Rw0G022020
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01021
OG0000261
Physical Location & Seq
Reverse (-)
11241 .. 14854
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022020.1

Sequence Viewer

Length: 657 bp
ATGGATCGAAGGCAAATTATGTTGCTCTTATTGGTGGAGCTGTGGCACATAGAGACAATGTGTGCTTGTACTATGCTTGTGCTATTGATGTTTAATTTGAGACGAAGGCGTTCTGAAAGACATGCTCTAAATAATCGTTCATTCGTTAGACATGAGACTCGTTTGAGTTATCTAGATAGTATTATAGGCAATAGTGACACCGAATGTGTACATGAATTAAGAATTGACCATGCGACTGGAACTGGAGCTGAGGTCCCTGCTGATATGATGGAGGAGCAGAGTAACAATGAAGATGGCATTGAAGTTGAGAATGACACAAGCCCCATGTCAATGAGCACGAGACAATCTCAGGTTAGTCAGAAAAAGAGGAAAAGAAATGATGAAGATAAAATAATGCTTGCATTGGATAAATTGTTTGAAGAATCTGGAAAAAGAATGCAAGCAGTGACTGATGCTATAGTGAAAGGTAATGAAGATCAATCTGATATTGCTACGGAACTTAAGAAAATGGGACTTTCTGTTCTAGACCAAAGTGAGGCATTGAAAATCATTTTGGATAAGCCCCAAAATATCTCTGTGTTCATGTCCTTAGATGATGAAGTGCGAAAAGTGTATGTGGATAACTTGCTTGTGCGCACTGATAGAGGATCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

218

Amino Acids

25.02

Weight (kDa)

5.24

Isoelectric Point (pI)

71.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18411 FvH4_1g22560 FvH4_1g24290 FvH4_1g24290 FvH4_1g24290 FvH4_1g29434 FvH4_2g04031 FvH4_2g04811 FvH4_2g07610 FvH4_2g09412 FvH4_3g15552 FvH4_3g30720 FvH4_4g05810 FvH4_4g09541 FvH4_4g15171 FvH4_6g09145 FvH4_6g49541 FvH4_7g00320 FvH4_7g11552
malus_domestica MD00G1072400.v1.1 MD01G1188100.v1.1 MD07G1132000.v1.1 MD08G1190700.v1.1 MD10G1011200.v1.1 MD14G1164100.v1.1 MD15G1289200.v1.1 MD15G1289300.v1.1 MD17G1245100.v1.1
prunus_persica Prupe.6G228600_v2.0.a1
pyrus_communis pycom01g07070 pycom02g06890 pycom02g06900 pycom02g13930 pycom04g07470 pycom04g11770 pycom04g21930 pycom05g08520 pycom06g04490 pycom06g09560 pycom07g03750 pycom08g08760 pycom08g13660 pycom09g11520 pycom09g14250 pycom09g14770 pycom10g00720 pycom10g01290 pycom10g10480 pycom10g15700 pycom12420g00130 pycom12g05380 pycom12g08750 pycom13g19010 pycom13g29080 pycom14g06210 pycom14g13680 pycom14g19850 pycom15g22070 pycom15g23440 pycom15g24950 pycom15g25280 pycom16g11100 pycom16g17080 pycom16g26310 pycom17g15650 pycom17g15670 pycom17g20370
rosa_chinensis RchiOBHm_Chr1g0373591 RchiOBHm_Chr2g0105551 RchiOBHm_Chr2g0106711 RchiOBHm_Chr2g0106721 RchiOBHm_Chr7g0219611
rosa_laevigata RLG00000013773 RLG00000017436 RLG00000017533
rosa_multiflora Rmu_co8001842.1_g000001 Rmu_co8158450.1_g000001 Rmu_sc0000516.1_g000067 Rmu_sc0000566.1_g000009 Rmu_sc0000965.1_g000025 Rmu_sc0001718.1_g000001 Rmu_sc0001719.1_g000020 Rmu_sc0001822.1_g000025 Rmu_sc0002219.1_g000003 Rmu_sc0002460.1_g000059 Rmu_sc0002757.1_g000001 Rmu_sc0002860.1_g000001 Rmu_sc0004531.1_g000001 Rmu_sc0005558.1_g000003 Rmu_sc0011218.1_g000001 Rmu_sc0011998.1_g000003 Rmu_sc0015560.1_g000002 Rmu_sc0015560.1_g000003
rosa_roxburghii Rroxscaffold_1G00004200 Rroxscaffold_1G00026570 Rroxscaffold_1G00040370 Rroxscaffold_1G00044120 Rroxscaffold_1G00049590 Rroxscaffold_1G00059030 Rroxscaffold_2G00108440 Rroxscaffold_2G00108850 Rroxscaffold_2G00130680 Rroxscaffold_2G00132980 Rroxscaffold_2G00136860 Rroxscaffold_3G00218610 Rroxscaffold_4G00309940 Rroxscaffold_6G00405560
rosa_rugosa Rorug02G0138000 Rorug02G0138100 Rorug02G0138100 Rorug06G0154000
rosa_samantha Rh1AG121500 Rh1CG116300 Rh2AG189000 Rh2AG509600 Rh2BG200200 Rh2CG194100 Rh2DG195300 Rh5AG114400 Rh5DG488800
rosa_wichuraiana Rw0G022020 Rw1G023650 Rw1G026380 Rw1G034750 Rw1G040770 Rw2G014870 Rw2G016560 Rw4G031190 Rw5G026570 Rw6G003020 Rw6G012060 Rw7G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 635
AclWI GGATC 2 cut(s) 12, 655
AfaI GTAC 2 cut(s) 70, 210
AfiI CCNNNNNNNGG 1 cut(s) 535
AflII CTTAAG 1 cut(s) 500
AgsI TTSAA 3 cut(s) 302, 419, 544
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AloI GAACNNNNNNTCC 2 cut(s) 504, 536
AluBI AGCT 2 cut(s) 40, 248
AluI AGCT 2 cut(s) 40, 248
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 4 cut(s) 47, 94, 149, 334
AlwI GGATC 2 cut(s) 12, 655
AlwNI CAGNNNCTG 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 109
AspLEI GCGC 1 cut(s) 636
AspS9I GGNCC 1 cut(s) 253
AvaII GGWCC 1 cut(s) 253
BauI CACGAG 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 249
BccI CCATC 2 cut(s) 262, 287
BcoDI GTCTC 4 cut(s) 47, 94, 149, 334
BfaI CTAG 2 cut(s) 173, 524
BfmI CTRYAG 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 500
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 255
BmsI GCATC 1 cut(s) 442
BplI GAGNNNNNCTC 2 cut(s) 331, 363
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse1I ACTGG 2 cut(s) 241, 247
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 2 cut(s) 240, 362
BseNI ACTGG 2 cut(s) 241, 247
BseRI GAGGAG 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 338
BslFI GGGAC 2 cut(s) 239, 525
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 4 cut(s) 47, 94, 149, 334
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 2 cut(s) 239, 525
BsmI GAATGC 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 338
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 3 cut(s) 4, 475, 647
BspCNI CTCAG 2 cut(s) 241, 361
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 2 cut(s) 12, 655
BspTI CTTAAG 1 cut(s) 500
BsrGI TGTACA 1 cut(s) 208
BsrI ACTGG 2 cut(s) 241, 247
BssMI GATC 3 cut(s) 4, 475, 647
BssSI CACGAG 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 337
BstAFI CTTAAG 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 399, 441
BstDEI CTNAG 3 cut(s) 249, 348, 589
BstHHI GCGC 1 cut(s) 636
BstKTI GATC 3 cut(s) 7, 478, 650
BstMAI GTCTC 4 cut(s) 47, 94, 149, 334
BstMBI GATC 3 cut(s) 4, 475, 647
BstNSI RCATGY 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 236
BstYI RGATCY 1 cut(s) 647
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 2 cut(s) 450, 636
Cac8I GCNNGC 2 cut(s) 399, 441
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 1 cut(s) 636
Cfr13I GGNCC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 69, 209
CviAII CATG 6 cut(s) 122, 152, 212, 230, 325, 583
CviJI RGCY 4 cut(s) 40, 248, 321, 562
CviKI_1 RGCY 4 cut(s) 40, 248, 321, 562
CviQI GTAC 2 cut(s) 69, 209
DdeI CTNAG 3 cut(s) 249, 348, 589
DpnI GATC 3 cut(s) 6, 477, 649
DpnII GATC 3 cut(s) 4, 475, 647
Eco47I GGWCC 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 6 cut(s) 125, 155, 215, 233, 328, 586
FaqI GGGAC 2 cut(s) 239, 525
FatI CATG 6 cut(s) 121, 151, 211, 229, 324, 582
FspAI RTGCGCAY 1 cut(s) 635
FspBI CTAG 2 cut(s) 173, 524
FspI TGCGCA 1 cut(s) 635
GlaI GCGC 1 cut(s) 635
GsuI CTGGAG 1 cut(s) 264
HhaI GCGC 1 cut(s) 636
Hin1II CATG 6 cut(s) 125, 155, 215, 233, 328, 586
Hin6I GCGC 1 cut(s) 634
HinP1I GCGC 1 cut(s) 634
HinfI GANTC 2 cut(s) 157, 422
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 3 cut(s) 115, 360, 484
Hpy188III TCNNGA 3 cut(s) 173, 426, 524
Hpy8I GTNNAC 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 3, 99
HpyCH4V TGCA 2 cut(s) 401, 439
HpyF3I CTNAG 3 cut(s) 249, 348, 589
Hsp92II CATG 6 cut(s) 125, 155, 215, 233, 328, 586
HspAI GCGC 1 cut(s) 634
Kzo9I GATC 3 cut(s) 4, 475, 647
LmnI GCTCC 3 cut(s) 37, 245, 274
LpnPI CCDG 5 cut(s) 222, 228, 270, 335, 411
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 173, 524
MaeIII GTNAC 3 cut(s) 194, 281, 445
MalI GATC 3 cut(s) 6, 477, 649
MboI GATC 3 cut(s) 4, 475, 647
MboII GAAGA 4 cut(s) 302, 395, 431, 485
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 1 cut(s) 338
MluCI AATT 5 cut(s) 15, 94, 215, 222, 410
MlyI GAGTC 1 cut(s) 151
MnlI CCTC 5 cut(s) 244, 265, 360, 529, 638
MroXI GAANNNNTTC 1 cut(s) 109
MseI TTAA 3 cut(s) 93, 218, 501
MslI CAYNNNNRTG 1 cut(s) 329
MspCI CTTAAG 1 cut(s) 500
Mva1269I GAATGC 1 cut(s) 441
NdeII GATC 3 cut(s) 4, 475, 647
NlaIII CATG 6 cut(s) 125, 155, 215, 233, 328, 586
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 2 cut(s) 194, 445
NsbI TGCGCA 1 cut(s) 635
NspI RCATGY 1 cut(s) 125
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 422
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PpuMI RGGWCCY 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 253
PspPPI RGGWCCY 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 647
RsaI GTAC 2 cut(s) 70, 210
RsaNI GTAC 2 cut(s) 69, 209
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 3 cut(s) 93, 218, 501
Sau3AI GATC 3 cut(s) 4, 475, 647
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 1 cut(s) 151
SduI GDGCHC 1 cut(s) 338
SetI ASST 5 cut(s) 42, 250, 255, 354, 469
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 5 cut(s) 15, 94, 215, 222, 410
SspMI CTAG 2 cut(s) 173, 524
TaqI TCGA 1 cut(s) 7
TasI AATT 5 cut(s) 15, 94, 215, 222, 410
TatI WGTACW 2 cut(s) 68, 208
TfiI GAWTC 1 cut(s) 422
Tru1I TTAA 3 cut(s) 93, 218, 501
Tru9I TTAA 3 cut(s) 93, 218, 501
TscAI CASTG 2 cut(s) 450, 643
TseFI GTSAC 2 cut(s) 194, 445
Tsp45I GTSAC 2 cut(s) 194, 445
TspDTI ATGAA 7 cut(s) 129, 228, 303, 396, 486, 571, 612
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 2 cut(s) 450, 643
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 253
XbaI TCTAGA 2 cut(s) 172, 523
XceI RCATGY 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 109
XspI CTAG 2 cut(s) 173, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.