Rw1G034750
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000261
Physical Location & Seq
Forward (+)
62654374 .. 62659478
5105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034750.1

Sequence Viewer

Length: 663 bp
ATGTTGCTCTTATTGGTGGAGTTGTGGCACCTAGAGACAATGTGTGCGTGTACTATGCTTGTGCTACTGGTGTTTAATTTGAGACGAAGACGTGCTGAAAGACATGCTCTAAATAATCGTTCATTCGTTAGACGTGAGACTCGTTTGAGTTATCTAGATAGTATTATAGGCAATAGTGACACCGAATGCGTACACGAATTAAGAATGGATAGAAGGACTTTTGGCCTTTTTGACCGTGCGATTGGAATTGGAGCTGAGGTCCCTGCTGATATGATGGAGGAGCAGAGCAACAATGAAGATGGCATTGAAGTTGAGAATGACACAAGCCCCATGTCAATGAGCACAGGACAATCTCAGGTTAGTCAGAAAAAGAGGAAAAGAAATGATGAAAATAAAATTATGATTGCATTGGATAAATTGTTTGAAGAATCTGGAAAAAGAATGCAAGCAGTGACTGATGCTATAGTGAAAGGTAATGAAGATCGATCTGATATTGCCAAGGAACTTAAGAAAATGGGACTTTCTGTTATAGACCAAATTGAGGCATTGAAAATCATTTTGGATAAGCCCCAAAATATCTCTGTGTTCATGTCCTTAGATGATGAAGTGAGAAAAGTGTATGTGGATAACTTTCTTGCGGGCACTGCTAGAGGATCTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

220

Amino Acids

25.09

Weight (kDa)

5.56

Isoelectric Point (pI)

63.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18411 FvH4_1g22560 FvH4_1g24290 FvH4_1g24290 FvH4_1g24290 FvH4_1g29434 FvH4_2g04031 FvH4_2g04811 FvH4_2g07610 FvH4_2g09412 FvH4_3g15552 FvH4_3g30720 FvH4_4g05810 FvH4_4g09541 FvH4_4g15171 FvH4_6g09145 FvH4_6g49541 FvH4_7g00320 FvH4_7g11552
malus_domestica MD00G1072400.v1.1 MD01G1188100.v1.1 MD07G1132000.v1.1 MD08G1190700.v1.1 MD10G1011200.v1.1 MD14G1164100.v1.1 MD15G1289200.v1.1 MD15G1289300.v1.1 MD17G1245100.v1.1
prunus_persica Prupe.6G228600_v2.0.a1
pyrus_communis pycom01g07070 pycom02g06890 pycom02g06900 pycom02g13930 pycom04g07470 pycom04g11770 pycom04g21930 pycom05g08520 pycom06g04490 pycom06g09560 pycom07g03750 pycom08g08760 pycom08g13660 pycom09g11520 pycom09g14250 pycom09g14770 pycom10g00720 pycom10g01290 pycom10g10480 pycom10g15700 pycom12420g00130 pycom12g05380 pycom12g08750 pycom13g19010 pycom13g29080 pycom14g06210 pycom14g13680 pycom14g19850 pycom15g22070 pycom15g23440 pycom15g24950 pycom15g25280 pycom16g11100 pycom16g17080 pycom16g26310 pycom17g15650 pycom17g15670 pycom17g20370
rosa_chinensis RchiOBHm_Chr1g0373591 RchiOBHm_Chr2g0105551 RchiOBHm_Chr2g0106711 RchiOBHm_Chr2g0106721 RchiOBHm_Chr7g0219611
rosa_laevigata RLG00000013773 RLG00000017436 RLG00000017533
rosa_multiflora Rmu_co8001842.1_g000001 Rmu_co8158450.1_g000001 Rmu_sc0000516.1_g000067 Rmu_sc0000566.1_g000009 Rmu_sc0000965.1_g000025 Rmu_sc0001718.1_g000001 Rmu_sc0001719.1_g000020 Rmu_sc0001822.1_g000025 Rmu_sc0002219.1_g000003 Rmu_sc0002460.1_g000059 Rmu_sc0002757.1_g000001 Rmu_sc0002860.1_g000001 Rmu_sc0004531.1_g000001 Rmu_sc0005558.1_g000003 Rmu_sc0011218.1_g000001 Rmu_sc0011998.1_g000003 Rmu_sc0015560.1_g000002 Rmu_sc0015560.1_g000003
rosa_roxburghii Rroxscaffold_1G00004200 Rroxscaffold_1G00026570 Rroxscaffold_1G00040370 Rroxscaffold_1G00044120 Rroxscaffold_1G00049590 Rroxscaffold_1G00059030 Rroxscaffold_2G00108440 Rroxscaffold_2G00108850 Rroxscaffold_2G00130680 Rroxscaffold_2G00132980 Rroxscaffold_2G00136860 Rroxscaffold_3G00218610 Rroxscaffold_4G00309940 Rroxscaffold_6G00405560
rosa_rugosa Rorug02G0138000 Rorug02G0138100 Rorug02G0138100 Rorug06G0154000
rosa_samantha Rh1AG121500 Rh1CG116300 Rh2AG189000 Rh2AG509600 Rh2BG200200 Rh2CG194100 Rh2DG195300 Rh5AG114400 Rh5DG488800
rosa_wichuraiana Rw0G022020 Rw1G023650 Rw1G026380 Rw1G034750 Rw1G040770 Rw2G014870 Rw2G016560 Rw4G031190 Rw5G026570 Rw6G003020 Rw6G012060 Rw7G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 27
AciI CCGC 1 cut(s) 638
AclWI GGATC 1 cut(s) 661
AfaI GTAC 2 cut(s) 52, 192
AfiI CCNNNNNNNGG 1 cut(s) 541
AflII CTTAAG 1 cut(s) 506
AgsI TTSAA 3 cut(s) 308, 425, 550
AjiI CACGTC 2 cut(s) 92, 134
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
Alw21I GWGCWC 1 cut(s) 344
Alw26I GTCTC 3 cut(s) 29, 76, 131
AlwI GGATC 1 cut(s) 661
AlwNI CAGNNNCTG 1 cut(s) 455
AoxI GGCC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 259
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 344
BbvCI CCTCAGC 1 cut(s) 255
BccI CCATC 2 cut(s) 268, 293
BcoDI GTCTC 3 cut(s) 29, 76, 131
BfaI CTAG 3 cut(s) 32, 155, 648
BfmI CTRYAG 1 cut(s) 462
BfrI CTTAAG 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 259
BmgBI CACGTC 2 cut(s) 92, 134
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 2 cut(s) 29, 261
BmsI GCATC 1 cut(s) 448
BpiI GAAGAC 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 1 cut(s) 72
BseCI ATCGAT 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMII CTCAG 2 cut(s) 246, 368
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 644
BshFI GGCC 1 cut(s) 225
BshNI GGYRCC 1 cut(s) 27
BshVI ATCGAT 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 344
BslFI GGGAC 2 cut(s) 245, 531
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 3 cut(s) 29, 76, 131
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 245, 531
BsmI GAATGC 2 cut(s) 191, 447
BsnI GGCC 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 344, 644
Bsp143I GATC 3 cut(s) 481, 485, 653
BspACI CCGC 1 cut(s) 638
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 247, 367
BspDI ATCGAT 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 29, 261
BspPI GGATC 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 27
BspTI CTTAAG 1 cut(s) 506
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 481, 485, 653
BssT1I CCWWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 236
BstAFI CTTAAG 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 447, 640
BstDEI CTNAG 3 cut(s) 255, 354, 595
BstKTI GATC 3 cut(s) 484, 488, 656
BstMAI GTCTC 3 cut(s) 29, 76, 131
BstMBI GATC 3 cut(s) 481, 485, 653
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 462
BstSLI GKGCMC 1 cut(s) 644
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 653
BstYI RGATCY 1 cut(s) 653
Bsu15I ATCGAT 1 cut(s) 484
BsuRI GGCC 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 484
BtrI CACGTC 2 cut(s) 92, 134
BtsI GCAGTG 2 cut(s) 456, 642
BtsIMutI CAGTG 2 cut(s) 456, 642
Cac8I GCNNGC 2 cut(s) 447, 640
CaiI CAGNNNCTG 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 259
ClaI ATCGAT 1 cut(s) 484
Csp6I GTAC 2 cut(s) 51, 191
CviAII CATG 3 cut(s) 104, 331, 589
CviJI RGCY 4 cut(s) 225, 254, 327, 568
CviKI_1 RGCY 4 cut(s) 225, 254, 327, 568
CviQI GTAC 2 cut(s) 51, 191
DdeI CTNAG 3 cut(s) 255, 354, 595
DpnI GATC 3 cut(s) 483, 487, 655
DpnII GATC 3 cut(s) 481, 485, 653
Eco130I CCWWGG 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 3 cut(s) 107, 334, 592
FaqI GGGAC 2 cut(s) 245, 531
FatI CATG 3 cut(s) 103, 330, 588
FauI CCCGC 1 cut(s) 631
FspBI CTAG 3 cut(s) 32, 155, 648
HaeIII GGCC 1 cut(s) 225
Hin1II CATG 3 cut(s) 107, 334, 592
HinfI GANTC 2 cut(s) 139, 428
Hpy166II GTNNAC 2 cut(s) 51, 193
Hpy188I TCNGA 2 cut(s) 366, 490
Hpy188III TCNNGA 2 cut(s) 155, 432
Hpy8I GTNNAC 2 cut(s) 51, 193
HpyAV CCTTC 1 cut(s) 207
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 2 cut(s) 91, 133
HpyCH4V TGCA 2 cut(s) 407, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 3 cut(s) 255, 354, 595
HpySE526I ACGT 2 cut(s) 91, 133
Hsp92II CATG 3 cut(s) 107, 334, 592
Kzo9I GATC 3 cut(s) 481, 485, 653
LmnI GCTCC 2 cut(s) 251, 280
LpnPI CCDG 5 cut(s) 53, 276, 330, 341, 417
LweI GCATC 1 cut(s) 448
MaeI CTAG 3 cut(s) 32, 155, 648
MaeII ACGT 2 cut(s) 91, 133
MaeIII GTNAC 2 cut(s) 176, 451
MalI GATC 3 cut(s) 483, 487, 655
MboI GATC 3 cut(s) 481, 485, 653
MboII GAAGA 4 cut(s) 99, 308, 437, 491
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 2 cut(s) 344, 644
MluCI AATT 6 cut(s) 76, 197, 246, 396, 416, 537
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 5 cut(s) 250, 271, 366, 535, 644
MseI TTAA 3 cut(s) 75, 200, 507
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 506
Mva1269I GAATGC 2 cut(s) 191, 447
MwoI GCNNNNNNNGC 1 cut(s) 644
NdeII GATC 3 cut(s) 481, 485, 653
NlaIII CATG 3 cut(s) 107, 334, 592
NlaIV GGNNCC 2 cut(s) 29, 261
NmuCI GTSAC 2 cut(s) 176, 451
NspI RCATGY 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PctI GAATGC 2 cut(s) 191, 447
PfeI GAWTC 1 cut(s) 428
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 29, 261
PspPI GGNCC 1 cut(s) 259
PspPPI RGGWCCY 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 1 cut(s) 653
RsaI GTAC 2 cut(s) 52, 192
RsaNI GTAC 2 cut(s) 51, 191
RseI CAYNNNNRTG 1 cut(s) 335
SaqAI TTAA 3 cut(s) 75, 200, 507
Sau3AI GATC 3 cut(s) 481, 485, 653
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 1 cut(s) 133
SduI GDGCHC 2 cut(s) 344, 644
SetI ASST 8 cut(s) 33, 94, 136, 256, 261, 360, 475, 662
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 1 cut(s) 462
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 6 cut(s) 76, 197, 246, 396, 416, 537
SsiI CCGC 1 cut(s) 638
SspMI CTAG 3 cut(s) 32, 155, 648
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 2 cut(s) 94, 136
TaqI TCGA 1 cut(s) 484
TasI AATT 6 cut(s) 76, 197, 246, 396, 416, 537
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 3 cut(s) 75, 200, 507
Tru9I TTAA 3 cut(s) 75, 200, 507
TscAI CASTG 2 cut(s) 456, 649
TseFI GTSAC 2 cut(s) 176, 451
Tsp45I GTSAC 2 cut(s) 176, 451
TspDTI ATGAA 6 cut(s) 111, 309, 402, 492, 577, 618
TspRI CASTG 2 cut(s) 456, 649
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 259
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 107
XspI CTAG 3 cut(s) 32, 155, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.