Rh1AG054700

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000257
Physical Location & Seq
Reverse (-)
9022800 .. 9023934
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG054700.1

Sequence Viewer

Length: 1074 bp
ATGGCTAAGCATGGAGCATTCCTATTCTCTTATGCTTTCTCTACTCTCATACTTACTATGAGCTTATTGTGCAACGCCATTGATCATGAAGATAGAAAGGTTCATATAGTGTATCTGGGGTCACTTCCTGATGATGAGGAGTACTCGCCAAGGTCTCATCACCTCGATATAGTACAAAGAGTTGTTGATGGCAGTTCTGCTGAAAGTTGCTTGATAAGAAGTTACCAAAGGAGCTTCAATGGATTTGCTGCAAAGCTCAGTGATCATGAAAAAGAGAAGATTGCTAACATGGAGGAGGTGATCTCTGTCTTTCCTAGCAGAAACTTCCAACTTCAAACAACGAGGTCATGGGACTTTATGGGTCTCAAGCTGTCAAGTCCTCGAAAAGTCACTGCTGAGAGTAATGTTATTGTTGCTGTGATTGACACTGGAATTGACCCTGATTCGGAGAGCTTTAGAGATGAAGGTTTTGGTGCTGCTCCTATGAAGTGGAAAGGTGTTTGTGAAGGTGGCGAAAATTTCAGTTGCAACAACAAGATAATTGGAGCTCGTTTTTACGAACCAGCAAAGTCTGCAAGGGATGAAGACGGCCATGGAACCCACACTGCCTCAACAGTAGCAGGGAATGAGGTAAGGGATGTAAGCTTTTACGGATTAGAACCAGGTACAGCTAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTCTGTGGGGCTACAGGGTGCTCTTCAGAGGCTATATTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTTGACATTGTGTCAGTCTCACTTGGAAGTGCAAGTCCCCTTCCTTTTGATAATGATCCTATAGCAATTGGTTCTTATCATGCAATGAAGAGAGGGATATTGACATCAAACTCTGCAGGCAACAGTGGTCCTGGAGCTGGTACTGTAACAAGTGTAGCGCCGTGGATGCTTGCAGTTGCAGCAAGTAGCAAAGATCGTCGGATCATTGACAAGGCCGTTCTTGGAAGTAGGACACTAGTAGGGGCTTCAGTGAACTCTTTCATATTGAAAGGAGTTGTGCAGTTGGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

357

Amino Acids

37.98

Weight (kDa)

5.74

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 111 3.8e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 322 9.2e-24 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 830, 989
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 2 cut(s) 720, 1011
AfaI GTAC 4 cut(s) 143, 174, 667, 922
AfiI CCNNNNNNNGG 2 cut(s) 445, 917
AgsI TTSAA 3 cut(s) 238, 335, 1048
AhdI GACNNNNNGTC 1 cut(s) 790
AhlI ACTAGT 1 cut(s) 1015
AjnI CCWGG 2 cut(s) 661, 910
AluBI AGCT 9 cut(s) 63, 234, 256, 370, 453, 548, 645, 671, 917
AluI AGCT 9 cut(s) 63, 234, 256, 370, 453, 548, 645, 671, 917
Alw21I GWGCWC 2 cut(s) 550, 734
Alw26I GTCTC 3 cut(s) 159, 368, 802
AlwI GGATC 2 cut(s) 830, 989
AoxI GGCC 2 cut(s) 589, 993
ApeKI GCWGC 5 cut(s) 248, 476, 701, 752, 959
ApoI RAATTY 1 cut(s) 517
Asp700I GAANNNNTTC 1 cut(s) 1037
AspLEI GCGC 1 cut(s) 940
AspS9I GGNCC 1 cut(s) 908
AsuHPI GGTGA 2 cut(s) 152, 310
AvaII GGWCC 1 cut(s) 908
BanII GRGCYC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 2 cut(s) 550, 734
BbvCI CCTCAGC 1 cut(s) 688
BbvI GCAGC 5 cut(s) 235, 463, 688, 739, 971
BccI CCATC 2 cut(s) 182, 770
BceAI ACGGC 3 cut(s) 604, 925, 980
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 2 cut(s) 663, 912
BclI TGATCA 2 cut(s) 82, 262
BcoDI GTCTC 3 cut(s) 159, 368, 802
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 3 cut(s) 315, 672, 1016
BfmI CTRYAG 3 cut(s) 723, 840, 894
BfoI RGCGCY 1 cut(s) 941
BisI GCNGC 5 cut(s) 249, 477, 702, 753, 960
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 5 cut(s) 250, 478, 703, 754, 961
BmcAI AGTACT 1 cut(s) 143
Bme1390I CCNGG 2 cut(s) 663, 912
Bme18I GGWCC 1 cut(s) 908
BmeRI GACNNNNNGTC 1 cut(s) 790
BmgT120I GGNCC 1 cut(s) 908
BmiI GGNNCC 2 cut(s) 598, 684
BmrFI CCNGG 2 cut(s) 663, 912
BmsI GCATC 2 cut(s) 754, 936
BpiI GAAGAC 1 cut(s) 591
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 287, 319
BpmI CTGGAG 1 cut(s) 933
Bpu10I CCTNAGC 1 cut(s) 688
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 350
BsaBI GATNNNNATC 1 cut(s) 834
BsaI GGTCTC 2 cut(s) 159, 368
BsaJI CCNNGG 3 cut(s) 149, 592, 941
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 917
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 870
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 2 cut(s) 663, 912
BseDI CCNNGG 3 cut(s) 149, 592, 941
BseGI GGATG 3 cut(s) 586, 643, 951
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 2 cut(s) 445, 917
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 3 cut(s) 271, 387, 702
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 152, 308, 690
BseXI GCAGC 5 cut(s) 235, 463, 688, 739, 971
BshFI GGCC 2 cut(s) 591, 995
BsiHKAI GWGCWC 2 cut(s) 550, 734
BslFI GGGAC 2 cut(s) 365, 801
BslI CCNNNNNNNGG 2 cut(s) 445, 917
BsmAI GTCTC 3 cut(s) 159, 368, 802
BsmFI GGGAC 2 cut(s) 365, 801
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 591, 995
Bso31I GGTCTC 2 cut(s) 159, 368
Bsp1286I GDGCHC 2 cut(s) 550, 734
Bsp143I GATC 6 cut(s) 82, 262, 300, 835, 973, 981
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 592
BspANI GGCC 2 cut(s) 591, 995
BspCNI CTCAG 3 cut(s) 270, 388, 701
BspHI TCATGA 2 cut(s) 85, 265
BspLI GGNNCC 2 cut(s) 598, 684
BspMAI CTGCAG 1 cut(s) 898
BspPI GGATC 2 cut(s) 830, 989
BspQI GCTCTTC 1 cut(s) 739
BspTNI GGTCTC 2 cut(s) 159, 368
BsrDI GCAATG 1 cut(s) 870
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 3 cut(s) 149, 592, 941
BssMI GATC 6 cut(s) 82, 262, 300, 835, 973, 981
BssT1I CCWWGG 2 cut(s) 149, 592
Bst2UI CCWGG 2 cut(s) 663, 912
Bst4CI ACNGT 3 cut(s) 616, 905, 925
Bst6I CTCTTC 2 cut(s) 739, 863
BstAPI GCANNNNNTGC 1 cut(s) 572
BstC8I GCNNGC 2 cut(s) 898, 951
BstDEI CTNAG 5 cut(s) 6, 257, 396, 688, 1071
BstDSI CCRYGG 2 cut(s) 592, 941
BstF5I GGATG 3 cut(s) 586, 643, 951
BstH2I RGCGCY 1 cut(s) 941
BstHHI GCGC 1 cut(s) 940
BstKTI GATC 6 cut(s) 85, 265, 303, 838, 976, 984
BstMAI GTCTC 3 cut(s) 159, 368, 802
BstMBI GATC 6 cut(s) 82, 262, 300, 835, 973, 981
BstMWI GCNNNNNNNGC 6 cut(s) 69, 572, 698, 749, 946, 959
BstNI CCWGG 2 cut(s) 663, 912
BstSCI CCNGG 2 cut(s) 661, 910
BstSFI CTRYAG 3 cut(s) 723, 840, 894
BstV1I GCAGC 5 cut(s) 235, 463, 688, 739, 971
BstV2I GAAGAC 1 cut(s) 591
BsuRI GGCC 2 cut(s) 591, 995
BtgI CCRYGG 2 cut(s) 592, 941
BtsCI GGATG 3 cut(s) 586, 643, 951
BtsI GCAGTG 2 cut(s) 390, 603
BtsIMutI CAGTG 6 cut(s) 265, 390, 426, 603, 910, 1035
Cac8I GCNNGC 2 cut(s) 898, 951
CciI TCATGA 2 cut(s) 85, 265
CfoI GCGC 1 cut(s) 940
Cfr13I GGNCC 1 cut(s) 908
CsiI ACCWGGT 1 cut(s) 661
Csp6I GTAC 4 cut(s) 142, 173, 666, 921
CviAII CATG 7 cut(s) 11, 86, 266, 289, 348, 593, 860
CviQI GTAC 4 cut(s) 142, 173, 666, 921
DdeI CTNAG 5 cut(s) 6, 257, 396, 688, 1071
DpnI GATC 6 cut(s) 84, 264, 302, 837, 975, 983
DpnII GATC 6 cut(s) 82, 262, 300, 835, 973, 981
DriI GACNNNNNGTC 1 cut(s) 790
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 2 cut(s) 739, 863
Eam1105I GACNNNNNGTC 1 cut(s) 790
EarI CTCTTC 2 cut(s) 739, 863
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 2 cut(s) 149, 592
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 2 cut(s) 159, 368
Eco47I GGWCC 1 cut(s) 908
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 2 cut(s) 720, 1011
EcoICRI GAGCTC 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 661, 910
EcoT14I CCWWGG 2 cut(s) 149, 592
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 2 cut(s) 149, 592
FaeI CATG 7 cut(s) 14, 89, 269, 292, 351, 596, 863
FaqI GGGAC 2 cut(s) 365, 801
FatI CATG 7 cut(s) 10, 85, 265, 288, 347, 592, 859
FbaI TGATCA 2 cut(s) 82, 262
Fnu4HI GCNGC 5 cut(s) 249, 477, 702, 753, 960
FokI GGATG 3 cut(s) 593, 650, 958
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 5 cut(s) 249, 477, 702, 753, 960
FspBI CTAG 3 cut(s) 315, 672, 1016
GlaI GCGC 1 cut(s) 939
GluI GCNGC 5 cut(s) 249, 477, 702, 753, 960
GsuI CTGGAG 1 cut(s) 933
HaeII RGCGCY 1 cut(s) 941
HaeIII GGCC 2 cut(s) 591, 995
HhaI GCGC 1 cut(s) 940
Hin1II CATG 7 cut(s) 14, 89, 269, 292, 351, 596, 863
Hin6I GCGC 1 cut(s) 938
HinP1I GCGC 1 cut(s) 938
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 1 cut(s) 443
HphI GGTGA 2 cut(s) 152, 310
Hpy166II GTNNAC 2 cut(s) 784, 1033
Hpy188I TCNGA 3 cut(s) 448, 739, 981
Hpy188III TCNNGA 3 cut(s) 86, 128, 266
Hpy8I GTNNAC 2 cut(s) 784, 1033
Hpy99I CGWCG 1 cut(s) 981
HpyAV CCTTC 3 cut(s) 458, 500, 830
HpyCH4III ACNGT 3 cut(s) 616, 905, 925
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 572, 698, 749, 946, 959
HpyF3I CTNAG 5 cut(s) 6, 257, 396, 688, 1071
Hsp92II CATG 7 cut(s) 14, 89, 269, 292, 351, 596, 863
HspAI GCGC 1 cut(s) 938
Ksp22I TGATCA 2 cut(s) 82, 262
Kzo9I GATC 6 cut(s) 82, 262, 300, 835, 973, 981
LguI GCTCTTC 1 cut(s) 739
LmnI GCTCC 5 cut(s) 14, 231, 484, 545, 914
Lsp1109I GCAGC 5 cut(s) 235, 463, 688, 739, 971
LweI GCATC 2 cut(s) 754, 936
MabI ACCWGGT 1 cut(s) 661
MaeI CTAG 3 cut(s) 315, 672, 1016
MaeIII GTNAC 4 cut(s) 120, 221, 388, 925
MalI GATC 6 cut(s) 84, 264, 302, 837, 975, 983
MboI GATC 6 cut(s) 82, 262, 300, 835, 973, 981
MboII GAAGA 5 cut(s) 101, 289, 596, 726, 880
MfeI CAATTG 2 cut(s) 768, 846
MhlI GDGCHC 2 cut(s) 550, 734
MluCI AATT 6 cut(s) 432, 517, 540, 696, 768, 846
MmeI TCCRAC 2 cut(s) 352, 959
MroXI GAANNNNTTC 1 cut(s) 1037
MspR9I CCNGG 2 cut(s) 663, 912
MunI CAATTG 2 cut(s) 768, 846
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 2 cut(s) 663, 912
MwoI GCNNNNNNNGC 6 cut(s) 69, 572, 698, 749, 946, 959
NcoI CCATGG 1 cut(s) 592
NdeII GATC 6 cut(s) 82, 262, 300, 835, 973, 981
NlaIII CATG 7 cut(s) 14, 89, 269, 292, 351, 596, 863
NlaIV GGNNCC 2 cut(s) 598, 684
NmuCI GTSAC 2 cut(s) 120, 388
PagI TCATGA 2 cut(s) 85, 265
PciSI GCTCTTC 1 cut(s) 739
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 910
PkrI GCNGC 5 cut(s) 250, 478, 703, 754, 961
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 2 cut(s) 661, 910
PspGI CCWGG 2 cut(s) 661, 910
PspN4I GGNNCC 2 cut(s) 598, 684
PspPI GGNCC 1 cut(s) 908
PstI CTGCAG 1 cut(s) 898
RsaI GTAC 4 cut(s) 143, 174, 667, 922
RsaNI GTAC 4 cut(s) 142, 173, 666, 921
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 1 cut(s) 739
SatI GCNGC 5 cut(s) 249, 477, 702, 753, 960
Sau3AI GATC 6 cut(s) 82, 262, 300, 835, 973, 981
Sau96I GGNCC 1 cut(s) 908
ScaI AGTACT 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 663, 912
SduI GDGCHC 2 cut(s) 550, 734
SexAI ACCWGGT 1 cut(s) 661
SfaNI GCATC 2 cut(s) 754, 936
SfcI CTRYAG 3 cut(s) 723, 840, 894
SinI GGWCC 1 cut(s) 908
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SpeI ACTAGT 1 cut(s) 1015
Sse9I AATT 6 cut(s) 432, 517, 540, 696, 768, 846
SspMI CTAG 3 cut(s) 315, 672, 1016
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 661, 910
StyI CCWWGG 2 cut(s) 149, 592
TaaI ACNGT 3 cut(s) 616, 905, 925
TaqI TCGA 2 cut(s) 165, 382
TasI AATT 6 cut(s) 432, 517, 540, 696, 768, 846
TatI WGTACW 2 cut(s) 141, 172
TfiI GAWTC 1 cut(s) 443
TscAI CASTG 6 cut(s) 265, 397, 433, 610, 910, 1035
TseFI GTSAC 2 cut(s) 120, 388
TseI GCWGC 5 cut(s) 248, 476, 701, 752, 959
Tsp45I GTSAC 2 cut(s) 120, 388
TspDTI ATGAA 8 cut(s) 92, 102, 282, 477, 500, 597, 881, 1030
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 6 cut(s) 265, 397, 433, 610, 910, 1035
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 3 cut(s) 315, 672, 1016
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.