Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0000175

Physical Location & Seq

Genomic Coordinates Reverse (-)

6372771 .. 6374399

Gene Structure 1629 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g08270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGCTGCTAAGGCAGACTCAGTTTTCGATTCTTCAGAAACCCTTTTGGTTTCAAACTCAACTTCTGGGAATTTTGGAGGAGTTGGGGAGGCAGGAAATCTCGTTCCAAACCTGAAACATGGAATCATTGACTTGGAGAGGTACCAATTAGTTCCCCCGGGCGATAACACACTGAGGAGGAATTCCGCACCAGAGCCAGAGAATGATGGCAACATCTCAGCAAACAGATTGAAGGGAAAGAAGAATCATCGAAGGGAGGATGGTGATTATGCAGATGGAGGGAGAAGCAACAAGCAGTCAGCTGCTTTAGGCGACGACACTGAGCCACAAGAAATGTTTGATAGGGTATTACTCTGTCATGTGAATCATACGCATGCGTCTTGTATTCGTGACAAACCTTTGAGCAGTGAGGGAAGTGAGAAGTTGCAGCCTAAAAAACAGTCAAAAGGGTCTAAAACAACACGCTTGAAGAAACAGAATAACTACCAGGAAGTTGTGGATATTAGCACCCTGCTAACTCAATGTGCACAGGCCACGGCAAGCTATGACCAACGAAGTGCGAGTGAACTACTGAAGCAGATAAGGAAGCACTCTTCCCCCTCTGGTGATGCAACCGAGAGATTAGCTCATTACTTTGCTGATGGCCTAGAGGCGCGCTTGGTGGGAGCCAGAATCCCATCATATTCACCTCTTCTGAGTATACAGACACCAGCCGCTGAAATCCTAAAAGCCCACCAGATGTATGTTAATTATTGCCCTTTCAAGCATATGTTACACTTCTTTTCCAACAGGACAATTATTAAACTAGCTGAAAAAGCAACAAGGCTTCACATTGTTGATTTCGGTATTTCGTATGGTTTCCAATGGCCTTGCCTTATCCAACGCCTCTCGGAGAGAAATGGTGGACCTCCTAGTATACGTTTGACAGCAATTGAGCTTCCCCAACCAGGTTTTCGACCGACAGAAAGAGTTGAGGAGACGGGGCGTCGCTTGAAAAAATATGCTGCAAGATTTAATGTCCAGTTTGAGTACAAGGTCTTTGCACAGAAATGGGAGACTATCCAACTTGAGGATCTTAAAATTGACAGAAATGAGCTAACTGTGGTTAACTGCATGCATCGGTTAAAGCACATACCTGATGAAACCGTGGTGGTGAGCAGTCCGAGGGACCTTGTCCTAAAGTTGATCAGAAGAATTAATCCGGACCTTTTCATTCACGGAGTTGTCAACGGGACATACAATTCACCCTTCTTTGTCACACGGTTCAAAGAGGCACTCTACCACTTCTCTGCTATGTTTGATATGTTTGAGGCCACTATTCCTCGCGAAGATGAGCAGCGGATGATGTTTGAAAAGGCAATATATGGCAAAGACATAATGAATGTCGTCGCGTGTGAAGGACTGGAACGAGTTGAAAGGCCCGAGACATACAAGCAGTGGCAGGTTAGATATCATCGGGCTGGGTTCAAGCAGGTGCCGTTAAACCAGGAGCTCTTGAAAAGAGTGAAGATTATGCTCAAGGCAATGCATTATCATGATGATTTTCGAATAGATGAAGATGGCGATTGGATGCTTCAGGGATGGAAAGGCAGAATCATCTTCGGTCTTGCTTTCTGGAAACCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

543

Amino Acids

61.97

Weight (kDa)

9.04

Isoelectric Point (pI)

46.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	170 - 540	2.2e-121	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca	FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica	MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica	Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis	pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis	RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata	RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora	Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii	Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa	Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha	Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana	Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1462
Acc36I	ACCTGC	2 cut(s)	1432, 1462
Acc65I	GGTACC	1 cut(s)	141
AccB1I	GGYRCC	2 cut(s)	141, 1474
AccI	GTMKAC	2 cut(s)	700, 916
AccII	CGCG	3 cut(s)	655, 1326, 1391
AccIII	TCCGGA	1 cut(s)	1201
AciI	CCGC	3 cut(s)	186, 714, 1339
AclWI	GGATC	1 cut(s)	1080
AcsI	RAATTY	2 cut(s)	70, 181
AcuI	CTGAAG	3 cut(s)	18, 593, 1559
AcyI	GRCGYC	1 cut(s)	985
AfaI	GTAC	2 cut(s)	143, 1031
AfiI	CCNNNNNNNGG	2 cut(s)	947, 1069
AhdI	GACNNNNNGTC	1 cut(s)	984
AjnI	CCWGG	3 cut(s)	486, 946, 1485
AjuI	GAANNNNNNNTTGG	2 cut(s)	936, 968
AloI	GAACNNNNNNTCC	2 cut(s)	87, 119
AluBI	AGCT	7 cut(s)	302, 543, 626, 809, 937, 1096, 1492
AluI	AGCT	7 cut(s)	302, 543, 626, 809, 937, 1096, 1492
Alw21I	GWGCWC	2 cut(s)	529, 1494
Alw26I	GTCTC	3 cut(s)	971, 1049, 1418
Alw44I	GTGCAC	1 cut(s)	525
AlwI	GGATC	1 cut(s)	1080
AlwNI	CAGNNNCTG	1 cut(s)	716
Ama87I	CYCGRG	2 cut(s)	157, 1421
Aor13HI	TCCGGA	1 cut(s)	1201
AoxI	GGCC	5 cut(s)	531, 643, 866, 1311, 1418
ApaLI	GTGCAC	1 cut(s)	525
ApeKI	GCWGC	5 cut(s)	5, 302, 427, 1004, 1336
ApoI	RAATTY	2 cut(s)	70, 181
ArsI	GACNNNNNNTTYG	2 cut(s)	8, 40
AseI	ATTAAT	1 cut(s)	1197
Asp718I	GGTACC	1 cut(s)	141
AspLEI	GCGC	2 cut(s)	655, 657
AspS9I	GGNCC	4 cut(s)	905, 1168, 1204, 1419
AsuC2I	CCSGG	2 cut(s)	158, 159
AsuHPI	GGTGA	5 cut(s)	275, 617, 678, 1165, 1236
AsuII	TTCGAA	1 cut(s)	1546
AvaI	CYCGRG	2 cut(s)	157, 1421
AvaII	GGWCC	3 cut(s)	905, 1168, 1204
BaeGI	GKGCMC	1 cut(s)	529
BanI	GGYRCC	2 cut(s)	141, 1474
BanII	GRGCYC	1 cut(s)	1494
Bbv12I	GWGCWC	2 cut(s)	529, 1494
BbvI	GCAGC	4 cut(s)	289, 439, 991, 1348
BccI	CCATC	7 cut(s)	200, 254, 269, 635, 685, 1553, 1575
BceAI	ACGGC	2 cut(s)	552, 1462
BciT130I	CCWGG	3 cut(s)	488, 948, 1487
BclI	TGATCA	1 cut(s)	1185
BcnI	CCSGG	2 cut(s)	158, 159
BcoDI	GTCTC	3 cut(s)	971, 1049, 1418
BfaI	CTAG	3 cut(s)	647, 806, 912
BfuAI	ACCTGC	2 cut(s)	1432, 1462
BisI	GCNGC	6 cut(s)	6, 303, 428, 714, 1005, 1337
BlsI	GCNGC	6 cut(s)	7, 304, 429, 715, 1006, 1338
Bme1390I	CCNGG	5 cut(s)	158, 159, 488, 948, 1487
Bme18I	GGWCC	3 cut(s)	905, 1168, 1204
BmeRI	GACNNNNNGTC	1 cut(s)	984
BmeT110I	CYCGRG	2 cut(s)	157, 1421
BmgT120I	GGNCC	4 cut(s)	905, 1168, 1204, 1419
BmiI	GGNNCC	4 cut(s)	143, 667, 1169, 1476
BmrFI	CCNGG	5 cut(s)	158, 159, 488, 948, 1487
BmsI	GCATC	3 cut(s)	598, 1126, 1560
BplI	GAGNNNNNCTC	2 cut(s)	610, 642
Bpu10I	CCTNAGC	1 cut(s)	9
Bpu14I	TTCGAA	1 cut(s)	1546
BpuEI	CTTGAG	2 cut(s)	1088, 1502
BpuMI	CCSGG	2 cut(s)	158, 159
BsaHI	GRCGYC	1 cut(s)	985
BsaJI	CCNNGG	5 cut(s)	156, 157, 534, 1146, 1163
BsaWI	WCCGGW	1 cut(s)	1201
Bsc4I	CCNNNNNNNGG	2 cut(s)	947, 1069
Bse1I	ACTGG	2 cut(s)	1021, 1407
Bse3DI	GCAATG	1 cut(s)	1530
BseAI	TCCGGA	1 cut(s)	1201
BseBI	CCWGG	3 cut(s)	488, 948, 1487
BseDI	CCNNGG	5 cut(s)	156, 157, 534, 1146, 1163
BseGI	GGATG	4 cut(s)	265, 1347, 1575, 1586
BseLI	CCNNNNNNNGG	2 cut(s)	947, 1069
BseMI	GCAATG	1 cut(s)	1530
BseMII	CTCAG	5 cut(s)	33, 164, 231, 312, 686
BseNI	ACTGG	2 cut(s)	1021, 1407
BsePI	GCGCGC	1 cut(s)	653
BseRI	GAGGAG	3 cut(s)	93, 190, 989
BseSI	GKGCMC	1 cut(s)	529
BseXI	GCAGC	4 cut(s)	289, 439, 991, 1348
BseYI	CCCAGC	1 cut(s)	1460
Bsh1236I	CGCG	3 cut(s)	655, 1326, 1391
Bsh1285I	CGRYCG	1 cut(s)	959
BshFI	GGCC	5 cut(s)	533, 645, 868, 1313, 1420
BshNI	GGYRCC	2 cut(s)	141, 1474
BsiEI	CGRYCG	1 cut(s)	959
BsiHKAI	GWGCWC	2 cut(s)	529, 1494
BsiHKCI	CYCGRG	2 cut(s)	157, 1421
BsiSI	CCGG	2 cut(s)	158, 1202
BslFI	GGGAC	2 cut(s)	1181, 1246
BslI	CCNNNNNNNGG	2 cut(s)	947, 1069
BsmAI	GTCTC	3 cut(s)	971, 1049, 1418
BsmBI	CGTCTC	1 cut(s)	971
BsmFI	GGGAC	2 cut(s)	1181, 1246
BsnI	GGCC	5 cut(s)	533, 645, 868, 1313, 1420
BsoBI	CYCGRG	2 cut(s)	157, 1421
Bsp119I	TTCGAA	1 cut(s)	1546
Bsp1286I	GDGCHC	2 cut(s)	529, 1494
Bsp13I	TCCGGA	1 cut(s)	1201
Bsp143I	GATC	2 cut(s)	1072, 1185
Bsp68I	TCGCGA	1 cut(s)	1326
BspACI	CCGC	3 cut(s)	186, 714, 1339
BspANI	GGCC	5 cut(s)	533, 645, 868, 1313, 1420
BspCNI	CTCAG	5 cut(s)	32, 165, 230, 313, 687
BspEI	TCCGGA	1 cut(s)	1201
BspFNI	CGCG	3 cut(s)	655, 1326, 1391
BspHI	TCATGA	1 cut(s)	1534
BspLI	GGNNCC	4 cut(s)	143, 667, 1169, 1476
BspMI	ACCTGC	2 cut(s)	1432, 1462
BspPI	GGATC	1 cut(s)	1080
BspT104I	TTCGAA	1 cut(s)	1546
BspT107I	GGYRCC	2 cut(s)	141, 1474
BsrDI	GCAATG	1 cut(s)	1530
BsrI	ACTGG	2 cut(s)	1021, 1407
BssECI	CCNNGG	5 cut(s)	156, 157, 534, 1146, 1163
BssHII	GCGCGC	1 cut(s)	653
BssMI	GATC	2 cut(s)	1072, 1185
BssNAI	GTATAC	2 cut(s)	701, 917
BssNI	GRCGYC	1 cut(s)	985
Bst1107I	GTATAC	2 cut(s)	701, 917
Bst2UI	CCWGG	3 cut(s)	488, 948, 1487
Bst4CI	ACNGT	4 cut(s)	441, 1102, 1147, 1263
Bst6I	CTCTTC	2 cut(s)	598, 696
BstACI	GRCGYC	1 cut(s)	985
BstBI	TTCGAA	1 cut(s)	1546
BstC8I	GCNNGC	4 cut(s)	375, 541, 655, 1115
BstDEI	CTNAG	7 cut(s)	9, 19, 173, 217, 321, 695, 1626
BstDSI	CCRYGG	2 cut(s)	534, 1146
BstF5I	GGATG	4 cut(s)	265, 1347, 1575, 1586
BstFNI	CGCG	3 cut(s)	655, 1326, 1391
BstHHI	GCGC	2 cut(s)	655, 657
BstKTI	GATC	2 cut(s)	1075, 1188
BstMAI	GTCTC	3 cut(s)	971, 1049, 1418
BstMBI	GATC	2 cut(s)	1072, 1185
BstMCI	CGRYCG	1 cut(s)	959
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 815
BstNI	CCWGG	3 cut(s)	488, 948, 1487
BstNSI	RCATGY	2 cut(s)	377, 1117
BstSCI	CCNGG	5 cut(s)	156, 157, 486, 946, 1485
BstSLI	GKGCMC	1 cut(s)	529
BstUI	CGCG	3 cut(s)	655, 1326, 1391
BstV1I	GCAGC	4 cut(s)	289, 439, 991, 1348
BstX2I	RGATCY	1 cut(s)	1072
BstYI	RGATCY	1 cut(s)	1072
BstZ17I	GTATAC	2 cut(s)	701, 917
BsuRI	GGCC	5 cut(s)	533, 645, 868, 1313, 1420
BtgI	CCRYGG	2 cut(s)	534, 1146
BtsCI	GGATG	4 cut(s)	265, 1347, 1575, 1586
BtsI	GCAGTG	2 cut(s)	412, 1442
BtsIMutI	CAGTG	4 cut(s)	170, 318, 412, 1442
BtuMI	TCGCGA	1 cut(s)	1326
BveI	ACCTGC	2 cut(s)	1432, 1462
Cac8I	GCNNGC	4 cut(s)	375, 541, 655, 1115
CaiI	CAGNNNCTG	1 cut(s)	716
CciI	TCATGA	1 cut(s)	1534
CfoI	GCGC	2 cut(s)	655, 657
Cfr13I	GGNCC	4 cut(s)	905, 1168, 1204, 1419
Cfr9I	CCCGGG	1 cut(s)	157
CseI	GACGC	2 cut(s)	366, 974
CsiI	ACCWGGT	1 cut(s)	946
Csp6I	GTAC	2 cut(s)	142, 1030
CviAII	CATG	5 cut(s)	119, 359, 374, 1114, 1535
CviQI	GTAC	2 cut(s)	142, 1030
DdeI	CTNAG	7 cut(s)	9, 19, 173, 217, 321, 695, 1626
DpnI	GATC	2 cut(s)	1074, 1187
DpnII	GATC	2 cut(s)	1072, 1185
DriI	GACNNNNNGTC	1 cut(s)	984
Eam1104I	CTCTTC	2 cut(s)	598, 696
Eam1105I	GACNNNNNGTC	1 cut(s)	984
EarI	CTCTTC	2 cut(s)	598, 696
Ecl136II	GAGCTC	1 cut(s)	1492
Eco24I	GRGCYC	1 cut(s)	1494
Eco32I	GATATC	1 cut(s)	1451
Eco47I	GGWCC	3 cut(s)	905, 1168, 1204
Eco53kI	GAGCTC	1 cut(s)	1492
Eco57I	CTGAAG	3 cut(s)	18, 593, 1559
Eco88I	CYCGRG	2 cut(s)	157, 1421
EcoICRI	GAGCTC	1 cut(s)	1492
EcoO109I	RGGNCCY	1 cut(s)	1168
EcoRI	GAATTC	1 cut(s)	181
EcoRII	CCWGG	3 cut(s)	486, 946, 1485
EcoRV	GATATC	1 cut(s)	1451
EcoT22I	ATGCAT	2 cut(s)	1119, 1530
EcoT38I	GRGCYC	1 cut(s)	1494
Esp3I	CGTCTC	1 cut(s)	971
FaeI	CATG	5 cut(s)	122, 362, 377, 1117, 1538
FaqI	GGGAC	2 cut(s)	1181, 1246
FatI	CATG	5 cut(s)	118, 358, 373, 1113, 1534
FauNDI	CATATG	1 cut(s)	768
FbaI	TGATCA	1 cut(s)	1185
FblI	GTMKAC	2 cut(s)	700, 916
Fnu4HI	GCNGC	6 cut(s)	6, 303, 428, 714, 1005, 1337
FokI	GGATG	4 cut(s)	272, 1354, 1582, 1593
FriOI	GRGCYC	1 cut(s)	1494
Fsp4HI	GCNGC	6 cut(s)	6, 303, 428, 714, 1005, 1337
FspBI	CTAG	3 cut(s)	647, 806, 912
GlaI	GCGC	2 cut(s)	654, 656
GluI	GCNGC	6 cut(s)	6, 303, 428, 714, 1005, 1337
GsaI	CCCAGC	1 cut(s)	1464
HaeIII	GGCC	5 cut(s)	533, 645, 868, 1313, 1420
HapII	CCGG	2 cut(s)	158, 1202
HgaI	GACGC	2 cut(s)	366, 974
HhaI	GCGC	2 cut(s)	655, 657
Hin1I	GRCGYC	1 cut(s)	985
Hin1II	CATG	5 cut(s)	122, 362, 377, 1117, 1538
Hin6I	GCGC	2 cut(s)	653, 655
HinP1I	GCGC	2 cut(s)	653, 655
HincII	GTYRAC	2 cut(s)	1108, 1228
HindII	GTYRAC	2 cut(s)	1108, 1228
HinfI	GANTC	7 cut(s)	17, 29, 123, 244, 364, 672, 1593
HpaI	GTTAAC	1 cut(s)	1108
HpaII	CCGG	2 cut(s)	158, 1202
HphI	GGTGA	5 cut(s)	275, 617, 678, 1165, 1236
Hpy166II	GTNNAC	7 cut(s)	527, 566, 701, 905, 917, 1108, 1228
Hpy188I	TCNGA	5 cut(s)	37, 696, 892, 1164, 1190
Hpy188III	TCNNGA	6 cut(s)	389, 1202, 1325, 1495, 1535, 1615
Hpy8I	GTNNAC	7 cut(s)	527, 566, 701, 905, 917, 1108, 1228
Hpy99I	CGWCG	3 cut(s)	317, 990, 1391
HpyAV	CCTTC	4 cut(s)	226, 246, 1258, 1391
HpyCH4III	ACNGT	4 cut(s)	441, 1102, 1147, 1263
HpyCH4IV	ACGT	1 cut(s)	919
HpyCH4V	TGCA	9 cut(s)	272, 427, 527, 611, 1007, 1043, 1113, 1117, 1528
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 815
HpyF3I	CTNAG	7 cut(s)	9, 19, 173, 217, 321, 695, 1626
HpySE526I	ACGT	1 cut(s)	919
Hsp92I	GRCGYC	1 cut(s)	985
Hsp92II	CATG	5 cut(s)	122, 362, 377, 1117, 1538
HspAI	GCGC	2 cut(s)	653, 655
Kpn2I	TCCGGA	1 cut(s)	1201
KpnI	GGTACC	1 cut(s)	145
Ksp22I	TGATCA	1 cut(s)	1185
KspAI	GTTAAC	1 cut(s)	1108
Kzo9I	GATC	2 cut(s)	1072, 1185
LmnI	GCTCC	2 cut(s)	665, 1489
Lsp1109I	GCAGC	4 cut(s)	289, 439, 991, 1348
LweI	GCATC	3 cut(s)	598, 1126, 1560
MabI	ACCWGGT	1 cut(s)	946
MaeI	CTAG	3 cut(s)	647, 806, 912
MaeII	ACGT	1 cut(s)	919
MaeIII	GTNAC	3 cut(s)	389, 771, 1255
MalI	GATC	2 cut(s)	1074, 1187
MboI	GATC	2 cut(s)	1072, 1185
MfeI	CAATTG	1 cut(s)	930
MflI	RGATCY	1 cut(s)	1072
MhlI	GDGCHC	2 cut(s)	529, 1494
MluCI	AATT	9 cut(s)	70, 146, 181, 748, 795, 930, 1080, 1194, 1240
MlyI	GAGTC	1 cut(s)	11
MmeI	TCCRAC	3 cut(s)	810, 904, 1087
Mph1103I	ATGCAT	2 cut(s)	1119, 1530
MroI	TCCGGA	1 cut(s)	1201
MseI	TTAA	8 cut(s)	747, 801, 1014, 1077, 1107, 1124, 1197, 1481
MslI	CAYNNNNRTG	3 cut(s)	372, 1048, 1533
MspA1I	CMGCKG	3 cut(s)	302, 716, 1339
MspI	CCGG	2 cut(s)	158, 1202
MspR9I	CCNGG	5 cut(s)	158, 159, 488, 948, 1487
MunI	CAATTG	1 cut(s)	930
MvaI	CCWGG	3 cut(s)	488, 948, 1487
MvnI	CGCG	3 cut(s)	655, 1326, 1391
MwoI	GCNNNNNNNGC	2 cut(s)	11, 815
NciI	CCSGG	2 cut(s)	158, 159
NdeI	CATATG	1 cut(s)	768
NdeII	GATC	2 cut(s)	1072, 1185
NlaIII	CATG	5 cut(s)	122, 362, 377, 1117, 1538
NlaIV	GGNNCC	4 cut(s)	143, 667, 1169, 1476
NmuCI	GTSAC	2 cut(s)	389, 1255
NruI	TCGCGA	1 cut(s)	1326
NsiI	ATGCAT	2 cut(s)	1119, 1530
NspI	RCATGY	2 cut(s)	377, 1117
NspV	TTCGAA	1 cut(s)	1546
PaeI	GCATGC	2 cut(s)	377, 1117
PagI	TCATGA	1 cut(s)	1534
PaqCI	CACCTGC	1 cut(s)	1462
PauI	GCGCGC	1 cut(s)	653
PfeI	GAWTC	6 cut(s)	29, 123, 244, 364, 672, 1593
PflFI	GACNNNGTC	1 cut(s)	1172
PkrI	GCNGC	6 cut(s)	7, 304, 429, 715, 1006, 1338
PleI	GAGTC	1 cut(s)	11
PpsI	GAGTC	1 cut(s)	11
PpuMI	RGGWCCY	1 cut(s)	1168
PshBI	ATTAAT	1 cut(s)	1197
Psp124BI	GAGCTC	1 cut(s)	1494
Psp5II	RGGWCCY	1 cut(s)	1168
Psp6I	CCWGG	3 cut(s)	486, 946, 1485
PspFI	CCCAGC	1 cut(s)	1460
PspGI	CCWGG	3 cut(s)	486, 946, 1485
PspN4I	GGNNCC	4 cut(s)	143, 667, 1169, 1476
PspPI	GGNCC	4 cut(s)	905, 1168, 1204, 1419
PspPPI	RGGWCCY	1 cut(s)	1168
PstNI	CAGNNNCTG	1 cut(s)	716
PsuI	RGATCY	1 cut(s)	1072
PsyI	GACNNNGTC	1 cut(s)	1172
PteI	GCGCGC	1 cut(s)	653
PvuII	CAGCTG	1 cut(s)	302
RruI	TCGCGA	1 cut(s)	1326
RsaI	GTAC	2 cut(s)	143, 1031
RsaNI	GTAC	2 cut(s)	142, 1030
RseI	CAYNNNNRTG	3 cut(s)	372, 1048, 1533
SacI	GAGCTC	1 cut(s)	1494
SaqAI	TTAA	8 cut(s)	747, 801, 1014, 1077, 1107, 1124, 1197, 1481
SatI	GCNGC	6 cut(s)	6, 303, 428, 714, 1005, 1337
Sau3AI	GATC	2 cut(s)	1072, 1185
Sau96I	GGNCC	4 cut(s)	905, 1168, 1204, 1419
SchI	GAGTC	1 cut(s)	11
ScrFI	CCNGG	5 cut(s)	158, 159, 488, 948, 1487
SduI	GDGCHC	2 cut(s)	529, 1494
SexAI	ACCWGGT	1 cut(s)	946
SfaNI	GCATC	3 cut(s)	598, 1126, 1560
SfuI	TTCGAA	1 cut(s)	1546
SinI	GGWCC	3 cut(s)	905, 1168, 1204
SmaI	CCCGGG	1 cut(s)	159
SmiMI	CAYNNNNRTG	3 cut(s)	372, 1048, 1533
SmlI	CTYRAG	2 cut(s)	1067, 1517
SmoI	CTYRAG	2 cut(s)	1067, 1517
SphI	GCATGC	2 cut(s)	377, 1117
Sse9I	AATT	9 cut(s)	70, 146, 181, 748, 795, 930, 1080, 1194, 1240
SsiI	CCGC	3 cut(s)	186, 714, 1339
SspMI	CTAG	3 cut(s)	647, 806, 912
SstI	GAGCTC	1 cut(s)	1494
StyD4I	CCNGG	5 cut(s)	156, 157, 486, 946, 1485
TaaI	ACNGT	4 cut(s)	441, 1102, 1147, 1263
TaiI	ACGT	1 cut(s)	922
TaqI	TCGA	4 cut(s)	27, 250, 955, 1546
TaqII	GACCGA	2 cut(s)	973, 1592
TasI	AATT	9 cut(s)	70, 146, 181, 748, 795, 930, 1080, 1194, 1240
TatI	WGTACW	1 cut(s)	1029
TauI	GCSGC	1 cut(s)	716
TfiI	GAWTC	6 cut(s)	29, 123, 244, 364, 672, 1593
Tru1I	TTAA	8 cut(s)	747, 801, 1014, 1077, 1107, 1124, 1197, 1481
Tru9I	TTAA	8 cut(s)	747, 801, 1014, 1077, 1107, 1124, 1197, 1481
TscAI	CASTG	4 cut(s)	177, 325, 412, 1442
TseFI	GTSAC	2 cut(s)	389, 1255
TseI	GCWGC	5 cut(s)	5, 302, 427, 1004, 1336
Tsp45I	GTSAC	2 cut(s)	389, 1255
TspDTI	ATGAA	4 cut(s)	1155, 1201, 1394, 1569
TspGWI	ACGGA	1 cut(s)	1233
TspMI	CCCGGG	1 cut(s)	157
TspRI	CASTG	4 cut(s)	177, 325, 412, 1442
Tth111I	GACNNNGTC	1 cut(s)	1172
VneI	GTGCAC	1 cut(s)	525
VpaK11BI	GGWCC	3 cut(s)	905, 1168, 1204
VspI	ATTAAT	1 cut(s)	1197
XapI	RAATTY	2 cut(s)	70, 181
XceI	RCATGY	2 cut(s)	377, 1117
XmaI	CCCGGG	1 cut(s)	157
XmiI	GTMKAC	2 cut(s)	700, 916
XspI	CTAG	3 cut(s)	647, 806, 912
Zsp2I	ATGCAT	2 cut(s)	1119, 1530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.