FvH4_3g36690

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000175
Physical Location & Seq
Forward (+)
31444496 .. 31448180
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36690.t3

Sequence Viewer

Length: 2100 bp
ATGCATACACTTCTTGAGAGTTTTTCTGTTCCCATGGAATCATTCATATTTGATCAGGGCTCAGTTTCAAACTATTCTTATCCAGATCATGGTAATGGGTTTGAAGTAAATCATGAACTCGTCAACCCAGTTTTCCATCCAACCAACTTGGACCTCCCTAGTGATTCTAGTACATCTTTTAACCCGGCTTCAGACGGATATTCTGGTGACAGTAATGACTACAATAATCCTGTTCTTAAGTACATAAGTGATATTCTTCTTGAAGAAGACATTGAGGATAAGCCCTGTATGTTGCAGGACTGTTTGGCCCTCCAAGCTGCAGAGAAATCATTCTATGATGTCCTTAATCAAAAGGATTCTACAACACTCCATCAAAGCTTTGAGTACTCGGATGATTACAGGAATAGTTGTTTTAGCAATGGTGGCGCTATTCTTGGCAAGACATACGGGGTTGCTGATCCTTCAGAAACATGTCATGTGCAATCTTCTCTTGTTCAATGTCTGTCGGATACCTCTTTGGTTACTGATACACTTTCAAAGCTGCATAGTCTTGGGGATTCTGGAAGGGTGGAGGAAGCAAATAAGTTGGTTGAAAATTTTAAAATCATTGATGCAGAGAAGTACCAATTAATGCCTCGAGGTTCTCAGACACTTGTAGGATCAAATTCAGCGTCTCAGCAACAGAATGATGGGTACAACTCAGCATATAGATCAGAGGCAAAGAAAAATCGTCAAAGGCAGGATAGTCATTGTCCAGAAGAATGGAGAAGCAACAAGCAGTCAGTTGCATATTGTGCTGATGATTCTGAGCCACAGGACATGTTTGATAAGGCGTTGCTCTTTCAAGGTGTGAATCATGAGTCCGCTGAGTCTATTTCTCATCATGATTGTTCTAATGTGGGAAGCAGGCAGCCCAAAGGATCTAAGTCAGCGCGTTCAAAGAGACAAAATAAAAAGGAGAAAGTAGTAGATTTGTGCACAATGTTAACTCAGTGCGCCCAAGCTGTTGCAAGTTATGACCAACGAAATGCTGTTGAACTACTGAAGCAGATCAGGCAGCACTCTTCTCCCTATGGTGATGCAACCCAGAGATTAGCTCATTACTTTGCTGATGGCCTTGAGGTACGCTTAGCTGGCGCTAGAACCCCGTCATATTCGCCTATTGCAAGTATGCAGATATCAACTGTTGAAATACTGAAAAGCTACCAGATGTATGTCACTTCAACCCCTCTCAAGACTGTGCCAATTTTTTTTGCCAATATGACAATTATGAAACTAGCAAGAAAAGCGACAAGTCTTCACATTATTGATTTTGGTACTTCTTATGGTTTCCAATGGCCCTCTCTAATCCAATGTCTTTCAGAGAGATATGGTGGACCTCCTAATCTCCGTATCACTACGATTGAGCTTCCCCAACCAGGTTTTAGACCCACGGAGAGGATTGAAGAGACCGGTCGTCGCTTAGCAAAGTATTGTGATAGATTTAGTGTCCCATTTGAGTTCCATGTCATTGCTCAGAAATGGGAAACTGTAGGATTTGATGATCTTAAAATTGACAGAAATGAGCTGATTGTGGTCAACTGTCTACACCGGTTGAAGCACGTTCCTGATGTGACAATGATGGGGAAGAGTCCAAGAGATGCTGTCCTAAAGTTGATCAGGAAAATCAATCCAGAAATTTTCATTCATGGGGTTGTCAATGGGGCATATAATTCACCCTTCTTTCTCACACGTTTCAAACAGGCACTCTCTCACTTTGAGGCAATCTTTGATGTCTTTGAGGCCACCATTCCTTGTGAGGATCAACAACGAATGTTTTTTGAAAGAGCAGGATATGGTAGAGACATAATGAATGTAGTGGCATGTGAGGGACTGGAAATAGTTGAAAGGCCTGAGACATATCAGAAGTGGCAGGTCAGGAATGTGAGGGCTGGGCTGAAGCAGCTCCCATTAGACCAGGAGCTGTTGAAGAAGGTGAAGACTATGTCAAAGTCGATGGATTATCACAGTGATTTTAGGATTGATGAAGATGAGCAATGGATGCTGCAGGGTTGGAAAGGAAGAGTCCTCATGGCCCTTTCCTTGTGGAAACGCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

700

Amino Acids

79.35

Weight (kDa)

5.85

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 327 - 697 3.2e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 636
Acc36I ACCTGC 1 cut(s) 1903
AccB7I CCANNNNNTGG 1 cut(s) 89
AccI GTMKAC 1 cut(s) 1585
AccII CGCG 2 cut(s) 934, 2094
AciI CCGC 1 cut(s) 864
AclWI GGATC 4 cut(s) 452, 667, 928, 1809
AcsI RAATTY 3 cut(s) 595, 664, 1677
AcuI CTGAAG 4 cut(s) 174, 447, 1064, 1958
AfaI GTAC 7 cut(s) 172, 242, 386, 623, 695, 1125, 1318
AfiI CCNNNNNNNGG 3 cut(s) 89, 1418, 1437
AflII CTTAAG 1 cut(s) 236
AflIII ACRYGT 3 cut(s) 470, 819, 1730
AgeI ACCGGT 2 cut(s) 1451, 1590
AhdI GACNNNNNGTC 1 cut(s) 1455
AjnI CCWGG 2 cut(s) 1417, 1956
Alw21I GWGCWC 1 cut(s) 980
Alw26I GTCTC 5 cut(s) 678, 937, 1442, 1836, 1889
Alw44I GTGCAC 1 cut(s) 976
AlwI GGATC 4 cut(s) 452, 667, 928, 1809
AlwNI CAGNNNCTG 1 cut(s) 1963
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 6 cut(s) 306, 1114, 1337, 1782, 1889, 2073
ApaLI GTGCAC 1 cut(s) 976
ApeKI GCWGC 6 cut(s) 317, 541, 910, 1057, 1942, 2044
ApoI RAATTY 3 cut(s) 595, 664, 1677
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AseI ATTAAT 1 cut(s) 629
AsiGI ACCGGT 2 cut(s) 1451, 1590
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 4 cut(s) 428, 934, 998, 1139
AspS9I GGNCC 5 cut(s) 151, 307, 1338, 1376, 2074
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 4 cut(s) 218, 1088, 1707, 1987
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 2 cut(s) 151, 1376
BaeGI GKGCMC 1 cut(s) 980
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 3 cut(s) 273, 1289, 1985
Bbv12I GWGCWC 1 cut(s) 980
BbvI GCAGC 6 cut(s) 304, 528, 922, 1069, 1954, 2031
BccI CCATC 6 cut(s) 144, 378, 683, 1106, 1615, 1990
BciT130I CCWGG 2 cut(s) 1419, 1958
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 2 cut(s) 52, 1656
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 5 cut(s) 678, 937, 1442, 1836, 1889
BfaI CTAG 4 cut(s) 159, 168, 1140, 1277
BfmI CTRYAG 3 cut(s) 318, 1530, 2045
BfoI RGCGCY 2 cut(s) 429, 1140
BfrI CTTAAG 1 cut(s) 236
BfuAI ACCTGC 1 cut(s) 1903
BfuI GTATCC 1 cut(s) 502
BisI GCNGC 6 cut(s) 318, 542, 911, 1058, 1943, 2045
BlpI GCTNAGC 2 cut(s) 1129, 1462
BlsI GCNGC 6 cut(s) 319, 543, 912, 1059, 1944, 2046
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 3 cut(s) 185, 1419, 1958
Bme18I GGWCC 2 cut(s) 151, 1376
BmeRI GACNNNNNGTC 1 cut(s) 1455
BmeT110I CYCGRG 1 cut(s) 636
BmgT120I GGNCC 5 cut(s) 151, 307, 1338, 1376, 2074
BmrFI CCNGG 3 cut(s) 185, 1419, 1958
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 4 cut(s) 601, 1069, 1630, 2031
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 3 cut(s) 273, 1289, 1985
BplI GAGNNNNNCTC 2 cut(s) 1081, 1113
Bpu1102I GCTNAGC 2 cut(s) 1129, 1462
BpuEI CTTGAG 3 cut(s) 35, 1139, 1217
BpuMI CCSGG 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 1442
BsaJI CCNNGG 2 cut(s) 33, 1431
BsaWI WCCGGW 2 cut(s) 1451, 1590
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 1418, 1437
Bse118I RCCGGY 2 cut(s) 1451, 1590
Bse1I ACTGG 2 cut(s) 128, 1878
Bse3DI GCAATG 3 cut(s) 424, 1509, 2042
BseBI CCWGG 2 cut(s) 1419, 1958
BseDI CCNNGG 2 cut(s) 33, 1431
BseGI GGATG 3 cut(s) 136, 397, 2046
BseLI CCNNNNNNNGG 3 cut(s) 89, 1418, 1437
BseMI GCAATG 3 cut(s) 424, 1509, 2042
BseMII CTCAG 9 cut(s) 75, 659, 689, 714, 798, 858, 1004, 1529, 1884
BseNI ACTGG 2 cut(s) 128, 1878
BseSI GKGCMC 1 cut(s) 980
BseXI GCAGC 6 cut(s) 304, 528, 922, 1069, 1954, 2031
BseYI CCCAGC 1 cut(s) 1931
Bsh1236I CGCG 2 cut(s) 934, 2094
Bsh1285I CGRYCG 1 cut(s) 1456
BshFI GGCC 6 cut(s) 308, 1116, 1339, 1784, 1891, 2075
BshTI ACCGGT 2 cut(s) 1451, 1590
BsiEI CGRYCG 1 cut(s) 1456
BsiHKAI GWGCWC 1 cut(s) 980
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 3 cut(s) 185, 1452, 1591
BslFI GGGAC 2 cut(s) 1475, 1884
BslI CCNNNNNNNGG 3 cut(s) 89, 1418, 1437
BsmAI GTCTC 5 cut(s) 678, 937, 1442, 1836, 1889
BsmBI CGTCTC 1 cut(s) 678
BsmFI GGGAC 2 cut(s) 1475, 1884
BsnI GGCC 6 cut(s) 308, 1116, 1339, 1784, 1891, 2075
Bso31I GGTCTC 1 cut(s) 1442
BsoBI CYCGRG 1 cut(s) 636
Bsp1286I GDGCHC 2 cut(s) 62, 980
Bsp1720I GCTNAGC 2 cut(s) 1129, 1462
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 1 cut(s) 864
BspANI GGCC 6 cut(s) 308, 1116, 1339, 1784, 1891, 2075
BspCNI CTCAG 9 cut(s) 74, 658, 688, 713, 799, 859, 1003, 1528, 1885
BspFNI CGCG 2 cut(s) 934, 2094
BspHI TCATGA 3 cut(s) 112, 856, 883
BspMAI CTGCAG 2 cut(s) 322, 2049
BspMI ACCTGC 1 cut(s) 1903
BspPI GGATC 4 cut(s) 452, 667, 928, 1809
BspTI CTTAAG 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 1442
BsrDI GCAATG 3 cut(s) 424, 1509, 2042
BsrFI RCCGGY 2 cut(s) 1451, 1590
BsrI ACTGG 2 cut(s) 128, 1878
BssAI RCCGGY 2 cut(s) 1451, 1590
BssECI CCNNGG 2 cut(s) 33, 1431
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 1419, 1958
Bst4CI ACNGT 7 cut(s) 212, 302, 1186, 1240, 1531, 1583, 2009
Bst6I CTCTTC 4 cut(s) 1069, 1440, 1622, 2056
BstAFI CTTAAG 1 cut(s) 236
BstAPI GCANNNNNTGC 2 cut(s) 794, 2041
BstC8I GCNNGC 2 cut(s) 908, 1135
BstDSI CCRYGG 2 cut(s) 33, 1431
BstF5I GGATG 3 cut(s) 136, 397, 2046
BstFNI CGCG 2 cut(s) 934, 2094
BstH2I RGCGCY 2 cut(s) 429, 1140
BstHHI GCGC 4 cut(s) 428, 934, 998, 1139
BstMAI GTCTC 5 cut(s) 678, 937, 1442, 1836, 1889
BstMCI CGRYCG 1 cut(s) 1456
BstMWI GCNNNNNNNGC 8 cut(s) 314, 423, 794, 1054, 1134, 1286, 1942, 2041
BstNI CCWGG 2 cut(s) 1419, 1958
BstNSI RCATGY 3 cut(s) 474, 823, 1866
BstSCI CCNGG 3 cut(s) 183, 1417, 1956
BstSFI CTRYAG 3 cut(s) 318, 1530, 2045
BstSLI GKGCMC 1 cut(s) 980
BstUI CGCG 2 cut(s) 934, 2094
BstV1I GCAGC 6 cut(s) 304, 528, 922, 1069, 1954, 2031
BstV2I GAAGAC 3 cut(s) 273, 1289, 1985
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 762
BstYI RGATCY 1 cut(s) 920
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 6 cut(s) 308, 1116, 1339, 1784, 1891, 2075
BtgI CCRYGG 2 cut(s) 33, 1431
BtsCI GGATG 3 cut(s) 136, 397, 2046
BtsIMutI CAGTG 2 cut(s) 998, 2014
BveI ACCTGC 1 cut(s) 1903
Cac8I GCNNGC 2 cut(s) 908, 1135
CaiI CAGNNNCTG 1 cut(s) 1963
CciI TCATGA 3 cut(s) 112, 856, 883
CfoI GCGC 4 cut(s) 428, 934, 998, 1139
Cfr10I RCCGGY 2 cut(s) 1451, 1590
Cfr13I GGNCC 5 cut(s) 151, 307, 1338, 1376, 2074
CseI GACGC 1 cut(s) 660
CsiI ACCWGGT 1 cut(s) 1417
Csp6I GTAC 7 cut(s) 171, 241, 385, 622, 694, 1124, 1317
CspAI ACCGGT 2 cut(s) 1451, 1590
CviQI GTAC 7 cut(s) 171, 241, 385, 622, 694, 1124, 1317
DraI TTTAAA 1 cut(s) 601
DriI GACNNNNNGTC 1 cut(s) 1455
Eam1104I CTCTTC 4 cut(s) 1069, 1440, 1622, 2056
Eam1105I GACNNNNNGTC 1 cut(s) 1455
EarI CTCTTC 4 cut(s) 1069, 1440, 1622, 2056
Eco130I CCWWGG 1 cut(s) 33
Eco147I AGGCCT 1 cut(s) 1891
Eco24I GRGCYC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 1442
Eco32I GATATC 1 cut(s) 1179
Eco47I GGWCC 2 cut(s) 151, 1376
Eco57I CTGAAG 4 cut(s) 174, 447, 1064, 1958
Eco88I CYCGRG 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 1417, 1956
EcoRV GATATC 1 cut(s) 1179
EcoT14I CCWWGG 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 33
Esp3I CGTCTC 1 cut(s) 678
FaqI GGGAC 2 cut(s) 1475, 1884
FbaI TGATCA 2 cut(s) 52, 1656
FblI GTMKAC 1 cut(s) 1585
Fnu4HI GCNGC 6 cut(s) 318, 542, 911, 1058, 1943, 2045
FokI GGATG 3 cut(s) 123, 404, 2053
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 6 cut(s) 318, 542, 911, 1058, 1943, 2045
FspBI CTAG 4 cut(s) 159, 168, 1140, 1277
GlaI GCGC 4 cut(s) 427, 933, 997, 1138
GluI GCNGC 6 cut(s) 318, 542, 911, 1058, 1943, 2045
GsaI CCCAGC 1 cut(s) 1935
HaeII RGCGCY 2 cut(s) 429, 1140
HaeIII GGCC 6 cut(s) 308, 1116, 1339, 1784, 1891, 2075
HapII CCGG 3 cut(s) 185, 1452, 1591
HgaI GACGC 1 cut(s) 660
HhaI GCGC 4 cut(s) 428, 934, 998, 1139
Hin6I GCGC 4 cut(s) 426, 932, 996, 1137
HinP1I GCGC 4 cut(s) 426, 932, 996, 1137
HincII GTYRAC 3 cut(s) 124, 987, 1579
HindII GTYRAC 3 cut(s) 124, 987, 1579
HindIII AAGCTT 1 cut(s) 376
HpaI GTTAAC 1 cut(s) 987
HpaII CCGG 3 cut(s) 185, 1452, 1591
HphI GGTGA 4 cut(s) 218, 1088, 1707, 1987
Hpy166II GTNNAC 6 cut(s) 124, 978, 987, 1376, 1579, 1586
Hpy8I GTNNAC 6 cut(s) 124, 978, 987, 1376, 1579, 1586
Hpy99I CGWCG 1 cut(s) 1461
HpyAV CCTTC 4 cut(s) 471, 558, 1729, 1966
HpyCH4III ACNGT 7 cut(s) 212, 302, 1186, 1240, 1531, 1583, 2009
HpyCH4IV ACGT 2 cut(s) 1602, 1732
HpyF10VI GCNNNNNNNGC 8 cut(s) 314, 423, 794, 1054, 1134, 1286, 1942, 2041
HpySE526I ACGT 2 cut(s) 1602, 1732
HspAI GCGC 4 cut(s) 426, 932, 996, 1137
Ksp22I TGATCA 2 cut(s) 52, 1656
KspAI GTTAAC 1 cut(s) 987
LmnI GCTCC 2 cut(s) 1950, 1960
Lsp1109I GCAGC 6 cut(s) 304, 528, 922, 1069, 1954, 2031
LweI GCATC 4 cut(s) 601, 1069, 1630, 2031
MabI ACCWGGT 1 cut(s) 1417
MaeI CTAG 4 cut(s) 159, 168, 1140, 1277
MaeII ACGT 2 cut(s) 1602, 1732
MaeIII GTNAC 4 cut(s) 206, 520, 1216, 1612
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 2 cut(s) 62, 980
MluCI AATT 8 cut(s) 595, 626, 664, 1245, 1266, 1551, 1677, 1711
MlyI GAGTC 4 cut(s) 869, 878, 1639, 2073
MmeI TCCRAC 3 cut(s) 164, 486, 2033
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 7 cut(s) 180, 237, 345, 600, 629, 986, 1548
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 866
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 3 cut(s) 185, 1452, 1591
MspR9I CCNGG 3 cut(s) 185, 1419, 1958
MvaI CCWGG 2 cut(s) 1419, 1958
MvnI CGCG 2 cut(s) 934, 2094
MwoI GCNNNNNNNGC 8 cut(s) 314, 423, 794, 1054, 1134, 1286, 1942, 2041
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 33
NmuCI GTSAC 3 cut(s) 206, 1216, 1612
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 474, 823, 1866
PaeR7I CTCGAG 1 cut(s) 636
PagI TCATGA 3 cut(s) 112, 856, 883
PceI AGGCCT 1 cut(s) 1891
PciI ACATGT 2 cut(s) 470, 819
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 6 cut(s) 38, 164, 356, 557, 803, 853
PflFI GACNNNGTC 1 cut(s) 1984
PflMI CCANNNNNTGG 1 cut(s) 89
PinAI ACCGGT 2 cut(s) 1451, 1590
PkrI GCNGC 6 cut(s) 319, 543, 912, 1059, 1944, 2046
PleI GAGTC 4 cut(s) 868, 877, 1638, 2072
PpsI GAGTC 4 cut(s) 868, 877, 1638, 2072
PscI ACATGT 2 cut(s) 470, 819
PshBI ATTAAT 1 cut(s) 629
Psp6I CCWGG 2 cut(s) 1417, 1956
PspFI CCCAGC 1 cut(s) 1931
PspGI CCWGG 2 cut(s) 1417, 1956
PspPI GGNCC 5 cut(s) 151, 307, 1338, 1376, 2074
PspXI VCTCGAGB 1 cut(s) 636
PstI CTGCAG 2 cut(s) 322, 2049
PstNI CAGNNNCTG 1 cut(s) 1963
PsuI RGATCY 1 cut(s) 920
PsyI GACNNNGTC 1 cut(s) 1984
RsaI GTAC 7 cut(s) 172, 242, 386, 623, 695, 1125, 1318
RsaNI GTAC 7 cut(s) 171, 241, 385, 622, 694, 1124, 1317
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 7 cut(s) 180, 237, 345, 600, 629, 986, 1548
SatI GCNGC 6 cut(s) 318, 542, 911, 1058, 1943, 2045
Sau96I GGNCC 5 cut(s) 151, 307, 1338, 1376, 2074
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 4 cut(s) 869, 878, 1639, 2073
ScrFI CCNGG 3 cut(s) 185, 1419, 1958
SduI GDGCHC 2 cut(s) 62, 980
SexAI ACCWGGT 1 cut(s) 1417
SfaNI GCATC 4 cut(s) 601, 1069, 1630, 2031
SfcI CTRYAG 3 cut(s) 318, 1530, 2045
Sfr274I CTCGAG 1 cut(s) 636
SinI GGWCC 2 cut(s) 151, 1376
SlaI CTCGAG 1 cut(s) 636
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 5 cut(s) 14, 236, 636, 1118, 1232
SmoI CTYRAG 5 cut(s) 14, 236, 636, 1118, 1232
Sse9I AATT 8 cut(s) 595, 626, 664, 1245, 1266, 1551, 1677, 1711
SseBI AGGCCT 1 cut(s) 1891
SsiI CCGC 1 cut(s) 864
SspMI CTAG 4 cut(s) 159, 168, 1140, 1277
StuI AGGCCT 1 cut(s) 1891
StyD4I CCNGG 3 cut(s) 183, 1417, 1956
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 7 cut(s) 212, 302, 1186, 1240, 1531, 1583, 2009
TaiI ACGT 2 cut(s) 1605, 1735
TaqI TCGA 2 cut(s) 637, 1994
TasI AATT 8 cut(s) 595, 626, 664, 1245, 1266, 1551, 1677, 1711
TatI WGTACW 3 cut(s) 170, 240, 384
TfiI GAWTC 6 cut(s) 38, 164, 356, 557, 803, 853
Tru1I TTAA 7 cut(s) 180, 237, 345, 600, 629, 986, 1548
Tru9I TTAA 7 cut(s) 180, 237, 345, 600, 629, 986, 1548
TscAI CASTG 2 cut(s) 998, 2014
TseFI GTSAC 3 cut(s) 206, 1216, 1612
TseI GCWGC 6 cut(s) 317, 541, 910, 1057, 1942, 2044
Tsp45I GTSAC 3 cut(s) 206, 1216, 1612
TspDTI ATGAA 7 cut(s) 34, 129, 1286, 1672, 1676, 1865, 2040
TspGWI ACGGA 3 cut(s) 210, 1379, 1448
TspRI CASTG 2 cut(s) 998, 2014
Tth111I GACNNNGTC 1 cut(s) 1984
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 236
VneI GTGCAC 1 cut(s) 976
VpaK11BI GGWCC 2 cut(s) 151, 1376
VspI ATTAAT 1 cut(s) 629
XapI RAATTY 3 cut(s) 595, 664, 1677
XceI RCATGY 3 cut(s) 474, 823, 1866
XhoI CTCGAG 1 cut(s) 636
XmiI GTMKAC 1 cut(s) 1585
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 4 cut(s) 159, 168, 1140, 1277
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.