MD03G1089200.v1.1

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000175
Physical Location & Seq
Reverse (-)
7340949 .. 7345605
4657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1089200.v1.1.491

Sequence Viewer

Length: 3831 bp
ATGGAAGGATTTGAATTCGGCGATGGCTTGAATTTAGTGTATTCTGATGAGAATCTTGCAGATGGGTTCGTAGAGAATCATGAATCCACCAACCCAAATTTCTTTCCATCTAACTTAGATCATCCTAGTGATTTAAGCACTTCTTTGAGCCCGGGTTCGGATGCTGTTACTATCGACACTAATGACTGCAATCAACCTGTGCTCAAGTACATAAGTGAAATTCTTCTGGAGGAAGATTTGGAGGGTAAACCCTGCATGTTGCAGGAATCTTTGGCCCTCCAAGTTGCTGAAAAATATTTTTATGATGCCCTTAATCCGGAGGACCCTCCTTTACTCAACCAACCCTCTTTCTCTGTGTGCCAAAGCTTTGAGAACTCGGATGTTGGTTGTCATAGCAGTGATGGATCTATTGCCAGTTTTCCTAGCAGTAATGGCTCTATTTCTGCTAATAGTGGCTGGGTCTGGGATTTTTCACAGCCCTCGCATGGAATCAAGTTCCTTCCAAATGAAAGTTGTGAAACCATTGACCTTGAGAGGGACCAGTTAGCGCCTCCATGTTCTGATCAGTGGCCTCCAGGTCCTTACACGCTGATTGGGAATTTGGCATCGGAGAATGATGGCTACAGCTCAATAAATGGACCAAAGGGAAAGAAGAATCATCGAAGGGAGGATGGCTATGATGCAGAAGAAGGAAGAAGAAACAAGCAGTCAGCTGCTGCTTCTGACAATTCTGATCTGCAAGAAATATATGATAAGGTACTACAGAGTGTGAACCATGAACCTGACTCGTGTTTGCATGATGAATCTTCAAAAACTAAGGGAAATAGGACGTTGCAGCATAAGGAGCAGTCAAAAGGATGTAAATCATCACGTGCAAAGAAACAAAACAGCAACCCGAAAGTAGTAGACTTGTGCAAACTGCTAACTGAATGCGCAGAAGCTGTGGCAAGCTATGACCAACAAGCTGCAAATGAACTACTCAAGCAGATAAGGCAGCACTCTTCGCCCTATGGTGATTCAACCCAGCGATTAGCTCATTACTTCGCTGATGGCTTAGAGGCACGGTTGGCTGGTGCCAGAACCCCGTCATATTGCCCTCTTACGACGATGCACTGTCAAGCTACCGAAATCTTAAAGGCTTACCAGGTGTATGTTACTGCATGCCCTTTCAAGAAGATGGTGCACTTCTTTGCAAACAGAACAATTATGAAAACAGCAGAGAAGGCGACAAGGCTTCACATTATTGATTTTGGTAGTTCTTATGGTTTGCAATGGCCCTGCCTTATCCAGAGTCTCTCAGAGAAAACTGGCGGGCCTCCTAATCTCCGTATCACATTAATTGAGCTTCCCCAACCAGGTTTTCGACCTGCAGAAAGAATTGAAGAGACGGCGCGTCGCTTGACTAAATACTGTGAGAGGTATAATGTCCCATTTGAGTACAACGTCCTTGCCAAGAAATGGGAAACCATCAGACTGGAGGATTTTCGTATTGACAGAAATGAGGTGACTGTGGTTAACTGTATGCACCGGTTGAAGCACATGCCTGATGAAACAGTGATGGCGAACAATCCAAGAGATGCTGTCTTAAATTTGATCAAGAGTCTCAATCCAGACCTTTTTGTGCACGGTGTTGTCAATGGAACATACAATGCACCCGCCTTTGCCACACGGTTCAAACAGGCACTGTTCCACTTCTATACGTTCTTTGATATGTATGAGGCCACTGTTCCGCACGAAGATGAACAAAGAATGTTCTTCGAAAGAGCAATATTTGGGAGGGACATAATGAATGTCGTAGCGTGCGAGGGACTGGAGAGAGTTGAAAGGCCTGAGACATACAAGCAGTGGCAGACTAGGTATGCGAGGATCGGGTTCAATCAGCTCCCGTTAGACCGGGAGCTCTTGAAGAAAGTGAAGACCATGTCGAAGGTGATGGGGTATCATCGTGATTTCCGAATCGAAGATGATGGCCAGTGGATGCTGCAGGGATGGAAAGGAAGAATCAATTGTTCTACTCGAAATCTAGTAAATCATGATTCAAGCTCTTCCCAAAATCATGTAAATGGATTCCAAGTAAACCATGAACACACCAATCCAATTTTCCTTCCACCCATCTCAGACCATCCAAGTGATTCCGTGTTTTCGAGCCCTGGTTCGGATGCAGATACTATTGACATTAGTGATTGCAATCATCCTGTGCTCAAGTACATAAGTGATATTCTTTTGGAAGAAGATTTGGAGGGTAAGCCCTGCATGTTGCAGGACTGCTTGACCCTCCAAGCTGCTGAGAAATCTTTCTATGATGTTCTCAATCCAAAGGGCCCTCCTTCGTCCAGTCAATCCCCTCTTCCGATGTACCAAAGCTTTGAAAACTCACATGATGATTTCACACAGTGTTGTCATAGTAGTAATGGCCGTGTTGCTGCTAGGACACACTCGGTATCGAGTTCAGAAGCCTCTCATGTCCAATCTTGTCCGAAGGGAGAAAAAAAACAGTCAGCTGCCTCTAATGATGGCCCTGAGCCACAAGAATTGTTTGATGAGGTTTTGCTCTGTCAGGATGCGGACCACGAGTTTAAGTCTTGTTTTCACGATCAATATGTCAAAACTGATGCAAGTGGGAAGTTGCAGTGCAACAAGCAACCGAAAGTTTCTAAAGCAGCACGCTCAAAGACAAAGCATAAGAACAGGGAAGTAGTAGATTTGTGCACAATGCTGACTCAATGCGCACAAGCTGTGGCAAACCATGACCAGCGAACTGCGAGTGAACTAATCAAGAAGATAGGGCAGCACTCTTCTCCCAATGGTGATGCAACCGAGAGATTAGCTTATTACTTTGCTGACGCCCTAGAGGTACGCTTGGCTGGCTCCAGCACCCCCTCGTATTCTCCTCTTTTAAGTACACAGACACCAGCTACCGAAATCTTAAAAGCTCACCAGGTGTATGTTACTTCATGCCCTTTCAAGAAGATGTTGTACTTCTTCGCCAATAGAACAGTTATCAAACTAGCAGAAAATGCTACGAGGCTTCACATAATTGATTTTGGTATTTCTTATGGTTTCCAATGGTCATGCCTTATCCAACGCCTCTCTAAGAGACCTGGTGGACCTCCTAACCTTCGTATCACAGCAATTGAGCTCCCCCAACCAGGTTTTCGACCCACTGAAAGGGTTGAAGAGACAATGCATCGCCTAGAAAAATATGCTGAGAGATTTGATGTCCCATTTGAGTATACTGTGATTGCTCAGAAATGGGAAACTATCCGATTTGAGGATCTTAAAGTTGACAAATATGAGATGATTGTTGTTAACTGTATGCGCCGGTTAAGGCACATACCCGATGAAACAGTCGCGGTGAGCAGTCCAAGAGACACTGTCCTAAACTTGATCGAGAAAATAAATCCAGACCTTTTCATTCACGGAGTTGTCAATGGGACATACAATTCCCCCTTCTTTGTCACGCGGTTCAGAGAGGCGTTCTTCCACTACTCTGCGTTGTCTGATACGTTTGAGGCTACTATTCCTCGCGAAGATGAACATCGTCTAATGTTCGAAAAAGCAATATATGGTAGAGATATAATGAATGTCATAGCGTGCGAGGGACTAGAACGAGTTGAAAGGCCGGAGACATACAAGTATTGGCAGGTTAGGTATCACCGGGCGGGGTTCAAGCAGGTGCCCCTGGACCAGGAGCTCTTGAAGAAAGTGAAAGCCATGCTGAAGTTGATGCGTTATCATAACGATTTTCGAATAGATGAAGATGGCCACTGGATGCTGCAAGGATGGAAAGGAAGAATCATCATTGCTCTTTCTGCTTTGAAGCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

1277

Amino Acids

144.94

Weight (kDa)

5.93

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 306 - 667 3.2e-116 GRAS domain family
GRAS PF03514 904 - 1272 1.3e-116 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3664
Acc16I TGCGCA 2 cut(s) 934, 2728
Acc36I ACCTGC 3 cut(s) 1375, 3634, 3664
AccB1I GGYRCC 2 cut(s) 1073, 3676
AccB7I CCANNNNNTGG 1 cut(s) 1458
AccI GTMKAC 2 cut(s) 906, 3233
AccII CGCG 4 cut(s) 1393, 3353, 3463, 3528
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 7 cut(s) 1311, 1656, 1730, 2564, 3353, 3463, 3662
AclWI GGATC 3 cut(s) 412, 1874, 3282
AcoI YGGCCR 3 cut(s) 1969, 2413, 3763
AcsI RAATTY 6 cut(s) 14, 31, 97, 219, 598, 1588
AcuI CTGAAG 1 cut(s) 3740
AcvI CACGTG 1 cut(s) 872
AcyI GRCGYC 1 cut(s) 2844
AdeI CACNNNGTG 2 cut(s) 2394, 2941
AfaI GTAC 8 cut(s) 209, 759, 1439, 2207, 2357, 2856, 2902, 2978
AgeI ACCGGT 1 cut(s) 1527
AhdI GACNNNNNGTC 1 cut(s) 1392
AjnI CCWGG 9 cut(s) 574, 1143, 1354, 2149, 2937, 3100, 3148, 3681, 3687
AjuI GAANNNNNNNTTGG 8 cut(s) 83, 115, 1344, 1376, 2084, 2116, 3138, 3170
Alw21I GWGCWC 8 cut(s) 204, 1185, 1626, 1902, 2202, 2711, 3141, 3696
Alw26I GTCTC 8 cut(s) 1298, 1379, 1607, 1826, 3091, 3173, 3363, 3620
Alw44I GTGCAC 3 cut(s) 1181, 1622, 2707
AlwI GGATC 3 cut(s) 412, 1874, 3282
AlwNI CAGNNNCTG 3 cut(s) 716, 941, 1684
Ama87I CYCGRG 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 316
ApaI GGGCCC 1 cut(s) 2324
ApaLI GTGCAC 3 cut(s) 1181, 1622, 2707
ApoI RAATTY 6 cut(s) 14, 31, 97, 219, 598, 1588
AseI ATTAAT 1 cut(s) 1337
AsiGI ACCGGT 1 cut(s) 1527
Asp700I GAANNNNTTC 2 cut(s) 222, 2294
AspLEI GCGC 5 cut(s) 550, 935, 1393, 2729, 3321
AsuC2I CCSGG 4 cut(s) 152, 153, 1895, 3659
AsuHPI GGTGA 7 cut(s) 1025, 1516, 1942, 2819, 2927, 3367, 3647
AsuII TTCGAA 3 cut(s) 1758, 3552, 3748
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 7 cut(s) 322, 538, 578, 638, 2566, 3107, 3685
BaeGI GKGCMC 5 cut(s) 1185, 1626, 2324, 2711, 3681
BaeI ACNNNNGTAYC 2 cut(s) 3106, 3139
BalI TGGCCA 2 cut(s) 1971, 3765
BanI GGYRCC 2 cut(s) 1073, 3676
BanII GRGCYC 6 cut(s) 152, 1902, 2150, 2324, 3141, 3696
BarI GAAGNNNNNNTAC 2 cut(s) 2960, 2992
BauI CACGAG 2 cut(s) 787, 2570
BbrPI CACGTG 1 cut(s) 872
BbsI GAAGAC 1 cut(s) 1922
Bbv12I GWGCWC 8 cut(s) 204, 1185, 1626, 1902, 2202, 2711, 3141, 3696
BceAI ACGGC 2 cut(s) 1404, 2400
BciT130I CCWGG 9 cut(s) 576, 1145, 1356, 2151, 2939, 3102, 3150, 3683, 3689
BclI TGATCA 2 cut(s) 562, 1593
BcnI CCSGG 4 cut(s) 152, 153, 1895, 3659
BcoDI GTCTC 8 cut(s) 1298, 1379, 1607, 1826, 3091, 3173, 3363, 3620
BfaI CTAG 9 cut(s) 126, 423, 1854, 2024, 2427, 2849, 3008, 3194, 3605
BfmI CTRYAG 4 cut(s) 622, 761, 1368, 1982
BfoI RGCGCY 1 cut(s) 551
BfuAI ACCTGC 3 cut(s) 1375, 3634, 3664
Bme18I GGWCC 7 cut(s) 322, 538, 578, 638, 2566, 3107, 3685
BmeRI GACNNNNNGTC 1 cut(s) 1392
BmeT110I CYCGRG 1 cut(s) 151
BmiI GGNNCC 6 cut(s) 324, 539, 1075, 2322, 2869, 3678
BpiI GAAGAC 1 cut(s) 1922
BpmI CTGGAG 5 cut(s) 248, 558, 1496, 1832, 2854
Bpu10I CCTNAGC 1 cut(s) 2520
Bpu14I TTCGAA 3 cut(s) 1758, 3552, 3748
BpuEI CTTGAG 4 cut(s) 188, 551, 965, 2186
BpuMI CCSGG 4 cut(s) 152, 153, 1895, 3659
BsaAI YACGTR 1 cut(s) 872
BsaBI GATNNNNATC 4 cut(s) 51, 862, 2187, 3537
BsaHI GRCGYC 1 cut(s) 2844
BsaI GGTCTC 1 cut(s) 3091
BsaJI CCNNGG 3 cut(s) 151, 2149, 3681
BsaWI WCCGGW 2 cut(s) 316, 1527
Bse118I RCCGGY 2 cut(s) 1527, 3321
Bse1I ACTGG 8 cut(s) 414, 541, 1312, 1479, 1815, 1972, 2334, 3773
Bse3DI GCAATG 2 cut(s) 1277, 3801
Bse8I GATNNNNATC 4 cut(s) 51, 862, 2187, 3537
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 9 cut(s) 576, 1145, 1356, 2151, 2939, 3102, 3150, 3683, 3689
BseDI CCNNGG 3 cut(s) 151, 2149, 3681
BseJI GATNNNNATC 4 cut(s) 51, 862, 2187, 3537
BseMI GCAATG 2 cut(s) 1277, 3801
BseMII CTCAG 7 cut(s) 1311, 1821, 2130, 2277, 2511, 3198, 3260
BseNI ACTGG 8 cut(s) 414, 541, 1312, 1479, 1815, 1972, 2334, 3773
BseRI GAGGAG 1 cut(s) 2880
BseSI GKGCMC 5 cut(s) 1185, 1626, 2324, 2711, 3681
BseYI CCCAGC 2 cut(s) 456, 1023
Bsh1236I CGCG 4 cut(s) 1393, 3353, 3463, 3528
BshNI GGYRCC 2 cut(s) 1073, 3676
BshTI ACCGGT 1 cut(s) 1527
BsiHKAI GWGCWC 8 cut(s) 204, 1185, 1626, 1902, 2202, 2711, 3141, 3696
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 7 cut(s) 152, 317, 1528, 1894, 3322, 3623, 3658
BslFI GGGAC 7 cut(s) 551, 1412, 1793, 1821, 3206, 3448, 3615
BsmAI GTCTC 8 cut(s) 1298, 1379, 1607, 1826, 3091, 3173, 3363, 3620
BsmBI CGTCTC 1 cut(s) 1379
BsmFI GGGAC 7 cut(s) 551, 1412, 1793, 1821, 3206, 3448, 3615
BsmI GAATGC 1 cut(s) 935
Bso31I GGTCTC 1 cut(s) 3091
BsoBI CYCGRG 1 cut(s) 151
Bsp119I TTCGAA 3 cut(s) 1758, 3552, 3748
Bsp120I GGGCCC 1 cut(s) 2320
Bsp13I TCCGGA 1 cut(s) 316
Bsp143I GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
Bsp68I TCGCGA 1 cut(s) 3528
BspACI CCGC 7 cut(s) 1311, 1656, 1730, 2564, 3353, 3463, 3662
BspCNI CTCAG 7 cut(s) 1310, 1822, 2129, 2278, 2512, 3199, 3259
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 4 cut(s) 1393, 3353, 3463, 3528
BspHI TCATGA 2 cut(s) 79, 2032
BspLI GGNNCC 6 cut(s) 324, 539, 1075, 2322, 2869, 3678
BspMAI CTGCAG 2 cut(s) 1372, 1986
BspMI ACCTGC 3 cut(s) 1375, 3634, 3664
BspPI GGATC 3 cut(s) 412, 1874, 3282
BspQI GCTCTTC 1 cut(s) 2050
BspT104I TTCGAA 3 cut(s) 1758, 3552, 3748
BspT107I GGYRCC 2 cut(s) 1073, 3676
BspTNI GGTCTC 1 cut(s) 3091
BsrDI GCAATG 2 cut(s) 1277, 3801
BsrFI RCCGGY 2 cut(s) 1527, 3321
BsrI ACTGG 8 cut(s) 414, 541, 1312, 1479, 1815, 1972, 2334, 3773
BssAI RCCGGY 2 cut(s) 1527, 3321
BssECI CCNNGG 3 cut(s) 151, 2149, 3681
BssMI GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
BssNAI GTATAC 1 cut(s) 3234
BssNI GRCGYC 1 cut(s) 2844
BssSI CACGAG 2 cut(s) 787, 2570
Bst1107I GTATAC 1 cut(s) 3234
Bst2BI CACGAG 2 cut(s) 787, 2570
Bst2UI CCWGG 9 cut(s) 576, 1145, 1356, 2151, 2939, 3102, 3150, 3683, 3689
Bst6I CTCTTC 6 cut(s) 1006, 1377, 2050, 2352, 2800, 3171
BstACI GRCGYC 1 cut(s) 2844
BstAPI GCANNNNNTGC 1 cut(s) 3017
BstBAI YACGTR 1 cut(s) 872
BstBI TTCGAA 3 cut(s) 1758, 3552, 3748
BstC8I GCNNGC 8 cut(s) 949, 1162, 1313, 1801, 2665, 2866, 3595, 3825
BstENI CCTNNNNNAGG 1 cut(s) 3686
BstFNI CGCG 4 cut(s) 1393, 3353, 3463, 3528
BstH2I RGCGCY 1 cut(s) 551
BstHHI GCGC 5 cut(s) 550, 935, 1393, 2729, 3321
BstKTI GATC 9 cut(s) 121, 407, 565, 736, 1596, 1869, 2596, 3277, 3390
BstMAI GTCTC 8 cut(s) 1298, 1379, 1607, 1826, 3091, 3173, 3363, 3620
BstMBI GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
BstMWI GCNNNNNNNGC 9 cut(s) 432, 844, 991, 1003, 1067, 1223, 2865, 3017, 3812
BstNI CCWGG 9 cut(s) 576, 1145, 1356, 2151, 2939, 3102, 3150, 3683, 3689
BstNSI RCATGY 4 cut(s) 259, 1164, 1543, 2257
BstSFI CTRYAG 4 cut(s) 622, 761, 1368, 1982
BstSLI GKGCMC 5 cut(s) 1185, 1626, 2324, 2711, 3681
BstUI CGCG 4 cut(s) 1393, 3353, 3463, 3528
BstV2I GAAGAC 1 cut(s) 1922
BstX2I RGATCY 2 cut(s) 404, 3274
BstXI CCANNNNNNTGG 1 cut(s) 1474
BstYI RGATCY 2 cut(s) 404, 3274
BstZ17I GTATAC 1 cut(s) 3234
BtgZI GCGATG 2 cut(s) 36, 3173
BtsI GCAGTG 3 cut(s) 403, 1850, 2636
BtuMI TCGCGA 1 cut(s) 3528
BveI ACCTGC 3 cut(s) 1375, 3634, 3664
Cac8I GCNNGC 8 cut(s) 949, 1162, 1313, 1801, 2665, 2866, 3595, 3825
CaiI CAGNNNCTG 3 cut(s) 716, 941, 1684
CciI TCATGA 2 cut(s) 79, 2032
CfoI GCGC 5 cut(s) 550, 935, 1393, 2729, 3321
Cfr10I RCCGGY 2 cut(s) 1527, 3321
Cfr9I CCCGGG 1 cut(s) 151
CseI GACGC 2 cut(s) 1382, 2852
CsiI ACCWGGT 5 cut(s) 1143, 1354, 2937, 3100, 3148
Csp6I GTAC 8 cut(s) 208, 758, 1438, 2206, 2356, 2855, 2901, 2977
CspAI ACCGGT 1 cut(s) 1527
CspCI CAANNNNNGTGG 2 cut(s) 2514, 2549
CviQI GTAC 8 cut(s) 208, 758, 1438, 2206, 2356, 2855, 2901, 2977
DpnI GATC 9 cut(s) 120, 406, 564, 735, 1595, 1868, 2595, 3276, 3389
DpnII GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
DraIII CACNNNGTG 2 cut(s) 2394, 2941
DriI GACNNNNNGTC 1 cut(s) 1392
EaeI YGGCCR 3 cut(s) 1969, 2413, 3763
Eam1104I CTCTTC 6 cut(s) 1006, 1377, 2050, 2352, 2800, 3171
Eam1105I GACNNNNNGTC 1 cut(s) 1392
EarI CTCTTC 6 cut(s) 1006, 1377, 2050, 2352, 2800, 3171
Ecl136II GAGCTC 3 cut(s) 1900, 3139, 3694
Eco147I AGGCCT 1 cut(s) 1828
Eco24I GRGCYC 6 cut(s) 152, 1902, 2150, 2324, 3141, 3696
Eco31I GGTCTC 1 cut(s) 3091
Eco47I GGWCC 7 cut(s) 322, 538, 578, 638, 2566, 3107, 3685
Eco53kI GAGCTC 3 cut(s) 1900, 3139, 3694
Eco57I CTGAAG 1 cut(s) 3740
Eco72I CACGTG 1 cut(s) 872
Eco88I CYCGRG 1 cut(s) 151
EcoICRI GAGCTC 3 cut(s) 1900, 3139, 3694
EcoNI CCTNNNNNAGG 1 cut(s) 3686
EcoO109I RGGNCCY 4 cut(s) 322, 578, 2320, 2321
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 9 cut(s) 574, 1143, 1354, 2149, 2937, 3100, 3148, 3681, 3687
EcoT22I ATGCAT 1 cut(s) 3189
EcoT38I GRGCYC 6 cut(s) 152, 1902, 2150, 2324, 3141, 3696
Esp3I CGTCTC 1 cut(s) 1379
FalI AAGNNNNNCTT 2 cut(s) 127, 159
FaqI GGGAC 7 cut(s) 551, 1412, 1793, 1821, 3206, 3448, 3615
FauI CCCGC 3 cut(s) 1304, 1663, 3655
FbaI TGATCA 2 cut(s) 562, 1593
FblI GTMKAC 2 cut(s) 906, 3233
FriOI GRGCYC 6 cut(s) 152, 1902, 2150, 2324, 3141, 3696
FspAI RTGCGCAY 1 cut(s) 2728
FspBI CTAG 9 cut(s) 126, 423, 1854, 2024, 2427, 2849, 3008, 3194, 3605
FspI TGCGCA 2 cut(s) 934, 2728
GlaI GCGC 5 cut(s) 549, 934, 1392, 2728, 3320
GsaI CCCAGC 2 cut(s) 460, 1027
GsuI CTGGAG 5 cut(s) 248, 558, 1496, 1832, 2854
HaeII RGCGCY 1 cut(s) 551
HapII CCGG 7 cut(s) 152, 317, 1528, 1894, 3322, 3623, 3658
HgaI GACGC 2 cut(s) 1382, 2852
HhaI GCGC 5 cut(s) 550, 935, 1393, 2729, 3321
Hin1I GRCGYC 1 cut(s) 2844
Hin6I GCGC 5 cut(s) 548, 933, 1391, 2727, 3319
HinP1I GCGC 5 cut(s) 548, 933, 1391, 2727, 3319
HincII GTYRAC 3 cut(s) 1516, 3286, 3310
HindII GTYRAC 3 cut(s) 1516, 3286, 3310
HindIII AAGCTT 2 cut(s) 364, 2362
HpaI GTTAAC 2 cut(s) 1516, 3310
HpaII CCGG 7 cut(s) 152, 317, 1528, 1894, 3322, 3623, 3658
HphI GGTGA 7 cut(s) 1025, 1516, 1942, 2819, 2927, 3367, 3647
Hpy99I CGWCG 2 cut(s) 1108, 1398
HpyCH4IV ACGT 5 cut(s) 830, 871, 1443, 1700, 3506
HpyF10VI GCNNNNNNNGC 9 cut(s) 432, 844, 991, 1003, 1067, 1223, 2865, 3017, 3812
HpySE526I ACGT 5 cut(s) 830, 871, 1443, 1700, 3506
Hsp92I GRCGYC 1 cut(s) 2844
HspAI GCGC 5 cut(s) 548, 933, 1391, 2727, 3319
Kpn2I TCCGGA 1 cut(s) 316
Ksp22I TGATCA 2 cut(s) 562, 1593
KspAI GTTAAC 2 cut(s) 1516, 3310
Kzo9I GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
LguI GCTCTTC 1 cut(s) 2050
LmnI GCTCC 6 cut(s) 844, 1887, 1897, 2873, 3144, 3691
MabI ACCWGGT 5 cut(s) 1143, 1354, 2937, 3100, 3148
MaeI CTAG 9 cut(s) 126, 423, 1854, 2024, 2427, 2849, 3008, 3194, 3605
MaeII ACGT 5 cut(s) 830, 871, 1443, 1700, 3506
MaeIII GTNAC 5 cut(s) 166, 1153, 1504, 2947, 3457
MalI GATC 9 cut(s) 120, 406, 564, 735, 1595, 1868, 2595, 3276, 3389
MboI GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
MfeI CAATTG 2 cut(s) 2005, 3132
MflI RGATCY 2 cut(s) 404, 3274
MlsI TGGCCA 2 cut(s) 1971, 3765
MluNI TGGCCA 2 cut(s) 1971, 3765
MlyI GAGTC 4 cut(s) 779, 1300, 1609, 2713
MmeI TCCRAC 1 cut(s) 3106
Mox20I TGGCCA 2 cut(s) 1971, 3765
Mph1103I ATGCAT 1 cut(s) 3189
MroI TCCGGA 1 cut(s) 316
MroXI GAANNNNTTC 2 cut(s) 222, 2294
MscI TGGCCA 2 cut(s) 1971, 3765
MslI CAYNNNNRTG 5 cut(s) 126, 396, 1737, 2061, 2127
Msp20I TGGCCA 2 cut(s) 1971, 3765
MspA1I CMGCKG 2 cut(s) 713, 2501
MspI CCGG 7 cut(s) 152, 317, 1528, 1894, 3322, 3623, 3658
MunI CAATTG 2 cut(s) 2005, 3132
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 9 cut(s) 576, 1145, 1356, 2151, 2939, 3102, 3150, 3683, 3689
MvnI CGCG 4 cut(s) 1393, 3353, 3463, 3528
MwoI GCNNNNNNNGC 9 cut(s) 432, 844, 991, 1003, 1067, 1223, 2865, 3017, 3812
NciI CCSGG 4 cut(s) 152, 153, 1895, 3659
NdeII GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
NlaIV GGNNCC 6 cut(s) 324, 539, 1075, 2322, 2869, 3678
NmuCI GTSAC 2 cut(s) 1504, 3457
NruI TCGCGA 1 cut(s) 3528
NsbI TGCGCA 2 cut(s) 934, 2728
NsiI ATGCAT 1 cut(s) 3189
NspI RCATGY 4 cut(s) 259, 1164, 1543, 2257
NspV TTCGAA 3 cut(s) 1758, 3552, 3748
PaeI GCATGC 1 cut(s) 1164
PagI TCATGA 2 cut(s) 79, 2032
PaqCI CACCTGC 1 cut(s) 3664
PceI AGGCCT 1 cut(s) 1828
PciSI GCTCTTC 1 cut(s) 2050
PcsI WCGNNNNNNNCGW 2 cut(s) 1800, 1951
PctI GAATGC 1 cut(s) 935
PdmI GAANNNNTTC 2 cut(s) 222, 2294
PflFI GACNNNGTC 2 cut(s) 1921, 3374
PflMI CCANNNNNTGG 1 cut(s) 1458
PinAI ACCGGT 1 cut(s) 1527
PleI GAGTC 4 cut(s) 779, 1299, 1608, 2713
PmaCI CACGTG 1 cut(s) 872
PmlI CACGTG 1 cut(s) 872
PpsI GAGTC 4 cut(s) 779, 1299, 1608, 2713
Ppu21I YACGTR 1 cut(s) 872
PpuMI RGGWCCY 2 cut(s) 322, 578
PshBI ATTAAT 1 cut(s) 1337
Psp124BI GAGCTC 3 cut(s) 1902, 3141, 3696
Psp5II RGGWCCY 2 cut(s) 322, 578
Psp6I CCWGG 9 cut(s) 574, 1143, 1354, 2149, 2937, 3100, 3148, 3681, 3687
PspCI CACGTG 1 cut(s) 872
PspFI CCCAGC 2 cut(s) 456, 1023
PspGI CCWGG 9 cut(s) 574, 1143, 1354, 2149, 2937, 3100, 3148, 3681, 3687
PspN4I GGNNCC 6 cut(s) 324, 539, 1075, 2322, 2869, 3678
PspOMI GGGCCC 1 cut(s) 2320
PspPPI RGGWCCY 2 cut(s) 322, 578
PstI CTGCAG 2 cut(s) 1372, 1986
PstNI CAGNNNCTG 3 cut(s) 716, 941, 1684
PsuI RGATCY 2 cut(s) 404, 3274
PsyI GACNNNGTC 2 cut(s) 1921, 3374
PvuII CAGCTG 2 cut(s) 713, 2501
RruI TCGCGA 1 cut(s) 3528
RsaI GTAC 8 cut(s) 209, 759, 1439, 2207, 2357, 2856, 2902, 2978
RsaNI GTAC 8 cut(s) 208, 758, 1438, 2206, 2356, 2855, 2901, 2977
RseI CAYNNNNRTG 5 cut(s) 126, 396, 1737, 2061, 2127
SacI GAGCTC 3 cut(s) 1902, 3141, 3696
SapI GCTCTTC 1 cut(s) 2050
Sau3AI GATC 9 cut(s) 118, 404, 562, 733, 1593, 1866, 2593, 3274, 3387
SchI GAGTC 4 cut(s) 779, 1300, 1609, 2713
SexAI ACCWGGT 5 cut(s) 1143, 1354, 2937, 3100, 3148
SfcI CTRYAG 4 cut(s) 622, 761, 1368, 1982
SfuI TTCGAA 3 cut(s) 1758, 3552, 3748
SinI GGWCC 7 cut(s) 322, 538, 578, 638, 2566, 3107, 3685
SmaI CCCGGG 1 cut(s) 153
SmiMI CAYNNNNRTG 5 cut(s) 126, 396, 1737, 2061, 2127
SmlI CTYRAG 4 cut(s) 203, 530, 980, 2201
SmoI CTYRAG 4 cut(s) 203, 530, 980, 2201
SphI GCATGC 1 cut(s) 1164
SseBI AGGCCT 1 cut(s) 1828
SsiI CCGC 7 cut(s) 1311, 1656, 1730, 2564, 3353, 3463, 3662
SspI AATATT 2 cut(s) 296, 1770
SspMI CTAG 9 cut(s) 126, 423, 1854, 2024, 2427, 2849, 3008, 3194, 3605
SstI GAGCTC 3 cut(s) 1902, 3141, 3696
StuI AGGCCT 1 cut(s) 1828
TaiI ACGT 5 cut(s) 833, 874, 1446, 1703, 3509
TatI WGTACW 5 cut(s) 207, 1437, 2205, 2900, 2976
TseFI GTSAC 2 cut(s) 1504, 3457
Tsp45I GTSAC 2 cut(s) 1504, 3457
TspGWI ACGGA 3 cut(s) 1316, 2125, 3435
TspMI CCCGGG 1 cut(s) 151
Tth111I GACNNNGTC 2 cut(s) 1921, 3374
Van91I CCANNNNNTGG 1 cut(s) 1458
VneI GTGCAC 3 cut(s) 1181, 1622, 2707
VpaK11BI GGWCC 7 cut(s) 322, 538, 578, 638, 2566, 3107, 3685
VspI ATTAAT 1 cut(s) 1337
XagI CCTNNNNNAGG 1 cut(s) 3686
XapI RAATTY 6 cut(s) 14, 31, 97, 219, 598, 1588
XceI RCATGY 4 cut(s) 259, 1164, 1543, 2257
XmaI CCCGGG 1 cut(s) 151
XmiI GTMKAC 2 cut(s) 906, 3233
XmnI GAANNNNTTC 2 cut(s) 222, 2294
XspI CTAG 9 cut(s) 126, 423, 1854, 2024, 2427, 2849, 3008, 3194, 3605
Zsp2I ATGCAT 1 cut(s) 3189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.