DNA ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 129 |
| AgsI | TTSAA | 2 cut(s) | 22, 337 |
| AluBI | AGCT | 1 cut(s) | 119 |
| AluI | AGCT | 1 cut(s) | 119 |
| Ama87I | CYCGRG | 1 cut(s) | 267 |
| ApeKI | GCWGC | 1 cut(s) | 57 |
| AspS9I | GGNCC | 1 cut(s) | 163 |
| AvaI | CYCGRG | 1 cut(s) | 267 |
| AvaII | GGWCC | 1 cut(s) | 163 |
| BbvCI | CCTCAGC | 1 cut(s) | 159 |
| BbvI | GCAGC | 1 cut(s) | 69 |
| BccI | CCATC | 2 cut(s) | 75, 131 |
| BciVI | GTATCC | 2 cut(s) | 39, 108 |
| BfaI | CTAG | 1 cut(s) | 116 |
| BfuI | GTATCC | 2 cut(s) | 39, 108 |
| BisI | GCNGC | 1 cut(s) | 58 |
| BlsI | GCNGC | 1 cut(s) | 59 |
| Bme18I | GGWCC | 1 cut(s) | 163 |
| BmeT110I | CYCGRG | 1 cut(s) | 267 |
| BmgT120I | GGNCC | 1 cut(s) | 163 |
| BmiI | GGNNCC | 1 cut(s) | 165 |
| Bpu10I | CCTNAGC | 1 cut(s) | 159 |
| BsaAI | YACGTR | 1 cut(s) | 50 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 124, 154 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 129 |
| Bse1I | ACTGG | 1 cut(s) | 151 |
| BseGI | GGATG | 1 cut(s) | 86 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 129 |
| BseMII | CTCAG | 1 cut(s) | 150 |
| BseNI | ACTGG | 1 cut(s) | 151 |
| BseRI | GAGGAG | 1 cut(s) | 197 |
| BseXI | GCAGC | 1 cut(s) | 69 |
| Bsh1285I | CGRYCG | 1 cut(s) | 277 |
| BsiEI | CGRYCG | 1 cut(s) | 277 |
| BsiHKCI | CYCGRG | 1 cut(s) | 267 |
| BslFI | GGGAC | 2 cut(s) | 149, 273 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 129 |
| BsmFI | GGGAC | 2 cut(s) | 149, 273 |
| BsmI | GAATGC | 1 cut(s) | 359 |
| BsoBI | CYCGRG | 1 cut(s) | 267 |
| BspCNI | CTCAG | 1 cut(s) | 151 |
| BspLI | GGNNCC | 1 cut(s) | 165 |
| BsrI | ACTGG | 1 cut(s) | 151 |
| Bst4CI | ACNGT | 1 cut(s) | 140 |
| BstBAI | YACGTR | 1 cut(s) | 50 |
| BstC8I | GCNNGC | 2 cut(s) | 317, 359 |
| BstDEI | CTNAG | 1 cut(s) | 159 |
| BstF5I | GGATG | 1 cut(s) | 86 |
| BstMCI | CGRYCG | 1 cut(s) | 277 |
| BstSNI | TACGTA | 1 cut(s) | 50 |
| BstV1I | GCAGC | 1 cut(s) | 69 |
| BsuI | GTATCC | 2 cut(s) | 39, 108 |
| BtsCI | GGATG | 1 cut(s) | 86 |
| Cac8I | GCNNGC | 2 cut(s) | 317, 359 |
| Cfr13I | GGNCC | 1 cut(s) | 163 |
| CviAII | CATG | 1 cut(s) | 235 |
| CviJI | RGCY | 3 cut(s) | 119, 158, 231 |
| CviKI_1 | RGCY | 3 cut(s) | 119, 158, 231 |
| DdeI | CTNAG | 1 cut(s) | 159 |
| Eco105I | TACGTA | 1 cut(s) | 50 |
| Eco32I | GATATC | 1 cut(s) | 6 |
| Eco47I | GGWCC | 1 cut(s) | 163 |
| Eco88I | CYCGRG | 1 cut(s) | 267 |
| EcoO109I | RGGNCCY | 1 cut(s) | 163 |
| EcoRV | GATATC | 1 cut(s) | 6 |
| FaeI | CATG | 1 cut(s) | 238 |
| FaiI | YATR | 5 cut(s) | 42, 176, 209, 236, 313 |
| FaqI | GGGAC | 2 cut(s) | 149, 273 |
| FatI | CATG | 1 cut(s) | 234 |
| Fnu4HI | GCNGC | 1 cut(s) | 58 |
| FokI | GGATG | 1 cut(s) | 93 |
| Fsp4HI | GCNGC | 1 cut(s) | 58 |
| FspBI | CTAG | 1 cut(s) | 116 |
| GluI | GCNGC | 1 cut(s) | 58 |
| Hin1II | CATG | 1 cut(s) | 238 |
| HinfI | GANTC | 1 cut(s) | 340 |
| Hpy188III | TCNNGA | 1 cut(s) | 344 |
| HpyCH4III | ACNGT | 1 cut(s) | 140 |
| HpyCH4IV | ACGT | 1 cut(s) | 49 |
| HpyCH4V | TGCA | 4 cut(s) | 17, 57, 319, 357 |
| HpyF3I | CTNAG | 1 cut(s) | 159 |
| HpySE526I | ACGT | 1 cut(s) | 49 |
| Hsp92II | CATG | 1 cut(s) | 238 |
| LmnI | GCTCC | 1 cut(s) | 184 |
| LpnPI | CCDG | 3 cut(s) | 132, 180, 329 |
| Lsp1109I | GCAGC | 1 cut(s) | 69 |
| MaeI | CTAG | 1 cut(s) | 116 |
| MaeII | ACGT | 1 cut(s) | 49 |
| MaeIII | GTNAC | 1 cut(s) | 134 |
| MboII | GAAGA | 3 cut(s) | 212, 313, 349 |
| MluCI | AATT | 2 cut(s) | 308, 328 |
| MmeI | TCCRAC | 1 cut(s) | 159 |
| MnlI | CCTC | 4 cut(s) | 154, 175, 237, 278 |
| MslI | CAYNNNNRTG | 1 cut(s) | 239 |
| Mva1269I | GAATGC | 1 cut(s) | 359 |
| NlaIII | CATG | 1 cut(s) | 238 |
| NlaIV | GGNNCC | 1 cut(s) | 165 |
| NmuCI | GTSAC | 1 cut(s) | 134 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 274 |
| PctI | GAATGC | 1 cut(s) | 359 |
| PfeI | GAWTC | 1 cut(s) | 340 |
| PkrI | GCNGC | 1 cut(s) | 59 |
| Ppu21I | YACGTR | 1 cut(s) | 50 |
| PpuMI | RGGWCCY | 1 cut(s) | 163 |
| Psp5II | RGGWCCY | 1 cut(s) | 163 |
| PspN4I | GGNNCC | 1 cut(s) | 165 |
| PspPI | GGNCC | 1 cut(s) | 163 |
| PspPPI | RGGWCCY | 1 cut(s) | 163 |
| RseI | CAYNNNNRTG | 1 cut(s) | 239 |
| SatI | GCNGC | 1 cut(s) | 58 |
| Sau96I | GGNCC | 1 cut(s) | 163 |
| SetI | ASST | 3 cut(s) | 52, 121, 165 |
| SinI | GGWCC | 1 cut(s) | 163 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 239 |
| SnaBI | TACGTA | 1 cut(s) | 50 |
| Sse9I | AATT | 2 cut(s) | 308, 328 |
| SspMI | CTAG | 1 cut(s) | 116 |
| TaaI | ACNGT | 1 cut(s) | 140 |
| TaiI | ACGT | 1 cut(s) | 52 |
| TaqI | TCGA | 1 cut(s) | 277 |
| TaqII | GACCGA | 1 cut(s) | 263 |
| TasI | AATT | 2 cut(s) | 308, 328 |
| TfiI | GAWTC | 1 cut(s) | 340 |
| TseFI | GTSAC | 1 cut(s) | 134 |
| TseI | GCWGC | 1 cut(s) | 57 |
| Tsp45I | GTSAC | 1 cut(s) | 134 |
| TspDTI | ATGAA | 3 cut(s) | 50, 213, 314 |
| VpaK11BI | GGWCC | 1 cut(s) | 163 |
| XspI | CTAG | 1 cut(s) | 116 |