subtilase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 222, 726 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 743 |
| AccB1I | GGYRCC | 2 cut(s) | 961, 2117 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 889 |
| AccBSI | CCGCTC | 1 cut(s) | 1739 |
| AccI | GTMKAC | 3 cut(s) | 108, 1098, 1996 |
| AccII | CGCG | 1 cut(s) | 1277 |
| AclI | AACGTT | 1 cut(s) | 1625 |
| AclWI | GGATC | 6 cut(s) | 190, 210, 1498, 1648, 1745, 2038 |
| AcoI | YGGCCR | 3 cut(s) | 891, 1682, 1719 |
| AcsI | RAATTY | 4 cut(s) | 349, 768, 1060, 1724 |
| AcuI | CTGAAG | 3 cut(s) | 51, 442, 1119 |
| AcyI | GRCGYC | 2 cut(s) | 962, 2118 |
| AdeI | CACNNNGTG | 1 cut(s) | 2182 |
| AfaI | GTAC | 6 cut(s) | 379, 557, 847, 1088, 1559, 2084 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 448, 832, 889, 1257 |
| AflIII | ACRYGT | 2 cut(s) | 1174, 1549 |
| AgsI | TTSAA | 7 cut(s) | 173, 235, 1116, 1468, 1581, 1828, 1960 |
| AhlI | ACTAGT | 2 cut(s) | 161, 1777 |
| AjnI | CCWGG | 5 cut(s) | 198, 739, 1153, 1486, 1771 |
| AleI | CACNNNNGTG | 1 cut(s) | 1156 |
| Alw21I | GWGCWC | 3 cut(s) | 121, 553, 758 |
| Alw26I | GTCTC | 6 cut(s) | 19, 29, 1306, 1382, 1647, 2149 |
| Alw44I | GTGCAC | 2 cut(s) | 117, 754 |
| AlwI | GGATC | 6 cut(s) | 190, 210, 1498, 1648, 1745, 2038 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1304 |
| AoxI | GGCC | 6 cut(s) | 358, 891, 1438, 1682, 1719, 1741 |
| ApaLI | GTGCAC | 2 cut(s) | 117, 754 |
| ApeKI | GCWGC | 7 cut(s) | 245, 707, 983, 1370, 1505, 1760, 1869 |
| ApoI | RAATTY | 4 cut(s) | 349, 768, 1060, 1724 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2096 |
| AspLEI | GCGC | 4 cut(s) | 920, 964, 1486, 2120 |
| AspS9I | GGNCC | 7 cut(s) | 358, 476, 932, 1439, 1741, 2123, 2163 |
| AsuC2I | CCSGG | 3 cut(s) | 250, 1141, 1218 |
| AsuHPI | GGTGA | 6 cut(s) | 172, 1003, 1143, 1622, 1801, 1874 |
| AvaII | GGWCC | 4 cut(s) | 476, 932, 2123, 2163 |
| BaeGI | GKGCMC | 2 cut(s) | 121, 758 |
| BalI | TGGCCA | 2 cut(s) | 893, 1684 |
| BanI | GGYRCC | 2 cut(s) | 961, 2117 |
| BanII | GRGCYC | 1 cut(s) | 675 |
| BauI | CACGAG | 2 cut(s) | 129, 1434 |
| BbsI | GAAGAC | 2 cut(s) | 1278, 1279 |
| Bbv12I | GWGCWC | 3 cut(s) | 121, 553, 758 |
| BbvI | GCAGC | 7 cut(s) | 257, 694, 970, 1382, 1492, 1772, 1881 |
| BccI | CCATC | 4 cut(s) | 162, 758, 1673, 2162 |
| BceAI | ACGGC | 3 cut(s) | 949, 1048, 1226 |
| BciT130I | CCWGG | 5 cut(s) | 200, 741, 1155, 1488, 1773 |
| BciVI | GTATCC | 1 cut(s) | 832 |
| BclI | TGATCA | 1 cut(s) | 82 |
| BcnI | CCSGG | 3 cut(s) | 250, 1141, 1218 |
| BcoDI | GTCTC | 6 cut(s) | 19, 29, 1306, 1382, 1647, 2149 |
| BcuI | ACTAGT | 2 cut(s) | 161, 1777 |
| BfoI | RGCGCY | 3 cut(s) | 965, 1487, 2121 |
| BfuI | GTATCC | 1 cut(s) | 832 |
| Bme1390I | CCNGG | 8 cut(s) | 200, 250, 741, 1141, 1155, 1218, 1488, 1773 |
| Bme18I | GGWCC | 4 cut(s) | 476, 932, 2123, 2163 |
| BmgT120I | GGNCC | 7 cut(s) | 358, 476, 932, 1439, 1741, 2123, 2163 |
| BmiI | GGNNCC | 5 cut(s) | 963, 1240, 1262, 2119, 2125 |
| BmrFI | CCNGG | 8 cut(s) | 200, 250, 741, 1141, 1155, 1218, 1488, 1773 |
| BmsI | GCATC | 7 cut(s) | 635, 778, 826, 1112, 1117, 1256, 1703 |
| BpiI | GAAGAC | 2 cut(s) | 1278, 1279 |
| BpuEI | CTTGAG | 3 cut(s) | 347, 1212, 1920 |
| BpuMI | CCSGG | 3 cut(s) | 250, 1141, 1218 |
| BsaHI | GRCGYC | 2 cut(s) | 962, 2118 |
| BsaI | GGTCTC | 1 cut(s) | 1647 |
| BsaJI | CCNNGG | 6 cut(s) | 739, 826, 888, 894, 965, 1154 |
| BsaWI | WCCGGW | 1 cut(s) | 182 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 448, 832, 889, 1257 |
| Bse118I | RCCGGY | 1 cut(s) | 2120 |
| Bse1I | ACTGG | 2 cut(s) | 1298, 1840 |
| BseBI | CCWGG | 5 cut(s) | 200, 741, 1155, 1488, 1773 |
| BseDI | CCNNGG | 6 cut(s) | 739, 826, 888, 894, 965, 1154 |
| BseGI | GGATG | 4 cut(s) | 626, 841, 1247, 1768 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 448, 832, 889, 1257 |
| BseMII | CTCAG | 1 cut(s) | 576 |
| BseNI | ACTGG | 2 cut(s) | 1298, 1840 |
| BseRI | GAGGAG | 3 cut(s) | 17, 471, 869 |
| BseSI | GKGCMC | 2 cut(s) | 121, 758 |
| BseXI | GCAGC | 7 cut(s) | 257, 694, 970, 1382, 1492, 1772, 1881 |
| BseYI | CCCAGC | 1 cut(s) | 1206 |
| Bsh1236I | CGCG | 1 cut(s) | 1277 |
| BshFI | GGCC | 6 cut(s) | 360, 893, 1440, 1684, 1721, 1743 |
| BshNI | GGYRCC | 2 cut(s) | 961, 2117 |
| BsiHKAI | GWGCWC | 3 cut(s) | 121, 553, 758 |
| BsiSI | CCGG | 7 cut(s) | 183, 250, 1140, 1218, 1472, 2057, 2121 |
| BslFI | GGGAC | 6 cut(s) | 136, 611, 621, 1508, 1575, 2109 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 448, 832, 889, 1257 |
| BsmAI | GTCTC | 6 cut(s) | 19, 29, 1306, 1382, 1647, 2149 |
| BsmFI | GGGAC | 6 cut(s) | 136, 611, 621, 1508, 1575, 2109 |
| BsmI | GAATGC | 1 cut(s) | 1696 |
| BsnI | GGCC | 6 cut(s) | 360, 893, 1440, 1684, 1721, 1743 |
| Bso31I | GGTCTC | 1 cut(s) | 1647 |
| Bsp1286I | GDGCHC | 4 cut(s) | 121, 553, 675, 758 |
| Bsp19I | CCATGG | 1 cut(s) | 888 |
| BspANI | GGCC | 6 cut(s) | 360, 893, 1440, 1684, 1721, 1743 |
| BspCNI | CTCAG | 1 cut(s) | 577 |
| BspFNI | CGCG | 1 cut(s) | 1277 |
| BspHI | TCATGA | 2 cut(s) | 1456, 1668 |
| BspLI | GGNNCC | 5 cut(s) | 963, 1240, 1262, 2119, 2125 |
| BspPI | GGATC | 6 cut(s) | 190, 210, 1498, 1648, 1745, 2038 |
| BspQI | GCTCTTC | 1 cut(s) | 977 |
| BspT107I | GGYRCC | 2 cut(s) | 961, 2117 |
| BspTNI | GGTCTC | 1 cut(s) | 1647 |
| BsrBI | CCGCTC | 1 cut(s) | 1739 |
| BsrFI | RCCGGY | 1 cut(s) | 2120 |
| BsrI | ACTGG | 2 cut(s) | 1298, 1840 |
| BssAI | RCCGGY | 1 cut(s) | 2120 |
| BssECI | CCNNGG | 6 cut(s) | 739, 826, 888, 894, 965, 1154 |
| BssNAI | GTATAC | 2 cut(s) | 109, 1099 |
| BssNI | GRCGYC | 2 cut(s) | 962, 2118 |
| BssSI | CACGAG | 2 cut(s) | 129, 1434 |
| BssT1I | CCWWGG | 2 cut(s) | 888, 894 |
| Bst1107I | GTATAC | 2 cut(s) | 109, 1099 |
| Bst2BI | CACGAG | 2 cut(s) | 129, 1434 |
| Bst2UI | CCWGG | 5 cut(s) | 200, 741, 1155, 1488, 1773 |
| Bst6I | CTCTTC | 2 cut(s) | 977, 1435 |
| BstACI | GRCGYC | 2 cut(s) | 962, 2118 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1875 |
| BstC8I | GCNNGC | 4 cut(s) | 73, 617, 867, 1741 |
| BstDEI | CTNAG | 1 cut(s) | 585 |
| BstDSI | CCRYGG | 2 cut(s) | 888, 965 |
| BstEII | GGTNACC | 1 cut(s) | 2104 |
| BstF5I | GGATG | 4 cut(s) | 626, 841, 1247, 1768 |
| BstFNI | CGCG | 1 cut(s) | 1277 |
| BstH2I | RGCGCY | 3 cut(s) | 965, 1487, 2121 |
| BstHHI | GCGC | 4 cut(s) | 920, 964, 1486, 2120 |
| BstMAI | GTCTC | 6 cut(s) | 19, 29, 1306, 1382, 1647, 2149 |
| BstNI | CCWGG | 5 cut(s) | 200, 741, 1155, 1488, 1773 |
| BstNSI | RCATGY | 3 cut(s) | 145, 659, 1553 |
| BstPI | GGTNACC | 1 cut(s) | 2104 |
| BstSCI | CCNGG | 8 cut(s) | 198, 248, 739, 1139, 1153, 1216, 1486, 1771 |
| BstSLI | GKGCMC | 2 cut(s) | 121, 758 |
| BstUI | CGCG | 1 cut(s) | 1277 |
| BstV1I | GCAGC | 7 cut(s) | 257, 694, 970, 1382, 1492, 1772, 1881 |
| BstV2I | GAAGAC | 2 cut(s) | 1278, 1279 |
| BstX2I | RGATCY | 1 cut(s) | 2030 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1601 |
| BstYI | RGATCY | 1 cut(s) | 2030 |
| BstZ17I | GTATAC | 2 cut(s) | 109, 1099 |
| BsuI | GTATCC | 1 cut(s) | 832 |
| BsuRI | GGCC | 6 cut(s) | 360, 893, 1440, 1684, 1721, 1743 |
| BtgI | CCRYGG | 2 cut(s) | 888, 965 |
| BtgZI | GCGATG | 1 cut(s) | 1140 |
| BtsCI | GGATG | 4 cut(s) | 626, 841, 1247, 1768 |
| BtsIMutI | CAGTG | 4 cut(s) | 436, 906, 975, 1833 |
| Cac8I | GCNNGC | 4 cut(s) | 73, 617, 867, 1741 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1304 |
| CciI | TCATGA | 2 cut(s) | 1456, 1668 |
| CfoI | GCGC | 4 cut(s) | 920, 964, 1486, 2120 |
| Cfr10I | RCCGGY | 1 cut(s) | 2120 |
| Cfr13I | GGNCC | 7 cut(s) | 358, 476, 932, 1439, 1741, 2123, 2163 |
| CseI | GACGC | 1 cut(s) | 1283 |
| Csp6I | GTAC | 6 cut(s) | 378, 556, 846, 1087, 1558, 2083 |
| CviQI | GTAC | 6 cut(s) | 378, 556, 846, 1087, 1558, 2083 |
| DdeI | CTNAG | 1 cut(s) | 585 |
| DinI | GGCGCC | 2 cut(s) | 963, 2119 |
| DraI | TTTAAA | 1 cut(s) | 2073 |
| DraIII | CACNNNGTG | 1 cut(s) | 2182 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 743 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 743 |
| EaeI | YGGCCR | 3 cut(s) | 891, 1682, 1719 |
| Eam1104I | CTCTTC | 2 cut(s) | 977, 1435 |
| EarI | CTCTTC | 2 cut(s) | 977, 1435 |
| Eco130I | CCWWGG | 2 cut(s) | 888, 894 |
| Eco24I | GRGCYC | 1 cut(s) | 675 |
| Eco31I | GGTCTC | 1 cut(s) | 1647 |
| Eco47I | GGWCC | 4 cut(s) | 476, 932, 2123, 2163 |
| Eco57I | CTGAAG | 3 cut(s) | 51, 442, 1119 |
| Eco91I | GGTNACC | 1 cut(s) | 2104 |
| EcoO65I | GGTNACC | 1 cut(s) | 2104 |
| EcoRI | GAATTC | 1 cut(s) | 768 |
| EcoRII | CCWGG | 5 cut(s) | 198, 739, 1153, 1486, 1771 |
| EcoT14I | CCWWGG | 2 cut(s) | 888, 894 |
| EcoT22I | ATGCAT | 1 cut(s) | 1696 |
| EcoT38I | GRGCYC | 1 cut(s) | 675 |
| EgeI | GGCGCC | 2 cut(s) | 963, 2119 |
| EheI | GGCGCC | 2 cut(s) | 963, 2119 |
| ErhI | CCWWGG | 2 cut(s) | 888, 894 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2081, 2113 |
| FaqI | GGGAC | 6 cut(s) | 136, 611, 621, 1508, 1575, 2109 |
| FauI | CCCGC | 1 cut(s) | 1732 |
| FauNDI | CATATG | 1 cut(s) | 1342 |
| FbaI | TGATCA | 1 cut(s) | 82 |
| FblI | GTMKAC | 3 cut(s) | 108, 1098, 1996 |
| FokI | GGATG | 4 cut(s) | 613, 848, 1234, 1755 |
| FriOI | GRGCYC | 1 cut(s) | 675 |
| GlaI | GCGC | 4 cut(s) | 919, 963, 1485, 2119 |
| GsaI | CCCAGC | 1 cut(s) | 1210 |
| HaeII | RGCGCY | 3 cut(s) | 965, 1487, 2121 |
| HaeIII | GGCC | 6 cut(s) | 360, 893, 1440, 1684, 1721, 1743 |
| HapII | CCGG | 7 cut(s) | 183, 250, 1140, 1218, 1472, 2057, 2121 |
| HgaI | GACGC | 1 cut(s) | 1283 |
| HhaI | GCGC | 4 cut(s) | 920, 964, 1486, 2120 |
| Hin1I | GRCGYC | 2 cut(s) | 962, 2118 |
| Hin6I | GCGC | 4 cut(s) | 918, 962, 1484, 2118 |
| HinP1I | GCGC | 4 cut(s) | 918, 962, 1484, 2118 |
| HincII | GTYRAC | 1 cut(s) | 136 |
| HindII | GTYRAC | 1 cut(s) | 136 |
| HindIII | AAGCTT | 1 cut(s) | 539 |
| HpaII | CCGG | 7 cut(s) | 183, 250, 1140, 1218, 1472, 2057, 2121 |
| HphI | GGTGA | 6 cut(s) | 172, 1003, 1143, 1622, 1801, 1874 |
| Hpy188III | TCNNGA | 9 cut(s) | 86, 173, 584, 935, 952, 1163, 1457, 1669, 1944 |
| Hpy99I | CGWCG | 2 cut(s) | 1247, 1994 |
| HpyAV | CCTTC | 6 cut(s) | 135, 362, 1656, 1932, 1966, 2107 |
| HpyCH4IV | ACGT | 7 cut(s) | 341, 376, 940, 1174, 1596, 1625, 1989 |
| HpyCH4V | TGCA | 8 cut(s) | 119, 507, 531, 710, 756, 839, 1694, 1878 |
| HpyF3I | CTNAG | 1 cut(s) | 585 |
| HpySE526I | ACGT | 7 cut(s) | 341, 376, 940, 1174, 1596, 1625, 1989 |
| Hsp92I | GRCGYC | 2 cut(s) | 962, 2118 |
| HspAI | GCGC | 4 cut(s) | 918, 962, 1484, 2118 |
| KasI | GGCGCC | 2 cut(s) | 961, 2117 |
| Ksp22I | TGATCA | 1 cut(s) | 82 |
| LguI | GCTCTTC | 1 cut(s) | 977 |
| LmnI | GCTCC | 4 cut(s) | 548, 1238, 1414, 1736 |
| Lsp1109I | GCAGC | 7 cut(s) | 257, 694, 970, 1382, 1492, 1772, 1881 |
| LweI | GCATC | 7 cut(s) | 635, 778, 826, 1112, 1117, 1256, 1703 |
| MaeII | ACGT | 7 cut(s) | 341, 376, 940, 1174, 1596, 1625, 1989 |
| MaeIII | GTNAC | 5 cut(s) | 178, 734, 1963, 1981, 2104 |
| MbiI | CCGCTC | 1 cut(s) | 1739 |
| MboII | GAAGA | 9 cut(s) | 161, 964, 1062, 1125, 1279, 1283, 1328, 1422, 2101 |
| MfeI | CAATTG | 1 cut(s) | 1041 |
| MflI | RGATCY | 1 cut(s) | 2030 |
| MhlI | GDGCHC | 4 cut(s) | 121, 553, 675, 758 |
| MlsI | TGGCCA | 2 cut(s) | 893, 1684 |
| MluCI | AATT | 7 cut(s) | 217, 349, 768, 1041, 1060, 1724, 1764 |
| MluNI | TGGCCA | 2 cut(s) | 893, 1684 |
| Mly113I | GGCGCC | 2 cut(s) | 962, 2118 |
| MlyI | GAGTC | 2 cut(s) | 596, 1845 |
| MmeI | TCCRAC | 2 cut(s) | 1824, 2008 |
| Mox20I | TGGCCA | 2 cut(s) | 893, 1684 |
| Mph1103I | ATGCAT | 1 cut(s) | 1696 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2096 |
| MscI | TGGCCA | 2 cut(s) | 893, 1684 |
| MseI | TTAA | 6 cut(s) | 1074, 1398, 1847, 1913, 2072, 2209 |
| MslI | CAYNNNNRTG | 2 cut(s) | 146, 1156 |
| Msp20I | TGGCCA | 2 cut(s) | 893, 1684 |
| MspA1I | CMGCKG | 4 cut(s) | 462, 707, 1373, 2155 |
| MspI | CCGG | 7 cut(s) | 183, 250, 1140, 1218, 1472, 2057, 2121 |
| MspR9I | CCNGG | 8 cut(s) | 200, 250, 741, 1141, 1155, 1218, 1488, 1773 |
| MunI | CAATTG | 1 cut(s) | 1041 |
| Mva1269I | GAATGC | 1 cut(s) | 1696 |
| MvaI | CCWGG | 5 cut(s) | 200, 741, 1155, 1488, 1773 |
| MvnI | CGCG | 1 cut(s) | 1277 |
| NarI | GGCGCC | 2 cut(s) | 962, 2118 |
| NciI | CCSGG | 3 cut(s) | 250, 1141, 1218 |
| NcoI | CCATGG | 1 cut(s) | 888 |
| NdeI | CATATG | 1 cut(s) | 1342 |
| NlaIV | GGNNCC | 5 cut(s) | 963, 1240, 1262, 2119, 2125 |
| NmeAIII | GCCGAG | 1 cut(s) | 2126 |
| NmuCI | GTSAC | 4 cut(s) | 178, 734, 1963, 1981 |
| NsiI | ATGCAT | 1 cut(s) | 1696 |
| NspI | RCATGY | 3 cut(s) | 145, 659, 1553 |
| OliI | CACNNNNGTG | 1 cut(s) | 1156 |
| PagI | TCATGA | 2 cut(s) | 1456, 1668 |
| PciI | ACATGT | 1 cut(s) | 1549 |
| PciSI | GCTCTTC | 1 cut(s) | 977 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 382, 1719 |
| PctI | GAATGC | 1 cut(s) | 1696 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2096 |
| PfeI | GAWTC | 9 cut(s) | 240, 260, 319, 435, 446, 900, 997, 1498, 1535 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 889 |
| PfoI | TCCNGGA | 3 cut(s) | 198, 1216, 1771 |
| PleI | GAGTC | 2 cut(s) | 595, 1845 |
| PluTI | GGCGCC | 2 cut(s) | 965, 2121 |
| PpsI | GAGTC | 2 cut(s) | 595, 1845 |
| PscI | ACATGT | 1 cut(s) | 1549 |
| PsiI | TTATAA | 2 cut(s) | 222, 726 |
| Psp1406I | AACGTT | 1 cut(s) | 1625 |
| Psp6I | CCWGG | 5 cut(s) | 198, 739, 1153, 1486, 1771 |
| PspEI | GGTNACC | 1 cut(s) | 2104 |
| PspFI | CCCAGC | 1 cut(s) | 1206 |
| PspGI | CCWGG | 5 cut(s) | 198, 739, 1153, 1486, 1771 |
| PspN4I | GGNNCC | 5 cut(s) | 963, 1240, 1262, 2119, 2125 |
| PspPI | GGNCC | 7 cut(s) | 358, 476, 932, 1439, 1741, 2123, 2163 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1304 |
| PsuI | RGATCY | 1 cut(s) | 2030 |
| PvuII | CAGCTG | 3 cut(s) | 707, 1373, 2155 |
| RsaI | GTAC | 6 cut(s) | 379, 557, 847, 1088, 1559, 2084 |
| RsaNI | GTAC | 6 cut(s) | 378, 556, 846, 1087, 1558, 2083 |
| RseI | CAYNNNNRTG | 2 cut(s) | 146, 1156 |
| SapI | GCTCTTC | 1 cut(s) | 977 |
| SaqAI | TTAA | 6 cut(s) | 1074, 1398, 1847, 1913, 2072, 2209 |
| Sau96I | GGNCC | 7 cut(s) | 358, 476, 932, 1439, 1741, 2123, 2163 |
| SchI | GAGTC | 2 cut(s) | 596, 1845 |
| ScrFI | CCNGG | 8 cut(s) | 200, 250, 741, 1141, 1155, 1218, 1488, 1773 |
| SduI | GDGCHC | 4 cut(s) | 121, 553, 675, 758 |
| SfaNI | GCATC | 7 cut(s) | 635, 778, 826, 1112, 1117, 1256, 1703 |
| SfoI | GGCGCC | 2 cut(s) | 963, 2119 |
| SinI | GGWCC | 4 cut(s) | 476, 932, 2123, 2163 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 146, 1156 |
| SmlI | CTYRAG | 3 cut(s) | 362, 1227, 1935 |
| SmoI | CTYRAG | 3 cut(s) | 362, 1227, 1935 |
| SpeI | ACTAGT | 2 cut(s) | 161, 1777 |
| Sse9I | AATT | 7 cut(s) | 217, 349, 768, 1041, 1060, 1724, 1764 |
| SspDI | GGCGCC | 2 cut(s) | 961, 2117 |
| SspI | AATATT | 1 cut(s) | 1330 |
| StyD4I | CCNGG | 8 cut(s) | 198, 248, 739, 1139, 1153, 1216, 1486, 1771 |
| StyI | CCWWGG | 2 cut(s) | 888, 894 |
| TaiI | ACGT | 7 cut(s) | 344, 379, 943, 1177, 1599, 1628, 1992 |
| TaqI | TCGA | 3 cut(s) | 194, 1749, 1925 |
| TasI | AATT | 7 cut(s) | 217, 349, 768, 1041, 1060, 1724, 1764 |
| TatI | WGTACW | 1 cut(s) | 1557 |
| TauI | GCSGC | 7 cut(s) | 465, 516, 638, 779, 1280, 1613, 1824 |
| TfiI | GAWTC | 9 cut(s) | 240, 260, 319, 435, 446, 900, 997, 1498, 1535 |
| Tru1I | TTAA | 6 cut(s) | 1074, 1398, 1847, 1913, 2072, 2209 |
| Tru9I | TTAA | 6 cut(s) | 1074, 1398, 1847, 1913, 2072, 2209 |
| TscAI | CASTG | 4 cut(s) | 436, 913, 982, 1840 |
| TseFI | GTSAC | 4 cut(s) | 178, 734, 1963, 1981 |
| TseI | GCWGC | 7 cut(s) | 245, 707, 983, 1370, 1505, 1760, 1869 |
| Tsp45I | GTSAC | 4 cut(s) | 178, 734, 1963, 1981 |
| TspDTI | ATGAA | 8 cut(s) | 343, 997, 1010, 1284, 1445, 1703, 1757, 1800 |
| TspGWI | ACGGA | 6 cut(s) | 252, 1092, 1116, 1695, 1749, 1961 |
| TspRI | CASTG | 4 cut(s) | 436, 913, 982, 1840 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 889 |
| VneI | GTGCAC | 2 cut(s) | 117, 754 |
| VpaK11BI | GGWCC | 4 cut(s) | 476, 932, 2123, 2163 |
| XapI | RAATTY | 4 cut(s) | 349, 768, 1060, 1724 |
| XbaI | TCTAGA | 1 cut(s) | 1162 |
| XceI | RCATGY | 3 cut(s) | 145, 659, 1553 |
| XmiI | GTMKAC | 3 cut(s) | 108, 1098, 1996 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2096 |
| Zsp2I | ATGCAT | 1 cut(s) | 1696 |