MRG
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 156 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 274 |
| AccII | CGCG | 1 cut(s) | 319 |
| AciI | CCGC | 5 cut(s) | 734, 1016, 1115, 1574, 1637 |
| AclI | AACGTT | 1 cut(s) | 1543 |
| AclWI | GGATC | 2 cut(s) | 238, 1268 |
| AcoI | YGGCCR | 2 cut(s) | 1133, 1269 |
| AcsI | RAATTY | 6 cut(s) | 135, 286, 661, 799, 1171, 1193 |
| AcuI | CTGAAG | 2 cut(s) | 780, 1033 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 274, 620, 1124, 1332 |
| AgsI | TTSAA | 9 cut(s) | 47, 124, 823, 1161, 1176, 1465, 1478, 1549, 1651 |
| AjnI | CCWGG | 4 cut(s) | 295, 488, 1123, 1135 |
| Alw26I | GTCTC | 4 cut(s) | 333, 509, 1063, 1445 |
| AlwI | GGATC | 2 cut(s) | 238, 1268 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 422, 1720 |
| Ama87I | CYCGRG | 1 cut(s) | 502 |
| AoxI | GGCC | 3 cut(s) | 257, 1133, 1269 |
| ApoI | RAATTY | 6 cut(s) | 135, 286, 661, 799, 1171, 1193 |
| ArsI | GACNNNNNNTTYG | 1 cut(s) | 1739 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 64 |
| AspLEI | GCGC | 2 cut(s) | 157, 1344 |
| AspS9I | GGNCC | 4 cut(s) | 100, 302, 637, 1537 |
| AsuC2I | CCSGG | 2 cut(s) | 248, 969 |
| AsuHPI | GGTGA | 8 cut(s) | 17, 524, 758, 1095, 1106, 1113, 1217, 1626 |
| AvaI | CYCGRG | 1 cut(s) | 502 |
| AvaII | GGWCC | 4 cut(s) | 100, 302, 637, 1537 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1328, 1360 |
| BbvCI | CCTCAGC | 1 cut(s) | 1713 |
| BccI | CCATC | 4 cut(s) | 382, 403, 484, 664 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 713, 747 |
| BciT130I | CCWGG | 4 cut(s) | 297, 490, 1125, 1137 |
| BciVI | GTATCC | 1 cut(s) | 186 |
| BcnI | CCSGG | 2 cut(s) | 248, 969 |
| BcoDI | GTCTC | 4 cut(s) | 333, 509, 1063, 1445 |
| BfaI | CTAG | 1 cut(s) | 705 |
| BfmI | CTRYAG | 2 cut(s) | 1379, 1721 |
| BfuI | GTATCC | 1 cut(s) | 186 |
| BglII | AGATCT | 1 cut(s) | 1564 |
| Bme1390I | CCNGG | 6 cut(s) | 248, 297, 490, 969, 1125, 1137 |
| Bme18I | GGWCC | 4 cut(s) | 100, 302, 637, 1537 |
| BmeT110I | CYCGRG | 1 cut(s) | 502 |
| BmgT120I | GGNCC | 4 cut(s) | 100, 302, 637, 1537 |
| BmiI | GGNNCC | 1 cut(s) | 1616 |
| BmrFI | CCNGG | 6 cut(s) | 248, 297, 490, 969, 1125, 1137 |
| BmrI | ACTGGG | 1 cut(s) | 177 |
| BmsI | GCATC | 6 cut(s) | 24, 439, 573, 805, 1329, 1498 |
| BmuI | ACTGGG | 1 cut(s) | 177 |
| BpmI | CTGGAG | 1 cut(s) | 1607 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1713 |
| BpuMI | CCSGG | 2 cut(s) | 248, 969 |
| BsaI | GGTCTC | 1 cut(s) | 1445 |
| BsaJI | CCNNGG | 3 cut(s) | 295, 489, 1062 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 274, 620, 1124, 1332 |
| Bse1I | ACTGG | 1 cut(s) | 172 |
| Bse3DI | GCAATG | 2 cut(s) | 606, 1272 |
| BseBI | CCWGG | 4 cut(s) | 297, 490, 1125, 1137 |
| BseDI | CCNNGG | 3 cut(s) | 295, 489, 1062 |
| BseGI | GGATG | 8 cut(s) | 216, 374, 395, 433, 454, 675, 781, 844 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 274, 620, 1124, 1332 |
| BseMI | GCAATG | 2 cut(s) | 606, 1272 |
| BseMII | CTCAG | 8 cut(s) | 254, 402, 698, 1456, 1516, 1658, 1727, 1743 |
| BseNI | ACTGG | 1 cut(s) | 172 |
| BseYI | CCCAGC | 1 cut(s) | 1151 |
| BsgI | GTGCAG | 2 cut(s) | 675, 1389 |
| Bsh1236I | CGCG | 1 cut(s) | 319 |
| BshFI | GGCC | 3 cut(s) | 259, 1135, 1271 |
| BsiHKCI | CYCGRG | 1 cut(s) | 502 |
| BsiSI | CCGG | 2 cut(s) | 248, 968 |
| BslFI | GGGAC | 4 cut(s) | 754, 847, 907, 984 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 274, 620, 1124, 1332 |
| BsmAI | GTCTC | 4 cut(s) | 333, 509, 1063, 1445 |
| BsmBI | CGTCTC | 1 cut(s) | 1063 |
| BsmFI | GGGAC | 4 cut(s) | 754, 847, 907, 984 |
| BsmI | GAATGC | 1 cut(s) | 1576 |
| BsnI | GGCC | 3 cut(s) | 259, 1135, 1271 |
| Bso31I | GGTCTC | 1 cut(s) | 1445 |
| BsoBI | CYCGRG | 1 cut(s) | 502 |
| Bsp143I | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| Bsp19I | CCATGG | 1 cut(s) | 1062 |
| Bsp68I | TCGCGA | 1 cut(s) | 319 |
| BspACI | CCGC | 5 cut(s) | 734, 1016, 1115, 1574, 1637 |
| BspANI | GGCC | 3 cut(s) | 259, 1135, 1271 |
| BspCNI | CTCAG | 9 cut(s) | 4, 253, 403, 697, 1455, 1515, 1657, 1726, 1744 |
| BspFNI | CGCG | 1 cut(s) | 319 |
| BspLI | GGNNCC | 1 cut(s) | 1616 |
| BspMAI | CTGCAG | 1 cut(s) | 1725 |
| BspPI | GGATC | 2 cut(s) | 238, 1268 |
| BspTNI | GGTCTC | 1 cut(s) | 1445 |
| BsrDI | GCAATG | 2 cut(s) | 606, 1272 |
| BsrI | ACTGG | 1 cut(s) | 172 |
| BssECI | CCNNGG | 3 cut(s) | 295, 489, 1062 |
| BssMI | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| BssT1I | CCWWGG | 1 cut(s) | 1062 |
| Bst2UI | CCWGG | 4 cut(s) | 297, 490, 1125, 1137 |
| Bst4CI | ACNGT | 4 cut(s) | 878, 1070, 1205, 1401 |
| Bst6I | CTCTTC | 1 cut(s) | 832 |
| BstC8I | GCNNGC | 6 cut(s) | 681, 721, 959, 1254, 1284, 1554 |
| BstDSI | CCRYGG | 1 cut(s) | 1062 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1122 |
| BstF5I | GGATG | 8 cut(s) | 216, 374, 395, 433, 454, 675, 781, 844 |
| BstFNI | CGCG | 1 cut(s) | 319 |
| BstHHI | GCGC | 2 cut(s) | 157, 1344 |
| BstKTI | GATC | 5 cut(s) | 188, 246, 323, 1276, 1567 |
| BstMAI | GTCTC | 4 cut(s) | 333, 509, 1063, 1445 |
| BstMBI | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| BstNI | CCWGG | 4 cut(s) | 297, 490, 1125, 1137 |
| BstNSI | RCATGY | 1 cut(s) | 961 |
| BstSCI | CCNGG | 6 cut(s) | 246, 295, 488, 967, 1123, 1135 |
| BstSFI | CTRYAG | 2 cut(s) | 1379, 1721 |
| BstUI | CGCG | 1 cut(s) | 319 |
| BstX2I | RGATCY | 2 cut(s) | 243, 1564 |
| BstYI | RGATCY | 2 cut(s) | 243, 1564 |
| BsuI | GTATCC | 1 cut(s) | 186 |
| BsuRI | GGCC | 3 cut(s) | 259, 1135, 1271 |
| BtgI | CCRYGG | 1 cut(s) | 1062 |
| BtgZI | GCGATG | 1 cut(s) | 462 |
| BtsCI | GGATG | 8 cut(s) | 216, 374, 395, 433, 454, 675, 781, 844 |
| BtsI | GCAGTG | 3 cut(s) | 356, 922, 943 |
| BtsIMutI | CAGTG | 7 cut(s) | 9, 356, 423, 922, 943, 1219, 1397 |
| BtuMI | TCGCGA | 1 cut(s) | 319 |
| Cac8I | GCNNGC | 6 cut(s) | 681, 721, 959, 1254, 1284, 1554 |
| CaiI | CAGNNNCTG | 2 cut(s) | 422, 1720 |
| CfoI | GCGC | 2 cut(s) | 157, 1344 |
| Cfr13I | GGNCC | 4 cut(s) | 100, 302, 637, 1537 |
| CseI | GACGC | 1 cut(s) | 1045 |
| CviAII | CATG | 4 cut(s) | 406, 958, 1063, 1597 |
| DpnI | GATC | 5 cut(s) | 187, 245, 322, 1275, 1566 |
| DpnII | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| EaeI | YGGCCR | 2 cut(s) | 1133, 1269 |
| Eam1104I | CTCTTC | 1 cut(s) | 832 |
| EarI | CTCTTC | 1 cut(s) | 832 |
| Eco130I | CCWWGG | 1 cut(s) | 1062 |
| Eco31I | GGTCTC | 1 cut(s) | 1445 |
| Eco47I | GGWCC | 4 cut(s) | 100, 302, 637, 1537 |
| Eco57I | CTGAAG | 2 cut(s) | 780, 1033 |
| Eco88I | CYCGRG | 1 cut(s) | 502 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1122 |
| EcoRI | GAATTC | 1 cut(s) | 1171 |
| EcoRII | CCWGG | 4 cut(s) | 295, 488, 1123, 1135 |
| EcoT14I | CCWWGG | 1 cut(s) | 1062 |
| EcoT22I | ATGCAT | 1 cut(s) | 963 |
| ErhI | CCWWGG | 1 cut(s) | 1062 |
| Esp3I | CGTCTC | 1 cut(s) | 1063 |
| FaeI | CATG | 4 cut(s) | 409, 961, 1066, 1600 |
| FaqI | GGGAC | 4 cut(s) | 754, 847, 907, 984 |
| FatI | CATG | 4 cut(s) | 405, 957, 1062, 1596 |
| FauI | CCCGC | 1 cut(s) | 1009 |
| FokI | GGATG | 8 cut(s) | 203, 361, 382, 440, 461, 682, 788, 851 |
| FspBI | CTAG | 1 cut(s) | 705 |
| FspI | TGCGCA | 1 cut(s) | 156 |
| GlaI | GCGC | 2 cut(s) | 156, 1343 |
| GsaI | CCCAGC | 1 cut(s) | 1155 |
| GsuI | CTGGAG | 1 cut(s) | 1607 |
| HaeIII | GGCC | 3 cut(s) | 259, 1135, 1271 |
| HapII | CCGG | 2 cut(s) | 248, 968 |
| HgaI | GACGC | 1 cut(s) | 1045 |
| HhaI | GCGC | 2 cut(s) | 157, 1344 |
| Hin1II | CATG | 4 cut(s) | 409, 961, 1066, 1600 |
| Hin6I | GCGC | 2 cut(s) | 155, 1342 |
| HinP1I | GCGC | 2 cut(s) | 155, 1342 |
| HindIII | AAGCTT | 2 cut(s) | 1250, 1550 |
| HpaII | CCGG | 2 cut(s) | 248, 968 |
| HphI | GGTGA | 8 cut(s) | 17, 524, 758, 1095, 1106, 1113, 1217, 1626 |
| Hpy166II | GTNNAC | 2 cut(s) | 281, 458 |
| Hpy188III | TCNNGA | 4 cut(s) | 318, 386, 502, 863 |
| Hpy8I | GTNNAC | 2 cut(s) | 281, 458 |
| HpyAV | CCTTC | 7 cut(s) | 349, 731, 755, 992, 1128, 1484, 1596 |
| HpyCH4III | ACNGT | 4 cut(s) | 878, 1070, 1205, 1401 |
| HpyCH4IV | ACGT | 1 cut(s) | 1543 |
| HpySE526I | ACGT | 1 cut(s) | 1543 |
| Hsp92II | CATG | 4 cut(s) | 409, 961, 1066, 1600 |
| HspAI | GCGC | 2 cut(s) | 155, 1342 |
| Kzo9I | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| LmnI | GCTCC | 2 cut(s) | 616, 1626 |
| LweI | GCATC | 6 cut(s) | 24, 439, 573, 805, 1329, 1498 |
| MaeI | CTAG | 1 cut(s) | 705 |
| MaeII | ACGT | 1 cut(s) | 1543 |
| MaeIII | GTNAC | 5 cut(s) | 5, 908, 1101, 1445, 1632 |
| MalI | GATC | 5 cut(s) | 187, 245, 322, 1275, 1566 |
| MboI | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| MboII | GAAGA | 5 cut(s) | 42, 57, 819, 1240, 1348 |
| MfeI | CAATTG | 1 cut(s) | 1161 |
| MflI | RGATCY | 2 cut(s) | 243, 1564 |
| MlyI | GAGTC | 5 cut(s) | 422, 1109, 1369, 1636, 1763 |
| MmeI | TCCRAC | 2 cut(s) | 1338, 1564 |
| Mph1103I | ATGCAT | 1 cut(s) | 963 |
| MroXI | GAANNNNTTC | 1 cut(s) | 64 |
| MseI | TTAA | 7 cut(s) | 264, 368, 675, 693, 942, 1005, 1602 |
| MslI | CAYNNNNRTG | 1 cut(s) | 404 |
| MspA1I | CMGCKG | 1 cut(s) | 1316 |
| MspI | CCGG | 2 cut(s) | 248, 968 |
| MspR9I | CCNGG | 6 cut(s) | 248, 297, 490, 969, 1125, 1137 |
| MunI | CAATTG | 1 cut(s) | 1161 |
| Mva1269I | GAATGC | 1 cut(s) | 1576 |
| MvaI | CCWGG | 4 cut(s) | 297, 490, 1125, 1137 |
| MvnI | CGCG | 1 cut(s) | 319 |
| NciI | CCSGG | 2 cut(s) | 248, 969 |
| NcoI | CCATGG | 1 cut(s) | 1062 |
| NdeII | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| NlaIII | CATG | 4 cut(s) | 409, 961, 1066, 1600 |
| NlaIV | GGNNCC | 1 cut(s) | 1616 |
| NmeAIII | GCCGAG | 2 cut(s) | 1111, 1282 |
| NmuCI | GTSAC | 4 cut(s) | 5, 908, 1101, 1632 |
| NruI | TCGCGA | 1 cut(s) | 319 |
| NsbI | TGCGCA | 1 cut(s) | 156 |
| NsiI | ATGCAT | 1 cut(s) | 963 |
| NspI | RCATGY | 1 cut(s) | 961 |
| PaeI | GCATGC | 1 cut(s) | 961 |
| PaeR7I | CTCGAG | 1 cut(s) | 502 |
| PctI | GAATGC | 1 cut(s) | 1576 |
| PdmI | GAANNNNTTC | 1 cut(s) | 64 |
| PfeI | GAWTC | 6 cut(s) | 353, 389, 701, 859, 1430, 1577 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 274 |
| PleI | GAGTC | 5 cut(s) | 421, 1108, 1369, 1636, 1762 |
| PpsI | GAGTC | 5 cut(s) | 421, 1108, 1369, 1636, 1762 |
| Psp1406I | AACGTT | 1 cut(s) | 1543 |
| Psp6I | CCWGG | 4 cut(s) | 295, 488, 1123, 1135 |
| PspFI | CCCAGC | 1 cut(s) | 1151 |
| PspGI | CCWGG | 4 cut(s) | 295, 488, 1123, 1135 |
| PspN4I | GGNNCC | 1 cut(s) | 1616 |
| PspPI | GGNCC | 4 cut(s) | 100, 302, 637, 1537 |
| PstI | CTGCAG | 1 cut(s) | 1725 |
| PstNI | CAGNNNCTG | 2 cut(s) | 422, 1720 |
| PsuI | RGATCY | 2 cut(s) | 243, 1564 |
| PvuII | CAGCTG | 1 cut(s) | 1316 |
| RruI | TCGCGA | 1 cut(s) | 319 |
| RseI | CAYNNNNRTG | 1 cut(s) | 404 |
| SaqAI | TTAA | 7 cut(s) | 264, 368, 675, 693, 942, 1005, 1602 |
| Sau3AI | GATC | 5 cut(s) | 185, 243, 320, 1273, 1564 |
| Sau96I | GGNCC | 4 cut(s) | 100, 302, 637, 1537 |
| SchI | GAGTC | 5 cut(s) | 422, 1109, 1369, 1636, 1763 |
| ScrFI | CCNGG | 6 cut(s) | 248, 297, 490, 969, 1125, 1137 |
| SfaNI | GCATC | 6 cut(s) | 24, 439, 573, 805, 1329, 1498 |
| SfcI | CTRYAG | 2 cut(s) | 1379, 1721 |
| Sfr274I | CTCGAG | 1 cut(s) | 502 |
| SinI | GGWCC | 4 cut(s) | 100, 302, 637, 1537 |
| SlaI | CTCGAG | 1 cut(s) | 502 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 404 |
| SmlI | CTYRAG | 1 cut(s) | 502 |
| SmoI | CTYRAG | 1 cut(s) | 502 |
| SphI | GCATGC | 1 cut(s) | 961 |
| SsiI | CCGC | 5 cut(s) | 734, 1016, 1115, 1574, 1637 |
| SspI | AATATT | 1 cut(s) | 979 |
| SspMI | CTAG | 1 cut(s) | 705 |
| StyD4I | CCNGG | 6 cut(s) | 246, 295, 488, 967, 1123, 1135 |
| StyI | CCWWGG | 1 cut(s) | 1062 |
| TaaI | ACNGT | 4 cut(s) | 878, 1070, 1205, 1401 |
| TaiI | ACGT | 1 cut(s) | 1546 |
| TaqI | TCGA | 2 cut(s) | 503, 1042 |
| TfiI | GAWTC | 6 cut(s) | 353, 389, 701, 859, 1430, 1577 |
| Tru1I | TTAA | 7 cut(s) | 264, 368, 675, 693, 942, 1005, 1602 |
| Tru9I | TTAA | 7 cut(s) | 264, 368, 675, 693, 942, 1005, 1602 |
| TscAI | CASTG | 7 cut(s) | 16, 363, 423, 922, 943, 1219, 1404 |
| TseFI | GTSAC | 4 cut(s) | 5, 908, 1101, 1632 |
| Tsp45I | GTSAC | 4 cut(s) | 5, 908, 1101, 1632 |
| TspDTI | ATGAA | 7 cut(s) | 57, 812, 939, 960, 1241, 1260, 1533 |
| TspGWI | ACGGA | 2 cut(s) | 851, 1348 |
| TspRI | CASTG | 7 cut(s) | 16, 363, 423, 922, 943, 1219, 1404 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 274 |
| VpaK11BI | GGWCC | 4 cut(s) | 100, 302, 637, 1537 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1122 |
| XapI | RAATTY | 6 cut(s) | 135, 286, 661, 799, 1171, 1193 |
| XceI | RCATGY | 1 cut(s) | 961 |
| XhoI | CTCGAG | 1 cut(s) | 502 |
| XmnI | GAANNNNTTC | 1 cut(s) | 64 |
| XspI | CTAG | 1 cut(s) | 705 |
| Zsp2I | ATGCAT | 1 cut(s) | 963 |