FvH4_7g02830

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000257
Physical Location & Seq
Reverse (-)
3156479 .. 3158678
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02830.t1

Sequence Viewer

Length: 2106 bp
ATGGCTAAGCATGGAGCTATCCTATTTTCTTATATTTTCCCCAGTTTCATACTTTTGAGCATCTTCTTGTGCAAGACCATCGATGAAGACAGAAAGGTCCATATTGTGTACCTGGGTTCACTTCCTGATAATGTGGTGTACTCACCATTGTCTCACCACCTTGGATTACTCGAAAAAGTTCTCGAGAGCAACTCCGCTGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCAAAGCTCACTGACCGAGAAAGAGAGAGGCTTGCTAACATGAAAGAAGTAGTCTCTGTCTTTCCCAGCAGAATGCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTCTCAATGAGCAAACAAAACGGAATGCAGGTGATGAGAGTGACACCATCATTGGTGTCATTGACTCTGGAATTTGGCCCGAGTCAGAGAGCTTTAAAGATGATGGTTTCAGTCCTCCTCCAAAGAAGTGGAAAGGTGCTTGTGAAGGAGGCAAAAATTTCACCTGCAACAAGAAGCTAATTGGAGCTCGGTTTTATGGTTCATTCACAGATTCTGCAAGGGATGAAAGAGGCCATGGAACCCACACAGCCTCAACTGCAGCAGGCAATGCTGTAAAGGATGTGAGCTTTTATGGACTAGCAAATGGTACCGCAAGAGGAGGGATTCCCACAGCAAGGATTGCTTCTTATAAAGTCTGTGGTAATGTAGGGTGTCCTTCTGAGGCTATATTGGCTGCTTTTGATGATGCTATTGCCGATGGAGTTGACATCATTACAATTTCATTAGGAAGCCAGGACGTAATTTCATTACAGAATGATCCTATTGCTATCGGTGCTTTTCATGCAATGACAAAAGGAATACTTACATCAAATTCTGCAGGCAACAGTGGTCCTTCTAATGCAACAGTTGCAAGTGTAGCACCATGGATACTTACAGCTGCAGCAAGTAGTACTGATCGTAGAATCTTTGACAAGGTTATTCTCGGAAATGGGACGGCAATTGTTGGGGCTTCAGTGAACACTTTCAAACTAAATGGGACAAGTTTCCCATTGATATATGGAAAAGAGGCTTCAAGTCAAAGTTGCTCTGAGTTAGAAGCTGGGAACTGTCTAGCAGGCTGCTTAGAAAGTGGTTTAGTTAAGGGAAAGGTTGTGTTATGTGACCTGGATATTGGACTCAATATGGCATACCGCTCTGGAGCACTTGGTTCGATTGTGAGAGTTGATTGGGATGATGATTCTCAAATTTTACCCTTTCCTGCAACAACTTTGAGTGACAAAGAGTATAATGTGATCAAGTCTTATTACAAGAACTCCACAAGAAATGCTCGAGTAAATATACTAAGAAGTGAAGTAGAAAGGGATGCTGCTGCGCCTCTTGTTGCTTCCTTCTCTTCACGTGGACCAAATTCAATTCTACCAGAAATTATCAAACCAGATATAAGCGCCCCAGGGGTAAGTATTCTGGCTGCCTTTTCACCTATTGCTTCCATTACGGACAATCCTCAAGACACAAGGCATGTAAAATACAGTATACTATCTGGAACCTCCATGTCCTGCCCGCACGTCGCTGGTGCAGCTGCATATGTTAAAACATTCCATCCTGATTGGTCTCCGGCAACCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATGTTTCCTACAGTAGTCATAATGCCGCTGGTGAATTTGCATATGGATCTGGACATATCAATCCTGTCAGAGCTATAGACCCGGGGCTTGTGTATGAAGCTTCCAAGGAAGACTACATACAGTTGCTCTGCCTGGTCTTGGACCAGGCCAGTGTTAGACTTATAGCAGGAGATAACAGCACTTGCTCTACCAAAAAAGGATCTTTACAGGATCACAATTACCCTTCACTGGCCGCCGTTGTCACACCAAACAAATCTTTTTCAGTCACATTTCACAGAACACTCAAGAATGTTGGCCCTGCATTACAATGCCAAAATATTGGCCAACTCTACTCAAATGAACAACAGGCACATGTGTCTGCATCGCTGGTGTGGTCTGATGAAATTCATAGCGTTAGAAGTCCAATTGTTATCCACAAAAAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

702

Amino Acids

75.03

Weight (kDa)

7.25

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 109 8.9e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 133 - 563 1.5e-43 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AarI CACCTGC 2 cut(s) 377, 530
AasI GACNNNNNNGTC 1 cut(s) 95
Acc36I ACCTGC 2 cut(s) 377, 530
Acc65I GGTACC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 1212
AccI GTMKAC 1 cut(s) 1552
AciI CCGC 6 cut(s) 195, 669, 1210, 1580, 1704, 1911
AclWI GGATC 4 cut(s) 830, 1732, 1885, 1896
AcoI YGGCCR 2 cut(s) 1908, 1999
AcsI RAATTY 8 cut(s) 202, 429, 514, 889, 1263, 1426, 1712, 2061
AcuI CTGAAG 1 cut(s) 1014
AcvI CACGTG 1 cut(s) 1418
AfaI GTAC 4 cut(s) 110, 140, 667, 970
AfiI CCNNNNNNNGG 3 cut(s) 693, 1816, 1906
AflIII ACRYGT 1 cut(s) 2029
AgsI TTSAA 3 cut(s) 1045, 1092, 1431
AjiI CACGTC 1 cut(s) 1585
AjnI CCWGG 6 cut(s) 111, 810, 1182, 1468, 1809, 1821
Alw21I GWGCWC 2 cut(s) 547, 1222
Alw26I GTCTC 4 cut(s) 156, 304, 365, 1635
AlwI GGATC 4 cut(s) 830, 1732, 1885, 1896
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 4 cut(s) 182, 437, 1347, 1759
AoxI GGCC 6 cut(s) 434, 589, 1824, 1908, 1972, 1999
ApoI RAATTY 8 cut(s) 202, 429, 514, 889, 1263, 1426, 1712, 2061
Asp700I GAANNNNTTC 2 cut(s) 177, 1040
Asp718I GGTACC 1 cut(s) 665
AspLEI GCGC 2 cut(s) 1393, 1466
AspS9I GGNCC 6 cut(s) 97, 435, 908, 1421, 1819, 1973
AsuC2I CCSGG 2 cut(s) 1760, 1761
AsuHPI GGTGA 6 cut(s) 135, 146, 401, 511, 1488, 1721
AvaI CYCGRG 4 cut(s) 182, 437, 1347, 1759
AvaII GGWCC 4 cut(s) 97, 908, 1421, 1819
BalI TGGCCA 1 cut(s) 2001
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 547
BarI GAAGNNNNNNTAC 2 cut(s) 1779, 1811
BbrPI CACGTG 1 cut(s) 1418
BbsI GAAGAC 2 cut(s) 93, 1794
Bbv12I GWGCWC 2 cut(s) 547, 1222
BccI CCATC 7 cut(s) 86, 333, 413, 455, 770, 1626, 1649
BceAI ACGGC 2 cut(s) 1029, 1898
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 1209, 1243
BciT130I CCWGG 6 cut(s) 113, 812, 1184, 1470, 1811, 1823
BciVI GTATCC 1 cut(s) 939
BclI TGATCA 1 cut(s) 1311
BcnI CCSGG 2 cut(s) 1760, 1761
BcoDI GTCTC 4 cut(s) 156, 304, 365, 1635
BfaI CTAG 2 cut(s) 656, 1130
BfmI CTRYAG 6 cut(s) 615, 894, 957, 1662, 1687, 1752
BfoI RGCGCY 1 cut(s) 1467
BfuAI ACCTGC 2 cut(s) 377, 530
BfuI GTATCC 1 cut(s) 939
BglII AGATCT 1 cut(s) 341
BlpI GCTNAGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 970
Bme1390I CCNGG 8 cut(s) 113, 812, 1184, 1470, 1760, 1761, 1811, 1823
Bme18I GGWCC 4 cut(s) 97, 908, 1421, 1819
BmeT110I CYCGRG 4 cut(s) 182, 437, 1347, 1759
BmgBI CACGTC 1 cut(s) 1585
BmgT120I GGNCC 6 cut(s) 97, 435, 908, 1421, 1819, 1973
BmiI GGNNCC 3 cut(s) 598, 667, 1564
BmrFI CCNGG 8 cut(s) 113, 812, 1184, 1470, 1760, 1761, 1811, 1823
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 4 cut(s) 69, 754, 1372, 2048
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 93, 1794
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpmI CTGGAG 1 cut(s) 1236
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 1509, 1946
BpuMI CCSGG 2 cut(s) 1760, 1761
Bsa29I ATCGAT 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 1418
BsaI GGTCTC 2 cut(s) 365, 1635
BsaJI CCNNGG 9 cut(s) 112, 160, 592, 941, 1468, 1469, 1759, 1760, 1782
BsaXI ACNNNNNCTCC 6 cut(s) 220, 250, 1316, 1346, 1654, 1684
Bsc4I CCNNNNNNNGG 3 cut(s) 693, 1816, 1906
Bse1I ACTGG 3 cut(s) 42, 1827, 1911
Bse3DI GCAATG 2 cut(s) 631, 870
BseBI CCWGG 6 cut(s) 113, 812, 1184, 1470, 1811, 1823
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 9 cut(s) 112, 160, 592, 941, 1468, 1469, 1759, 1760, 1782
BseGI GGATG 6 cut(s) 325, 586, 643, 1255, 1387, 1618
BseLI CCNNNNNNNGG 3 cut(s) 693, 1816, 1906
BseMI GCAATG 2 cut(s) 631, 870
BseMII CTCAG 3 cut(s) 344, 729, 1098
BseNI ACTGG 3 cut(s) 42, 1827, 1911
BseRI GAGGAG 2 cut(s) 465, 690
BseYI CCCAGC 2 cut(s) 311, 1118
BsgI GTGCAG 1 cut(s) 1614
BshFI GGCC 6 cut(s) 436, 591, 1826, 1910, 1974, 2001
BshNI GGYRCC 1 cut(s) 665
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 2 cut(s) 547, 1222
BsiHKCI CYCGRG 4 cut(s) 182, 437, 1347, 1759
BsiSI CCGG 2 cut(s) 1634, 1760
BslFI GGGAC 3 cut(s) 362, 1024, 1069
BslI CCNNNNNNNGG 3 cut(s) 693, 1816, 1906
BsmAI GTCTC 4 cut(s) 156, 304, 365, 1635
BsmFI GGGAC 3 cut(s) 362, 1024, 1069
BsmI GAATGC 2 cut(s) 324, 388
BsnI GGCC 6 cut(s) 436, 591, 1826, 1910, 1974, 2001
Bso31I GGTCTC 2 cut(s) 365, 1635
BsoBI CYCGRG 4 cut(s) 182, 437, 1347, 1759
Bsp1286I GDGCHC 2 cut(s) 547, 1222
Bsp143I GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 592, 941
BspACI CCGC 6 cut(s) 195, 669, 1210, 1580, 1704, 1911
BspANI GGCC 6 cut(s) 436, 591, 1826, 1910, 1974, 2001
BspCNI CTCAG 3 cut(s) 343, 730, 1099
BspDI ATCGAT 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 598, 667, 1564
BspMAI CTGCAG 3 cut(s) 619, 898, 961
BspMI ACCTGC 2 cut(s) 377, 530
BspPI GGATC 4 cut(s) 830, 1732, 1885, 1896
BspT107I GGYRCC 1 cut(s) 665
BspTNI GGTCTC 2 cut(s) 365, 1635
BsrBI CCGCTC 1 cut(s) 1212
BsrDI GCAATG 2 cut(s) 631, 870
BsrI ACTGG 3 cut(s) 42, 1827, 1911
BssECI CCNNGG 9 cut(s) 112, 160, 592, 941, 1468, 1469, 1759, 1760, 1782
BssMI GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
BssNAI GTATAC 1 cut(s) 1553
BssT1I CCWWGG 4 cut(s) 160, 592, 941, 1782
Bst1107I GTATAC 1 cut(s) 1553
Bst2UI CCWGG 6 cut(s) 113, 812, 1184, 1470, 1811, 1823
Bst4CI ACNGT 6 cut(s) 905, 925, 1127, 1550, 1691, 1800
Bst6I CTCTTC 1 cut(s) 1417
BstAPI GCANNNNNTGC 3 cut(s) 626, 698, 926
BstBAI YACGTR 1 cut(s) 1418
BstC8I GCNNGC 5 cut(s) 279, 622, 898, 1135, 1580
BstDEI CTNAG 6 cut(s) 6, 330, 738, 1107, 1141, 1361
BstDSI CCRYGG 2 cut(s) 592, 941
BstF5I GGATG 6 cut(s) 325, 586, 643, 1255, 1387, 1618
BstH2I RGCGCY 1 cut(s) 1467
BstHHI GCGC 2 cut(s) 1393, 1466
BstKTI GATC 7 cut(s) 344, 838, 976, 1314, 1727, 1880, 1891
BstMAI GTCTC 4 cut(s) 156, 304, 365, 1635
BstMBI GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
BstMWI GCNNNNNNNGC 9 cut(s) 614, 626, 698, 749, 851, 860, 926, 935, 1595
BstNI CCWGG 6 cut(s) 113, 812, 1184, 1470, 1811, 1823
BstNSI RCATGY 2 cut(s) 1541, 2033
BstSCI CCNGG 8 cut(s) 111, 810, 1182, 1468, 1758, 1759, 1809, 1821
BstSFI CTRYAG 6 cut(s) 615, 894, 957, 1662, 1687, 1752
BstV2I GAAGAC 2 cut(s) 93, 1794
BstX2I RGATCY 3 cut(s) 341, 1724, 1877
BstXI CCANNNNNNTGG 2 cut(s) 486, 1997
BstYI RGATCY 3 cut(s) 341, 1724, 1877
BstZ17I GTATAC 1 cut(s) 1553
Bsu15I ATCGAT 1 cut(s) 81
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 6 cut(s) 436, 591, 1826, 1910, 1974, 2001
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 2 cut(s) 592, 941
BtgZI GCGATG 1 cut(s) 2025
BtrI CACGTC 1 cut(s) 1585
BtsCI GGATG 6 cut(s) 325, 586, 643, 1255, 1387, 1618
BtsIMutI CAGTG 6 cut(s) 241, 255, 910, 1038, 1834, 1904
BveI ACCTGC 2 cut(s) 377, 530
Cac8I GCNNGC 5 cut(s) 279, 622, 898, 1135, 1580
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 2 cut(s) 1393, 1466
Cfr13I GGNCC 6 cut(s) 97, 435, 908, 1421, 1819, 1973
Cfr9I CCCGGG 1 cut(s) 1759
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 4 cut(s) 109, 139, 666, 969
CviAII CATG 9 cut(s) 11, 286, 593, 860, 942, 1538, 1570, 1675, 2030
CviQI GTAC 4 cut(s) 109, 139, 666, 969
DdeI CTNAG 6 cut(s) 6, 330, 738, 1107, 1141, 1361
DpnI GATC 7 cut(s) 343, 837, 975, 1313, 1726, 1879, 1890
DpnII GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
DraI TTTAAA 1 cut(s) 454
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 2 cut(s) 1908, 1999
Eam1104I CTCTTC 1 cut(s) 1417
EarI CTCTTC 1 cut(s) 1417
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 4 cut(s) 160, 592, 941, 1782
Eco24I GRGCYC 1 cut(s) 547
Eco31I GGTCTC 2 cut(s) 365, 1635
Eco47I GGWCC 4 cut(s) 97, 908, 1421, 1819
Eco53kI GAGCTC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 1014
Eco72I CACGTG 1 cut(s) 1418
Eco88I CYCGRG 4 cut(s) 182, 437, 1347, 1759
EcoICRI GAGCTC 1 cut(s) 545
EcoRII CCWGG 6 cut(s) 111, 810, 1182, 1468, 1809, 1821
EcoT14I CCWWGG 4 cut(s) 160, 592, 941, 1782
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 4 cut(s) 160, 592, 941, 1782
FaeI CATG 9 cut(s) 14, 289, 596, 863, 945, 1541, 1573, 1678, 2033
FalI AAGNNNNNCTT 4 cut(s) 685, 717, 864, 896
FaqI GGGAC 3 cut(s) 362, 1024, 1069
FatI CATG 9 cut(s) 10, 285, 592, 859, 941, 1537, 1569, 1674, 2029
FauI CCCGC 1 cut(s) 1587
FauNDI CATATG 2 cut(s) 1603, 1720
FbaI TGATCA 1 cut(s) 1311
FblI GTMKAC 1 cut(s) 1552
FokI GGATG 6 cut(s) 312, 593, 650, 1262, 1394, 1605
FriOI GRGCYC 1 cut(s) 547
FspBI CTAG 2 cut(s) 656, 1130
GlaI GCGC 2 cut(s) 1392, 1465
GsaI CCCAGC 2 cut(s) 315, 1122
GsuI CTGGAG 1 cut(s) 1236
HaeII RGCGCY 1 cut(s) 1467
HaeIII GGCC 6 cut(s) 436, 591, 1826, 1910, 1974, 2001
HapII CCGG 2 cut(s) 1634, 1760
HhaI GCGC 2 cut(s) 1393, 1466
Hin1II CATG 9 cut(s) 14, 289, 596, 863, 945, 1541, 1573, 1678, 2033
Hin6I GCGC 2 cut(s) 1391, 1464
HinP1I GCGC 2 cut(s) 1391, 1464
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HindIII AAGCTT 1 cut(s) 1776
HinfI GANTC 7 cut(s) 422, 440, 569, 682, 981, 1194, 1256
HpaII CCGG 2 cut(s) 1634, 1760
HphI GGTGA 6 cut(s) 135, 146, 401, 511, 1488, 1721
Hpy166II GTNNAC 7 cut(s) 109, 119, 139, 784, 1036, 1421, 1553
Hpy188I TCNGA 7 cut(s) 333, 445, 739, 1004, 1108, 1748, 2056
Hpy8I GTNNAC 7 cut(s) 109, 119, 139, 784, 1036, 1421, 1553
Hpy99I CGWCG 1 cut(s) 1589
HpyAV CCTTC 5 cut(s) 497, 744, 921, 1417, 1911
HpyCH4III ACNGT 6 cut(s) 905, 925, 1127, 1550, 1691, 1800
HpyCH4IV ACGT 3 cut(s) 816, 1417, 1584
HpyF10VI GCNNNNNNNGC 9 cut(s) 614, 626, 698, 749, 851, 860, 926, 935, 1595
HpyF3I CTNAG 6 cut(s) 6, 330, 738, 1107, 1141, 1361
HpySE526I ACGT 3 cut(s) 816, 1417, 1584
Hsp92II CATG 9 cut(s) 14, 289, 596, 863, 945, 1541, 1573, 1678, 2033
HspAI GCGC 2 cut(s) 1391, 1464
KpnI GGTACC 1 cut(s) 669
Ksp22I TGATCA 1 cut(s) 1311
Kzo9I GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
LmnI GCTCC 4 cut(s) 14, 327, 542, 1217
LweI GCATC 4 cut(s) 69, 754, 1372, 2048
MaeI CTAG 2 cut(s) 656, 1130
MaeII ACGT 3 cut(s) 816, 1417, 1584
MaeIII GTNAC 6 cut(s) 218, 398, 1178, 1292, 1918, 1942
MalI GATC 7 cut(s) 343, 837, 975, 1313, 1726, 1879, 1890
MbiI CCGCTC 1 cut(s) 1212
MboI GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
MboII GAAGA 4 cut(s) 55, 98, 1404, 1799
MfeI CAATTG 2 cut(s) 1017, 2082
MflI RGATCY 3 cut(s) 341, 1724, 1877
MhlI GDGCHC 2 cut(s) 547, 1222
MlsI TGGCCA 1 cut(s) 2001
MluNI TGGCCA 1 cut(s) 2001
MlyI GAGTC 3 cut(s) 416, 449, 1188
Mox20I TGGCCA 1 cut(s) 2001
MroXI GAANNNNTTC 2 cut(s) 177, 1040
MscI TGGCCA 1 cut(s) 2001
MseI TTAA 3 cut(s) 453, 1158, 1608
MslI CAYNNNNRTG 1 cut(s) 1984
Msp20I TGGCCA 1 cut(s) 2001
MspA1I CMGCKG 4 cut(s) 197, 956, 1598, 1706
MspI CCGG 2 cut(s) 1634, 1760
MspR9I CCNGG 8 cut(s) 113, 812, 1184, 1470, 1760, 1761, 1811, 1823
MunI CAATTG 2 cut(s) 1017, 2082
Mva1269I GAATGC 2 cut(s) 324, 388
MvaI CCWGG 6 cut(s) 113, 812, 1184, 1470, 1811, 1823
MwoI GCNNNNNNNGC 9 cut(s) 614, 626, 698, 749, 851, 860, 926, 935, 1595
NciI CCSGG 2 cut(s) 1760, 1761
NcoI CCATGG 2 cut(s) 592, 941
NdeI CATATG 2 cut(s) 1603, 1720
NdeII GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
NlaIII CATG 9 cut(s) 14, 289, 596, 863, 945, 1541, 1573, 1678, 2033
NlaIV GGNNCC 3 cut(s) 598, 667, 1564
NmuCI GTSAC 5 cut(s) 398, 1178, 1292, 1918, 1942
NspI RCATGY 2 cut(s) 1541, 2033
PaeR7I CTCGAG 2 cut(s) 182, 1347
PaqCI CACCTGC 2 cut(s) 377, 530
PasI CCCWGGG 1 cut(s) 1469
PciI ACATGT 1 cut(s) 2029
PctI GAATGC 2 cut(s) 324, 388
PdmI GAANNNNTTC 2 cut(s) 177, 1040
PfeI GAWTC 4 cut(s) 569, 682, 981, 1256
PleI GAGTC 3 cut(s) 416, 448, 1188
PmaCI CACGTG 1 cut(s) 1418
PmlI CACGTG 1 cut(s) 1418
PpsI GAGTC 3 cut(s) 416, 448, 1188
Ppu21I YACGTR 1 cut(s) 1418
PscI ACATGT 1 cut(s) 2029
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 547
Psp6I CCWGG 6 cut(s) 111, 810, 1182, 1468, 1809, 1821
PspCI CACGTG 1 cut(s) 1418
PspFI CCCAGC 2 cut(s) 311, 1118
PspGI CCWGG 6 cut(s) 111, 810, 1182, 1468, 1809, 1821
PspN4I GGNNCC 3 cut(s) 598, 667, 1564
PspPI GGNCC 6 cut(s) 97, 435, 908, 1421, 1819, 1973
PspXI VCTCGAGB 1 cut(s) 1347
PstI CTGCAG 3 cut(s) 619, 898, 961
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 3 cut(s) 341, 1724, 1877
PvuII CAGCTG 2 cut(s) 956, 1598
RsaI GTAC 4 cut(s) 110, 140, 667, 970
RsaNI GTAC 4 cut(s) 109, 139, 666, 969
RseI CAYNNNNRTG 1 cut(s) 1984
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 3 cut(s) 453, 1158, 1608
Sau3AI GATC 7 cut(s) 341, 835, 973, 1311, 1724, 1877, 1888
Sau96I GGNCC 6 cut(s) 97, 435, 908, 1421, 1819, 1973
ScaI AGTACT 1 cut(s) 970
SchI GAGTC 3 cut(s) 416, 449, 1188
ScrFI CCNGG 8 cut(s) 113, 812, 1184, 1470, 1760, 1761, 1811, 1823
SduI GDGCHC 2 cut(s) 547, 1222
SfaNI GCATC 4 cut(s) 69, 754, 1372, 2048
SfcI CTRYAG 6 cut(s) 615, 894, 957, 1662, 1687, 1752
Sfr274I CTCGAG 2 cut(s) 182, 1347
SinI GGWCC 4 cut(s) 97, 908, 1421, 1819
SlaI CTCGAG 2 cut(s) 182, 1347
SmaI CCCGGG 1 cut(s) 1761
SmiMI CAYNNNNRTG 1 cut(s) 1984
SmlI CTYRAG 4 cut(s) 182, 1347, 1524, 1961
SmoI CTYRAG 4 cut(s) 182, 1347, 1524, 1961
SsiI CCGC 6 cut(s) 195, 669, 1210, 1580, 1704, 1911
SspI AATATT 1 cut(s) 1996
SspMI CTAG 2 cut(s) 656, 1130
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 8 cut(s) 111, 810, 1182, 1468, 1758, 1759, 1809, 1821
StyI CCWWGG 4 cut(s) 160, 592, 941, 1782
TaaI ACNGT 6 cut(s) 905, 925, 1127, 1550, 1691, 1800
TaiI ACGT 3 cut(s) 819, 1420, 1587
TaqI TCGA 5 cut(s) 81, 171, 183, 1229, 1348
TaqII GACCGA 2 cut(s) 276, 347
TatI WGTACW 2 cut(s) 138, 968
TauI GCSGC 2 cut(s) 1706, 1913
TfiI GAWTC 4 cut(s) 569, 682, 981, 1256
Tru1I TTAA 3 cut(s) 453, 1158, 1608
Tru9I TTAA 3 cut(s) 453, 1158, 1608
TscAI CASTG 6 cut(s) 241, 262, 910, 1038, 1834, 1911
TseFI GTSAC 5 cut(s) 398, 1178, 1292, 1918, 1942
Tsp45I GTSAC 5 cut(s) 398, 1178, 1292, 1918, 1942
TspGWI ACGGA 2 cut(s) 394, 1529
TspMI CCCGGG 1 cut(s) 1759
TspRI CASTG 6 cut(s) 241, 262, 910, 1038, 1834, 1911
VpaK11BI GGWCC 4 cut(s) 97, 908, 1421, 1819
XapI RAATTY 8 cut(s) 202, 429, 514, 889, 1263, 1426, 1712, 2061
XceI RCATGY 2 cut(s) 1541, 2033
XhoI CTCGAG 2 cut(s) 182, 1347
XmaI CCCGGG 1 cut(s) 1759
XmiI GTMKAC 1 cut(s) 1552
XmnI GAANNNNTTC 2 cut(s) 177, 1040
XspI CTAG 2 cut(s) 656, 1130
ZrmI AGTACT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.