MD02G1289100.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000257
Physical Location & Seq
Forward (+)
34538327 .. 34540508
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1289100.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGGCAAAGCACGGAGATCTCCTGGTTTCTTATGCTTTGTCCATTATTTTCATACTCCTCGTTAGCTTACCGTGTGAAGCCGTCAATCATGGAGATAGAAAGACATATATCGTATACTTAGGATCACTTCCTAAGGATGATGTGTTTTCGCCATTGTCTCATCACATTGGTATTCTCGAAAGGGTTGCCGAGAGTAGCTCTGCTTCGAACCTCTTGGTAAGAAGCTACAAAAGAAGCTTCAACGGATTCGCTGCAAAGCTCACTGACCGAGAAAGAGAAAGGGTTGCTAAGATGAAGGAAGTTAGCAATGTTATTGTTGGTGTCATCGATACTGGGATATGGCCTGAATCGGAAAGTTTTAGCGATGAAGGTTTTGGTCCAGCTCCAAGGAAGTGGGAAGGAGTTTGTGAAGGAGGCAAAAATTTCACTTGCAACAACAAGATCATTGGAGCTCGGTTTTACGTTGGTTCCTCAGCTAGAGATGATGTTGGTCATGGAACTCATACTGCCTCAACCGCAGCAGGAAATCCCGTAAGGGGTGTGAGCTTTTATGGACTGGCGCACGGAACTGCAACAGGAGGCGTTCCCTCCGCGAGAATTGCTACATATAAAGTTTGCGGTTCTAAGGAGTGCGCTACAGACAAGATATTGGCTGCTTTTGATGATGCTATTGCTGATGGAGTTGACATCATCACAATTTCAATTGGAAGCACAAGTGCAGACCCTTTCGATCGGGATGCAATAGCAATCGGTGCTTTCCATGCACTGGAGAAAGGGATACTAACCTCGCAGTCTGCAGGCAATGGCGGCCCTTATGAGGGTTCTGTATCAAGTGTAGCACCATGGATTCTTACGGTTGCAGCAAGTAGCATAGATCGTCGCATCATTGACAAGGTGGTTCTTGGAAACCGAAGGACGCTTGTTGGGAATTCTGCAAAGATGCGTCAAGTGAATGTGGAGAGTGATGCAGGTTGCCTAGATCCTAATTTAGTTAAGGGAAAGATTGTGGTATGTCGTTGGGCTTTTGATAGTATCGAGGTTCGTCGAGTCGGCGCAATAGGTGCACTAAGTGAGAGTATCATCCTTGATGATTCTGGCATTGTCCCACTACCTGCAGCAAGTTTTAGCAGCAAAGATTTTGACCTTGTCAAGTCCTACGTCAACTCCACAGGCAAACCTCGAGCGAAACTACCAAAAAGTGAAGTAATAAAAGATCTTGCTGCACCTACTGTTGCTTCCTTCTCTTCGCGGGGGCCTAATTTTATTTTACCTGAAATTATCAAGCCGGACATAACTGCCCCGGGGGTTGATATTTTGGCTGCATATTCACCTGTTGCTCCTCTCACAGAGAGTGTTGAAGACCGGAGGCATGTGAAATACAGTTTCCTATCTGGAACCTCCATGTCTTGCCCGCATGCTGCTGGTGTCGCTGCGTATATCAAGACATTCCACCCCAACTGGTCTCCAGCTGCCATCAAATCATCTATCATGACTACGGCTGGGCCTGTAAATAGCACCGACGGTTCCTACTGGCCCGGTGTATTTTCATATGGTTCTGGGCATATCAATCCTCTAAAAGCTTTAAATCCGGGCCTTGTGTACGAAACTTCTAAGGATGATTACATTAAGTTGCTCTGTTCGATCTTGGATGAGTCCAAAGTTAGACTTATTTCCGGAGACAAACGCCCTTGCCCTGCAAGGACTGGCAAAAAATGGGCCAAGGATCTCAACTACCCTTCACTGGCTGGTAAAGTTGTAACACAGTCTTCGTTTTCGATTCAGTTTCAAAGAACAGTTAAAAATGTTGGCCATGCAAACTCCACTTACAAGGCAAAGACCATCTCGAAGCCGGGAGTTAACATCAAGGTGGTGCCTCAAGTTCTTTCCTTCAAGTCTTTGAATGAGAAAAAGACCTTCAATGTGACAGTTTCCGGAAGAGATATACCATCGCGTACGTATGTATCTGCGTCGTTGGTGTGGACGGATGGTACTCACAGCGTTCGAAGTCCAATTCTTCTAAGACTCGTCCTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

679

Amino Acids

72.41

Weight (kDa)

9.2

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 105 2.1e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 102 - 504 3.1e-45 Subtilase family
fn3_6 PF17766 575 - 672 3.1e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 959, 1120
AccB1I GGYRCC 1 cut(s) 1870
AccB7I CCANNNNNTGG 1 cut(s) 766
AccI GTMKAC 1 cut(s) 114
AccII CGCG 3 cut(s) 593, 1249, 1951
AccIII TCCGGA 2 cut(s) 1673, 1931
AciI CCGC 6 cut(s) 516, 591, 618, 807, 1249, 1412
AclWI GGATC 3 cut(s) 130, 974, 1731
AcoI YGGCCR 1 cut(s) 1807
AcsI RAATTY 2 cut(s) 421, 928
AdeI CACNNNGTG 1 cut(s) 1070
AfaI GTAC 3 cut(s) 1601, 1954, 1990
AfiI CCNNNNNNNGG 5 cut(s) 536, 766, 817, 818, 1741
AgsI TTSAA 7 cut(s) 241, 702, 1358, 1787, 1891, 1900, 1918
AjnI CCWGG 1 cut(s) 21
Alw21I GWGCWC 2 cut(s) 454, 1066
Alw26I GTCTC 3 cut(s) 162, 1467, 1671
Alw44I GTGCAC 1 cut(s) 1062
AlwI GGATC 3 cut(s) 130, 974, 1731
Ama87I CYCGRG 2 cut(s) 1179, 1300
Aor13HI TCCGGA 2 cut(s) 1673, 1931
AoxI GGCC 8 cut(s) 341, 808, 1253, 1502, 1532, 1591, 1716, 1807
ApaLI GTGCAC 1 cut(s) 1062
ApoI RAATTY 2 cut(s) 421, 928
AspLEI GCGC 3 cut(s) 562, 635, 1055
AspS9I GGNCC 7 cut(s) 377, 809, 1253, 1502, 1533, 1591, 1716
AsuC2I CCSGG 5 cut(s) 1301, 1302, 1536, 1590, 1851
AsuHPI GGTGA 1 cut(s) 1320
AsuII TTCGAA 2 cut(s) 206, 2002
AvaI CYCGRG 2 cut(s) 1179, 1300
AvaII GGWCC 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 1066
BaeI ACNNNNGTAYC 2 cut(s) 1944, 1977
BalI TGGCCA 1 cut(s) 1809
BanI GGYRCC 1 cut(s) 1870
BanII GRGCYC 1 cut(s) 454
BarI GAAGNNNNNNTAC 2 cut(s) 187, 219
BbsI GAAGAC 2 cut(s) 1365, 1758
Bbv12I GWGCWC 2 cut(s) 454, 1066
BbvCI CCTCAGC 1 cut(s) 472
BccI CCATC 5 cut(s) 671, 1481, 1847, 1954, 1979
BceAI ACGGC 2 cut(s) 65, 1512
BcgI CGANNNNNNTGC 4 cut(s) 167, 201, 719, 753
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 1 cut(s) 771
BcnI CCSGG 5 cut(s) 1301, 1302, 1536, 1590, 1851
BcoDI GTCTC 3 cut(s) 162, 1467, 1671
BfaI CTAG 2 cut(s) 477, 977
BfmI CTRYAG 3 cut(s) 636, 795, 1113
BfuAI ACCTGC 2 cut(s) 959, 1120
BfuI GTATCC 1 cut(s) 771
BglII AGATCT 2 cut(s) 16, 1213
Bme1390I CCNGG 6 cut(s) 23, 1301, 1302, 1536, 1590, 1851
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 2 cut(s) 1179, 1300
BmgT120I GGNCC 7 cut(s) 377, 809, 1253, 1502, 1533, 1591, 1716
BmiI GGNNCC 5 cut(s) 469, 1254, 1396, 1525, 1872
BmrFI CCNGG 6 cut(s) 23, 1301, 1302, 1536, 1590, 1851
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 5 cut(s) 655, 727, 891, 930, 955
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 1365, 1758
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 2 cut(s) 788, 1449
Bpu10I CCTNAGC 1 cut(s) 472
Bpu14I TTCGAA 2 cut(s) 206, 2002
BpuEI CTTGAG 1 cut(s) 1860
BpuMI CCSGG 5 cut(s) 1301, 1302, 1536, 1590, 1851
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 1956
BsaI GGTCTC 1 cut(s) 1467
BsaJI CCNNGG 6 cut(s) 386, 842, 1299, 1300, 1301, 1719
BsaWI WCCGGW 3 cut(s) 1362, 1673, 1931
BsaXI ACNNNNNCTCC 2 cut(s) 1148, 1178
Bsc4I CCNNNNNNNGG 5 cut(s) 536, 766, 817, 818, 1741
Bse1I ACTGG 7 cut(s) 337, 561, 771, 1463, 1535, 1708, 1746
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 2 cut(s) 313, 808
BseAI TCCGGA 2 cut(s) 1673, 1931
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 6 cut(s) 386, 842, 1299, 1300, 1301, 1719
BseGI GGATG 6 cut(s) 142, 742, 1080, 1621, 1654, 1990
BseLI CCNNNNNNNGG 5 cut(s) 536, 766, 817, 818, 1741
BseMI GCAATG 2 cut(s) 313, 808
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 7 cut(s) 337, 561, 771, 1463, 1535, 1708, 1746
BseRI GAGGAG 2 cut(s) 47, 1329
BseSI GKGCMC 1 cut(s) 1066
BseYI CCCAGC 1 cut(s) 1499
BsgI GTGCAG 2 cut(s) 738, 1206
Bsh1236I CGCG 3 cut(s) 593, 1249, 1951
Bsh1285I CGRYCG 1 cut(s) 733
BshFI GGCC 8 cut(s) 343, 810, 1255, 1504, 1534, 1593, 1718, 1809
BshNI GGYRCC 1 cut(s) 1870
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 733
BsiHKAI GWGCWC 2 cut(s) 454, 1066
BsiHKCI CYCGRG 2 cut(s) 1179, 1300
BsiSI CCGG 8 cut(s) 1286, 1301, 1363, 1536, 1589, 1674, 1850, 1932
BsiWI CGTACG 1 cut(s) 1952
BslFI GGGAC 1 cut(s) 1088
BslI CCNNNNNNNGG 5 cut(s) 536, 766, 817, 818, 1741
BsmAI GTCTC 3 cut(s) 162, 1467, 1671
BsmFI GGGAC 1 cut(s) 1088
BsnI GGCC 8 cut(s) 343, 810, 1255, 1504, 1534, 1593, 1718, 1809
Bso31I GGTCTC 1 cut(s) 1467
BsoBI CYCGRG 2 cut(s) 1179, 1300
Bsp119I TTCGAA 2 cut(s) 206, 2002
Bsp1286I GDGCHC 2 cut(s) 454, 1066
Bsp13I TCCGGA 2 cut(s) 1673, 1931
Bsp143I GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
Bsp19I CCATGG 1 cut(s) 842
BspACI CCGC 6 cut(s) 516, 591, 618, 807, 1249, 1412
BspANI GGCC 8 cut(s) 343, 810, 1255, 1504, 1534, 1593, 1718, 1809
BspCNI CTCAG 1 cut(s) 485
BspDI ATCGAT 1 cut(s) 327
BspEI TCCGGA 2 cut(s) 1673, 1931
BspFNI CGCG 3 cut(s) 593, 1249, 1951
BspHI TCATGA 1 cut(s) 1488
BspLI GGNNCC 5 cut(s) 469, 1254, 1396, 1525, 1872
BspMAI CTGCAG 2 cut(s) 799, 1117
BspMI ACCTGC 2 cut(s) 959, 1120
BspPI GGATC 3 cut(s) 130, 974, 1731
BspT104I TTCGAA 2 cut(s) 206, 2002
BspT107I GGYRCC 1 cut(s) 1870
BspTNI GGTCTC 1 cut(s) 1467
BsrDI GCAATG 2 cut(s) 313, 808
BsrI ACTGG 7 cut(s) 337, 561, 771, 1463, 1535, 1708, 1746
BssECI CCNNGG 6 cut(s) 386, 842, 1299, 1300, 1301, 1719
BssMI GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
BssNAI GTATAC 1 cut(s) 115
BssT1I CCWWGG 3 cut(s) 386, 842, 1719
Bst1107I GTATAC 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 8 cut(s) 72, 856, 1231, 1382, 1523, 1764, 1795, 1927
Bst6I CTCTTC 2 cut(s) 1249, 1930
BstAPI GCANNNNNTGC 2 cut(s) 752, 1061
BstBAI YACGTR 1 cut(s) 1956
BstBI TTCGAA 2 cut(s) 206, 2002
BstC8I GCNNGC 3 cut(s) 799, 1412, 1416
BstDEI CTNAG 8 cut(s) 118, 132, 288, 472, 624, 1067, 1611, 2018
BstDSI CCRYGG 1 cut(s) 842
BstF5I GGATG 6 cut(s) 142, 742, 1080, 1621, 1654, 1990
BstFNI CGCG 3 cut(s) 593, 1249, 1951
BstHHI GCGC 3 cut(s) 562, 635, 1055
BstKTI GATC 9 cut(s) 19, 125, 444, 733, 877, 982, 1216, 1644, 1726
BstMAI GTCTC 3 cut(s) 162, 1467, 1671
BstMBI GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
BstMCI CGRYCG 1 cut(s) 733
BstMWI GCNNNNNNNGC 7 cut(s) 515, 599, 752, 761, 807, 1061, 1427
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 2 cut(s) 1373, 1418
BstSCI CCNGG 6 cut(s) 21, 1299, 1300, 1534, 1588, 1849
BstSFI CTRYAG 3 cut(s) 636, 795, 1113
BstSLI GKGCMC 1 cut(s) 1066
BstSNI TACGTA 1 cut(s) 1956
BstUI CGCG 3 cut(s) 593, 1249, 1951
BstV2I GAAGAC 2 cut(s) 1365, 1758
BstX2I RGATCY 4 cut(s) 16, 979, 1213, 1723
BstXI CCANNNNNNTGG 1 cut(s) 393
BstYI RGATCY 4 cut(s) 16, 979, 1213, 1723
BstZ17I GTATAC 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 327
Bsu36I CCTNAGG 1 cut(s) 132
BsuI GTATCC 1 cut(s) 771
BsuRI GGCC 8 cut(s) 343, 810, 1255, 1504, 1534, 1593, 1718, 1809
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 842
BtgZI GCGATG 2 cut(s) 378, 1932
BtsCI GGATG 6 cut(s) 142, 742, 1080, 1621, 1654, 1990
BtsIMutI CAGTG 3 cut(s) 261, 764, 1739
BveI ACCTGC 2 cut(s) 959, 1120
Cac8I GCNNGC 3 cut(s) 799, 1412, 1416
CciI TCATGA 1 cut(s) 1488
CfoI GCGC 3 cut(s) 562, 635, 1055
Cfr13I GGNCC 7 cut(s) 377, 809, 1253, 1502, 1533, 1591, 1716
Cfr9I CCCGGG 1 cut(s) 1300
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 3 cut(s) 925, 932, 1956
Csp6I GTAC 3 cut(s) 1600, 1953, 1989
CviAII CATG 9 cut(s) 89, 494, 761, 843, 1370, 1402, 1415, 1489, 1811
CviQI GTAC 3 cut(s) 1600, 1953, 1989
DdeI CTNAG 8 cut(s) 118, 132, 288, 472, 624, 1067, 1611, 2018
DpnI GATC 9 cut(s) 18, 124, 443, 732, 876, 981, 1215, 1643, 1725
DpnII GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
DraI TTTAAA 1 cut(s) 1584
DraIII CACNNNGTG 1 cut(s) 1070
EaeI YGGCCR 1 cut(s) 1807
Eam1104I CTCTTC 2 cut(s) 1249, 1930
EarI CTCTTC 2 cut(s) 1249, 1930
Ecl136II GAGCTC 1 cut(s) 452
Eco105I TACGTA 1 cut(s) 1956
Eco130I CCWWGG 3 cut(s) 386, 842, 1719
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 1467
Eco47I GGWCC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 452
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 1179, 1300
EcoICRI GAGCTC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 1253
EcoRI GAATTC 1 cut(s) 928
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 3 cut(s) 386, 842, 1719
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 3 cut(s) 386, 842, 1719
FaeI CATG 9 cut(s) 92, 497, 764, 846, 1373, 1405, 1418, 1492, 1814
FalI AAGNNNNNCTT 2 cut(s) 1650, 1682
FaqI GGGAC 1 cut(s) 1088
FatI CATG 9 cut(s) 88, 493, 760, 842, 1369, 1401, 1414, 1488, 1810
FauI CCCGC 2 cut(s) 1242, 1419
FauNDI CATATG 1 cut(s) 1549
FblI GTMKAC 1 cut(s) 114
FokI GGATG 6 cut(s) 149, 749, 1067, 1628, 1661, 1997
FriOI GRGCYC 1 cut(s) 454
FspBI CTAG 2 cut(s) 477, 977
GlaI GCGC 3 cut(s) 561, 634, 1054
GsaI CCCAGC 1 cut(s) 1503
GsuI CTGGAG 2 cut(s) 788, 1449
HaeIII GGCC 8 cut(s) 343, 810, 1255, 1504, 1534, 1593, 1718, 1809
HapII CCGG 8 cut(s) 1286, 1301, 1363, 1536, 1589, 1674, 1850, 1932
HgaI GACGC 3 cut(s) 925, 932, 1956
HhaI GCGC 3 cut(s) 562, 635, 1055
Hin1II CATG 9 cut(s) 92, 497, 764, 846, 1373, 1405, 1418, 1492, 1814
Hin6I GCGC 3 cut(s) 560, 633, 1053
HinP1I GCGC 3 cut(s) 560, 633, 1053
HincII GTYRAC 3 cut(s) 685, 1162, 1858
HindII GTYRAC 3 cut(s) 685, 1162, 1858
HindIII AAGCTT 2 cut(s) 235, 1578
HinfI GANTC 8 cut(s) 246, 347, 847, 1047, 1091, 1652, 1777, 2022
HpaI GTTAAC 1 cut(s) 1858
HpaII CCGG 8 cut(s) 1286, 1301, 1363, 1536, 1589, 1674, 1850, 1932
HphI GGTGA 1 cut(s) 1320
Hpy166II GTNNAC 7 cut(s) 115, 685, 1064, 1162, 1600, 1858, 1980
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 8 cut(s) 176, 734, 1392, 1441, 1489, 1674, 1843, 1932
Hpy8I GTNNAC 7 cut(s) 115, 685, 1064, 1162, 1600, 1858, 1980
Hpy99I CGWCG 4 cut(s) 882, 1047, 1523, 1972
HpyAV CCTTC 9 cut(s) 289, 362, 392, 404, 906, 1249, 1746, 1897, 1924
HpyCH4III ACNGT 8 cut(s) 72, 856, 1231, 1382, 1523, 1764, 1795, 1927
HpyCH4IV ACGT 3 cut(s) 462, 1158, 1955
HpyF10VI GCNNNNNNNGC 7 cut(s) 515, 599, 752, 761, 807, 1061, 1427
HpyF3I CTNAG 8 cut(s) 118, 132, 288, 472, 624, 1067, 1611, 2018
HpySE526I ACGT 3 cut(s) 462, 1158, 1955
Hsp92II CATG 9 cut(s) 92, 497, 764, 846, 1373, 1405, 1418, 1492, 1814
HspAI GCGC 3 cut(s) 560, 633, 1053
Kpn2I TCCGGA 2 cut(s) 1673, 1931
KspAI GTTAAC 1 cut(s) 1858
Kzo9I GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
LmnI GCTCC 3 cut(s) 388, 449, 1342
LweI GCATC 5 cut(s) 655, 727, 891, 930, 955
MaeI CTAG 2 cut(s) 477, 977
MaeII ACGT 3 cut(s) 462, 1158, 1955
MaeIII GTNAC 2 cut(s) 1756, 1921
MalI GATC 9 cut(s) 18, 124, 443, 732, 876, 981, 1215, 1643, 1725
MboI GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
MboII GAAGA 5 cut(s) 1236, 1370, 1758, 1947, 2006
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 4 cut(s) 16, 979, 1213, 1723
MhlI GDGCHC 2 cut(s) 454, 1066
MlsI TGGCCA 1 cut(s) 1809
MluCI AATT 9 cut(s) 421, 597, 696, 702, 928, 985, 1258, 1275, 2010
MluNI TGGCCA 1 cut(s) 1809
MlyI GAGTC 3 cut(s) 1056, 1661, 2016
Mox20I TGGCCA 1 cut(s) 1809
MroI TCCGGA 2 cut(s) 1673, 1931
MscI TGGCCA 1 cut(s) 1809
MseI TTAA 5 cut(s) 993, 1583, 1626, 1797, 1857
MslI CAYNNNNRTG 1 cut(s) 1865
Msp20I TGGCCA 1 cut(s) 1809
MspA1I CMGCKG 1 cut(s) 1469
MspI CCGG 8 cut(s) 1286, 1301, 1363, 1536, 1589, 1674, 1850, 1932
MspR9I CCNGG 6 cut(s) 23, 1301, 1302, 1536, 1590, 1851
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 3 cut(s) 593, 1249, 1951
MwoI GCNNNNNNNGC 7 cut(s) 515, 599, 752, 761, 807, 1061, 1427
NciI CCSGG 5 cut(s) 1301, 1302, 1536, 1590, 1851
NcoI CCATGG 1 cut(s) 842
NdeI CATATG 1 cut(s) 1549
NdeII GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
NlaIII CATG 9 cut(s) 92, 497, 764, 846, 1373, 1405, 1418, 1492, 1814
NlaIV GGNNCC 5 cut(s) 469, 1254, 1396, 1525, 1872
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 1921
NspI RCATGY 2 cut(s) 1373, 1418
NspV TTCGAA 2 cut(s) 206, 2002
PaeI GCATGC 1 cut(s) 1418
PaeR7I CTCGAG 1 cut(s) 1179
PagI TCATGA 1 cut(s) 1488
PfeI GAWTC 5 cut(s) 246, 347, 847, 1091, 1777
Pfl23II CGTACG 1 cut(s) 1952
PflFI GACNNNGTC 1 cut(s) 1145
PflMI CCANNNNNTGG 1 cut(s) 766
Ple19I CGATCG 1 cut(s) 733
PleI GAGTC 3 cut(s) 1055, 1660, 2016
PpsI GAGTC 3 cut(s) 1055, 1660, 2016
Ppu21I YACGTR 1 cut(s) 1956
Psp124BI GAGCTC 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 21
PspFI CCCAGC 1 cut(s) 1499
PspGI CCWGG 1 cut(s) 21
PspLI CGTACG 1 cut(s) 1952
PspN4I GGNNCC 5 cut(s) 469, 1254, 1396, 1525, 1872
PspPI GGNCC 7 cut(s) 377, 809, 1253, 1502, 1533, 1591, 1716
PspXI VCTCGAGB 1 cut(s) 1179
PstI CTGCAG 2 cut(s) 799, 1117
PsuI RGATCY 4 cut(s) 16, 979, 1213, 1723
PsyI GACNNNGTC 1 cut(s) 1145
PvuI CGATCG 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 1469
RsaI GTAC 3 cut(s) 1601, 1954, 1990
RsaNI GTAC 3 cut(s) 1600, 1953, 1989
RseI CAYNNNNRTG 1 cut(s) 1865
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 5 cut(s) 993, 1583, 1626, 1797, 1857
Sau3AI GATC 9 cut(s) 16, 122, 441, 730, 874, 979, 1213, 1641, 1723
Sau96I GGNCC 7 cut(s) 377, 809, 1253, 1502, 1533, 1591, 1716
SchI GAGTC 3 cut(s) 1056, 1661, 2016
ScrFI CCNGG 6 cut(s) 23, 1301, 1302, 1536, 1590, 1851
SduI GDGCHC 2 cut(s) 454, 1066
SfaNI GCATC 5 cut(s) 655, 727, 891, 930, 955
SfcI CTRYAG 3 cut(s) 636, 795, 1113
Sfr274I CTCGAG 1 cut(s) 1179
SfuI TTCGAA 2 cut(s) 206, 2002
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 1179
SmaI CCCGGG 1 cut(s) 1302
SmiMI CAYNNNNRTG 1 cut(s) 1865
SmlI CTYRAG 2 cut(s) 1179, 1875
SmoI CTYRAG 2 cut(s) 1179, 1875
SnaBI TACGTA 1 cut(s) 1956
SphI GCATGC 1 cut(s) 1418
Sse9I AATT 9 cut(s) 421, 597, 696, 702, 928, 985, 1258, 1275, 2010
SsiI CCGC 6 cut(s) 516, 591, 618, 807, 1249, 1412
SspMI CTAG 2 cut(s) 477, 977
SstI GAGCTC 1 cut(s) 454
StyD4I CCNGG 6 cut(s) 21, 1299, 1300, 1534, 1588, 1849
StyI CCWWGG 3 cut(s) 386, 842, 1719
TaaI ACNGT 8 cut(s) 72, 856, 1231, 1382, 1523, 1764, 1795, 1927
TaiI ACGT 3 cut(s) 465, 1161, 1958
TaqII GACCGA 1 cut(s) 282
TasI AATT 9 cut(s) 421, 597, 696, 702, 928, 985, 1258, 1275, 2010
TauI GCSGC 1 cut(s) 810
TfiI GAWTC 5 cut(s) 246, 347, 847, 1091, 1777
Tru1I TTAA 5 cut(s) 993, 1583, 1626, 1797, 1857
Tru9I TTAA 5 cut(s) 993, 1583, 1626, 1797, 1857
TscAI CASTG 3 cut(s) 268, 771, 1746
TseFI GTSAC 1 cut(s) 1921
Tsp45I GTSAC 1 cut(s) 1921
TspDTI ATGAA 4 cut(s) 40, 308, 381, 1536
TspGWI ACGGA 4 cut(s) 27, 258, 579, 1997
TspMI CCCGGG 1 cut(s) 1300
TspRI CASTG 3 cut(s) 268, 771, 1746
Tth111I GACNNNGTC 1 cut(s) 1145
Van91I CCANNNNNTGG 1 cut(s) 766
VneI GTGCAC 1 cut(s) 1062
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 2 cut(s) 421, 928
XceI RCATGY 2 cut(s) 1373, 1418
XhoI CTCGAG 1 cut(s) 1179
XmaI CCCGGG 1 cut(s) 1300
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 2 cut(s) 477, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.