Rmu_sc0000804.1_g000004

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000804.1
OG0000257
Physical Location & Seq
Reverse (-)
14450 .. 15439
990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000804.1_g000004.1.cds

Sequence Viewer

Length: 990 bp
atggctaagcatgtagctatcctattttcttatgctttccccactcttatactaaccttcaacttcttcctgtgccaagccattgatgaaaatagaacggtccacattgtgtaccttggttcacttcctgataatgaggtgtactcaccaatgtctcaccaccttggtttactccaaaaagttgtcgacagcaactctgcagaaaatttcttgacaagaagttacaaaaggagtttcagtggatttgctgcaaagctcactgaccgggaaagggaaaggcttgctaacatgaaggaagtagtctctgtatttccaagcagaattctccatcctcagacaacaagatcttgggactttatcggtttccatgagaaaataaaacggaatgtcagtgttgagagtgataccattattggtgtcattgacactggaatttggcctgaatcggagagctttaaagatgatggttttggtcctcctcccaagaagtggaaaggtgcttgtgaaggtggccaaaatttcacttgcaacaagaagctaattggagctcggttttacaattcatcttcagaatctgcaagggatgaacaaggccatggaacccacacagcctcaactgcagtagggaacgctataaaggatgtgagcttttatggactagcacaaggtactgcaagaggaggggttccctcagcgagaattgctgcgtataaagtatgtggtgctcttgggtgccctactgatgctatattggctgcctttgatgatgctattgccgatggagttgacatcattacagtttcattaggaaacccggttgcatctttattacagcatgaccctattgctatcggggctttccatgcaatggcaaaggggatacttacatcaaattctgcaggcaacggtggtcctgttgattcaagtgtttcaagtgtagcaccatggatacttacaattgcagcaagtagtactgatcgtaggatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

329

Amino Acids

35.37

Weight (kDa)

6.79

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 732
AccB7I CCANNNNNTGG 2 cut(s) 489, 868
AccI GTMKAC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 5 cut(s) 205, 321, 432, 517, 892
AcuI CTGAAG 1 cut(s) 552
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 4 cut(s) 113, 143, 670, 973
AfiI CCNNNNNNNGG 3 cut(s) 271, 489, 868
AgsI TTSAA 3 cut(s) 61, 924, 933
AluBI AGCT 6 cut(s) 17, 256, 453, 538, 548, 648
AluI AGCT 6 cut(s) 17, 256, 453, 538, 548, 648
Alw21I GWGCWC 2 cut(s) 550, 727
Alw26I GTCTC 2 cut(s) 159, 307
AlwNI CAGNNNCTG 1 cut(s) 575
AoxI GGCC 3 cut(s) 437, 511, 592
ApeKI GCWGC 4 cut(s) 248, 704, 755, 962
ApoI RAATTY 5 cut(s) 205, 321, 432, 517, 892
AspS9I GGNCC 3 cut(s) 100, 473, 911
AsuC2I CCSGG 2 cut(s) 266, 815
AsuHPI GGTGA 2 cut(s) 138, 149
AvaII GGWCC 3 cut(s) 100, 473, 911
BaeGI GKGCMC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 1 cut(s) 732
BanII GRGCYC 1 cut(s) 550
Bbv12I GWGCWC 2 cut(s) 550, 727
BbvCI CCTCAGC 1 cut(s) 691
BbvI GCAGC 4 cut(s) 235, 691, 742, 974
BccI CCATC 3 cut(s) 336, 458, 773
BciVI GTATCC 2 cut(s) 873, 942
BcnI CCSGG 2 cut(s) 266, 815
BcoDI GTCTC 2 cut(s) 159, 307
BfaI CTAG 1 cut(s) 659
BfmI CTRYAG 3 cut(s) 198, 618, 897
BfuI GTATCC 2 cut(s) 873, 942
BglII AGATCT 1 cut(s) 344
BisI GCNGC 4 cut(s) 249, 705, 756, 963
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 4 cut(s) 250, 706, 757, 964
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 2 cut(s) 266, 815
Bme18I GGWCC 3 cut(s) 100, 473, 911
BmgT120I GGNCC 3 cut(s) 100, 473, 911
BmiI GGNNCC 3 cut(s) 601, 687, 734
BmrFI CCNGG 2 cut(s) 266, 815
BmsI GCATC 3 cut(s) 733, 757, 830
BplI GAGNNNNNCTC 2 cut(s) 128, 160
Bpu10I CCTNAGC 1 cut(s) 691
Bpu1102I GCTNAGC 1 cut(s) 6
BpuMI CCSGG 2 cut(s) 266, 815
BsaJI CCNNGG 4 cut(s) 115, 163, 595, 944
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 489, 868
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 873
BseDI CCNNGG 4 cut(s) 115, 163, 595, 944
BseGI GGATG 3 cut(s) 328, 589, 646
BseLI CCNNNNNNNGG 3 cut(s) 271, 489, 868
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 2 cut(s) 347, 705
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 2 cut(s) 468, 693
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 4 cut(s) 235, 691, 742, 974
BshFI GGCC 3 cut(s) 439, 513, 594
BshNI GGYRCC 1 cut(s) 732
BsiHKAI GWGCWC 2 cut(s) 550, 727
BsiSI CCGG 2 cut(s) 265, 815
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 3 cut(s) 271, 489, 868
BsmAI GTCTC 2 cut(s) 159, 307
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 3 cut(s) 439, 513, 594
Bsp1286I GDGCHC 3 cut(s) 550, 727, 737
Bsp143I GATC 3 cut(s) 344, 976, 984
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 595, 944
BspANI GGCC 3 cut(s) 439, 513, 594
BspCNI CTCAG 2 cut(s) 346, 704
BspLI GGNNCC 3 cut(s) 601, 687, 734
BspMAI CTGCAG 3 cut(s) 202, 622, 901
BspT107I GGYRCC 1 cut(s) 732
BsrDI GCAATG 1 cut(s) 873
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 4 cut(s) 115, 163, 595, 944
BssMI GATC 3 cut(s) 344, 976, 984
BssT1I CCWWGG 4 cut(s) 115, 163, 595, 944
Bst4CI ACNGT 3 cut(s) 100, 799, 908
BstC8I GCNNGC 2 cut(s) 282, 901
BstDEI CTNAG 3 cut(s) 6, 333, 691
BstDSI CCRYGG 2 cut(s) 595, 944
BstF5I GGATG 3 cut(s) 328, 589, 646
BstKTI GATC 3 cut(s) 347, 979, 987
BstMAI GTCTC 2 cut(s) 159, 307
BstMBI GATC 3 cut(s) 344, 976, 984
BstMWI GCNNNNNNNGC 5 cut(s) 617, 701, 752, 854, 863
BstNSI RCATGY 1 cut(s) 14
BstSCI CCNGG 2 cut(s) 264, 813
BstSFI CTRYAG 3 cut(s) 198, 618, 897
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 4 cut(s) 235, 691, 742, 974
BstX2I RGATCY 2 cut(s) 344, 984
BstYI RGATCY 2 cut(s) 344, 984
BsuI GTATCC 2 cut(s) 873, 942
BsuRI GGCC 3 cut(s) 439, 513, 594
BtgI CCRYGG 2 cut(s) 595, 944
BtsCI GGATG 3 cut(s) 328, 589, 646
BtsIMutI CAGTG 4 cut(s) 244, 258, 397, 426
Cac8I GCNNGC 2 cut(s) 282, 901
CaiI CAGNNNCTG 1 cut(s) 575
Cfr13I GGNCC 3 cut(s) 100, 473, 911
Csp6I GTAC 4 cut(s) 112, 142, 669, 972
CviAII CATG 7 cut(s) 11, 289, 368, 596, 836, 863, 945
CviQI GTAC 4 cut(s) 112, 142, 669, 972
DdeI CTNAG 3 cut(s) 6, 333, 691
DpnI GATC 3 cut(s) 346, 978, 986
DpnII GATC 3 cut(s) 344, 976, 984
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 4 cut(s) 115, 163, 595, 944
Eco24I GRGCYC 1 cut(s) 550
Eco47I GGWCC 3 cut(s) 100, 473, 911
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 321
EcoT14I CCWWGG 4 cut(s) 115, 163, 595, 944
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 4 cut(s) 115, 163, 595, 944
FaeI CATG 7 cut(s) 14, 292, 371, 599, 839, 866, 948
FaqI GGGAC 1 cut(s) 365
FatI CATG 7 cut(s) 10, 288, 367, 595, 835, 862, 944
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 249, 705, 756, 963
FokI GGATG 3 cut(s) 315, 596, 653
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 4 cut(s) 249, 705, 756, 963
FspBI CTAG 1 cut(s) 659
GluI GCNGC 4 cut(s) 249, 705, 756, 963
HaeIII GGCC 3 cut(s) 439, 513, 594
HapII CCGG 2 cut(s) 265, 815
Hin1II CATG 7 cut(s) 14, 292, 371, 599, 839, 866, 948
HincII GTYRAC 2 cut(s) 187, 787
HindII GTYRAC 2 cut(s) 187, 787
HinfI GANTC 3 cut(s) 443, 572, 920
HpaII CCGG 2 cut(s) 265, 815
HphI GGTGA 2 cut(s) 138, 149
Hpy166II GTNNAC 7 cut(s) 103, 112, 122, 142, 170, 187, 787
Hpy188I TCNGA 4 cut(s) 336, 448, 571, 989
Hpy188III TCNNGA 2 cut(s) 128, 211
Hpy8I GTNNAC 7 cut(s) 103, 112, 122, 142, 170, 187, 787
HpyAV CCTTC 3 cut(s) 67, 286, 500
HpyCH4III ACNGT 3 cut(s) 100, 799, 908
HpyF10VI GCNNNNNNNGC 5 cut(s) 617, 701, 752, 854, 863
HpyF3I CTNAG 3 cut(s) 6, 333, 691
Hsp92II CATG 7 cut(s) 14, 292, 371, 599, 839, 866, 948
Kzo9I GATC 3 cut(s) 344, 976, 984
LmnI GCTCC 1 cut(s) 545
LpnPI CCDG 8 cut(s) 83, 141, 278, 414, 453, 828, 885, 927
Lsp1109I GCAGC 4 cut(s) 235, 691, 742, 974
LweI GCATC 3 cut(s) 733, 757, 830
MaeI CTAG 1 cut(s) 659
MaeIII GTNAC 1 cut(s) 221
MalI GATC 3 cut(s) 346, 978, 986
MboI GATC 3 cut(s) 344, 976, 984
MboII GAAGA 2 cut(s) 58, 558
MfeI CAATTG 1 cut(s) 957
MflI RGATCY 2 cut(s) 344, 984
MhlI GDGCHC 3 cut(s) 550, 727, 737
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 9 cut(s) 205, 321, 432, 517, 540, 559, 699, 892, 957
MluNI TGGCCA 1 cut(s) 513
MnlI CCTC 8 cut(s) 130, 342, 486, 489, 622, 671, 674, 700
Mox20I TGGCCA 1 cut(s) 513
MscI TGGCCA 1 cut(s) 513
MseI TTAA 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 513
MspI CCGG 2 cut(s) 265, 815
MspR9I CCNGG 2 cut(s) 266, 815
MunI CAATTG 1 cut(s) 957
MwoI GCNNNNNNNGC 5 cut(s) 617, 701, 752, 854, 863
NciI CCSGG 2 cut(s) 266, 815
NcoI CCATGG 2 cut(s) 595, 944
NdeII GATC 3 cut(s) 344, 976, 984
NlaIII CATG 7 cut(s) 14, 292, 371, 599, 839, 866, 948
NlaIV GGNNCC 3 cut(s) 601, 687, 734
NspI RCATGY 1 cut(s) 14
PfeI GAWTC 3 cut(s) 443, 572, 920
PflMI CCANNNNNTGG 2 cut(s) 489, 868
PkrI GCNGC 4 cut(s) 250, 706, 757, 964
Psp124BI GAGCTC 1 cut(s) 550
PspN4I GGNNCC 3 cut(s) 601, 687, 734
PspPI GGNCC 3 cut(s) 100, 473, 911
PstI CTGCAG 3 cut(s) 202, 622, 901
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 2 cut(s) 344, 984
RsaI GTAC 4 cut(s) 113, 143, 670, 973
RsaNI GTAC 4 cut(s) 112, 142, 669, 972
SacI GAGCTC 1 cut(s) 550
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 4 cut(s) 249, 705, 756, 963
Sau3AI GATC 3 cut(s) 344, 976, 984
Sau96I GGNCC 3 cut(s) 100, 473, 911
ScaI AGTACT 1 cut(s) 973
ScrFI CCNGG 2 cut(s) 266, 815
SduI GDGCHC 3 cut(s) 550, 727, 737
SfaNI GCATC 3 cut(s) 733, 757, 830
SfcI CTRYAG 3 cut(s) 198, 618, 897
SinI GGWCC 3 cut(s) 100, 473, 911
Sse9I AATT 9 cut(s) 205, 321, 432, 517, 540, 559, 699, 892, 957
SspMI CTAG 1 cut(s) 659
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 264, 813
StyI CCWWGG 4 cut(s) 115, 163, 595, 944
TaaI ACNGT 3 cut(s) 100, 799, 908
TaqI TCGA 1 cut(s) 186
TasI AATT 9 cut(s) 205, 321, 432, 517, 540, 559, 699, 892, 957
TatI WGTACW 2 cut(s) 141, 971
TfiI GAWTC 3 cut(s) 443, 572, 920
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 4 cut(s) 244, 265, 397, 433
TseI GCWGC 4 cut(s) 248, 704, 755, 962
TspDTI ATGAA 5 cut(s) 102, 305, 552, 600, 792
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 4 cut(s) 244, 265, 397, 433
Van91I CCANNNNNTGG 2 cut(s) 489, 868
VpaK11BI GGWCC 3 cut(s) 100, 473, 911
XapI RAATTY 5 cut(s) 205, 321, 432, 517, 892
XceI RCATGY 1 cut(s) 14
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 659
ZrmI AGTACT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.