Rmu_co8258053.1_g000001

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8258053.1
OG0000175
Physical Location & Seq
Reverse (-)
75 .. 803
729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8258053.1_g000001.1.cds

Sequence Viewer

Length: 729 bp
ggtggacctcctcagatacaaatcacagcaattgagcttccccaaccaggttttcgacctacagaaagggttgaagagaccgggcgtcgtctagcaaggtattgtgcaaagttcaatgtcccgtttgagtacaatgtcattgcacagaaatgggaatctatcagagttgaggatcttaatattgacagaaatgagctcatcgtggtgaattgtatgcaccggttaaggcacatacctgatgagacagtgatggtgagcagtccaagagacagagtactaaagttgattaagaaaattaatccagaccttttcgttcatgggattatcaatgggacatacagtccacccttttttgtcacgcggttcaaagaggcactcttccatttcggtgcactgtttgatatgtttgaggccattgttcctcgtgaagatcaatggaggcagatgttcgaaagagaaatatatgggagggacataataaatgttgtagcatgcgagggactggaaagagttgaaaggcccgagacatacaagcaatggaatctcaggactctcaggactgggttcaagcaggtgccattagagcaggattgcttgatgaaagtgaagagtatgttaaagttgacgagttatcataaggactttagaattgatgaagatggggagtggatgctgcagggatggaaaggaagaatcgttatggctctctctttttggaaacctgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

242

Amino Acids

28.6

Weight (kDa)

8.64

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 562
Acc36I ACCTGC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 574
AccII CGCG 1 cut(s) 361
AciI CCGC 1 cut(s) 361
AclWI GGATC 1 cut(s) 180
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 2 cut(s) 131, 276
AfiI CCNNNNNNNGG 1 cut(s) 47
AgeI ACCGGT 1 cut(s) 219
AgsI TTSAA 5 cut(s) 74, 115, 367, 515, 568
AhdI GACNNNNNGTC 2 cut(s) 84, 339
AjnI CCWGG 1 cut(s) 46
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AluBI AGCT 2 cut(s) 37, 196
AluI AGCT 2 cut(s) 37, 196
Alw21I GWGCWC 2 cut(s) 198, 394
Alw26I GTCTC 4 cut(s) 71, 236, 261, 518
Alw44I GTGCAC 1 cut(s) 390
AlwI GGATC 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 2 cut(s) 411, 518
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 673
AseI ATTAAT 1 cut(s) 297
AsiGI ACCGGT 1 cut(s) 219
AspS9I GGNCC 2 cut(s) 5, 519
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 217, 265
AsuII TTCGAA 1 cut(s) 450
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 198, 394
BbvI GCAGC 1 cut(s) 660
BccI CCATC 3 cut(s) 244, 653, 675
BciT130I CCWGG 1 cut(s) 48
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 4 cut(s) 71, 236, 261, 518
BfaI CTAG 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 60, 674
BfuAI ACCTGC 1 cut(s) 562
BisI GCNGC 1 cut(s) 674
BlsI GCNGC 1 cut(s) 675
BmcAI AGTACT 1 cut(s) 276
Bme1390I CCNGG 2 cut(s) 48, 82
Bme18I GGWCC 1 cut(s) 5
BmeRI GACNNNNNGTC 2 cut(s) 84, 339
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 2 cut(s) 5, 519
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 2 cut(s) 48, 82
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 660
BmuI ACTGGG 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 450
BpuMI CCSGG 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 85
BsaI GGTCTC 1 cut(s) 71
BsaWI WCCGGW 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 2 cut(s) 507, 565
Bse3DI GCAATG 2 cut(s) 138, 542
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 675, 686
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 2 cut(s) 138, 542
BseMII CTCAG 3 cut(s) 26, 559, 568
BseNI ACTGG 2 cut(s) 507, 565
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 1 cut(s) 660
Bsh1236I CGCG 1 cut(s) 361
BshFI GGCC 2 cut(s) 413, 520
BshNI GGYRCC 1 cut(s) 574
BshTI ACCGGT 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 198, 394
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 2 cut(s) 81, 220
BslFI GGGAC 4 cut(s) 104, 346, 485, 513
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 4 cut(s) 71, 236, 261, 518
BsmFI GGGAC 4 cut(s) 104, 346, 485, 513
BsnI GGCC 2 cut(s) 413, 520
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 521
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 2 cut(s) 198, 394
Bsp143I GATC 2 cut(s) 172, 430
BspACI CCGC 1 cut(s) 361
BspANI GGCC 2 cut(s) 413, 520
BspCNI CTCAG 3 cut(s) 25, 558, 567
BspFNI CGCG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 576
BspMAI CTGCAG 1 cut(s) 678
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 1 cut(s) 180
BspT104I TTCGAA 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 574
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 138, 542
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 2 cut(s) 507, 565
BssAI RCCGGY 1 cut(s) 219
BssMI GATC 2 cut(s) 172, 430
BssNI GRCGYC 1 cut(s) 85
BssSI CACGAG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 247, 341, 396
Bst6I CTCTTC 3 cut(s) 69, 383, 602
BstACI GRCGYC 1 cut(s) 85
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 1 cut(s) 493
BstDEI CTNAG 3 cut(s) 12, 545, 554
BstF5I GGATG 2 cut(s) 675, 686
BstFNI CGCG 1 cut(s) 361
BstKTI GATC 2 cut(s) 175, 433
BstMAI GTCTC 4 cut(s) 71, 236, 261, 518
BstMBI GATC 2 cut(s) 172, 430
BstMWI GCNNNNNNNGC 1 cut(s) 583
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 495
BstSCI CCNGG 2 cut(s) 46, 80
BstSFI CTRYAG 2 cut(s) 60, 674
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 1 cut(s) 660
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuRI GGCC 2 cut(s) 413, 520
BtsCI GGATG 2 cut(s) 675, 686
BtsIMutI CAGTG 2 cut(s) 252, 392
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 493
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 5, 519
CseI GACGC 1 cut(s) 74
CsiI ACCWGGT 1 cut(s) 46
Csp6I GTAC 2 cut(s) 130, 275
CspAI ACCGGT 1 cut(s) 219
CviAII CATG 2 cut(s) 317, 492
CviJI RGCY 5 cut(s) 37, 196, 413, 520, 704
CviKI_1 RGCY 5 cut(s) 37, 196, 413, 520, 704
CviQI GTAC 2 cut(s) 130, 275
DdeI CTNAG 3 cut(s) 12, 545, 554
DpnI GATC 2 cut(s) 174, 432
DpnII GATC 2 cut(s) 172, 430
DriI GACNNNNNGTC 2 cut(s) 84, 339
Eam1104I CTCTTC 3 cut(s) 69, 383, 602
Eam1105I GACNNNNNGTC 2 cut(s) 84, 339
EarI CTCTTC 3 cut(s) 69, 383, 602
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 5
Eco53kI GAGCTC 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 521
EcoICRI GAGCTC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 2 cut(s) 320, 495
FaqI GGGAC 4 cut(s) 104, 346, 485, 513
FatI CATG 2 cut(s) 316, 491
Fnu4HI GCNGC 1 cut(s) 674
FokI GGATG 2 cut(s) 682, 693
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 674
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 674
HaeIII GGCC 2 cut(s) 413, 520
HapII CCGG 2 cut(s) 81, 220
HgaI GACGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 2 cut(s) 320, 495
HincII GTYRAC 1 cut(s) 624
HindII GTYRAC 1 cut(s) 624
HinfI GANTC 4 cut(s) 155, 541, 550, 693
HpaII CCGG 2 cut(s) 81, 220
HphI GGTGA 2 cut(s) 217, 265
Hpy166II GTNNAC 4 cut(s) 5, 344, 392, 624
Hpy188I TCNGA 2 cut(s) 15, 164
Hpy188III TCNNGA 4 cut(s) 302, 425, 547, 556
Hpy8I GTNNAC 4 cut(s) 5, 344, 392, 624
Hpy99I CGWCG 1 cut(s) 90
HpyCH4III ACNGT 3 cut(s) 247, 341, 396
HpyCH4V TGCA 5 cut(s) 107, 143, 217, 392, 676
HpyF10VI GCNNNNNNNGC 1 cut(s) 583
HpyF3I CTNAG 3 cut(s) 12, 545, 554
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 2 cut(s) 320, 495
Kzo9I GATC 2 cut(s) 172, 430
Lsp1109I GCAGC 1 cut(s) 660
LweI GCATC 1 cut(s) 660
MabI ACCWGGT 1 cut(s) 46
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 174, 432
MboI GATC 2 cut(s) 172, 430
MboII GAAGA 6 cut(s) 86, 370, 440, 619, 668, 702
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 198, 394
MluCI AATT 4 cut(s) 30, 208, 294, 648
MlyI GAGTC 1 cut(s) 544
MnlI CCTC 9 cut(s) 18, 21, 163, 364, 403, 432, 432, 462, 490
MseI TTAA 6 cut(s) 177, 224, 288, 297, 617, 727
MslI CAYNNNNRTG 3 cut(s) 148, 203, 387
MspI CCGG 2 cut(s) 81, 220
MspR9I CCNGG 2 cut(s) 48, 82
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 1 cut(s) 361
MwoI GCNNNNNNNGC 1 cut(s) 583
NciI CCSGG 1 cut(s) 82
NdeII GATC 2 cut(s) 172, 430
NlaIII CATG 2 cut(s) 320, 495
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 495
NspV TTCGAA 1 cut(s) 450
PaeI GCATGC 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 562
PfeI GAWTC 3 cut(s) 155, 541, 693
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 1 cut(s) 675
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 2 cut(s) 5, 519
PstI CTGCAG 1 cut(s) 678
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 2 cut(s) 131, 276
RsaNI GTAC 2 cut(s) 130, 275
RseI CAYNNNNRTG 3 cut(s) 148, 203, 387
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 6 cut(s) 177, 224, 288, 297, 617, 727
SatI GCNGC 1 cut(s) 674
Sau3AI GATC 2 cut(s) 172, 430
Sau96I GGNCC 2 cut(s) 5, 519
ScaI AGTACT 1 cut(s) 276
SchI GAGTC 1 cut(s) 544
ScrFI CCNGG 2 cut(s) 48, 82
SduI GDGCHC 2 cut(s) 198, 394
SexAI ACCWGGT 1 cut(s) 46
SfaNI GCATC 1 cut(s) 660
SfcI CTRYAG 2 cut(s) 60, 674
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 3 cut(s) 148, 203, 387
SphI GCATGC 1 cut(s) 495
Sse9I AATT 4 cut(s) 30, 208, 294, 648
SsiI CCGC 1 cut(s) 361
SspI AATATT 1 cut(s) 181
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 46, 80
TaaI ACNGT 3 cut(s) 247, 341, 396
TaqI TCGA 2 cut(s) 55, 450
TasI AATT 4 cut(s) 30, 208, 294, 648
TatI WGTACW 2 cut(s) 129, 274
TfiI GAWTC 3 cut(s) 155, 541, 693
Tru1I TTAA 6 cut(s) 177, 224, 288, 297, 617, 727
Tru9I TTAA 6 cut(s) 177, 224, 288, 297, 617, 727
TscAI CASTG 2 cut(s) 252, 399
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 1 cut(s) 673
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 305, 614, 669
TspRI CASTG 2 cut(s) 252, 399
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 5
VspI ATTAAT 1 cut(s) 297
XceI RCATGY 1 cut(s) 495
XspI CTAG 1 cut(s) 92
ZrmI AGTACT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.