Rw1G023650
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000261
Physical Location & Seq
Forward (+)
49594776 .. 49598153
3378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023650.1

Sequence Viewer

Length: 681 bp
ATGGATCGAAGGCAAATTATGTTGCTCTTATTGGTGGAGTTGTGGCACCTAGAGACAATGTGTGCTTGTACTATGCTTGTGCTATTGATGTTTAATTTGAGACGAAGACGTGCTGAAAGATATGCTCTAAATAATCGTTCATTCGTTAGACGTGAGACTCGTTTGAGTTATCTAGATAGTATTATAGGCAATAGTGACACCGAATGTGTACATGAATTAAGAATGGATAGAAGGACTTTTGGCCTTTTTGACCGTGCGACTGGAACTGGAGCTGAGGTCCCTGCTGATATGATGGAGGAGCAGAGCAACAATGAAGATGGCATTGAAGTTGAGAATGACACAAGCCCCATGTCAATGAGCACGGGACAATCTCAGGTTAGTCAGAAAAAGAGGAAAAGAAATGATGAAGATAAAATAATGCTTGCATTGGATAAATTGTTTGAAGAATCTGGAAAAAGAATGCAAGCAGTGACTGATGCTATAGTGAAAGGTAATGAAGATCGATCTGATATTGCTAAGGAACTTAAGAAAATGGGACTTTCTGTTCTAGACCAAATTGAGGCATTGAAAATCATTTTGGATAAGCCCCAAAATATCTCTGTGTTCATGTCCTTAGATGATGAAGTGCGAAAAGTGTATGTGGATAACTTGCTTGCGGGCACTGCTAGAGGATCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

226

Amino Acids

25.89

Weight (kDa)

5.45

Isoelectric Point (pI)

64.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18411 FvH4_1g22560 FvH4_1g24290 FvH4_1g24290 FvH4_1g24290 FvH4_1g29434 FvH4_2g04031 FvH4_2g04811 FvH4_2g07610 FvH4_2g09412 FvH4_3g15552 FvH4_3g30720 FvH4_4g05810 FvH4_4g09541 FvH4_4g15171 FvH4_6g09145 FvH4_6g49541 FvH4_7g00320 FvH4_7g11552
malus_domestica MD00G1072400.v1.1 MD01G1188100.v1.1 MD07G1132000.v1.1 MD08G1190700.v1.1 MD10G1011200.v1.1 MD14G1164100.v1.1 MD15G1289200.v1.1 MD15G1289300.v1.1 MD17G1245100.v1.1
prunus_persica Prupe.6G228600_v2.0.a1
pyrus_communis pycom01g07070 pycom02g06890 pycom02g06900 pycom02g13930 pycom04g07470 pycom04g11770 pycom04g21930 pycom05g08520 pycom06g04490 pycom06g09560 pycom07g03750 pycom08g08760 pycom08g13660 pycom09g11520 pycom09g14250 pycom09g14770 pycom10g00720 pycom10g01290 pycom10g10480 pycom10g15700 pycom12420g00130 pycom12g05380 pycom12g08750 pycom13g19010 pycom13g29080 pycom14g06210 pycom14g13680 pycom14g19850 pycom15g22070 pycom15g23440 pycom15g24950 pycom15g25280 pycom16g11100 pycom16g17080 pycom16g26310 pycom17g15650 pycom17g15670 pycom17g20370
rosa_chinensis RchiOBHm_Chr1g0373591 RchiOBHm_Chr2g0105551 RchiOBHm_Chr2g0106711 RchiOBHm_Chr2g0106721 RchiOBHm_Chr7g0219611
rosa_laevigata RLG00000013773 RLG00000017436 RLG00000017533
rosa_multiflora Rmu_co8001842.1_g000001 Rmu_co8158450.1_g000001 Rmu_sc0000516.1_g000067 Rmu_sc0000566.1_g000009 Rmu_sc0000965.1_g000025 Rmu_sc0001718.1_g000001 Rmu_sc0001719.1_g000020 Rmu_sc0001822.1_g000025 Rmu_sc0002219.1_g000003 Rmu_sc0002460.1_g000059 Rmu_sc0002757.1_g000001 Rmu_sc0002860.1_g000001 Rmu_sc0004531.1_g000001 Rmu_sc0005558.1_g000003 Rmu_sc0011218.1_g000001 Rmu_sc0011998.1_g000003 Rmu_sc0015560.1_g000002 Rmu_sc0015560.1_g000003
rosa_roxburghii Rroxscaffold_1G00004200 Rroxscaffold_1G00026570 Rroxscaffold_1G00040370 Rroxscaffold_1G00044120 Rroxscaffold_1G00049590 Rroxscaffold_1G00059030 Rroxscaffold_2G00108440 Rroxscaffold_2G00108850 Rroxscaffold_2G00130680 Rroxscaffold_2G00132980 Rroxscaffold_2G00136860 Rroxscaffold_3G00218610 Rroxscaffold_4G00309940 Rroxscaffold_6G00405560
rosa_rugosa Rorug02G0138000 Rorug02G0138100 Rorug02G0138100 Rorug06G0154000
rosa_samantha Rh1AG121500 Rh1CG116300 Rh2AG189000 Rh2AG509600 Rh2BG200200 Rh2CG194100 Rh2DG195300 Rh5AG114400 Rh5DG488800
rosa_wichuraiana Rw0G022020 Rw1G023650 Rw1G026380 Rw1G034750 Rw1G040770 Rw2G014870 Rw2G016560 Rw4G031190 Rw5G026570 Rw6G003020 Rw6G012060 Rw7G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 45
AciI CCGC 1 cut(s) 656
AclWI GGATC 2 cut(s) 12, 679
AfaI GTAC 2 cut(s) 70, 210
AfiI CCNNNNNNNGG 1 cut(s) 559
AflII CTTAAG 1 cut(s) 524
AgsI TTSAA 3 cut(s) 326, 443, 568
AjiI CACGTC 2 cut(s) 110, 152
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AloI GAACNNNNNNTCC 2 cut(s) 528, 560
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
Alw21I GWGCWC 1 cut(s) 362
Alw26I GTCTC 3 cut(s) 47, 94, 149
AlwI GGATC 2 cut(s) 12, 679
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 277
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 662
BanI GGYRCC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 362
BbvCI CCTCAGC 1 cut(s) 273
BccI CCATC 2 cut(s) 286, 311
BcoDI GTCTC 3 cut(s) 47, 94, 149
BfaI CTAG 4 cut(s) 50, 173, 548, 666
BfmI CTRYAG 1 cut(s) 480
BfrI CTTAAG 1 cut(s) 524
Bme18I GGWCC 1 cut(s) 277
BmgBI CACGTC 2 cut(s) 110, 152
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 47, 279
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 2 cut(s) 273, 516
Bsa29I ATCGAT 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 2 cut(s) 265, 271
BseCI ATCGAT 1 cut(s) 502
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 2 cut(s) 264, 386
BseNI ACTGG 2 cut(s) 265, 271
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 662
BshFI GGCC 1 cut(s) 243
BshNI GGYRCC 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 502
BsiHKAI GWGCWC 1 cut(s) 362
BslFI GGGAC 3 cut(s) 263, 378, 549
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 3 cut(s) 47, 94, 149
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 3 cut(s) 263, 378, 549
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 362, 662
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 4 cut(s) 4, 499, 503, 671
BspACI CCGC 1 cut(s) 656
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 265, 385
BspDI ATCGAT 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 47, 279
BspPI GGATC 2 cut(s) 12, 679
BspT107I GGYRCC 1 cut(s) 45
BspTI CTTAAG 1 cut(s) 524
BsrGI TGTACA 1 cut(s) 208
BsrI ACTGG 2 cut(s) 265, 271
BssMI GATC 4 cut(s) 4, 499, 503, 671
Bst4CI ACNGT 1 cut(s) 254
BstAFI CTTAAG 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 208
BstC8I GCNNGC 4 cut(s) 423, 465, 654, 658
BstDEI CTNAG 4 cut(s) 273, 372, 516, 613
BstKTI GATC 4 cut(s) 7, 502, 506, 674
BstMAI GTCTC 3 cut(s) 47, 94, 149
BstMBI GATC 4 cut(s) 4, 499, 503, 671
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 662
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 671
BstYI RGATCY 1 cut(s) 671
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 502
BtrI CACGTC 2 cut(s) 110, 152
BtsI GCAGTG 2 cut(s) 474, 660
BtsIMutI CAGTG 2 cut(s) 474, 660
Cac8I GCNNGC 4 cut(s) 423, 465, 654, 658
CaiI CAGNNNCTG 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 502
Csp6I GTAC 2 cut(s) 69, 209
CviAII CATG 3 cut(s) 212, 349, 607
CviJI RGCY 4 cut(s) 243, 272, 345, 586
CviKI_1 RGCY 4 cut(s) 243, 272, 345, 586
CviQI GTAC 2 cut(s) 69, 209
DdeI CTNAG 4 cut(s) 273, 372, 516, 613
DpnI GATC 4 cut(s) 6, 501, 505, 673
DpnII GATC 4 cut(s) 4, 499, 503, 671
Eco47I GGWCC 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 277
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 3 cut(s) 215, 352, 610
FaqI GGGAC 3 cut(s) 263, 378, 549
FatI CATG 3 cut(s) 211, 348, 606
FauI CCCGC 1 cut(s) 649
FspBI CTAG 4 cut(s) 50, 173, 548, 666
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 1 cut(s) 243
Hin1II CATG 3 cut(s) 215, 352, 610
HinfI GANTC 2 cut(s) 157, 446
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 2 cut(s) 384, 508
Hpy188III TCNNGA 3 cut(s) 173, 450, 548
Hpy8I GTNNAC 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 3, 225
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4IV ACGT 2 cut(s) 109, 151
HpyCH4V TGCA 2 cut(s) 425, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 4 cut(s) 273, 372, 516, 613
HpySE526I ACGT 2 cut(s) 109, 151
Hsp92II CATG 3 cut(s) 215, 352, 610
Kzo9I GATC 4 cut(s) 4, 499, 503, 671
LmnI GCTCC 2 cut(s) 269, 298
LpnPI CCDG 5 cut(s) 246, 252, 294, 359, 435
LweI GCATC 1 cut(s) 466
MaeI CTAG 4 cut(s) 50, 173, 548, 666
MaeII ACGT 2 cut(s) 109, 151
MaeIII GTNAC 2 cut(s) 194, 469
MalI GATC 4 cut(s) 6, 501, 505, 673
MboI GATC 4 cut(s) 4, 499, 503, 671
MboII GAAGA 5 cut(s) 117, 326, 419, 455, 509
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 2 cut(s) 362, 662
MluCI AATT 5 cut(s) 15, 94, 215, 434, 555
MlyI GAGTC 1 cut(s) 151
MnlI CCTC 5 cut(s) 268, 289, 384, 553, 662
MseI TTAA 3 cut(s) 93, 218, 525
MslI CAYNNNNRTG 1 cut(s) 353
MspCI CTTAAG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 662
NdeII GATC 4 cut(s) 4, 499, 503, 671
NlaIII CATG 3 cut(s) 215, 352, 610
NlaIV GGNNCC 2 cut(s) 47, 279
NmuCI GTSAC 2 cut(s) 194, 469
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 446
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PpuMI RGGWCCY 1 cut(s) 277
Psp5II RGGWCCY 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 47, 279
PspPI GGNCC 1 cut(s) 277
PspPPI RGGWCCY 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 671
RsaI GTAC 2 cut(s) 70, 210
RsaNI GTAC 2 cut(s) 69, 209
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 3 cut(s) 93, 218, 525
Sau3AI GATC 4 cut(s) 4, 499, 503, 671
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 1 cut(s) 151
SduI GDGCHC 2 cut(s) 362, 662
SetI ASST 7 cut(s) 51, 112, 154, 274, 279, 378, 493
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 1 cut(s) 480
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 5 cut(s) 15, 94, 215, 434, 555
SsiI CCGC 1 cut(s) 656
SspMI CTAG 4 cut(s) 50, 173, 548, 666
TaaI ACNGT 1 cut(s) 254
TaiI ACGT 2 cut(s) 112, 154
TaqI TCGA 2 cut(s) 7, 502
TasI AATT 5 cut(s) 15, 94, 215, 434, 555
TatI WGTACW 2 cut(s) 68, 208
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 3 cut(s) 93, 218, 525
Tru9I TTAA 3 cut(s) 93, 218, 525
TscAI CASTG 2 cut(s) 474, 667
TseFI GTSAC 2 cut(s) 194, 469
Tsp45I GTSAC 2 cut(s) 194, 469
TspDTI ATGAA 7 cut(s) 129, 228, 327, 420, 510, 595, 636
TspRI CASTG 2 cut(s) 474, 667
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 277
XbaI TCTAGA 2 cut(s) 172, 547
XspI CTAG 4 cut(s) 50, 173, 548, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.