MD02G1289300.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000257
Physical Location & Seq
Forward (+)
34555095 .. 34560861
5767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1289300.v1.1.491

Sequence Viewer

Length: 3321 bp
ATGCAGATGAAAATTTTCTGTCCTTCATTTTGCTATAAGGTACTCGTCAATGGCTGCGGTTTCAGCATCGAAACAATGCCAGGGCACGAAGCTCACCCGGTTTTTTATTCTTTCTCCATTGTTCTTGTTCTCAGCATGAGCTTAGCTAGTGAAGCCGTCGATCAGGAAGATAGAAAGACATACATTGTGTACTTGGGATCGCTTCCTAATGATGAGGTGTTCTCGCCATTGTCTCACCACATTGGTATTCTCGAAAGCGTTGTGGAGAGTAGCTCTGCTTCGAATCTGTTCATCAGAAACTATAAGAGGAGCTTCAATGGATTCGCCGCCAAGCTCACTGACCGAGAAAGAGAGAGGCTTGCTAACATGAAGGGAGTCGTCTCTGTGTTTCTGAGCAGAAGCTACCAACTTCAAACAACAAGGTCTTGGGACTTTATTGGGTTCAATGAGAAGATCAAACGAAACTCTACTGTCGAGAGTAGTGATGTCATTATTGGTGTGATCGACAGTGGAATCTGGTCTGAATTGGAGAACTTTAAAGACAAAGGTTTTGGTCCTGCTCCCAAGAAGTGGAAATGTGCTTGTGAAGGCGGCAAAAATTTCACTTGCAATGACAAGATCATTGGAGCAAGGCATTACAATTCATCATCGGCGAGGGATGAGGCAGGTCACGGAACCCACACTGCCTCAATAGCAGTTGGGAACCCCGTTAAGGACGTGAGCTTTTATGGACTAGCACAAGCACTACAAGAGGAGGAGTTCCCTCTGCAAGAATTGCTGCATATAAAGTCTGCAATCATGACGAGTGCTCTTCGGAGGCAATCTTGGCTGCTTGGAAGTGGTACTGCTGCTGATTTTGACCAGGATGCTGTGGCAATTGGAACTTTCCATGCAATGAAGAAAGGGATACTAACGGCACAATCTGCAGGCAACGAATGTCCCAACCTGGGTACTGTATCAAGTGTAGCGCCCTGGATACTTACAGTTGCAGCAAGTAACACAGATCACCGGATCATTACCAAGGTTGTTCTTGGAGATGGAAGGACACTAATTGGGAGTTCGGTTAACACTTTCACAACCAATGGAACAAGTTATCCCTTGGTATATGGAAAAGATGCGTCAAGTAAATGCCAGGAATTGCTTGCTACGATGTGTCTAGAAGGATGCCTAGACAGTGATTTAGTTAAGGGAAAGATTGTAGTATGTGATCAGCTCAATGGACATACAGAGGCTCGTCGATCTGGTGCGAAAGGTTCAATTTTAAGGGATGCTTTGAGAAGTGATGTTGCTTTTATTGTCCCACTACCTGCAACAAGATTAAACTACAAAAACTATGAAGTGGTGAGGTCCTACCTCAACTCCACAGACGATGCTCGAGCCAAGATATTCAAAAGTGAAAGCGTAAAAGATCCTGCTGCACCAATTGTTGCTTCCTTCTCCTCACGGGGACCAAATATAATTTTACCTGAAATTATCAGGCCAGATATAAGTCCCCCAGGAGTAGATATTTTGGCTGCATTTTCACCCGTTGCTCCTGTCCTCTCAGATACCCCAGAAGACAAGAGGCGTGTAAAATACAGCATACTATCTGGCACCTCCATGTCTTGCCCCCACGCTGCCGGTGCAGCTGCATATGTTCGAACATTCCACCCACACTTGTCTCCAGCAGCCATCAAATCGTCTCTTATGACTACAGCTTGGCCCATGCATAATTCTGACAATTATTATCCTGGTGAATTTGCTTATGGTTCTGGACATATCAATCCTTCAAAGGCTGTACATCCGGGTCTTGTCTACGAAACTTCTAAAGACGATTACATAAAGTTTCTCTGCTCGACCTTGGACGAGGCCAGAGTTAGACTTATATCGGGAGATAACAGCTCTTGTCAGACAAGCTCTGACAAAGGATCGGTGAAGGATCTCAATTATCCTTCGCTAGCTGCTATTGTTACACCATTGAAATCATCTAACATTAAATTTAGCAGAACAGTTAAAAATGTTGGCCTTCCAAACTCCACTTACGAGGCCAAAATCCTCCCAACTATGGAAGTTGAAATCAAAGTGGCGCCTAAAGTTCTTTCCTTCAAGTTGTGGAATGAGGAGAAAACTTTCACTGTGACCGTTGTTGGGAAAGATTTTCCTGATGCATCACATGTGTCTGTGCCACTGGTTTGGTCCGATGGAACTCATACTGTTACAAGTCCAATCCTTAGCAGCTCTGCTACAAATTTCTTGATGAGAAGCTACAAAAGGAGCTTCAACGGATTCGTTGCCAAGCTCACTGACCAAGAAAGAGAAAGGCTTGCTAACACAAAGGGAGTTGTCTCTGTGTTTCCGAGCACAACTTACAAGCTTCAAAGAACAAGATCTTGGAATTTCATCGGGCTCAATGAGAAGATCAAACGAAACTCTTCTGCCAAAAGTGATGTTTTGGTCCTGCTCCAGTGGAAAGGAGTTTGTGAAGGAGGCAAAAAATTCACATGCAACAAGATCATTGGAGCTCGATTTTACATTGAGTCCACAGCTAGAGATGAGGTTGGTCATGGAACTCACACTGCATCAACCACAGCAGGGAACCCCGTAAAGGGTGTGAGTTTTTATGGAATGGCCCAAGGCACTGCAAGAAGAGGCATTCCCTCAGCGAGAATTGCTGAATATAAATTTTGTGGTTCTGATGAGTGTGCTATGGATGGGATCTTGGCTGCTTTTGATGACGCTATTGCAGATGGAAAAGGGATACTAACCGCACAATCTGCAGGCAACCAAGGTCCTTCCCTGGGTACTGTATCAAGTGCAGCATCTTGGATACTTTCAGTTGCAGCAAGTAGCACAGATTGCCGAATAGTTGACAAGGTTGTCCTTGGAAATGGAAGGACACTAATTGGGAGTTCGGTGAACTCTTTCACATCTAACGGGACAAGTTATTCTTTGGTATACATAAAAAATGCGTCAAGTAAATGCGAGGAATCACTTGCTACGATGTGTGAAGAAGGATGCCTAGACAGTGATTTAGTTAAGGGAAAGATCGTATTATGTGATCAGTTCAATGGACATAAAGAGGTTCATCGAGCTGGTGCAGAAGGTTCAATTATAAGGGATGTTCTGAGAAATGATGTTGCTTTTATTGTCCCATTGCCTGCAACCAGATTGAGATCCAAAGATTATGAAGTGGTCAGGTTGTACCTCAGCTCCACAGACAATGCTCGAGCCAAGATATTAGATAGTGAAGGCGTAAAAGATCCTTCTGCACCAATTGTTGGCTGCTGCTCCTCACGGGGACCAAATTTAATTTTACCTGCAAAATTATCAAGCCAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1107

Amino Acids

119.63

Weight (kDa)

8.65

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 60 - 138 3.7e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 161 - 570 3e-27 Subtilase family
fn3_6 PF17766 640 - 737 1.9e-23 Fibronectin type-III domain
Inhibitor_I9 PF05922 739 - 786 3e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3092
AasI GACNNNNNNGTC 1 cut(s) 2855
Acc36I ACCTGC 3 cut(s) 656, 1315, 3304
AccB1I GGYRCC 2 cut(s) 1592, 2065
AccB7I CCANNNNNTGG 2 cut(s) 570, 3257
AccI GTMKAC 2 cut(s) 1794, 2934
AciI CCGC 4 cut(s) 57, 327, 591, 2745
AclWI GGATC 8 cut(s) 205, 1019, 1403, 1915, 1926, 2702, 3147, 3233
AcsI RAATTY 9 cut(s) 12, 598, 1736, 1976, 2227, 2374, 2474, 2660, 3283
AcyI GRCGYC 1 cut(s) 2066
AfaI GTAC 7 cut(s) 42, 191, 844, 952, 1779, 2782, 3182
AflIII ACRYGT 1 cut(s) 2152
AjiI CACGTC 1 cut(s) 718
AjnI CCWGG 8 cut(s) 79, 861, 945, 971, 1131, 1495, 1729, 2775
AjuI GAANNNNNNNTTGG 4 cut(s) 1414, 1446, 2353, 2385
AloI GAACNNNNNNTCC 2 cut(s) 1078, 1110
Alw21I GWGCWC 3 cut(s) 811, 2342, 2503
Alw26I GTCTC 5 cut(s) 237, 385, 1665, 1686, 2329
AlwI GGATC 8 cut(s) 205, 1019, 1403, 1915, 1926, 2702, 3147, 3233
Ama87I CYCGRG 2 cut(s) 1374, 3204
AoxI GGCC 6 cut(s) 1478, 1700, 1848, 2002, 2025, 2607
ApoI RAATTY 9 cut(s) 12, 598, 1736, 1976, 2227, 2374, 2474, 2660, 3283
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
Asp700I GAANNNNTTC 5 cut(s) 14, 287, 2108, 2410, 2900
AspLEI GCGC 2 cut(s) 970, 2068
AspS9I GGNCC 9 cut(s) 554, 1347, 1448, 1701, 2175, 2434, 2608, 2768, 3278
AsuC2I CCSGG 2 cut(s) 98, 1785
AsuHPI GGTGA 8 cut(s) 86, 227, 998, 1354, 1515, 1745, 1924, 2905
AsuII TTCGAA 2 cut(s) 281, 1639
AsuNHI GCTAGC 1 cut(s) 1936
AvaI CYCGRG 2 cut(s) 1374, 3204
AvaII GGWCC 7 cut(s) 554, 1347, 1448, 2175, 2434, 2768, 3278
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 2 cut(s) 1592, 2065
BanII GRGCYC 2 cut(s) 2388, 2503
BarI GAAGNNNNNNTAC 2 cut(s) 262, 294
BbsI GAAGAC 1 cut(s) 1563
Bbv12I GWGCWC 3 cut(s) 811, 2342, 2503
BbvCI CCTCAGC 2 cut(s) 2638, 3185
BccI CCATC 5 cut(s) 1031, 1679, 2174, 2684, 2720
BceAI ACGGC 2 cut(s) 140, 930
BciT130I CCWGG 8 cut(s) 81, 863, 947, 973, 1133, 1497, 1731, 2777
BciVI GTATCC 4 cut(s) 900, 969, 2730, 2799
BclI TGATCA 2 cut(s) 1207, 3037
BcnI CCSGG 2 cut(s) 98, 1785
BcoDI GTCTC 5 cut(s) 237, 385, 1665, 1686, 2329
BfaI CTAG 7 cut(s) 147, 734, 1157, 1169, 1937, 2526, 2999
BfmI CTRYAG 3 cut(s) 924, 1692, 2754
BfoI RGCGCY 2 cut(s) 971, 2069
BfuAI ACCTGC 3 cut(s) 656, 1315, 3304
BfuI GTATCC 4 cut(s) 900, 969, 2730, 2799
BglII AGATCT 1 cut(s) 2366
BlpI GCTNAGC 1 cut(s) 142
Bme18I GGWCC 7 cut(s) 554, 1347, 1448, 2175, 2434, 2768, 3278
BmeT110I CYCGRG 2 cut(s) 1374, 3204
BmgBI CACGTC 1 cut(s) 718
BmgT120I GGNCC 9 cut(s) 554, 1347, 1448, 1701, 2175, 2434, 2608, 2768, 3278
BmiI GGNNCC 7 cut(s) 676, 704, 1449, 1594, 2067, 2576, 3279
BmtI GCTAGC 1 cut(s) 1940
BpiI GAAGAC 1 cut(s) 1563
BplI GAGNNNNNCTC 4 cut(s) 206, 238, 257, 289
BpmI CTGGAG 2 cut(s) 1647, 2426
Bpu10I CCTNAGC 3 cut(s) 2210, 2638, 3185
Bpu1102I GCTNAGC 1 cut(s) 142
Bpu14I TTCGAA 2 cut(s) 281, 1639
BpuMI CCSGG 2 cut(s) 98, 1785
BsaBI GATNNNNATC 1 cut(s) 3151
BsaHI GRCGYC 1 cut(s) 2066
BsaWI WCCGGW 1 cut(s) 1008
BsaXI ACNNNNNCTCC 4 cut(s) 1343, 1373, 3173, 3203
Bse118I RCCGGY 1 cut(s) 1619
Bse1I ACTGG 2 cut(s) 2172, 2443
Bse3DI GCAATG 3 cut(s) 616, 900, 3131
Bse8I GATNNNNATC 1 cut(s) 3151
BseBI CCWGG 8 cut(s) 81, 863, 947, 973, 1133, 1497, 1731, 2777
BseGI GGATG 8 cut(s) 664, 871, 1169, 1273, 1780, 2695, 2999, 3103
BseJI GATNNNNATC 1 cut(s) 3151
BseMI GCAATG 3 cut(s) 616, 900, 3131
BseMII CTCAG 6 cut(s) 145, 383, 1557, 2652, 3095, 3199
BseNI ACTGG 2 cut(s) 2172, 2443
BseRI GAGGAG 6 cut(s) 322, 767, 770, 1429, 2114, 3259
BseSI GKGCMC 1 cut(s) 87
BsgI GTGCAG 5 cut(s) 1401, 1644, 2814, 3096, 3231
BshFI GGCC 6 cut(s) 1480, 1702, 1850, 2004, 2027, 2609
BshNI GGYRCC 2 cut(s) 1592, 2065
BsiHKAI GWGCWC 3 cut(s) 811, 2342, 2503
BsiHKCI CYCGRG 2 cut(s) 1374, 3204
BsiSI CCGG 4 cut(s) 98, 1009, 1620, 1784
BslFI GGGAC 8 cut(s) 443, 924, 1283, 1461, 1476, 2929, 3113, 3291
BsmAI GTCTC 5 cut(s) 237, 385, 1665, 1686, 2329
BsmBI CGTCTC 2 cut(s) 385, 1686
BsmFI GGGAC 8 cut(s) 443, 924, 1283, 1461, 1476, 2929, 3113, 3291
BsmI GAATGC 1 cut(s) 2631
BsnI GGCC 6 cut(s) 1480, 1702, 1850, 2004, 2027, 2609
BsoBI CYCGRG 2 cut(s) 1374, 3204
Bsp119I TTCGAA 2 cut(s) 281, 1639
Bsp1286I GDGCHC 5 cut(s) 87, 811, 2342, 2388, 2503
Bsp1407I TGTACA 1 cut(s) 1777
Bsp1720I GCTNAGC 1 cut(s) 142
BspACI CCGC 4 cut(s) 57, 327, 591, 2745
BspANI GGCC 6 cut(s) 1480, 1702, 1850, 2004, 2027, 2609
BspCNI CTCAG 6 cut(s) 144, 384, 1556, 2651, 3096, 3198
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 7 cut(s) 676, 704, 1449, 1594, 2067, 2576, 3279
BspMAI CTGCAG 2 cut(s) 928, 2758
BspMI ACCTGC 3 cut(s) 656, 1315, 3304
BspOI GCTAGC 1 cut(s) 1940
BspPI GGATC 8 cut(s) 205, 1019, 1403, 1915, 1926, 2702, 3147, 3233
BspQI GCTCTTC 1 cut(s) 816
BspT104I TTCGAA 2 cut(s) 281, 1639
BspT107I GGYRCC 2 cut(s) 1592, 2065
BsrDI GCAATG 3 cut(s) 616, 900, 3131
BsrFI RCCGGY 1 cut(s) 1619
BsrGI TGTACA 1 cut(s) 1777
BsrI ACTGG 2 cut(s) 2172, 2443
BssAI RCCGGY 1 cut(s) 1619
BssNAI GTATAC 1 cut(s) 2935
BssNI GRCGYC 1 cut(s) 2066
BssT1I CCWWGG 6 cut(s) 1020, 1098, 1839, 2611, 2764, 2860
Bst1107I GTATAC 1 cut(s) 2935
Bst2UI CCWGG 8 cut(s) 81, 863, 947, 973, 1133, 1497, 1731, 2777
Bst6I CTCTTC 3 cut(s) 816, 2416, 2620
BstACI GRCGYC 1 cut(s) 2066
BstAPI GCANNNNNTGC 4 cut(s) 775, 923, 2753, 2835
BstAUI TGTACA 1 cut(s) 1777
BstBI TTCGAA 2 cut(s) 281, 1639
BstC8I GCNNGC 7 cut(s) 360, 928, 1143, 1938, 2304, 2758, 3138
BstDEI CTNAG 8 cut(s) 131, 142, 392, 1543, 2210, 2638, 3104, 3185
BstF5I GGATG 8 cut(s) 664, 871, 1169, 1273, 1780, 2695, 2999, 3103
BstH2I RGCGCY 2 cut(s) 971, 2069
BstHHI GCGC 2 cut(s) 970, 2068
BstMAI GTCTC 5 cut(s) 237, 385, 1665, 1686, 2329
BstNI CCWGG 8 cut(s) 81, 863, 947, 973, 1133, 1497, 1731, 2777
BstNSI RCATGY 2 cut(s) 2156, 2484
BstSFI CTRYAG 3 cut(s) 924, 1692, 2754
BstSLI GKGCMC 1 cut(s) 87
BstV2I GAAGAC 1 cut(s) 1563
BstX2I RGATCY 6 cut(s) 1408, 1918, 2366, 2694, 3152, 3238
BstXI CCANNNNNNTGG 1 cut(s) 2172
BstYI RGATCY 6 cut(s) 1408, 1918, 2366, 2694, 3152, 3238
BstZ17I GTATAC 1 cut(s) 2935
BsuI GTATCC 4 cut(s) 900, 969, 2730, 2799
BsuRI GGCC 6 cut(s) 1480, 1702, 1850, 2004, 2027, 2609
BtrI CACGTC 1 cut(s) 718
BtsCI GGATG 8 cut(s) 664, 871, 1169, 1273, 1780, 2695, 2999, 3103
BtsI GCAGTG 3 cut(s) 681, 2553, 2616
BveI ACCTGC 3 cut(s) 656, 1315, 3304
Cac8I GCNNGC 7 cut(s) 360, 928, 1143, 1938, 2304, 2758, 3138
CciI TCATGA 1 cut(s) 798
CfoI GCGC 2 cut(s) 970, 2068
Cfr10I RCCGGY 1 cut(s) 1619
Cfr13I GGNCC 9 cut(s) 554, 1347, 1448, 1701, 2175, 2434, 2608, 2768, 3278
CseI GACGC 3 cut(s) 1107, 2723, 2937
Csp6I GTAC 7 cut(s) 41, 190, 843, 951, 1778, 2781, 3181
CviAII CATG 9 cut(s) 136, 367, 799, 890, 1600, 1705, 2153, 2481, 2543
CviQI GTAC 7 cut(s) 41, 190, 843, 951, 1778, 2781, 3181
DdeI CTNAG 8 cut(s) 131, 142, 392, 1543, 2210, 2638, 3104, 3185
DinI GGCGCC 1 cut(s) 2067
DraI TTTAAA 1 cut(s) 538
DrdI GACNNNNNNGTC 1 cut(s) 2855
DseDI GACNNNNNNGTC 1 cut(s) 2855
Eam1104I CTCTTC 3 cut(s) 816, 2416, 2620
EarI CTCTTC 3 cut(s) 816, 2416, 2620
Ecl136II GAGCTC 1 cut(s) 2501
Eco130I CCWWGG 6 cut(s) 1020, 1098, 1839, 2611, 2764, 2860
Eco24I GRGCYC 2 cut(s) 2388, 2503
Eco47I GGWCC 7 cut(s) 554, 1347, 1448, 2175, 2434, 2768, 3278
Eco53kI GAGCTC 1 cut(s) 2501
Eco88I CYCGRG 2 cut(s) 1374, 3204
EcoICRI GAGCTC 1 cut(s) 2501
EcoO109I RGGNCCY 2 cut(s) 1347, 2768
EcoRII CCWGG 8 cut(s) 79, 861, 945, 971, 1131, 1495, 1729, 2775
EcoT14I CCWWGG 6 cut(s) 1020, 1098, 1839, 2611, 2764, 2860
EcoT22I ATGCAT 2 cut(s) 1710, 2149
EcoT38I GRGCYC 2 cut(s) 2388, 2503
EgeI GGCGCC 1 cut(s) 2067
EheI GGCGCC 1 cut(s) 2067
ErhI CCWWGG 6 cut(s) 1020, 1098, 1839, 2611, 2764, 2860
Esp3I CGTCTC 2 cut(s) 385, 1686
FaeI CATG 9 cut(s) 139, 370, 802, 893, 1603, 1708, 2156, 2484, 2546
FalI AAGNNNNNCTT 6 cut(s) 296, 328, 1255, 1287, 2911, 2943
FaqI GGGAC 8 cut(s) 443, 924, 1283, 1461, 1476, 2929, 3113, 3291
FatI CATG 9 cut(s) 135, 366, 798, 889, 1599, 1704, 2152, 2480, 2542
FauNDI CATATG 1 cut(s) 1633
FbaI TGATCA 2 cut(s) 1207, 3037
FblI GTMKAC 2 cut(s) 1794, 2934
FokI GGATG 8 cut(s) 671, 878, 1176, 1280, 1767, 2702, 3006, 3110
FriOI GRGCYC 2 cut(s) 2388, 2503
FspBI CTAG 7 cut(s) 147, 734, 1157, 1169, 1937, 2526, 2999
GlaI GCGC 2 cut(s) 969, 2067
GsuI CTGGAG 2 cut(s) 1647, 2426
HaeII RGCGCY 2 cut(s) 971, 2069
HaeIII GGCC 6 cut(s) 1480, 1702, 1850, 2004, 2027, 2609
HapII CCGG 4 cut(s) 98, 1009, 1620, 1784
HgaI GACGC 3 cut(s) 1107, 2723, 2937
HhaI GCGC 2 cut(s) 970, 2068
Hin1I GRCGYC 1 cut(s) 2066
Hin1II CATG 9 cut(s) 139, 370, 802, 893, 1603, 1708, 2156, 2484, 2546
Hin6I GCGC 2 cut(s) 968, 2066
HinP1I GCGC 2 cut(s) 968, 2066
HincII GTYRAC 2 cut(s) 1066, 2848
HindII GTYRAC 2 cut(s) 1066, 2848
HindIII AAGCTT 1 cut(s) 2351
HinfI GANTC 7 cut(s) 283, 321, 375, 513, 2265, 2516, 2966
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 4 cut(s) 98, 1009, 1620, 1784
HphI GGTGA 8 cut(s) 86, 227, 998, 1354, 1515, 1745, 1924, 2905
Hpy166II GTNNAC 7 cut(s) 190, 1066, 1795, 2520, 2848, 2896, 2935
Hpy188III TCNNGA 9 cut(s) 164, 251, 475, 799, 1157, 1752, 1869, 2141, 2233
Hpy8I GTNNAC 7 cut(s) 190, 1066, 1795, 2520, 2848, 2896, 2935
Hpy99I CGWCG 2 cut(s) 161, 1239
HpyCH4IV ACGT 1 cut(s) 717
HpyF3I CTNAG 8 cut(s) 131, 142, 392, 1543, 2210, 2638, 3104, 3185
HpySE526I ACGT 1 cut(s) 717
Hsp92I GRCGYC 1 cut(s) 2066
Hsp92II CATG 9 cut(s) 139, 370, 802, 893, 1603, 1708, 2156, 2484, 2546
HspAI GCGC 2 cut(s) 968, 2066
KasI GGCGCC 1 cut(s) 2065
Ksp22I TGATCA 2 cut(s) 1207, 3037
KspAI GTTAAC 1 cut(s) 1066
LguI GCTCTTC 1 cut(s) 816
LmnI GCTCC 9 cut(s) 309, 565, 626, 1537, 2253, 2445, 2498, 3194, 3272
MaeI CTAG 7 cut(s) 147, 734, 1157, 1169, 1937, 2526, 2999
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 5 cut(s) 668, 995, 1948, 2116, 2194
MboII GAAGA 9 cut(s) 179, 463, 803, 910, 1568, 2403, 2407, 2637, 2999
MfeI CAATTG 3 cut(s) 876, 1422, 3252
MflI RGATCY 6 cut(s) 1408, 1918, 2366, 2694, 3152, 3238
MhlI GDGCHC 5 cut(s) 87, 811, 2342, 2388, 2503
Mly113I GGCGCC 1 cut(s) 2066
MlyI GAGTC 2 cut(s) 384, 2525
Mph1103I ATGCAT 2 cut(s) 1710, 2149
MroXI GAANNNNTTC 5 cut(s) 14, 287, 2108, 2410, 2900
MslI CAYNNNNRTG 1 cut(s) 1598
MspA1I CMGCKG 1 cut(s) 1628
MspI CCGG 4 cut(s) 98, 1009, 1620, 1784
MunI CAATTG 3 cut(s) 876, 1422, 3252
Mva1269I GAATGC 1 cut(s) 2631
MvaI CCWGG 8 cut(s) 81, 863, 947, 973, 1133, 1497, 1731, 2777
NarI GGCGCC 1 cut(s) 2066
NciI CCSGG 2 cut(s) 98, 1785
NdeI CATATG 1 cut(s) 1633
NheI GCTAGC 1 cut(s) 1936
NlaIII CATG 9 cut(s) 139, 370, 802, 893, 1603, 1708, 2156, 2484, 2546
NlaIV GGNNCC 7 cut(s) 676, 704, 1449, 1594, 2067, 2576, 3279
NmuCI GTSAC 2 cut(s) 668, 2116
NsiI ATGCAT 2 cut(s) 1710, 2149
NspI RCATGY 2 cut(s) 2156, 2484
NspV TTCGAA 2 cut(s) 281, 1639
PaeR7I CTCGAG 2 cut(s) 1374, 3204
PagI TCATGA 1 cut(s) 798
PasI CCCWGGG 1 cut(s) 2776
PciI ACATGT 1 cut(s) 2152
PciSI GCTCTTC 1 cut(s) 816
PctI GAATGC 1 cut(s) 2631
PdmI GAANNNNTTC 5 cut(s) 14, 287, 2108, 2410, 2900
PfeI GAWTC 5 cut(s) 283, 321, 513, 2265, 2966
PflMI CCANNNNNTGG 2 cut(s) 570, 3257
PleI GAGTC 2 cut(s) 383, 2524
PluTI GGCGCC 1 cut(s) 2069
PpsI GAGTC 2 cut(s) 383, 2524
PpuMI RGGWCCY 2 cut(s) 1347, 2768
PscI ACATGT 1 cut(s) 2152
PsiI TTATAA 1 cut(s) 3092
Psp124BI GAGCTC 1 cut(s) 2503
Psp5II RGGWCCY 2 cut(s) 1347, 2768
Psp6I CCWGG 8 cut(s) 79, 861, 945, 971, 1131, 1495, 1729, 2775
PspGI CCWGG 8 cut(s) 79, 861, 945, 971, 1131, 1495, 1729, 2775
PspN4I GGNNCC 7 cut(s) 676, 704, 1449, 1594, 2067, 2576, 3279
PspPI GGNCC 9 cut(s) 554, 1347, 1448, 1701, 2175, 2434, 2608, 2768, 3278
PspPPI RGGWCCY 2 cut(s) 1347, 2768
PspXI VCTCGAGB 2 cut(s) 1374, 3204
PstI CTGCAG 2 cut(s) 928, 2758
PsuI RGATCY 6 cut(s) 1408, 1918, 2366, 2694, 3152, 3238
PvuII CAGCTG 1 cut(s) 1628
RsaI GTAC 7 cut(s) 42, 191, 844, 952, 1779, 2782, 3182
RsaNI GTAC 7 cut(s) 41, 190, 843, 951, 1778, 2781, 3181
RseI CAYNNNNRTG 1 cut(s) 1598
SacI GAGCTC 1 cut(s) 2503
SapI GCTCTTC 1 cut(s) 816
Sau96I GGNCC 9 cut(s) 554, 1347, 1448, 1701, 2175, 2434, 2608, 2768, 3278
SchI GAGTC 2 cut(s) 384, 2525
SduI GDGCHC 5 cut(s) 87, 811, 2342, 2388, 2503
SfcI CTRYAG 3 cut(s) 924, 1692, 2754
SfoI GGCGCC 1 cut(s) 2067
Sfr274I CTCGAG 2 cut(s) 1374, 3204
SfuI TTCGAA 2 cut(s) 281, 1639
SinI GGWCC 7 cut(s) 554, 1347, 1448, 2175, 2434, 2768, 3278
SlaI CTCGAG 2 cut(s) 1374, 3204
SmiMI CAYNNNNRTG 1 cut(s) 1598
SmlI CTYRAG 2 cut(s) 1374, 3204
SmoI CTYRAG 2 cut(s) 1374, 3204
SsiI CCGC 4 cut(s) 57, 327, 591, 2745
SspDI GGCGCC 1 cut(s) 2065
SspMI CTAG 7 cut(s) 147, 734, 1157, 1169, 1937, 2526, 2999
SstI GAGCTC 1 cut(s) 2503
StyI CCWWGG 6 cut(s) 1020, 1098, 1839, 2611, 2764, 2860
TaiI ACGT 1 cut(s) 720
TaqII GACCGA 1 cut(s) 357
TatI WGTACW 2 cut(s) 189, 1777
TauI GCSGC 2 cut(s) 329, 594
TfiI GAWTC 5 cut(s) 283, 321, 513, 2265, 2966
TseFI GTSAC 2 cut(s) 668, 2116
Tsp45I GTSAC 2 cut(s) 668, 2116
TspGWI ACGGA 2 cut(s) 687, 2277
Van91I CCANNNNNTGG 2 cut(s) 570, 3257
VpaK11BI GGWCC 7 cut(s) 554, 1347, 1448, 2175, 2434, 2768, 3278
XapI RAATTY 9 cut(s) 12, 598, 1736, 1976, 2227, 2374, 2474, 2660, 3283
XbaI TCTAGA 1 cut(s) 1156
XceI RCATGY 2 cut(s) 2156, 2484
XhoI CTCGAG 2 cut(s) 1374, 3204
XmiI GTMKAC 2 cut(s) 1794, 2934
XmnI GAANNNNTTC 5 cut(s) 14, 287, 2108, 2410, 2900
XspI CTAG 7 cut(s) 147, 734, 1157, 1169, 1937, 2526, 2999
Zsp2I ATGCAT 2 cut(s) 1710, 2149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.