bme18I GGWCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
BmeT110I CYCGRG 2 cut(s) 1344, 2907
BmgT120I GGNCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
BmiI GGNNCC 4 cut(s) 601, 687, 734, 1561
BmrFI CCNGG 8 cut(s) 266, 815, 1467, 1755, 2306, 2665, 2855, 3112
BmrI ACTGGG 2 cut(s) 3057, 3228
BmtI GCTAGC 1 cut(s) 2328
BmuI ACTGGG 2 cut(s) 3057, 3228
BpiI GAAGAC 3 cut(s) 1511, 1788, 2084
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 2219, 2251
BpmI CTGGAG 2 cut(s) 1236, 1614
Bpu10I CCTNAGC 2 cut(s) 691, 2731
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 2004, 2028
BpuMI CCSGG 6 cut(s) 266, 815, 2306, 2665, 2855, 3112
Bsa29I ATCGAT 1 cut(s) 3193
BsaAI YACGTR 2 cut(s) 1415, 1789
BsaHI GRCGYC 1 cut(s) 2028
BsaI GGTCTC 1 cut(s) 1632
Bsc4I CCNNNNNNNGG 4 cut(s) 489, 2529, 2782, 3145
Bse1I ACTGG 3 cut(s) 433, 3052, 3223
Bse3DI GCAATG 3 cut(s) 873, 910, 2709
BseBI CCWGG 2 cut(s) 1467, 1755
BseCI ATCGAT 1 cut(s) 3193
BseGI GGATG 9 cut(s) 328, 589, 646, 1615, 1634, 2603, 2629, 2660, 2777
BseLI CCNNNNNNNGG 4 cut(s) 489, 2529, 2782, 3145
BseMI GCAATG 3 cut(s) 873, 910, 2709
BseMII CTCAG 5 cut(s) 347, 705, 1818, 2745, 2901
BseNI ACTGG 3 cut(s) 433, 3052, 3223
BseRI GAGGAG 4 cut(s) 468, 693, 2087, 2508
BseSI GKGCMC 1 cut(s) 737
BseYI CCCAGC 1 cut(s) 1118
BsgI GTGCAG 1 cut(s) 1611
Bsh1236I CGCG 1 cut(s) 1583
BshFI GGCC 9 cut(s) 439, 513, 594, 1205, 1820, 1974, 1997, 2479, 2634
BshNI GGYRCC 1 cut(s) 732
BshVI ATCGAT 1 cut(s) 3193
BsiHKAI GWGCWC 2 cut(s) 550, 2590
BsiHKCI CYCGRG 2 cut(s) 1344, 2907
BsiSI CCGG 7 cut(s) 265, 815, 2100, 2305, 2664, 2855, 3112
BslFI GGGAC 4 cut(s) 365, 1072, 2405, 3064
BslI CCNNNNNNNGG 4 cut(s) 489, 2529, 2782, 3145
BsmAI GTCTC 5 cut(s) 159, 307, 1632, 2199, 2347
BsmFI GGGAC 4 cut(s) 365, 1072, 2405, 3064
BsmI GAATGC 2 cut(s) 1610, 2674
BsnI GGCC 9 cut(s) 439, 513, 594, 1205, 1820, 1974, 1997, 2479, 2634
Bso31I GGTCTC 1 cut(s) 1632
BsoBI CYCGRG 2 cut(s) 1344, 2907
Bsp1286I GDGCHC 3 cut(s) 550, 737, 2590
Bsp1407I TGTACA 1 cut(s) 1320
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 4 cut(s) 595, 944, 2635, 2936
BspACI CCGC 4 cut(s) 198, 1577, 1583, 2760
BspANI GGCC 9 cut(s) 439, 513, 594, 1205, 1820, 1974, 1997, 2479, 2634
BspCNI CTCAG 5 cut(s) 346, 704, 1817, 2744, 2900
BspDI ATCGAT 1 cut(s) 3193
BspFNI CGCG 1 cut(s) 1583
BspLI GGNNCC 4 cut(s) 601, 687, 734, 1561
BspMAI CTGCAG 2 cut(s) 622, 901
BspOI GCTAGC 1 cut(s) 2328
BspPI GGATC 7 cut(s) 992, 1334, 1726, 1821, 1885, 1896, 3221
BspT107I GGYRCC 1 cut(s) 732
BspTNI GGTCTC 1 cut(s) 1632
BsrDI GCAATG 3 cut(s) 873, 910, 2709
BsrGI TGTACA 1 cut(s) 1320
BsrI ACTGG 3 cut(s) 433, 3052, 3223
BssNAI GTATAC 3 cut(s) 1550, 2210, 3122
BssNI GRCGYC 1 cut(s) 2028
BssSI CACGAG 1 cut(s) 1528
BssT1I CCWWGG 7 cut(s) 115, 595, 944, 2635, 2704, 2767, 2936
Bst1107I GTATAC 3 cut(s) 1550, 2210, 3122
Bst2BI CACGAG 1 cut(s) 1528
Bst2UI CCWGG 2 cut(s) 1467, 1755
Bst4CI ACNGT 8 cut(s) 100, 799, 958, 2095, 2920, 2950, 3013, 3140
Bst6I CTCTTC 1 cut(s) 1414
BstACI GRCGYC 1 cut(s) 2028
BstAPI GCANNNNNTGC 1 cut(s) 584
BstAUI TGTACA 1 cut(s) 1320
BstBAI YACGTR 2 cut(s) 1415, 1789
BstC8I GCNNGC 7 cut(s) 282, 901, 1294, 1581, 2322, 2326, 2893
BstDSI CCRYGG 5 cut(s) 595, 944, 2635, 2936, 3139
BstEII GGTNACC 1 cut(s) 2762
BstF5I GGATG 9 cut(s) 328, 589, 646, 1615, 1634, 2603, 2629, 2660, 2777
BstFNI CGCG 1 cut(s) 1583
BstH2I RGCGCY 1 cut(s) 1464
BstHHI GCGC 1 cut(s) 1463
BstMAI GTCTC 5 cut(s) 159, 307, 1632, 2199, 2347
BstNI CCWGG 2 cut(s) 1467, 1755
BstNSI RCATGY 4 cut(s) 14, 1186, 1538, 2135
BstPI GGTNACC 1 cut(s) 2762
BstSCI CCNGG 8 cut(s) 264, 813, 1465, 1753, 2304, 2663, 2853, 3110
BstSFI CTRYAG 7 cut(s) 618, 897, 1659, 1746, 1860, 2778, 2964
BstSLI GKGCMC 1 cut(s) 737
BstSNI TACGTA 1 cut(s) 1789
BstUI CGCG 1 cut(s) 1583
BstV2I GAAGAC 3 cut(s) 1511, 1788, 2084
BstX2I RGATCY 5 cut(s) 344, 1339, 1718, 1877, 2384
BstXI CCANNNNNNTGG 1 cut(s) 3047
BstYI RGATCY 5 cut(s) 344, 1339, 1718, 1877, 2384
BstZ17I GTATAC 3 cut(s) 1550, 2210, 3122
Bsu15I ATCGAT 1 cut(s) 3193
BsuI GTATCC 3 cut(s) 873, 942, 1372
BsuRI GGCC 9 cut(s) 439, 513, 594, 1205, 1820, 1974, 1997, 2479, 2634
BsuTUI ATCGAT 1 cut(s) 3193
BtgI CCRYGG 5 cut(s) 595, 944, 2635, 2936, 3139
BtgZI GCGATG 1 cut(s) 2127
BtsCI GGATG 9 cut(s) 328, 589, 646, 1615, 1634, 2603, 2629, 2660, 2777
BtsI GCAGTG 2 cut(s) 1699, 2646
Cac8I GCNNGC 7 cut(s) 282, 901, 1294, 1581, 2322, 2326, 2893
CaiI CAGNNNCTG 2 cut(s) 575, 1868
CfoI GCGC 1 cut(s) 1463
Cfr13I GGNCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
ClaI ATCGAT 1 cut(s) 3193
CseI GACGC 1 cut(s) 1394
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 511, 2632
Eam1104I CTCTTC 1 cut(s) 1414
EarI CTCTTC 1 cut(s) 1414
Ecl136II GAGCTC 1 cut(s) 548
Eco105I TACGTA 1 cut(s) 1789
Eco130I CCWWGG 7 cut(s) 115, 595, 944, 2635, 2704, 2767, 2936
Eco147I AGGCCT 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 1632
Eco32I GATATC 2 cut(s) 2446, 2830
Eco47I GGWCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 4 cut(s) 552, 1017, 1488, 3009
Eco72I CACGTG 1 cut(s) 1415
Eco88I CYCGRG 2 cut(s) 1344, 2907
Eco91I GGTNACC 1 cut(s) 2762
EcoICRI GAGCTC 1 cut(s) 548
EcoO65I GGTNACC 1 cut(s) 2762
EcoRI GAATTC 2 cut(s) 321, 2163
EcoRII CCWGG 2 cut(s) 1465, 1753
EcoRV GATATC 2 cut(s) 2446, 2830
EcoT14I CCWWGG 7 cut(s) 115, 595, 944, 2635, 2704, 2767, 2936
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 7 cut(s) 115, 595, 944, 2635, 2704, 2767, 2936
FalI AAGNNNNNCTT 2 cut(s) 2347, 2379
FaqI GGGAC 4 cut(s) 365, 1072, 2405, 3064
FauNDI CATATG 2 cut(s) 1600, 3181
FblI GTMKAC 4 cut(s) 186, 1549, 2209, 3121
FokI GGATG 9 cut(s) 315, 596, 653, 1602, 1621, 2590, 2636, 2647, 2784
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 8 cut(s) 659, 1523, 1907, 2213, 2325, 2399, 2913, 3098
GlaI GCGC 1 cut(s) 1462
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 2 cut(s) 1236, 1614
HaeII RGCGCY 1 cut(s) 1464
HaeIII GGCC 9 cut(s) 439, 513, 594, 1205, 1820, 1974, 1997, 2479, 2634
HapII CCGG 7 cut(s) 265, 815, 2100, 2305, 2664, 2855, 3112
HgaI GACGC 1 cut(s) 1394
HhaI GCGC 1 cut(s) 1463
Hin1I GRCGYC 1 cut(s) 2028
Hin6I GCGC 1 cut(s) 1461
HinP1I GCGC 1 cut(s) 1461
HincII GTYRAC 3 cut(s) 187, 787, 2026
HindII GTYRAC 3 cut(s) 187, 787, 2026
HindIII AAGCTT 1 cut(s) 1770
HpaII CCGG 7 cut(s) 265, 815, 2100, 2305, 2664, 2855, 3112
HphI GGTGA 7 cut(s) 138, 149, 1485, 1715, 2178, 2189, 2774
HpyAV CCTTC 8 cut(s) 67, 173, 286, 500, 1414, 1911, 2065, 2172
HpyCH4III ACNGT 8 cut(s) 100, 799, 958, 2095, 2920, 2950, 3013, 3140
HpyCH4IV ACGT 4 cut(s) 1414, 1788, 2028, 2172
HpySE526I ACGT 4 cut(s) 1414, 1788, 2028, 2172
Hsp92I GRCGYC 1 cut(s) 2028
HspAI GCGC 1 cut(s) 1461
LmnI GCTCC 3 cut(s) 545, 1109, 1217
MaeI CTAG 8 cut(s) 659, 1523, 1907, 2213, 2325, 2399, 2913, 3098
MaeII ACGT 4 cut(s) 1414, 1788, 2028, 2172
MboII GAAGA 8 cut(s) 58, 558, 1379, 1382, 1401, 1511, 1793, 2089
MflI RGATCY 5 cut(s) 344, 1339, 1718, 1877, 2384
MhlI GDGCHC 3 cut(s) 550, 737, 2590
MlsI TGGCCA 2 cut(s) 513, 2634
MluNI TGGCCA 2 cut(s) 513, 2634
MlyI GAGTC 5 cut(s) 1525, 2459, 2621, 2763, 3185
MmeI TCCRAC 2 cut(s) 2392, 2443
Mox20I TGGCCA 2 cut(s) 513, 2634
MroXI GAANNNNTTC 2 cut(s) 1043, 3035
MscI TGGCCA 2 cut(s) 513, 2634
MseI TTAA 8 cut(s) 456, 1052, 1158, 1427, 1605, 1962, 3065, 3150
Msp20I TGGCCA 2 cut(s) 513, 2634
MspA1I CMGCKG 1 cut(s) 1595
MspI CCGG 7 cut(s) 265, 815, 2100, 2305, 2664, 2855, 3112
MspR9I CCNGG 8 cut(s) 266, 815, 1467, 1755, 2306, 2665, 2855, 3112
Mva1269I GAATGC 2 cut(s) 1610, 2674
MvaI CCWGG 2 cut(s) 1467, 1755
MvnI CGCG 1 cut(s) 1583
NciI CCSGG 6 cut(s) 266, 815, 2306, 2665, 2855, 3112
NcoI CCATGG 4 cut(s) 595, 944, 2635, 2936
NdeI CATATG 2 cut(s) 1600, 3181
NheI GCTAGC 1 cut(s) 2324
NlaIV GGNNCC 4 cut(s) 601, 687, 734, 1561
NmuCI GTSAC 5 cut(s) 1178, 1918, 2089, 2762, 3170
NspI RCATGY 4 cut(s) 14, 1186, 1538, 2135
PaeR7I CTCGAG 2 cut(s) 1344, 2907
PasI CCCWGGG 2 cut(s) 1466, 1754
PceI AGGCCT 1 cut(s) 1205
PciI ACATGT 1 cut(s) 2131
PcsI WCGNNNNNNNCGW 1 cut(s) 1331
PctI GAATGC 2 cut(s) 1610, 2674
PdmI GAANNNNTTC 2 cut(s) 1043, 3035
PfeI GAWTC 6 cut(s) 443, 572, 920, 2403, 2483, 2976
PflMI CCANNNNNTGG 2 cut(s) 489, 2529
PfoI TCCNGGA 1 cut(s) 2663
PleI GAGTC 5 cut(s) 1524, 2459, 2620, 2762, 3184
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
PpsI GAGTC 5 cut(s) 1524, 2459, 2620, 2762, 3184
Ppu21I YACGTR 2 cut(s) 1415, 1789
PscI ACATGT 1 cut(s) 2131
PshBI ATTAAT 1 cut(s) 3065
Psp124BI GAGCTC 1 cut(s) 550
Psp1406I AACGTT 1 cut(s) 2172
Psp6I CCWGG 2 cut(s) 1465, 1753
PspCI CACGTG 1 cut(s) 1415
PspEI GGTNACC 1 cut(s) 2762
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 2 cut(s) 1465, 1753
PspN4I GGNNCC 4 cut(s) 601, 687, 734, 1561
PspPI GGNCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
PspXI VCTCGAGB 2 cut(s) 1344, 2907
PsrI GAACNNNNNNTAC 2 cut(s) 708, 740
PstI CTGCAG 2 cut(s) 622, 901
PstNI CAGNNNCTG 2 cut(s) 575, 1868
PsuI RGATCY 5 cut(s) 344, 1339, 1718, 1877, 2384
PvuII CAGCTG 1 cut(s) 1595
SacI GAGCTC 1 cut(s) 550
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 8 cut(s) 456, 1052, 1158, 1427, 1605, 1962, 3065, 3150
Sau96I GGNCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
ScaI AGTACT 3 cut(s) 973, 1099, 2962
SchI GAGTC 5 cut(s) 1525, 2459, 2621, 2763, 3185
ScrFI CCNGG 8 cut(s) 266, 815, 1467, 1755, 2306, 2665, 2855, 3112
SduI GDGCHC 3 cut(s) 550, 737, 2590
SfcI CTRYAG 7 cut(s) 618, 897, 1659, 1746, 1860, 2778, 2964
Sfr274I CTCGAG 2 cut(s) 1344, 2907
SinI GGWCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
SlaI CTCGAG 2 cut(s) 1344, 2907
SmlI CTYRAG 4 cut(s) 1344, 2019, 2043, 2907
SmoI CTYRAG 4 cut(s) 1344, 2019, 2043, 2907
SnaBI TACGTA 1 cut(s) 1789
SseBI AGGCCT 1 cut(s) 1205
SsiI CCGC 4 cut(s) 198, 1577, 1583, 2760
SspI AATATT 1 cut(s) 2004
SspMI CTAG 8 cut(s) 659, 1523, 1907, 2213, 2325, 2399, 2913, 3098
SstI GAGCTC 1 cut(s) 550
StuI AGGCCT 1 cut(s) 1205
StyD4I CCNGG 8 cut(s) 264, 813, 1465, 1753, 2304, 2663, 2853, 3110
StyI CCWWGG 7 cut(s) 115, 595, 944, 2635, 2704, 2767, 2936
TaaI ACNGT 8 cut(s) 100, 799, 958, 2095, 2920, 2950, 3013, 3140
TaiI ACGT 4 cut(s) 1417, 1791, 2031, 2175
TaqI TCGA 8 cut(s) 166, 186, 1005, 1345, 2226, 2463, 2908, 3193
TatI WGTACW 6 cut(s) 141, 971, 1097, 1320, 2122, 2960
TauI GCSGC 1 cut(s) 1586
TfiI GAWTC 6 cut(s) 443, 572, 920, 2403, 2483, 2976
Tru1I TTAA 8 cut(s) 456, 1052, 1158, 1427, 1605, 1962, 3065, 3150
Tru9I TTAA 8 cut(s) 456, 1052, 1158, 1427, 1605, 1962, 3065, 3150
TseFI GTSAC 5 cut(s) 1178, 1918, 2089, 2762, 3170
Tsp45I GTSAC 5 cut(s) 1178, 1918, 2089, 2762, 3170
TspGWI ACGGA 1 cut(s) 397
Van91I CCANNNNNTGG 2 cut(s) 489, 2529
VpaK11BI GGWCC 6 cut(s) 100, 473, 911, 1418, 2513, 2903
VspI ATTAAT 1 cut(s) 3065
XbaI TCTAGA 1 cut(s) 1522
XceI RCATGY 4 cut(s) 14, 1186, 1538, 2135
XhoI CTCGAG 2 cut(s) 1344, 2907
XmiI GTMKAC 4 cut(s) 186, 1549, 2209, 3121
XmnI GAANNNNTTC 2 cut(s) 1043, 3035
XspI CTAG 8 cut(s) 659, 1523, 1907, 2213, 2325, 2399, 2913, 3098
ZraI GACGTC 1 cut(s) 2029
ZrmI AGTACT 3 cut(s) 973, 1099, 2962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.