Rh5AG430900

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000175
Physical Location & Seq
Forward (+)
74079958 .. 74093734
13777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG430900.1

Sequence Viewer

Length: 2181 bp
ATGGAAACATTCGTATTTGATCAGGGCTCAATTTCAAACTACTCTTATCCAGATCATGGTTATGGGTTTGAAGTAAATCATGAACTCATCAACCCACTTTTCCTTCCTACCAACTTGGACCTTCTTAGTGATTCTAGTACATCTTTGAAGCCGACTTCAGACAGAAATGGTGGTGACAGTAGTGACTACAATAATCCTGTTCTCAAGTATATAAGTGATATTCTTCTCGAAGAGGACATTGAGGACAAGCCTTGTATGTTACAGGACTGTTTGGCCCTCCAAGCTGCTGAGAAATCTTTCTATGATGTCCTTAATCCAAAGGATGCTACAGTACTCAACCAAAGCTTTGAGAACTCGGATGATTACAGGGAGAGTTGTGATAGCAATGGTGGCTCTATTTTTGGTAAGATTGCTGATCCTTCAGAATCTTGTCATTTCCAGTCTTCTCTTGTTGAGTGTCTATCGGATACCTCCTTGGTTTCTGATTCAAGTTCTAAGCTGCAAAGTCTTGGGAATTTTGGAAGGGTGGAGGAAGCAAACAAGTTCCATGAAAATTTTGAAATCATTGATGCGGAGAGGTACCAATTAATGCTTCAAGGTCCTCAGACACTAATAGGATCAAATTTGGTGTCTGTGCAACAGAGTGATCGGTACAACTCAGCAAATAGATCAGAGGCAAAAAAAAATCGTCAAAGGCAGGATGCTCATTGTCTGGAAGAATGGAGAAGCAACAAGCAGTCAGTTGCATATTTTACTGACGACTCTGAGCCGCAAGAAATGTTTGATAAGGCATTACTCTTTCAAGGTGTGAATCACGAAGCCGAGTGTAGTTCTCATCATGATTCTTTTAATGAGGGAAGTGGGCAGTGCAAAGTATCTAAGCCAGCACGTTCAAAGAGACAAAATAAAAAGGAGAAAGTAGTAGACTTGTGCACAATGTTAACTCAATGCGCGCAAGCTGTTGCAAGTTATGACCAACGAAATGCTAGTGAATTACTGAAGCAGATTAGACAGCACTCTTCTCCCTATGGTGATGCAACCCAGAGATTAGCTCATTACTTTGCTGATGGCCTCGAGGTACGCTTAGCTGGCACTAGAACCCCGTCGTATTCGCCTATTGCAAGCATGCAGATATCAACTGTTGAAATATTGAAAAGTTACCAGACGTTTGTCACTTCAACCCCTTTGAAGACCGTGCCACACTTCTTTGCCAATATGACAATTATGAAACTAGCAGAAAAAGCAACAAGTCTTCACATTATTGATTTTGGTACTTCTTATGGTTTCCAATGGCCCTCTCTCATCCAATGTCTTTCAGAGAGACATGGTGGACCTCCTAAGCTTCGTATCACTACAATTGAGCTTCCCCAACCAGGATTTCGACCCACAGAGAGGGTTGAAGAAACTGGTCGTCGCTTAGCAAAGTATTCTGCAAGATTTAGTGTCCCATTTGAGTTCCATGTCATTGCTCAGAAATGGGAAACTGTTGGATTTGATGATCTTAAGATCGACAGAAATGAGCTGATTGTGGTCAACTGTCTACACCGGTTAAAGCACGTTCCTGATGTGACAATGATGGGGAACAGTCCGAGAGACGCTGTCCTAAAGTTGATCAAGAAAATCAATCCAGAAATTTTCATTCATGGGGTTGTCAATGGGGCATACAACTCACCCTTCTTTCTCACACGTTTCAAACAGGCACTCTTCCACTACGATGCACTCTTTAATGTCTTTGAGGCCACCATTCCTTGTGAGGATCAACAACGACTGTTTTTCGAAAGAGCAGGATATGGTAGAGACATAATGAATGTAGTGGCGTGTGAGGGACTGGAAATAGTTGAAAGGCCTGAGACATATAAGAAGTGGCAGGTCAGGAATGTGAGGGCTGGGTTCAAGCAGCTCCCATTAGACCAGGAGCTGTTGAAGAAGGTGAAGACTATGTCGAAGTCGATGGGTTATCACAATGATTTTAGTATTGATGAAGACGAGCAGTGGATGCTGCAGGGATGGAAAGGAAGAGTCCTCATGGCTCTTTCCTTCTGGAAACGCGATAAGAGGACCAAGAGGGAGGTCATCAGGCTCAAAGTAGAGGGTCCGAAGATCAAAATTAGAGCTCTGGTCGACGGTGAAGATGAAATCTGCTTTGGTCGAGAGCAGCGAGGGCGCTCATGTGAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

726

Amino Acids

82.79

Weight (kDa)

5.95

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 312 - 682 7.3e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1073
Acc36I ACCTGC 1 cut(s) 1858
Acc65I GGTACC 1 cut(s) 579
AccB1I GGYRCC 1 cut(s) 579
AccB7I CCANNNNNTGG 1 cut(s) 56
AccI GTMKAC 3 cut(s) 924, 1540, 2121
AccII CGCG 2 cut(s) 953, 2049
AciI CCGC 2 cut(s) 572, 770
AclWI GGATC 3 cut(s) 410, 625, 1764
AcsI RAATTY 4 cut(s) 514, 553, 622, 1632
AcuI CTGAAG 3 cut(s) 141, 405, 1019
AfaI GTAC 6 cut(s) 139, 333, 581, 653, 1080, 1273
AfiI CCNNNNNNNGG 3 cut(s) 56, 1373, 1392
AflII CTTAAG 1 cut(s) 1502
AflIII ACRYGT 1 cut(s) 1685
AgeI ACCGGT 1 cut(s) 1545
AjnI CCWGG 2 cut(s) 1372, 1911
AjuI GAANNNNNNNTTGG 6 cut(s) 576, 608, 1362, 1394, 2127, 2159
Alw21I GWGCWC 2 cut(s) 935, 2116
Alw26I GTCTC 5 cut(s) 892, 1315, 1587, 1791, 1844
Alw44I GTGCAC 1 cut(s) 931
AlwI GGATC 3 cut(s) 410, 625, 1764
AlwNI CAGNNNCTG 1 cut(s) 1918
Ama87I CYCGRG 1 cut(s) 1073
AoxI GGCC 5 cut(s) 273, 1069, 1292, 1737, 1844
ApaLI GTGCAC 1 cut(s) 931
ApeKI GCWGC 5 cut(s) 284, 499, 1897, 1999, 2155
ApoI RAATTY 4 cut(s) 514, 553, 622, 1632
ArsI GACNNNNNNTTYG 2 cut(s) 614, 646
AseI ATTAAT 1 cut(s) 587
AsiGI ACCGGT 1 cut(s) 1545
Asp700I GAANNNNTTC 1 cut(s) 296
Asp718I GGTACC 1 cut(s) 579
AspLEI GCGC 3 cut(s) 953, 955, 2166
AspS9I GGNCC 7 cut(s) 118, 274, 599, 1293, 1331, 2058, 2093
AsuHPI GGTGA 5 cut(s) 185, 1043, 1662, 1942, 2138
AsuII TTCGAA 1 cut(s) 1776
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 5 cut(s) 118, 599, 1331, 2058, 2093
BaeGI GKGCMC 1 cut(s) 935
BanI GGYRCC 1 cut(s) 579
BanII GRGCYC 2 cut(s) 29, 2116
BbsI GAAGAC 5 cut(s) 435, 1196, 1244, 1940, 1989
Bbv12I GWGCWC 2 cut(s) 935, 2116
BbvI GCAGC 5 cut(s) 271, 486, 1909, 1986, 2167
BccI CCATC 4 cut(s) 1061, 1570, 1945, 2001
BciT130I CCWGG 2 cut(s) 1374, 1913
BciVI GTATCC 1 cut(s) 460
BclI TGATCA 2 cut(s) 19, 1611
BcoDI GTCTC 5 cut(s) 892, 1315, 1587, 1791, 1844
BfaI CTAG 4 cut(s) 135, 987, 1095, 1232
BfmI CTRYAG 2 cut(s) 327, 2000
BfoI RGCGCY 1 cut(s) 2167
BfrI CTTAAG 1 cut(s) 1502
BfuAI ACCTGC 1 cut(s) 1858
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 6 cut(s) 285, 500, 770, 1898, 2000, 2156
BlpI GCTNAGC 2 cut(s) 1084, 1417
BlsI GCNGC 6 cut(s) 286, 501, 771, 1899, 2001, 2157
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 2 cut(s) 1374, 1913
Bme18I GGWCC 5 cut(s) 118, 599, 1331, 2058, 2093
BmeT110I CYCGRG 1 cut(s) 1073
BmgT120I GGNCC 7 cut(s) 118, 274, 599, 1293, 1331, 2058, 2093
BmiI GGNNCC 2 cut(s) 581, 2094
BmrFI CCNGG 2 cut(s) 1374, 1913
BmsI GCATC 6 cut(s) 313, 559, 691, 1024, 1705, 1986
BoxI GACNNNNGTC 1 cut(s) 1169
BpiI GAAGAC 5 cut(s) 435, 1196, 1244, 1940, 1989
BplI GAGNNNNNCTC 2 cut(s) 1036, 1068
Bpu10I CCTNAGC 1 cut(s) 1338
Bpu1102I GCTNAGC 2 cut(s) 1084, 1417
Bpu14I TTCGAA 1 cut(s) 1776
BpuEI CTTGAG 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 1545
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 1373, 1392
Bse118I RCCGGY 1 cut(s) 1545
Bse1I ACTGG 3 cut(s) 439, 1411, 1833
Bse3DI GCAATG 2 cut(s) 391, 1464
BseBI CCWGG 2 cut(s) 1374, 1913
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 6 cut(s) 328, 364, 706, 1302, 2001, 2012
BseLI CCNNNNNNNGG 3 cut(s) 56, 1373, 1392
BseMI GCAATG 2 cut(s) 391, 1464
BseMII CTCAG 6 cut(s) 279, 617, 672, 756, 1484, 1839
BseNI ACTGG 3 cut(s) 439, 1411, 1833
BsePI GCGCGC 1 cut(s) 951
BseSI GKGCMC 1 cut(s) 935
BseXI GCAGC 5 cut(s) 271, 486, 1909, 1986, 2167
BseYI CCCAGC 1 cut(s) 1886
Bsh1236I CGCG 2 cut(s) 953, 2049
BshFI GGCC 5 cut(s) 275, 1071, 1294, 1739, 1846
BshNI GGYRCC 1 cut(s) 579
BshTI ACCGGT 1 cut(s) 1545
BsiHKAI GWGCWC 2 cut(s) 935, 2116
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 1 cut(s) 1546
BslFI GGGAC 2 cut(s) 1430, 1839
BslI CCNNNNNNNGG 3 cut(s) 56, 1373, 1392
BsmAI GTCTC 5 cut(s) 892, 1315, 1587, 1791, 1844
BsmBI CGTCTC 1 cut(s) 1587
BsmFI GGGAC 2 cut(s) 1430, 1839
BsnI GGCC 5 cut(s) 275, 1071, 1294, 1739, 1846
BsoBI CYCGRG 1 cut(s) 1073
Bsp119I TTCGAA 1 cut(s) 1776
Bsp1286I GDGCHC 3 cut(s) 29, 935, 2116
Bsp1720I GCTNAGC 2 cut(s) 1084, 1417
BspACI CCGC 2 cut(s) 572, 770
BspANI GGCC 5 cut(s) 275, 1071, 1294, 1739, 1846
BspCNI CTCAG 6 cut(s) 280, 616, 671, 757, 1483, 1840
BspFNI CGCG 2 cut(s) 953, 2049
BspHI TCATGA 2 cut(s) 79, 838
BspLI GGNNCC 2 cut(s) 581, 2094
BspMAI CTGCAG 1 cut(s) 2004
BspMI ACCTGC 1 cut(s) 1858
BspPI GGATC 3 cut(s) 410, 625, 1764
BspT104I TTCGAA 1 cut(s) 1776
BspT107I GGYRCC 1 cut(s) 579
BspTI CTTAAG 1 cut(s) 1502
BsrDI GCAATG 2 cut(s) 391, 1464
BsrFI RCCGGY 1 cut(s) 1545
BsrI ACTGG 3 cut(s) 439, 1411, 1833
BssAI RCCGGY 1 cut(s) 1545
BssECI CCNNGG 1 cut(s) 474
BssHII GCGCGC 1 cut(s) 951
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 1374, 1913
Bst6I CTCTTC 4 cut(s) 225, 1024, 1709, 2011
BstAFI CTTAAG 1 cut(s) 1502
BstAPI GCANNNNNTGC 1 cut(s) 1996
BstBI TTCGAA 1 cut(s) 1776
BstC8I GCNNGC 6 cut(s) 885, 953, 957, 1090, 1123, 1127
BstF5I GGATG 6 cut(s) 328, 364, 706, 1302, 2001, 2012
BstFNI CGCG 2 cut(s) 953, 2049
BstH2I RGCGCY 1 cut(s) 2167
BstHHI GCGC 3 cut(s) 953, 955, 2166
BstMAI GTCTC 5 cut(s) 892, 1315, 1587, 1791, 1844
BstMWI GCNNNNNNNGC 6 cut(s) 281, 390, 1089, 1241, 1996, 2161
BstNI CCWGG 2 cut(s) 1374, 1913
BstNSI RCATGY 1 cut(s) 1129
BstPAI GACNNNNGTC 1 cut(s) 1169
BstSCI CCNGG 2 cut(s) 1372, 1911
BstSFI CTRYAG 2 cut(s) 327, 2000
BstSLI GKGCMC 1 cut(s) 935
BstUI CGCG 2 cut(s) 953, 2049
BstV1I GCAGC 5 cut(s) 271, 486, 1909, 1986, 2167
BstV2I GAAGAC 5 cut(s) 435, 1196, 1244, 1940, 1989
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 5 cut(s) 275, 1071, 1294, 1739, 1846
BtsCI GGATG 6 cut(s) 328, 364, 706, 1302, 2001, 2012
BtsI GCAGTG 2 cut(s) 872, 1997
BtsIMutI CAGTG 2 cut(s) 872, 1997
BveI ACCTGC 1 cut(s) 1858
Cac8I GCNNGC 6 cut(s) 885, 953, 957, 1090, 1123, 1127
CaiI CAGNNNCTG 1 cut(s) 1918
CciI TCATGA 2 cut(s) 79, 838
CfoI GCGC 3 cut(s) 953, 955, 2166
Cfr10I RCCGGY 1 cut(s) 1545
Cfr13I GGNCC 7 cut(s) 118, 274, 599, 1293, 1331, 2058, 2093
CseI GACGC 1 cut(s) 1604
Csp6I GTAC 6 cut(s) 138, 332, 580, 652, 1079, 1272
CspAI ACCGGT 1 cut(s) 1545
CviQI GTAC 6 cut(s) 138, 332, 580, 652, 1079, 1272
Eam1104I CTCTTC 4 cut(s) 225, 1024, 1709, 2011
EarI CTCTTC 4 cut(s) 225, 1024, 1709, 2011
Ecl136II GAGCTC 1 cut(s) 2114
Eco130I CCWWGG 1 cut(s) 474
Eco147I AGGCCT 1 cut(s) 1846
Eco24I GRGCYC 2 cut(s) 29, 2116
Eco32I GATATC 1 cut(s) 1134
Eco47I GGWCC 5 cut(s) 118, 599, 1331, 2058, 2093
Eco53kI GAGCTC 1 cut(s) 2114
Eco57I CTGAAG 3 cut(s) 141, 405, 1019
Eco88I CYCGRG 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 2114
EcoO109I RGGNCCY 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 1372, 1911
EcoRV GATATC 1 cut(s) 1134
EcoT14I CCWWGG 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 29, 2116
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 1587
FaqI GGGAC 2 cut(s) 1430, 1839
FbaI TGATCA 2 cut(s) 19, 1611
FblI GTMKAC 3 cut(s) 924, 1540, 2121
Fnu4HI GCNGC 6 cut(s) 285, 500, 770, 1898, 2000, 2156
FokI GGATG 6 cut(s) 335, 371, 713, 1289, 2008, 2019
FriOI GRGCYC 2 cut(s) 29, 2116
Fsp4HI GCNGC 6 cut(s) 285, 500, 770, 1898, 2000, 2156
FspBI CTAG 4 cut(s) 135, 987, 1095, 1232
GlaI GCGC 3 cut(s) 952, 954, 2165
GluI GCNGC 6 cut(s) 285, 500, 770, 1898, 2000, 2156
GsaI CCCAGC 1 cut(s) 1890
HaeII RGCGCY 1 cut(s) 2167
HaeIII GGCC 5 cut(s) 275, 1071, 1294, 1739, 1846
HapII CCGG 1 cut(s) 1546
HgaI GACGC 1 cut(s) 1604
HhaI GCGC 3 cut(s) 953, 955, 2166
Hin6I GCGC 3 cut(s) 951, 953, 2164
HinP1I GCGC 3 cut(s) 951, 953, 2164
HincII GTYRAC 3 cut(s) 942, 1534, 2122
HindII GTYRAC 3 cut(s) 942, 1534, 2122
HindIII AAGCTT 2 cut(s) 343, 1340
HinfI GANTC 7 cut(s) 131, 425, 485, 761, 811, 842, 2019
HpaI GTTAAC 1 cut(s) 942
HpaII CCGG 1 cut(s) 1546
HphI GGTGA 5 cut(s) 185, 1043, 1662, 1942, 2138
Hpy166II GTNNAC 7 cut(s) 925, 933, 942, 1331, 1534, 1541, 2122
Hpy8I GTNNAC 7 cut(s) 925, 933, 942, 1331, 1534, 1541, 2122
Hpy99I CGWCG 3 cut(s) 1108, 1416, 2126
HpyAV CCTTC 7 cut(s) 113, 131, 429, 516, 1684, 1921, 2047
HpyCH4IV ACGT 4 cut(s) 889, 1166, 1557, 1687
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 390, 1089, 1241, 1996, 2161
HpySE526I ACGT 4 cut(s) 889, 1166, 1557, 1687
HspAI GCGC 3 cut(s) 951, 953, 2164
KpnI GGTACC 1 cut(s) 583
Ksp22I TGATCA 2 cut(s) 19, 1611
KspAI GTTAAC 1 cut(s) 942
LmnI GCTCC 2 cut(s) 1905, 1915
Lsp1109I GCAGC 5 cut(s) 271, 486, 1909, 1986, 2167
LweI GCATC 6 cut(s) 313, 559, 691, 1024, 1705, 1986
MaeI CTAG 4 cut(s) 135, 987, 1095, 1232
MaeII ACGT 4 cut(s) 889, 1166, 1557, 1687
MaeIII GTNAC 6 cut(s) 173, 182, 258, 1157, 1171, 1567
MfeI CAATTG 1 cut(s) 1356
MhlI GDGCHC 3 cut(s) 29, 935, 2116
MlyI GAGTC 2 cut(s) 755, 2028
MmeI TCCRAC 1 cut(s) 1468
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 7 cut(s) 312, 587, 849, 941, 1503, 1550, 1725
MslI CAYNNNNRTG 2 cut(s) 60, 1713
MspCI CTTAAG 1 cut(s) 1502
MspI CCGG 1 cut(s) 1546
MspR9I CCNGG 2 cut(s) 1374, 1913
MunI CAATTG 1 cut(s) 1356
MvaI CCWGG 2 cut(s) 1374, 1913
MvnI CGCG 2 cut(s) 953, 2049
MwoI GCNNNNNNNGC 6 cut(s) 281, 390, 1089, 1241, 1996, 2161
NlaIV GGNNCC 2 cut(s) 581, 2094
NmeAIII GCCGAG 1 cut(s) 847
NmuCI GTSAC 4 cut(s) 173, 182, 1171, 1567
NspI RCATGY 1 cut(s) 1129
NspV TTCGAA 1 cut(s) 1776
PaeI GCATGC 1 cut(s) 1129
PaeR7I CTCGAG 1 cut(s) 1073
PagI TCATGA 2 cut(s) 79, 838
PauI GCGCGC 1 cut(s) 951
PceI AGGCCT 1 cut(s) 1846
PcsI WCGNNNNNNNCGW 1 cut(s) 2155
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 5 cut(s) 131, 425, 485, 811, 842
PflFI GACNNNGTC 2 cut(s) 1598, 1939
PflMI CCANNNNNTGG 1 cut(s) 56
PinAI ACCGGT 1 cut(s) 1545
PkrI GCNGC 6 cut(s) 286, 501, 771, 1899, 2001, 2157
PleI GAGTC 2 cut(s) 755, 2027
PpsI GAGTC 2 cut(s) 755, 2027
PpuMI RGGWCCY 1 cut(s) 599
PshAI GACNNNNGTC 1 cut(s) 1169
PshBI ATTAAT 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 2116
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 1372, 1911
PspFI CCCAGC 1 cut(s) 1886
PspGI CCWGG 2 cut(s) 1372, 1911
PspN4I GGNNCC 2 cut(s) 581, 2094
PspPI GGNCC 7 cut(s) 118, 274, 599, 1293, 1331, 2058, 2093
PspPPI RGGWCCY 1 cut(s) 599
PspXI VCTCGAGB 1 cut(s) 1073
PstI CTGCAG 1 cut(s) 2004
PstNI CAGNNNCTG 1 cut(s) 1918
PsyI GACNNNGTC 2 cut(s) 1598, 1939
PteI GCGCGC 1 cut(s) 951
RsaI GTAC 6 cut(s) 139, 333, 581, 653, 1080, 1273
RsaNI GTAC 6 cut(s) 138, 332, 580, 652, 1079, 1272
RseI CAYNNNNRTG 2 cut(s) 60, 1713
SacI GAGCTC 1 cut(s) 2116
SalI GTCGAC 1 cut(s) 2120
SaqAI TTAA 7 cut(s) 312, 587, 849, 941, 1503, 1550, 1725
SatI GCNGC 6 cut(s) 285, 500, 770, 1898, 2000, 2156
Sau96I GGNCC 7 cut(s) 118, 274, 599, 1293, 1331, 2058, 2093
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 2 cut(s) 755, 2028
ScrFI CCNGG 2 cut(s) 1374, 1913
SduI GDGCHC 3 cut(s) 29, 935, 2116
SfaNI GCATC 6 cut(s) 313, 559, 691, 1024, 1705, 1986
SfcI CTRYAG 2 cut(s) 327, 2000
Sfr274I CTCGAG 1 cut(s) 1073
SfuI TTCGAA 1 cut(s) 1776
SinI GGWCC 5 cut(s) 118, 599, 1331, 2058, 2093
SlaI CTCGAG 1 cut(s) 1073
SmiMI CAYNNNNRTG 2 cut(s) 60, 1713
SmlI CTYRAG 3 cut(s) 203, 1073, 1502
SmoI CTYRAG 3 cut(s) 203, 1073, 1502
SphI GCATGC 1 cut(s) 1129
SseBI AGGCCT 1 cut(s) 1846
SsiI CCGC 2 cut(s) 572, 770
SspI AATATT 1 cut(s) 1149
SspMI CTAG 4 cut(s) 135, 987, 1095, 1232
SstI GAGCTC 1 cut(s) 2116
StuI AGGCCT 1 cut(s) 1846
StyD4I CCNGG 2 cut(s) 1372, 1911
StyI CCWWGG 1 cut(s) 474
TaiI ACGT 4 cut(s) 892, 1169, 1560, 1690
TaqI TCGA 9 cut(s) 228, 1074, 1381, 1509, 1776, 1943, 1949, 2121, 2149
TatI WGTACW 2 cut(s) 137, 331
TauI GCSGC 1 cut(s) 772
TfiI GAWTC 5 cut(s) 131, 425, 485, 811, 842
Tru1I TTAA 7 cut(s) 312, 587, 849, 941, 1503, 1550, 1725
Tru9I TTAA 7 cut(s) 312, 587, 849, 941, 1503, 1550, 1725
TscAI CASTG 2 cut(s) 872, 1997
TseFI GTSAC 4 cut(s) 173, 182, 1171, 1567
TseI GCWGC 5 cut(s) 284, 499, 1897, 1999, 2155
Tsp45I GTSAC 4 cut(s) 173, 182, 1171, 1567
TspDTI ATGAA 8 cut(s) 96, 564, 1241, 1627, 1631, 1820, 1995, 2148
TspRI CASTG 2 cut(s) 872, 1997
Tth111I GACNNNGTC 2 cut(s) 1598, 1939
Van91I CCANNNNNTGG 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 1502
VneI GTGCAC 1 cut(s) 931
VpaK11BI GGWCC 5 cut(s) 118, 599, 1331, 2058, 2093
VspI ATTAAT 1 cut(s) 587
XapI RAATTY 4 cut(s) 514, 553, 622, 1632
XceI RCATGY 1 cut(s) 1129
XhoI CTCGAG 1 cut(s) 1073
XmiI GTMKAC 3 cut(s) 924, 1540, 2121
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 4 cut(s) 135, 987, 1095, 1232
ZrmI AGTACT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.