Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000175

Physical Location & Seq

Genomic Coordinates Reverse (-)

7330830 .. 7332881

Gene Structure 2052 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1088900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGGATACCTTTGAGGATGAACTTCCCAGTCCCACGAACAAATCCGTAATCGAGCAAGTCGACGTCCCGGCTGACTTGAATGCGAATCCTGTTCATGAGTTTGAAGCCAACCATGATTTCACTGATCACAATTTCTTCCCTGTCAATTCAGACCCTTCAGATAATTTATCCTCGTTGCCGGGGGTGAGCACGGAGATGAATTTTCCGGAAAGCAGTGAAGCATCCAATGCAATTCTCAAGTACATAAGGGAGATGCTTATGGAGGAGGAGGAATACTTGGTGAACAAGCCCTGCATGCTGCAGGAAGGTTTGGCACTCCAAGCTGCCGAAAAGTCATTGCATGATGTGCTTGTTCAGGAGCAAAATCCCTCTTCAAGTGATCCACTTCTTAGTTCCATACATCGAAATGTTGAGGCCCCGAACGAGGGCAGTAATCACAGCAGTACTGGCTCTATAGCTGCTGGAAACCGGGTTGGCTCGGATTGGGGTTCTGTTCAAGATGTTTTCGAGTCCTCTCGTGCCCAAATTCCAAACCCTTTGGTTTTGAGCGGTGAGGGTGAAATCACGCATCTAGCACACAATTCATCGTCTGTTGTGGAGCTGAGGGAGCTGCAGGGAAAGGATGTGAGTTTCAATTCGGACAAGGATGGCAGCGACTCGTCAAATGGATCATGGAGTAAGAAGAGTCATCAGAGGGAGGACGAGGATTCTCTAGATGACTGGAGGAGAAACAAGGATCCAGCGGTTTATGCCGACGAATTTGAGCTACCGCAGCTGTTTGATGAGGTACTGCTCTGTCAGGGTGAGAAACAAGAGTCCAACTCATGTTCTCCCAAGGAAAGCGAGAAGTTGCAGCTCAACCGGAAGTCAAAAGGATCGAAGGGCAAGAAAACACGCAAGAAAAAGCTGGATGACAATGCGGGGATGGTTGATCTATGGACGCTGATGACTCTATGCGCACAAGCTGTTGCAAGCTATGACAAAAGGAATGCAAATGAACAACTCAAGCAGATAAGGCTGCACTCTTCTCCCTACGGCGATGGCACGCAGAGATTAGCTCATTACGTGGCAAATGGCCTCGAACTGCGCTTGGGTGCTGATGTTCCCTCGTACGCTTCTCTTCCCAGCAGTGAGATGTCAGCTGCTGATATCTTAAAAGCTTACCAGACTTACATCACGGCATGCCCTTTCCACAAGATGTCAAATATCTATGCTAACCAAACGATTCTGAAACAAGCAGAGAAGGGGACAAGGCTTCACATCATTGATTTCGGTGTCCTCTATGGCTACCAATGGCCTTCCCTCATCCAAGACCTCTCCAAGAGACACGGCGGACCTCCCATGCTTCGCATAACCGGAGTAGAGTTTCCCCAGCCAGGTTTCCGGCCTTCAGAAAGGGTTGAACAGACAGGGCGGCGCCTAGCAAATTACTGCAAGAGGTTTAACGTCCCCTTCAAGTACAACGTCATAGCAACGAATTGGGAAACAATTCGGTCTGAGGATATCAAAATCGACAGAGATGAGTTCACTGTAGTCAACTGCTTGTACCGGCTAAAAAATCTACGAGATGATACAGTGACAGACTGTCCTAGGGATACAGTTTTGAAGCTGATCCGAAGAATTAGCCCGGATTTGTTCATCCATGGGATTGTCAATGGATCCTACAATGCACCCTTCTTTGACATACGCTTCCGAGAAGCACTCTTCCATTTTTCTTCCCTGTTCAACATGTTCGAGGCGACTGTGCCACGAGAAGATCCACAGAGGTTGCTCTACGAGCAAGAAGTCTTTGGTAGAGATGTTATCAATGTGATAGCATGTGAGGGTCCAAGAAGGGTCGAAAGGCCAGAGACATACAAGCAGTGGCAGATTAGAAACAAGAGAGCCGGGTTCAGGCAGGTACCATCAGACCAGGAGATCTTGAAGGAAATCAGGAGTATGGTGAAGAGAGATTACCATAAGGATTTCTCCGTCGACGAAGATGGAATGTGGGTGTTGCAGGGATGGAAAGGCAGGATATTCCATGCTATTTCGTACTGGAAACCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

684

Amino Acids

77.7

Weight (kDa)

5.52

Isoelectric Point (pI)

53.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	315 - 681	1.9e-113	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca	FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica	MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica	Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis	pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis	RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata	RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora	Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii	Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa	Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha	Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana	Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	66
Acc16I	TGCGCA	1 cut(s)	958
Acc36I	ACCTGC	1 cut(s)	1888
Acc65I	GGTACC	1 cut(s)	1900
AccB1I	GGYRCC	2 cut(s)	1416, 1900
AccBSI	CCGCTC	1 cut(s)	549
AccI	GTMKAC	2 cut(s)	60, 1974
AccIII	TCCGGA	1 cut(s)	205
AciI	CCGC	6 cut(s)	549, 743, 770, 920, 1332, 1414
AclWI	GGATC	9 cut(s)	374, 676, 731, 744, 883, 1606, 1653, 1666, 1751
AcsI	RAATTY	3 cut(s)	199, 525, 758
AcuI	CTGAAG	2 cut(s)	141, 1374
AcyI	GRCGYC	2 cut(s)	63, 1417
AfaI	GTAC	8 cut(s)	242, 445, 789, 1112, 1460, 1547, 1902, 2036
AfiI	CCNNNNNNNGG	4 cut(s)	424, 425, 1376, 1893
AflIII	ACRYGT	1 cut(s)	1728
AhdI	GACNNNNNGTC	1 cut(s)	1584
AjnI	CCWGG	2 cut(s)	1375, 1911
AjuI	GAANNNNNNNTTGG	2 cut(s)	101, 133
Alw21I	GWGCWC	1 cut(s)	191
Alw26I	GTCTC	2 cut(s)	1318, 1844
AlwI	GGATC	9 cut(s)	374, 676, 731, 744, 883, 1606, 1653, 1666, 1751
AlwNI	CAGNNNCTG	1 cut(s)	1145
Aor13HI	TCCGGA	1 cut(s)	205
AoxI	GGCC	5 cut(s)	414, 1075, 1295, 1385, 1844
ApeKI	GCWGC	9 cut(s)	298, 323, 458, 610, 651, 772, 853, 1018, 1142
ApoI	RAATTY	3 cut(s)	199, 525, 758
Asp700I	GAANNNNTTC	1 cut(s)	1488
Asp718I	GGTACC	1 cut(s)	1900
AspA2I	CCTAGG	1 cut(s)	1589
AspLEI	GCGC	3 cut(s)	959, 1089, 1419
AspS9I	GGNCC	3 cut(s)	415, 1334, 1826
AsuC2I	CCSGG	5 cut(s)	68, 180, 470, 1628, 1888
AsuHPI	GGTGA	6 cut(s)	196, 292, 563, 569, 815, 1954
AvaII	GGWCC	2 cut(s)	1334, 1826
AvrII	CCTAGG	1 cut(s)	1589
BaeGI	GKGCMC	1 cut(s)	523
BamHI	GGATCC	2 cut(s)	736, 1658
BanI	GGYRCC	2 cut(s)	1416, 1900
BarI	GAAGNNNNNNTAC	2 cut(s)	1937, 1969
BauI	CACGAG	2 cut(s)	516, 1749
Bbv12I	GWGCWC	1 cut(s)	191
BbvCI	CCTCAGC	1 cut(s)	602
BbvI	GCAGC	9 cut(s)	285, 310, 445, 597, 663, 784, 865, 1005, 1129
BccI	CCATC	6 cut(s)	641, 919, 1034, 1912, 1976, 1998
BceAI	ACGGC	3 cut(s)	1051, 1194, 1345
BciT130I	CCWGG	2 cut(s)	1377, 1913
BciVI	GTATCC	1 cut(s)	1588
BclI	TGATCA	1 cut(s)	124
BcnI	CCSGG	5 cut(s)	68, 180, 470, 1628, 1888
BcoDI	GTCTC	2 cut(s)	1318, 1844
BfaI	CTAG	4 cut(s)	572, 713, 1421, 1590
BfmI	CTRYAG	4 cut(s)	299, 453, 611, 1530
BfoI	RGCGCY	1 cut(s)	1420
BfuAI	ACCTGC	1 cut(s)	1888
BfuI	GTATCC	1 cut(s)	1588
BglII	AGATCT	1 cut(s)	1917
BlnI	CCTAGG	1 cut(s)	1589
BmcAI	AGTACT	1 cut(s)	445
Bme1390I	CCNGG	7 cut(s)	68, 180, 470, 1377, 1628, 1888, 1913
Bme18I	GGWCC	2 cut(s)	1334, 1826
BmeRI	GACNNNNNGTC	1 cut(s)	1584
BmgT120I	GGNCC	3 cut(s)	415, 1334, 1826
BmiI	GGNNCC	6 cut(s)	417, 738, 1418, 1660, 1827, 1902
BmrFI	CCNGG	7 cut(s)	68, 180, 470, 1377, 1628, 1888, 1913
BmrI	ACTGGG	1 cut(s)	21
BmsI	GCATC	3 cut(s)	230, 243, 577
BmuI	ACTGGG	1 cut(s)	21
BplI	GAGNNNNNCTC	6 cut(s)	806, 838, 1042, 1074, 1686, 1718
BpmI	CTGGAG	1 cut(s)	742
Bpu10I	CCTNAGC	1 cut(s)	602
BpuEI	CTTGAG	2 cut(s)	221, 989
BpuMI	CCSGG	5 cut(s)	68, 180, 470, 1628, 1888
BsaAI	YACGTR	1 cut(s)	1066
BsaHI	GRCGYC	2 cut(s)	63, 1417
BsaJI	CCNNGG	4 cut(s)	179, 834, 1589, 1642
BsaWI	WCCGGW	3 cut(s)	205, 861, 1355
Bsc4I	CCNNNNNNNGG	4 cut(s)	424, 425, 1376, 1893
Bse118I	RCCGGY	1 cut(s)	1548
Bse1I	ACTGG	4 cut(s)	27, 451, 725, 2042
Bse3DI	GCAATG	1 cut(s)	335
BseAI	TCCGGA	1 cut(s)	205
BseBI	CCWGG	2 cut(s)	1377, 1913
BseDI	CCNNGG	4 cut(s)	179, 834, 1589, 1642
BseGI	GGATG	9 cut(s)	22, 221, 628, 652, 916, 930, 1305, 1638, 2009
BseLI	CCNNNNNNNGG	4 cut(s)	424, 425, 1376, 1893
BseMI	GCAATG	1 cut(s)	335
BseMII	CTCAG	2 cut(s)	593, 1488
BseNI	ACTGG	4 cut(s)	27, 451, 725, 2042
BseRI	GAGGAG	3 cut(s)	278, 281, 739
BseSI	GKGCMC	1 cut(s)	523
BseXI	GCAGC	9 cut(s)	285, 310, 445, 597, 663, 784, 865, 1005, 1129
BseYI	CCCAGC	2 cut(s)	1124, 1371
BsgI	GTGCAG	1 cut(s)	1004
BshFI	GGCC	5 cut(s)	416, 1077, 1297, 1387, 1846
BshNI	GGYRCC	2 cut(s)	1416, 1900
BsiHKAI	GWGCWC	1 cut(s)	191
BsiWI	CGTACG	1 cut(s)	1110
BslFI	GGGAC	4 cut(s)	15, 50, 1261, 1433
BslI	CCNNNNNNNGG	4 cut(s)	424, 425, 1376, 1893
BsmAI	GTCTC	2 cut(s)	1318, 1844
BsmFI	GGGAC	4 cut(s)	15, 50, 1261, 1433
BsmI	GAATGC	2 cut(s)	85, 994
BsnI	GGCC	5 cut(s)	416, 1077, 1297, 1387, 1846
Bsp1286I	GDGCHC	2 cut(s)	191, 523
Bsp13I	TCCGGA	1 cut(s)	205
Bsp19I	CCATGG	1 cut(s)	1642
BspACI	CCGC	6 cut(s)	549, 743, 770, 920, 1332, 1414
BspANI	GGCC	5 cut(s)	416, 1077, 1297, 1387, 1846
BspCNI	CTCAG	2 cut(s)	594, 1489
BspEI	TCCGGA	1 cut(s)	205
BspHI	TCATGA	1 cut(s)	94
BspLI	GGNNCC	6 cut(s)	417, 738, 1418, 1660, 1827, 1902
BspMAI	CTGCAG	2 cut(s)	303, 615
BspMI	ACCTGC	1 cut(s)	1888
BspPI	GGATC	9 cut(s)	374, 676, 731, 744, 883, 1606, 1653, 1666, 1751
BspT107I	GGYRCC	2 cut(s)	1416, 1900
BsrBI	CCGCTC	1 cut(s)	549
BsrDI	GCAATG	1 cut(s)	335
BsrFI	RCCGGY	1 cut(s)	1548
BsrI	ACTGG	4 cut(s)	27, 451, 725, 2042
BssAI	RCCGGY	1 cut(s)	1548
BssECI	CCNNGG	4 cut(s)	179, 834, 1589, 1642
BssNI	GRCGYC	2 cut(s)	63, 1417
BssSI	CACGAG	2 cut(s)	516, 1749
BssT1I	CCWWGG	3 cut(s)	834, 1589, 1642
Bst2BI	CACGAG	2 cut(s)	516, 1749
Bst2UI	CCWGG	2 cut(s)	1377, 1913
Bst4CI	ACNGT	5 cut(s)	1531, 1576, 1586, 1600, 1744
Bst6I	CTCTTC	6 cut(s)	376, 677, 1030, 1125, 1709, 1940
BstACI	GRCGYC	2 cut(s)	63, 1417
BstAPI	GCANNNNNTGC	2 cut(s)	227, 346
BstBAI	YACGTR	1 cut(s)	1066
BstC8I	GCNNGC	4 cut(s)	296, 973, 1046, 1183
BstDEI	CTNAG	3 cut(s)	389, 602, 1497
BstDSI	CCRYGG	1 cut(s)	1642
BstF5I	GGATG	9 cut(s)	22, 221, 628, 652, 916, 930, 1305, 1638, 2009
BstH2I	RGCGCY	1 cut(s)	1420
BstHHI	GCGC	3 cut(s)	959, 1089, 1419
BstMAI	GTCTC	2 cut(s)	1318, 1844
BstNI	CCWGG	2 cut(s)	1377, 1913
BstNSI	RCATGY	4 cut(s)	298, 1185, 1732, 1821
BstSCI	CCNGG	7 cut(s)	66, 178, 468, 1375, 1626, 1886, 1911
BstSFI	CTRYAG	4 cut(s)	299, 453, 611, 1530
BstSLI	GKGCMC	1 cut(s)	523
BstV1I	GCAGC	9 cut(s)	285, 310, 445, 597, 663, 784, 865, 1005, 1129
BstX2I	RGATCY	4 cut(s)	736, 1658, 1756, 1917
BstYI	RGATCY	4 cut(s)	736, 1658, 1756, 1917
BsuI	GTATCC	1 cut(s)	1588
BsuRI	GGCC	5 cut(s)	416, 1077, 1297, 1387, 1846
BtgI	CCRYGG	1 cut(s)	1642
BtgZI	GCGATG	1 cut(s)	1053
BtsCI	GGATG	9 cut(s)	22, 221, 628, 652, 916, 930, 1305, 1638, 2009
BtsI	GCAGTG	3 cut(s)	220, 1135, 1868
BtsIMutI	CAGTG	6 cut(s)	120, 220, 1135, 1527, 1581, 1868
BveI	ACCTGC	1 cut(s)	1888
Cac8I	GCNNGC	4 cut(s)	296, 973, 1046, 1183
CaiI	CAGNNNCTG	1 cut(s)	1145
CciI	TCATGA	1 cut(s)	94
CfoI	GCGC	3 cut(s)	959, 1089, 1419
Cfr10I	RCCGGY	1 cut(s)	1548
Cfr13I	GGNCC	3 cut(s)	415, 1334, 1826
CseI	GACGC	1 cut(s)	949
Csp6I	GTAC	8 cut(s)	241, 444, 788, 1111, 1459, 1546, 1901, 2035
CspCI	CAANNNNNGTGG	2 cut(s)	1749, 1784
CviQI	GTAC	8 cut(s)	241, 444, 788, 1111, 1459, 1546, 1901, 2035
DdeI	CTNAG	3 cut(s)	389, 602, 1497
DinI	GGCGCC	1 cut(s)	1418
DriI	GACNNNNNGTC	1 cut(s)	1584
Eam1104I	CTCTTC	6 cut(s)	376, 677, 1030, 1125, 1709, 1940
Eam1105I	GACNNNNNGTC	1 cut(s)	1584
EarI	CTCTTC	6 cut(s)	376, 677, 1030, 1125, 1709, 1940
EciI	GGCGGA	1 cut(s)	1347
Eco130I	CCWWGG	3 cut(s)	834, 1589, 1642
Eco32I	GATATC	2 cut(s)	1150, 1504
Eco47I	GGWCC	2 cut(s)	1334, 1826
Eco57I	CTGAAG	2 cut(s)	141, 1374
EcoO109I	RGGNCCY	1 cut(s)	415
EcoRII	CCWGG	2 cut(s)	1375, 1911
EcoRV	GATATC	2 cut(s)	1150, 1504
EcoT14I	CCWWGG	3 cut(s)	834, 1589, 1642
EgeI	GGCGCC	1 cut(s)	1418
EheI	GGCGCC	1 cut(s)	1418
ErhI	CCWWGG	3 cut(s)	834, 1589, 1642
FaqI	GGGAC	4 cut(s)	15, 50, 1261, 1433
FauI	CCCGC	1 cut(s)	913
FbaI	TGATCA	1 cut(s)	124
FblI	GTMKAC	2 cut(s)	60, 1974
FokI	GGATG	9 cut(s)	29, 208, 635, 659, 923, 937, 1292, 1625, 2016
FspAI	RTGCGCAY	1 cut(s)	958
FspBI	CTAG	4 cut(s)	572, 713, 1421, 1590
FspI	TGCGCA	1 cut(s)	958
GlaI	GCGC	3 cut(s)	958, 1088, 1418
GsaI	CCCAGC	2 cut(s)	1128, 1375
GsuI	CTGGAG	1 cut(s)	742
HaeII	RGCGCY	1 cut(s)	1420
HaeIII	GGCC	5 cut(s)	416, 1077, 1297, 1387, 1846
HgaI	GACGC	1 cut(s)	949
HhaI	GCGC	3 cut(s)	959, 1089, 1419
Hin1I	GRCGYC	2 cut(s)	63, 1417
Hin6I	GCGC	3 cut(s)	957, 1087, 1417
HinP1I	GCGC	3 cut(s)	957, 1087, 1417
HincII	GTYRAC	3 cut(s)	61, 1537, 1975
HindII	GTYRAC	3 cut(s)	61, 1537, 1975
HindIII	AAGCTT	1 cut(s)	1158
HinfI	GANTC	8 cut(s)	85, 509, 656, 685, 707, 815, 949, 1225
HphI	GGTGA	6 cut(s)	196, 292, 563, 569, 815, 1954
Hpy166II	GTNNAC	5 cut(s)	61, 283, 1527, 1537, 1975
Hpy188III	TCNNGA	7 cut(s)	95, 206, 356, 497, 713, 1921, 1933
Hpy8I	GTNNAC	5 cut(s)	61, 283, 1527, 1537, 1975
Hpy99I	CGWCG	4 cut(s)	65, 758, 1976, 1979
HpyCH4III	ACNGT	5 cut(s)	1531, 1576, 1586, 1600, 1744
HpyCH4IV	ACGT	4 cut(s)	63, 1065, 1446, 1464
HpyF3I	CTNAG	3 cut(s)	389, 602, 1497
HpySE526I	ACGT	4 cut(s)	63, 1065, 1446, 1464
Hsp92I	GRCGYC	2 cut(s)	63, 1417
HspAI	GCGC	3 cut(s)	957, 1087, 1417
KasI	GGCGCC	1 cut(s)	1416
Kpn2I	TCCGGA	1 cut(s)	205
KpnI	GGTACC	1 cut(s)	1904
Ksp22I	TGATCA	1 cut(s)	124
LmnI	GCTCC	3 cut(s)	358, 598, 607
Lsp1109I	GCAGC	9 cut(s)	285, 310, 445, 597, 663, 784, 865, 1005, 1129
LweI	GCATC	3 cut(s)	230, 243, 577
MaeI	CTAG	4 cut(s)	572, 713, 1421, 1590
MaeII	ACGT	4 cut(s)	63, 1065, 1446, 1464
MaeIII	GTNAC	1 cut(s)	1576
MbiI	CCGCTC	1 cut(s)	549
MflI	RGATCY	4 cut(s)	736, 1658, 1756, 1917
MhlI	GDGCHC	2 cut(s)	191, 523
Mly113I	GGCGCC	1 cut(s)	1417
MlyI	GAGTC	5 cut(s)	518, 650, 694, 824, 943
MmeI	TCCRAC	1 cut(s)	843
MroI	TCCGGA	1 cut(s)	205
MroXI	GAANNNNTTC	1 cut(s)	1488
MseI	TTAA	2 cut(s)	1154, 1443
MslI	CAYNNNNRTG	2 cut(s)	194, 405
MspA1I	CMGCKG	3 cut(s)	743, 775, 1142
MspR9I	CCNGG	7 cut(s)	68, 180, 470, 1377, 1628, 1888, 1913
Mva1269I	GAATGC	2 cut(s)	85, 994
MvaI	CCWGG	2 cut(s)	1377, 1913
NarI	GGCGCC	1 cut(s)	1417
NciI	CCSGG	5 cut(s)	68, 180, 470, 1628, 1888
NcoI	CCATGG	1 cut(s)	1642
NlaIV	GGNNCC	6 cut(s)	417, 738, 1418, 1660, 1827, 1902
NmuCI	GTSAC	1 cut(s)	1576
NsbI	TGCGCA	1 cut(s)	958
NspI	RCATGY	4 cut(s)	298, 1185, 1732, 1821
PaeI	GCATGC	2 cut(s)	298, 1185
PagI	TCATGA	1 cut(s)	94
PciI	ACATGT	1 cut(s)	1728
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PctI	GAATGC	2 cut(s)	85, 994
PdmI	GAANNNNTTC	1 cut(s)	1488
PfeI	GAWTC	3 cut(s)	85, 707, 1225
Pfl23II	CGTACG	1 cut(s)	1110
PleI	GAGTC	5 cut(s)	517, 650, 693, 823, 943
PluTI	GGCGCC	1 cut(s)	1420
PpsI	GAGTC	5 cut(s)	517, 650, 693, 823, 943
Ppu21I	YACGTR	1 cut(s)	1066
PscI	ACATGT	1 cut(s)	1728
Psp6I	CCWGG	2 cut(s)	1375, 1911
PspFI	CCCAGC	2 cut(s)	1124, 1371
PspGI	CCWGG	2 cut(s)	1375, 1911
PspLI	CGTACG	1 cut(s)	1110
PspN4I	GGNNCC	6 cut(s)	417, 738, 1418, 1660, 1827, 1902
PspPI	GGNCC	3 cut(s)	415, 1334, 1826
PstI	CTGCAG	2 cut(s)	303, 615
PstNI	CAGNNNCTG	1 cut(s)	1145
PsuI	RGATCY	4 cut(s)	736, 1658, 1756, 1917
PvuII	CAGCTG	2 cut(s)	775, 1142
RsaI	GTAC	8 cut(s)	242, 445, 789, 1112, 1460, 1547, 1902, 2036
RsaNI	GTAC	8 cut(s)	241, 444, 788, 1111, 1459, 1546, 1901, 2035
RseI	CAYNNNNRTG	2 cut(s)	194, 405
SalI	GTCGAC	2 cut(s)	59, 1973
SaqAI	TTAA	2 cut(s)	1154, 1443
Sau96I	GGNCC	3 cut(s)	415, 1334, 1826
ScaI	AGTACT	1 cut(s)	445
SchI	GAGTC	5 cut(s)	518, 650, 694, 824, 943
ScrFI	CCNGG	7 cut(s)	68, 180, 470, 1377, 1628, 1888, 1913
SduI	GDGCHC	2 cut(s)	191, 523
SfaNI	GCATC	3 cut(s)	230, 243, 577
SfcI	CTRYAG	4 cut(s)	299, 453, 611, 1530
SfoI	GGCGCC	1 cut(s)	1418
SgrDI	CGTCGACG	1 cut(s)	1973
SinI	GGWCC	2 cut(s)	1334, 1826
SmiMI	CAYNNNNRTG	2 cut(s)	194, 405
SmlI	CTYRAG	2 cut(s)	236, 1004
SmoI	CTYRAG	2 cut(s)	236, 1004
SphI	GCATGC	2 cut(s)	298, 1185
SsiI	CCGC	6 cut(s)	549, 743, 770, 920, 1332, 1414
SspDI	GGCGCC	1 cut(s)	1416
SspMI	CTAG	4 cut(s)	572, 713, 1421, 1590
StyD4I	CCNGG	7 cut(s)	66, 178, 468, 1375, 1626, 1886, 1911
StyI	CCWWGG	3 cut(s)	834, 1589, 1642
TaaI	ACNGT	5 cut(s)	1531, 1576, 1586, 1600, 1744
TaiI	ACGT	4 cut(s)	66, 1068, 1449, 1467
TaqII	GACCGA	1 cut(s)	1482
TatI	WGTACW	3 cut(s)	240, 443, 1458
TauI	GCSGC	1 cut(s)	1417
TfiI	GAWTC	3 cut(s)	85, 707, 1225
Tru1I	TTAA	2 cut(s)	1154, 1443
Tru9I	TTAA	2 cut(s)	1154, 1443
TscAI	CASTG	6 cut(s)	127, 220, 1135, 1534, 1581, 1868
TseFI	GTSAC	1 cut(s)	1576
TseI	GCWGC	9 cut(s)	298, 323, 458, 610, 651, 772, 853, 1018, 1142
Tsp45I	GTSAC	1 cut(s)	1576
TspDTI	ATGAA	6 cut(s)	33, 83, 212, 573, 1011, 1627
TspGWI	ACGGA	3 cut(s)	34, 206, 1960
TspRI	CASTG	6 cut(s)	127, 220, 1135, 1534, 1581, 1868
VpaK11BI	GGWCC	2 cut(s)	1334, 1826
XapI	RAATTY	3 cut(s)	199, 525, 758
XbaI	TCTAGA	1 cut(s)	712
XceI	RCATGY	4 cut(s)	298, 1185, 1732, 1821
XmaJI	CCTAGG	1 cut(s)	1589
XmiI	GTMKAC	2 cut(s)	60, 1974
XmnI	GAANNNNTTC	1 cut(s)	1488
XspI	CTAG	4 cut(s)	572, 713, 1421, 1590
ZraI	GACGTC	1 cut(s)	64
ZrmI	AGTACT	1 cut(s)	445

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.