RchiOBHm_Chr5g0066891

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000257
Physical Location & Seq
Forward (+)
72946682 .. 72950104
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34264

Sequence Viewer

Length: 2256 bp
ATGACAAAGCATGGAGCTCTTCTGTTTTATCACACCTTGTCAATTCTCATCCTCACTCTGAGTTTGTTATGCAGAGCCACTTGCGACGATAGAAAGGTCTATATTGTGTACTTGGGGTCACTTTCTGATGGTTTGTACTCGCCGTCATCTCACCATCTTAGTATTCTACAGAGAGTTGTCCAGGACAGTTCTCCTGAAAATGTTTTGGTAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAGACTCACTGACCAGGAAAGAGAAAAACTTTCTAAAATGAAGGAAGTAGTCTCTGTTTTTCCAAGCAGAACATTGCAACTTCAAACAACAAGATCTTGGGACTTCATGGGATTCAATGAGAAAATTGGTCGAAATGCCACAGTTGAGAGTGATATTATTGTGGGTGTCATTGACAGTGGAATTTGGCCTGAATCTGAGAGTTTTAAGGATGAAGGTTTTGGTCCTCCACCCAAGAAGTGGAAAGGTGCTTGTGAAGGGGGCAAAAAATTTACTTGCAACAACAAGCTTATTGGAGCTCGGTATTACTCGTCAACAGAGTCTGCAAGGGATGACACAGGTCACGGAACCCACACTGCCTCAACGGCAGCAGGAAATGCTGTAAGAGATGTAAGCTTTTATGGACTAGCACAAGGTACGGCAAGAGGAGGAGTTCCCTCTGCAAGAATTGCAGCATACAAAGTCTGTACCCCGGGCACATTGTGTAGTGCACATGATACCCTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTCGATATTATTACAGCTTCAGTTGCATTTAGTATGATACAAGAATTCGATGTGGATCCTCTTGCAATTGGTACTTTTCACGCAATGGAGAAAGGGATACTAACCTCACATGCTGCGGGCAACAATGGACCTTCCGGTGCTACTGTAACAAGTGTAGCACCTTGGGTGCTTACAGTTGCAGCAAGTAGCATGGACCGTCGGATCATTGACAAGGTTATCCTTGGAAATGGAAGGACAGTAATTGGGAATTCTGTGAACTCCTTCACATTAAATGGTACAAGTTTTCCTCTGATACATGGAAAAAATGCTTCAAAGAATTGCACAGAAACAATTGCGGGAGGTTGTGAACAAGGTTGCCTGGACAGTAGTCTAGTGAAGGGAAAGGTTGTGCTATGTGATAAATATGGACTTCTTGAAGCATTTCAATCTGGTGCACTAGGTTCAATTTTACCCAATTTTCGAGCTGTTGATGATGCCTCTTATGTTCTCCCATTAGCTGGCACGACTATAAACAACGAAAACTATAATGTAGTCATGTCATACTTGAACTCCACCAGATATCCTCGCGTGAACATATTAAAAAGTGAAGTCATAAAAAATCCTGCTGCACCTGCTGTTGCTTCATTCTCTGCACGTGGACCGAATTTAATTTTACCTGATATTATCAAGCCAGATATAAGTGCCCCAGGGATAGAAATTCTGGCTGCATTTTCGTCTGCTCCTATCTCAACCACTCCTGGAGATATGAGGCGTGTTAAGTACAATATACAATCTGGAACCTCCATGGCTTGCCCCCATGTTGCTGGTGTAGCTACCTATGTTAAAACCTTCCGCCCTGATTGGTCTCCCGCGGCCATCAAATCATCTATTATGACTACTGCAGCTATTTCTTATACACATTTTCATAATCAATCTACCAAATCTTTTTGGCTTGTAGCTTGGCCCATGAATGATCCCAGCAGCAATGTTTCCACTGGTGAATTCGCTTATGGATCAGGCCATATCAATCCATTAAAAGCTATTGACCCCGGGCTTGTGTATGATGCTTCCAAAGAAGATTACATAAAGTTGCTGTGCATCAGCTATGATGAAAGCAGAGTTAGACTTGTATCAGGAGATAACAGCACATGCCCTACAGGTCCTGACAAGGGATCTCCGAAGGATCATAATTACCCTTCGATGGGAGCAAAAGTTACCGCAATGCAACCATTTACAGTTAAATTTAATAGAAGAGTTAAAAATGTTGGCAATGCAAACTCCACTTATGTAGCCAAAATCTCCCCCAATTCCAAACTCGAGATTAAAGTGGTGCCGGAAGTCCTATCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTAATGTGACTGTTTCTGGAAGCAATTTGCCTTTTGAATCACGAGCATCTGCTTCACTGGTTTGGTCTGATGGTACTCATGGCGTTAGAAGTCCGATTGTTGTGTACACCGTGCCTAGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

751

Amino Acids

80.66

Weight (kDa)

8.74

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 110 1.8e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 132 - 562 1.3e-41 Subtilase family
fn3_6 PF17766 647 - 745 2e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1396
Acc36I ACCTGC 1 cut(s) 1396
AccB1I GGYRCC 1 cut(s) 2086
AccB7I CCANNNNNTGG 2 cut(s) 483, 1274
AccII CGCG 2 cut(s) 1344, 1628
AciI CCGC 6 cut(s) 893, 1112, 1609, 1626, 1628, 1974
AclWI GGATC 7 cut(s) 827, 840, 986, 1724, 1777, 1936, 1947
AcoI YGGCCR 1 cut(s) 1629
AcsI RAATTY 8 cut(s) 426, 512, 821, 1024, 1420, 1473, 1757, 1997
AcuI CTGAAG 1 cut(s) 780
AcvI CACGTG 1 cut(s) 1412
AdeI CACNNNGTG 1 cut(s) 726
AfaI GTAC 9 cut(s) 110, 137, 661, 712, 850, 1054, 1538, 2206, 2237
AfiI CCNNNNNNNGG 5 cut(s) 483, 1274, 1925, 1957, 1958
AjnI CCWGG 6 cut(s) 180, 258, 744, 1134, 1462, 1513
Alw21I GWGCWC 4 cut(s) 19, 544, 736, 1213
Alw26I GTCTC 2 cut(s) 301, 1626
Alw44I GTGCAC 2 cut(s) 732, 1209
AlwI GGATC 7 cut(s) 827, 840, 986, 1724, 1777, 1936, 1947
AlwNI CAGNNNCTG 2 cut(s) 566, 1919
Ama87I CYCGRG 3 cut(s) 715, 1805, 2072
AoxI GGCC 4 cut(s) 431, 1629, 1718, 1774
ApaLI GTGCAC 2 cut(s) 732, 1209
ApoI RAATTY 8 cut(s) 426, 512, 821, 1024, 1420, 1473, 1757, 1997
Asp700I GAANNNNTTC 2 cut(s) 1037, 1197
AspS9I GGNCC 6 cut(s) 467, 905, 970, 1415, 1719, 1916
AsuC2I CCSGG 4 cut(s) 716, 717, 1806, 1807
AsuHPI GGTGA 2 cut(s) 143, 1766
AvaI CYCGRG 3 cut(s) 715, 1805, 2072
AvaII GGWCC 5 cut(s) 467, 905, 970, 1415, 1916
BaeGI GKGCMC 4 cut(s) 722, 736, 1213, 1462
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 1 cut(s) 2086
BanII GRGCYC 2 cut(s) 19, 544
BauI CACGAG 1 cut(s) 2172
BbrPI CACGTG 1 cut(s) 1412
BbsI GAAGAC 1 cut(s) 2132
Bbv12I GWGCWC 4 cut(s) 19, 544, 736, 1213
BccI CCATC 6 cut(s) 122, 162, 767, 1640, 1951, 2195
BceAI ACGGC 3 cut(s) 127, 624, 678
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 6 cut(s) 182, 260, 746, 1136, 1464, 1515
BciVI GTATCC 1 cut(s) 867
BcnI CCSGG 4 cut(s) 716, 717, 1806, 1807
BcoDI GTCTC 2 cut(s) 301, 1626
BfaI CTAG 4 cut(s) 650, 1148, 1214, 2247
BfmI CTRYAG 3 cut(s) 167, 1656, 1911
BfuAI ACCTGC 1 cut(s) 1396
BfuI GTATCC 1 cut(s) 867
BglI GCCNNNNNGGC 1 cut(s) 608
BglII AGATCT 1 cut(s) 338
Bme18I GGWCC 5 cut(s) 467, 905, 970, 1415, 1916
BmeT110I CYCGRG 3 cut(s) 715, 1805, 2072
BmgT120I GGNCC 6 cut(s) 467, 905, 970, 1415, 1719, 1916
BmiI GGNNCC 4 cut(s) 592, 834, 1555, 2088
BmsI GCATC 5 cut(s) 751, 1240, 1810, 1863, 2186
BoxI GACNNNNGTC 2 cut(s) 582, 1143
BpiI GAAGAC 1 cut(s) 2132
BpmI CTGGAG 1 cut(s) 1536
BpuMI CCSGG 4 cut(s) 716, 717, 1806, 1807
BsaAI YACGTR 1 cut(s) 1412
BsaBI GATNNNNATC 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 1626
BsaWI WCCGGW 1 cut(s) 911
BsaXI ACNNNNNCTCC 4 cut(s) 1310, 1340, 1953, 1983
Bsc4I CCNNNNNNNGG 5 cut(s) 483, 1274, 1925, 1957, 1958
Bse1I ACTGG 2 cut(s) 1756, 2193
Bse3DI GCAATG 5 cut(s) 317, 867, 1747, 1983, 2032
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 6 cut(s) 182, 260, 746, 1136, 1464, 1515
BseGI GGATG 3 cut(s) 48, 460, 580
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 5 cut(s) 483, 1274, 1925, 1957, 1958
BseMI GCAATG 5 cut(s) 317, 867, 1747, 1983, 2032
BseMII CTCAG 2 cut(s) 50, 432
BseNI ACTGG 2 cut(s) 1756, 2193
BseRI GAGGAG 3 cut(s) 684, 687, 2135
BseSI GKGCMC 4 cut(s) 722, 736, 1213, 1462
BseYI CCCAGC 1 cut(s) 1733
BsgI GTGCAG 2 cut(s) 1368, 1392
Bsh1236I CGCG 2 cut(s) 1344, 1628
BshFI GGCC 4 cut(s) 433, 1631, 1720, 1776
BshNI GGYRCC 1 cut(s) 2086
BsiHKAI GWGCWC 4 cut(s) 19, 544, 736, 1213
BsiHKCI CYCGRG 3 cut(s) 715, 1805, 2072
BsiSI CCGG 4 cut(s) 716, 912, 1806, 2090
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 5 cut(s) 483, 1274, 1925, 1957, 1958
BsmAI GTCTC 2 cut(s) 301, 1626
BsmFI GGGAC 1 cut(s) 359
BsnI GGCC 4 cut(s) 433, 1631, 1720, 1776
Bso31I GGTCTC 1 cut(s) 1626
BsoBI CYCGRG 3 cut(s) 715, 1805, 2072
Bsp1286I GDGCHC 6 cut(s) 19, 544, 722, 736, 1213, 1462
Bsp1407I TGTACA 1 cut(s) 2235
Bsp143I GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
Bsp19I CCATGG 1 cut(s) 1560
BspACI CCGC 6 cut(s) 893, 1112, 1609, 1626, 1628, 1974
BspANI GGCC 4 cut(s) 433, 1631, 1720, 1776
BspCNI CTCAG 2 cut(s) 51, 433
BspFNI CGCG 2 cut(s) 1344, 1628
BspLI GGNNCC 4 cut(s) 592, 834, 1555, 2088
BspMAI CTGCAG 1 cut(s) 1660
BspMI ACCTGC 1 cut(s) 1396
BspPI GGATC 7 cut(s) 827, 840, 986, 1724, 1777, 1936, 1947
BspQI GCTCTTC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 2086
BspTNI GGTCTC 1 cut(s) 1626
BsrDI GCAATG 5 cut(s) 317, 867, 1747, 1983, 2032
BsrGI TGTACA 1 cut(s) 2235
BsrI ACTGG 2 cut(s) 1756, 2193
BssMI GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
BssSI CACGAG 1 cut(s) 2172
BssT1I CCWWGG 3 cut(s) 938, 997, 1560
Bst2BI CACGAG 1 cut(s) 2172
Bst2UI CCWGG 6 cut(s) 182, 260, 746, 1136, 1464, 1515
Bst6I CTCTTC 2 cut(s) 24, 2002
BstAPI GCANNNNNTGC 2 cut(s) 620, 692
BstAUI TGTACA 1 cut(s) 2235
BstBAI YACGTR 1 cut(s) 1412
BstC8I GCNNGC 3 cut(s) 895, 1276, 1567
BstDEI CTNAG 3 cut(s) 59, 158, 441
BstDSI CCRYGG 2 cut(s) 1560, 1626
BstF5I GGATG 3 cut(s) 48, 460, 580
BstFNI CGCG 2 cut(s) 1344, 1628
BstKTI GATC 7 cut(s) 341, 835, 981, 1732, 1772, 1931, 1942
BstMAI GTCTC 2 cut(s) 301, 1626
BstMBI GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
BstMWI GCNNNNNNNGC 6 cut(s) 608, 620, 692, 800, 1388, 1586
BstNI CCWGG 6 cut(s) 182, 260, 746, 1136, 1464, 1515
BstNSI RCATGY 2 cut(s) 890, 1908
BstPAI GACNNNNGTC 2 cut(s) 582, 1143
BstSFI CTRYAG 3 cut(s) 167, 1656, 1911
BstSLI GKGCMC 4 cut(s) 722, 736, 1213, 1462
BstUI CGCG 2 cut(s) 1344, 1628
BstV2I GAAGAC 1 cut(s) 2132
BstX2I RGATCY 3 cut(s) 338, 832, 1928
BstXI CCANNNNNNTGG 1 cut(s) 1580
BstYI RGATCY 3 cut(s) 338, 832, 1928
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 4 cut(s) 433, 1631, 1720, 1776
BtgI CCRYGG 2 cut(s) 1560, 1626
BtsCI GGATG 3 cut(s) 48, 460, 580
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 5 cut(s) 252, 427, 597, 1749, 2186
BveI ACCTGC 1 cut(s) 1396
Cac8I GCNNGC 3 cut(s) 895, 1276, 1567
CaiI CAGNNNCTG 2 cut(s) 566, 1919
Cfr13I GGNCC 6 cut(s) 467, 905, 970, 1415, 1719, 1916
Cfr42I CCGCGG 1 cut(s) 1629
Cfr9I CCCGGG 2 cut(s) 715, 1805
Csp6I GTAC 9 cut(s) 109, 136, 660, 711, 849, 1053, 1537, 2205, 2236
CviQI GTAC 9 cut(s) 109, 136, 660, 711, 849, 1053, 1537, 2205, 2236
DdeI CTNAG 3 cut(s) 59, 158, 441
DpnI GATC 7 cut(s) 340, 834, 980, 1731, 1771, 1930, 1941
DpnII GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
DraIII CACNNNGTG 1 cut(s) 726
EaeI YGGCCR 1 cut(s) 1629
Eam1104I CTCTTC 2 cut(s) 24, 2002
EarI CTCTTC 2 cut(s) 24, 2002
EciI GGCGGA 1 cut(s) 1598
Ecl136II GAGCTC 2 cut(s) 17, 542
Eco130I CCWWGG 3 cut(s) 938, 997, 1560
Eco24I GRGCYC 2 cut(s) 19, 544
Eco31I GGTCTC 1 cut(s) 1626
Eco32I GATATC 1 cut(s) 1337
Eco47I GGWCC 5 cut(s) 467, 905, 970, 1415, 1916
Eco53kI GAGCTC 2 cut(s) 17, 542
Eco57I CTGAAG 1 cut(s) 780
Eco72I CACGTG 1 cut(s) 1412
Eco88I CYCGRG 3 cut(s) 715, 1805, 2072
EcoICRI GAGCTC 2 cut(s) 17, 542
EcoO109I RGGNCCY 1 cut(s) 1916
EcoRI GAATTC 3 cut(s) 821, 1024, 1757
EcoRII CCWGG 6 cut(s) 180, 258, 744, 1134, 1462, 1513
EcoRV GATATC 1 cut(s) 1337
EcoT14I CCWWGG 3 cut(s) 938, 997, 1560
EcoT38I GRGCYC 2 cut(s) 19, 544
ErhI CCWWGG 3 cut(s) 938, 997, 1560
FaqI GGGAC 1 cut(s) 359
FauI CCCGC 3 cut(s) 886, 1105, 1633
FokI GGATG 3 cut(s) 35, 467, 587
FriOI GRGCYC 2 cut(s) 19, 544
FspBI CTAG 4 cut(s) 650, 1148, 1214, 2247
GsaI CCCAGC 1 cut(s) 1737
GsuI CTGGAG 1 cut(s) 1536
HaeIII GGCC 4 cut(s) 433, 1631, 1720, 1776
HapII CCGG 4 cut(s) 716, 912, 1806, 2090
HincII GTYRAC 1 cut(s) 558
HindII GTYRAC 1 cut(s) 558
HindIII AAGCTT 2 cut(s) 530, 637
HinfI GANTC 6 cut(s) 249, 357, 437, 563, 777, 2168
HpaII CCGG 4 cut(s) 716, 912, 1806, 2090
HphI GGTGA 2 cut(s) 143, 1766
Hpy188I TCNGA 8 cut(s) 60, 127, 442, 978, 1068, 1935, 2200, 2226
Hpy188III TCNNGA 8 cut(s) 194, 1190, 1551, 1890, 1919, 2074, 2148, 2172
Hpy99I CGWCG 2 cut(s) 89, 978
HpyCH4IV ACGT 1 cut(s) 1411
HpyF10VI GCNNNNNNNGC 6 cut(s) 608, 620, 692, 800, 1388, 1586
HpyF3I CTNAG 3 cut(s) 59, 158, 441
HpySE526I ACGT 1 cut(s) 1411
KspI CCGCGG 1 cut(s) 1629
Kzo9I GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 4 cut(s) 14, 539, 1501, 1961
LweI GCATC 5 cut(s) 751, 1240, 1810, 1863, 2186
MaeI CTAG 4 cut(s) 650, 1148, 1214, 2247
MaeII ACGT 1 cut(s) 1411
MaeIII GTNAC 6 cut(s) 117, 215, 584, 922, 1969, 2137
MalI GATC 7 cut(s) 340, 834, 980, 1731, 1771, 1930, 1941
MboI GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
MboII GAAGA 4 cut(s) 11, 1844, 2019, 2137
MfeI CAATTG 3 cut(s) 765, 843, 1107
MflI RGATCY 3 cut(s) 338, 832, 1928
MhlI GDGCHC 6 cut(s) 19, 544, 722, 736, 1213, 1462
MlyI GAGTC 3 cut(s) 243, 572, 786
MmeI TCCRAC 1 cut(s) 956
MroXI GAANNNNTTC 2 cut(s) 1037, 1197
MspA1I CMGCKG 1 cut(s) 1628
MspI CCGG 4 cut(s) 716, 912, 1806, 2090
MunI CAATTG 3 cut(s) 765, 843, 1107
MvaI CCWGG 6 cut(s) 182, 260, 746, 1136, 1464, 1515
MvnI CGCG 2 cut(s) 1344, 1628
MwoI GCNNNNNNNGC 6 cut(s) 608, 620, 692, 800, 1388, 1586
NciI CCSGG 4 cut(s) 716, 717, 1806, 1807
NcoI CCATGG 1 cut(s) 1560
NdeII GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
NlaIV GGNNCC 4 cut(s) 592, 834, 1555, 2088
NmuCI GTSAC 3 cut(s) 117, 584, 2137
NspI RCATGY 2 cut(s) 890, 1908
PaeR7I CTCGAG 1 cut(s) 2072
PaqCI CACCTGC 1 cut(s) 1396
PasI CCCWGGG 1 cut(s) 1463
PciSI GCTCTTC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 1037, 1197
PfeI GAWTC 3 cut(s) 357, 437, 2168
PflMI CCANNNNNTGG 2 cut(s) 483, 1274
PfoI TCCNGGA 2 cut(s) 180, 1513
PleI GAGTC 3 cut(s) 243, 571, 785
PmaCI CACGTG 1 cut(s) 1412
PmlI CACGTG 1 cut(s) 1412
PpsI GAGTC 3 cut(s) 243, 571, 785
Ppu21I YACGTR 1 cut(s) 1412
PpuMI RGGWCCY 1 cut(s) 1916
PshAI GACNNNNGTC 2 cut(s) 582, 1143
Psp124BI GAGCTC 2 cut(s) 19, 544
Psp5II RGGWCCY 1 cut(s) 1916
Psp6I CCWGG 6 cut(s) 180, 258, 744, 1134, 1462, 1513
PspCI CACGTG 1 cut(s) 1412
PspFI CCCAGC 1 cut(s) 1733
PspGI CCWGG 6 cut(s) 180, 258, 744, 1134, 1462, 1513
PspN4I GGNNCC 4 cut(s) 592, 834, 1555, 2088
PspPI GGNCC 6 cut(s) 467, 905, 970, 1415, 1719, 1916
PspPPI RGGWCCY 1 cut(s) 1916
PstI CTGCAG 1 cut(s) 1660
PstNI CAGNNNCTG 2 cut(s) 566, 1919
PsuI RGATCY 3 cut(s) 338, 832, 1928
RsaI GTAC 9 cut(s) 110, 137, 661, 712, 850, 1054, 1538, 2206, 2237
RsaNI GTAC 9 cut(s) 109, 136, 660, 711, 849, 1053, 1537, 2205, 2236
SacI GAGCTC 2 cut(s) 19, 544
SacII CCGCGG 1 cut(s) 1629
SapI GCTCTTC 1 cut(s) 24
Sau3AI GATC 7 cut(s) 338, 832, 978, 1729, 1769, 1928, 1939
Sau96I GGNCC 6 cut(s) 467, 905, 970, 1415, 1719, 1916
SchI GAGTC 3 cut(s) 243, 572, 786
SduI GDGCHC 6 cut(s) 19, 544, 722, 736, 1213, 1462
SfaNI GCATC 5 cut(s) 751, 1240, 1810, 1863, 2186
SfcI CTRYAG 3 cut(s) 167, 1656, 1911
Sfr274I CTCGAG 1 cut(s) 2072
Sfr303I CCGCGG 1 cut(s) 1629
SgrBI CCGCGG 1 cut(s) 1629
SinI GGWCC 5 cut(s) 467, 905, 970, 1415, 1916
SlaI CTCGAG 1 cut(s) 2072
SmaI CCCGGG 2 cut(s) 717, 1807
SmlI CTYRAG 1 cut(s) 2072
SmoI CTYRAG 1 cut(s) 2072
SsiI CCGC 6 cut(s) 893, 1112, 1609, 1626, 1628, 1974
SspMI CTAG 4 cut(s) 650, 1148, 1214, 2247
SstI GAGCTC 2 cut(s) 19, 544
StyI CCWWGG 3 cut(s) 938, 997, 1560
TaiI ACGT 1 cut(s) 1414
TaqI TCGA 6 cut(s) 376, 780, 825, 1237, 1955, 2073
TaqII GACCGA 1 cut(s) 1432
TatI WGTACW 4 cut(s) 108, 135, 1536, 2235
TauI GCSGC 1 cut(s) 1631
TfiI GAWTC 3 cut(s) 357, 437, 2168
TscAI CASTG 5 cut(s) 259, 427, 604, 1756, 2193
TseFI GTSAC 3 cut(s) 117, 584, 2137
Tsp45I GTSAC 3 cut(s) 117, 584, 2137
TspDTI ATGAA 7 cut(s) 299, 340, 471, 1389, 1670, 1739, 1881
TspGWI ACGGA 1 cut(s) 603
TspMI CCCGGG 2 cut(s) 715, 1805
TspRI CASTG 5 cut(s) 259, 427, 604, 1756, 2193
Van91I CCANNNNNTGG 2 cut(s) 483, 1274
VneI GTGCAC 2 cut(s) 732, 1209
VpaK11BI GGWCC 5 cut(s) 467, 905, 970, 1415, 1916
XapI RAATTY 8 cut(s) 426, 512, 821, 1024, 1420, 1473, 1757, 1997
XceI RCATGY 2 cut(s) 890, 1908
XcmI CCANNNNNNNNNTGG 1 cut(s) 480
XhoI CTCGAG 1 cut(s) 2072
XmaI CCCGGG 2 cut(s) 715, 1805
XmnI GAANNNNTTC 2 cut(s) 1037, 1197
XspI CTAG 4 cut(s) 650, 1148, 1214, 2247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.