Rh1AG055300

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000257
Physical Location & Seq
Reverse (-)
9030226 .. 9035135
4910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG055300.1

Sequence Viewer

Length: 2376 bp
ATGGCTAAGCATGTAGCTATCCTATTTTCTTATGCTTTCCCCACTCTTATACTAACCTTCAACTTCTTCCTGTGCCAAGCCATTGATGAAAATAGAACGGTCCACATTGTGTACCTTGGTTCACTTCCTGATAATGAGGTGTACTCACCAATGTCTCACCACCTTGGTTTACTCCAAAAAGTTGTCGACAGCAACTCCGCAGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCAAAGCTCACTGACCGGGAAAGGGAAAGGCTTGCTAACATGAAGGAAGTAGTCTCTGTATTTCCAAGCAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCCATGAGAAAATAAAACGGAATGTCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGACACTGGAATTTGGCCTGAATCGGAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGAAGGTGGCCAAAATTTCACTTGCAACAAGAAGCTAATTGGAGCTCGGTTTTACAATTCATCTTCAGAATCTGCAAGGGATGAACAAGGCCATGGAACCCACACAGCCTCAACTGCAGCAGGGAACGCTATAAAGGATGTGAGCTTTTATGGACTAGCACAAGGTACTGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTATGTGGTGCTCTAGGGTGCCCTACTGATGCTATATTGGCTGCCTTTGATGATGCTATTGCCGATGGAGTTGACATCATTACAGTTTCATTAGGAAACCCGGTTGCATCTTTATTACAGCATGACCCTATTGCTATCGGGGCTTTCCATGCAATGGCAAAGGGGATACTTACATCAAATTCTGCAGGCAACGGTGGTCCTGTTGATTCAAGTGTTTCAAGTGTAGCACCATGGATACTTACAATTGCAGCGAGTAGTACTGATCGTAGGATCATTGATAAGGTTGTTCTCGAAAATGGAACGACAATGGTTGGGGCTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTAATTGCTCCGAGTTCGAAGCTGGGAGATGTTCATACGGCTGCTTAGACAGTGGTTTAGTTAAGGGAAAGGTTGTGTTATGTGACGTGCCTAACAGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTCATTTTGAGAGTTGATTATGATGATGTTTCTGAAATTGTACCCTTTCCTACAACAGCTCTTGCCAACAAAGAGCATAGTCTGATAAATGCCGCTGGTGAATTTGCTTATGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACGTAAAGTTGCTATGCTCGGTCTTGGATCAGGCTGATGTTAGACTTATATCAGGAGATAACAGCACTTGCTCTACAGGCTCTGACAAAGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCCGTTGTCACACCAAACAAATCTTTTTCGTTTAAATTTCACAGAAAAGTTAAGAATGTTGGCCTTGCAAACTCCACTTACAAGGCCACAATATTCGCCAACTCTACTCAAGTTGACGTCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGGCTTTTGATGTGACAGTTGCCGGAAAAGGTGGCAAAAATTTTACTTGTAATAATAAGCTCATTGGTGCTCGGTTTTACAACTCATCCTCTCAGTCTGCAAGGGATGAAACTGGCCATGGAACACACACTGCCTCAACAGCAGCCGGGAATGCTGTAAAGGATGTCAGCTTTTATGGTATAGCCCAAGGCATTGCAAGAGGGGGAGTTCCCTCAGCAAGAGTTGCTGCGTATAGAGTCTGCAGTGACCAAGGATGCCCTACAGAGGCTATCTTGGCTGCTTTTGACGATGCTATTGCCGATGGAGTTGATATCATTACAATTTCAATAGGAAGCCCGGATGTAACTGTACTACTGCAGCATGATCCAATTGCTGTCGGATCTTTCCATGCAATGGCAAAGGGGATACTTACATCCAACTCGGCAGGCAATAATGGTCCTAGAGCTAGTACCGTAACAAGTGTAGCACCATGGTTACTTACAGTTGCAGCAGGTACTACAGATCGTAGAATGATTGACAAGGTTGTTCTTGGAAATGGTACAACTGTAGTTGGGGCTTCAGTGAACTCTTTCCAAATGACTGGGACAAGTTTTCCATTAATATATGGAAAAGATGCTACAACTCAATGCCTAGAGTTGATGCTCGGAAGTGTATACAAGACTAAGACCGTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

791

Amino Acids

83.71

Weight (kDa)

6.66

Isoelectric Point (pI)

26.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 110 2.2e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 467 5.3e-21 Subtilase family
fn3_6 PF17766 504 - 578 1.5e-15 Fibronectin type-III domain
Peptidase_S8 PF00082 596 - 735 5.3e-18 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1653
Acc36I ACCTGC 1 cut(s) 2180
AccB1I GGYRCC 2 cut(s) 732, 1660
AccB7I CCANNNNNTGG 3 cut(s) 489, 868, 2092
AccI GTMKAC 2 cut(s) 186, 2352
AciI CCGC 2 cut(s) 198, 1320
AclWI GGATC 7 cut(s) 992, 1348, 1443, 1507, 1518, 2057, 2086
AcoI YGGCCR 2 cut(s) 511, 1813
AcsI RAATTY 8 cut(s) 205, 321, 432, 517, 892, 1328, 1568, 1738
AcuI CTGAAG 3 cut(s) 552, 1017, 2241
AcyI GRCGYC 1 cut(s) 1650
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 9 cut(s) 113, 143, 670, 973, 1269, 2049, 2149, 2194, 2239
AfiI CCNNNNNNNGG 5 cut(s) 271, 489, 868, 1963, 2092
AgsI TTSAA 8 cut(s) 61, 924, 933, 1048, 1095, 1681, 1690, 2025
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 1 cut(s) 1375
Alw21I GWGCWC 3 cut(s) 550, 727, 1771
Alw26I GTCTC 2 cut(s) 159, 307
AlwI GGATC 7 cut(s) 992, 1348, 1443, 1507, 1518, 2057, 2086
AlwNI CAGNNNCTG 2 cut(s) 575, 1490
AoxI GGCC 7 cut(s) 437, 511, 592, 1203, 1594, 1617, 1813
ApoI RAATTY 8 cut(s) 205, 321, 432, 517, 892, 1328, 1568, 1738
AseI ATTAAT 1 cut(s) 2297
Asp700I GAANNNNTTC 2 cut(s) 1043, 2267
AspS9I GGNCC 4 cut(s) 100, 473, 911, 2135
AsuC2I CCSGG 4 cut(s) 266, 815, 1846, 2036
AsuHPI GGTGA 3 cut(s) 138, 149, 1337
AsuII TTCGAA 1 cut(s) 1113
AvaII GGWCC 4 cut(s) 100, 473, 911, 2135
BaeGI GKGCMC 1 cut(s) 737
BaeI ACNNNNGTAYC 2 cut(s) 2229, 2262
BalI TGGCCA 2 cut(s) 513, 1815
BanI GGYRCC 2 cut(s) 732, 1660
BanII GRGCYC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 1410
Bbv12I GWGCWC 3 cut(s) 550, 727, 1771
BbvCI CCTCAGC 2 cut(s) 691, 1912
BccI CCATC 4 cut(s) 336, 458, 773, 1994
BceAI ACGGC 2 cut(s) 1150, 1520
BcgI CGANNNNNNTGC 2 cut(s) 1976, 2010
BciT130I CCWGG 1 cut(s) 1377
BciVI GTATCC 3 cut(s) 873, 942, 2097
BcnI CCSGG 4 cut(s) 266, 815, 1846, 2036
BcoDI GTCTC 2 cut(s) 159, 307
BfaI CTAG 6 cut(s) 659, 728, 1529, 2139, 2145, 2330
BfmI CTRYAG 9 cut(s) 618, 897, 1368, 1482, 1939, 1959, 2054, 2196, 2244
BfuAI ACCTGC 1 cut(s) 2180
BfuI GTATCC 3 cut(s) 873, 942, 2097
BglII AGATCT 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 5 cut(s) 266, 815, 1377, 1846, 2036
Bme18I GGWCC 4 cut(s) 100, 473, 911, 2135
BmgBI CACGTC 1 cut(s) 1183
BmgT120I GGNCC 4 cut(s) 100, 473, 911, 2135
BmiI GGNNCC 4 cut(s) 601, 687, 734, 1662
BmrFI CCNGG 5 cut(s) 266, 815, 1377, 1846, 2036
BmrI ACTGGG 1 cut(s) 2289
BmsI GCATC 8 cut(s) 733, 757, 830, 1078, 1943, 1978, 2302, 2328
BmuI ACTGGG 1 cut(s) 2289
BpiI GAAGAC 1 cut(s) 1410
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 1 cut(s) 1236
Bpu10I CCTNAGC 2 cut(s) 691, 1912
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 1113
BpuEI CTTGAG 2 cut(s) 1626, 1650
BpuMI CCSGG 4 cut(s) 266, 815, 1846, 2036
BsaAI YACGTR 1 cut(s) 1411
BsaHI GRCGYC 1 cut(s) 1650
BsaXI ACNNNNNCTCC 4 cut(s) 179, 209, 223, 253
Bsc4I CCNNNNNNNGG 5 cut(s) 271, 489, 868, 1963, 2092
Bse1I ACTGG 3 cut(s) 433, 1816, 2284
Bse3DI GCAATG 3 cut(s) 873, 1890, 2097
BseBI CCWGG 1 cut(s) 1377
BseGI GGATG 9 cut(s) 328, 589, 646, 1784, 1810, 1867, 1958, 2044, 2111
BseLI CCNNNNNNNGG 5 cut(s) 271, 489, 868, 1963, 2092
BseMI GCAATG 3 cut(s) 873, 1890, 2097
BseMII CTCAG 4 cut(s) 347, 705, 1805, 1926
BseNI ACTGG 3 cut(s) 433, 1816, 2284
BseRI GAGGAG 3 cut(s) 468, 693, 1709
BseSI GKGCMC 1 cut(s) 737
BseYI CCCAGC 1 cut(s) 1118
BshFI GGCC 7 cut(s) 439, 513, 594, 1205, 1596, 1619, 1815
BshNI GGYRCC 2 cut(s) 732, 1660
BsiHKAI GWGCWC 3 cut(s) 550, 727, 1771
BsiSI CCGG 5 cut(s) 265, 815, 1722, 1845, 2036
BslFI GGGAC 3 cut(s) 365, 1072, 2296
BslI CCNNNNNNNGG 5 cut(s) 271, 489, 868, 1963, 2092
BsmAI GTCTC 2 cut(s) 159, 307
BsmFI GGGAC 3 cut(s) 365, 1072, 2296
BsmI GAATGC 1 cut(s) 1855
BsnI GGCC 7 cut(s) 439, 513, 594, 1205, 1596, 1619, 1815
Bsp119I TTCGAA 1 cut(s) 1113
Bsp1286I GDGCHC 4 cut(s) 550, 727, 737, 1771
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 4 cut(s) 595, 944, 1816, 2168
BspACI CCGC 2 cut(s) 198, 1320
BspANI GGCC 7 cut(s) 439, 513, 594, 1205, 1596, 1619, 1815
BspCNI CTCAG 4 cut(s) 346, 704, 1804, 1925
BspLI GGNNCC 4 cut(s) 601, 687, 734, 1662
BspMAI CTGCAG 4 cut(s) 622, 901, 1943, 2058
BspMI ACCTGC 1 cut(s) 2180
BspPI GGATC 7 cut(s) 992, 1348, 1443, 1507, 1518, 2057, 2086
BspT104I TTCGAA 1 cut(s) 1113
BspT107I GGYRCC 2 cut(s) 732, 1660
BsrDI GCAATG 3 cut(s) 873, 1890, 2097
BsrI ACTGG 3 cut(s) 433, 1816, 2284
BssNAI GTATAC 1 cut(s) 2353
BssNI GRCGYC 1 cut(s) 1650
BssT1I CCWWGG 8 cut(s) 115, 163, 595, 944, 1816, 1885, 1948, 2168
Bst1107I GTATAC 1 cut(s) 2353
Bst2UI CCWGG 1 cut(s) 1377
BstACI GRCGYC 1 cut(s) 1650
BstAPI GCANNNNNTGC 1 cut(s) 1922
BstBAI YACGTR 1 cut(s) 1411
BstBI TTCGAA 1 cut(s) 1113
BstC8I GCNNGC 3 cut(s) 282, 901, 2125
BstDEI CTNAG 8 cut(s) 6, 333, 691, 1141, 1398, 1791, 1912, 2361
BstDSI CCRYGG 5 cut(s) 595, 944, 1816, 2168, 2367
BstF5I GGATG 9 cut(s) 328, 589, 646, 1784, 1810, 1867, 1958, 2044, 2111
BstMAI GTCTC 2 cut(s) 159, 307
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 1 cut(s) 14
BstSCI CCNGG 5 cut(s) 264, 813, 1375, 1844, 2034
BstSFI CTRYAG 9 cut(s) 618, 897, 1368, 1482, 1939, 1959, 2054, 2196, 2244
BstSLI GKGCMC 1 cut(s) 737
BstSNI TACGTA 1 cut(s) 1411
BstV2I GAAGAC 1 cut(s) 1410
BstX2I RGATCY 4 cut(s) 344, 1340, 1499, 2078
BstXI CCANNNNNNTGG 1 cut(s) 2279
BstYI RGATCY 4 cut(s) 344, 1340, 1499, 2078
BstZ17I GTATAC 1 cut(s) 2353
BsuI GTATCC 3 cut(s) 873, 942, 2097
BsuRI GGCC 7 cut(s) 439, 513, 594, 1205, 1596, 1619, 1815
BtgI CCRYGG 5 cut(s) 595, 944, 1816, 2168, 2367
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 9 cut(s) 328, 589, 646, 1784, 1810, 1867, 1958, 2044, 2111
BtsI GCAGTG 2 cut(s) 1827, 1948
BtsIMutI CAGTG 9 cut(s) 244, 258, 397, 426, 1041, 1153, 1827, 1948, 2265
BveI ACCTGC 1 cut(s) 2180
Cac8I GCNNGC 3 cut(s) 282, 901, 2125
CaiI CAGNNNCTG 2 cut(s) 575, 1490
Cfr13I GGNCC 4 cut(s) 100, 473, 911, 2135
Csp6I GTAC 9 cut(s) 112, 142, 669, 972, 1268, 2048, 2148, 2193, 2238
CviQI GTAC 9 cut(s) 112, 142, 669, 972, 1268, 2048, 2148, 2193, 2238
DdeI CTNAG 8 cut(s) 6, 333, 691, 1141, 1398, 1791, 1912, 2361
DraI TTTAAA 2 cut(s) 457, 1567
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 511, 1813
Ecl136II GAGCTC 1 cut(s) 548
Eco105I TACGTA 1 cut(s) 1411
Eco130I CCWWGG 8 cut(s) 115, 163, 595, 944, 1816, 1885, 1948, 2168
Eco147I AGGCCT 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 550
Eco32I GATATC 1 cut(s) 2011
Eco47I GGWCC 4 cut(s) 100, 473, 911, 2135
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 3 cut(s) 552, 1017, 2241
EcoICRI GAGCTC 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 1375
EcoRV GATATC 1 cut(s) 2011
EcoT14I CCWWGG 8 cut(s) 115, 163, 595, 944, 1816, 1885, 1948, 2168
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 8 cut(s) 115, 163, 595, 944, 1816, 1885, 1948, 2168
FaqI GGGAC 3 cut(s) 365, 1072, 2296
FblI GTMKAC 2 cut(s) 186, 2352
FokI GGATG 9 cut(s) 315, 596, 653, 1771, 1817, 1874, 1965, 2051, 2098
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 6 cut(s) 659, 728, 1529, 2139, 2145, 2330
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 1 cut(s) 1236
HaeIII GGCC 7 cut(s) 439, 513, 594, 1205, 1596, 1619, 1815
HapII CCGG 5 cut(s) 265, 815, 1722, 1845, 2036
Hin1I GRCGYC 1 cut(s) 1650
HincII GTYRAC 3 cut(s) 187, 787, 1648
HindII GTYRAC 3 cut(s) 187, 787, 1648
HindIII AAGCTT 1 cut(s) 1392
HinfI GANTC 4 cut(s) 443, 572, 920, 1935
HpaII CCGG 5 cut(s) 265, 815, 1722, 1845, 2036
HphI GGTGA 3 cut(s) 138, 149, 1337
Hpy188I TCNGA 9 cut(s) 336, 448, 571, 1108, 1261, 1311, 1492, 2078, 2345
Hpy188III TCNNGA 6 cut(s) 128, 211, 1004, 1215, 1344, 1461
HpyAV CCTTC 6 cut(s) 67, 286, 500, 1533, 1687, 1693
HpyCH4IV ACGT 3 cut(s) 1182, 1410, 1650
HpyF3I CTNAG 8 cut(s) 6, 333, 691, 1141, 1398, 1791, 1912, 2361
HpySE526I ACGT 3 cut(s) 1182, 1410, 1650
Hsp92I GRCGYC 1 cut(s) 1650
LmnI GCTCC 3 cut(s) 545, 1109, 1217
LweI GCATC 8 cut(s) 733, 757, 830, 1078, 1943, 1978, 2302, 2328
MaeI CTAG 6 cut(s) 659, 728, 1529, 2139, 2145, 2330
MaeII ACGT 3 cut(s) 1182, 1410, 1650
MaeIII GTNAC 8 cut(s) 221, 1178, 1540, 1711, 1943, 2041, 2152, 2172
MboII GAAGA 3 cut(s) 58, 558, 1415
MfeI CAATTG 2 cut(s) 957, 2067
MflI RGATCY 4 cut(s) 344, 1340, 1499, 2078
MhlI GDGCHC 4 cut(s) 550, 727, 737, 1771
MlsI TGGCCA 2 cut(s) 513, 1815
MluNI TGGCCA 2 cut(s) 513, 1815
MlyI GAGTC 1 cut(s) 1944
MmeI TCCRAC 2 cut(s) 2056, 2139
Mox20I TGGCCA 2 cut(s) 513, 1815
MroXI GAANNNNTTC 2 cut(s) 1043, 2267
MscI TGGCCA 2 cut(s) 513, 1815
MseI TTAA 6 cut(s) 456, 1052, 1158, 1566, 1584, 2297
Msp20I TGGCCA 2 cut(s) 513, 1815
MspA1I CMGCKG 1 cut(s) 1322
MspI CCGG 5 cut(s) 265, 815, 1722, 1845, 2036
MspR9I CCNGG 5 cut(s) 266, 815, 1377, 1846, 2036
MunI CAATTG 2 cut(s) 957, 2067
Mva1269I GAATGC 1 cut(s) 1855
MvaI CCWGG 1 cut(s) 1377
NciI CCSGG 4 cut(s) 266, 815, 1846, 2036
NcoI CCATGG 4 cut(s) 595, 944, 1816, 2168
NlaIV GGNNCC 4 cut(s) 601, 687, 734, 1662
NmeAIII GCCGAG 1 cut(s) 2099
NmuCI GTSAC 4 cut(s) 1178, 1540, 1711, 1943
NspI RCATGY 1 cut(s) 14
NspV TTCGAA 1 cut(s) 1113
PasI CCCWGGG 1 cut(s) 1376
PceI AGGCCT 1 cut(s) 1205
PctI GAATGC 1 cut(s) 1855
PdmI GAANNNNTTC 2 cut(s) 1043, 2267
PfeI GAWTC 3 cut(s) 443, 572, 920
PflMI CCANNNNNTGG 3 cut(s) 489, 868, 2092
PleI GAGTC 1 cut(s) 1943
PpsI GAGTC 1 cut(s) 1943
Ppu21I YACGTR 1 cut(s) 1411
PshBI ATTAAT 1 cut(s) 2297
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 1375
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 4 cut(s) 601, 687, 734, 1662
PspPI GGNCC 4 cut(s) 100, 473, 911, 2135
PstI CTGCAG 4 cut(s) 622, 901, 1943, 2058
PstNI CAGNNNCTG 2 cut(s) 575, 1490
PsuI RGATCY 4 cut(s) 344, 1340, 1499, 2078
RsaI GTAC 9 cut(s) 113, 143, 670, 973, 1269, 2049, 2149, 2194, 2239
RsaNI GTAC 9 cut(s) 112, 142, 669, 972, 1268, 2048, 2148, 2193, 2238
SacI GAGCTC 1 cut(s) 550
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 6 cut(s) 456, 1052, 1158, 1566, 1584, 2297
Sau96I GGNCC 4 cut(s) 100, 473, 911, 2135
ScaI AGTACT 1 cut(s) 973
SchI GAGTC 1 cut(s) 1944
ScrFI CCNGG 5 cut(s) 266, 815, 1377, 1846, 2036
SduI GDGCHC 4 cut(s) 550, 727, 737, 1771
SfaNI GCATC 8 cut(s) 733, 757, 830, 1078, 1943, 1978, 2302, 2328
SfcI CTRYAG 9 cut(s) 618, 897, 1368, 1482, 1939, 1959, 2054, 2196, 2244
SfuI TTCGAA 1 cut(s) 1113
SinI GGWCC 4 cut(s) 100, 473, 911, 2135
SmlI CTYRAG 2 cut(s) 1641, 1665
SmoI CTYRAG 2 cut(s) 1641, 1665
SnaBI TACGTA 1 cut(s) 1411
SseBI AGGCCT 1 cut(s) 1205
SsiI CCGC 2 cut(s) 198, 1320
SspI AATATT 1 cut(s) 1626
SspMI CTAG 6 cut(s) 659, 728, 1529, 2139, 2145, 2330
SstI GAGCTC 1 cut(s) 550
StuI AGGCCT 1 cut(s) 1205
StyD4I CCNGG 5 cut(s) 264, 813, 1375, 1844, 2034
StyI CCWWGG 8 cut(s) 115, 163, 595, 944, 1816, 1885, 1948, 2168
TaiI ACGT 3 cut(s) 1185, 1413, 1653
TaqI TCGA 3 cut(s) 186, 1005, 1113
TaqII GACCGA 1 cut(s) 1417
TatI WGTACW 3 cut(s) 141, 971, 2047
TauI GCSGC 1 cut(s) 1322
TfiI GAWTC 3 cut(s) 443, 572, 920
Tru1I TTAA 6 cut(s) 456, 1052, 1158, 1566, 1584, 2297
Tru9I TTAA 6 cut(s) 456, 1052, 1158, 1566, 1584, 2297
TscAI CASTG 9 cut(s) 244, 265, 397, 433, 1041, 1153, 1834, 1948, 2265
TseFI GTSAC 4 cut(s) 1178, 1540, 1711, 1943
Tsp45I GTSAC 4 cut(s) 1178, 1540, 1711, 1943
TspDTI ATGAA 9 cut(s) 102, 305, 552, 600, 792, 1119, 1219, 1404, 1821
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 9 cut(s) 244, 265, 397, 433, 1041, 1153, 1834, 1948, 2265
Van91I CCANNNNNTGG 3 cut(s) 489, 868, 2092
VpaK11BI GGWCC 4 cut(s) 100, 473, 911, 2135
VspI ATTAAT 1 cut(s) 2297
XapI RAATTY 8 cut(s) 205, 321, 432, 517, 892, 1328, 1568, 1738
XceI RCATGY 1 cut(s) 14
XmiI GTMKAC 2 cut(s) 186, 2352
XmnI GAANNNNTTC 2 cut(s) 1043, 2267
XspI CTAG 6 cut(s) 659, 728, 1529, 2139, 2145, 2330
ZraI GACGTC 1 cut(s) 1651
ZrmI AGTACT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.