RLG00000030328

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000257
Physical Location & Seq
Forward (+)
59698765 .. 59700972
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030328

Sequence Viewer

Length: 2208 bp
ATGGCTCAGCATGTAGCTATCCTATTTTCTTATGCTTTCCCCACTCTTATACTAACCTTCAACTTCTTCCTGTGCAAAGCCATTGATGAAAATAGAACGGTCCACATTGTGTACCTGGGTTCACTTCCTGATAATGAGGTGTACTCACCAATGTCTCGCCACCTTGGTTTACTCCAAAAAGTTGTCAACAGCAACTCCACAGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCAAAGCTCACTGACCGGGAAAGGGAAAGGCTTGCTAACATGAAGGAAGTAGTCTCTGTATTTCCAAGCAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCCATGAGAAAATAAAACGGAATGTCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGACACTGGAATTTGGCCTGAATCGAAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGAAGGTGGCCAAAATTTCACTTGCAACAAGAAGCTAATTGGAGCTCGGGTTTACAATTCATCTTCAGAATCTGCAAGGGATGAACAAGGCCATGGAACCCACACAGCCTCAATTGCAGCAGGGAACGGTATAAAGGATGTGAGCTTTTATGGACTAGCACAAGGTACTGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGTGTATAAAGTATGTGGTGCTCTTGGGTGCCCTACTGATGCTATATTGGCTGCTTTTGATGATGCTATTGCCGATGGAGTTGACATCATTACAGTTTCATTAGGAAACCCGGTTGCATCTTTATTACAGCATGACCCTATTGCTATCGGGGCTTTCCATGCAATGGCAAAGGGGATACTTACATCAAATTCTGCAGGCAACGGTGGTCCTGTTGATTCAAGTGTTTCAAGTGTAGCACCATGGATACTTACAGTTGCAGCAAGTAGTACTGATCGTAGGATCATTGACAAGGTTGTTCTCGAAAATGGAACGACGATGGTTGGGGCTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTAATTGCTCCGAGTTCGAAGCTGGGAGATGTTCATACGGCTGCTTAGACAGTGGTTTAGTTAAGGGAAAGGTTGTGTTATGTGACGTGCCTAACAGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTCATTTTGAGAGTTGATTATGATGATGTTTCTCAAATTGTACCCTTTCCTGCAACAGCTTTTGCCGGCAAAGAGCATAGTCTGATAAAGTTGTACACGAACTCCACGAAAGATCCTCGAGCAAACATACTAAGAAGTGAAGAAGAAAGGGATACTGACGCACCTATTGTTGCTTCCTTCTCTTCACGTGGACCAAATTTAATTCTACCAGAAATTATCAAGCCAGATATAAGCGCCCCAGGGGTTACTATTTTAGCTGCCTTTTCACCTATTGCTTCAGTTACAAAGAGTTTTCAAGACACAAGGCATGTCAAATATAGTATACTATCTGGAACCTCCATGTCTTGTCCCCACGCAGCTGGTGCAGCTGCTTATGTTAAAGCATTCCATCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATGTTACTGACAATAACAGTGCCGCTGATGAATTTGCTTATGGATCTGGCCATATCAATCCTGTCAAAGCTATAAACCCAGGGCTTGTGTATGAAGCTTCTAAGGAAGACTACATAAAGTTGCTATGCTCGGTCTTGGATCAGGCCGATGTTAGACTTATATCAGGAGATAATAGCACTTGCTCTACAGGCTCTGACAACGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCCGTTGTCACACCAAACAAATCTTTTTCGTTCAAATTTCACAGAAAAGTCAAGAATGTTGGCCTTGCAAACTCCACTTACAAGGCCACAATATTCGCTAACTCTACTCAAGTTGACGTCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTGATGTGACAGTTGCCGGAAAAGGTTTGCCAGATGAAGTACAATCACATGTGTCTGCATCTCTGGTGTGGTCTGATGGAATTCATAGCGTTAGAAGTCCAATTCTTATCCACAAAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

736

Amino Acids

78.7

Weight (kDa)

7.59

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 110 7.7e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 575 1.9e-45 Subtilase family
fn3_6 PF17766 630 - 731 5e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2031
AccB1I GGYRCC 2 cut(s) 732, 2038
AccB7I CCANNNNNTGG 2 cut(s) 489, 868
AccI GTMKAC 1 cut(s) 1549
AciI CCGC 1 cut(s) 1698
AclWI GGATC 6 cut(s) 992, 1334, 1726, 1821, 1885, 1896
AcoI YGGCCR 2 cut(s) 511, 1723
AcsI RAATTY 9 cut(s) 205, 321, 432, 517, 892, 1423, 1706, 1946, 2163
AcuI CTGAAG 3 cut(s) 552, 1017, 1488
AcvI CACGTG 1 cut(s) 1415
AcyI GRCGYC 1 cut(s) 2028
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 7 cut(s) 113, 143, 670, 973, 1269, 1322, 2124
AfiI CCNNNNNNNGG 3 cut(s) 271, 489, 868
AflIII ACRYGT 1 cut(s) 2131
AgsI TTSAA 9 cut(s) 61, 924, 933, 1048, 1095, 1523, 1945, 2059, 2068
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 3 cut(s) 114, 1465, 1753
Alw21I GWGCWC 2 cut(s) 550, 727
Alw26I GTCTC 3 cut(s) 159, 307, 1632
AlwI GGATC 6 cut(s) 992, 1334, 1726, 1821, 1885, 1896
AlwNI CAGNNNCTG 2 cut(s) 575, 1868
Ama87I CYCGRG 2 cut(s) 549, 1344
AoxI GGCC 8 cut(s) 437, 511, 592, 1203, 1723, 1818, 1972, 1995
ApoI RAATTY 9 cut(s) 205, 321, 432, 517, 892, 1423, 1706, 1946, 2163
Asp700I GAANNNNTTC 1 cut(s) 1043
AspLEI GCGC 1 cut(s) 1463
AspS9I GGNCC 4 cut(s) 100, 473, 911, 1418
AsuC2I CCSGG 2 cut(s) 266, 815
AsuHPI GGTGA 2 cut(s) 138, 1485
AsuII TTCGAA 1 cut(s) 1113
AvaI CYCGRG 2 cut(s) 549, 1344
AvaII GGWCC 4 cut(s) 100, 473, 911, 1418
BaeGI GKGCMC 1 cut(s) 737
BalI TGGCCA 2 cut(s) 513, 1725
BanI GGYRCC 2 cut(s) 732, 2038
BanII GRGCYC 1 cut(s) 550
BbrPI CACGTG 1 cut(s) 1415
BbsI GAAGAC 2 cut(s) 1788, 2084
Bbv12I GWGCWC 2 cut(s) 550, 727
BbvCI CCTCAGC 1 cut(s) 691
BccI CCATC 7 cut(s) 336, 458, 773, 1015, 1623, 1646, 2153
BceAI ACGGC 2 cut(s) 1150, 1898
BciT130I CCWGG 3 cut(s) 116, 1467, 1755
BciVI GTATCC 3 cut(s) 873, 942, 1372
BcnI CCSGG 2 cut(s) 266, 815
BcoDI GTCTC 3 cut(s) 159, 307, 1632
BfaI CTAG 2 cut(s) 659, 1907
BfmI CTRYAG 3 cut(s) 897, 1659, 1860
BfoI RGCGCY 1 cut(s) 1464
BfuI GTATCC 3 cut(s) 873, 942, 1372
BglII AGATCT 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 5 cut(s) 116, 266, 815, 1467, 1755
Bme18I GGWCC 4 cut(s) 100, 473, 911, 1418
BmeT110I CYCGRG 2 cut(s) 549, 1344
BmgBI CACGTC 1 cut(s) 1183
BmgT120I GGNCC 4 cut(s) 100, 473, 911, 1418
BmiI GGNNCC 5 cut(s) 601, 687, 734, 1561, 2040
BmrFI CCNGG 5 cut(s) 116, 266, 815, 1467, 1755
BmsI GCATC 5 cut(s) 733, 757, 830, 1078, 2150
BpiI GAAGAC 2 cut(s) 1788, 2084
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 2 cut(s) 1236, 1614
Bpu10I CCTNAGC 1 cut(s) 691
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 1113
BpuEI CTTGAG 2 cut(s) 2004, 2028
BpuMI CCSGG 2 cut(s) 266, 815
BsaAI YACGTR 1 cut(s) 1415
BsaHI GRCGYC 1 cut(s) 2028
BsaI GGTCTC 1 cut(s) 1632
BsaJI CCNNGG 8 cut(s) 115, 163, 595, 944, 1465, 1466, 1753, 1754
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 489, 868
Bse118I RCCGGY 1 cut(s) 1292
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 3 cut(s) 116, 1467, 1755
BseDI CCNNGG 8 cut(s) 115, 163, 595, 944, 1465, 1466, 1753, 1754
BseGI GGATG 4 cut(s) 328, 589, 646, 1615
BseLI CCNNNNNNNGG 3 cut(s) 271, 489, 868
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 3 cut(s) 20, 347, 705
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 468, 693, 2087
BseSI GKGCMC 1 cut(s) 737
BseYI CCCAGC 1 cut(s) 1118
BsgI GTGCAG 1 cut(s) 1611
BshFI GGCC 8 cut(s) 439, 513, 594, 1205, 1725, 1820, 1974, 1997
BshNI GGYRCC 2 cut(s) 732, 2038
BsiHKAI GWGCWC 2 cut(s) 550, 727
BsiHKCI CYCGRG 2 cut(s) 549, 1344
BsiSI CCGG 4 cut(s) 265, 815, 1293, 2100
BslFI GGGAC 3 cut(s) 365, 1072, 1560
BslI CCNNNNNNNGG 3 cut(s) 271, 489, 868
BsmAI GTCTC 3 cut(s) 159, 307, 1632
BsmFI GGGAC 3 cut(s) 365, 1072, 1560
BsmI GAATGC 1 cut(s) 1610
BsnI GGCC 8 cut(s) 439, 513, 594, 1205, 1725, 1820, 1974, 1997
Bso31I GGTCTC 1 cut(s) 1632
BsoBI CYCGRG 2 cut(s) 549, 1344
Bsp119I TTCGAA 1 cut(s) 1113
Bsp1286I GDGCHC 3 cut(s) 550, 727, 737
Bsp1407I TGTACA 1 cut(s) 1320
Bsp143I GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 595, 944
BspACI CCGC 1 cut(s) 1698
BspANI GGCC 8 cut(s) 439, 513, 594, 1205, 1725, 1820, 1974, 1997
BspCNI CTCAG 3 cut(s) 19, 346, 704
BspLI GGNNCC 5 cut(s) 601, 687, 734, 1561, 2040
BspMAI CTGCAG 1 cut(s) 901
BspPI GGATC 6 cut(s) 992, 1334, 1726, 1821, 1885, 1896
BspQI GCTCTTC 1 cut(s) 443
BspT104I TTCGAA 1 cut(s) 1113
BspT107I GGYRCC 2 cut(s) 732, 2038
BspTNI GGTCTC 1 cut(s) 1632
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 1292
BsrGI TGTACA 1 cut(s) 1320
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 1292
BssECI CCNNGG 8 cut(s) 115, 163, 595, 944, 1465, 1466, 1753, 1754
BssMI GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
BssNAI GTATAC 1 cut(s) 1550
BssNI GRCGYC 1 cut(s) 2028
BssT1I CCWWGG 3 cut(s) 163, 595, 944
Bst1107I GTATAC 1 cut(s) 1550
Bst2UI CCWGG 3 cut(s) 116, 1467, 1755
Bst4CI ACNGT 8 cut(s) 100, 632, 799, 908, 958, 1148, 1694, 2095
Bst6I CTCTTC 2 cut(s) 443, 1414
BstACI GRCGYC 1 cut(s) 2028
BstAPI GCANNNNNTGC 1 cut(s) 1589
BstAUI TGTACA 1 cut(s) 1320
BstBAI YACGTR 1 cut(s) 1415
BstBI TTCGAA 1 cut(s) 1113
BstC8I GCNNGC 3 cut(s) 282, 901, 1294
BstDEI CTNAG 6 cut(s) 6, 333, 691, 1141, 1358, 1776
BstDSI CCRYGG 2 cut(s) 595, 944
BstF5I GGATG 4 cut(s) 328, 589, 646, 1615
BstH2I RGCGCY 1 cut(s) 1464
BstHHI GCGC 1 cut(s) 1463
BstKTI GATC 9 cut(s) 347, 979, 987, 1077, 1342, 1721, 1816, 1880, 1891
BstMAI GTCTC 3 cut(s) 159, 307, 1632
BstMBI GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
BstMWI GCNNNNNNNGC 8 cut(s) 617, 701, 752, 854, 863, 1589, 1592, 1863
BstNI CCWGG 3 cut(s) 116, 1467, 1755
BstNSI RCATGY 3 cut(s) 14, 1538, 2135
BstSCI CCNGG 5 cut(s) 114, 264, 813, 1465, 1753
BstSFI CTRYAG 3 cut(s) 897, 1659, 1860
BstSLI GKGCMC 1 cut(s) 737
BstV2I GAAGAC 2 cut(s) 1788, 2084
BstX2I RGATCY 4 cut(s) 344, 1339, 1718, 1877
BstXI CCANNNNNNTGG 1 cut(s) 1586
BstYI RGATCY 4 cut(s) 344, 1339, 1718, 1877
BstZ17I GTATAC 1 cut(s) 1550
BsuI GTATCC 3 cut(s) 873, 942, 1372
BsuRI GGCC 8 cut(s) 439, 513, 594, 1205, 1725, 1820, 1974, 1997
BtgI CCRYGG 2 cut(s) 595, 944
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 4 cut(s) 328, 589, 646, 1615
BtsIMutI CAGTG 7 cut(s) 244, 258, 397, 426, 1041, 1153, 1699
Cac8I GCNNGC 3 cut(s) 282, 901, 1294
CaiI CAGNNNCTG 2 cut(s) 575, 1868
CfoI GCGC 1 cut(s) 1463
Cfr10I RCCGGY 1 cut(s) 1292
Cfr13I GGNCC 4 cut(s) 100, 473, 911, 1418
CseI GACGC 1 cut(s) 1394
Csp6I GTAC 7 cut(s) 112, 142, 669, 972, 1268, 1321, 2123
CviQI GTAC 7 cut(s) 112, 142, 669, 972, 1268, 1321, 2123
DdeI CTNAG 6 cut(s) 6, 333, 691, 1141, 1358, 1776
DpnI GATC 9 cut(s) 346, 978, 986, 1076, 1341, 1720, 1815, 1879, 1890
DpnII GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 511, 1723
Eam1104I CTCTTC 2 cut(s) 443, 1414
EarI CTCTTC 2 cut(s) 443, 1414
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 163, 595, 944
Eco147I AGGCCT 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 1632
Eco47I GGWCC 4 cut(s) 100, 473, 911, 1418
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 3 cut(s) 552, 1017, 1488
Eco72I CACGTG 1 cut(s) 1415
Eco88I CYCGRG 2 cut(s) 549, 1344
EcoICRI GAGCTC 1 cut(s) 548
EcoRI GAATTC 2 cut(s) 321, 2163
EcoRII CCWGG 3 cut(s) 114, 1465, 1753
EcoT14I CCWWGG 3 cut(s) 163, 595, 944
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 3 cut(s) 163, 595, 944
FaqI GGGAC 3 cut(s) 365, 1072, 1560
FblI GTMKAC 1 cut(s) 1549
FokI GGATG 4 cut(s) 315, 596, 653, 1602
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 2 cut(s) 659, 1907
GlaI GCGC 1 cut(s) 1462
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 2 cut(s) 1236, 1614
HaeII RGCGCY 1 cut(s) 1464
HaeIII GGCC 8 cut(s) 439, 513, 594, 1205, 1725, 1820, 1974, 1997
HapII CCGG 4 cut(s) 265, 815, 1293, 2100
HgaI GACGC 1 cut(s) 1394
HhaI GCGC 1 cut(s) 1463
Hin1I GRCGYC 1 cut(s) 2028
Hin6I GCGC 1 cut(s) 1461
HinP1I GCGC 1 cut(s) 1461
HincII GTYRAC 3 cut(s) 187, 787, 2026
HindII GTYRAC 3 cut(s) 187, 787, 2026
HindIII AAGCTT 1 cut(s) 1770
HinfI GANTC 3 cut(s) 443, 572, 920
HpaII CCGG 4 cut(s) 265, 815, 1293, 2100
HphI GGTGA 2 cut(s) 138, 1485
Hpy188I TCNGA 6 cut(s) 336, 571, 1108, 1311, 1870, 2158
Hpy188III TCNNGA 9 cut(s) 128, 211, 1004, 1215, 1523, 1557, 1619, 1839, 1963
Hpy99I CGWCG 1 cut(s) 1021
HpyAV CCTTC 6 cut(s) 67, 286, 500, 1414, 1911, 2065
HpyCH4III ACNGT 8 cut(s) 100, 632, 799, 908, 958, 1148, 1694, 2095
HpyCH4IV ACGT 3 cut(s) 1182, 1414, 2028
HpyF10VI GCNNNNNNNGC 8 cut(s) 617, 701, 752, 854, 863, 1589, 1592, 1863
HpyF3I CTNAG 6 cut(s) 6, 333, 691, 1141, 1358, 1776
HpySE526I ACGT 3 cut(s) 1182, 1414, 2028
Hsp92I GRCGYC 1 cut(s) 2028
HspAI GCGC 1 cut(s) 1461
KroI GCCGGC 1 cut(s) 1292
KroNI GCCGGC 1 cut(s) 1294
Kzo9I GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 3 cut(s) 545, 1109, 1217
LweI GCATC 5 cut(s) 733, 757, 830, 1078, 2150
MaeI CTAG 2 cut(s) 659, 1907
MaeII ACGT 3 cut(s) 1182, 1414, 2028
MaeIII GTNAC 7 cut(s) 221, 1178, 1471, 1507, 1678, 1918, 2089
MalI GATC 9 cut(s) 346, 978, 986, 1076, 1341, 1720, 1815, 1879, 1890
MboI GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
MboII GAAGA 8 cut(s) 58, 460, 558, 1379, 1382, 1401, 1793, 2089
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 4 cut(s) 344, 1339, 1718, 1877
MhlI GDGCHC 3 cut(s) 550, 727, 737
MlsI TGGCCA 2 cut(s) 513, 1725
MluNI TGGCCA 2 cut(s) 513, 1725
Mox20I TGGCCA 2 cut(s) 513, 1725
MroNI GCCGGC 1 cut(s) 1292
MroXI GAANNNNTTC 1 cut(s) 1043
MscI TGGCCA 2 cut(s) 513, 1725
MseI TTAA 5 cut(s) 456, 1052, 1158, 1427, 1605
Msp20I TGGCCA 2 cut(s) 513, 1725
MspA1I CMGCKG 3 cut(s) 1586, 1595, 1700
MspI CCGG 4 cut(s) 265, 815, 1293, 2100
MspR9I CCNGG 5 cut(s) 116, 266, 815, 1467, 1755
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 1610
MvaI CCWGG 3 cut(s) 116, 1467, 1755
MwoI GCNNNNNNNGC 8 cut(s) 617, 701, 752, 854, 863, 1589, 1592, 1863
NaeI GCCGGC 1 cut(s) 1294
NciI CCSGG 2 cut(s) 266, 815
NcoI CCATGG 2 cut(s) 595, 944
NdeII GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
NgoMIV GCCGGC 1 cut(s) 1292
NlaIV GGNNCC 5 cut(s) 601, 687, 734, 1561, 2040
NmuCI GTSAC 3 cut(s) 1178, 1918, 2089
NspI RCATGY 3 cut(s) 14, 1538, 2135
NspV TTCGAA 1 cut(s) 1113
PaeR7I CTCGAG 1 cut(s) 1344
PasI CCCWGGG 2 cut(s) 1466, 1754
PceI AGGCCT 1 cut(s) 1205
PciI ACATGT 1 cut(s) 2131
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 1331
PctI GAATGC 1 cut(s) 1610
PdiI GCCGGC 1 cut(s) 1294
PdmI GAANNNNTTC 1 cut(s) 1043
PfeI GAWTC 3 cut(s) 443, 572, 920
PflMI CCANNNNNTGG 2 cut(s) 489, 868
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
Ppu21I YACGTR 1 cut(s) 1415
PscI ACATGT 1 cut(s) 2131
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 3 cut(s) 114, 1465, 1753
PspCI CACGTG 1 cut(s) 1415
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 3 cut(s) 114, 1465, 1753
PspN4I GGNNCC 5 cut(s) 601, 687, 734, 1561, 2040
PspPI GGNCC 4 cut(s) 100, 473, 911, 1418
PspXI VCTCGAGB 1 cut(s) 1344
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 2 cut(s) 575, 1868
PsuI RGATCY 4 cut(s) 344, 1339, 1718, 1877
PvuII CAGCTG 2 cut(s) 1586, 1595
RsaI GTAC 7 cut(s) 113, 143, 670, 973, 1269, 1322, 2124
RsaNI GTAC 7 cut(s) 112, 142, 669, 972, 1268, 1321, 2123
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 5 cut(s) 456, 1052, 1158, 1427, 1605
Sau3AI GATC 9 cut(s) 344, 976, 984, 1074, 1339, 1718, 1813, 1877, 1888
Sau96I GGNCC 4 cut(s) 100, 473, 911, 1418
ScaI AGTACT 1 cut(s) 973
ScrFI CCNGG 5 cut(s) 116, 266, 815, 1467, 1755
SduI GDGCHC 3 cut(s) 550, 727, 737
SfaNI GCATC 5 cut(s) 733, 757, 830, 1078, 2150
SfcI CTRYAG 3 cut(s) 897, 1659, 1860
Sfr274I CTCGAG 1 cut(s) 1344
SfuI TTCGAA 1 cut(s) 1113
SinI GGWCC 4 cut(s) 100, 473, 911, 1418
SlaI CTCGAG 1 cut(s) 1344
SmlI CTYRAG 3 cut(s) 1344, 2019, 2043
SmoI CTYRAG 3 cut(s) 1344, 2019, 2043
SseBI AGGCCT 1 cut(s) 1205
SsiI CCGC 1 cut(s) 1698
SspI AATATT 1 cut(s) 2004
SspMI CTAG 2 cut(s) 659, 1907
SstI GAGCTC 1 cut(s) 550
StuI AGGCCT 1 cut(s) 1205
StyD4I CCNGG 5 cut(s) 114, 264, 813, 1465, 1753
StyI CCWWGG 3 cut(s) 163, 595, 944
TaaI ACNGT 8 cut(s) 100, 632, 799, 908, 958, 1148, 1694, 2095
TaiI ACGT 3 cut(s) 1185, 1417, 2031
TaqI TCGA 4 cut(s) 446, 1005, 1113, 1345
TaqII GACCGA 1 cut(s) 1795
TatI WGTACW 4 cut(s) 141, 971, 1320, 2122
TauI GCSGC 1 cut(s) 1700
TfiI GAWTC 3 cut(s) 443, 572, 920
Tru1I TTAA 5 cut(s) 456, 1052, 1158, 1427, 1605
Tru9I TTAA 5 cut(s) 456, 1052, 1158, 1427, 1605
TscAI CASTG 7 cut(s) 244, 265, 397, 433, 1041, 1153, 1699
TseFI GTSAC 3 cut(s) 1178, 1918, 2089
Tsp45I GTSAC 3 cut(s) 1178, 1918, 2089
TspGWI ACGGA 2 cut(s) 397, 1890
TspRI CASTG 7 cut(s) 244, 265, 397, 433, 1041, 1153, 1699
Van91I CCANNNNNTGG 2 cut(s) 489, 868
VpaK11BI GGWCC 4 cut(s) 100, 473, 911, 1418
XapI RAATTY 9 cut(s) 205, 321, 432, 517, 892, 1423, 1706, 1946, 2163
XceI RCATGY 3 cut(s) 14, 1538, 2135
XhoI CTCGAG 1 cut(s) 1344
XmiI GTMKAC 1 cut(s) 1549
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 2 cut(s) 659, 1907
ZraI GACGTC 1 cut(s) 2029
ZrmI AGTACT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.