pycom06g09560

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000261
Physical Location & Seq
Forward (+)
14865367 .. 14866157
791 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g09560.1

Sequence Viewer

Length: 624 bp
ATGAAATGTAATATTACGGTTTCTTTGATAGATTATATGGCCACCTCACGTAATTGGAATGATCATGACGAGGATGTACTGCTTACCATCCTCGAGGAGATGGTAGTTGATGGTGTTAGGTGTGAGACCGGCAGTTTTAAGGATGGTACATTTGTAATGGTTGCCACCAAGATGAGGGAGATGATTCCGGGCATTAATATAGAGCCAAAGCATATACAAAACAAGCTGAAGCGTCTAAAAGAAAAGTATTCCTCTGCATATGACATGATGAATACCTCTGGATTTGGTTGGGATGATGAAAAAAAATGTGTTGTTGTGGATAGTGACGATGTACTTCAGCTGTGTGTGAAGAAACATCCTAATGCATCTTACAAACCAAATAAGCCATTTTCGTTGTATCCACGCTTATGTACGGTGTTTGGGAGAGATCGAGCCAAGGGTAGTATGGCTGAATCAGCAGCAGATGCAATTGAAAACATGGGATTGGAAAATCAGGATGATGATGAAACATTGAGATGCCTCCGACTTCACCTACCCCATCTCCTTCTATTGGTACATCCAGTGCATCTCAACCTATTAGGAAGAGGAAAAGGAATAAGAATAATGCTGATGCAAATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

208

Amino Acids

23.59

Weight (kDa)

6.38

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 17 - 97 3.8e-08 Myb/SANT-like DNA-binding domain
Myb_DNA-bind_3 PF12776 18 - 108 8.1e-12 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18411 FvH4_1g22560 FvH4_1g24290 FvH4_1g24290 FvH4_1g24290 FvH4_1g29434 FvH4_2g04031 FvH4_2g04811 FvH4_2g07610 FvH4_2g09412 FvH4_3g15552 FvH4_3g30720 FvH4_4g05810 FvH4_4g09541 FvH4_4g15171 FvH4_6g09145 FvH4_6g49541 FvH4_7g00320 FvH4_7g11552
malus_domestica MD00G1072400.v1.1 MD01G1188100.v1.1 MD07G1132000.v1.1 MD08G1190700.v1.1 MD10G1011200.v1.1 MD14G1164100.v1.1 MD15G1289200.v1.1 MD15G1289300.v1.1 MD17G1245100.v1.1
prunus_persica Prupe.6G228600_v2.0.a1
pyrus_communis pycom01g07070 pycom02g06890 pycom02g06900 pycom02g13930 pycom04g07470 pycom04g11770 pycom04g21930 pycom05g08520 pycom06g04490 pycom06g09560 pycom07g03750 pycom08g08760 pycom08g13660 pycom09g11520 pycom09g14250 pycom09g14770 pycom10g00720 pycom10g01290 pycom10g10480 pycom10g15700 pycom12420g00130 pycom12g05380 pycom12g08750 pycom13g19010 pycom13g29080 pycom14g06210 pycom14g13680 pycom14g19850 pycom15g22070 pycom15g23440 pycom15g24950 pycom15g25280 pycom16g11100 pycom16g17080 pycom16g26310 pycom17g15650 pycom17g15670 pycom17g20370
rosa_chinensis RchiOBHm_Chr1g0373591 RchiOBHm_Chr2g0105551 RchiOBHm_Chr2g0106711 RchiOBHm_Chr2g0106721 RchiOBHm_Chr7g0219611
rosa_laevigata RLG00000013773 RLG00000017436 RLG00000017533
rosa_multiflora Rmu_co8001842.1_g000001 Rmu_co8158450.1_g000001 Rmu_sc0000516.1_g000067 Rmu_sc0000566.1_g000009 Rmu_sc0000965.1_g000025 Rmu_sc0001718.1_g000001 Rmu_sc0001719.1_g000020 Rmu_sc0001822.1_g000025 Rmu_sc0002219.1_g000003 Rmu_sc0002460.1_g000059 Rmu_sc0002757.1_g000001 Rmu_sc0002860.1_g000001 Rmu_sc0004531.1_g000001 Rmu_sc0005558.1_g000003 Rmu_sc0011218.1_g000001 Rmu_sc0011998.1_g000003 Rmu_sc0015560.1_g000002 Rmu_sc0015560.1_g000003
rosa_roxburghii Rroxscaffold_1G00004200 Rroxscaffold_1G00026570 Rroxscaffold_1G00040370 Rroxscaffold_1G00044120 Rroxscaffold_1G00049590 Rroxscaffold_1G00059030 Rroxscaffold_2G00108440 Rroxscaffold_2G00108850 Rroxscaffold_2G00130680 Rroxscaffold_2G00132980 Rroxscaffold_2G00136860 Rroxscaffold_3G00218610 Rroxscaffold_4G00309940 Rroxscaffold_6G00405560
rosa_rugosa Rorug02G0138000 Rorug02G0138100 Rorug02G0138100 Rorug06G0154000
rosa_samantha Rh1AG121500 Rh1CG116300 Rh2AG189000 Rh2AG509600 Rh2BG200200 Rh2CG194100 Rh2DG195300 Rh5AG114400 Rh5DG488800
rosa_wichuraiana Rw0G022020 Rw1G023650 Rw1G026380 Rw1G034750 Rw1G040770 Rw2G014870 Rw2G016560 Rw4G031190 Rw5G026570 Rw6G003020 Rw6G012060 Rw7G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 2 cut(s) 248, 320
AfaI GTAC 5 cut(s) 78, 148, 333, 412, 555
AfiI CCNNNNNNNGG 2 cut(s) 174, 550
AgsI TTSAA 1 cut(s) 473
AluBI AGCT 2 cut(s) 226, 340
AluI AGCT 2 cut(s) 226, 340
Alw26I GTCTC 1 cut(s) 119
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 1 cut(s) 458
AseI ATTAAT 1 cut(s) 195
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BalI TGGCCA 1 cut(s) 41
BbvI GCAGC 1 cut(s) 470
BccI CCATC 5 cut(s) 94, 95, 104, 137, 546
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 119
BfuI GTATCC 1 cut(s) 408
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme1390I CCNGG 1 cut(s) 189
BmeT110I CYCGRG 1 cut(s) 92
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 5 cut(s) 374, 454, 506, 574, 600
BpuMI CCSGG 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 50
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 550
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 560
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 7 cut(s) 79, 87, 148, 298, 355, 502, 556
BseLI CCNNNNNNNGG 2 cut(s) 174, 550
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 470
BshFI GGCC 1 cut(s) 41
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 2 cut(s) 129, 188
BslI CCNNNNNNNGG 2 cut(s) 174, 550
BsmAI GTCTC 1 cut(s) 119
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 92
Bsp143I GATC 2 cut(s) 61, 427
BspANI GGCC 1 cut(s) 41
BspHI TCATGA 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 2 cut(s) 61, 427
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 19, 415
Bst6I CTCTTC 1 cut(s) 577
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 50
BstF5I GGATG 7 cut(s) 79, 87, 148, 298, 355, 502, 556
BstKTI GATC 2 cut(s) 64, 430
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 2 cut(s) 61, 427
BstMWI GCNNNNNNNGC 2 cut(s) 455, 464
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 470
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 41
BtsCI GGATG 7 cut(s) 79, 87, 148, 298, 355, 502, 556
BtsIMutI CAGTG 1 cut(s) 567
CciI TCATGA 1 cut(s) 64
Cfr10I RCCGGY 1 cut(s) 128
CseI GACGC 1 cut(s) 221
Csp6I GTAC 5 cut(s) 77, 147, 332, 411, 554
CviAII CATG 3 cut(s) 65, 265, 478
CviJI RGCY 7 cut(s) 41, 205, 226, 340, 385, 434, 449
CviKI_1 RGCY 7 cut(s) 41, 205, 226, 340, 385, 434, 449
CviQI GTAC 5 cut(s) 77, 147, 332, 411, 554
DpnI GATC 2 cut(s) 63, 429
DpnII GATC 2 cut(s) 61, 427
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 577
EarI CTCTTC 1 cut(s) 577
Eco130I CCWWGG 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 119
Eco57I CTGAAG 2 cut(s) 248, 320
Eco88I CYCGRG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 435
EcoT22I ATGCAT 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 3 cut(s) 68, 268, 481
FatI CATG 3 cut(s) 64, 264, 477
FauNDI CATATG 1 cut(s) 259
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 7 cut(s) 74, 86, 155, 305, 342, 509, 543
Fsp4HI GCNGC 1 cut(s) 459
GluI GCNGC 1 cut(s) 459
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 129, 188
HgaI GACGC 1 cut(s) 221
Hin1II CATG 3 cut(s) 68, 268, 481
HinfI GANTC 2 cut(s) 184, 452
HpaII CCGG 2 cut(s) 129, 188
HphI GGTGA 1 cut(s) 521
Hpy188I TCNGA 1 cut(s) 524
Hpy188III TCNNGA 3 cut(s) 65, 279, 494
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 2 cut(s) 19, 415
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 5 cut(s) 257, 365, 467, 565, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 464
HpySE526I ACGT 1 cut(s) 49
Hsp92II CATG 3 cut(s) 68, 268, 481
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 61, 427
LpnPI CCDG 5 cut(s) 142, 201, 264, 479, 573
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 5 cut(s) 374, 454, 506, 574, 600
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 323
MalI GATC 2 cut(s) 63, 429
MboI GATC 2 cut(s) 61, 427
MboII GAAGA 2 cut(s) 361, 594
MfeI CAATTG 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 2 cut(s) 52, 468
MluNI TGGCCA 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 9 cut(s) 55, 64, 88, 101, 168, 262, 286, 530, 578
Mox20I TGGCCA 1 cut(s) 41
Mph1103I ATGCAT 1 cut(s) 367
MscI TGGCCA 1 cut(s) 41
MseI TTAA 2 cut(s) 138, 195
MslI CAYNNNNRTG 4 cut(s) 170, 360, 406, 514
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 2 cut(s) 129, 188
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 455, 464
NciI CCSGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 259
NdeII GATC 2 cut(s) 61, 427
NlaIII CATG 3 cut(s) 68, 268, 481
NmuCI GTSAC 1 cut(s) 323
NsiI ATGCAT 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 92
PagI TCATGA 1 cut(s) 64
PfeI GAWTC 2 cut(s) 184, 452
PkrI GCNGC 1 cut(s) 460
Ppu21I YACGTR 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 195
PspXI VCTCGAGB 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 340
RsaI GTAC 5 cut(s) 78, 148, 333, 412, 555
RsaNI GTAC 5 cut(s) 77, 147, 332, 411, 554
RseI CAYNNNNRTG 4 cut(s) 170, 360, 406, 514
SaqAI TTAA 2 cut(s) 138, 195
SatI GCNGC 1 cut(s) 459
Sau3AI GATC 2 cut(s) 61, 427
ScrFI CCNGG 1 cut(s) 189
SetI ASST 8 cut(s) 47, 52, 122, 228, 278, 342, 534, 576
SfaNI GCATC 5 cut(s) 374, 454, 506, 574, 600
Sfr274I CTCGAG 1 cut(s) 92
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 4 cut(s) 170, 360, 406, 514
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 2 cut(s) 52, 468
SspI AATATT 2 cut(s) 13, 618
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 19, 415
TaiI ACGT 1 cut(s) 52
TaqI TCGA 2 cut(s) 93, 430
TasI AATT 2 cut(s) 52, 468
TatI WGTACW 2 cut(s) 76, 331
TfiI GAWTC 2 cut(s) 184, 452
Tru1I TTAA 2 cut(s) 138, 195
Tru9I TTAA 2 cut(s) 138, 195
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 4 cut(s) 17, 284, 312, 519
TspRI CASTG 1 cut(s) 567
VspI ATTAAT 1 cut(s) 195
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XhoI CTCGAG 1 cut(s) 92
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.