FvH4_1g22560
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000261
Physical Location & Seq
Forward (+)
14471428 .. 14472934
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22560.t1

Sequence Viewer

Length: 1239 bp
ATGGATGGAAGAAATCTGTTGTTGATCTTGCTGTTGGAACTGTGGCATTTAGAGAATATGTCTGTTTGTACAATCCTTGTGTTATTGATGTTGAAATTGAGGCAAAGACGAGATCGCCGAATTGCTTTAGATAATCGATCATTTGTTAGATTTGAGACTCGGTTGATTTATCTAGATAGTATAATAGGCAGTAGTGACACCGAGTGTGTGAATGAGTTAAGAATGGATAGGAGGACTTTTGGGTTGTTATGTGAACTGCTCCGCACTGATGGAAGGCTAAAAAATGATGGATTAGTTAGTGTGGAGGAGCAAGTTTGTATGTTTCTACACATACTTGCTCATCATGTTAAAAATCGTACCATTAGAGGTAGATTCTTCCGATCAGGAGAGACAGTTAGTAGGTATTTCAACTGTGTATTGCAAGGTGTGTTACGATTACAAGCCAATCTATTAAGGATGTCAGACCCCGTACTTGATAATTGCACAGATAATAGATGGAAATGGTTTAAGAATTGCTTGAGAGCTTTAGATGGAACTTATGTTAGAGTACGTGTAGCTACTACTGACAAGCCAAGATACCGAACTAGAAAGGCAGATATTGCAACAAATGTCTTGGCAGCTTGTTCGCGTGACATGCAGTTCACATTCGTGTTACCGGGATGGGAGGGTTCTGCATCCGACTCTAGAAAGTGCATTGAAGTTGATAGCAATGATGCATGGGATATGTATGTTCAGCACAACAAAAATGCCAAAGGTTGGAGAAATAAAAGTTTTCCAATTTATGATCATCTTGCTCTGATTTTTGGCAAGGATCGTGCCAATGGAAGAGGAGCTGAAACCCCTGCTGATATGGCGGATGATCAAAGCATTAATGATATTAATTCTGAGCAAATGGTTAATGATGTAAGTCCTGTGTCACTTAACCAAGATTCTACCCAAACTGAAGGTAGTAGGAAGAGGAAGAGAGTTGATGAGACTGATAAACTTGTTGTTGCTTTGGACAAAGTATTTGAAGAATCAGGAAAGAGGATGCAAATGGTAACTGAGGCCATATTGAAGGGTAATGAAGATCGCTCTGACATTGCCAAAGAGTTGAAGAACATAGGATTATCTGTTGATGATCAAATTGTTGCATTAGGAATCATCCTAGAGAGACCACAGAACATCTCTATCTTCAAGTCATTGGATGATGATGTTAGAAGGGTATATGTTCAAAACTTGCTTATGAGCAATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

413

Amino Acids

47.45

Weight (kDa)

8.66

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 57 - 144 3.8e-24 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 176 - 276 3.6e-06 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18411 FvH4_1g22560 FvH4_1g24290 FvH4_1g24290 FvH4_1g24290 FvH4_1g29434 FvH4_2g04031 FvH4_2g04811 FvH4_2g07610 FvH4_2g09412 FvH4_3g15552 FvH4_3g30720 FvH4_4g05810 FvH4_4g09541 FvH4_4g15171 FvH4_6g09145 FvH4_6g49541 FvH4_7g00320 FvH4_7g11552
malus_domestica MD00G1072400.v1.1 MD01G1188100.v1.1 MD07G1132000.v1.1 MD08G1190700.v1.1 MD10G1011200.v1.1 MD14G1164100.v1.1 MD15G1289200.v1.1 MD15G1289300.v1.1 MD17G1245100.v1.1
prunus_persica Prupe.6G228600_v2.0.a1
pyrus_communis pycom01g07070 pycom02g06890 pycom02g06900 pycom02g13930 pycom04g07470 pycom04g11770 pycom04g21930 pycom05g08520 pycom06g04490 pycom06g09560 pycom07g03750 pycom08g08760 pycom08g13660 pycom09g11520 pycom09g14250 pycom09g14770 pycom10g00720 pycom10g01290 pycom10g10480 pycom10g15700 pycom12420g00130 pycom12g05380 pycom12g08750 pycom13g19010 pycom13g29080 pycom14g06210 pycom14g13680 pycom14g19850 pycom15g22070 pycom15g23440 pycom15g24950 pycom15g25280 pycom16g11100 pycom16g17080 pycom16g26310 pycom17g15650 pycom17g15670 pycom17g20370
rosa_chinensis RchiOBHm_Chr1g0373591 RchiOBHm_Chr2g0105551 RchiOBHm_Chr2g0106711 RchiOBHm_Chr2g0106721 RchiOBHm_Chr7g0219611
rosa_laevigata RLG00000013773 RLG00000017436 RLG00000017533
rosa_multiflora Rmu_co8001842.1_g000001 Rmu_co8158450.1_g000001 Rmu_sc0000516.1_g000067 Rmu_sc0000566.1_g000009 Rmu_sc0000965.1_g000025 Rmu_sc0001718.1_g000001 Rmu_sc0001719.1_g000020 Rmu_sc0001822.1_g000025 Rmu_sc0002219.1_g000003 Rmu_sc0002460.1_g000059 Rmu_sc0002757.1_g000001 Rmu_sc0002860.1_g000001 Rmu_sc0004531.1_g000001 Rmu_sc0005558.1_g000003 Rmu_sc0011218.1_g000001 Rmu_sc0011998.1_g000003 Rmu_sc0015560.1_g000002 Rmu_sc0015560.1_g000003
rosa_roxburghii Rroxscaffold_1G00004200 Rroxscaffold_1G00026570 Rroxscaffold_1G00040370 Rroxscaffold_1G00044120 Rroxscaffold_1G00049590 Rroxscaffold_1G00059030 Rroxscaffold_2G00108440 Rroxscaffold_2G00108850 Rroxscaffold_2G00130680 Rroxscaffold_2G00132980 Rroxscaffold_2G00136860 Rroxscaffold_3G00218610 Rroxscaffold_4G00309940 Rroxscaffold_6G00405560
rosa_rugosa Rorug02G0138000 Rorug02G0138100 Rorug02G0138100 Rorug06G0154000
rosa_samantha Rh1AG121500 Rh1CG116300 Rh2AG189000 Rh2AG509600 Rh2BG200200 Rh2CG194100 Rh2DG195300 Rh5AG114400 Rh5DG488800
rosa_wichuraiana Rw0G022020 Rw1G023650 Rw1G026380 Rw1G034750 Rw1G040770 Rw2G014870 Rw2G016560 Rw4G031190 Rw5G026570 Rw6G003020 Rw6G012060 Rw7G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 756
AccII CGCG 1 cut(s) 628
AciI CCGC 2 cut(s) 262, 854
AclWI GGATC 1 cut(s) 819
AcuI CTGAAG 1 cut(s) 963
AdeI CACNNNGTG 1 cut(s) 204
AfaI GTAC 4 cut(s) 70, 358, 471, 549
AfiI CCNNNNNNNGG 1 cut(s) 756
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 8 cut(s) 94, 409, 698, 1013, 1057, 1096, 1177, 1214
AleI CACNNNNGTG 1 cut(s) 647
AluBI AGCT 4 cut(s) 524, 557, 620, 833
AluI AGCT 4 cut(s) 524, 557, 620, 833
Alw26I GTCTC 4 cut(s) 149, 383, 968, 1147
AlwI GGATC 1 cut(s) 819
AoxI GGCC 1 cut(s) 1047
ApeKI GCWGC 1 cut(s) 617
ArsI GACNNNNNNTTYG 2 cut(s) 992, 1024
AseI ATTAAT 2 cut(s) 870, 879
AsuC2I CCSGG 1 cut(s) 657
BbvI GCAGC 1 cut(s) 629
BccI CCATC 5 cut(s) 263, 281, 489, 524, 654
BcgI CGANNNNNNTGC 2 cut(s) 606, 640
BclI TGATCA 3 cut(s) 784, 859, 1120
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 4 cut(s) 149, 383, 968, 1147
BfaI CTAG 4 cut(s) 173, 585, 684, 1148
BisI GCNGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 657
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 3 cut(s) 683, 703, 1020
BpuEI CTTGAG 1 cut(s) 538
BpuMI CCSGG 1 cut(s) 657
Bsa29I ATCGAT 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 1147
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 756
Bse3DI GCAATG 2 cut(s) 715, 1080
BseCI ATCGAT 1 cut(s) 136
BseGI GGATG 8 cut(s) 10, 462, 665, 674, 862, 1035, 1143, 1192
BseLI CCNNNNNNNGG 1 cut(s) 756
BseMI GCAATG 2 cut(s) 715, 1080
BseMII CTCAG 2 cut(s) 876, 1035
BseRI GAGGAG 2 cut(s) 320, 843
BseXI GCAGC 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 628
BshFI GGCC 1 cut(s) 1049
BshVI ATCGAT 1 cut(s) 136
BsiSI CCGG 1 cut(s) 656
BslI CCNNNNNNNGG 1 cut(s) 756
BsmAI GTCTC 4 cut(s) 149, 383, 968, 1147
BsnI GGCC 1 cut(s) 1049
Bso31I GGTCTC 1 cut(s) 1147
Bsp1407I TGTACA 1 cut(s) 68
Bsp143I GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
BspACI CCGC 2 cut(s) 262, 854
BspANI GGCC 1 cut(s) 1049
BspCNI CTCAG 2 cut(s) 877, 1036
BspDI ATCGAT 1 cut(s) 136
BspFNI CGCG 1 cut(s) 628
BspPI GGATC 1 cut(s) 819
BspTNI GGTCTC 1 cut(s) 1147
BsrDI GCAATG 2 cut(s) 715, 1080
BsrGI TGTACA 1 cut(s) 68
BssMI GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
Bst4CI ACNGT 3 cut(s) 42, 394, 413
Bst6I CTCTTC 3 cut(s) 820, 950, 956
BstAPI GCANNNNNTGC 1 cut(s) 599
BstAUI TGTACA 1 cut(s) 68
BstBAI YACGTR 1 cut(s) 551
BstDEI CTNAG 2 cut(s) 885, 1044
BstF5I GGATG 8 cut(s) 10, 462, 665, 674, 862, 1035, 1143, 1192
BstFNI CGCG 1 cut(s) 628
BstKTI GATC 9 cut(s) 27, 115, 140, 383, 787, 814, 862, 1072, 1123
BstMAI GTCTC 4 cut(s) 149, 383, 968, 1147
BstMBI GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 599, 634, 851
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 1 cut(s) 655
BstUI CGCG 1 cut(s) 628
BstV1I GCAGC 1 cut(s) 629
Bsu15I ATCGAT 1 cut(s) 136
BsuRI GGCC 1 cut(s) 1049
BsuTUI ATCGAT 1 cut(s) 136
BtsCI GGATG 8 cut(s) 10, 462, 665, 674, 862, 1035, 1143, 1192
BtsIMutI CAGTG 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 136
Csp6I GTAC 4 cut(s) 69, 357, 470, 548
CviAII CATG 3 cut(s) 344, 634, 717
CviJI RGCY 8 cut(s) 277, 443, 524, 557, 571, 620, 833, 1049
CviKI_1 RGCY 8 cut(s) 277, 443, 524, 557, 571, 620, 833, 1049
CviQI GTAC 4 cut(s) 69, 357, 470, 548
DdeI CTNAG 2 cut(s) 885, 1044
DpnI GATC 9 cut(s) 26, 114, 139, 382, 786, 813, 861, 1071, 1122
DpnII GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
DraIII CACNNNGTG 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 820, 950, 956
EarI CTCTTC 3 cut(s) 820, 950, 956
EciI GGCGGA 1 cut(s) 869
Eco31I GGTCTC 1 cut(s) 1147
Eco57I CTGAAG 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 718
FaeI CATG 3 cut(s) 347, 637, 720
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FatI CATG 3 cut(s) 343, 633, 716
FbaI TGATCA 3 cut(s) 784, 859, 1120
Fnu4HI GCNGC 1 cut(s) 618
FokI GGATG 8 cut(s) 17, 469, 661, 672, 869, 1042, 1130, 1199
Fsp4HI GCNGC 1 cut(s) 618
FspBI CTAG 4 cut(s) 173, 585, 684, 1148
GluI GCNGC 1 cut(s) 618
HaeIII GGCC 1 cut(s) 1049
HapII CCGG 1 cut(s) 656
Hin1II CATG 3 cut(s) 347, 637, 720
HinfI GANTC 6 cut(s) 157, 372, 680, 929, 1016, 1140
HpaII CCGG 1 cut(s) 656
Hpy166II GTNNAC 2 cut(s) 254, 642
Hpy188I TCNGA 6 cut(s) 380, 463, 679, 798, 886, 1078
Hpy188III TCNNGA 4 cut(s) 173, 384, 684, 1020
Hpy8I GTNNAC 2 cut(s) 254, 642
HpyAV CCTTC 4 cut(s) 267, 938, 1051, 1194
HpyCH4III ACNGT 3 cut(s) 42, 394, 413
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 9 cut(s) 421, 483, 602, 637, 674, 693, 716, 1033, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 599, 634, 851
HpyF3I CTNAG 2 cut(s) 885, 1044
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 3 cut(s) 347, 637, 720
Ksp22I TGATCA 3 cut(s) 784, 859, 1120
Kzo9I GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
LmnI GCTCC 3 cut(s) 264, 307, 830
LpnPI CCDG 5 cut(s) 369, 669, 855, 924, 1005
Lsp1109I GCAGC 1 cut(s) 629
LweI GCATC 3 cut(s) 683, 703, 1020
MaeI CTAG 4 cut(s) 173, 585, 684, 1148
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 6 cut(s) 194, 429, 629, 651, 915, 1039
MalI GATC 9 cut(s) 26, 114, 139, 382, 786, 813, 861, 1071, 1122
MboI GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
MboII GAAGA 9 cut(s) 21, 367, 837, 967, 973, 1025, 1079, 1108, 1165
MluCI AATT 7 cut(s) 95, 120, 478, 511, 777, 880, 1125
MlyI GAGTC 2 cut(s) 151, 674
MmeI TCCRAC 3 cut(s) 15, 702, 737
MnlI CCTC 9 cut(s) 93, 225, 298, 359, 658, 821, 951, 1020, 1039
Mph1103I ATGCAT 1 cut(s) 718
MseI TTAA 8 cut(s) 218, 348, 452, 507, 870, 879, 897, 921
MslI CAYNNNNRTG 1 cut(s) 647
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 1 cut(s) 657
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 3 cut(s) 599, 634, 851
NciI CCSGG 1 cut(s) 657
NdeII GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
NlaIII CATG 3 cut(s) 347, 637, 720
NmuCI GTSAC 3 cut(s) 194, 629, 915
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 637
OliI CACNNNNGTG 1 cut(s) 647
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PfeI GAWTC 4 cut(s) 372, 929, 1016, 1140
PflMI CCANNNNNTGG 1 cut(s) 756
PkrI GCNGC 1 cut(s) 619
PleI GAGTC 2 cut(s) 151, 674
PpsI GAGTC 2 cut(s) 151, 674
Ppu21I YACGTR 1 cut(s) 551
PshBI ATTAAT 2 cut(s) 870, 879
RsaI GTAC 4 cut(s) 70, 358, 471, 549
RsaNI GTAC 4 cut(s) 69, 357, 470, 548
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 8 cut(s) 218, 348, 452, 507, 870, 879, 897, 921
SatI GCNGC 1 cut(s) 618
Sau3AI GATC 9 cut(s) 24, 112, 137, 380, 784, 811, 859, 1069, 1120
SchI GAGTC 2 cut(s) 151, 674
ScrFI CCNGG 1 cut(s) 657
SfaNI GCATC 3 cut(s) 683, 703, 1020
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 7 cut(s) 95, 120, 478, 511, 777, 880, 1125
SsiI CCGC 2 cut(s) 262, 854
SspMI CTAG 4 cut(s) 173, 585, 684, 1148
StyD4I CCNGG 1 cut(s) 655
TaaI ACNGT 3 cut(s) 42, 394, 413
TaiI ACGT 1 cut(s) 553
TaqI TCGA 1 cut(s) 136
TasI AATT 7 cut(s) 95, 120, 478, 511, 777, 880, 1125
TatI WGTACW 1 cut(s) 68
TfiI GAWTC 4 cut(s) 372, 929, 1016, 1140
Tru1I TTAA 8 cut(s) 218, 348, 452, 507, 870, 879, 897, 921
Tru9I TTAA 8 cut(s) 218, 348, 452, 507, 870, 879, 897, 921
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 3 cut(s) 194, 629, 915
TseI GCWGC 1 cut(s) 617
Tsp45I GTSAC 3 cut(s) 194, 629, 915
TspDTI ATGAA 1 cut(s) 1080
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 756
VspI ATTAAT 2 cut(s) 870, 879
XbaI TCTAGA 2 cut(s) 172, 683
XceI RCATGY 1 cut(s) 637
XspI CTAG 4 cut(s) 173, 585, 684, 1148
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.