Prupe.6G067600_v2.0.a1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000257
Physical Location & Seq
Reverse (-)
4652706 .. 4657147
4442 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G067600.1

Sequence Viewer

Length: 2220 bp
ATGGCTAAGCATAGAGCTCTTCTGTTTTATCATGCTTCCATAATTCTCATCCTCACTCTGAGTTTATTTTGCAAAGCAACTTCTGAGGATAGAAAGGTGTATATTGTGTACTTGGGATCACTTCCTGATTGGGTTTTCTCACCATCTTCTCACCACCTTAGTATTCTACAAAGAGTTGTCCAGGAAAATGGTTCTGCTGAAAATTTGTTGGTAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAAGCTCACTGACCAGGAAAGAGAAAAACTTTCTAATATGAAGGAAGTGGTCTCTGTCTTTCCAAGCAGACCATTGCAACTTCAAACAACAAGATCTTGGGACTTTATGGGTTTCAATGAGAAAGTCAAGCGAAATGCCGGTGTTGAGGGAGATATTATTGTTGGTGTGATTGACAGTGGAATTTGGCCGGAGTCAGATAGCTTTAAAGATGAAGGTTTTGGTCCTCCTCCAAAGAAGTGGAAAGGTGCTTGTGAAGGTGGCAAAAATTTCACTTGCAACAACAAGGTCATTGGAGCACGATATTACTTAACAGAGTCAGCAAGGGATGAATGGGGTCATGGAACCCACACTGCCTCAACAGCAGCAGGGAATGCTGTAAAGGATGTAAGCTTTTATGGACTAGCACAAGGAACTGCAAGAGGAGGTGTTCCCGCTGCAAGAATTGCAGTGTATAAAGTGTGCTCCCGTGAAAAATCGTGCAGTGCACACAATACCTTGGGTGCTTTTGACGATGCTATTGCTGATGGAGTTGACATTATTACAATTTCAGTTTCATTTACTGCCATAAGAGATTTCGACGAGGACCCTATTGCAATTGGTGCTTTTCACGCAATGGAGAAAGGGATACTAACATCAAACTCTGCCGGCAACAATGGTCCTTCAGGTGCTACTGTGTCAAGTGTAGCTCCATGGATGCTTACAGTTGGAGCAAGTAGCATGGACCGTCGGATTATTGACAAGGTTGTTCTTGGAGATGGAAGGACACTAGTTGGAAACTCTGTCAACACTTTCGGATTAAATGGAACAAGCTTTCCTCTGATACATGGCAAAGATGTTTCAAGGAACTGCACAGAAAAAAGTGCCGGAAGTTGTGAACAAGGTTGCTTGGACAGTGATTTGGTGAAGGGGAAGATTGTGCTATGTGATCGATACACTAGTGGAGTTTCTGAGGCCTATAAAGTTGGTGCACTGGGTTCAATTTTAACCAATTACATCAATATTGATGATGCTTCTTTTGTTCTCCCATTACCTGCGTCAACTTTAAACAACGCAGAGTATAATGAGGTCATGTCCTACATGAACTCCACCAGAGATCCTCAAGCAAACATATTAAAAAGTGAAGTCATAAAAGATCTGGTTGCACCTATTGTTGCCTCCTTGTCTGCTCGTGGACCGAATCTCATTTTACCCGATATTATCAAGCCAGATATAAGTGCCCCGGGGGTAGAAATTTTGGCTGCATATTCACCTAATGCTTCTATCTCAATTCCGCAAGACATGAGGCGTGTTAAATACAACATAATGACTGGAACCTCCATGGCTTGCCCCCACGCCACTGCTGTAGCTGCATATGTTAAAACCTTCCACCCTGATTGGTCTCCTGCAGCCATCAAATCATCTATTATGACTACTGCTTGGCCCATGAATGAGACCAACAGCAACATTTCCAGTGGTGAATTTGCTTATGGATCCGGACATATCAATCCTGTAAAAGCAATAGACCCCGGGCTTGTGTATGAAGTGTTTAAAGAAGACTACGTAAATTTGCTATGCATGAAATATGAAGAAAGCATGGTGAGGCTTATATCAGGAGACAACAGCAGTTGCCCTACAGGTTCTGAAAAAGGATCTCCAGTGGATCTTAATTACCCTTCAATGGGAGCTAAAGTTGCAGCAGTGGAACCTTTTACGGTAAAATTTCACAGAACAGTTAAAAATGTTGGCCTTGCAAACTCCACTTACAAGGCCATTATGTCTCCTAATGCCAAAGTTGACATCAAGGTGGTGCCAGAAGTTATTTCCTTCGCGTCCTTGAACGAGGAGAAGACTTTTGACGTAACAGTTGCTGGAAGTGGTTTGCCATATGAATTGCGAGTTTCTGCTTCACTGGTTTGGTCTGATGGTACTCATAGTGTTAGAAGTCCAATTGTTGTGTACACAGTACCTAAAATAATGAGTAAAGAGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

740

Amino Acids

79.66

Weight (kDa)

6.56

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1288
AccB1I GGYRCC 1 cut(s) 2035
AccII CGCG 1 cut(s) 2057
AccIII TCCGGA 1 cut(s) 1721
AciI CCGC 2 cut(s) 681, 1520
AclWI GGATC 6 cut(s) 124, 1337, 1713, 1726, 1885, 1896
AcoI YGGCCR 1 cut(s) 434
AcsI RAATTY 7 cut(s) 202, 429, 514, 1479, 1706, 1792, 1946
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 4 cut(s) 110, 2155, 2186, 2193
AfiI CCNNNNNNNGG 2 cut(s) 1906, 1907
AgsI TTSAA 7 cut(s) 235, 332, 364, 1089, 1227, 1905, 2065
AhlI ACTAGT 2 cut(s) 1015, 1184
AjnI CCWGG 2 cut(s) 180, 261
AloI GAACNNNNNNTCC 2 cut(s) 1045, 1077
AluBI AGCT 8 cut(s) 17, 253, 450, 639, 935, 1059, 1595, 1913
AluI AGCT 8 cut(s) 17, 253, 450, 639, 935, 1059, 1595, 1913
Alw21I GWGCWC 5 cut(s) 19, 547, 713, 736, 1219
Alw26I GTCTC 6 cut(s) 304, 1632, 1673, 1836, 2010, 2207
Alw44I GTGCAC 2 cut(s) 732, 1215
AlwI GGATC 6 cut(s) 124, 1337, 1713, 1726, 1885, 1896
AlwNI CAGNNNCTG 2 cut(s) 1868, 2096
Ama87I CYCGRG 2 cut(s) 1468, 1754
Aor13HI TCCGGA 1 cut(s) 1721
AoxI GGCC 5 cut(s) 434, 1200, 1667, 1972, 1995
ApaLI GTGCAC 2 cut(s) 732, 1215
ApeKI GCWGC 7 cut(s) 245, 611, 683, 1487, 1595, 1634, 1922
ApoI RAATTY 7 cut(s) 202, 429, 514, 1479, 1706, 1792, 1946
AspS9I GGNCC 6 cut(s) 470, 832, 905, 970, 1421, 1668
AsuC2I CCSGG 4 cut(s) 1469, 1470, 1755, 1756
AsuHPI GGTGA 6 cut(s) 132, 143, 1162, 1488, 1715, 1837
AvaI CYCGRG 2 cut(s) 1468, 1754
AvaII GGWCC 5 cut(s) 470, 832, 905, 970, 1421
BaeGI GKGCMC 3 cut(s) 736, 1219, 1468
BaeI ACNNNNGTAYC 2 cut(s) 1171, 1204
BamHI GGATCC 1 cut(s) 1718
BanI GGYRCC 1 cut(s) 2035
BanII GRGCYC 1 cut(s) 19
BauI CACGAG 1 cut(s) 1416
BbsI GAAGAC 2 cut(s) 1788, 2081
Bbv12I GWGCWC 5 cut(s) 19, 547, 713, 736, 1219
BbvI GCAGC 7 cut(s) 232, 623, 670, 1474, 1582, 1646, 1934
BccI CCATC 5 cut(s) 151, 767, 998, 1646, 2144
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 2 cut(s) 182, 263
BciVI GTATCC 1 cut(s) 867
BcnI CCSGG 4 cut(s) 1469, 1470, 1755, 1756
BcoDI GTCTC 6 cut(s) 304, 1632, 1673, 1836, 2010, 2207
BcuI ACTAGT 2 cut(s) 1015, 1184
BfaI CTAG 3 cut(s) 650, 1016, 1185
BfmI CTRYAG 3 cut(s) 1590, 1632, 1860
BfuAI ACCTGC 1 cut(s) 1288
BfuI GTATCC 1 cut(s) 867
BglII AGATCT 2 cut(s) 341, 1381
BisI GCNGC 7 cut(s) 246, 612, 684, 1488, 1596, 1635, 1923
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 7 cut(s) 247, 613, 685, 1489, 1597, 1636, 1924
Bme1390I CCNGG 6 cut(s) 182, 263, 1469, 1470, 1755, 1756
Bme18I GGWCC 5 cut(s) 470, 832, 905, 970, 1421
BmeT110I CYCGRG 2 cut(s) 1468, 1754
BmgT120I GGNCC 6 cut(s) 470, 832, 905, 970, 1421, 1668
BmiI GGNNCC 6 cut(s) 592, 834, 1561, 1720, 1932, 2037
BmrFI CCNGG 6 cut(s) 182, 263, 1469, 1470, 1755, 1756
BmrI ACTGGG 1 cut(s) 1229
BmsI GCATC 3 cut(s) 751, 933, 1246
BmuI ACTGGG 1 cut(s) 1229
BpiI GAAGAC 2 cut(s) 1788, 2081
BpmI CTGGAG 1 cut(s) 1866
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 1332
BpuMI CCSGG 4 cut(s) 1469, 1470, 1755, 1756
Bsa29I ATCGAT 1 cut(s) 1177
BsaAI YACGTR 1 cut(s) 1789
BsaI GGTCTC 4 cut(s) 304, 1632, 1673, 2207
BsaJI CCNNGG 8 cut(s) 744, 938, 1467, 1468, 1469, 1566, 1753, 1754
BsaWI WCCGGW 1 cut(s) 1721
BsaXI ACNNNNNCTCC 4 cut(s) 1316, 1346, 1902, 1932
Bsc4I CCNNNNNNNGG 2 cut(s) 1906, 1907
Bse118I RCCGGY 2 cut(s) 386, 893
Bse1I ACTGG 5 cut(s) 1224, 1561, 1698, 1883, 2142
Bse3DI GCAATG 2 cut(s) 320, 867
BseAI TCCGGA 1 cut(s) 1721
BseBI CCWGG 2 cut(s) 182, 263
BseCI ATCGAT 1 cut(s) 1177
BseDI CCNNGG 8 cut(s) 744, 938, 1467, 1468, 1469, 1566, 1753, 1754
BseGI GGATG 4 cut(s) 48, 580, 637, 948
BseLI CCNNNNNNNGG 2 cut(s) 1906, 1907
BseMI GCAATG 2 cut(s) 320, 867
BseMII CTCAG 3 cut(s) 50, 75, 1188
BseNI ACTGG 5 cut(s) 1224, 1561, 1698, 1883, 2142
BseRI GAGGAG 3 cut(s) 465, 684, 2084
BseSI GKGCMC 3 cut(s) 736, 1219, 1468
BseXI GCAGC 7 cut(s) 232, 623, 670, 1474, 1582, 1646, 1934
BsgI GTGCAG 2 cut(s) 748, 1081
Bsh1236I CGCG 1 cut(s) 2057
BshFI GGCC 5 cut(s) 436, 1202, 1669, 1974, 1997
BshNI GGYRCC 1 cut(s) 2035
BshVI ATCGAT 1 cut(s) 1177
BsiHKAI GWGCWC 5 cut(s) 19, 547, 713, 736, 1219
BsiHKCI CYCGRG 2 cut(s) 1468, 1754
BsiSI CCGG 7 cut(s) 387, 437, 894, 1113, 1469, 1722, 1755
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 1906, 1907
BsmAI GTCTC 6 cut(s) 304, 1632, 1673, 1836, 2010, 2207
BsmFI GGGAC 1 cut(s) 362
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 5 cut(s) 436, 1202, 1669, 1974, 1997
Bso31I GGTCTC 4 cut(s) 304, 1632, 1673, 2207
BsoBI CYCGRG 2 cut(s) 1468, 1754
Bsp1286I GDGCHC 6 cut(s) 19, 547, 713, 736, 1219, 1468
Bsp13I TCCGGA 1 cut(s) 1721
Bsp1407I TGTACA 1 cut(s) 2184
Bsp143I GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 938, 1566
BspACI CCGC 2 cut(s) 681, 1520
BspANI GGCC 5 cut(s) 436, 1202, 1669, 1974, 1997
BspCNI CTCAG 3 cut(s) 51, 76, 1189
BspDI ATCGAT 1 cut(s) 1177
BspEI TCCGGA 1 cut(s) 1721
BspFNI CGCG 1 cut(s) 2057
BspLI GGNNCC 6 cut(s) 592, 834, 1561, 1720, 1932, 2037
BspMAI CTGCAG 1 cut(s) 1636
BspMI ACCTGC 1 cut(s) 1288
BspPI GGATC 6 cut(s) 124, 1337, 1713, 1726, 1885, 1896
BspQI GCTCTTC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 2035
BspTNI GGTCTC 4 cut(s) 304, 1632, 1673, 2207
BsrDI GCAATG 2 cut(s) 320, 867
BsrFI RCCGGY 2 cut(s) 386, 893
BsrGI TGTACA 1 cut(s) 2184
BsrI ACTGG 5 cut(s) 1224, 1561, 1698, 1883, 2142
BssAI RCCGGY 2 cut(s) 386, 893
BssECI CCNNGG 8 cut(s) 744, 938, 1467, 1468, 1469, 1566, 1753, 1754
BssMI GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
BssSI CACGAG 1 cut(s) 1416
BssT1I CCWWGG 3 cut(s) 744, 938, 1566
Bst2BI CACGAG 1 cut(s) 1416
Bst2UI CCWGG 2 cut(s) 182, 263
Bst4CI ACNGT 9 cut(s) 425, 922, 952, 974, 1142, 1942, 1960, 2092, 2191
Bst6I CTCTTC 1 cut(s) 24
BstAPI GCANNNNNTGC 3 cut(s) 620, 692, 848
BstAUI TGTACA 1 cut(s) 2184
BstBAI YACGTR 1 cut(s) 1789
BstC8I GCNNGC 2 cut(s) 895, 1573
BstDEI CTNAG 5 cut(s) 6, 59, 84, 158, 1197
BstDSI CCRYGG 2 cut(s) 938, 1566
BstF5I GGATG 4 cut(s) 48, 580, 637, 948
BstFNI CGCG 1 cut(s) 2057
BstKTI GATC 8 cut(s) 119, 344, 1177, 1345, 1384, 1721, 1880, 1891
BstMAI GTCTC 6 cut(s) 304, 1632, 1673, 1836, 2010, 2207
BstMBI GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
BstMWI GCNNNNNNNGC 7 cut(s) 608, 620, 692, 848, 857, 1595, 1919
BstNI CCWGG 2 cut(s) 182, 263
BstSCI CCNGG 6 cut(s) 180, 261, 1467, 1468, 1753, 1754
BstSFI CTRYAG 3 cut(s) 1590, 1632, 1860
BstSLI GKGCMC 3 cut(s) 736, 1219, 1468
BstSNI TACGTA 1 cut(s) 1789
BstUI CGCG 1 cut(s) 2057
BstV1I GCAGC 7 cut(s) 232, 623, 670, 1474, 1582, 1646, 1934
BstV2I GAAGAC 2 cut(s) 1788, 2081
BstX2I RGATCY 6 cut(s) 341, 1342, 1381, 1718, 1877, 1888
BstXI CCANNNNNNTGG 2 cut(s) 188, 486
BstYI RGATCY 6 cut(s) 341, 1342, 1381, 1718, 1877, 1888
Bsu15I ATCGAT 1 cut(s) 1177
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 5 cut(s) 436, 1202, 1669, 1974, 1997
BsuTUI ATCGAT 1 cut(s) 1177
BtgI CCRYGG 2 cut(s) 938, 1566
BtsCI GGATG 4 cut(s) 48, 580, 637, 948
BtsI GCAGTG 5 cut(s) 597, 702, 736, 1584, 1932
BveI ACCTGC 1 cut(s) 1288
Cac8I GCNNGC 2 cut(s) 895, 1573
CaiI CAGNNNCTG 2 cut(s) 1868, 2096
Cfr10I RCCGGY 2 cut(s) 386, 893
Cfr13I GGNCC 6 cut(s) 470, 832, 905, 970, 1421, 1668
Cfr9I CCCGGG 2 cut(s) 1468, 1754
ClaI ATCGAT 1 cut(s) 1177
CseI GACGC 2 cut(s) 1272, 2046
Csp6I GTAC 4 cut(s) 109, 2154, 2185, 2192
CspCI CAANNNNNGTGG 2 cut(s) 1604, 1639
CviQI GTAC 4 cut(s) 109, 2154, 2185, 2192
DdeI CTNAG 5 cut(s) 6, 59, 84, 158, 1197
DpnI GATC 8 cut(s) 118, 343, 1176, 1344, 1383, 1720, 1879, 1890
DpnII GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
DraI TTTAAA 3 cut(s) 454, 1293, 1777
EaeI YGGCCR 1 cut(s) 434
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 17
Eco105I TACGTA 1 cut(s) 1789
Eco130I CCWWGG 3 cut(s) 744, 938, 1566
Eco147I AGGCCT 1 cut(s) 1202
Eco24I GRGCYC 1 cut(s) 19
Eco31I GGTCTC 4 cut(s) 304, 1632, 1673, 2207
Eco47I GGWCC 5 cut(s) 470, 832, 905, 970, 1421
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 1468, 1754
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 832
EcoRII CCWGG 2 cut(s) 180, 261
EcoT14I CCWWGG 3 cut(s) 744, 938, 1566
EcoT22I ATGCAT 1 cut(s) 1805
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 3 cut(s) 744, 938, 1566
FaqI GGGAC 1 cut(s) 362
FauI CCCGC 1 cut(s) 688
FauNDI CATATG 2 cut(s) 1600, 2113
Fnu4HI GCNGC 7 cut(s) 246, 612, 684, 1488, 1596, 1635, 1923
FokI GGATG 4 cut(s) 35, 587, 644, 955
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 7 cut(s) 246, 612, 684, 1488, 1596, 1635, 1923
FspBI CTAG 3 cut(s) 650, 1016, 1185
GluI GCNGC 7 cut(s) 246, 612, 684, 1488, 1596, 1635, 1923
GsuI CTGGAG 1 cut(s) 1866
HaeIII GGCC 5 cut(s) 436, 1202, 1669, 1974, 1997
HapII CCGG 7 cut(s) 387, 437, 894, 1113, 1469, 1722, 1755
HgaI GACGC 2 cut(s) 1272, 2046
HincII GTYRAC 4 cut(s) 781, 1033, 1287, 2023
HindII GTYRAC 4 cut(s) 781, 1033, 1287, 2023
HindIII AAGCTT 2 cut(s) 637, 1057
HinfI GANTC 3 cut(s) 440, 563, 1426
HpaII CCGG 7 cut(s) 387, 437, 894, 1113, 1469, 1722, 1755
HphI GGTGA 6 cut(s) 132, 143, 1162, 1488, 1715, 1837
Hpy188I TCNGA 9 cut(s) 60, 85, 445, 978, 1043, 1068, 1198, 1870, 2149
Hpy188III TCNNGA 3 cut(s) 125, 1722, 1839
Hpy99I CGWCG 2 cut(s) 830, 978
HpyAV CCTTC 9 cut(s) 283, 455, 497, 918, 1002, 1147, 1621, 1911, 2062
HpyCH4III ACNGT 9 cut(s) 425, 922, 952, 974, 1142, 1942, 1960, 2092, 2191
HpyCH4IV ACGT 2 cut(s) 1788, 2085
HpyF10VI GCNNNNNNNGC 7 cut(s) 608, 620, 692, 848, 857, 1595, 1919
HpyF3I CTNAG 5 cut(s) 6, 59, 84, 158, 1197
HpySE526I ACGT 2 cut(s) 1788, 2085
Kpn2I TCCGGA 1 cut(s) 1721
KroI GCCGGC 1 cut(s) 893
KroNI GCCGGC 1 cut(s) 895
Kzo9I GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 5 cut(s) 542, 716, 940, 956, 1910
Lsp1109I GCAGC 7 cut(s) 232, 623, 670, 1474, 1582, 1646, 1934
LweI GCATC 3 cut(s) 751, 933, 1246
MaeI CTAG 3 cut(s) 650, 1016, 1185
MaeII ACGT 2 cut(s) 1788, 2085
MaeIII GTNAC 2 cut(s) 218, 2086
MalI GATC 8 cut(s) 118, 343, 1176, 1344, 1383, 1720, 1879, 1890
MboI GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
MboII GAAGA 6 cut(s) 11, 138, 1171, 1793, 1826, 2086
MfeI CAATTG 2 cut(s) 843, 2175
MflI RGATCY 6 cut(s) 341, 1342, 1381, 1718, 1877, 1888
MhlI GDGCHC 6 cut(s) 19, 547, 713, 736, 1219, 1468
MlyI GAGTC 2 cut(s) 449, 572
MmeI TCCRAC 3 cut(s) 934, 956, 1000
Mph1103I ATGCAT 1 cut(s) 1805
MroI TCCGGA 1 cut(s) 1721
MroNI GCCGGC 1 cut(s) 893
MslI CAYNNNNRTG 1 cut(s) 2030
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 7 cut(s) 387, 437, 894, 1113, 1469, 1722, 1755
MspR9I CCNGG 6 cut(s) 182, 263, 1469, 1470, 1755, 1756
MunI CAATTG 2 cut(s) 843, 2175
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 2 cut(s) 182, 263
MvnI CGCG 1 cut(s) 2057
MwoI GCNNNNNNNGC 7 cut(s) 608, 620, 692, 848, 857, 1595, 1919
NaeI GCCGGC 1 cut(s) 895
NciI CCSGG 4 cut(s) 1469, 1470, 1755, 1756
NcoI CCATGG 2 cut(s) 938, 1566
NdeI CATATG 2 cut(s) 1600, 2113
NdeII GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
NgoMIV GCCGGC 1 cut(s) 893
NlaIV GGNNCC 6 cut(s) 592, 834, 1561, 1720, 1932, 2037
NsiI ATGCAT 1 cut(s) 1805
PceI AGGCCT 1 cut(s) 1202
PciSI GCTCTTC 1 cut(s) 24
PctI GAATGC 1 cut(s) 625
PdiI GCCGGC 1 cut(s) 895
PfeI GAWTC 1 cut(s) 1426
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 7 cut(s) 247, 613, 685, 1489, 1597, 1636, 1924
PleI GAGTC 2 cut(s) 448, 571
PpsI GAGTC 2 cut(s) 448, 571
Ppu21I YACGTR 1 cut(s) 1789
PpuMI RGGWCCY 1 cut(s) 832
Psp124BI GAGCTC 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 2 cut(s) 180, 261
PspGI CCWGG 2 cut(s) 180, 261
PspN4I GGNNCC 6 cut(s) 592, 834, 1561, 1720, 1932, 2037
PspPI GGNCC 6 cut(s) 470, 832, 905, 970, 1421, 1668
PspPPI RGGWCCY 1 cut(s) 832
PstI CTGCAG 1 cut(s) 1636
PstNI CAGNNNCTG 2 cut(s) 1868, 2096
PsuI RGATCY 6 cut(s) 341, 1342, 1381, 1718, 1877, 1888
RsaI GTAC 4 cut(s) 110, 2155, 2186, 2193
RsaNI GTAC 4 cut(s) 109, 2154, 2185, 2192
RseI CAYNNNNRTG 1 cut(s) 2030
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 24
SatI GCNGC 7 cut(s) 246, 612, 684, 1488, 1596, 1635, 1923
Sau3AI GATC 8 cut(s) 116, 341, 1174, 1342, 1381, 1718, 1877, 1888
Sau96I GGNCC 6 cut(s) 470, 832, 905, 970, 1421, 1668
SchI GAGTC 2 cut(s) 449, 572
ScrFI CCNGG 6 cut(s) 182, 263, 1469, 1470, 1755, 1756
SduI GDGCHC 6 cut(s) 19, 547, 713, 736, 1219, 1468
SfaNI GCATC 3 cut(s) 751, 933, 1246
SfcI CTRYAG 3 cut(s) 1590, 1632, 1860
SinI GGWCC 5 cut(s) 470, 832, 905, 970, 1421
SmaI CCCGGG 2 cut(s) 1470, 1756
SmiMI CAYNNNNRTG 1 cut(s) 2030
SmlI CTYRAG 1 cut(s) 1347
SmoI CTYRAG 1 cut(s) 1347
SnaBI TACGTA 1 cut(s) 1789
SpeI ACTAGT 2 cut(s) 1015, 1184
SseBI AGGCCT 1 cut(s) 1202
SsiI CCGC 2 cut(s) 681, 1520
SspI AATATT 1 cut(s) 1249
SspMI CTAG 3 cut(s) 650, 1016, 1185
SstI GAGCTC 1 cut(s) 19
StuI AGGCCT 1 cut(s) 1202
StyD4I CCNGG 6 cut(s) 180, 261, 1467, 1468, 1753, 1754
StyI CCWWGG 3 cut(s) 744, 938, 1566
TaaI ACNGT 9 cut(s) 425, 922, 952, 974, 1142, 1942, 1960, 2092, 2191
TaiI ACGT 2 cut(s) 1791, 2088
TaqI TCGA 2 cut(s) 825, 1177
TaqII GACCGA 1 cut(s) 1438
TatI WGTACW 2 cut(s) 108, 2184
TfiI GAWTC 1 cut(s) 1426
TseI GCWGC 7 cut(s) 245, 611, 683, 1487, 1595, 1634, 1922
TspMI CCCGGG 2 cut(s) 1468, 1754
VneI GTGCAC 2 cut(s) 732, 1215
VpaK11BI GGWCC 5 cut(s) 470, 832, 905, 970, 1421
XapI RAATTY 7 cut(s) 202, 429, 514, 1479, 1706, 1792, 1946
XmaI CCCGGG 2 cut(s) 1468, 1754
XspI CTAG 3 cut(s) 650, 1016, 1185
Zsp2I ATGCAT 1 cut(s) 1805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.