Rw5G040420

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000175
Physical Location & Seq
Forward (+)
71319707 .. 71321803
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040420.1

Sequence Viewer

Length: 2097 bp
ATGGATACACTTCTTAAAAGATATTCTGTTCCCATGGAAACATTCGTATTTGATCAGGGCTCAATTTCAAACTACTCTTATCCAGATCATGGTAATGGGTTTGAAGTAAATCATGAACTCATCGACCCACTTTTCCTTCCTACCAACTTGGACCTTCTTAGTGTTTCTAGTACATCTTTGAAGCCGACTTCAGACAGAAATGGTGGTGACAGTAGTGACTACAATAATCCTGTTCTCAAGTATATAAGTGATATTCTTCTCGAAGAGGACATTGAGGACAAGCCTTGTATGTTACAGGACTGTTTGGCCCTCCAAGCTGCTGAGAAATCTTTCTATGATGTCCTTAATCCAAAGGATGCTACAGTACTCAACCAAAGCTTTGAGAACTCAGATGATTACAGGGAGAGTTGTGATAGCAATGGTGGCTCTATTTTTGGTAAGACATATGGGGTTGCTGATCCTTCAGAATCTTGTCATTTCCAGTCTTCTCTTGTTGAGTTTCTATCGGATACCTCCTTGGTTTCTGATTCAAGTTCTAAGCTGCAAAGTCTAGGGAATTTTGGAAGGGTGGAGGAAGCAAACAAGTTCCATGAAAATTTTGAAATCATTGATGCGGAGAGGTACCAATTAATGCTTCAAGGTCCTCAGACACTAATAGGATCAAATTTGGTGTCTGAGCAACAGAGTGATCGGTACAACTCAGCAAATAGATCAGAGGCAAAAAAAAATCGTCAAAGGCAGGATGCTCATTGTCTGGAAGAATGGCGAAGCAACAAGCAGTCAGTTGCATATTTTACTGACGACTCTGAGCCGCAAGAAATGTTTGATAAGGCATTACTCTTTCAAGGTGTGAATCACGAAGCCGAGTGTAGTTCTCATCATGATTCTTTTAATGAGGGAAGTGGGCAGTGCAAAGTATCTAAGCCAGCACGTTCAAAGAGACAAAATAAAAAGGAGAAAGTAGTAGATTTGTGCACAATGTTAACTCAATGCGCGCAAGCTGTTGCAAGTTATGACCAACGAAATGCTAGTGAATTACTGAAGCAGATTAGACAGCACTCTTCTCCCTATGGTGATGCAACCCAGAGATTAGCTCATTACTTTGCTGATGGCCTCGAGGTACGCTTAGCTGGCACTAGAACCCCGTCGTATTCCCCTATTGCAAGCATGCAGATATCAACTGTTGAAATATTGAAAAGTTACCAGACGTATGTCACTTCAACCCCTTTGAAGACCGTGCCACACTTCTTTGCCAATATGACAATTATGAAACTAGCAGAAAAAGCAACAAGTCTTCACATTATTGATTTTGGTACTTCTTATGGTTTCCAATGGCCCTCTCTCATCCAATGTCTTTCGGAGAGACATGGTGGACCTCCTAAGCTTCGTATCACTACAATTGAGCTTCCCCAACCAGGATTTCGACCCACAGAGAGGGTTGAAGAAACTGGTCGTCGCTTAGCAAAGTATTCTGCAAGATTTAGTGTCCCATTTGAGTTCCATGTCATTGCCCAGAAATGGGAAACTGTTGGATTTGATGATCTTAAGATCGACAGAAATGAGCTGATTGTGGTCAACTGTCTACACCGGTTAAAGCACGTTCCTGATGTGACAATGATGGGGAACAGTCCGAGAGATGCTGTCCTAAAGTTGATCAAGAAAATCAATCCAGAAATTTTCATTCATGGGGTTGTCAATGGGGCATACAACTCACCCTTCTTTCTCACACGTTTCAAACAGGCACTCTTCCACTACGATGCACTCTTTAATGTCTTTGAGACCACCATTCCTTGTGAGGATCAACAACGACTGTTTTTCGAAAGAGCAGGATATGGTAGAGACATAATGAATGTAGTGGCGTGTGAGGGACTGGAAATAGTTGAAAGGCCTGAGACATATAAGAAGTGGCAGGTCAGGACTGTGAGGGCTGGGTTCAAGCAGCTCCCATTAGACCAGGAGCTGTTGAAGAAGGTGAAGACTATGTCAAAGTCGATGGGTTATCACAATGATTTTAGTATTGATGAAGACGAGCAGTGGATGCTGCAGGGATGGAAAGGAAGAGTCCTCATGGCTCTTTCCTTCTGGAAACGCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

698

Amino Acids

79.52

Weight (kDa)

5.71

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 326 - 696 6.3e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1115
Acc36I ACCTGC 1 cut(s) 1900
Acc65I GGTACC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 621
AccB7I CCANNNNNTGG 1 cut(s) 89
AccI GTMKAC 1 cut(s) 1582
AccII CGCG 2 cut(s) 995, 2091
AciI CCGC 2 cut(s) 614, 812
AclWI GGATC 3 cut(s) 452, 667, 1806
AcsI RAATTY 4 cut(s) 556, 595, 664, 1674
AcuI CTGAAG 3 cut(s) 174, 447, 1061
AfaI GTAC 6 cut(s) 172, 366, 623, 695, 1122, 1315
AfiI CCNNNNNNNGG 5 cut(s) 89, 1415, 1434, 1518, 1519
AflII CTTAAG 1 cut(s) 1544
AflIII ACRYGT 1 cut(s) 1727
AgeI ACCGGT 1 cut(s) 1587
AjnI CCWGG 2 cut(s) 1414, 1953
AjuI GAANNNNNNNTTGG 4 cut(s) 618, 650, 1404, 1436
Alw21I GWGCWC 1 cut(s) 977
Alw26I GTCTC 5 cut(s) 934, 1357, 1772, 1833, 1886
Alw44I GTGCAC 1 cut(s) 973
AlwI GGATC 3 cut(s) 452, 667, 1806
AlwNI CAGNNNCTG 1 cut(s) 1960
Ama87I CYCGRG 1 cut(s) 1115
AoxI GGCC 4 cut(s) 306, 1111, 1334, 1886
ApaLI GTGCAC 1 cut(s) 973
ApeKI GCWGC 4 cut(s) 317, 541, 1939, 2041
ApoI RAATTY 4 cut(s) 556, 595, 664, 1674
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AseI ATTAAT 1 cut(s) 629
AsiGI ACCGGT 1 cut(s) 1587
Asp700I GAANNNNTTC 1 cut(s) 329
Asp718I GGTACC 1 cut(s) 621
AspLEI GCGC 2 cut(s) 995, 997
AspS9I GGNCC 5 cut(s) 151, 307, 641, 1335, 1373
AsuHPI GGTGA 4 cut(s) 218, 1085, 1704, 1984
AsuII TTCGAA 1 cut(s) 1818
AvaI CYCGRG 1 cut(s) 1115
AvaII GGWCC 3 cut(s) 151, 641, 1373
BaeGI GKGCMC 1 cut(s) 977
BanI GGYRCC 1 cut(s) 621
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 5 cut(s) 477, 1238, 1286, 1982, 2031
Bbv12I GWGCWC 1 cut(s) 977
BbvI GCAGC 4 cut(s) 304, 528, 1951, 2028
BccI CCATC 4 cut(s) 1103, 1612, 1987, 2043
BciT130I CCWGG 2 cut(s) 1416, 1955
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 2 cut(s) 52, 1653
BcoDI GTCTC 5 cut(s) 934, 1357, 1772, 1833, 1886
BfaI CTAG 5 cut(s) 168, 551, 1029, 1137, 1274
BfmI CTRYAG 2 cut(s) 360, 2042
BfrI CTTAAG 1 cut(s) 1544
BfuAI ACCTGC 1 cut(s) 1900
BfuI GTATCC 1 cut(s) 502
BisI GCNGC 5 cut(s) 318, 542, 812, 1940, 2042
BlpI GCTNAGC 2 cut(s) 1126, 1459
BlsI GCNGC 5 cut(s) 319, 543, 813, 1941, 2043
BmcAI AGTACT 1 cut(s) 366
Bme1390I CCNGG 2 cut(s) 1416, 1955
Bme18I GGWCC 3 cut(s) 151, 641, 1373
BmeT110I CYCGRG 1 cut(s) 1115
BmgT120I GGNCC 5 cut(s) 151, 307, 641, 1335, 1373
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 2 cut(s) 1416, 1955
BmsI GCATC 7 cut(s) 346, 601, 733, 1066, 1627, 1747, 2028
BoxI GACNNNNGTC 1 cut(s) 1211
BpiI GAAGAC 5 cut(s) 477, 1238, 1286, 1982, 2031
BplI GAGNNNNNCTC 2 cut(s) 1078, 1110
Bpu10I CCTNAGC 1 cut(s) 1380
Bpu1102I GCTNAGC 2 cut(s) 1126, 1459
Bpu14I TTCGAA 1 cut(s) 1818
BpuEI CTTGAG 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 1772
BsaJI CCNNGG 2 cut(s) 33, 516
BsaWI WCCGGW 1 cut(s) 1587
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 1415, 1434, 1518, 1519
Bse118I RCCGGY 1 cut(s) 1587
Bse1I ACTGG 3 cut(s) 481, 1453, 1875
Bse3DI GCAATG 2 cut(s) 424, 1506
BseBI CCWGG 2 cut(s) 1416, 1955
BseDI CCNNGG 2 cut(s) 33, 516
BseGI GGATG 5 cut(s) 361, 748, 1344, 2043, 2054
BseLI CCNNNNNNNGG 5 cut(s) 89, 1415, 1434, 1518, 1519
BseMI GCAATG 2 cut(s) 424, 1506
BseMII CTCAG 7 cut(s) 312, 402, 659, 666, 714, 798, 1881
BseNI ACTGG 3 cut(s) 481, 1453, 1875
BsePI GCGCGC 1 cut(s) 993
BseSI GKGCMC 1 cut(s) 977
BseXI GCAGC 4 cut(s) 304, 528, 1951, 2028
BseYI CCCAGC 1 cut(s) 1928
Bsh1236I CGCG 2 cut(s) 995, 2091
BshFI GGCC 4 cut(s) 308, 1113, 1336, 1888
BshNI GGYRCC 1 cut(s) 621
BshTI ACCGGT 1 cut(s) 1587
BsiHKAI GWGCWC 1 cut(s) 977
BsiHKCI CYCGRG 1 cut(s) 1115
BsiSI CCGG 1 cut(s) 1588
BslFI GGGAC 2 cut(s) 1472, 1881
BslI CCNNNNNNNGG 5 cut(s) 89, 1415, 1434, 1518, 1519
BsmAI GTCTC 5 cut(s) 934, 1357, 1772, 1833, 1886
BsmFI GGGAC 2 cut(s) 1472, 1881
BsnI GGCC 4 cut(s) 308, 1113, 1336, 1888
Bso31I GGTCTC 1 cut(s) 1772
BsoBI CYCGRG 1 cut(s) 1115
Bsp119I TTCGAA 1 cut(s) 1818
Bsp1286I GDGCHC 2 cut(s) 62, 977
Bsp1720I GCTNAGC 2 cut(s) 1126, 1459
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 2 cut(s) 614, 812
BspANI GGCC 4 cut(s) 308, 1113, 1336, 1888
BspCNI CTCAG 7 cut(s) 313, 401, 658, 667, 713, 799, 1882
BspFNI CGCG 2 cut(s) 995, 2091
BspHI TCATGA 2 cut(s) 112, 880
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 1 cut(s) 2046
BspMI ACCTGC 1 cut(s) 1900
BspPI GGATC 3 cut(s) 452, 667, 1806
BspT104I TTCGAA 1 cut(s) 1818
BspT107I GGYRCC 1 cut(s) 621
BspTI CTTAAG 1 cut(s) 1544
BspTNI GGTCTC 1 cut(s) 1772
BsrDI GCAATG 2 cut(s) 424, 1506
BsrFI RCCGGY 1 cut(s) 1587
BsrI ACTGG 3 cut(s) 481, 1453, 1875
BssAI RCCGGY 1 cut(s) 1587
BssECI CCNNGG 2 cut(s) 33, 516
BssHII GCGCGC 1 cut(s) 993
BssT1I CCWWGG 2 cut(s) 33, 516
Bst2UI CCWGG 2 cut(s) 1416, 1955
Bst6I CTCTTC 4 cut(s) 258, 1066, 1751, 2053
BstAFI CTTAAG 1 cut(s) 1544
BstAPI GCANNNNNTGC 1 cut(s) 2038
BstBI TTCGAA 1 cut(s) 1818
BstC8I GCNNGC 6 cut(s) 927, 995, 999, 1132, 1165, 1169
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 5 cut(s) 361, 748, 1344, 2043, 2054
BstFNI CGCG 2 cut(s) 995, 2091
BstHHI GCGC 2 cut(s) 995, 997
BstMAI GTCTC 5 cut(s) 934, 1357, 1772, 1833, 1886
BstMWI GCNNNNNNNGC 5 cut(s) 314, 423, 1131, 1283, 2038
BstNI CCWGG 2 cut(s) 1416, 1955
BstNSI RCATGY 1 cut(s) 1171
BstPAI GACNNNNGTC 1 cut(s) 1211
BstSCI CCNGG 2 cut(s) 1414, 1953
BstSFI CTRYAG 2 cut(s) 360, 2042
BstSLI GKGCMC 1 cut(s) 977
BstUI CGCG 2 cut(s) 995, 2091
BstV1I GCAGC 4 cut(s) 304, 528, 1951, 2028
BstV2I GAAGAC 5 cut(s) 477, 1238, 1286, 1982, 2031
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 4 cut(s) 308, 1113, 1336, 1888
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 5 cut(s) 361, 748, 1344, 2043, 2054
BtsI GCAGTG 2 cut(s) 914, 2039
BtsIMutI CAGTG 2 cut(s) 914, 2039
BveI ACCTGC 1 cut(s) 1900
Cac8I GCNNGC 6 cut(s) 927, 995, 999, 1132, 1165, 1169
CaiI CAGNNNCTG 1 cut(s) 1960
CciI TCATGA 2 cut(s) 112, 880
CfoI GCGC 2 cut(s) 995, 997
Cfr10I RCCGGY 1 cut(s) 1587
Cfr13I GGNCC 5 cut(s) 151, 307, 641, 1335, 1373
Csp6I GTAC 6 cut(s) 171, 365, 622, 694, 1121, 1314
CspAI ACCGGT 1 cut(s) 1587
CviQI GTAC 6 cut(s) 171, 365, 622, 694, 1121, 1314
Eam1104I CTCTTC 4 cut(s) 258, 1066, 1751, 2053
EarI CTCTTC 4 cut(s) 258, 1066, 1751, 2053
Eco130I CCWWGG 2 cut(s) 33, 516
Eco147I AGGCCT 1 cut(s) 1888
Eco24I GRGCYC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 1772
Eco32I GATATC 1 cut(s) 1176
Eco47I GGWCC 3 cut(s) 151, 641, 1373
Eco57I CTGAAG 3 cut(s) 174, 447, 1061
Eco88I CYCGRG 1 cut(s) 1115
EcoO109I RGGNCCY 1 cut(s) 641
EcoRII CCWGG 2 cut(s) 1414, 1953
EcoRV GATATC 1 cut(s) 1176
EcoT14I CCWWGG 2 cut(s) 33, 516
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 2 cut(s) 33, 516
FaqI GGGAC 2 cut(s) 1472, 1881
FauNDI CATATG 1 cut(s) 445
FbaI TGATCA 2 cut(s) 52, 1653
FblI GTMKAC 1 cut(s) 1582
Fnu4HI GCNGC 5 cut(s) 318, 542, 812, 1940, 2042
FokI GGATG 5 cut(s) 368, 755, 1331, 2050, 2061
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 5 cut(s) 318, 542, 812, 1940, 2042
FspBI CTAG 5 cut(s) 168, 551, 1029, 1137, 1274
GlaI GCGC 2 cut(s) 994, 996
GluI GCNGC 5 cut(s) 318, 542, 812, 1940, 2042
GsaI CCCAGC 1 cut(s) 1932
HaeIII GGCC 4 cut(s) 308, 1113, 1336, 1888
HapII CCGG 1 cut(s) 1588
HhaI GCGC 2 cut(s) 995, 997
Hin6I GCGC 2 cut(s) 993, 995
HinP1I GCGC 2 cut(s) 993, 995
HincII GTYRAC 2 cut(s) 984, 1576
HindII GTYRAC 2 cut(s) 984, 1576
HindIII AAGCTT 2 cut(s) 376, 1382
HinfI GANTC 6 cut(s) 467, 527, 803, 853, 884, 2061
HpaI GTTAAC 1 cut(s) 984
HpaII CCGG 1 cut(s) 1588
HphI GGTGA 4 cut(s) 218, 1085, 1704, 1984
Hpy166II GTNNAC 5 cut(s) 975, 984, 1373, 1576, 1583
Hpy8I GTNNAC 5 cut(s) 975, 984, 1373, 1576, 1583
Hpy99I CGWCG 2 cut(s) 1150, 1458
HpyAV CCTTC 7 cut(s) 146, 164, 471, 558, 1726, 1963, 2089
HpyCH4IV ACGT 4 cut(s) 931, 1208, 1599, 1729
HpyF10VI GCNNNNNNNGC 5 cut(s) 314, 423, 1131, 1283, 2038
HpySE526I ACGT 4 cut(s) 931, 1208, 1599, 1729
HspAI GCGC 2 cut(s) 993, 995
KpnI GGTACC 1 cut(s) 625
Ksp22I TGATCA 2 cut(s) 52, 1653
KspAI GTTAAC 1 cut(s) 984
LmnI GCTCC 2 cut(s) 1947, 1957
Lsp1109I GCAGC 4 cut(s) 304, 528, 1951, 2028
LweI GCATC 7 cut(s) 346, 601, 733, 1066, 1627, 1747, 2028
MaeI CTAG 5 cut(s) 168, 551, 1029, 1137, 1274
MaeII ACGT 4 cut(s) 931, 1208, 1599, 1729
MaeIII GTNAC 6 cut(s) 206, 215, 291, 1199, 1213, 1609
MfeI CAATTG 1 cut(s) 1398
MhlI GDGCHC 2 cut(s) 62, 977
MluCI AATT 9 cut(s) 63, 556, 595, 626, 664, 1034, 1263, 1398, 1674
MlyI GAGTC 2 cut(s) 797, 2070
MmeI TCCRAC 1 cut(s) 1510
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 8 cut(s) 15, 345, 629, 891, 983, 1545, 1592, 1767
MslI CAYNNNNRTG 2 cut(s) 93, 1755
MspCI CTTAAG 1 cut(s) 1544
MspI CCGG 1 cut(s) 1588
MspR9I CCNGG 2 cut(s) 1416, 1955
MunI CAATTG 1 cut(s) 1398
MvaI CCWGG 2 cut(s) 1416, 1955
MvnI CGCG 2 cut(s) 995, 2091
MwoI GCNNNNNNNGC 5 cut(s) 314, 423, 1131, 1283, 2038
NcoI CCATGG 1 cut(s) 33
NdeI CATATG 1 cut(s) 445
NlaIV GGNNCC 1 cut(s) 623
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 4 cut(s) 206, 215, 1213, 1609
NspI RCATGY 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 1818
PaeI GCATGC 1 cut(s) 1171
PaeR7I CTCGAG 1 cut(s) 1115
PagI TCATGA 2 cut(s) 112, 880
PauI GCGCGC 1 cut(s) 993
PceI AGGCCT 1 cut(s) 1888
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 4 cut(s) 467, 527, 853, 884
PflFI GACNNNGTC 1 cut(s) 1981
PflMI CCANNNNNTGG 1 cut(s) 89
PinAI ACCGGT 1 cut(s) 1587
PkrI GCNGC 5 cut(s) 319, 543, 813, 1941, 2043
PleI GAGTC 2 cut(s) 797, 2069
PpsI GAGTC 2 cut(s) 797, 2069
PpuMI RGGWCCY 1 cut(s) 641
PshAI GACNNNNGTC 1 cut(s) 1211
PshBI ATTAAT 1 cut(s) 629
Psp5II RGGWCCY 1 cut(s) 641
Psp6I CCWGG 2 cut(s) 1414, 1953
PspFI CCCAGC 1 cut(s) 1928
PspGI CCWGG 2 cut(s) 1414, 1953
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 5 cut(s) 151, 307, 641, 1335, 1373
PspPPI RGGWCCY 1 cut(s) 641
PspXI VCTCGAGB 1 cut(s) 1115
PstI CTGCAG 1 cut(s) 2046
PstNI CAGNNNCTG 1 cut(s) 1960
PsyI GACNNNGTC 1 cut(s) 1981
PteI GCGCGC 1 cut(s) 993
RsaI GTAC 6 cut(s) 172, 366, 623, 695, 1122, 1315
RsaNI GTAC 6 cut(s) 171, 365, 622, 694, 1121, 1314
RseI CAYNNNNRTG 2 cut(s) 93, 1755
SaqAI TTAA 8 cut(s) 15, 345, 629, 891, 983, 1545, 1592, 1767
SatI GCNGC 5 cut(s) 318, 542, 812, 1940, 2042
Sau96I GGNCC 5 cut(s) 151, 307, 641, 1335, 1373
ScaI AGTACT 1 cut(s) 366
SchI GAGTC 2 cut(s) 797, 2070
ScrFI CCNGG 2 cut(s) 1416, 1955
SduI GDGCHC 2 cut(s) 62, 977
SfaNI GCATC 7 cut(s) 346, 601, 733, 1066, 1627, 1747, 2028
SfcI CTRYAG 2 cut(s) 360, 2042
Sfr274I CTCGAG 1 cut(s) 1115
SfuI TTCGAA 1 cut(s) 1818
SinI GGWCC 3 cut(s) 151, 641, 1373
SlaI CTCGAG 1 cut(s) 1115
SmiMI CAYNNNNRTG 2 cut(s) 93, 1755
SmlI CTYRAG 3 cut(s) 236, 1115, 1544
SmoI CTYRAG 3 cut(s) 236, 1115, 1544
SphI GCATGC 1 cut(s) 1171
Sse9I AATT 9 cut(s) 63, 556, 595, 626, 664, 1034, 1263, 1398, 1674
SseBI AGGCCT 1 cut(s) 1888
SsiI CCGC 2 cut(s) 614, 812
SspI AATATT 1 cut(s) 1191
SspMI CTAG 5 cut(s) 168, 551, 1029, 1137, 1274
StuI AGGCCT 1 cut(s) 1888
StyD4I CCNGG 2 cut(s) 1414, 1953
StyI CCWWGG 2 cut(s) 33, 516
TaiI ACGT 4 cut(s) 934, 1211, 1602, 1732
TaqI TCGA 7 cut(s) 123, 261, 1116, 1423, 1551, 1818, 1991
TasI AATT 9 cut(s) 63, 556, 595, 626, 664, 1034, 1263, 1398, 1674
TatI WGTACW 2 cut(s) 170, 364
TauI GCSGC 1 cut(s) 814
TfiI GAWTC 4 cut(s) 467, 527, 853, 884
Tru1I TTAA 8 cut(s) 15, 345, 629, 891, 983, 1545, 1592, 1767
Tru9I TTAA 8 cut(s) 15, 345, 629, 891, 983, 1545, 1592, 1767
TscAI CASTG 2 cut(s) 914, 2039
TseFI GTSAC 4 cut(s) 206, 215, 1213, 1609
TseI GCWGC 4 cut(s) 317, 541, 1939, 2041
Tsp45I GTSAC 4 cut(s) 206, 215, 1213, 1609
TspDTI ATGAA 7 cut(s) 129, 606, 1283, 1669, 1673, 1862, 2037
TspRI CASTG 2 cut(s) 914, 2039
Tth111I GACNNNGTC 1 cut(s) 1981
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 1544
VneI GTGCAC 1 cut(s) 973
VpaK11BI GGWCC 3 cut(s) 151, 641, 1373
VspI ATTAAT 1 cut(s) 629
XapI RAATTY 4 cut(s) 556, 595, 664, 1674
XceI RCATGY 1 cut(s) 1171
XhoI CTCGAG 1 cut(s) 1115
XmiI GTMKAC 1 cut(s) 1582
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 5 cut(s) 168, 551, 1029, 1137, 1274
ZrmI AGTACT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.