Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0000175

Physical Location & Seq

Genomic Coordinates Forward (+)

19320103 .. 19320960

Gene Structure 858 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00015560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGATGTCACAATTATTTGCCAACAGAGCAATCATGAAGCTAGCAAAAAATGAAACAAGGCTTCACATTATTGATTTTGGTATTTCTTATGGTTTCCAATGGCCCTTCCTTATCCAACATCTCTCCGAGAGACCGAATGGACCTCCCAAGTTACACATCACAGGAATTGAGCTTCCGCAACGTGGTTTTCGACCTAGAGAAAGGGTTGAAGAGACTGGGCGTCGCCTAGCAAGGTATTGTGCAAAGTTCAATGTCCCGTTTGAGTACGATGTCATTGCTCAAGGATGGGAAACCATACAATGTGAGGATCTTAAAATTGATCAAAACGAACTGGTAGTGGTAAATTGTCTGAACCGGTTAAAGCACATACATGATGAGACGGTGATGGAGAACTCTCCGAGAGATACTGTCCTGAATTTGATCAAGAATATAGATCCCGACCTTTTCATTCATGGGATTGTCAATGGGATGTACAATACACCCTTCTTTGCCACACGGTTCAAAGAGGCACTCTTGCACTACTATGCCGTGTTTGATATGTTTGAAGCAACTGTTCCTCGTGACAATGAACGGCGGATGATTATCGAGAGAGAAACAAGTGGTAGAGACATAATGAATATCGTAGCATGCGAGGGAGTGGAAAGGTTTCAAAGACCTGAGACATATAAGAAATGGCAGATCAGGAATGTGAGGGCTGGTTTGAAGCAGCTCCCGTTGGACCAGGAGGAGTTGAAGAAAGTGAGGAACTTGTATAAGGGTTATCATAATGATTTTAGAGTTGAAGAAGATGGGCAGTGGATACTGCAGGGATGGAAAGGAAGGATCCTCCGGGCTCTTTCACTTTGGACTCCTGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

285

Amino Acids

33.69

Weight (kDa)

8.59

Isoelectric Point (pI)

48.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	2 - 283	2.7e-92	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca	FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica	MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica	Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis	pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis	RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata	RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora	Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii	Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa	Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha	Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana	Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	176, 574
AclWI	GGATC	4 cut(s)	315, 428, 817, 830
AcsI	RAATTY	1 cut(s)	415
AcyI	GRCGYC	1 cut(s)	220
AfaI	GTAC	2 cut(s)	266, 473
AfiI	CCNNNNNNNGG	1 cut(s)	182
AgeI	ACCGGT	1 cut(s)	354
AgsI	TTSAA	8 cut(s)	209, 250, 502, 545, 650, 703, 733, 782
AhdI	GACNNNNNGTC	1 cut(s)	219
AjnI	CCWGG	1 cut(s)	720
AluBI	AGCT	3 cut(s)	40, 172, 709
AluI	AGCT	3 cut(s)	40, 172, 709
Alw26I	GTCTC	5 cut(s)	124, 206, 371, 600, 653
AlwI	GGATC	4 cut(s)	315, 428, 817, 830
AoxI	GGCC	1 cut(s)	101
ApeKI	GCWGC	1 cut(s)	706
ApoI	RAATTY	1 cut(s)	415
AsiGI	ACCGGT	1 cut(s)	354
Asp700I	GAANNNNTTC	1 cut(s)	645
AspS9I	GGNCC	3 cut(s)	102, 140, 718
AsuC2I	CCSGG	1 cut(s)	830
AsuHPI	GGTGA	1 cut(s)	394
AsuNHI	GCTAGC	1 cut(s)	40
AvaII	GGWCC	2 cut(s)	140, 718
BamHI	GGATCC	1 cut(s)	822
BanII	GRGCYC	1 cut(s)	835
BauI	CACGAG	1 cut(s)	558
BbvI	GCAGC	1 cut(s)	718
BccI	CCATC	4 cut(s)	279, 379, 782, 804
BceAI	ACGGC	2 cut(s)	512, 587
BcgI	CGANNNNNNTGC	2 cut(s)	257, 291
BciT130I	CCWGG	1 cut(s)	722
BciVI	GTATCC	1 cut(s)	792
BclI	TGATCA	2 cut(s)	319, 420
BcnI	CCSGG	1 cut(s)	830
BcoDI	GTCTC	5 cut(s)	124, 206, 371, 600, 653
BfaI	CTAG	4 cut(s)	41, 195, 227, 856
BfmI	CTRYAG	1 cut(s)	803
BfuI	GTATCC	1 cut(s)	792
BisI	GCNGC	1 cut(s)	707
BlsI	GCNGC	1 cut(s)	708
Bme1390I	CCNGG	2 cut(s)	722, 830
Bme18I	GGWCC	2 cut(s)	140, 718
BmeRI	GACNNNNNGTC	1 cut(s)	219
BmgT120I	GGNCC	3 cut(s)	102, 140, 718
BmiI	GGNNCC	1 cut(s)	824
BmrFI	CCNGG	2 cut(s)	722, 830
BmrI	ACTGGG	1 cut(s)	225
BmtI	GCTAGC	1 cut(s)	44
BmuI	ACTGGG	1 cut(s)	225
BpuEI	CTTGAG	1 cut(s)	264
BpuMI	CCSGG	1 cut(s)	830
BsaBI	GATNNNNATC	1 cut(s)	581
BsaHI	GRCGYC	1 cut(s)	220
BsaI	GGTCTC	1 cut(s)	124
BsaWI	WCCGGW	1 cut(s)	354
Bsc4I	CCNNNNNNNGG	1 cut(s)	182
Bse118I	RCCGGY	1 cut(s)	354
Bse1I	ACTGG	2 cut(s)	220, 336
Bse3DI	GCAATG	1 cut(s)	273
Bse8I	GATNNNNATC	1 cut(s)	581
BseBI	CCWGG	1 cut(s)	722
BseGI	GGATG	4 cut(s)	290, 474, 582, 815
BseJI	GATNNNNATC	1 cut(s)	581
BseLI	CCNNNNNNNGG	1 cut(s)	182
BseMI	GCAATG	1 cut(s)	273
BseMII	CTCAG	1 cut(s)	648
BseNI	ACTGG	2 cut(s)	220, 336
BseRI	GAGGAG	1 cut(s)	740
BseXI	GCAGC	1 cut(s)	718
BshFI	GGCC	1 cut(s)	103
BshTI	ACCGGT	1 cut(s)	354
BsiSI	CCGG	2 cut(s)	355, 829
BslFI	GGGAC	1 cut(s)	239
BslI	CCNNNNNNNGG	1 cut(s)	182
BsmAI	GTCTC	5 cut(s)	124, 206, 371, 600, 653
BsmBI	CGTCTC	1 cut(s)	371
BsmFI	GGGAC	1 cut(s)	239
BsnI	GGCC	1 cut(s)	103
Bso31I	GGTCTC	1 cut(s)	124
Bsp1286I	GDGCHC	1 cut(s)	835
Bsp1407I	TGTACA	1 cut(s)	471
Bsp143I	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
BspACI	CCGC	2 cut(s)	176, 574
BspANI	GGCC	1 cut(s)	103
BspCNI	CTCAG	1 cut(s)	649
BspHI	TCATGA	1 cut(s)	33
BspLI	GGNNCC	1 cut(s)	824
BspMAI	CTGCAG	1 cut(s)	807
BspOI	GCTAGC	1 cut(s)	44
BspPI	GGATC	4 cut(s)	315, 428, 817, 830
BspTNI	GGTCTC	1 cut(s)	124
BsrDI	GCAATG	1 cut(s)	273
BsrFI	RCCGGY	1 cut(s)	354
BsrGI	TGTACA	1 cut(s)	471
BsrI	ACTGG	2 cut(s)	220, 336
BssAI	RCCGGY	1 cut(s)	354
BssMI	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
BssNI	GRCGYC	1 cut(s)	220
BssSI	CACGAG	1 cut(s)	558
Bst2BI	CACGAG	1 cut(s)	558
Bst2UI	CCWGG	1 cut(s)	722
Bst4CI	ACNGT	4 cut(s)	382, 409, 498, 553
Bst6I	CTCTTC	1 cut(s)	204
BstACI	GRCGYC	1 cut(s)	220
BstAUI	TGTACA	1 cut(s)	471
BstC8I	GCNNGC	2 cut(s)	42, 628
BstDEI	CTNAG	1 cut(s)	657
BstF5I	GGATG	4 cut(s)	290, 474, 582, 815
BstKTI	GATC	6 cut(s)	310, 322, 423, 436, 681, 825
BstMAI	GTCTC	5 cut(s)	124, 206, 371, 600, 653
BstMBI	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
BstMWI	GCNNNNNNNGC	1 cut(s)	26
BstNI	CCWGG	1 cut(s)	722
BstNSI	RCATGY	1 cut(s)	630
BstSCI	CCNGG	2 cut(s)	720, 828
BstSFI	CTRYAG	1 cut(s)	803
BstV1I	GCAGC	1 cut(s)	718
BstX2I	RGATCY	3 cut(s)	307, 433, 822
BstYI	RGATCY	3 cut(s)	307, 433, 822
BsuI	GTATCC	1 cut(s)	792
BsuRI	GGCC	1 cut(s)	103
BtsCI	GGATG	4 cut(s)	290, 474, 582, 815
BtsI	GCAGTG	1 cut(s)	800
BtsIMutI	CAGTG	1 cut(s)	800
Cac8I	GCNNGC	2 cut(s)	42, 628
CciI	TCATGA	1 cut(s)	33
Cfr10I	RCCGGY	1 cut(s)	354
Cfr13I	GGNCC	3 cut(s)	102, 140, 718
CseI	GACGC	1 cut(s)	209
Csp6I	GTAC	2 cut(s)	265, 472
CspAI	ACCGGT	1 cut(s)	354
CviAII	CATG	4 cut(s)	34, 371, 452, 627
CviJI	RGCY	7 cut(s)	40, 61, 103, 172, 695, 709, 833
CviKI_1	RGCY	7 cut(s)	40, 61, 103, 172, 695, 709, 833
CviQI	GTAC	2 cut(s)	265, 472
DdeI	CTNAG	1 cut(s)	657
DpnI	GATC	6 cut(s)	309, 321, 422, 435, 680, 824
DpnII	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
DriI	GACNNNNNGTC	1 cut(s)	219
Eam1104I	CTCTTC	1 cut(s)	204
Eam1105I	GACNNNNNGTC	1 cut(s)	219
EarI	CTCTTC	1 cut(s)	204
EciI	GGCGGA	1 cut(s)	589
Eco24I	GRGCYC	1 cut(s)	835
Eco31I	GGTCTC	1 cut(s)	124
Eco47I	GGWCC	2 cut(s)	140, 718
EcoRII	CCWGG	1 cut(s)	720
EcoT38I	GRGCYC	1 cut(s)	835
Esp3I	CGTCTC	1 cut(s)	371
FaeI	CATG	4 cut(s)	37, 374, 455, 630
FaqI	GGGAC	1 cut(s)	239
FatI	CATG	4 cut(s)	33, 370, 451, 626
FbaI	TGATCA	2 cut(s)	319, 420
Fnu4HI	GCNGC	1 cut(s)	707
FokI	GGATG	4 cut(s)	297, 481, 589, 822
FriOI	GRGCYC	1 cut(s)	835
Fsp4HI	GCNGC	1 cut(s)	707
FspBI	CTAG	4 cut(s)	41, 195, 227, 856
GluI	GCNGC	1 cut(s)	707
HaeIII	GGCC	1 cut(s)	103
HapII	CCGG	2 cut(s)	355, 829
HgaI	GACGC	1 cut(s)	209
Hin1I	GRCGYC	1 cut(s)	220
Hin1II	CATG	4 cut(s)	37, 374, 455, 630
HinfI	GANTC	1 cut(s)	847
HpaII	CCGG	2 cut(s)	355, 829
HphI	GGTGA	1 cut(s)	394
Hpy188I	TCNGA	3 cut(s)	127, 351, 399
Hpy188III	TCNNGA	7 cut(s)	34, 412, 424, 437, 560, 586, 682
Hpy99I	CGWCG	1 cut(s)	225
HpyAV	CCTTC	3 cut(s)	115, 493, 813
HpyCH4III	ACNGT	4 cut(s)	382, 409, 498, 553
HpyCH4IV	ACGT	1 cut(s)	181
HpyCH4V	TGCA	3 cut(s)	242, 517, 805
HpyF10VI	GCNNNNNNNGC	1 cut(s)	26
HpyF3I	CTNAG	1 cut(s)	657
HpySE526I	ACGT	1 cut(s)	181
Hsp92I	GRCGYC	1 cut(s)	220
Hsp92II	CATG	4 cut(s)	37, 374, 455, 630
Ksp22I	TGATCA	2 cut(s)	319, 420
Kzo9I	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
LmnI	GCTCC	1 cut(s)	714
Lsp1109I	GCAGC	1 cut(s)	718
MaeI	CTAG	4 cut(s)	41, 195, 227, 856
MaeII	ACGT	1 cut(s)	181
MaeIII	GTNAC	3 cut(s)	6, 150, 560
MalI	GATC	6 cut(s)	309, 321, 422, 435, 680, 824
MboI	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
MboII	GAAGA	4 cut(s)	221, 745, 794, 797
MflI	RGATCY	3 cut(s)	307, 433, 822
MhlI	GDGCHC	1 cut(s)	835
MluCI	AATT	5 cut(s)	11, 165, 315, 343, 415
MlyI	GAGTC	1 cut(s)	841
MmeI	TCCRAC	2 cut(s)	139, 696
MnlI	CCTC	9 cut(s)	153, 298, 499, 567, 625, 684, 718, 735, 836
MroXI	GAANNNNTTC	1 cut(s)	645
MseI	TTAA	2 cut(s)	312, 359
MslI	CAYNNNNRTG	2 cut(s)	369, 522
MspI	CCGG	2 cut(s)	355, 829
MspR9I	CCNGG	2 cut(s)	722, 830
MvaI	CCWGG	1 cut(s)	722
MwoI	GCNNNNNNNGC	1 cut(s)	26
NciI	CCSGG	1 cut(s)	830
NdeII	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
NheI	GCTAGC	1 cut(s)	40
NlaIII	CATG	4 cut(s)	37, 374, 455, 630
NlaIV	GGNNCC	1 cut(s)	824
NmuCI	GTSAC	2 cut(s)	6, 560
NspI	RCATGY	1 cut(s)	630
PaeI	GCATGC	1 cut(s)	630
PagI	TCATGA	1 cut(s)	33
PcsI	WCGNNNNNNNCGW	2 cut(s)	187, 627
PdmI	GAANNNNTTC	1 cut(s)	645
PinAI	ACCGGT	1 cut(s)	354
PkrI	GCNGC	1 cut(s)	708
PleI	GAGTC	1 cut(s)	841
PpsI	GAGTC	1 cut(s)	841
Psp6I	CCWGG	1 cut(s)	720
PspGI	CCWGG	1 cut(s)	720
PspN4I	GGNNCC	1 cut(s)	824
PspPI	GGNCC	3 cut(s)	102, 140, 718
PstI	CTGCAG	1 cut(s)	807
PsuI	RGATCY	3 cut(s)	307, 433, 822
RsaI	GTAC	2 cut(s)	266, 473
RsaNI	GTAC	2 cut(s)	265, 472
RseI	CAYNNNNRTG	2 cut(s)	369, 522
SaqAI	TTAA	2 cut(s)	312, 359
SatI	GCNGC	1 cut(s)	707
Sau3AI	GATC	6 cut(s)	307, 319, 420, 433, 678, 822
Sau96I	GGNCC	3 cut(s)	102, 140, 718
SchI	GAGTC	1 cut(s)	841
ScrFI	CCNGG	2 cut(s)	722, 830
SduI	GDGCHC	1 cut(s)	835
SfcI	CTRYAG	1 cut(s)	803
SinI	GGWCC	2 cut(s)	140, 718
SmiMI	CAYNNNNRTG	2 cut(s)	369, 522
SmlI	CTYRAG	1 cut(s)	279
SmoI	CTYRAG	1 cut(s)	279
SphI	GCATGC	1 cut(s)	630
Sse9I	AATT	5 cut(s)	11, 165, 315, 343, 415
SsiI	CCGC	2 cut(s)	176, 574
SspMI	CTAG	4 cut(s)	41, 195, 227, 856
StyD4I	CCNGG	2 cut(s)	720, 828
TaaI	ACNGT	4 cut(s)	382, 409, 498, 553
TaiI	ACGT	1 cut(s)	184
TaqI	TCGA	2 cut(s)	190, 585
TaqII	GACCGA	1 cut(s)	148
TasI	AATT	5 cut(s)	11, 165, 315, 343, 415
TatI	WGTACW	1 cut(s)	471
Tru1I	TTAA	2 cut(s)	312, 359
Tru9I	TTAA	2 cut(s)	312, 359
TscAI	CASTG	1 cut(s)	800
TseFI	GTSAC	2 cut(s)	6, 560
TseI	GCWGC	1 cut(s)	706
Tsp45I	GTSAC	2 cut(s)	6, 560
TspDTI	ATGAA	6 cut(s)	50, 66, 436, 440, 582, 629
TspRI	CASTG	1 cut(s)	800
VpaK11BI	GGWCC	2 cut(s)	140, 718
XapI	RAATTY	1 cut(s)	415
XceI	RCATGY	1 cut(s)	630
XmnI	GAANNNNTTC	1 cut(s)	645
XspI	CTAG	4 cut(s)	41, 195, 227, 856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.