Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 867 |
| AciI | CCGC | 2 cut(s) | 700, 867 |
| AclWI | GGATC | 7 cut(s) | 87, 119, 445, 682, 894, 907, 1309 |
| AcsI | RAATTY | 4 cut(s) | 21, 41, 513, 1213 |
| AcuI | CTGAAG | 4 cut(s) | 169, 345, 366, 1383 |
| AcyI | GRCGYC | 1 cut(s) | 1426 |
| AdeI | CACNNNGTG | 1 cut(s) | 671 |
| AfaI | GTAC | 3 cut(s) | 239, 795, 1556 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 412, 413, 1385 |
| AflIII | ACRYGT | 1 cut(s) | 1737 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1593 |
| AjnI | CCWGG | 4 cut(s) | 339, 1384, 1428, 1920 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1374, 1406 |
| Alw21I | GWGCWC | 2 cut(s) | 855, 985 |
| Alw26I | GTCTC | 3 cut(s) | 828, 1742, 1853 |
| Alw44I | GTGCAC | 1 cut(s) | 981 |
| AlwI | GGATC | 7 cut(s) | 87, 119, 445, 682, 894, 907, 1309 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1154 |
| Ama87I | CYCGRG | 1 cut(s) | 1758 |
| AoxI | GGCC | 7 cut(s) | 69, 303, 1099, 1304, 1321, 1394, 1853 |
| ApaLI | GTGCAC | 1 cut(s) | 981 |
| ApeKI | GCWGC | 8 cut(s) | 289, 314, 446, 588, 877, 1042, 1118, 1151 |
| ApoI | RAATTY | 4 cut(s) | 21, 41, 513, 1213 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 41, 1497 |
| AspS9I | GGNCC | 4 cut(s) | 304, 1343, 1668, 1918 |
| AsuC2I | CCSGG | 2 cut(s) | 68, 1703 |
| AsuHPI | GGTGA | 3 cut(s) | 657, 821, 1073 |
| AsuNHI | GCTAGC | 1 cut(s) | 449 |
| AvaI | CYCGRG | 1 cut(s) | 1758 |
| AvaII | GGWCC | 3 cut(s) | 1343, 1668, 1918 |
| AxyI | CCTNAGG | 1 cut(s) | 840 |
| BaeGI | GKGCMC | 1 cut(s) | 985 |
| BamHI | GGATCC | 1 cut(s) | 899 |
| BbsI | GAAGAC | 1 cut(s) | 711 |
| Bbv12I | GWGCWC | 2 cut(s) | 855, 985 |
| BbvI | GCAGC | 8 cut(s) | 276, 301, 433, 600, 889, 1054, 1105, 1138 |
| BccI | CCATC | 4 cut(s) | 647, 1058, 1985, 2007 |
| BciT130I | CCWGG | 4 cut(s) | 341, 1386, 1430, 1922 |
| BciVI | GTATCC | 3 cut(s) | 363, 1289, 1597 |
| BclI | TGATCA | 2 cut(s) | 52, 1620 |
| BcnI | CCSGG | 2 cut(s) | 68, 1703 |
| BcoDI | GTCTC | 3 cut(s) | 828, 1742, 1853 |
| BfaI | CTAG | 4 cut(s) | 140, 159, 450, 1244 |
| BfmI | CTRYAG | 3 cut(s) | 290, 603, 1119 |
| BfuI | GTATCC | 3 cut(s) | 363, 1289, 1597 |
| BglII | AGATCT | 1 cut(s) | 1926 |
| BisI | GCNGC | 8 cut(s) | 290, 315, 447, 589, 878, 1043, 1119, 1152 |
| BlsI | GCNGC | 8 cut(s) | 291, 316, 448, 590, 879, 1044, 1120, 1153 |
| Bme1390I | CCNGG | 6 cut(s) | 68, 341, 1386, 1430, 1703, 1922 |
| Bme18I | GGWCC | 3 cut(s) | 1343, 1668, 1918 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1593 |
| BmeT110I | CYCGRG | 1 cut(s) | 1758 |
| BmgT120I | GGNCC | 4 cut(s) | 304, 1343, 1668, 1918 |
| BmiI | GGNNCC | 3 cut(s) | 901, 1069, 1669 |
| BmrFI | CCNGG | 6 cut(s) | 68, 341, 1386, 1430, 1703, 1922 |
| BmrI | ACTGGG | 3 cut(s) | 21, 123, 466 |
| BmsI | GCATC | 1 cut(s) | 240 |
| BmtI | GCTAGC | 1 cut(s) | 453 |
| BmuI | ACTGGG | 3 cut(s) | 21, 123, 466 |
| BpiI | GAAGAC | 1 cut(s) | 711 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 341, 373 |
| BpmI | CTGGAG | 2 cut(s) | 189, 1386 |
| Bpu10I | CCTNAGC | 1 cut(s) | 624 |
| BpuEI | CTTGAG | 2 cut(s) | 218, 1183 |
| BpuMI | CCSGG | 2 cut(s) | 68, 1703 |
| BsaHI | GRCGYC | 1 cut(s) | 1426 |
| BsaJI | CCNNGG | 3 cut(s) | 611, 1318, 1598 |
| BsaWI | WCCGGW | 1 cut(s) | 931 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 251, 281, 514, 544 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 412, 413, 1385 |
| Bse1I | ACTGG | 6 cut(s) | 27, 129, 461, 1369, 1920, 2051 |
| Bse21I | CCTNAGG | 1 cut(s) | 840 |
| BseBI | CCWGG | 4 cut(s) | 341, 1386, 1430, 1922 |
| BseDI | CCNNGG | 3 cut(s) | 611, 1318, 1598 |
| BseGI | GGATG | 6 cut(s) | 22, 634, 940, 1813, 1996, 2018 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 412, 413, 1385 |
| BseMII | CTCAG | 8 cut(s) | 87, 204, 480, 759, 840, 854, 1042, 1848 |
| BseNI | ACTGG | 6 cut(s) | 27, 129, 461, 1369, 1920, 2051 |
| BseRI | GAGGAG | 4 cut(s) | 160, 745, 828, 1760 |
| BseSI | GKGCMC | 1 cut(s) | 985 |
| BseXI | GCAGC | 8 cut(s) | 276, 301, 433, 600, 889, 1054, 1105, 1138 |
| BseYI | CCCAGC | 2 cut(s) | 1071, 1895 |
| BshFI | GGCC | 7 cut(s) | 71, 305, 1101, 1306, 1323, 1396, 1855 |
| BsiHKAI | GWGCWC | 2 cut(s) | 855, 985 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1758 |
| BsiSI | CCGG | 3 cut(s) | 68, 932, 1702 |
| BslFI | GGGAC | 2 cut(s) | 50, 1442 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 412, 413, 1385 |
| BsmAI | GTCTC | 3 cut(s) | 828, 1742, 1853 |
| BsmFI | GGGAC | 2 cut(s) | 50, 1442 |
| BsnI | GGCC | 7 cut(s) | 71, 305, 1101, 1306, 1323, 1396, 1855 |
| BsoBI | CYCGRG | 1 cut(s) | 1758 |
| Bsp1286I | GDGCHC | 2 cut(s) | 855, 985 |
| BspACI | CCGC | 2 cut(s) | 700, 867 |
| BspANI | GGCC | 7 cut(s) | 71, 305, 1101, 1306, 1323, 1396, 1855 |
| BspCNI | CTCAG | 8 cut(s) | 88, 203, 479, 760, 841, 853, 1041, 1849 |
| BspHI | TCATGA | 1 cut(s) | 112 |
| BspLI | GGNNCC | 3 cut(s) | 901, 1069, 1669 |
| BspMAI | CTGCAG | 2 cut(s) | 294, 1123 |
| BspOI | GCTAGC | 1 cut(s) | 453 |
| BspPI | GGATC | 7 cut(s) | 87, 119, 445, 682, 894, 907, 1309 |
| BsrBI | CCGCTC | 1 cut(s) | 867 |
| BsrI | ACTGG | 6 cut(s) | 27, 129, 461, 1369, 1920, 2051 |
| BssECI | CCNNGG | 3 cut(s) | 611, 1318, 1598 |
| BssNI | GRCGYC | 1 cut(s) | 1426 |
| BssT1I | CCWWGG | 3 cut(s) | 611, 1318, 1598 |
| Bst2UI | CCWGG | 4 cut(s) | 341, 1386, 1430, 1922 |
| Bst4CI | ACNGT | 8 cut(s) | 212, 299, 783, 1061, 1585, 1595, 1609, 1731 |
| Bst6I | CTCTTC | 5 cut(s) | 258, 364, 1054, 1718, 1729 |
| BstACI | GRCGYC | 1 cut(s) | 1426 |
| BstC8I | GCNNGC | 5 cut(s) | 287, 451, 621, 997, 1192 |
| BstF5I | GGATG | 6 cut(s) | 22, 634, 940, 1813, 1996, 2018 |
| BstMAI | GTCTC | 3 cut(s) | 828, 1742, 1853 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 286, 311, 755, 1030, 1039, 1244, 1253 |
| BstNI | CCWGG | 4 cut(s) | 341, 1386, 1430, 1922 |
| BstNSI | RCATGY | 5 cut(s) | 289, 1194, 1741, 1830, 1999 |
| BstSCI | CCNGG | 6 cut(s) | 66, 339, 1384, 1428, 1701, 1920 |
| BstSFI | CTRYAG | 3 cut(s) | 290, 603, 1119 |
| BstSLI | GKGCMC | 1 cut(s) | 985 |
| BstV1I | GCAGC | 8 cut(s) | 276, 301, 433, 600, 889, 1054, 1105, 1138 |
| BstV2I | GAAGAC | 1 cut(s) | 711 |
| BstX2I | RGATCY | 4 cut(s) | 124, 437, 899, 1926 |
| BstYI | RGATCY | 4 cut(s) | 124, 437, 899, 1926 |
| Bsu36I | CCTNAGG | 1 cut(s) | 840 |
| BsuI | GTATCC | 3 cut(s) | 363, 1289, 1597 |
| BsuRI | GGCC | 7 cut(s) | 71, 305, 1101, 1306, 1323, 1396, 1855 |
| BtsCI | GGATG | 6 cut(s) | 22, 634, 940, 1813, 1996, 2018 |
| BtsI | GCAGTG | 2 cut(s) | 965, 1877 |
| BtsIMutI | CAGTG | 5 cut(s) | 965, 1590, 1727, 1877, 1913 |
| Cac8I | GCNNGC | 5 cut(s) | 287, 451, 621, 997, 1192 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1154 |
| CciI | TCATGA | 1 cut(s) | 112 |
| Cfr13I | GGNCC | 4 cut(s) | 304, 1343, 1668, 1918 |
| CseI | GACGC | 1 cut(s) | 1434 |
| CsiI | ACCWGGT | 1 cut(s) | 1384 |
| Csp6I | GTAC | 3 cut(s) | 238, 794, 1555 |
| CviQI | GTAC | 3 cut(s) | 238, 794, 1555 |
| DraI | TTTAAA | 1 cut(s) | 1453 |
| DraIII | CACNNNGTG | 1 cut(s) | 671 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1593 |
| Eam1104I | CTCTTC | 5 cut(s) | 258, 364, 1054, 1718, 1729 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1593 |
| EarI | CTCTTC | 5 cut(s) | 258, 364, 1054, 1718, 1729 |
| Eco130I | CCWWGG | 3 cut(s) | 611, 1318, 1598 |
| Eco147I | AGGCCT | 2 cut(s) | 1323, 1855 |
| Eco32I | GATATC | 1 cut(s) | 1513 |
| Eco47I | GGWCC | 3 cut(s) | 1343, 1668, 1918 |
| Eco57I | CTGAAG | 4 cut(s) | 169, 345, 366, 1383 |
| Eco81I | CCTNAGG | 1 cut(s) | 840 |
| Eco88I | CYCGRG | 1 cut(s) | 1758 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1668 |
| EcoRII | CCWGG | 4 cut(s) | 339, 1384, 1428, 1920 |
| EcoRV | GATATC | 1 cut(s) | 1513 |
| EcoT14I | CCWWGG | 3 cut(s) | 611, 1318, 1598 |
| ErhI | CCWWGG | 3 cut(s) | 611, 1318, 1598 |
| FaqI | GGGAC | 2 cut(s) | 50, 1442 |
| FauI | CCCGC | 1 cut(s) | 693 |
| FbaI | TGATCA | 2 cut(s) | 52, 1620 |
| Fnu4HI | GCNGC | 8 cut(s) | 290, 315, 447, 589, 878, 1043, 1119, 1152 |
| FokI | GGATG | 6 cut(s) | 29, 641, 947, 1820, 2003, 2025 |
| Fsp4HI | GCNGC | 8 cut(s) | 290, 315, 447, 589, 878, 1043, 1119, 1152 |
| FspBI | CTAG | 4 cut(s) | 140, 159, 450, 1244 |
| GluI | GCNGC | 8 cut(s) | 290, 315, 447, 589, 878, 1043, 1119, 1152 |
| GsaI | CCCAGC | 2 cut(s) | 1075, 1899 |
| GsuI | CTGGAG | 2 cut(s) | 189, 1386 |
| HaeIII | GGCC | 7 cut(s) | 71, 305, 1101, 1306, 1323, 1396, 1855 |
| HapII | CCGG | 3 cut(s) | 68, 932, 1702 |
| HgaI | GACGC | 1 cut(s) | 1434 |
| Hin1I | GRCGYC | 1 cut(s) | 1426 |
| HincII | GTYRAC | 1 cut(s) | 1984 |
| HindII | GTYRAC | 1 cut(s) | 1984 |
| HinfI | GANTC | 6 cut(s) | 200, 527, 764, 821, 845, 1750 |
| HpaII | CCGG | 3 cut(s) | 68, 932, 1702 |
| HphI | GGTGA | 3 cut(s) | 657, 821, 1073 |
| Hpy166II | GTNNAC | 4 cut(s) | 388, 983, 1343, 1984 |
| Hpy188I | TCNGA | 8 cut(s) | 79, 84, 193, 469, 769, 850, 1402, 1960 |
| Hpy8I | GTNNAC | 4 cut(s) | 388, 983, 1343, 1984 |
| Hpy99I | CGWCG | 2 cut(s) | 713, 1428 |
| HpyAV | CCTTC | 6 cut(s) | 165, 719, 1207, 1407, 1580, 1693 |
| HpyCH4III | ACNGT | 8 cut(s) | 212, 299, 783, 1061, 1585, 1595, 1609, 1731 |
| HpyCH4IV | ACGT | 1 cut(s) | 1955 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 286, 311, 755, 1030, 1039, 1244, 1253 |
| HpySE526I | ACGT | 1 cut(s) | 1955 |
| Hsp92I | GRCGYC | 1 cut(s) | 1426 |
| Ksp22I | TGATCA | 2 cut(s) | 52, 1620 |
| LmnI | GCTCC | 4 cut(s) | 616, 681, 732, 864 |
| Lsp1109I | GCAGC | 8 cut(s) | 276, 301, 433, 600, 889, 1054, 1105, 1138 |
| LweI | GCATC | 1 cut(s) | 240 |
| MabI | ACCWGGT | 1 cut(s) | 1384 |
| MaeI | CTAG | 4 cut(s) | 140, 159, 450, 1244 |
| MaeII | ACGT | 1 cut(s) | 1955 |
| MaeIII | GTNAC | 5 cut(s) | 91, 419, 1585, 1908, 1951 |
| MbiI | CCGCTC | 1 cut(s) | 867 |
| MfeI | CAATTG | 2 cut(s) | 1233, 1546 |
| MflI | RGATCY | 4 cut(s) | 124, 437, 899, 1926 |
| MhlI | GDGCHC | 2 cut(s) | 855, 985 |
| MlyI | GAGTC | 2 cut(s) | 830, 1744 |
| MmeI | TCCRAC | 2 cut(s) | 249, 1353 |
| MroXI | GAANNNNTTC | 2 cut(s) | 41, 1497 |
| MseI | TTAA | 3 cut(s) | 882, 1452, 1632 |
| MspA1I | CMGCKG | 3 cut(s) | 700, 749, 1151 |
| MspI | CCGG | 3 cut(s) | 68, 932, 1702 |
| MspR9I | CCNGG | 6 cut(s) | 68, 341, 1386, 1430, 1703, 1922 |
| MunI | CAATTG | 2 cut(s) | 1233, 1546 |
| MvaI | CCWGG | 4 cut(s) | 341, 1386, 1430, 1922 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 286, 311, 755, 1030, 1039, 1244, 1253 |
| NciI | CCSGG | 2 cut(s) | 68, 1703 |
| NheI | GCTAGC | 1 cut(s) | 449 |
| NlaIV | GGNNCC | 3 cut(s) | 901, 1069, 1669 |
| NmuCI | GTSAC | 1 cut(s) | 1585 |
| NspI | RCATGY | 5 cut(s) | 289, 1194, 1741, 1830, 1999 |
| PaeI | GCATGC | 2 cut(s) | 289, 1194 |
| PagI | TCATGA | 1 cut(s) | 112 |
| PceI | AGGCCT | 2 cut(s) | 1323, 1855 |
| PciI | ACATGT | 1 cut(s) | 1737 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1740, 1983 |
| PdmI | GAANNNNTTC | 2 cut(s) | 41, 1497 |
| PfeI | GAWTC | 4 cut(s) | 200, 527, 764, 845 |
| PfoI | TCCNGGA | 1 cut(s) | 1701 |
| PkrI | GCNGC | 8 cut(s) | 291, 316, 448, 590, 879, 1044, 1120, 1153 |
| PleI | GAGTC | 2 cut(s) | 829, 1744 |
| PpsI | GAGTC | 2 cut(s) | 829, 1744 |
| PpuMI | RGGWCCY | 1 cut(s) | 1668 |
| PscI | ACATGT | 1 cut(s) | 1737 |
| Psp5II | RGGWCCY | 1 cut(s) | 1668 |
| Psp6I | CCWGG | 4 cut(s) | 339, 1384, 1428, 1920 |
| PspFI | CCCAGC | 2 cut(s) | 1071, 1895 |
| PspGI | CCWGG | 4 cut(s) | 339, 1384, 1428, 1920 |
| PspN4I | GGNNCC | 3 cut(s) | 901, 1069, 1669 |
| PspPI | GGNCC | 4 cut(s) | 304, 1343, 1668, 1918 |
| PspPPI | RGGWCCY | 1 cut(s) | 1668 |
| PstI | CTGCAG | 2 cut(s) | 294, 1123 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1154 |
| PsuI | RGATCY | 4 cut(s) | 124, 437, 899, 1926 |
| PvuII | CAGCTG | 2 cut(s) | 749, 1151 |
| RsaI | GTAC | 3 cut(s) | 239, 795, 1556 |
| RsaNI | GTAC | 3 cut(s) | 238, 794, 1555 |
| SaqAI | TTAA | 3 cut(s) | 882, 1452, 1632 |
| SatI | GCNGC | 8 cut(s) | 290, 315, 447, 589, 878, 1043, 1119, 1152 |
| Sau96I | GGNCC | 4 cut(s) | 304, 1343, 1668, 1918 |
| SchI | GAGTC | 2 cut(s) | 830, 1744 |
| ScrFI | CCNGG | 6 cut(s) | 68, 341, 1386, 1430, 1703, 1922 |
| SduI | GDGCHC | 2 cut(s) | 855, 985 |
| SexAI | ACCWGGT | 1 cut(s) | 1384 |
| SfaNI | GCATC | 1 cut(s) | 240 |
| SfcI | CTRYAG | 3 cut(s) | 290, 603, 1119 |
| SinI | GGWCC | 3 cut(s) | 1343, 1668, 1918 |
| SmlI | CTYRAG | 2 cut(s) | 233, 1162 |
| SmoI | CTYRAG | 2 cut(s) | 233, 1162 |
| SphI | GCATGC | 2 cut(s) | 289, 1194 |
| SseBI | AGGCCT | 2 cut(s) | 1323, 1855 |
| SsiI | CCGC | 2 cut(s) | 700, 867 |
| SspI | AATATT | 1 cut(s) | 88 |
| SspMI | CTAG | 4 cut(s) | 140, 159, 450, 1244 |
| StuI | AGGCCT | 2 cut(s) | 1323, 1855 |
| StyD4I | CCNGG | 6 cut(s) | 66, 339, 1384, 1428, 1701, 1920 |
| StyI | CCWWGG | 3 cut(s) | 611, 1318, 1598 |
| TaaI | ACNGT | 8 cut(s) | 212, 299, 783, 1061, 1585, 1595, 1609, 1731 |
| TaiI | ACGT | 1 cut(s) | 1958 |
| TaqI | TCGA | 4 cut(s) | 559, 1689, 1734, 1743 |
| TatI | WGTACW | 1 cut(s) | 237 |
| TfiI | GAWTC | 4 cut(s) | 200, 527, 764, 845 |
| Tru1I | TTAA | 3 cut(s) | 882, 1452, 1632 |
| Tru9I | TTAA | 3 cut(s) | 882, 1452, 1632 |
| TscAI | CASTG | 5 cut(s) | 972, 1590, 1734, 1877, 1920 |
| TseFI | GTSAC | 1 cut(s) | 1585 |
| TseI | GCWGC | 8 cut(s) | 289, 314, 446, 588, 877, 1042, 1118, 1151 |
| Tsp45I | GTSAC | 1 cut(s) | 1585 |
| TspDTI | ATGAA | 8 cut(s) | 33, 34, 50, 738, 777, 1593, 1640, 2021 |
| TspRI | CASTG | 5 cut(s) | 972, 1590, 1734, 1877, 1920 |
| VneI | GTGCAC | 1 cut(s) | 981 |
| VpaK11BI | GGWCC | 3 cut(s) | 1343, 1668, 1918 |
| XapI | RAATTY | 4 cut(s) | 21, 41, 513, 1213 |
| XceI | RCATGY | 5 cut(s) | 289, 1194, 1741, 1830, 1999 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 431 |
| XmnI | GAANNNNTTC | 2 cut(s) | 41, 1497 |
| XspI | CTAG | 4 cut(s) | 140, 159, 450, 1244 |