Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1042 |
| AccB1I | GGYRCC | 1 cut(s) | 883 |
| AccII | CGCG | 3 cut(s) | 195, 325, 871 |
| AciI | CCGC | 3 cut(s) | 97, 611, 871 |
| AclWI | GGATC | 1 cut(s) | 690 |
| AcoI | YGGCCR | 1 cut(s) | 536 |
| AcsI | RAATTY | 1 cut(s) | 117 |
| AcyI | GRCGYC | 1 cut(s) | 884 |
| AdeI | CACNNNGTG | 1 cut(s) | 409 |
| AfaI | GTAC | 2 cut(s) | 370, 446 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 617, 679 |
| AgeI | ACCGGT | 1 cut(s) | 1065 |
| AgsI | TTSAA | 3 cut(s) | 433, 1025, 1078 |
| AjnI | CCWGG | 3 cut(s) | 405, 568, 616 |
| AluBI | AGCT | 4 cut(s) | 203, 296, 401, 607 |
| AluI | AGCT | 4 cut(s) | 203, 296, 401, 607 |
| Alw21I | GWGCWC | 2 cut(s) | 179, 609 |
| Alw26I | GTCTC | 3 cut(s) | 559, 771, 1028 |
| Alw44I | GTGCAC | 1 cut(s) | 175 |
| AlwI | GGATC | 1 cut(s) | 690 |
| AoxI | GGCC | 2 cut(s) | 536, 1028 |
| ApaLI | GTGCAC | 1 cut(s) | 175 |
| ApeKI | GCWGC | 1 cut(s) | 256 |
| ApoI | RAATTY | 1 cut(s) | 117 |
| AsiGI | ACCGGT | 1 cut(s) | 1065 |
| AspLEI | GCGC | 5 cut(s) | 197, 325, 327, 729, 886 |
| AspS9I | GGNCC | 1 cut(s) | 575 |
| AsuHPI | GGTGA | 4 cut(s) | 287, 395, 775, 1055 |
| AsuII | TTCGAA | 1 cut(s) | 960 |
| AvaII | GGWCC | 1 cut(s) | 575 |
| BaeGI | GKGCMC | 1 cut(s) | 179 |
| BanI | GGYRCC | 1 cut(s) | 883 |
| BanII | GRGCYC | 1 cut(s) | 609 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 428, 460 |
| BauI | CACGAG | 1 cut(s) | 38 |
| Bbv12I | GWGCWC | 2 cut(s) | 179, 609 |
| BbvI | GCAGC | 1 cut(s) | 268 |
| BccI | CCATC | 1 cut(s) | 74 |
| BceAI | ACGGC | 2 cut(s) | 523, 528 |
| BciT130I | CCWGG | 3 cut(s) | 407, 570, 618 |
| BciVI | GTATCC | 1 cut(s) | 557 |
| BclI | TGATCA | 3 cut(s) | 61, 240, 795 |
| BcoDI | GTCTC | 3 cut(s) | 559, 771, 1028 |
| BfaI | CTAG | 3 cut(s) | 317, 476, 1013 |
| BfoI | RGCGCY | 1 cut(s) | 887 |
| BfuAI | ACCTGC | 1 cut(s) | 1042 |
| BfuI | GTATCC | 1 cut(s) | 557 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 320 |
| BisI | GCNGC | 1 cut(s) | 257 |
| BlsI | GCNGC | 1 cut(s) | 258 |
| Bme1390I | CCNGG | 3 cut(s) | 407, 570, 618 |
| Bme18I | GGWCC | 1 cut(s) | 575 |
| BmgT120I | GGNCC | 1 cut(s) | 575 |
| BmiI | GGNNCC | 2 cut(s) | 337, 885 |
| BmrFI | CCNGG | 3 cut(s) | 407, 570, 618 |
| BmrI | ACTGGG | 1 cut(s) | 264 |
| BmsI | GCATC | 1 cut(s) | 19 |
| BmuI | ACTGGG | 1 cut(s) | 264 |
| BpmI | CTGGAG | 1 cut(s) | 322 |
| Bpu14I | TTCGAA | 1 cut(s) | 960 |
| BsaBI | GATNNNNATC | 2 cut(s) | 33, 945 |
| BsaHI | GRCGYC | 1 cut(s) | 884 |
| BsaI | GGTCTC | 1 cut(s) | 559 |
| BsaWI | WCCGGW | 1 cut(s) | 1065 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 617, 679 |
| Bse118I | RCCGGY | 1 cut(s) | 1065 |
| Bse1I | ACTGG | 1 cut(s) | 270 |
| Bse3DI | GCAATG | 1 cut(s) | 648 |
| Bse8I | GATNNNNATC | 2 cut(s) | 33, 945 |
| BseBI | CCWGG | 3 cut(s) | 407, 570, 618 |
| BseGI | GGATG | 1 cut(s) | 34 |
| BseJI | GATNNNNATC | 2 cut(s) | 33, 945 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 617, 679 |
| BseMI | GCAATG | 1 cut(s) | 648 |
| BseMII | CTCAG | 1 cut(s) | 668 |
| BseNI | ACTGG | 1 cut(s) | 270 |
| BsePI | GCGCGC | 1 cut(s) | 323 |
| BseRI | GAGGAG | 1 cut(s) | 348 |
| BseSI | GKGCMC | 1 cut(s) | 179 |
| BseXI | GCAGC | 1 cut(s) | 268 |
| Bsh1236I | CGCG | 3 cut(s) | 195, 325, 871 |
| BshFI | GGCC | 2 cut(s) | 538, 1030 |
| BshNI | GGYRCC | 1 cut(s) | 883 |
| BshTI | ACCGGT | 1 cut(s) | 1065 |
| BsiHKAI | GWGCWC | 2 cut(s) | 179, 609 |
| BsiSI | CCGG | 2 cut(s) | 1031, 1066 |
| BslFI | GGGAC | 2 cut(s) | 856, 1023 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 617, 679 |
| BsmAI | GTCTC | 3 cut(s) | 559, 771, 1028 |
| BsmFI | GGGAC | 2 cut(s) | 856, 1023 |
| BsnI | GGCC | 2 cut(s) | 538, 1030 |
| Bso31I | GGTCTC | 1 cut(s) | 559 |
| Bsp119I | TTCGAA | 1 cut(s) | 960 |
| Bsp1286I | GDGCHC | 2 cut(s) | 179, 609 |
| Bsp143I | GATC | 4 cut(s) | 61, 240, 682, 795 |
| BspACI | CCGC | 3 cut(s) | 97, 611, 871 |
| BspANI | GGCC | 2 cut(s) | 538, 1030 |
| BspCNI | CTCAG | 1 cut(s) | 667 |
| BspFNI | CGCG | 3 cut(s) | 195, 325, 871 |
| BspHI | TCATGA | 1 cut(s) | 58 |
| BspLI | GGNNCC | 2 cut(s) | 337, 885 |
| BspMI | ACCTGC | 1 cut(s) | 1042 |
| BspPI | GGATC | 1 cut(s) | 690 |
| BspT104I | TTCGAA | 1 cut(s) | 960 |
| BspT107I | GGYRCC | 1 cut(s) | 883 |
| BspTNI | GGTCTC | 1 cut(s) | 559 |
| BsrDI | GCAATG | 1 cut(s) | 648 |
| BsrFI | RCCGGY | 1 cut(s) | 1065 |
| BsrI | ACTGG | 1 cut(s) | 270 |
| BssAI | RCCGGY | 1 cut(s) | 1065 |
| BssHII | GCGCGC | 1 cut(s) | 323 |
| BssMI | GATC | 4 cut(s) | 61, 240, 682, 795 |
| BssNI | GRCGYC | 1 cut(s) | 884 |
| BssSI | CACGAG | 1 cut(s) | 38 |
| Bst2BI | CACGAG | 1 cut(s) | 38 |
| Bst2UI | CCWGG | 3 cut(s) | 407, 570, 618 |
| Bst4CI | ACNGT | 3 cut(s) | 722, 757, 784 |
| BstACI | GRCGYC | 1 cut(s) | 884 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 485 |
| BstBI | TTCGAA | 1 cut(s) | 960 |
| BstC8I | GCNNGC | 5 cut(s) | 133, 211, 325, 334, 1003 |
| BstDEI | CTNAG | 2 cut(s) | 561, 654 |
| BstF5I | GGATG | 1 cut(s) | 34 |
| BstFNI | CGCG | 3 cut(s) | 195, 325, 871 |
| BstH2I | RGCGCY | 1 cut(s) | 887 |
| BstHHI | GCGC | 5 cut(s) | 197, 325, 327, 729, 886 |
| BstKTI | GATC | 4 cut(s) | 64, 243, 685, 798 |
| BstMAI | GTCTC | 3 cut(s) | 559, 771, 1028 |
| BstMBI | GATC | 4 cut(s) | 61, 240, 682, 795 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 320, 329, 333, 485 |
| BstNI | CCWGG | 3 cut(s) | 407, 570, 618 |
| BstSCI | CCNGG | 3 cut(s) | 405, 568, 616 |
| BstSLI | GKGCMC | 1 cut(s) | 179 |
| BstUI | CGCG | 3 cut(s) | 195, 325, 871 |
| BstV1I | GCAGC | 1 cut(s) | 268 |
| BstX2I | RGATCY | 1 cut(s) | 682 |
| BstYI | RGATCY | 1 cut(s) | 682 |
| BsuI | GTATCC | 1 cut(s) | 557 |
| BsuRI | GGCC | 2 cut(s) | 538, 1030 |
| BtsCI | GGATG | 1 cut(s) | 34 |
| BtsIMutI | CAGTG | 2 cut(s) | 277, 780 |
| BveI | ACCTGC | 1 cut(s) | 1042 |
| Cac8I | GCNNGC | 5 cut(s) | 133, 211, 325, 334, 1003 |
| CciI | TCATGA | 1 cut(s) | 58 |
| CfoI | GCGC | 5 cut(s) | 197, 325, 327, 729, 886 |
| Cfr10I | RCCGGY | 1 cut(s) | 1065 |
| Cfr13I | GGNCC | 1 cut(s) | 575 |
| CsiI | ACCWGGT | 3 cut(s) | 405, 568, 616 |
| Csp6I | GTAC | 2 cut(s) | 369, 445 |
| CspAI | ACCGGT | 1 cut(s) | 1065 |
| CviAII | CATG | 3 cut(s) | 59, 215, 423 |
| CviQI | GTAC | 2 cut(s) | 369, 445 |
| DdeI | CTNAG | 2 cut(s) | 561, 654 |
| DinI | GGCGCC | 1 cut(s) | 885 |
| DpnI | GATC | 4 cut(s) | 63, 242, 684, 797 |
| DpnII | GATC | 4 cut(s) | 61, 240, 682, 795 |
| DraIII | CACNNNGTG | 1 cut(s) | 409 |
| EaeI | YGGCCR | 1 cut(s) | 536 |
| Ecl136II | GAGCTC | 1 cut(s) | 607 |
| Eco24I | GRGCYC | 1 cut(s) | 609 |
| Eco31I | GGTCTC | 1 cut(s) | 559 |
| Eco47I | GGWCC | 1 cut(s) | 575 |
| Eco53kI | GAGCTC | 1 cut(s) | 607 |
| EcoICRI | GAGCTC | 1 cut(s) | 607 |
| EcoRII | CCWGG | 3 cut(s) | 405, 568, 616 |
| EcoT38I | GRGCYC | 1 cut(s) | 609 |
| EgeI | GGCGCC | 1 cut(s) | 885 |
| EheI | GGCGCC | 1 cut(s) | 885 |
| FaeI | CATG | 3 cut(s) | 62, 218, 426 |
| FaqI | GGGAC | 2 cut(s) | 856, 1023 |
| FatI | CATG | 3 cut(s) | 58, 214, 422 |
| FauI | CCCGC | 1 cut(s) | 618 |
| FbaI | TGATCA | 3 cut(s) | 61, 240, 795 |
| Fnu4HI | GCNGC | 1 cut(s) | 257 |
| FokI | GGATG | 1 cut(s) | 41 |
| FriOI | GRGCYC | 1 cut(s) | 609 |
| Fsp4HI | GCNGC | 1 cut(s) | 257 |
| FspBI | CTAG | 3 cut(s) | 317, 476, 1013 |
| GlaI | GCGC | 5 cut(s) | 196, 324, 326, 728, 885 |
| GluI | GCNGC | 1 cut(s) | 257 |
| GsuI | CTGGAG | 1 cut(s) | 322 |
| HaeII | RGCGCY | 1 cut(s) | 887 |
| HaeIII | GGCC | 2 cut(s) | 538, 1030 |
| HapII | CCGG | 2 cut(s) | 1031, 1066 |
| HhaI | GCGC | 5 cut(s) | 197, 325, 327, 729, 886 |
| Hin1I | GRCGYC | 1 cut(s) | 884 |
| Hin1II | CATG | 3 cut(s) | 62, 218, 426 |
| Hin6I | GCGC | 5 cut(s) | 195, 323, 325, 727, 884 |
| HinP1I | GCGC | 5 cut(s) | 195, 323, 325, 727, 884 |
| HincII | GTYRAC | 2 cut(s) | 694, 718 |
| HindII | GTYRAC | 2 cut(s) | 694, 718 |
| HinfI | GANTC | 2 cut(s) | 34, 187 |
| HpaI | GTTAAC | 1 cut(s) | 718 |
| HpaII | CCGG | 2 cut(s) | 1031, 1066 |
| HphI | GGTGA | 4 cut(s) | 287, 395, 775, 1055 |
| Hpy166II | GTNNAC | 6 cut(s) | 177, 236, 499, 575, 694, 718 |
| Hpy188I | TCNGA | 3 cut(s) | 657, 674, 878 |
| Hpy188III | TCNNGA | 8 cut(s) | 26, 38, 59, 244, 339, 433, 799, 812 |
| Hpy8I | GTNNAC | 6 cut(s) | 177, 236, 499, 575, 694, 718 |
| HpyAV | CCTTC | 2 cut(s) | 868, 898 |
| HpyCH4III | ACNGT | 3 cut(s) | 722, 757, 784 |
| HpyCH4V | TGCA | 1 cut(s) | 177 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 320, 329, 333, 485 |
| HpyF3I | CTNAG | 2 cut(s) | 561, 654 |
| Hsp92I | GRCGYC | 1 cut(s) | 884 |
| Hsp92II | CATG | 3 cut(s) | 62, 218, 426 |
| HspAI | GCGC | 5 cut(s) | 195, 323, 325, 727, 884 |
| KasI | GGCGCC | 1 cut(s) | 883 |
| Ksp22I | TGATCA | 3 cut(s) | 61, 240, 795 |
| KspAI | GTTAAC | 1 cut(s) | 718 |
| Kzo9I | GATC | 4 cut(s) | 61, 240, 682, 795 |
| LmnI | GCTCC | 3 cut(s) | 99, 341, 612 |
| Lsp1109I | GCAGC | 1 cut(s) | 268 |
| LweI | GCATC | 1 cut(s) | 19 |
| MabI | ACCWGGT | 3 cut(s) | 405, 568, 616 |
| MaeI | CTAG | 3 cut(s) | 317, 476, 1013 |
| MaeIII | GTNAC | 2 cut(s) | 415, 865 |
| MalI | GATC | 4 cut(s) | 63, 242, 684, 797 |
| MboI | GATC | 4 cut(s) | 61, 240, 682, 795 |
| MboII | GAAGA | 5 cut(s) | 259, 442, 448, 578, 950 |
| MfeI | CAATTG | 1 cut(s) | 600 |
| MflI | RGATCY | 1 cut(s) | 682 |
| MhlI | GDGCHC | 2 cut(s) | 179, 609 |
| MluCI | AATT | 4 cut(s) | 117, 465, 600, 850 |
| Mly113I | GGCGCC | 1 cut(s) | 884 |
| MlyI | GAGTC | 1 cut(s) | 181 |
| MmeI | TCCRAC | 1 cut(s) | 574 |
| MseI | TTAA | 5 cut(s) | 365, 395, 687, 717, 734 |
| MslI | CAYNNNNRTG | 1 cut(s) | 182 |
| MspI | CCGG | 2 cut(s) | 1031, 1066 |
| MspR9I | CCNGG | 3 cut(s) | 407, 570, 618 |
| MunI | CAATTG | 1 cut(s) | 600 |
| MvaI | CCWGG | 3 cut(s) | 407, 570, 618 |
| MvnI | CGCG | 3 cut(s) | 195, 325, 871 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 320, 329, 333, 485 |
| NarI | GGCGCC | 1 cut(s) | 884 |
| NdeII | GATC | 4 cut(s) | 61, 240, 682, 795 |
| NlaIII | CATG | 3 cut(s) | 62, 218, 426 |
| NlaIV | GGNNCC | 2 cut(s) | 337, 885 |
| NmuCI | GTSAC | 1 cut(s) | 865 |
| NspV | TTCGAA | 1 cut(s) | 960 |
| PagI | TCATGA | 1 cut(s) | 58 |
| PauI | GCGCGC | 1 cut(s) | 323 |
| PfeI | GAWTC | 1 cut(s) | 34 |
| PflFI | GACNNNGTC | 1 cut(s) | 782 |
| PinAI | ACCGGT | 1 cut(s) | 1065 |
| PkrI | GCNGC | 1 cut(s) | 258 |
| PleI | GAGTC | 1 cut(s) | 181 |
| PluTI | GGCGCC | 1 cut(s) | 887 |
| PpsI | GAGTC | 1 cut(s) | 181 |
| Psp124BI | GAGCTC | 1 cut(s) | 609 |
| Psp6I | CCWGG | 3 cut(s) | 405, 568, 616 |
| PspGI | CCWGG | 3 cut(s) | 405, 568, 616 |
| PspN4I | GGNNCC | 2 cut(s) | 337, 885 |
| PspPI | GGNCC | 1 cut(s) | 575 |
| PsuI | RGATCY | 1 cut(s) | 682 |
| PsyI | GACNNNGTC | 1 cut(s) | 782 |
| PteI | GCGCGC | 1 cut(s) | 323 |
| RsaI | GTAC | 2 cut(s) | 370, 446 |
| RsaNI | GTAC | 2 cut(s) | 369, 445 |
| RseI | CAYNNNNRTG | 1 cut(s) | 182 |
| SacI | GAGCTC | 1 cut(s) | 609 |
| SaqAI | TTAA | 5 cut(s) | 365, 395, 687, 717, 734 |
| SatI | GCNGC | 1 cut(s) | 257 |
| Sau3AI | GATC | 4 cut(s) | 61, 240, 682, 795 |
| Sau96I | GGNCC | 1 cut(s) | 575 |
| SchI | GAGTC | 1 cut(s) | 181 |
| ScrFI | CCNGG | 3 cut(s) | 407, 570, 618 |
| SduI | GDGCHC | 2 cut(s) | 179, 609 |
| SexAI | ACCWGGT | 3 cut(s) | 405, 568, 616 |
| SfaNI | GCATC | 1 cut(s) | 19 |
| SfoI | GGCGCC | 1 cut(s) | 885 |
| SfuI | TTCGAA | 1 cut(s) | 960 |
| SinI | GGWCC | 1 cut(s) | 575 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 182 |
| Sse9I | AATT | 4 cut(s) | 117, 465, 600, 850 |
| SsiI | CCGC | 3 cut(s) | 97, 611, 871 |
| SspDI | GGCGCC | 1 cut(s) | 883 |
| SspMI | CTAG | 3 cut(s) | 317, 476, 1013 |
| SstI | GAGCTC | 1 cut(s) | 609 |
| StyD4I | CCNGG | 3 cut(s) | 405, 568, 616 |
| TaaI | ACNGT | 3 cut(s) | 722, 757, 784 |
| TaqI | TCGA | 2 cut(s) | 625, 960 |
| TasI | AATT | 4 cut(s) | 117, 465, 600, 850 |
| TatI | WGTACW | 1 cut(s) | 444 |
| TfiI | GAWTC | 1 cut(s) | 34 |
| Tru1I | TTAA | 5 cut(s) | 365, 395, 687, 717, 734 |
| Tru9I | TTAA | 5 cut(s) | 365, 395, 687, 717, 734 |
| TscAI | CASTG | 2 cut(s) | 277, 787 |
| TseFI | GTSAC | 1 cut(s) | 865 |
| TseI | GCWGC | 1 cut(s) | 256 |
| Tsp45I | GTSAC | 1 cut(s) | 865 |
| TspDTI | ATGAA | 7 cut(s) | 47, 411, 485, 765, 811, 957, 1004 |
| TspGWI | ACGGA | 1 cut(s) | 843 |
| TspRI | CASTG | 2 cut(s) | 277, 787 |
| Tth111I | GACNNNGTC | 1 cut(s) | 782 |
| VneI | GTGCAC | 1 cut(s) | 175 |
| VpaK11BI | GGWCC | 1 cut(s) | 575 |
| XapI | RAATTY | 1 cut(s) | 117 |
| XspI | CTAG | 3 cut(s) | 317, 476, 1013 |