isoform X1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 23 |
| Acc36I | ACCTGC | 1 cut(s) | 469 |
| AccI | GTMKAC | 1 cut(s) | 587 |
| AcsI | RAATTY | 2 cut(s) | 182, 578 |
| AcuI | CTGAAG | 1 cut(s) | 30 |
| AfaI | GTAC | 2 cut(s) | 148, 459 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 194 |
| AflIII | ACRYGT | 2 cut(s) | 49, 214 |
| AgsI | TTSAA | 3 cut(s) | 139, 497, 667 |
| AjiI | CACGTC | 1 cut(s) | 50 |
| AjnI | CCWGG | 1 cut(s) | 142 |
| AluBI | AGCT | 1 cut(s) | 493 |
| AluI | AGCT | 1 cut(s) | 493 |
| ApeKI | GCWGC | 3 cut(s) | 449, 464, 656 |
| ApoI | RAATTY | 2 cut(s) | 182, 578 |
| AspS9I | GGNCC | 1 cut(s) | 430 |
| AvaII | GGWCC | 1 cut(s) | 430 |
| BauI | CACGAG | 1 cut(s) | 595 |
| BbvI | GCAGC | 3 cut(s) | 436, 451, 643 |
| BccI | CCATC | 3 cut(s) | 285, 365, 369 |
| BciT130I | CCWGG | 1 cut(s) | 144 |
| BfaI | CTAG | 1 cut(s) | 92 |
| BfuAI | ACCTGC | 1 cut(s) | 469 |
| BisI | GCNGC | 3 cut(s) | 450, 465, 657 |
| BlsI | GCNGC | 3 cut(s) | 451, 466, 658 |
| Bme1390I | CCNGG | 1 cut(s) | 144 |
| Bme18I | GGWCC | 1 cut(s) | 430 |
| BmgBI | CACGTC | 1 cut(s) | 50 |
| BmgT120I | GGNCC | 1 cut(s) | 430 |
| BmrFI | CCNGG | 1 cut(s) | 144 |
| BmsI | GCATC | 3 cut(s) | 355, 364, 505 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 194 |
| Bse1I | ACTGG | 1 cut(s) | 180 |
| BseBI | CCWGG | 1 cut(s) | 144 |
| BseGI | GGATG | 5 cut(s) | 87, 296, 370, 380, 625 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 194 |
| BseMII | CTCAG | 1 cut(s) | 555 |
| BseNI | ACTGG | 1 cut(s) | 180 |
| BseRI | GAGGAG | 1 cut(s) | 619 |
| BseXI | GCAGC | 3 cut(s) | 436, 451, 643 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 194 |
| BsmI | GAATGC | 1 cut(s) | 172 |
| BspCNI | CTCAG | 1 cut(s) | 556 |
| BspMI | ACCTGC | 1 cut(s) | 469 |
| BspQI | GCTCTTC | 1 cut(s) | 483 |
| BsrI | ACTGG | 1 cut(s) | 180 |
| BssSI | CACGAG | 1 cut(s) | 595 |
| Bst2BI | CACGAG | 1 cut(s) | 595 |
| Bst2UI | CCWGG | 1 cut(s) | 144 |
| Bst4CI | ACNGT | 1 cut(s) | 434 |
| Bst6I | CTCTTC | 2 cut(s) | 84, 483 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 1 cut(s) | 510 |
| BstDEI | CTNAG | 3 cut(s) | 528, 564, 643 |
| BstF5I | GGATG | 5 cut(s) | 87, 296, 370, 380, 625 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 341 |
| BstNI | CCWGG | 1 cut(s) | 144 |
| BstNSI | RCATGY | 3 cut(s) | 218, 271, 512 |
| BstSCI | CCNGG | 1 cut(s) | 142 |
| BstV1I | GCAGC | 3 cut(s) | 436, 451, 643 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 281 |
| BtrI | CACGTC | 1 cut(s) | 50 |
| BtsCI | GGATG | 5 cut(s) | 87, 296, 370, 380, 625 |
| BtsI | GCAGTG | 1 cut(s) | 552 |
| BtsIMutI | CAGTG | 1 cut(s) | 552 |
| BveI | ACCTGC | 1 cut(s) | 469 |
| Cac8I | GCNNGC | 1 cut(s) | 510 |
| Cfr13I | GGNCC | 1 cut(s) | 430 |
| CsiI | ACCWGGT | 1 cut(s) | 142 |
| Csp6I | GTAC | 2 cut(s) | 147, 458 |
| CviAII | CATG | 5 cut(s) | 116, 215, 268, 336, 509 |
| CviJI | RGCY | 3 cut(s) | 449, 493, 642 |
| CviKI_1 | RGCY | 3 cut(s) | 449, 493, 642 |
| CviQI | GTAC | 2 cut(s) | 147, 458 |
| DdeI | CTNAG | 3 cut(s) | 528, 564, 643 |
| Eam1104I | CTCTTC | 2 cut(s) | 84, 483 |
| EarI | CTCTTC | 2 cut(s) | 84, 483 |
| Eco47I | GGWCC | 1 cut(s) | 430 |
| Eco57I | CTGAAG | 1 cut(s) | 30 |
| EcoRII | CCWGG | 1 cut(s) | 142 |
| EcoT22I | ATGCAT | 2 cut(s) | 346, 520 |
| FaeI | CATG | 5 cut(s) | 119, 218, 271, 339, 512 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 35, 63, 95 |
| FatI | CATG | 5 cut(s) | 115, 214, 267, 335, 508 |
| FauNDI | CATATG | 1 cut(s) | 346 |
| FblI | GTMKAC | 1 cut(s) | 587 |
| Fnu4HI | GCNGC | 3 cut(s) | 450, 465, 657 |
| FokI | GGATG | 5 cut(s) | 74, 303, 377, 387, 632 |
| Fsp4HI | GCNGC | 3 cut(s) | 450, 465, 657 |
| FspBI | CTAG | 1 cut(s) | 92 |
| GluI | GCNGC | 3 cut(s) | 450, 465, 657 |
| Hin1II | CATG | 5 cut(s) | 119, 218, 271, 339, 512 |
| HincII | GTYRAC | 1 cut(s) | 322 |
| HindII | GTYRAC | 1 cut(s) | 322 |
| HinfI | GANTC | 5 cut(s) | 5, 221, 524, 566, 584 |
| Hpy166II | GTNNAC | 5 cut(s) | 212, 322, 430, 570, 588 |
| Hpy188I | TCNGA | 2 cut(s) | 10, 565 |
| Hpy188III | TCNNGA | 1 cut(s) | 195 |
| Hpy8I | GTNNAC | 5 cut(s) | 212, 322, 430, 570, 588 |
| HpyCH4III | ACNGT | 1 cut(s) | 434 |
| HpyCH4IV | ACGT | 2 cut(s) | 49, 397 |
| HpyCH4V | TGCA | 5 cut(s) | 107, 344, 467, 518, 659 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 341 |
| HpyF3I | CTNAG | 3 cut(s) | 528, 564, 643 |
| HpySE526I | ACGT | 2 cut(s) | 49, 397 |
| Hsp92II | CATG | 5 cut(s) | 119, 218, 271, 339, 512 |
| LguI | GCTCTTC | 1 cut(s) | 483 |
| LmnI | GCTCC | 1 cut(s) | 276 |
| LpnPI | CCDG | 6 cut(s) | 53, 129, 156, 161, 180, 474 |
| Lsp1109I | GCAGC | 3 cut(s) | 436, 451, 643 |
| LweI | GCATC | 3 cut(s) | 355, 364, 505 |
| MabI | ACCWGGT | 1 cut(s) | 142 |
| MaeI | CTAG | 1 cut(s) | 92 |
| MaeII | ACGT | 2 cut(s) | 49, 397 |
| MboII | GAAGA | 5 cut(s) | 23, 71, 83, 107, 500 |
| MluCI | AATT | 4 cut(s) | 153, 182, 190, 578 |
| MlyI | GAGTC | 3 cut(s) | 230, 575, 593 |
| MnlI | CCTC | 8 cut(s) | 297, 310, 325, 372, 469, 597, 600, 610 |
| Mph1103I | ATGCAT | 2 cut(s) | 346, 520 |
| MseI | TTAA | 3 cut(s) | 231, 240, 483 |
| MslI | CAYNNNNRTG | 2 cut(s) | 363, 513 |
| MspR9I | CCNGG | 1 cut(s) | 144 |
| Mva1269I | GAATGC | 1 cut(s) | 172 |
| MvaI | CCWGG | 1 cut(s) | 144 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 341 |
| NdeI | CATATG | 1 cut(s) | 346 |
| NlaIII | CATG | 5 cut(s) | 119, 218, 271, 339, 512 |
| NsiI | ATGCAT | 2 cut(s) | 346, 520 |
| NspI | RCATGY | 3 cut(s) | 218, 271, 512 |
| PaeI | GCATGC | 1 cut(s) | 512 |
| PciI | ACATGT | 1 cut(s) | 214 |
| PciSI | GCTCTTC | 1 cut(s) | 483 |
| PctI | GAATGC | 1 cut(s) | 172 |
| PfeI | GAWTC | 2 cut(s) | 5, 524 |
| PkrI | GCNGC | 3 cut(s) | 451, 466, 658 |
| PleI | GAGTC | 3 cut(s) | 229, 574, 592 |
| PpsI | GAGTC | 3 cut(s) | 229, 574, 592 |
| PscI | ACATGT | 1 cut(s) | 214 |
| PsiI | TTATAA | 1 cut(s) | 23 |
| Psp6I | CCWGG | 1 cut(s) | 142 |
| PspGI | CCWGG | 1 cut(s) | 142 |
| PspPI | GGNCC | 1 cut(s) | 430 |
| RsaI | GTAC | 2 cut(s) | 148, 459 |
| RsaNI | GTAC | 2 cut(s) | 147, 458 |
| RseI | CAYNNNNRTG | 2 cut(s) | 363, 513 |
| SapI | GCTCTTC | 1 cut(s) | 483 |
| SaqAI | TTAA | 3 cut(s) | 231, 240, 483 |
| SatI | GCNGC | 3 cut(s) | 450, 465, 657 |
| Sau96I | GGNCC | 1 cut(s) | 430 |
| SchI | GAGTC | 3 cut(s) | 230, 575, 593 |
| ScrFI | CCNGG | 1 cut(s) | 144 |
| SetI | ASST | 7 cut(s) | 52, 145, 321, 400, 463, 495, 534 |
| SexAI | ACCWGGT | 1 cut(s) | 142 |
| SfaNI | GCATC | 3 cut(s) | 355, 364, 505 |
| SinI | GGWCC | 1 cut(s) | 430 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 363, 513 |
| SphI | GCATGC | 1 cut(s) | 512 |
| Sse9I | AATT | 4 cut(s) | 153, 182, 190, 578 |
| SspMI | CTAG | 1 cut(s) | 92 |
| StyD4I | CCNGG | 1 cut(s) | 142 |
| TaaI | ACNGT | 1 cut(s) | 434 |
| TaiI | ACGT | 2 cut(s) | 52, 400 |
| TaqI | TCGA | 1 cut(s) | 582 |
| TasI | AATT | 4 cut(s) | 153, 182, 190, 578 |
| TfiI | GAWTC | 2 cut(s) | 5, 524 |
| Tru1I | TTAA | 3 cut(s) | 231, 240, 483 |
| Tru9I | TTAA | 3 cut(s) | 231, 240, 483 |
| TscAI | CASTG | 1 cut(s) | 559 |
| TseI | GCWGC | 3 cut(s) | 449, 464, 656 |
| TspDTI | ATGAA | 2 cut(s) | 417, 501 |
| TspRI | CASTG | 1 cut(s) | 559 |
| VpaK11BI | GGWCC | 1 cut(s) | 430 |
| XapI | RAATTY | 2 cut(s) | 182, 578 |
| XceI | RCATGY | 3 cut(s) | 218, 271, 512 |
| XmiI | GTMKAC | 1 cut(s) | 587 |
| XspI | CTAG | 1 cut(s) | 92 |
| Zsp2I | ATGCAT | 2 cut(s) | 346, 520 |