Subtilisin-like serine endopeptidase family protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1748 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 2 cut(s) | 95, 1588 |
| AccI | GTMKAC | 1 cut(s) | 405 |
| AciI | CCGC | 3 cut(s) | 330, 1032, 1701 |
| AclWI | GGATC | 5 cut(s) | 1127, 1140, 1286, 1360, 1637 |
| AcsI | RAATTY | 5 cut(s) | 252, 617, 726, 811, 1121 |
| AcuI | CTGAAG | 2 cut(s) | 1311, 1509 |
| AcvI | CACGTG | 1 cut(s) | 1718 |
| AdeI | CACNNNGTG | 1 cut(s) | 1042 |
| AfaI | GTAC | 4 cut(s) | 28, 55, 434, 961 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1416 |
| AgsI | TTSAA | 7 cut(s) | 155, 277, 337, 532, 622, 752, 906 |
| AhlI | ACTAGT | 1 cut(s) | 1315 |
| AjiI | CACGTC | 1 cut(s) | 1391 |
| AjnI | CCWGG | 2 cut(s) | 477, 558 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1687, 1719 |
| Alw21I | GWGCWC | 4 cut(s) | 140, 614, 844, 1522 |
| Alw26I | GTCTC | 3 cut(s) | 450, 601, 1576 |
| Alw44I | GTGCAC | 1 cut(s) | 1518 |
| AlwI | GGATC | 5 cut(s) | 1127, 1140, 1286, 1360, 1637 |
| Ama87I | CYCGRG | 2 cut(s) | 133, 1647 |
| AoxI | GGCC | 2 cut(s) | 257, 731 |
| ApaLI | GTGCAC | 1 cut(s) | 1518 |
| ApeKI | GCWGC | 8 cut(s) | 542, 911, 995, 1049, 1232, 1256, 1688, 1762 |
| ApoI | RAATTY | 5 cut(s) | 252, 617, 726, 811, 1121 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 272, 304, 746, 778 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 487, 1337 |
| AspS9I | GGNCC | 4 cut(s) | 15, 293, 1205, 1721 |
| AsuC2I | CCSGG | 1 cut(s) | 1685 |
| AsuHPI | GGTGA | 4 cut(s) | 64, 429, 440, 1565 |
| AvaI | CYCGRG | 2 cut(s) | 133, 1647 |
| AvaII | GGWCC | 4 cut(s) | 15, 293, 1205, 1721 |
| BaeGI | GKGCMC | 1 cut(s) | 1522 |
| BamHI | GGATCC | 1 cut(s) | 1132 |
| BanII | GRGCYC | 2 cut(s) | 844, 1798 |
| BbrPI | CACGTG | 1 cut(s) | 1718 |
| BbsI | GAAGAC | 3 cut(s) | 11, 121, 1808 |
| Bbv12I | GWGCWC | 4 cut(s) | 140, 614, 844, 1522 |
| BbvI | GCAGC | 8 cut(s) | 529, 923, 1007, 1036, 1244, 1268, 1675, 1749 |
| BccI | CCATC | 4 cut(s) | 448, 608, 1067, 1106 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1300, 1334 |
| BciT130I | CCWGG | 2 cut(s) | 479, 560 |
| BciVI | GTATCC | 1 cut(s) | 1167 |
| BclI | TGATCA | 1 cut(s) | 507 |
| BcnI | CCSGG | 1 cut(s) | 1685 |
| BcoDI | GTCTC | 3 cut(s) | 450, 601, 1576 |
| BcuI | ACTAGT | 1 cut(s) | 1315 |
| BfaI | CTAG | 5 cut(s) | 950, 1298, 1316, 1523, 1638 |
| BfmI | CTRYAG | 4 cut(s) | 85, 860, 1027, 1209 |
| BfuAI | ACCTGC | 2 cut(s) | 95, 1588 |
| BfuI | GTATCC | 1 cut(s) | 1167 |
| BglII | AGATCT | 3 cut(s) | 164, 638, 1108 |
| BmcAI | AGTACT | 1 cut(s) | 55 |
| Bme1390I | CCNGG | 3 cut(s) | 479, 560, 1685 |
| Bme18I | GGWCC | 4 cut(s) | 15, 293, 1205, 1721 |
| BmeT110I | CYCGRG | 2 cut(s) | 133, 1647 |
| BmgBI | CACGTC | 1 cut(s) | 1391 |
| BmgT120I | GGNCC | 4 cut(s) | 15, 293, 1205, 1721 |
| BmiI | GGNNCC | 2 cut(s) | 774, 1134 |
| BmrFI | CCNGG | 3 cut(s) | 479, 560, 1685 |
| BmsI | GCATC | 3 cut(s) | 1051, 1372, 1468 |
| BoxI | GACNNNNGTC | 1 cut(s) | 94 |
| BpiI | GAAGAC | 3 cut(s) | 11, 121, 1808 |
| BpuMI | CCSGG | 1 cut(s) | 1685 |
| BsaAI | YACGTR | 1 cut(s) | 1718 |
| BsaJI | CCNNGG | 5 cut(s) | 75, 454, 478, 1161, 1238 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1616, 1646 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1416 |
| Bse1I | ACTGG | 1 cut(s) | 1555 |
| Bse3DI | GCAATG | 4 cut(s) | 217, 805, 1065, 1204 |
| BseBI | CCWGG | 2 cut(s) | 479, 560 |
| BseDI | CCNNGG | 5 cut(s) | 75, 454, 478, 1161, 1238 |
| BseGI | GGATG | 1 cut(s) | 241 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1416 |
| BseMI | GCAATG | 4 cut(s) | 217, 805, 1065, 1204 |
| BseMII | CTCAG | 1 cut(s) | 180 |
| BseNI | ACTGG | 1 cut(s) | 1555 |
| BseRI | GAGGAG | 3 cut(s) | 288, 984, 987 |
| BseSI | GKGCMC | 1 cut(s) | 1522 |
| BseXI | GCAGC | 8 cut(s) | 529, 923, 1007, 1036, 1244, 1268, 1675, 1749 |
| BseYI | CCCAGC | 1 cut(s) | 1415 |
| BsgI | GTGCAG | 2 cut(s) | 1251, 1674 |
| BshFI | GGCC | 2 cut(s) | 259, 733 |
| BsiHKAI | GWGCWC | 4 cut(s) | 140, 614, 844, 1522 |
| BsiHKCI | CYCGRG | 2 cut(s) | 133, 1647 |
| BsiSI | CCGG | 1 cut(s) | 1685 |
| BslFI | GGGAC | 2 cut(s) | 185, 1336 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1416 |
| BsmAI | GTCTC | 3 cut(s) | 450, 601, 1576 |
| BsmBI | CGTCTC | 1 cut(s) | 450 |
| BsmFI | GGGAC | 2 cut(s) | 185, 1336 |
| BsmI | GAATGC | 2 cut(s) | 1403, 1481 |
| BsnI | GGCC | 2 cut(s) | 259, 733 |
| BsoBI | CYCGRG | 2 cut(s) | 133, 1647 |
| Bsp1286I | GDGCHC | 5 cut(s) | 140, 614, 844, 1522, 1798 |
| Bsp1407I | TGTACA | 1 cut(s) | 26 |
| Bsp19I | CCATGG | 2 cut(s) | 75, 1161 |
| BspACI | CCGC | 3 cut(s) | 330, 1032, 1701 |
| BspANI | GGCC | 2 cut(s) | 259, 733 |
| BspCNI | CTCAG | 1 cut(s) | 181 |
| BspHI | TCATGA | 1 cut(s) | 1393 |
| BspLI | GGNNCC | 2 cut(s) | 774, 1134 |
| BspMAI | CTGCAG | 2 cut(s) | 864, 1213 |
| BspMI | ACCTGC | 2 cut(s) | 95, 1588 |
| BspPI | GGATC | 5 cut(s) | 1127, 1140, 1286, 1360, 1637 |
| BsrDI | GCAATG | 4 cut(s) | 217, 805, 1065, 1204 |
| BsrGI | TGTACA | 1 cut(s) | 26 |
| BsrI | ACTGG | 1 cut(s) | 1555 |
| BssECI | CCNNGG | 5 cut(s) | 75, 454, 478, 1161, 1238 |
| BssNAI | GTATAC | 1 cut(s) | 406 |
| BssT1I | CCWWGG | 4 cut(s) | 75, 454, 1161, 1238 |
| Bst1107I | GTATAC | 1 cut(s) | 406 |
| Bst2UI | CCWGG | 2 cut(s) | 479, 560 |
| Bst4CI | ACNGT | 6 cut(s) | 93, 430, 1222, 1252, 1445, 1776 |
| Bst6I | CTCTTC | 2 cut(s) | 478, 1717 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 992, 1697 |
| BstAUI | TGTACA | 1 cut(s) | 26 |
| BstBAI | YACGTR | 1 cut(s) | 1718 |
| BstC8I | GCNNGC | 3 cut(s) | 796, 1195, 1213 |
| BstDEI | CTNAG | 3 cut(s) | 189, 303, 409 |
| BstDSI | CCRYGG | 2 cut(s) | 75, 1161 |
| BstF5I | GGATG | 1 cut(s) | 241 |
| BstMAI | GTCTC | 3 cut(s) | 450, 601, 1576 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 366, 804, 908, 992, 1511, 1697 |
| BstNI | CCWGG | 2 cut(s) | 479, 560 |
| BstNSI | RCATGY | 1 cut(s) | 1816 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 94 |
| BstSCI | CCNGG | 3 cut(s) | 477, 558, 1683 |
| BstSFI | CTRYAG | 4 cut(s) | 85, 860, 1027, 1209 |
| BstSLI | GKGCMC | 1 cut(s) | 1522 |
| BstV1I | GCAGC | 8 cut(s) | 529, 923, 1007, 1036, 1244, 1268, 1675, 1749 |
| BstV2I | GAAGAC | 3 cut(s) | 11, 121, 1808 |
| BstX2I | RGATCY | 5 cut(s) | 164, 638, 1108, 1132, 1642 |
| BstYI | RGATCY | 5 cut(s) | 164, 638, 1108, 1132, 1642 |
| BstZ17I | GTATAC | 1 cut(s) | 406 |
| BsuI | GTATCC | 1 cut(s) | 1167 |
| BsuRI | GGCC | 2 cut(s) | 259, 733 |
| BtgI | CCRYGG | 2 cut(s) | 75, 1161 |
| BtrI | CACGTC | 1 cut(s) | 1391 |
| BtsCI | GGATG | 1 cut(s) | 241 |
| BtsI | GCAGTG | 2 cut(s) | 857, 897 |
| BtsIMutI | CAGTG | 5 cut(s) | 186, 857, 897, 1335, 1450 |
| BveI | ACCTGC | 2 cut(s) | 95, 1588 |
| Cac8I | GCNNGC | 3 cut(s) | 796, 1195, 1213 |
| CciI | TCATGA | 1 cut(s) | 1393 |
| Cfr13I | GGNCC | 4 cut(s) | 15, 293, 1205, 1721 |
| Csp6I | GTAC | 4 cut(s) | 27, 54, 433, 960 |
| CviQI | GTAC | 4 cut(s) | 27, 54, 433, 960 |
| DdeI | CTNAG | 3 cut(s) | 189, 303, 409 |
| DraI | TTTAAA | 1 cut(s) | 1536 |
| DraIII | CACNNNGTG | 1 cut(s) | 1042 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 13 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 13 |
| Eam1104I | CTCTTC | 2 cut(s) | 478, 1717 |
| EarI | CTCTTC | 2 cut(s) | 478, 1717 |
| Ecl136II | GAGCTC | 1 cut(s) | 842 |
| Eco130I | CCWWGG | 4 cut(s) | 75, 454, 1161, 1238 |
| Eco24I | GRGCYC | 2 cut(s) | 844, 1798 |
| Eco47I | GGWCC | 4 cut(s) | 15, 293, 1205, 1721 |
| Eco53kI | GAGCTC | 1 cut(s) | 842 |
| Eco57I | CTGAAG | 2 cut(s) | 1311, 1509 |
| Eco72I | CACGTG | 1 cut(s) | 1718 |
| Eco88I | CYCGRG | 2 cut(s) | 133, 1647 |
| EcoICRI | GAGCTC | 1 cut(s) | 842 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1205 |
| EcoRII | CCWGG | 2 cut(s) | 477, 558 |
| EcoT14I | CCWWGG | 4 cut(s) | 75, 454, 1161, 1238 |
| EcoT22I | ATGCAT | 1 cut(s) | 1483 |
| EcoT38I | GRGCYC | 2 cut(s) | 844, 1798 |
| ErhI | CCWWGG | 4 cut(s) | 75, 454, 1161, 1238 |
| Esp3I | CGTCTC | 1 cut(s) | 450 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 151, 183, 1422, 1454 |
| FaqI | GGGAC | 2 cut(s) | 185, 1336 |
| FbaI | TGATCA | 1 cut(s) | 507 |
| FblI | GTMKAC | 1 cut(s) | 405 |
| FokI | GGATG | 1 cut(s) | 248 |
| FriOI | GRGCYC | 2 cut(s) | 844, 1798 |
| FspBI | CTAG | 5 cut(s) | 950, 1298, 1316, 1523, 1638 |
| GsaI | CCCAGC | 1 cut(s) | 1419 |
| HaeIII | GGCC | 2 cut(s) | 259, 733 |
| HapII | CCGG | 1 cut(s) | 1685 |
| HincII | GTYRAC | 2 cut(s) | 580, 1081 |
| HindII | GTYRAC | 2 cut(s) | 580, 1081 |
| HindIII | AAGCTT | 1 cut(s) | 271 |
| HinfI | GANTC | 8 cut(s) | 245, 263, 657, 737, 1019, 1406, 1448, 1726 |
| HpaI | GTTAAC | 1 cut(s) | 580 |
| HpaII | CCGG | 1 cut(s) | 1685 |
| HphI | GGTGA | 4 cut(s) | 64, 429, 440, 1565 |
| Hpy166II | GTNNAC | 9 cut(s) | 27, 397, 406, 433, 580, 1081, 1333, 1520, 1721 |
| Hpy188III | TCNNGA | 6 cut(s) | 133, 249, 607, 752, 1321, 1394 |
| Hpy8I | GTNNAC | 9 cut(s) | 27, 397, 406, 433, 580, 1081, 1333, 1520, 1721 |
| Hpy99I | CGWCG | 1 cut(s) | 1278 |
| HpyAV | CCTTC | 6 cut(s) | 106, 385, 580, 922, 1196, 1717 |
| HpyCH4III | ACNGT | 6 cut(s) | 93, 430, 1222, 1252, 1445, 1776 |
| HpyCH4IV | ACGT | 2 cut(s) | 1390, 1717 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 366, 804, 908, 992, 1511, 1697 |
| HpyF3I | CTNAG | 3 cut(s) | 189, 303, 409 |
| HpySE526I | ACGT | 2 cut(s) | 1390, 1717 |
| Ksp22I | TGATCA | 1 cut(s) | 507 |
| KspAI | GTTAAC | 1 cut(s) | 580 |
| LmnI | GCTCC | 2 cut(s) | 778, 839 |
| Lsp1109I | GCAGC | 8 cut(s) | 529, 923, 1007, 1036, 1244, 1268, 1675, 1749 |
| LweI | GCATC | 3 cut(s) | 1051, 1372, 1468 |
| MaeI | CTAG | 5 cut(s) | 950, 1298, 1316, 1523, 1638 |
| MaeII | ACGT | 2 cut(s) | 1390, 1717 |
| MaeIII | GTNAC | 2 cut(s) | 35, 414 |
| MfeI | CAATTG | 2 cut(s) | 1143, 1695 |
| MflI | RGATCY | 5 cut(s) | 164, 638, 1108, 1132, 1642 |
| MhlI | GDGCHC | 5 cut(s) | 140, 614, 844, 1522, 1798 |
| MlyI | GAGTC | 2 cut(s) | 239, 1400 |
| MmeI | TCCRAC | 2 cut(s) | 1209, 1256 |
| Mph1103I | ATGCAT | 1 cut(s) | 1483 |
| MroXI | GAANNNNTTC | 2 cut(s) | 487, 1337 |
| MseI | TTAA | 6 cut(s) | 579, 1346, 1455, 1535, 1592, 1661 |
| MslI | CAYNNNNRTG | 1 cut(s) | 74 |
| MspI | CCGG | 1 cut(s) | 1685 |
| MspR9I | CCNGG | 3 cut(s) | 479, 560, 1685 |
| MunI | CAATTG | 2 cut(s) | 1143, 1695 |
| Mva1269I | GAATGC | 2 cut(s) | 1403, 1481 |
| MvaI | CCWGG | 2 cut(s) | 479, 560 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 366, 804, 908, 992, 1511, 1697 |
| NciI | CCSGG | 1 cut(s) | 1685 |
| NcoI | CCATGG | 2 cut(s) | 75, 1161 |
| NlaIV | GGNNCC | 2 cut(s) | 774, 1134 |
| NmuCI | GTSAC | 2 cut(s) | 35, 414 |
| NsiI | ATGCAT | 1 cut(s) | 1483 |
| NspI | RCATGY | 1 cut(s) | 1816 |
| PaeR7I | CTCGAG | 1 cut(s) | 1647 |
| PagI | TCATGA | 1 cut(s) | 1393 |
| PctI | GAATGC | 2 cut(s) | 1403, 1481 |
| PdmI | GAANNNNTTC | 2 cut(s) | 487, 1337 |
| PfeI | GAWTC | 6 cut(s) | 263, 657, 737, 1019, 1448, 1726 |
| PleI | GAGTC | 2 cut(s) | 239, 1400 |
| PmaCI | CACGTG | 1 cut(s) | 1718 |
| PmlI | CACGTG | 1 cut(s) | 1718 |
| PpsI | GAGTC | 2 cut(s) | 239, 1400 |
| Ppu21I | YACGTR | 1 cut(s) | 1718 |
| PpuMI | RGGWCCY | 1 cut(s) | 1205 |
| PshAI | GACNNNNGTC | 1 cut(s) | 94 |
| PsiI | TTATAA | 1 cut(s) | 1748 |
| Psp124BI | GAGCTC | 1 cut(s) | 844 |
| Psp5II | RGGWCCY | 1 cut(s) | 1205 |
| Psp6I | CCWGG | 2 cut(s) | 477, 558 |
| PspCI | CACGTG | 1 cut(s) | 1718 |
| PspFI | CCCAGC | 1 cut(s) | 1415 |
| PspGI | CCWGG | 2 cut(s) | 477, 558 |
| PspN4I | GGNNCC | 2 cut(s) | 774, 1134 |
| PspPI | GGNCC | 4 cut(s) | 15, 293, 1205, 1721 |
| PspPPI | RGGWCCY | 1 cut(s) | 1205 |
| PspXI | VCTCGAGB | 1 cut(s) | 1647 |
| PstI | CTGCAG | 2 cut(s) | 864, 1213 |
| PsuI | RGATCY | 5 cut(s) | 164, 638, 1108, 1132, 1642 |
| RsaI | GTAC | 4 cut(s) | 28, 55, 434, 961 |
| RsaNI | GTAC | 4 cut(s) | 27, 54, 433, 960 |
| RseI | CAYNNNNRTG | 1 cut(s) | 74 |
| SacI | GAGCTC | 1 cut(s) | 844 |
| SaqAI | TTAA | 6 cut(s) | 579, 1346, 1455, 1535, 1592, 1661 |
| Sau96I | GGNCC | 4 cut(s) | 15, 293, 1205, 1721 |
| SbfI | CCTGCAGG | 1 cut(s) | 1213 |
| ScaI | AGTACT | 1 cut(s) | 55 |
| SchI | GAGTC | 2 cut(s) | 239, 1400 |
| ScrFI | CCNGG | 3 cut(s) | 479, 560, 1685 |
| SdaI | CCTGCAGG | 1 cut(s) | 1213 |
| SduI | GDGCHC | 5 cut(s) | 140, 614, 844, 1522, 1798 |
| SfaNI | GCATC | 3 cut(s) | 1051, 1372, 1468 |
| SfcI | CTRYAG | 4 cut(s) | 85, 860, 1027, 1209 |
| Sfr274I | CTCGAG | 1 cut(s) | 1647 |
| SinI | GGWCC | 4 cut(s) | 15, 293, 1205, 1721 |
| SlaI | CTCGAG | 1 cut(s) | 1647 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 74 |
| SmlI | CTYRAG | 1 cut(s) | 1647 |
| SmoI | CTYRAG | 1 cut(s) | 1647 |
| SpeI | ACTAGT | 1 cut(s) | 1315 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1213 |
| SsiI | CCGC | 3 cut(s) | 330, 1032, 1701 |
| SspMI | CTAG | 5 cut(s) | 950, 1298, 1316, 1523, 1638 |
| SstI | GAGCTC | 1 cut(s) | 844 |
| StyD4I | CCNGG | 3 cut(s) | 477, 558, 1683 |
| StyI | CCWWGG | 4 cut(s) | 75, 454, 1161, 1238 |
| TaaI | ACNGT | 6 cut(s) | 93, 430, 1222, 1252, 1445, 1776 |
| TaiI | ACGT | 2 cut(s) | 1393, 1720 |
| TaqI | TCGA | 5 cut(s) | 690, 1056, 1114, 1648, 1771 |
| TaqII | GACCGA | 1 cut(s) | 1738 |
| TatI | WGTACW | 3 cut(s) | 26, 53, 432 |
| TauI | GCSGC | 2 cut(s) | 333, 1703 |
| TfiI | GAWTC | 6 cut(s) | 263, 657, 737, 1019, 1448, 1726 |
| Tru1I | TTAA | 6 cut(s) | 579, 1346, 1455, 1535, 1592, 1661 |
| Tru9I | TTAA | 6 cut(s) | 579, 1346, 1455, 1535, 1592, 1661 |
| TscAI | CASTG | 5 cut(s) | 193, 864, 904, 1335, 1450 |
| TseFI | GTSAC | 2 cut(s) | 35, 414 |
| TseI | GCWGC | 8 cut(s) | 542, 911, 995, 1049, 1232, 1256, 1688, 1762 |
| Tsp45I | GTSAC | 2 cut(s) | 35, 414 |
| TspGWI | ACGGA | 2 cut(s) | 960, 1560 |
| TspRI | CASTG | 5 cut(s) | 193, 864, 904, 1335, 1450 |
| VneI | GTGCAC | 1 cut(s) | 1518 |
| VpaK11BI | GGWCC | 4 cut(s) | 15, 293, 1205, 1721 |
| XapI | RAATTY | 5 cut(s) | 252, 617, 726, 811, 1121 |
| XceI | RCATGY | 1 cut(s) | 1816 |
| XhoI | CTCGAG | 1 cut(s) | 1647 |
| XmiI | GTMKAC | 1 cut(s) | 405 |
| XmnI | GAANNNNTTC | 2 cut(s) | 487, 1337 |
| XspI | CTAG | 5 cut(s) | 950, 1298, 1316, 1523, 1638 |
| ZrmI | AGTACT | 1 cut(s) | 55 |
| Zsp2I | ATGCAT | 1 cut(s) | 1483 |