FvH4_3g36730

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000175
Physical Location & Seq
Forward (+)
31485407 .. 31487576
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36730.t1

Sequence Viewer

Length: 2028 bp
ATGGATGAATTTCCAAGTTCCTTCAATAATTTCATGTCTAACCAGAACTCAATACCAACATTTTCAGTTCAGAATACCCTTGTTACTGAATCTGAATCTGATCAACACAATCATGAACTCAGTGATCCCAGTTTCTTTCCATCCAATCCAAACCCTCCTCCTCATGACTCATCTTCATCTTTGGATTTGAGCTCTGAGGGGGAGTATTACTCTCCCTCCAGTGCTACTCTCCAATACATAAGTCAGATGCTTATGGAGGAAGAATTGGAGAACACACCCTGCATGCTTCAGGACTGTCTGGCCCTCCAAGCGGCTGAGAAATCCTTGCATGATGTCCTAATCCATCAATATCCTTCTTCGACTACTAACCTCCTTGCTTCTTCCATTCACCAAACTGCTGAGATATCTGAAGATCTCATCAACCACAGCAGTAATAGCTCCACTGCTGCTAGGGAGTGGAATGATGGTTCAGGTTGGATTTCTTTTCAAAATGTATTAGAATCCCATCCTTTGGAAAATAATACCCTTTTGGTTCCAAATCCTTTCTCCTCAAATGGAAAGGGTGGAATCTTAGATCTTGAAAGGGGCTCATCATTTCTCAAACAACCAAGGGAGGTGACTGAGGATGGGAATGACTCAACCAACAGATCAAGGAGCAAGAAAGATCGTCAGCGGGAGGATAGCAGTTATCAAGAAGAAGGGAGGAGCAACAAGCAGACAGCATTTTACGCAGCTGAGACTGAGCCACCAGAGATGTTAGATAAGGTACTGCTGTGTCAATATACAATCTCATGTTCTTCTCTTCAGGAATCTGATCAGCTTAAAAGGGCAAGTGGACATAGAGGATTCAAAGGCAAAAGAACAGGAAAAAAAATGGTGGATGACAACAGAGCAGTAGTGGATTTTCAGATACTTCTAACTCAATGTGCAAAAGCTGTTATAAGCTATGACATAAGCACTGCAAATGAAAAACTGAAGCTGATAAGGCAGCACTCATCTCCCCATGGTGATGGAACACAGCGATTAGCTCATTACTTAGCCAATGGCCTCGAAGAACGCTTGAATGCAGCGGTCCCCTTGTACAACCCCCATTGCCTTTTCAGTTGTAAGATGTCAGCGGCTGATATCTTAAAAGCTTACCAGACTTATATGAAAGCATGCCCTTTCAAGCTGATGTCAAATATATATGCTAACAATACTATTCTCAAGCTAGCAGAGAACGTAACGAGGCTACACATAATCGATTTTGGTATTCTCTATGGCTACCAATGGCCTTGCCTCATCCAACTGCTTGCAAGAAAACCCTCCGGACCTCCCATGCTTCACATTACTGGTATTGAATTTCCCCAATCAGGACTTCAACCTTCAGGGAGGCTTGAAGAGACAGGGAACCGCCTAGCTAAATACTGCAAGAGATTCAATGTCCCATTTAAGTACAATTTCATAGCACAGAGTTGGGAAACCATTCAATATGAAGATATCAAGCTTGACAGAGATGAGTTGACTGTAGTTAACTGCTTGTACAGGTTGAAGAACTTGCCTGATGAAACAGAAATCAACAGTCCAAGGGATACAGTTTTGAAGCTTATTAGGAGAATCAACCCAGAAATGTTCATCCATGGAGTGGTTAATGGAACCTACAATGCACCCTTCTTTGATATACGGTTCCGAGAGACACTCTACCATTTCTCTTCTTTGTTTGATATGTTTGAGGAGACTTTGCCCAGAGAAGATCAACAGAGACTGCTCTATGAACAAGAGGTATTTGGTAGAGATATTATCAATGTGATAGCATGTGAGGGCTCCAGGAGGCTTGAAAAGCCTGAAACATACAAGCAGTGGCAGATAAGAAACACTAGAGCTGGGTTTAAGCAGTTACCACTAAACCAGGAAATGCTGATGAAAGTTAAGAATATTGTAAGGTTAGGTTACCATGAGGACTTTGTTGCGAACGAAGATGGGAGGTGGCTCTTGCAGGGATGGAAAGGCCGAATATTACATGCTATTTCTTGCTGGAAATCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

676

Amino Acids

77.07

Weight (kDa)

5.81

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 305 - 673 1.6e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 941
AccB7I CCANNNNNTGG 2 cut(s) 511, 1624
AccIII TCCGGA 1 cut(s) 1307
AciI CCGC 5 cut(s) 311, 673, 1070, 1118, 1393
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 2 cut(s) 8, 1340
AcuI CTGAAG 5 cut(s) 272, 429, 788, 995, 1350
AfaI GTAC 4 cut(s) 768, 1082, 1436, 1523
AfiI CCNNNNNNNGG 4 cut(s) 310, 511, 1352, 1624
AjnI CCWGG 2 cut(s) 1805, 1887
AjuI GAANNNNNNNTTGG 2 cut(s) 1341, 1373
AloI GAACNNNNNNTCC 2 cut(s) 451, 483
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 5 cut(s) 731, 1376, 1667, 1709, 1735
AlwI GGATC 1 cut(s) 119
AlwNI CAGNNNCTG 2 cut(s) 1121, 1744
Aor13HI TCCGGA 1 cut(s) 1307
AoxI GGCC 4 cut(s) 300, 1045, 1271, 1987
ApeKI GCWGC 4 cut(s) 446, 731, 988, 1067
ApoI RAATTY 2 cut(s) 8, 1340
Asp700I GAANNNNTTC 1 cut(s) 1464
AspS9I GGNCC 3 cut(s) 301, 1072, 1310
AsuHPI GGTGA 3 cut(s) 380, 628, 1019
AsuNHI GCTAGC 1 cut(s) 1210
AvaII GGWCC 2 cut(s) 1072, 1310
BaeI ACNNNNGTAYC 2 cut(s) 758, 791
BanII GRGCYC 3 cut(s) 194, 590, 1805
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 4 cut(s) 433, 743, 1000, 1079
BccI CCATC 8 cut(s) 148, 351, 458, 513, 620, 1004, 1952, 1974
BciT130I CCWGG 2 cut(s) 1807, 1889
BciVI GTATCC 1 cut(s) 1564
BclI TGATCA 2 cut(s) 100, 814
BcoDI GTCTC 5 cut(s) 731, 1376, 1667, 1709, 1735
BfaI CTAG 4 cut(s) 450, 1211, 1397, 1857
BfmI CTRYAG 1 cut(s) 1506
BfuI GTATCC 1 cut(s) 1564
BglII AGATCT 2 cut(s) 412, 574
BisI GCNGC 6 cut(s) 312, 447, 732, 989, 1068, 1119
BlsI GCNGC 6 cut(s) 313, 448, 733, 990, 1069, 1120
Bme1390I CCNGG 2 cut(s) 1807, 1889
Bme18I GGWCC 2 cut(s) 1072, 1310
BmgT120I GGNCC 3 cut(s) 301, 1072, 1310
BmiI GGNNCC 6 cut(s) 534, 1074, 1391, 1636, 1667, 1804
BmrFI CCNGG 2 cut(s) 1807, 1889
BmrI ACTGGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 237
BmtI GCTAGC 1 cut(s) 1214
BmuI ACTGGG 1 cut(s) 123
BplI GAGNNNNNCTC 4 cut(s) 194, 226, 1662, 1694
BpmI CTGGAG 2 cut(s) 202, 1789
BpuEI CTTGAG 1 cut(s) 1190
Bsa29I ATCGAT 1 cut(s) 1242
BsaJI CCNNGG 4 cut(s) 608, 1003, 1565, 1618
BsaWI WCCGGW 1 cut(s) 1307
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 511, 1352, 1624
Bse1I ACTGG 3 cut(s) 129, 219, 1336
Bse3DI GCAATG 1 cut(s) 1090
BseAI TCCGGA 1 cut(s) 1307
BseBI CCWGG 2 cut(s) 1807, 1889
BseCI ATCGAT 1 cut(s) 1242
BseDI CCNNGG 4 cut(s) 608, 1003, 1565, 1618
BseGI GGATG 8 cut(s) 10, 140, 505, 631, 886, 1281, 1614, 1985
BseLI CCNNNNNNNGG 4 cut(s) 310, 511, 1352, 1624
BseMI GCAATG 1 cut(s) 1090
BseMII CTCAG 7 cut(s) 133, 186, 306, 390, 612, 726, 732
BseNI ACTGG 3 cut(s) 129, 219, 1336
BseRI GAGGAG 5 cut(s) 147, 150, 538, 718, 1727
BseXI GCAGC 4 cut(s) 433, 743, 1000, 1079
BseYI CCCAGC 1 cut(s) 1862
BshFI GGCC 4 cut(s) 302, 1047, 1273, 1989
BshVI ATCGAT 1 cut(s) 1242
BsiHKAI GWGCWC 1 cut(s) 194
BsiSI CCGG 1 cut(s) 1308
BslFI GGGAC 2 cut(s) 1058, 1409
BslI CCNNNNNNNGG 4 cut(s) 310, 511, 1352, 1624
BsmAI GTCTC 5 cut(s) 731, 1376, 1667, 1709, 1735
BsmFI GGGAC 2 cut(s) 1058, 1409
BsmI GAATGC 1 cut(s) 1069
BsnI GGCC 4 cut(s) 302, 1047, 1273, 1989
Bsp1286I GDGCHC 3 cut(s) 194, 590, 1805
Bsp13I TCCGGA 1 cut(s) 1307
Bsp1407I TGTACA 2 cut(s) 1080, 1521
Bsp143I GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
Bsp19I CCATGG 2 cut(s) 1003, 1618
BspACI CCGC 5 cut(s) 311, 673, 1070, 1118, 1393
BspANI GGCC 4 cut(s) 302, 1047, 1273, 1989
BspCNI CTCAG 7 cut(s) 132, 187, 307, 391, 613, 727, 733
BspDI ATCGAT 1 cut(s) 1242
BspEI TCCGGA 1 cut(s) 1307
BspHI TCATGA 2 cut(s) 112, 163
BspLI GGNNCC 6 cut(s) 534, 1074, 1391, 1636, 1667, 1804
BspOI GCTAGC 1 cut(s) 1214
BspPI GGATC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 1090
BsrGI TGTACA 2 cut(s) 1080, 1521
BsrI ACTGG 3 cut(s) 129, 219, 1336
BssECI CCNNGG 4 cut(s) 608, 1003, 1565, 1618
BssMI GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
BssT1I CCWWGG 4 cut(s) 608, 1003, 1565, 1618
Bst2UI CCWGG 2 cut(s) 1807, 1889
Bst4CI ACNGT 5 cut(s) 296, 1507, 1562, 1576, 1665
Bst6I CTCTTC 3 cut(s) 807, 1374, 1696
BstAUI TGTACA 2 cut(s) 1080, 1521
BstC8I GCNNGC 4 cut(s) 284, 1159, 1212, 1293
BstDEI CTNAG 9 cut(s) 119, 195, 315, 399, 571, 621, 735, 741, 1036
BstDSI CCRYGG 2 cut(s) 1003, 1618
BstEII GGTNACC 1 cut(s) 1928
BstF5I GGATG 8 cut(s) 10, 140, 505, 631, 886, 1281, 1614, 1985
BstKTI GATC 8 cut(s) 103, 127, 415, 577, 650, 667, 817, 1735
BstMAI GTCTC 5 cut(s) 731, 1376, 1667, 1709, 1735
BstMBI GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
BstMWI GCNNNNNNNGC 5 cut(s) 308, 435, 728, 985, 1819
BstNI CCWGG 2 cut(s) 1807, 1889
BstNSI RCATGY 4 cut(s) 286, 1161, 1797, 2003
BstPI GGTNACC 1 cut(s) 1928
BstSCI CCNGG 2 cut(s) 1805, 1887
BstSFI CTRYAG 1 cut(s) 1506
BstV1I GCAGC 4 cut(s) 433, 743, 1000, 1079
BstX2I RGATCY 2 cut(s) 412, 574
BstXI CCANNNNNNTGG 1 cut(s) 1010
BstYI RGATCY 2 cut(s) 412, 574
Bsu15I ATCGAT 1 cut(s) 1242
BsuI GTATCC 1 cut(s) 1564
BsuRI GGCC 4 cut(s) 302, 1047, 1273, 1989
BsuTUI ATCGAT 1 cut(s) 1242
BtgI CCRYGG 2 cut(s) 1003, 1618
BtsCI GGATG 8 cut(s) 10, 140, 505, 631, 886, 1281, 1614, 1985
BtsI GCAGTG 3 cut(s) 441, 957, 1844
BtsIMutI CAGTG 5 cut(s) 127, 226, 441, 957, 1844
Cac8I GCNNGC 4 cut(s) 284, 1159, 1212, 1293
CaiI CAGNNNCTG 2 cut(s) 1121, 1744
CciI TCATGA 2 cut(s) 112, 163
Cfr13I GGNCC 3 cut(s) 301, 1072, 1310
ClaI ATCGAT 1 cut(s) 1242
Csp6I GTAC 4 cut(s) 767, 1081, 1435, 1522
CviQI GTAC 4 cut(s) 767, 1081, 1435, 1522
DdeI CTNAG 9 cut(s) 119, 195, 315, 399, 571, 621, 735, 741, 1036
DpnI GATC 8 cut(s) 102, 126, 414, 576, 649, 666, 816, 1734
DpnII GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
Eam1104I CTCTTC 3 cut(s) 807, 1374, 1696
EarI CTCTTC 3 cut(s) 807, 1374, 1696
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 4 cut(s) 608, 1003, 1565, 1618
Eco24I GRGCYC 3 cut(s) 194, 590, 1805
Eco32I GATATC 3 cut(s) 405, 1126, 1480
Eco47I GGWCC 2 cut(s) 1072, 1310
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 5 cut(s) 272, 429, 788, 995, 1350
Eco91I GGTNACC 1 cut(s) 1928
EcoICRI GAGCTC 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 1928
EcoRII CCWGG 2 cut(s) 1805, 1887
EcoRV GATATC 3 cut(s) 405, 1126, 1480
EcoT14I CCWWGG 4 cut(s) 608, 1003, 1565, 1618
EcoT38I GRGCYC 3 cut(s) 194, 590, 1805
ErhI CCWWGG 4 cut(s) 608, 1003, 1565, 1618
FaqI GGGAC 2 cut(s) 1058, 1409
FauI CCCGC 1 cut(s) 666
FbaI TGATCA 2 cut(s) 100, 814
Fnu4HI GCNGC 6 cut(s) 312, 447, 732, 989, 1068, 1119
FokI GGATG 8 cut(s) 17, 127, 492, 638, 893, 1268, 1601, 1992
FriOI GRGCYC 3 cut(s) 194, 590, 1805
Fsp4HI GCNGC 6 cut(s) 312, 447, 732, 989, 1068, 1119
FspBI CTAG 4 cut(s) 450, 1211, 1397, 1857
GluI GCNGC 6 cut(s) 312, 447, 732, 989, 1068, 1119
GsaI CCCAGC 1 cut(s) 1866
GsuI CTGGAG 2 cut(s) 202, 1789
HaeIII GGCC 4 cut(s) 302, 1047, 1273, 1989
HapII CCGG 1 cut(s) 1308
HincII GTYRAC 2 cut(s) 1503, 1513
HindII GTYRAC 2 cut(s) 1503, 1513
HindIII AAGCTT 3 cut(s) 1134, 1484, 1583
HpaI GTTAAC 1 cut(s) 1513
HpaII CCGG 1 cut(s) 1308
HphI GGTGA 3 cut(s) 380, 628, 1019
Hpy166II GTNNAC 3 cut(s) 836, 1503, 1513
Hpy188I TCNGA 9 cut(s) 72, 94, 100, 196, 246, 409, 814, 909, 1670
Hpy188III TCNNGA 8 cut(s) 113, 164, 290, 578, 692, 806, 1308, 1353
Hpy8I GTNNAC 3 cut(s) 836, 1503, 1513
HpyAV CCTTC 5 cut(s) 31, 363, 692, 1374, 1660
HpyCH4III ACNGT 5 cut(s) 296, 1507, 1562, 1576, 1665
HpyCH4IV ACGT 1 cut(s) 1221
HpyCH4V TGCA 9 cut(s) 282, 328, 929, 962, 1067, 1295, 1410, 1646, 1975
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 435, 728, 985, 1819
HpyF3I CTNAG 9 cut(s) 119, 195, 315, 399, 571, 621, 735, 741, 1036
HpySE526I ACGT 1 cut(s) 1221
Kpn2I TCCGGA 1 cut(s) 1307
Ksp22I TGATCA 2 cut(s) 100, 814
KspAI GTTAAC 1 cut(s) 1513
Kzo9I GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
LmnI GCTCC 4 cut(s) 443, 654, 705, 1808
Lsp1109I GCAGC 4 cut(s) 433, 743, 1000, 1079
LweI GCATC 1 cut(s) 237
MaeI CTAG 4 cut(s) 450, 1211, 1397, 1857
MaeII ACGT 1 cut(s) 1221
MaeIII GTNAC 5 cut(s) 82, 616, 1222, 1875, 1928
MalI GATC 8 cut(s) 102, 126, 414, 576, 649, 666, 816, 1734
MboI GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
MflI RGATCY 2 cut(s) 412, 574
MhlI GDGCHC 3 cut(s) 194, 590, 1805
MluCI AATT 5 cut(s) 8, 28, 263, 1340, 1438
MlyI GAGTC 2 cut(s) 161, 629
MmeI TCCRAC 2 cut(s) 455, 1309
MroI TCCGGA 1 cut(s) 1307
MroXI GAANNNNTTC 1 cut(s) 1464
MseI TTAA 7 cut(s) 822, 1130, 1431, 1512, 1629, 1869, 1908
MslI CAYNNNNRTG 2 cut(s) 111, 1008
MspA1I CMGCKG 4 cut(s) 673, 734, 1070, 1118
MspI CCGG 1 cut(s) 1308
MspR9I CCNGG 2 cut(s) 1807, 1889
Mva1269I GAATGC 1 cut(s) 1069
MvaI CCWGG 2 cut(s) 1807, 1889
MwoI GCNNNNNNNGC 5 cut(s) 308, 435, 728, 985, 1819
NcoI CCATGG 2 cut(s) 1003, 1618
NdeII GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
NheI GCTAGC 1 cut(s) 1210
NlaIV GGNNCC 6 cut(s) 534, 1074, 1391, 1636, 1667, 1804
NmuCI GTSAC 1 cut(s) 616
NspI RCATGY 4 cut(s) 286, 1161, 1797, 2003
PaeI GCATGC 2 cut(s) 286, 1161
PagI TCATGA 2 cut(s) 112, 163
PctI GAATGC 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 1464
PfeI GAWTC 8 cut(s) 89, 95, 500, 567, 809, 846, 1416, 1596
PflMI CCANNNNNTGG 2 cut(s) 511, 1624
PfoI TCCNGGA 1 cut(s) 1805
PkrI GCNGC 6 cut(s) 313, 448, 733, 990, 1069, 1120
PleI GAGTC 2 cut(s) 161, 629
PpsI GAGTC 2 cut(s) 161, 629
PsiI TTATAA 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 1805, 1887
PspEI GGTNACC 1 cut(s) 1928
PspFI CCCAGC 1 cut(s) 1862
PspGI CCWGG 2 cut(s) 1805, 1887
PspN4I GGNNCC 6 cut(s) 534, 1074, 1391, 1636, 1667, 1804
PspPI GGNCC 3 cut(s) 301, 1072, 1310
PstNI CAGNNNCTG 2 cut(s) 1121, 1744
PsuI RGATCY 2 cut(s) 412, 574
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 4 cut(s) 768, 1082, 1436, 1523
RsaNI GTAC 4 cut(s) 767, 1081, 1435, 1522
RseI CAYNNNNRTG 2 cut(s) 111, 1008
SacI GAGCTC 1 cut(s) 194
SaqAI TTAA 7 cut(s) 822, 1130, 1431, 1512, 1629, 1869, 1908
SatI GCNGC 6 cut(s) 312, 447, 732, 989, 1068, 1119
Sau3AI GATC 8 cut(s) 100, 124, 412, 574, 647, 664, 814, 1732
Sau96I GGNCC 3 cut(s) 301, 1072, 1310
SchI GAGTC 2 cut(s) 161, 629
ScrFI CCNGG 2 cut(s) 1807, 1889
SduI GDGCHC 3 cut(s) 194, 590, 1805
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 1506
SinI GGWCC 2 cut(s) 1072, 1310
SmiMI CAYNNNNRTG 2 cut(s) 111, 1008
SmlI CTYRAG 1 cut(s) 1205
SmoI CTYRAG 1 cut(s) 1205
SphI GCATGC 2 cut(s) 286, 1161
Sse9I AATT 5 cut(s) 8, 28, 263, 1340, 1438
SsiI CCGC 5 cut(s) 311, 673, 1070, 1118, 1393
SspI AATATT 2 cut(s) 1915, 1995
SspMI CTAG 4 cut(s) 450, 1211, 1397, 1857
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 1805, 1887
StyI CCWWGG 4 cut(s) 608, 1003, 1565, 1618
TaaI ACNGT 5 cut(s) 296, 1507, 1562, 1576, 1665
TaiI ACGT 1 cut(s) 1224
TaqI TCGA 3 cut(s) 359, 1050, 1242
TasI AATT 5 cut(s) 8, 28, 263, 1340, 1438
TatI WGTACW 3 cut(s) 1080, 1434, 1521
TauI GCSGC 2 cut(s) 314, 1121
TfiI GAWTC 8 cut(s) 89, 95, 500, 567, 809, 846, 1416, 1596
Tru1I TTAA 7 cut(s) 822, 1130, 1431, 1512, 1629, 1869, 1908
Tru9I TTAA 7 cut(s) 822, 1130, 1431, 1512, 1629, 1869, 1908
TscAI CASTG 5 cut(s) 127, 226, 448, 964, 1844
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 4 cut(s) 446, 731, 988, 1067
Tsp45I GTSAC 1 cut(s) 616
TspRI CASTG 5 cut(s) 127, 226, 448, 964, 1844
Van91I CCANNNNNTGG 2 cut(s) 511, 1624
VpaK11BI GGWCC 2 cut(s) 1072, 1310
XapI RAATTY 2 cut(s) 8, 1340
XceI RCATGY 4 cut(s) 286, 1161, 1797, 2003
XmnI GAANNNNTTC 1 cut(s) 1464
XspI CTAG 4 cut(s) 450, 1211, 1397, 1857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.