RchiOBHm_Chr5g0065601

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000175
Physical Location & Seq
Forward (+)
71591994 .. 71595127
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34146

Sequence Viewer

Length: 2073 bp
ATGGAGAGGTTCAAATTTGACCATGGCCCAGTTTCAGTCTGTCCAAATCCCAATCTTGTAAATGTAAATGGGTTCAAAATAAATCATGAATCCACCAATCCAGTATTACTTGCATCCAAATCTGATCTTTCTAGTGATTCAAGTAATGAGGGTTCAGAGGTAGATGGTCTTGATATCAATGATTGCAACCATCCTGTACTCAAGTACATAAGTGATATTCTTCTAGAAGAAGACTTGGAGGGTAAGCCCTGCATGTTGCAGGACTGTTTAGCCCTCCAAGCTGCTGAGAAGTCTTTCTATGATGTCCTTAATCAGAAGGACCCTCCTTTACCCAACCAACCCTCTAGTTTTGTGCACCAAAGCTCTGAGAGTTCAGATGATGATTCCCCACAGAGTTTCCGAAACAGTAATGGCTCTTTTGCTGTTAAGACAGATTGGGTTTGGGATCCGGAATGCTCTCCCGTCCAATCTTCTCTATTTGAGTCTTGGTTGAATACCCGTTTGGTTTCTGATTCATTGTCTGAGATGCAAAGTCTTGGGAACTTTGGAGGTGTGGGGGAAGCAAGCAAGTTCCTTTCAAATCCGAAACTTGAAATGTTTGACTTAGAGAGGTTTCAATTGATGCCTCAAGGTTCAGTCGCATCTGAGTCAGGGGCTGAAGGCTACAACTCGACCAATGGATCAAAGGGAAAGAAGACTCATCAAAGGGAGGATAGTCATTTTCCAGAAGAAGAGAGAAGCAACAAGCAGTCTGCTGCTGTAGGGGATGACTTTGAGCCACAAGAAATGTTTGATGAGGTTTTGCTTTGTGCTGAAAATCATGATGCTATGCTTTGTGCTGATTATGAGTCTTTGAAAACTGAAGGAGATGGGAAGTTGCAGCGTAACAAGCAGTCAAAAGGATTGAAGGCAAAGCGACAGAGTGACATTGGCGAAGTGGTAGATTTGTGCACAATGCTAACTCAATGTGCACAAGCTGTGGCGAACTTTGACCAACTAACTGCAAGTGAACTACTCAAGCAGATAAGGCAGCACACAACCCCCCATGGTGATGCAACCCAGAGGTTAGCCCATTACTTTGCTGATGGCCTCCACGCGCGCTTGGCTGGTGCCAAAACCCCATCGTATTCGCCTCTTGTAAGTGTGCAGACATCAGCTGCTGAAATCTTAAAAGCATACCAGGTAGTTATTACAGCATGCCCTTTCAAGAAGATGTCACATTTCTTTGCCAACAGAACAATCATGAAGCTAACAGAAAAAGCGACAAGGCTTCACATTATTGATTTTGGTATTTCTTATGGATTTCAATGGCCTTGCCTGATCCAACGTCTGTCCCAAAGACCTGGTGGACCTCCTAAGCTTCGTATCACAGCCATTGAGCTTCCCCAACCAGGTTTTCGACCTACAGAAAGAGTTGAAGAGACTGGACGTCGCTTAGAAAAGTATGCCAAGAGATTTGATGTCCCATTTGAGTATAATGTCATTGCCCAGAAATGGGAAACTATCCGAATTGAGGACATCAAAATTGACCGAACTGAGCTGATTGTGGTCAATTGTTTGCACCGGTTAAGGAACATACCAGATGAGACGGTGATGGTGAACAGTCCAAGAGATGCTGTCCTTAAGTTGATAAGGAAAATTAGCCCGGACATATTCATTCACGGTGTTGTCAATGGGACATACAATGCTCCCTTCTTTCTCACACGATTTAGAGAGGCACTCCTCCACTTCTATTCACTGTTTGATATGCTTGAGGCGACCATCTCTCGTGAAGATGAACAGCGGCTGATGTTTGAACAAGCAATATATGGTAGAGATATAATTAATGTCGTAGCGTGTGAAGGACTGGAAAGAGTTGAAAGGCCTGAAACATACAGGCAGTGGCAGGTCAGGAATTTGAGGGCTGGGTTCAAGCAGCTACCATTAGACCAGAAGCTGTTGAAGGAAGTGAAGACTATGTCAAAAGTAATGTGTTATCATAATGATTTTAGAGTTGATGAAGATGGCCATTGGATGCTGCAGGGATGGAAAGGAAGAACCATCATGGCTCTTTCTTTTTGGAAGCCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

690

Amino Acids

78.27

Weight (kDa)

5.67

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 318 - 688 1.4e-121 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1432
Acc36I ACCTGC 1 cut(s) 1876
AccB1I GGYRCC 1 cut(s) 1109
AccII CGCG 2 cut(s) 1097, 1099
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 1 cut(s) 1783
AclWI GGATC 4 cut(s) 440, 453, 688, 1315
AcoI YGGCCR 1 cut(s) 2005
AcsI RAATTY 2 cut(s) 14, 1894
AcuI CTGAAG 2 cut(s) 678, 882
AcyI GRCGYC 1 cut(s) 1429
AfaI GTAC 2 cut(s) 198, 206
AfiI CCNNNNNNNGG 4 cut(s) 1391, 1494, 1495, 1513
AflII CTTAAG 1 cut(s) 1622
AgeI ACCGGT 1 cut(s) 1563
AhdI GACNNNNNGTC 1 cut(s) 1428
AjnI CCWGG 3 cut(s) 1179, 1342, 1390
AjuI GAANNNNNNNTTGG 4 cut(s) 485, 517, 1380, 1412
Alw21I GWGCWC 3 cut(s) 357, 953, 973
Alw26I GTCTC 2 cut(s) 1415, 1580
Alw44I GTGCAC 3 cut(s) 353, 949, 969
AlwI GGATC 4 cut(s) 440, 453, 688, 1315
AlwNI CAGNNNCTG 4 cut(s) 656, 1160, 1786, 1936
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 5 cut(s) 25, 1087, 1310, 1862, 2005
ApaLI GTGCAC 3 cut(s) 353, 949, 969
ApeKI GCWGC 7 cut(s) 281, 755, 880, 1030, 1157, 1915, 2017
ApoI RAATTY 2 cut(s) 14, 1894
AseI ATTAAT 1 cut(s) 1824
AsiGI ACCGGT 1 cut(s) 1563
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 2 cut(s) 1099, 1101
AspS9I GGNCC 3 cut(s) 26, 319, 1349
AsuC2I CCSGG 1 cut(s) 1646
AsuHPI GGTGA 3 cut(s) 1061, 1603, 1609
AvaII GGWCC 2 cut(s) 319, 1349
BaeGI GKGCMC 3 cut(s) 357, 953, 973
BalI TGGCCA 1 cut(s) 2007
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 1109
BauI CACGAG 1 cut(s) 1767
BbsI GAAGAC 3 cut(s) 237, 701, 1958
Bbv12I GWGCWC 3 cut(s) 357, 953, 973
BbvI GCAGC 7 cut(s) 268, 742, 892, 1042, 1144, 1927, 2004
BciT130I CCWGG 3 cut(s) 1181, 1344, 1392
BcnI CCSGG 1 cut(s) 1646
BcoDI GTCTC 2 cut(s) 1415, 1580
BfaI CTAG 5 cut(s) 132, 224, 345, 2067, 2071
BfmI CTRYAG 3 cut(s) 759, 1404, 2018
BfrI CTTAAG 1 cut(s) 1622
BfuAI ACCTGC 1 cut(s) 1876
BisI GCNGC 8 cut(s) 282, 756, 881, 1031, 1158, 1784, 1916, 2018
BlsI GCNGC 8 cut(s) 283, 757, 882, 1032, 1159, 1785, 1917, 2019
Bme1390I CCNGG 4 cut(s) 1181, 1344, 1392, 1646
Bme18I GGWCC 2 cut(s) 319, 1349
BmeRI GACNNNNNGTC 1 cut(s) 1428
BmgT120I GGNCC 3 cut(s) 26, 319, 1349
BmiI GGNNCC 3 cut(s) 321, 447, 1111
BmrFI CCNGG 4 cut(s) 1181, 1344, 1392, 1646
BmrI ACTGGG 1 cut(s) 23
BmsI GCATC 8 cut(s) 122, 516, 612, 650, 814, 1042, 1603, 2004
BmuI ACTGGG 1 cut(s) 23
BpiI GAAGAC 3 cut(s) 237, 701, 1958
BplI GAGNNNNNCTC 2 cut(s) 1704, 1736
Bpu10I CCTNAGC 1 cut(s) 1356
BpuEI CTTGAG 4 cut(s) 185, 612, 1001, 1772
BpuMI CCSGG 1 cut(s) 1646
BsaHI GRCGYC 1 cut(s) 1429
BsaJI CCNNGG 2 cut(s) 22, 1045
BsaWI WCCGGW 2 cut(s) 448, 1563
Bsc4I CCNNNNNNNGG 4 cut(s) 1391, 1494, 1495, 1513
Bse118I RCCGGY 1 cut(s) 1563
Bse1I ACTGG 4 cut(s) 29, 101, 1429, 1851
Bse3DI GCAATG 1 cut(s) 1482
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 3 cut(s) 1181, 1344, 1392
BseDI CCNNGG 2 cut(s) 22, 1045
BseGI GGATG 5 cut(s) 113, 190, 772, 2019, 2030
BseLI CCNNNNNNNGG 4 cut(s) 1391, 1494, 1495, 1513
BseMI GCAATG 1 cut(s) 1482
BseMII CTCAG 5 cut(s) 276, 357, 513, 636, 1527
BseNI ACTGG 4 cut(s) 29, 101, 1429, 1851
BsePI GCGCGC 1 cut(s) 1097
BseRI GAGGAG 1 cut(s) 1712
BseSI GKGCMC 3 cut(s) 357, 953, 973
BseXI GCAGC 7 cut(s) 268, 742, 892, 1042, 1144, 1927, 2004
BseYI CCCAGC 1 cut(s) 1904
BsgI GTGCAG 1 cut(s) 1166
Bsh1236I CGCG 2 cut(s) 1097, 1099
BshFI GGCC 5 cut(s) 27, 1089, 1312, 1864, 2007
BshNI GGYRCC 1 cut(s) 1109
BshTI ACCGGT 1 cut(s) 1563
BsiHKAI GWGCWC 3 cut(s) 357, 953, 973
BsiSI CCGG 3 cut(s) 449, 1564, 1646
BslFI GGGAC 3 cut(s) 1318, 1448, 1690
BslI CCNNNNNNNGG 4 cut(s) 1391, 1494, 1495, 1513
BsmAI GTCTC 2 cut(s) 1415, 1580
BsmBI CGTCTC 1 cut(s) 1580
BsmFI GGGAC 3 cut(s) 1318, 1448, 1690
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 5 cut(s) 27, 1089, 1312, 1864, 2007
Bsp1286I GDGCHC 3 cut(s) 357, 953, 973
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 4 cut(s) 124, 445, 680, 1320
Bsp19I CCATGG 2 cut(s) 22, 1045
BspACI CCGC 1 cut(s) 1783
BspANI GGCC 5 cut(s) 27, 1089, 1312, 1864, 2007
BspCNI CTCAG 5 cut(s) 277, 358, 514, 637, 1528
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 2 cut(s) 1097, 1099
BspHI TCATGA 3 cut(s) 85, 820, 1242
BspLI GGNNCC 3 cut(s) 321, 447, 1111
BspMAI CTGCAG 1 cut(s) 2022
BspMI ACCTGC 1 cut(s) 1876
BspPI GGATC 4 cut(s) 440, 453, 688, 1315
BspT107I GGYRCC 1 cut(s) 1109
BspTI CTTAAG 1 cut(s) 1622
BsrDI GCAATG 1 cut(s) 1482
BsrFI RCCGGY 1 cut(s) 1563
BsrI ACTGG 4 cut(s) 29, 101, 1429, 1851
BssAI RCCGGY 1 cut(s) 1563
BssECI CCNNGG 2 cut(s) 22, 1045
BssHII GCGCGC 1 cut(s) 1097
BssMI GATC 4 cut(s) 124, 445, 680, 1320
BssNI GRCGYC 1 cut(s) 1429
BssSI CACGAG 1 cut(s) 1767
BssT1I CCWWGG 2 cut(s) 22, 1045
Bst2BI CACGAG 1 cut(s) 1767
Bst2UI CCWGG 3 cut(s) 1181, 1344, 1392
Bst4CI ACNGT 6 cut(s) 266, 407, 1591, 1604, 1664, 1740
Bst6I CTCTTC 2 cut(s) 726, 1413
BstACI GRCGYC 1 cut(s) 1429
BstAFI CTTAAG 1 cut(s) 1622
BstC8I GCNNGC 3 cut(s) 565, 1099, 1198
BstDEI CTNAG 8 cut(s) 285, 366, 522, 604, 645, 1356, 1435, 1536
BstDSI CCRYGG 2 cut(s) 22, 1045
BstF5I GGATG 5 cut(s) 113, 190, 772, 2019, 2030
BstFNI CGCG 2 cut(s) 1097, 1099
BstHHI GCGC 2 cut(s) 1099, 1101
BstKTI GATC 4 cut(s) 127, 448, 683, 1323
BstMAI GTCTC 2 cut(s) 1415, 1580
BstMBI GATC 4 cut(s) 124, 445, 680, 1320
BstMWI GCNNNNNNNGC 4 cut(s) 278, 889, 1027, 1103
BstNI CCWGG 3 cut(s) 1181, 1344, 1392
BstNSI RCATGY 2 cut(s) 256, 1200
BstSCI CCNGG 4 cut(s) 1179, 1342, 1390, 1644
BstSFI CTRYAG 3 cut(s) 759, 1404, 2018
BstSLI GKGCMC 3 cut(s) 357, 953, 973
BstUI CGCG 2 cut(s) 1097, 1099
BstV1I GCAGC 7 cut(s) 268, 742, 892, 1042, 1144, 1927, 2004
BstV2I GAAGAC 3 cut(s) 237, 701, 1958
BstX2I RGATCY 1 cut(s) 445
BstXI CCANNNNNNTGG 1 cut(s) 1343
BstYI RGATCY 1 cut(s) 445
BsuRI GGCC 5 cut(s) 27, 1089, 1312, 1864, 2007
BtgI CCRYGG 2 cut(s) 22, 1045
BtsCI GGATG 5 cut(s) 113, 190, 772, 2019, 2030
BtsI GCAGTG 1 cut(s) 1886
BtsIMutI CAGTG 2 cut(s) 1736, 1886
BveI ACCTGC 1 cut(s) 1876
Cac8I GCNNGC 3 cut(s) 565, 1099, 1198
CaiI CAGNNNCTG 4 cut(s) 656, 1160, 1786, 1936
CciI TCATGA 3 cut(s) 85, 820, 1242
CfoI GCGC 2 cut(s) 1099, 1101
Cfr10I RCCGGY 1 cut(s) 1563
Cfr13I GGNCC 3 cut(s) 26, 319, 1349
CsiI ACCWGGT 3 cut(s) 1179, 1342, 1390
Csp6I GTAC 2 cut(s) 197, 205
CspAI ACCGGT 1 cut(s) 1563
CviAII CATG 8 cut(s) 23, 86, 253, 821, 1046, 1197, 1243, 2044
CviQI GTAC 2 cut(s) 197, 205
DdeI CTNAG 8 cut(s) 285, 366, 522, 604, 645, 1356, 1435, 1536
DpnI GATC 4 cut(s) 126, 447, 682, 1322
DpnII GATC 4 cut(s) 124, 445, 680, 1320
DriI GACNNNNNGTC 1 cut(s) 1428
EaeI YGGCCR 1 cut(s) 2005
Eam1104I CTCTTC 2 cut(s) 726, 1413
Eam1105I GACNNNNNGTC 1 cut(s) 1428
EarI CTCTTC 2 cut(s) 726, 1413
Eco130I CCWWGG 2 cut(s) 22, 1045
Eco147I AGGCCT 1 cut(s) 1864
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 2 cut(s) 319, 1349
Eco57I CTGAAG 2 cut(s) 678, 882
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 1179, 1342, 1390
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 22, 1045
ErhI CCWWGG 2 cut(s) 22, 1045
Esp3I CGTCTC 1 cut(s) 1580
FaeI CATG 8 cut(s) 26, 89, 256, 824, 1049, 1200, 1246, 2047
FaqI GGGAC 3 cut(s) 1318, 1448, 1690
FatI CATG 8 cut(s) 22, 85, 252, 820, 1045, 1196, 1242, 2043
Fnu4HI GCNGC 8 cut(s) 282, 756, 881, 1031, 1158, 1784, 1916, 2018
FokI GGATG 5 cut(s) 100, 177, 779, 2026, 2037
Fsp4HI GCNGC 8 cut(s) 282, 756, 881, 1031, 1158, 1784, 1916, 2018
FspBI CTAG 5 cut(s) 132, 224, 345, 2067, 2071
GlaI GCGC 2 cut(s) 1098, 1100
GluI GCNGC 8 cut(s) 282, 756, 881, 1031, 1158, 1784, 1916, 2018
GsaI CCCAGC 1 cut(s) 1908
HaeIII GGCC 5 cut(s) 27, 1089, 1312, 1864, 2007
HapII CCGG 3 cut(s) 449, 1564, 1646
HhaI GCGC 2 cut(s) 1099, 1101
Hin1I GRCGYC 1 cut(s) 1429
Hin1II CATG 8 cut(s) 26, 89, 256, 824, 1049, 1200, 1246, 2047
Hin6I GCGC 2 cut(s) 1097, 1099
HinP1I GCGC 2 cut(s) 1097, 1099
HindIII AAGCTT 1 cut(s) 1358
HinfI GANTC 8 cut(s) 89, 137, 383, 482, 512, 647, 697, 848
HpaII CCGG 3 cut(s) 449, 1564, 1646
HphI GGTGA 3 cut(s) 1061, 1603, 1609
Hpy166II GTNNAC 6 cut(s) 355, 951, 971, 1010, 1349, 1600
Hpy8I GTNNAC 6 cut(s) 355, 951, 971, 1010, 1349, 1600
Hpy99I CGWCG 1 cut(s) 1434
HpyAV CCTTC 7 cut(s) 310, 653, 857, 901, 1702, 1835, 1936
HpyCH4III ACNGT 6 cut(s) 266, 407, 1591, 1604, 1664, 1740
HpyCH4IV ACGT 2 cut(s) 1327, 1429
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 889, 1027, 1103
HpyF3I CTNAG 8 cut(s) 285, 366, 522, 604, 645, 1356, 1435, 1536
HpySE526I ACGT 2 cut(s) 1327, 1429
Hsp92I GRCGYC 1 cut(s) 1429
Hsp92II CATG 8 cut(s) 26, 89, 256, 824, 1049, 1200, 1246, 2047
HspAI GCGC 2 cut(s) 1097, 1099
Kpn2I TCCGGA 1 cut(s) 448
Kzo9I GATC 4 cut(s) 124, 445, 680, 1320
LmnI GCTCC 1 cut(s) 1693
Lsp1109I GCAGC 7 cut(s) 268, 742, 892, 1042, 1144, 1927, 2004
LweI GCATC 8 cut(s) 122, 516, 612, 650, 814, 1042, 1603, 2004
MabI ACCWGGT 3 cut(s) 1179, 1342, 1390
MaeI CTAG 5 cut(s) 132, 224, 345, 2067, 2071
MaeII ACGT 2 cut(s) 1327, 1429
MaeIII GTNAC 3 cut(s) 884, 923, 1215
MalI GATC 4 cut(s) 126, 447, 682, 1322
MboI GATC 4 cut(s) 124, 445, 680, 1320
MfeI CAATTG 2 cut(s) 617, 1552
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 3 cut(s) 357, 953, 973
MlsI TGGCCA 1 cut(s) 2007
MluCI AATT 8 cut(s) 14, 617, 1509, 1524, 1552, 1638, 1821, 1894
MluNI TGGCCA 1 cut(s) 2007
MlyI GAGTC 4 cut(s) 491, 656, 691, 857
MmeI TCCRAC 1 cut(s) 1348
Mox20I TGGCCA 1 cut(s) 2007
MroI TCCGGA 1 cut(s) 448
MroXI GAANNNNTTC 1 cut(s) 293
MscI TGGCCA 1 cut(s) 2007
MseI TTAA 6 cut(s) 309, 426, 1169, 1568, 1623, 1824
MslI CAYNNNNRTG 1 cut(s) 1050
Msp20I TGGCCA 1 cut(s) 2007
MspA1I CMGCKG 2 cut(s) 1157, 1783
MspCI CTTAAG 1 cut(s) 1622
MspI CCGG 3 cut(s) 449, 1564, 1646
MspR9I CCNGG 4 cut(s) 1181, 1344, 1392, 1646
MunI CAATTG 2 cut(s) 617, 1552
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 3 cut(s) 1181, 1344, 1392
MvnI CGCG 2 cut(s) 1097, 1099
MwoI GCNNNNNNNGC 4 cut(s) 278, 889, 1027, 1103
NciI CCSGG 1 cut(s) 1646
NcoI CCATGG 2 cut(s) 22, 1045
NdeII GATC 4 cut(s) 124, 445, 680, 1320
NlaIII CATG 8 cut(s) 26, 89, 256, 824, 1049, 1200, 1246, 2047
NlaIV GGNNCC 3 cut(s) 321, 447, 1111
NmuCI GTSAC 2 cut(s) 923, 1215
NspI RCATGY 2 cut(s) 256, 1200
PaeI GCATGC 1 cut(s) 1200
PagI TCATGA 3 cut(s) 85, 820, 1242
PauI GCGCGC 1 cut(s) 1097
PceI AGGCCT 1 cut(s) 1864
PctI GAATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 4 cut(s) 89, 137, 383, 512
PflFI GACNNNGTC 1 cut(s) 1957
PinAI ACCGGT 1 cut(s) 1563
PkrI GCNGC 8 cut(s) 283, 757, 882, 1032, 1159, 1785, 1917, 2019
PleI GAGTC 4 cut(s) 490, 655, 691, 856
PpsI GAGTC 4 cut(s) 490, 655, 691, 856
PpuMI RGGWCCY 1 cut(s) 319
PshBI ATTAAT 1 cut(s) 1824
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 3 cut(s) 1179, 1342, 1390
PspFI CCCAGC 1 cut(s) 1904
PspGI CCWGG 3 cut(s) 1179, 1342, 1390
PspN4I GGNNCC 3 cut(s) 321, 447, 1111
PspPI GGNCC 3 cut(s) 26, 319, 1349
PspPPI RGGWCCY 1 cut(s) 319
PsrI GAACNNNNNNTAC 2 cut(s) 136, 168
PstI CTGCAG 1 cut(s) 2022
PstNI CAGNNNCTG 4 cut(s) 656, 1160, 1786, 1936
PsuI RGATCY 1 cut(s) 445
PsyI GACNNNGTC 1 cut(s) 1957
PteI GCGCGC 1 cut(s) 1097
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 2 cut(s) 198, 206
RsaNI GTAC 2 cut(s) 197, 205
RseI CAYNNNNRTG 1 cut(s) 1050
SaqAI TTAA 6 cut(s) 309, 426, 1169, 1568, 1623, 1824
SatI GCNGC 8 cut(s) 282, 756, 881, 1031, 1158, 1784, 1916, 2018
Sau3AI GATC 4 cut(s) 124, 445, 680, 1320
Sau96I GGNCC 3 cut(s) 26, 319, 1349
SchI GAGTC 4 cut(s) 491, 656, 691, 857
ScrFI CCNGG 4 cut(s) 1181, 1344, 1392, 1646
SduI GDGCHC 3 cut(s) 357, 953, 973
SexAI ACCWGGT 3 cut(s) 1179, 1342, 1390
SfaNI GCATC 8 cut(s) 122, 516, 612, 650, 814, 1042, 1603, 2004
SfcI CTRYAG 3 cut(s) 759, 1404, 2018
SinI GGWCC 2 cut(s) 319, 1349
SmiMI CAYNNNNRTG 1 cut(s) 1050
SmlI CTYRAG 5 cut(s) 200, 627, 1016, 1622, 1751
SmoI CTYRAG 5 cut(s) 200, 627, 1016, 1622, 1751
SphI GCATGC 1 cut(s) 1200
Sse9I AATT 8 cut(s) 14, 617, 1509, 1524, 1552, 1638, 1821, 1894
SseBI AGGCCT 1 cut(s) 1864
SsiI CCGC 1 cut(s) 1783
SspMI CTAG 5 cut(s) 132, 224, 345, 2067, 2071
StuI AGGCCT 1 cut(s) 1864
StyD4I CCNGG 4 cut(s) 1179, 1342, 1390, 1644
StyI CCWWGG 2 cut(s) 22, 1045
TaaI ACNGT 6 cut(s) 266, 407, 1591, 1604, 1664, 1740
TaiI ACGT 2 cut(s) 1330, 1432
TaqI TCGA 2 cut(s) 671, 1399
TaqII GACCGA 1 cut(s) 1545
TasI AATT 8 cut(s) 14, 617, 1509, 1524, 1552, 1638, 1821, 1894
TatI WGTACW 2 cut(s) 196, 204
TauI GCSGC 1 cut(s) 1786
TfiI GAWTC 4 cut(s) 89, 137, 383, 512
Tru1I TTAA 6 cut(s) 309, 426, 1169, 1568, 1623, 1824
Tru9I TTAA 6 cut(s) 309, 426, 1169, 1568, 1623, 1824
TscAI CASTG 2 cut(s) 1743, 1886
TseFI GTSAC 2 cut(s) 923, 1215
TseI GCWGC 7 cut(s) 281, 755, 880, 1030, 1157, 1915, 2017
Tsp45I GTSAC 2 cut(s) 923, 1215
TspDTI ATGAA 6 cut(s) 102, 504, 1259, 1645, 1791, 2013
TspRI CASTG 2 cut(s) 1743, 1886
Tth111I GACNNNGTC 1 cut(s) 1957
Vha464I CTTAAG 1 cut(s) 1622
VneI GTGCAC 3 cut(s) 353, 949, 969
VpaK11BI GGWCC 2 cut(s) 319, 1349
VspI ATTAAT 1 cut(s) 1824
XapI RAATTY 2 cut(s) 14, 1894
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 2 cut(s) 256, 1200
XcmI CCANNNNNNNNNTGG 1 cut(s) 1343
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 5 cut(s) 132, 224, 345, 2067, 2071
ZraI GACGTC 1 cut(s) 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.