RchiOBHm_Chr1g0323281

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000257
Physical Location & Seq
Reverse (-)
10827557 .. 10829758
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55319

Sequence Viewer

Length: 2202 bp
ATGGCTAAGCATGGAGCATTCCTATTTTTTTATGCTTTCTCTACTCTCATACTTACTATGAGCTTATTATGCAATGCCATTGATCATGAAGATAGAAAGGTTCATATAGTGTATCTGGGGTCACTTCCGGATGATGAGGAGTACTCGCCAAGGTCTCATCACCTCGATATACTACAAAGAGTTGTTGATGGCAGTTCTGCTGAAAATTGCTTGATAAGGAGCTTCAATGGATTTGCTGCAAAGCTCAGTGATCAAGAAAAAGAAAAGATTGCTAACATGGAGGAGGTGGTCTCCGTCTTTCCTAGCAGAACCTTCCAACTTCAAACGACGAGGTCATGGGACTTCATGGGTCTCAAGCTGTCAAGTCCTCGAAAAGCCACTGCTGAGAGTAATGTTATTGTTGCTGTGATTGACTCTGGAATTGACCCTGATTCGGAGAGCTTTAGAGATGAAGGTTTTGGTGCTGCTCCTATGAAGTGGAAAGGTGTTTGTGAAGGTGGCGAAAATTTCAGTTGCAACAACAAGATAATTGGAGCTCGTTTTTACGAACCAGCAGAGTCTGCAAGGGATGAAGACGGCCACGGAACCCACACTGCCTCAACGGCAGCAGGGAATGAGGTAAGGGATGTAAGCTTTTACGGATTAGAACCAGGTACAGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTCTCTGGGGCTACAGGGTGCTCTTCAGAGGCTATATTGGCTACTTTTGACGATGCAATTGCTGATGGAGTTGACATTGTGTCAGTCTCACTTGGAAGTGCAAGTCCCCTTCCTTTTGATAATGATCCTATAGCAATTGGTTCTTATCATGCAATGAAGAGAGGGATATTGACATCAAACTCTGCAGGCAACAGTGGTCCTGGAGCTGGTACTGTAACAAGTGTAGCGCCGTGGATGCTTGCAGTTGCAGCAAGTAGCAGAGATCGTCGGATCATTGACAAGGCCGTTCTTGGAAGTAGGACACTAATAGGGGCTTCAGTGAACTCTTTCATATTGAAAGGTAGTTTTCCACTGATATATGGAAAAGATGCTTCAAGTAACTGCCCCGAATACTATGCTAGGACTTGTGCAGATGGTTGCCTAGACAGTAATTCAGTTCGGAACAAGATCGTGCTATGTGACCGGCCCGCTGGATTTTATGAGGCTGATCGAGCTGGTGCACGAGGCGTAATTTTCTACAATCCCATACCTGATGATGCTTCACTTGTCCCAATGCCTGCTGTGGGTTTGAGATCCGAAGACTATAATGTGGCCATATCTTACATGAACTCGACAAAAGATCCTCAAGCAAGCATATCAAAGAGTGAAAACATAAAAGATCCAGCTGCACCTGAAGTTGTTTCTTTCTCTTCGCGCGGCCCTAATCAAATTTTACCTGAAATTATTAAGCCAGATGTAACTGGCCCGGGAGTGACCATTATAGCTGCATATCCGAGTGATGTTCCTGTCACAAGTGGCTCTAGAGACACAAGGCGGGTCAAGTACAATATACTATCCGGAACCTCCATGTCTTGCCCCCACGCTACTGGTGTAGCTGCATATGTAAAGGAGTTCCACCCTGATTGGTCTCCATCTGCCATTAAATCTGCTCTTATGACTACAGCTTGGCCTATGAATCATACAACCAACAGCTCATGCCCCGGTGAATTTGCATATGGATCCGGTCATATCAATCCTCTAAAAGCTATTGACCCAGGGCTTGTGTATGATGCTTCTGCAGAGGATTACATTAAGTTACTCTGCTTGGACATGGACGAGGCGAGAGTCAGACTTATATCAGGAGATAACAGCACTTGTCCTACAGCTTCTGAACAAGGATCGGCAAAGGATCTCAACTACCCTTCAATATCAGCTATTGTTACACCAATGACACGTTTTACGGTGACAATACGTAGAAGAGTTACAAATGTTGGCCTTGCAAATTCCACTTACAAGGCCGAAATCATGCCAAACTCTCAAGTTGAAATCAAAGTGGAGCCTCAAGTTCTTTCCTTCAAGTCCGTGAACGAGGAGAAGACTTTTGATTTGACCATTGTTGGAAGAGGGTTGCCAGATGGATCACATATGTCTTCGTCCTTGGTTTGGTCCGACGGAACTCATAAGGTTAAAAGTCCAGTTCTAGTGCAAAGCTTATCAGCAGCAGCATCATCACGGTTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

733

Amino Acids

78.42

Weight (kDa)

5.61

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 108 6.8e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 130 - 554 9.2e-45 Subtilase family
fn3_6 PF17766 623 - 721 1.1e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1393, 1395
AccIII TCCGGA 2 cut(s) 127, 1535
AciI CCGC 3 cut(s) 1167, 1395, 1513
AcoI YGGCCR 2 cut(s) 577, 1290
AcsI RAATTY 4 cut(s) 505, 1407, 1685, 1960
AcuI CTGAAG 3 cut(s) 708, 999, 1392
AfaI GTAC 4 cut(s) 143, 655, 910, 1523
AfiI CCNNNNNNNGG 4 cut(s) 433, 905, 1262, 2121
AflIII ACRYGT 1 cut(s) 1910
AgsI TTSAA 7 cut(s) 226, 323, 1036, 1074, 1884, 2003, 2035
AhdI GACNNNNNGTC 1 cut(s) 778
AjnI CCWGG 3 cut(s) 649, 898, 1732
Alw21I GWGCWC 3 cut(s) 538, 722, 1201
Alw26I GTCTC 7 cut(s) 159, 295, 356, 706, 790, 1497, 1611
Alw44I GTGCAC 1 cut(s) 1197
AlwNI CAGNNNCTG 2 cut(s) 560, 1847
Ama87I CYCGRG 1 cut(s) 1444
Aor13HI TCCGGA 2 cut(s) 127, 1535
AoxI GGCC 9 cut(s) 577, 981, 1163, 1290, 1396, 1441, 1646, 1951, 1974
ApaLI GTGCAC 1 cut(s) 1197
ApoI RAATTY 4 cut(s) 505, 1407, 1685, 1960
ArsI GACNNNNNNTTYG 2 cut(s) 2047, 2079
Asp700I GAANNNNTTC 1 cut(s) 1025
AspLEI GCGC 2 cut(s) 928, 1395
AspS9I GGNCC 5 cut(s) 896, 1164, 1397, 1442, 2124
AsuC2I CCSGG 3 cut(s) 1445, 1446, 1680
AsuHPI GGTGA 3 cut(s) 152, 1694, 1933
AvaI CYCGRG 1 cut(s) 1444
AvaII GGWCC 2 cut(s) 896, 2124
BaeGI GKGCMC 1 cut(s) 1201
BalI TGGCCA 1 cut(s) 1292
BamHI GGATCC 1 cut(s) 1697
BanII GRGCYC 1 cut(s) 538
BauI CACGAG 1 cut(s) 1200
BbsI GAAGAC 4 cut(s) 579, 1284, 2060, 2100
Bbv12I GWGCWC 3 cut(s) 538, 722, 1201
BbvCI CCTCAGC 1 cut(s) 676
BccI CCATC 5 cut(s) 182, 758, 1106, 1618, 2087
BceAI ACGGC 4 cut(s) 592, 618, 913, 968
BcgI CGANNNNNNTGC 4 cut(s) 740, 774, 1076, 1110
BciT130I CCWGG 3 cut(s) 651, 900, 1734
BclI TGATCA 2 cut(s) 82, 250
BcnI CCSGG 3 cut(s) 1445, 1446, 1680
BcoDI GTCTC 7 cut(s) 159, 295, 356, 706, 790, 1497, 1611
BfaI CTAG 5 cut(s) 303, 1098, 1121, 1500, 2159
BfmI CTRYAG 6 cut(s) 711, 828, 882, 1638, 1755, 1839
BfoI RGCGCY 1 cut(s) 929
BglI GCCNNNNNGGC 1 cut(s) 602
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 143
Bme1390I CCNGG 6 cut(s) 651, 900, 1445, 1446, 1680, 1734
Bme18I GGWCC 2 cut(s) 896, 2124
BmeRI GACNNNNNGTC 1 cut(s) 778
BmeT110I CYCGRG 1 cut(s) 1444
BmgT120I GGNCC 5 cut(s) 896, 1164, 1397, 1442, 2124
BmiI GGNNCC 5 cut(s) 586, 672, 1540, 1699, 2016
BmrFI CCNGG 6 cut(s) 651, 900, 1445, 1446, 1680, 1734
BmsI GCATC 6 cut(s) 742, 924, 1057, 1225, 1738, 2192
BpiI GAAGAC 4 cut(s) 579, 1284, 2060, 2100
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 275, 307
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 676
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 338, 1308, 1980, 2004
BpuMI CCSGG 3 cut(s) 1445, 1446, 1680
BsaAI YACGTR 1 cut(s) 1931
BsaBI GATNNNNATC 1 cut(s) 822
BsaI GGTCTC 4 cut(s) 159, 295, 356, 1611
BsaJI CCNNGG 8 cut(s) 149, 580, 929, 1444, 1678, 1732, 1733, 2115
BsaWI WCCGGW 3 cut(s) 127, 1535, 1700
Bsc4I CCNNNNNNNGG 4 cut(s) 433, 905, 1262, 2121
Bse118I RCCGGY 1 cut(s) 1161
Bse1I ACTGG 3 cut(s) 1444, 1570, 2153
Bse3DI GCAATG 2 cut(s) 79, 858
Bse8I GATNNNNATC 1 cut(s) 822
BseAI TCCGGA 2 cut(s) 127, 1535
BseBI CCWGG 3 cut(s) 651, 900, 1734
BseDI CCNNGG 8 cut(s) 149, 580, 929, 1444, 1678, 1732, 1733, 2115
BseGI GGATG 4 cut(s) 136, 574, 631, 939
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 4 cut(s) 433, 905, 1262, 2121
BseMI GCAATG 2 cut(s) 79, 858
BseMII CTCAG 3 cut(s) 259, 375, 690
BseNI ACTGG 3 cut(s) 1444, 1570, 2153
BseRI GAGGAG 4 cut(s) 152, 296, 678, 2063
BseSI GKGCMC 1 cut(s) 1201
BsgI GTGCAG 2 cut(s) 1128, 1350
Bsh1236I CGCG 2 cut(s) 1393, 1395
BshFI GGCC 9 cut(s) 579, 983, 1165, 1292, 1398, 1443, 1648, 1953, 1976
BsiHKAI GWGCWC 3 cut(s) 538, 722, 1201
BsiHKCI CYCGRG 1 cut(s) 1444
BsiSI CCGG 6 cut(s) 128, 1162, 1445, 1536, 1680, 1701
BslFI GGGAC 3 cut(s) 353, 789, 1232
BslI CCNNNNNNNGG 4 cut(s) 433, 905, 1262, 2121
BsmAI GTCTC 7 cut(s) 159, 295, 356, 706, 790, 1497, 1611
BsmFI GGGAC 3 cut(s) 353, 789, 1232
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 9 cut(s) 579, 983, 1165, 1292, 1398, 1443, 1648, 1953, 1976
Bso31I GGTCTC 4 cut(s) 159, 295, 356, 1611
BsoBI CYCGRG 1 cut(s) 1444
Bsp1286I GDGCHC 3 cut(s) 538, 722, 1201
Bsp13I TCCGGA 2 cut(s) 127, 1535
Bsp1720I GCTNAGC 1 cut(s) 6
BspACI CCGC 3 cut(s) 1167, 1395, 1513
BspANI GGCC 9 cut(s) 579, 983, 1165, 1292, 1398, 1443, 1648, 1953, 1976
BspCNI CTCAG 3 cut(s) 258, 376, 689
BspEI TCCGGA 2 cut(s) 127, 1535
BspFNI CGCG 2 cut(s) 1393, 1395
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 5 cut(s) 586, 672, 1540, 1699, 2016
BspMAI CTGCAG 2 cut(s) 886, 1759
BspQI GCTCTTC 1 cut(s) 727
BspTNI GGTCTC 4 cut(s) 159, 295, 356, 1611
BsrDI GCAATG 2 cut(s) 79, 858
BsrFI RCCGGY 1 cut(s) 1161
BsrI ACTGG 3 cut(s) 1444, 1570, 2153
BssAI RCCGGY 1 cut(s) 1161
BssECI CCNNGG 8 cut(s) 149, 580, 929, 1444, 1678, 1732, 1733, 2115
BssSI CACGAG 1 cut(s) 1200
BssT1I CCWWGG 2 cut(s) 149, 2115
Bst2BI CACGAG 1 cut(s) 1200
Bst2UI CCWGG 3 cut(s) 651, 900, 1734
Bst4CI ACNGT 5 cut(s) 893, 913, 1127, 1921, 2193
Bst6I CTCTTC 5 cut(s) 727, 851, 1393, 1930, 2074
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 1931
BstC8I GCNNGC 5 cut(s) 886, 939, 1167, 1257, 1330
BstDEI CTNAG 4 cut(s) 6, 245, 384, 676
BstDSI CCRYGG 2 cut(s) 580, 929
BstF5I GGATG 4 cut(s) 136, 574, 631, 939
BstFNI CGCG 2 cut(s) 1393, 1395
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 2 cut(s) 928, 1395
BstMAI GTCTC 7 cut(s) 159, 295, 356, 706, 790, 1497, 1611
BstMWI GCNNNNNNNGC 8 cut(s) 69, 560, 602, 686, 737, 934, 947, 1190
BstNI CCWGG 3 cut(s) 651, 900, 1734
BstSCI CCNGG 6 cut(s) 649, 898, 1443, 1444, 1678, 1732
BstSFI CTRYAG 6 cut(s) 711, 828, 882, 1638, 1755, 1839
BstSLI GKGCMC 1 cut(s) 1201
BstSNI TACGTA 1 cut(s) 1931
BstUI CGCG 2 cut(s) 1393, 1395
BstV2I GAAGAC 4 cut(s) 579, 1284, 2060, 2100
BstX2I RGATCY 5 cut(s) 1271, 1318, 1357, 1697, 1867
BstXI CCANNNNNNTGG 1 cut(s) 1565
BstYI RGATCY 5 cut(s) 1271, 1318, 1357, 1697, 1867
BsuRI GGCC 9 cut(s) 579, 983, 1165, 1292, 1398, 1443, 1648, 1953, 1976
BtgI CCRYGG 2 cut(s) 580, 929
BtsCI GGATG 4 cut(s) 136, 574, 631, 939
BtsI GCAGTG 2 cut(s) 378, 591
BtsIMutI CAGTG 6 cut(s) 253, 378, 591, 898, 1023, 1049
Cac8I GCNNGC 5 cut(s) 886, 939, 1167, 1257, 1330
CaiI CAGNNNCTG 2 cut(s) 560, 1847
CciI TCATGA 1 cut(s) 85
CfoI GCGC 2 cut(s) 928, 1395
Cfr10I RCCGGY 1 cut(s) 1161
Cfr13I GGNCC 5 cut(s) 896, 1164, 1397, 1442, 2124
Cfr9I CCCGGG 1 cut(s) 1444
CsiI ACCWGGT 1 cut(s) 649
Csp6I GTAC 4 cut(s) 142, 654, 909, 1522
CspCI CAANNNNNGTGG 2 cut(s) 1583, 1618
CviQI GTAC 4 cut(s) 142, 654, 909, 1522
DdeI CTNAG 4 cut(s) 6, 245, 384, 676
DriI GACNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 2 cut(s) 577, 1290
Eam1104I CTCTTC 5 cut(s) 727, 851, 1393, 1930, 2074
Eam1105I GACNNNNNGTC 1 cut(s) 778
EarI CTCTTC 5 cut(s) 727, 851, 1393, 1930, 2074
Ecl136II GAGCTC 1 cut(s) 536
Eco105I TACGTA 1 cut(s) 1931
Eco130I CCWWGG 2 cut(s) 149, 2115
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 4 cut(s) 159, 295, 356, 1611
Eco47I GGWCC 2 cut(s) 896, 2124
Eco53kI GAGCTC 1 cut(s) 536
Eco57I CTGAAG 3 cut(s) 708, 999, 1392
Eco88I CYCGRG 1 cut(s) 1444
EcoICRI GAGCTC 1 cut(s) 536
EcoRII CCWGG 3 cut(s) 649, 898, 1732
EcoT14I CCWWGG 2 cut(s) 149, 2115
EcoT38I GRGCYC 1 cut(s) 538
ErhI CCWWGG 2 cut(s) 149, 2115
FaqI GGGAC 3 cut(s) 353, 789, 1232
FauI CCCGC 2 cut(s) 1174, 1506
FauNDI CATATG 3 cut(s) 1579, 1693, 2103
FbaI TGATCA 2 cut(s) 82, 250
FokI GGATG 4 cut(s) 143, 581, 638, 946
FriOI GRGCYC 1 cut(s) 538
FspBI CTAG 5 cut(s) 303, 1098, 1121, 1500, 2159
GlaI GCGC 2 cut(s) 927, 1394
GsuI CTGGAG 1 cut(s) 921
HaeII RGCGCY 1 cut(s) 929
HaeIII GGCC 9 cut(s) 579, 983, 1165, 1292, 1398, 1443, 1648, 1953, 1976
HapII CCGG 6 cut(s) 128, 1162, 1445, 1536, 1680, 1701
HhaI GCGC 2 cut(s) 928, 1395
Hin6I GCGC 2 cut(s) 926, 1393
HinP1I GCGC 2 cut(s) 926, 1393
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 2 cut(s) 631, 2167
HinfI GANTC 5 cut(s) 413, 431, 557, 1654, 1803
HpaII CCGG 6 cut(s) 128, 1162, 1445, 1536, 1680, 1701
HphI GGTGA 3 cut(s) 152, 1694, 1933
Hpy166II GTNNAC 4 cut(s) 772, 1021, 1199, 2044
Hpy188I TCNGA 9 cut(s) 436, 727, 969, 1140, 1276, 1473, 1808, 1849, 2128
Hpy188III TCNNGA 7 cut(s) 86, 128, 254, 417, 1500, 1536, 1818
Hpy8I GTNNAC 4 cut(s) 772, 1021, 1199, 2044
Hpy99I CGWCG 3 cut(s) 331, 969, 2132
HpyAV CCTTC 6 cut(s) 322, 446, 488, 818, 1890, 2041
HpyCH4III ACNGT 5 cut(s) 893, 913, 1127, 1921, 2193
HpyCH4IV ACGT 2 cut(s) 1912, 1930
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 560, 602, 686, 737, 934, 947, 1190
HpyF3I CTNAG 4 cut(s) 6, 245, 384, 676
HpySE526I ACGT 2 cut(s) 1912, 1930
HspAI GCGC 2 cut(s) 926, 1393
Kpn2I TCCGGA 2 cut(s) 127, 1535
Ksp22I TGATCA 2 cut(s) 82, 250
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 6 cut(s) 14, 219, 472, 533, 902, 2014
LweI GCATC 6 cut(s) 742, 924, 1057, 1225, 1738, 2192
MabI ACCWGGT 1 cut(s) 649
MaeI CTAG 5 cut(s) 303, 1098, 1121, 1500, 2159
MaeII ACGT 2 cut(s) 1912, 1930
MfeI CAATTG 2 cut(s) 756, 834
MflI RGATCY 5 cut(s) 1271, 1318, 1357, 1697, 1867
MhlI GDGCHC 3 cut(s) 538, 722, 1201
MlsI TGGCCA 1 cut(s) 1292
MluNI TGGCCA 1 cut(s) 1292
MlyI GAGTC 3 cut(s) 407, 566, 1812
MmeI TCCRAC 4 cut(s) 340, 947, 2056, 2151
Mox20I TGGCCA 1 cut(s) 1292
MroI TCCGGA 2 cut(s) 127, 1535
MroXI GAANNNNTTC 1 cut(s) 1025
MscI TGGCCA 1 cut(s) 1292
MseI TTAA 5 cut(s) 1425, 1620, 1770, 2145, 2200
Msp20I TGGCCA 1 cut(s) 1292
MspA1I CMGCKG 2 cut(s) 1169, 1364
MspI CCGG 6 cut(s) 128, 1162, 1445, 1536, 1680, 1701
MspR9I CCNGG 6 cut(s) 651, 900, 1445, 1446, 1680, 1734
MunI CAATTG 2 cut(s) 756, 834
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 3 cut(s) 651, 900, 1734
MvnI CGCG 2 cut(s) 1393, 1395
MwoI GCNNNNNNNGC 8 cut(s) 69, 560, 602, 686, 737, 934, 947, 1190
NciI CCSGG 3 cut(s) 1445, 1446, 1680
NdeI CATATG 3 cut(s) 1579, 1693, 2103
NlaIV GGNNCC 5 cut(s) 586, 672, 1540, 1699, 2016
NmuCI GTSAC 5 cut(s) 120, 1157, 1450, 1486, 1921
PagI TCATGA 1 cut(s) 85
PasI CCCWGGG 1 cut(s) 1733
PciSI GCTCTTC 1 cut(s) 727
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 1025
PfeI GAWTC 2 cut(s) 431, 1654
PflFI GACNNNGTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 898
PleI GAGTC 3 cut(s) 407, 565, 1811
PpsI GAGTC 3 cut(s) 407, 565, 1811
Ppu21I YACGTR 1 cut(s) 1931
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 3 cut(s) 649, 898, 1732
PspGI CCWGG 3 cut(s) 649, 898, 1732
PspN4I GGNNCC 5 cut(s) 586, 672, 1540, 1699, 2016
PspPI GGNCC 5 cut(s) 896, 1164, 1397, 1442, 2124
PstI CTGCAG 2 cut(s) 886, 1759
PstNI CAGNNNCTG 2 cut(s) 560, 1847
PsuI RGATCY 5 cut(s) 1271, 1318, 1357, 1697, 1867
PsyI GACNNNGTC 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 4 cut(s) 143, 655, 910, 1523
RsaNI GTAC 4 cut(s) 142, 654, 909, 1522
SacI GAGCTC 1 cut(s) 538
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 5 cut(s) 1425, 1620, 1770, 2145, 2200
Sau96I GGNCC 5 cut(s) 896, 1164, 1397, 1442, 2124
ScaI AGTACT 1 cut(s) 143
SchI GAGTC 3 cut(s) 407, 566, 1812
ScrFI CCNGG 6 cut(s) 651, 900, 1445, 1446, 1680, 1734
SduI GDGCHC 3 cut(s) 538, 722, 1201
SexAI ACCWGGT 1 cut(s) 649
SfaNI GCATC 6 cut(s) 742, 924, 1057, 1225, 1738, 2192
SfcI CTRYAG 6 cut(s) 711, 828, 882, 1638, 1755, 1839
SinI GGWCC 2 cut(s) 896, 2124
SmaI CCCGGG 1 cut(s) 1446
SmlI CTYRAG 4 cut(s) 353, 1323, 1995, 2019
SmoI CTYRAG 4 cut(s) 353, 1323, 1995, 2019
SnaBI TACGTA 1 cut(s) 1931
SsiI CCGC 3 cut(s) 1167, 1395, 1513
SspMI CTAG 5 cut(s) 303, 1098, 1121, 1500, 2159
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 6 cut(s) 649, 898, 1443, 1444, 1678, 1732
StyI CCWWGG 2 cut(s) 149, 2115
TaaI ACNGT 5 cut(s) 893, 913, 1127, 1921, 2193
TaiI ACGT 2 cut(s) 1915, 1933
TaqI TCGA 4 cut(s) 165, 370, 1189, 1310
TatI WGTACW 2 cut(s) 141, 1521
TauI GCSGC 1 cut(s) 1398
TfiI GAWTC 2 cut(s) 431, 1654
Tru1I TTAA 5 cut(s) 1425, 1620, 1770, 2145, 2200
Tru9I TTAA 5 cut(s) 1425, 1620, 1770, 2145, 2200
TscAI CASTG 6 cut(s) 253, 385, 598, 898, 1023, 1056
TseFI GTSAC 5 cut(s) 120, 1157, 1450, 1486, 1921
Tsp45I GTSAC 5 cut(s) 120, 1157, 1450, 1486, 1921
TspGWI ACGGA 5 cut(s) 283, 597, 654, 2029, 2145
TspMI CCCGGG 1 cut(s) 1444
TspRI CASTG 6 cut(s) 253, 385, 598, 898, 1023, 1056
Tth111I GACNNNGTC 1 cut(s) 331
VneI GTGCAC 1 cut(s) 1197
VpaK11BI GGWCC 2 cut(s) 896, 2124
XapI RAATTY 4 cut(s) 505, 1407, 1685, 1960
XbaI TCTAGA 1 cut(s) 1499
XmaI CCCGGG 1 cut(s) 1444
XmnI GAANNNNTTC 1 cut(s) 1025
XspI CTAG 5 cut(s) 303, 1098, 1121, 1500, 2159
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.