Rh5DG461900

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000175
Physical Location & Seq
Reverse (-)
73287888 .. 73291438
3551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG461900.1

Sequence Viewer

Length: 1563 bp
ATGCCAACATCTCCAGATCAGAATATCATTTTTACTGAGTTTGATCAACACAATCTTGAAATTACTGATCCCAGTTCGTTTCTATCCAATCCAAACCCTCCTCCTCGTGATGTTTCATCCCGGTCTTTGGGTGTGACCTCAGAGGCCTCCAGAGCTACTCCCCAGTATATAAGTGAGATGCTTATGGAGGAAGAGCTGGAGAACAGACCCTGCATGCTTCAGGACTGTTTGGCCCTCCAAGCTGCTGAGAAATCCTTGGAAGATGTCCTGGTTCAGCAATATCCTCCTTCGACTAATCCCATTCTTGCTTCTTCCATTCAGCAAAATGTTGACAACTCTGATGATTTCATTAACCACAGCAGTAATAGCTCCACTGCTGCTAGAAACTGGGTTGATGGTTCAGGTTGGATTTCCTTTCAAAATGATCACAATGACTCAACAAAAGGATCAAGGAGCAAAAAAGATCGTCATTGGGAGGATGGCGATTGTCTAGAAGAAGGGAGGAGCAATAAGCAGTCATCATTTTACGCTGATGAATCAGAGCCACCAGATATGTTGGATAAGGTACTGCTCTTTCATTATCAAAACCCAAAGTCTGATTCATGTTCTTCTTTTCAGCTTAAAGGGGGAAGGGGACATGCAGGATCCAAAGGCAAAAGAACAGTTAAGAAAAAAAAGGATGACAACACTGAAGTAGTGGATTTTCAGTCACTGCTAACTCAGTGTGCAAAGGCAAGCTATGACAGAAGGACTGCAAATGAACAACTGAAACAGATAAGGCAGCACTCATCTCCCTACGGTGATGGAACCCAAAGAGTAGCTCATTACTTAGCCAATGGCCTTGAAGAACACTTGGCTGCTGCAGTTCCTTCGTTCAATCCTCTTTGCCTTAATAAGATGTCAGCTGCTGATATCTTAAAAGCTTACCAGACATATATCAAAGCATGCCCCTTCAAGTTGATGTCAAACATCTATGCTAACAAAACTATCTTCAAACTAACAGAGAAAGCAACAAGGCTTCACATAATTGATTTTGGTATTCTCTATGGCTACCAATGGCCTGGCCTTATCCAAAGTCTTGCTAAAAGACCCTCTGGACCTCCCATGCTTCGCATTACTGGTATTGAATTTCCACAATCAGGATTTCGACCTTCAGAGAGTCTTGAAGAGACAGGGCACCTCCTAGCGAAATATTGCAAGAGATTCAATGTCCCATTTGAGTACAACTTCATAGCACAGGATTGGGAAACCATTCGATATCAAGAGATCAAACTTGACAGAGATGAGTTTACTGTAGTGAACTGCTTGTGCAGATTAAGGAACGTACCTGAAGAAACAGAGATGAGGAGTCCAAGGGATAGAGTTTTGAAGCTGATCAGGAGAATCAACCCAGATATGTATATCCTTGGACTGGTTAATGGAACCTATAATGCACCCTTCTTCAACATACGGTTCCGGGAGGCACTCTACCATTTCTCTTCCCTGTTTGATATGTTTGACGAGACTTTGCCCAGAGATGATCAACAGAGTCTGCTCTACACACAAGAGATATTATCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

520

Amino Acids

59.38

Weight (kDa)

5.73

Isoelectric Point (pI)

64.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 236 - 518 1.3e-81 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1176
AclWI GGATC 4 cut(s) 62, 454, 639, 652
AcsI RAATTY 1 cut(s) 1127
AcuI CTGAAG 4 cut(s) 203, 711, 1137, 1350
AfaI GTAC 3 cut(s) 567, 1223, 1326
AfiI CCNNNNNNNGG 3 cut(s) 127, 1139, 1411
AjnI CCWGG 2 cut(s) 267, 1060
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
Alw26I GTCTC 2 cut(s) 1163, 1496
AlwI GGATC 4 cut(s) 62, 454, 639, 652
AlwNI CAGNNNCTG 4 cut(s) 210, 712, 908, 1531
AoxI GGCC 5 cut(s) 144, 231, 838, 1058, 1063
ApeKI GCWGC 6 cut(s) 242, 377, 781, 857, 860, 905
ApoI RAATTY 1 cut(s) 1127
Asp700I GAANNNNTTC 1 cut(s) 1251
AspS9I GGNCC 2 cut(s) 232, 1097
AsuC2I CCSGG 2 cut(s) 121, 1457
AsuHPI GGTGA 1 cut(s) 812
AvaII GGWCC 1 cut(s) 1097
BaeGI GKGCMC 1 cut(s) 1179
BamHI GGATCC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 1176
BauI CACGAG 1 cut(s) 105
BbvI GCAGC 6 cut(s) 229, 364, 793, 844, 847, 892
BccI CCATC 3 cut(s) 389, 473, 797
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 2 cut(s) 269, 1062
BclI TGATCA 4 cut(s) 43, 424, 1374, 1519
BcnI CCSGG 2 cut(s) 121, 1457
BcoDI GTCTC 2 cut(s) 1163, 1496
BfaI CTAG 3 cut(s) 381, 491, 1184
BfmI CTRYAG 2 cut(s) 861, 1293
BisI GCNGC 6 cut(s) 243, 378, 782, 858, 861, 906
BlsI GCNGC 6 cut(s) 244, 379, 783, 859, 862, 907
Bme1390I CCNGG 4 cut(s) 121, 269, 1062, 1457
Bme18I GGWCC 1 cut(s) 1097
BmgT120I GGNCC 2 cut(s) 232, 1097
BmiI GGNNCC 5 cut(s) 646, 808, 1178, 1423, 1454
BmrFI CCNGG 4 cut(s) 121, 269, 1062, 1457
BmrI ACTGGG 3 cut(s) 66, 157, 397
BmsI GCATC 1 cut(s) 168
BmuI ACTGGG 3 cut(s) 66, 157, 397
BpmI CTGGAG 2 cut(s) 133, 218
BpuMI CCSGG 2 cut(s) 121, 1457
BsaJI CCNNGG 3 cut(s) 255, 1352, 1405
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 1139, 1411
Bse1I ACTGG 5 cut(s) 72, 163, 392, 1123, 1416
BseBI CCWGG 2 cut(s) 269, 1062
BseDI CCNNGG 3 cut(s) 255, 1352, 1405
BseGI GGATG 3 cut(s) 116, 484, 685
BseLI CCNNNNNNNGG 3 cut(s) 127, 1139, 1411
BseMII CTCAG 4 cut(s) 27, 153, 237, 734
BseNI ACTGG 5 cut(s) 72, 163, 392, 1123, 1416
BseRI GAGGAG 4 cut(s) 90, 93, 517, 1360
BseSI GKGCMC 1 cut(s) 1179
BseXI GCAGC 6 cut(s) 229, 364, 793, 844, 847, 892
BsgI GTGCAG 1 cut(s) 1330
BshFI GGCC 5 cut(s) 146, 233, 840, 1060, 1065
BshNI GGYRCC 1 cut(s) 1176
BsiSI CCGG 2 cut(s) 121, 1456
BslFI GGGAC 2 cut(s) 648, 1196
BslI CCNNNNNNNGG 3 cut(s) 127, 1139, 1411
BsmAI GTCTC 2 cut(s) 1163, 1496
BsmFI GGGAC 2 cut(s) 648, 1196
BsnI GGCC 5 cut(s) 146, 233, 840, 1060, 1065
Bsp1286I GDGCHC 1 cut(s) 1179
BspANI GGCC 5 cut(s) 146, 233, 840, 1060, 1065
BspCNI CTCAG 4 cut(s) 28, 152, 238, 733
BspLI GGNNCC 5 cut(s) 646, 808, 1178, 1423, 1454
BspMAI CTGCAG 1 cut(s) 865
BspPI GGATC 4 cut(s) 62, 454, 639, 652
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 1176
BsrI ACTGG 5 cut(s) 72, 163, 392, 1123, 1416
BssECI CCNNGG 3 cut(s) 255, 1352, 1405
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 3 cut(s) 255, 1352, 1405
Bst2BI CACGAG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 269, 1062
Bst4CI ACNGT 5 cut(s) 227, 664, 800, 1294, 1452
Bst6I CTCTTC 3 cut(s) 186, 1161, 1483
BstC8I GCNNGC 3 cut(s) 215, 736, 946
BstDEI CTNAG 5 cut(s) 36, 139, 246, 720, 829
BstF5I GGATG 3 cut(s) 116, 484, 685
BstMAI GTCTC 2 cut(s) 1163, 1496
BstMWI GCNNNNNNNGC 3 cut(s) 152, 239, 366
BstNI CCWGG 2 cut(s) 269, 1062
BstNSI RCATGY 3 cut(s) 217, 641, 948
BstSCI CCNGG 4 cut(s) 119, 267, 1060, 1455
BstSFI CTRYAG 2 cut(s) 861, 1293
BstSLI GKGCMC 1 cut(s) 1179
BstV1I GCAGC 6 cut(s) 229, 364, 793, 844, 847, 892
BstX2I RGATCY 1 cut(s) 644
BstXI CCANNNNNNTGG 1 cut(s) 1061
BstYI RGATCY 1 cut(s) 644
BsuRI GGCC 5 cut(s) 146, 233, 840, 1060, 1065
BtsCI GGATG 3 cut(s) 116, 484, 685
BtsI GCAGTG 2 cut(s) 372, 710
BtsIMutI CAGTG 4 cut(s) 372, 687, 710, 728
Cac8I GCNNGC 3 cut(s) 215, 736, 946
CaiI CAGNNNCTG 4 cut(s) 210, 712, 908, 1531
Cfr13I GGNCC 2 cut(s) 232, 1097
Csp6I GTAC 3 cut(s) 566, 1222, 1325
CviAII CATG 5 cut(s) 214, 603, 638, 945, 1105
CviQI GTAC 3 cut(s) 566, 1222, 1325
DdeI CTNAG 5 cut(s) 36, 139, 246, 720, 829
Eam1104I CTCTTC 3 cut(s) 186, 1161, 1483
EarI CTCTTC 3 cut(s) 186, 1161, 1483
Eco130I CCWWGG 3 cut(s) 255, 1352, 1405
Eco147I AGGCCT 1 cut(s) 146
Eco32I GATATC 2 cut(s) 913, 1259
Eco47I GGWCC 1 cut(s) 1097
Eco57I CTGAAG 4 cut(s) 203, 711, 1137, 1350
EcoRII CCWGG 2 cut(s) 267, 1060
EcoRV GATATC 2 cut(s) 913, 1259
EcoT14I CCWWGG 3 cut(s) 255, 1352, 1405
ErhI CCWWGG 3 cut(s) 255, 1352, 1405
FaeI CATG 5 cut(s) 217, 606, 641, 948, 1108
FaqI GGGAC 2 cut(s) 648, 1196
FatI CATG 5 cut(s) 213, 602, 637, 944, 1104
FbaI TGATCA 4 cut(s) 43, 424, 1374, 1519
Fnu4HI GCNGC 6 cut(s) 243, 378, 782, 858, 861, 906
FokI GGATG 3 cut(s) 103, 491, 692
Fsp4HI GCNGC 6 cut(s) 243, 378, 782, 858, 861, 906
FspBI CTAG 3 cut(s) 381, 491, 1184
GluI GCNGC 6 cut(s) 243, 378, 782, 858, 861, 906
GsuI CTGGAG 2 cut(s) 133, 218
HaeIII GGCC 5 cut(s) 146, 233, 840, 1060, 1065
HapII CCGG 2 cut(s) 121, 1456
Hin1II CATG 5 cut(s) 217, 606, 641, 948, 1108
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 1 cut(s) 921
HinfI GANTC 8 cut(s) 434, 536, 599, 1159, 1203, 1348, 1383, 1528
HpaII CCGG 2 cut(s) 121, 1456
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 3 cut(s) 331, 1290, 1300
Hpy188I TCNGA 6 cut(s) 21, 142, 340, 541, 598, 1156
Hpy8I GTNNAC 3 cut(s) 331, 1290, 1300
HpyAV CCTTC 8 cut(s) 297, 491, 624, 741, 879, 961, 1161, 1447
HpyCH4III ACNGT 5 cut(s) 227, 664, 800, 1294, 1452
HpyCH4IV ACGT 1 cut(s) 1323
HpyCH4V TGCA 8 cut(s) 213, 641, 728, 755, 863, 1197, 1311, 1433
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 239, 366
HpyF3I CTNAG 5 cut(s) 36, 139, 246, 720, 829
HpySE526I ACGT 1 cut(s) 1323
Hsp92II CATG 5 cut(s) 217, 606, 641, 948, 1108
Ksp22I TGATCA 4 cut(s) 43, 424, 1374, 1519
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 374, 453, 504
Lsp1109I GCAGC 6 cut(s) 229, 364, 793, 844, 847, 892
LweI GCATC 1 cut(s) 168
MaeI CTAG 3 cut(s) 381, 491, 1184
MaeII ACGT 1 cut(s) 1323
MaeIII GTNAC 2 cut(s) 133, 708
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 1 cut(s) 1179
MluCI AATT 3 cut(s) 60, 1026, 1127
MlyI GAGTC 4 cut(s) 428, 1168, 1357, 1537
MmeI TCCRAC 2 cut(s) 386, 537
MroXI GAANNNNTTC 1 cut(s) 1251
MseI TTAA 7 cut(s) 351, 621, 666, 891, 917, 1316, 1416
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 2 cut(s) 121, 1456
MspR9I CCNGG 4 cut(s) 121, 269, 1062, 1457
MvaI CCWGG 2 cut(s) 269, 1062
MwoI GCNNNNNNNGC 3 cut(s) 152, 239, 366
NciI CCSGG 2 cut(s) 121, 1457
NlaIII CATG 5 cut(s) 217, 606, 641, 948, 1108
NlaIV GGNNCC 5 cut(s) 646, 808, 1178, 1423, 1454
NmuCI GTSAC 2 cut(s) 133, 708
NspI RCATGY 3 cut(s) 217, 641, 948
PaeI GCATGC 2 cut(s) 217, 948
PceI AGGCCT 1 cut(s) 146
PciSI GCTCTTC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 1251
PfeI GAWTC 4 cut(s) 536, 599, 1203, 1383
PfoI TCCNGGA 1 cut(s) 1455
PkrI GCNGC 6 cut(s) 244, 379, 783, 859, 862, 907
PleI GAGTC 4 cut(s) 428, 1167, 1356, 1536
PpsI GAGTC 4 cut(s) 428, 1167, 1356, 1536
Psp6I CCWGG 2 cut(s) 267, 1060
PspGI CCWGG 2 cut(s) 267, 1060
PspN4I GGNNCC 5 cut(s) 646, 808, 1178, 1423, 1454
PspPI GGNCC 2 cut(s) 232, 1097
PstI CTGCAG 1 cut(s) 865
PstNI CAGNNNCTG 4 cut(s) 210, 712, 908, 1531
PsuI RGATCY 1 cut(s) 644
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 3 cut(s) 567, 1223, 1326
RsaNI GTAC 3 cut(s) 566, 1222, 1325
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 7 cut(s) 351, 621, 666, 891, 917, 1316, 1416
SatI GCNGC 6 cut(s) 243, 378, 782, 858, 861, 906
Sau96I GGNCC 2 cut(s) 232, 1097
SchI GAGTC 4 cut(s) 428, 1168, 1357, 1537
ScrFI CCNGG 4 cut(s) 121, 269, 1062, 1457
SduI GDGCHC 1 cut(s) 1179
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 2 cut(s) 861, 1293
SinI GGWCC 1 cut(s) 1097
SphI GCATGC 2 cut(s) 217, 948
Sse9I AATT 3 cut(s) 60, 1026, 1127
SseBI AGGCCT 1 cut(s) 146
SspI AATATT 1 cut(s) 1193
SspMI CTAG 3 cut(s) 381, 491, 1184
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 4 cut(s) 119, 267, 1060, 1455
StyI CCWWGG 3 cut(s) 255, 1352, 1405
TaaI ACNGT 5 cut(s) 227, 664, 800, 1294, 1452
TaiI ACGT 1 cut(s) 1326
TaqI TCGA 3 cut(s) 290, 1147, 1255
TasI AATT 3 cut(s) 60, 1026, 1127
TatI WGTACW 1 cut(s) 1221
TfiI GAWTC 4 cut(s) 536, 599, 1203, 1383
Tru1I TTAA 7 cut(s) 351, 621, 666, 891, 917, 1316, 1416
Tru9I TTAA 7 cut(s) 351, 621, 666, 891, 917, 1316, 1416
TscAI CASTG 4 cut(s) 379, 694, 717, 728
TseFI GTSAC 2 cut(s) 133, 708
TseI GCWGC 6 cut(s) 242, 377, 781, 857, 860, 905
Tsp45I GTSAC 2 cut(s) 133, 708
TspDTI ATGAA 7 cut(s) 105, 337, 549, 566, 591, 774, 1219
TspRI CASTG 4 cut(s) 379, 694, 717, 728
VpaK11BI GGWCC 1 cut(s) 1097
XapI RAATTY 1 cut(s) 1127
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 3 cut(s) 217, 641, 948
XmnI GAANNNNTTC 1 cut(s) 1251
XspI CTAG 3 cut(s) 381, 491, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.