Rroxscaffold_1G00015630

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000175
Physical Location & Seq
Reverse (-)
19410535 .. 19412601
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00015630.1

Sequence Viewer

Length: 2067 bp
ATGGAAACATTCGTATTTGATCAGGGCTCAATTTCAAACTATTCTTTTCCAGATCATGGTAATGGGTTTGAAGTAAATCATGAACTCATCAACCCACTTTTCCTTCCTACGAACTTGGACCTTCTTAGTGATTCTAGTACATCTTTGAAGCCGACTTCAGACAGAAATGGTGGTGACAGTAGTGACTACAATAATCCTGTTCTCAAGTATATAAGTGATATTCTTCTCGAAGAGGACATTGAGGACAAGCCTTGTATGTTACAGGACTGTTTGGCCCTCCAAGCTGCTGAGAAATCTTTCTATGATGTCCTTAATCCGAAGGATGCTACAGTACTCAACCAAAGCTTTGAGAACTCGGATGATTACGGGGAGAGTTGGGATAGCAATGGTGGCTCTATTTTTGGTAAGACATATGGGGTTGCTGATCCTTCAGAATCTTGTCATTTCCAGTCTTCTCTTGTTGAGTGTCTATCAGATACCTCCTTGGTTTCTGATTCAAGTTCTAAGCTGCAAAGTCTTGGGAATTTTGGAAGGGTGGAGGAAGCAAACAAGTTCCATGAAAATTTTGAAATCATTGATGCGGAGAGGTACCAATTAATGCTTCAAGGTCCTCAGACACTAATACGATCAAATTTGGTGTCTGAGCAGCAGAGTGATCGGTACAACTCAGCAAATAGATCAGAGGCAAAAAAAAATCGTCAAAGGCAGGATGATGCTCATTGTCTGGAAGAATGGAGAAGCAACAAGCAGTCAGTTGCATATTTTACTGACGACTCTGAGCCGCAAGAAATGTTTGATAAGGCATTACTCTTTCAAGGTGTGAATCACGAAGCCGAGTGTAGTTCTCATCATGATTCTTTTAATGAGGGAAGTGGGCAGTGCAAAGGATCTAAGCCAGCACGTTCAAAGAGACAAAATAAAAAGGAGAAAGTAGTAGATTTGTGCACAATGTTAACTCAATGCGCGCAAGCTGTTGCAAGTTATGACCAACGAAATGCTACTGAATTACTGAAGCAGATTAGACAGCACTCTTCTCCCTATGGTGATGCAACCCAGAGATTAGCTCATTACTTTGCTGATGGCCTCGAGGTACGCTTAGCTGGCACTAGAACCCCGTCGTATTCGCCTATTGCAAGCATGCAGATATCAACTGTTGAAATATTGAAAAGTTACCAAACGTATGTCACTTCAACCCCTTTCAAGACCATGCCACACTTCTTTGCCAATATGACAATTATGAAACTAGCAGAAAAAGCAACAAGTCTTCACATTATTGATTTTGGTACTTCTTATGGTTTCCAATGGCCCTCTCTCATCCAATGTCTTTCAGAGAGACATGGTGGACCTCCTAAGCTTCGTATCACTACAATTGAGCTTCCCCAACCAGGATTTCGACCCACAGAGAGGGTTGAAGAAACTGGTCGTCGCTTAGCAAAGTATTCTGCAAGATTTAGTGTCCCATTTGAGTTCCATGTCATTGCTCAGAAATGGGAAACTGTTGGATTTGATGATCTTAAGATCGACAGAAATGAGCTGCTTGTGGTCAACTGTCTACACCGGTTAAAGCACGTTCCTGATGTGACAATGATGGGGAACAGTCCGAGAGACGCTGTCCTAAAGTTGATCAAGAAAATCAATCCAGAAATTTTCATTCATGGGGTTGTCAATGGGGCATACAACTCACCCTTCTTTCTCACACGTTTCAAACAGGCACTCTTCCACTACGATGCACTCTTTAATGTCTTTGAGGCCACCATTCCTTGTGAGGATCAACAACGACTGTTTTTCGAAAGAGCAGGATATGGTAGAGACATAATGAATGTAGTGGCGTGTGAGGGACTGGAAATAGTTGAAAGGCCTGAGACATATAAGAAGTGGCAGGTCAGGAATGTCAGGGCTGGGTTCAAGCAGCTCCCATTAGACCAGGAGCTGTTGAAGAAGGTTAAGACTATGTCGAAGGCGATGGGTTATCACAATGATTTTAGTATTGATGAAGACGAGCAGTGGATGCTGCAGGGATGGAAAGGAAGAGTCCTCATGGCTCTTTCCTTCTGGAAACGCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

688

Amino Acids

78.37

Weight (kDa)

5.6

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 316 - 686 5.9e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1085
Acc36I ACCTGC 1 cut(s) 1870
Acc65I GGTACC 1 cut(s) 588
AccB1I GGYRCC 1 cut(s) 588
AccB7I CCANNNNNTGG 1 cut(s) 56
AccI GTMKAC 1 cut(s) 1552
AccII CGCG 2 cut(s) 965, 2061
AciI CCGC 2 cut(s) 581, 782
AclWI GGATC 3 cut(s) 419, 895, 1776
AcsI RAATTY 4 cut(s) 523, 562, 631, 1644
AcuI CTGAAG 3 cut(s) 141, 414, 1031
AfaI GTAC 6 cut(s) 139, 333, 590, 662, 1092, 1285
AfiI CCNNNNNNNGG 3 cut(s) 56, 1385, 1404
AflII CTTAAG 1 cut(s) 1514
AflIII ACRYGT 1 cut(s) 1697
AgeI ACCGGT 1 cut(s) 1557
AjnI CCWGG 2 cut(s) 1384, 1923
AjuI GAANNNNNNNTTGG 4 cut(s) 585, 617, 1374, 1406
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 5 cut(s) 904, 1327, 1599, 1803, 1856
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 3 cut(s) 419, 895, 1776
AlwNI CAGNNNCTG 1 cut(s) 1930
Ama87I CYCGRG 1 cut(s) 1085
AoxI GGCC 5 cut(s) 273, 1081, 1304, 1749, 1856
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 6 cut(s) 284, 508, 646, 1534, 1909, 2011
ApoI RAATTY 4 cut(s) 523, 562, 631, 1644
ArsI GACNNNNNNTTYG 2 cut(s) 623, 655
AseI ATTAAT 1 cut(s) 596
AsiGI ACCGGT 1 cut(s) 1557
Asp700I GAANNNNTTC 1 cut(s) 296
Asp718I GGTACC 1 cut(s) 588
AspLEI GCGC 2 cut(s) 965, 967
AspS9I GGNCC 5 cut(s) 118, 274, 608, 1305, 1343
AsuHPI GGTGA 3 cut(s) 185, 1055, 1674
AsuII TTCGAA 1 cut(s) 1788
AvaI CYCGRG 1 cut(s) 1085
AvaII GGWCC 3 cut(s) 118, 608, 1343
BaeGI GKGCMC 1 cut(s) 947
BanI GGYRCC 1 cut(s) 588
BanII GRGCYC 1 cut(s) 29
BbsI GAAGAC 3 cut(s) 444, 1256, 2001
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 6 cut(s) 271, 495, 658, 1521, 1921, 1998
BccI CCATC 4 cut(s) 1073, 1582, 1957, 2013
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BciT130I CCWGG 2 cut(s) 1386, 1925
BclI TGATCA 2 cut(s) 19, 1623
BcoDI GTCTC 5 cut(s) 904, 1327, 1599, 1803, 1856
BfaI CTAG 3 cut(s) 135, 1107, 1244
BfmI CTRYAG 2 cut(s) 327, 2012
BfrI CTTAAG 1 cut(s) 1514
BfuAI ACCTGC 1 cut(s) 1870
BisI GCNGC 7 cut(s) 285, 509, 647, 782, 1535, 1910, 2012
BlpI GCTNAGC 2 cut(s) 1096, 1429
BlsI GCNGC 7 cut(s) 286, 510, 648, 783, 1536, 1911, 2013
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 2 cut(s) 1386, 1925
Bme18I GGWCC 3 cut(s) 118, 608, 1343
BmeT110I CYCGRG 1 cut(s) 1085
BmgT120I GGNCC 5 cut(s) 118, 274, 608, 1305, 1343
BmiI GGNNCC 1 cut(s) 590
BmrFI CCNGG 2 cut(s) 1386, 1925
BmsI GCATC 6 cut(s) 313, 568, 703, 1036, 1717, 1998
BpiI GAAGAC 3 cut(s) 444, 1256, 2001
BplI GAGNNNNNCTC 2 cut(s) 1048, 1080
Bpu10I CCTNAGC 1 cut(s) 1350
Bpu1102I GCTNAGC 2 cut(s) 1096, 1429
Bpu14I TTCGAA 1 cut(s) 1788
BpuEI CTTGAG 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 483
BsaWI WCCGGW 1 cut(s) 1557
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 1385, 1404
Bse118I RCCGGY 1 cut(s) 1557
Bse1I ACTGG 3 cut(s) 448, 1423, 1845
Bse3DI GCAATG 2 cut(s) 391, 1476
BseBI CCWGG 2 cut(s) 1386, 1925
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 6 cut(s) 328, 364, 715, 1314, 2013, 2024
BseLI CCNNNNNNNGG 3 cut(s) 56, 1385, 1404
BseMI GCAATG 2 cut(s) 391, 1476
BseMII CTCAG 7 cut(s) 279, 626, 633, 681, 768, 1496, 1851
BseNI ACTGG 3 cut(s) 448, 1423, 1845
BsePI GCGCGC 1 cut(s) 963
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 6 cut(s) 271, 495, 658, 1521, 1921, 1998
BseYI CCCAGC 1 cut(s) 1898
Bsh1236I CGCG 2 cut(s) 965, 2061
BshFI GGCC 5 cut(s) 275, 1083, 1306, 1751, 1858
BshNI GGYRCC 1 cut(s) 588
BshTI ACCGGT 1 cut(s) 1557
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 1085
BsiSI CCGG 1 cut(s) 1558
BslFI GGGAC 2 cut(s) 1442, 1851
BslI CCNNNNNNNGG 3 cut(s) 56, 1385, 1404
BsmAI GTCTC 5 cut(s) 904, 1327, 1599, 1803, 1856
BsmBI CGTCTC 1 cut(s) 1599
BsmFI GGGAC 2 cut(s) 1442, 1851
BsnI GGCC 5 cut(s) 275, 1083, 1306, 1751, 1858
BsoBI CYCGRG 1 cut(s) 1085
Bsp119I TTCGAA 1 cut(s) 1788
Bsp1286I GDGCHC 2 cut(s) 29, 947
Bsp1720I GCTNAGC 2 cut(s) 1096, 1429
BspACI CCGC 2 cut(s) 581, 782
BspANI GGCC 5 cut(s) 275, 1083, 1306, 1751, 1858
BspCNI CTCAG 7 cut(s) 280, 625, 634, 680, 769, 1495, 1852
BspFNI CGCG 2 cut(s) 965, 2061
BspHI TCATGA 2 cut(s) 79, 850
BspLI GGNNCC 1 cut(s) 590
BspMAI CTGCAG 1 cut(s) 2016
BspMI ACCTGC 1 cut(s) 1870
BspPI GGATC 3 cut(s) 419, 895, 1776
BspT104I TTCGAA 1 cut(s) 1788
BspT107I GGYRCC 1 cut(s) 588
BspTI CTTAAG 1 cut(s) 1514
BsrDI GCAATG 2 cut(s) 391, 1476
BsrFI RCCGGY 1 cut(s) 1557
BsrI ACTGG 3 cut(s) 448, 1423, 1845
BssAI RCCGGY 1 cut(s) 1557
BssECI CCNNGG 1 cut(s) 483
BssHII GCGCGC 1 cut(s) 963
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 2 cut(s) 1386, 1925
Bst4CI ACNGT 8 cut(s) 179, 269, 331, 1153, 1498, 1550, 1598, 1782
Bst6I CTCTTC 4 cut(s) 225, 1036, 1721, 2023
BstAFI CTTAAG 1 cut(s) 1514
BstAPI GCANNNNNTGC 1 cut(s) 2008
BstBI TTCGAA 1 cut(s) 1788
BstC8I GCNNGC 6 cut(s) 897, 965, 969, 1102, 1135, 1139
BstF5I GGATG 6 cut(s) 328, 364, 715, 1314, 2013, 2024
BstFNI CGCG 2 cut(s) 965, 2061
BstHHI GCGC 2 cut(s) 965, 967
BstMAI GTCTC 5 cut(s) 904, 1327, 1599, 1803, 1856
BstMWI GCNNNNNNNGC 5 cut(s) 281, 390, 1101, 1253, 2008
BstNI CCWGG 2 cut(s) 1386, 1925
BstNSI RCATGY 1 cut(s) 1141
BstSCI CCNGG 2 cut(s) 1384, 1923
BstSFI CTRYAG 2 cut(s) 327, 2012
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 2 cut(s) 965, 2061
BstV1I GCAGC 6 cut(s) 271, 495, 658, 1521, 1921, 1998
BstV2I GAAGAC 3 cut(s) 444, 1256, 2001
BstX2I RGATCY 1 cut(s) 887
BstYI RGATCY 1 cut(s) 887
BsuRI GGCC 5 cut(s) 275, 1083, 1306, 1751, 1858
BtgZI GCGATG 1 cut(s) 1976
BtsCI GGATG 6 cut(s) 328, 364, 715, 1314, 2013, 2024
BtsI GCAGTG 2 cut(s) 884, 2009
BtsIMutI CAGTG 2 cut(s) 884, 2009
BveI ACCTGC 1 cut(s) 1870
Cac8I GCNNGC 6 cut(s) 897, 965, 969, 1102, 1135, 1139
CaiI CAGNNNCTG 1 cut(s) 1930
CciI TCATGA 2 cut(s) 79, 850
CfoI GCGC 2 cut(s) 965, 967
Cfr10I RCCGGY 1 cut(s) 1557
Cfr13I GGNCC 5 cut(s) 118, 274, 608, 1305, 1343
CseI GACGC 1 cut(s) 1616
Csp6I GTAC 6 cut(s) 138, 332, 589, 661, 1091, 1284
CspAI ACCGGT 1 cut(s) 1557
CviQI GTAC 6 cut(s) 138, 332, 589, 661, 1091, 1284
Eam1104I CTCTTC 4 cut(s) 225, 1036, 1721, 2023
EarI CTCTTC 4 cut(s) 225, 1036, 1721, 2023
Eco130I CCWWGG 1 cut(s) 483
Eco147I AGGCCT 1 cut(s) 1858
Eco24I GRGCYC 1 cut(s) 29
Eco32I GATATC 1 cut(s) 1146
Eco47I GGWCC 3 cut(s) 118, 608, 1343
Eco57I CTGAAG 3 cut(s) 141, 414, 1031
Eco88I CYCGRG 1 cut(s) 1085
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 1384, 1923
EcoRV GATATC 1 cut(s) 1146
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 1599
FaqI GGGAC 2 cut(s) 1442, 1851
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 2 cut(s) 19, 1623
FblI GTMKAC 1 cut(s) 1552
Fnu4HI GCNGC 7 cut(s) 285, 509, 647, 782, 1535, 1910, 2012
FokI GGATG 6 cut(s) 335, 371, 722, 1301, 2020, 2031
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 7 cut(s) 285, 509, 647, 782, 1535, 1910, 2012
FspBI CTAG 3 cut(s) 135, 1107, 1244
GlaI GCGC 2 cut(s) 964, 966
GluI GCNGC 7 cut(s) 285, 509, 647, 782, 1535, 1910, 2012
GsaI CCCAGC 1 cut(s) 1902
HaeIII GGCC 5 cut(s) 275, 1083, 1306, 1751, 1858
HapII CCGG 1 cut(s) 1558
HgaI GACGC 1 cut(s) 1616
HhaI GCGC 2 cut(s) 965, 967
Hin6I GCGC 2 cut(s) 963, 965
HinP1I GCGC 2 cut(s) 963, 965
HincII GTYRAC 2 cut(s) 954, 1546
HindII GTYRAC 2 cut(s) 954, 1546
HindIII AAGCTT 2 cut(s) 343, 1352
HinfI GANTC 7 cut(s) 131, 434, 494, 773, 823, 854, 2031
HpaI GTTAAC 1 cut(s) 954
HpaII CCGG 1 cut(s) 1558
HphI GGTGA 3 cut(s) 185, 1055, 1674
Hpy166II GTNNAC 5 cut(s) 945, 954, 1343, 1546, 1553
Hpy8I GTNNAC 5 cut(s) 945, 954, 1343, 1546, 1553
Hpy99I CGWCG 2 cut(s) 1120, 1428
HpyAV CCTTC 9 cut(s) 113, 131, 313, 438, 525, 1696, 1933, 1951, 2059
HpyCH4III ACNGT 8 cut(s) 179, 269, 331, 1153, 1498, 1550, 1598, 1782
HpyCH4IV ACGT 4 cut(s) 901, 1178, 1569, 1699
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 390, 1101, 1253, 2008
HpySE526I ACGT 4 cut(s) 901, 1178, 1569, 1699
HspAI GCGC 2 cut(s) 963, 965
KpnI GGTACC 1 cut(s) 592
Ksp22I TGATCA 2 cut(s) 19, 1623
KspAI GTTAAC 1 cut(s) 954
LmnI GCTCC 2 cut(s) 1917, 1927
Lsp1109I GCAGC 6 cut(s) 271, 495, 658, 1521, 1921, 1998
LweI GCATC 6 cut(s) 313, 568, 703, 1036, 1717, 1998
MaeI CTAG 3 cut(s) 135, 1107, 1244
MaeII ACGT 4 cut(s) 901, 1178, 1569, 1699
MaeIII GTNAC 6 cut(s) 173, 182, 258, 1169, 1183, 1579
MfeI CAATTG 1 cut(s) 1368
MflI RGATCY 1 cut(s) 887
MhlI GDGCHC 2 cut(s) 29, 947
MluCI AATT 9 cut(s) 30, 523, 562, 593, 631, 1004, 1233, 1368, 1644
MlyI GAGTC 2 cut(s) 767, 2040
MmeI TCCRAC 1 cut(s) 1480
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 8 cut(s) 312, 596, 861, 953, 1515, 1562, 1737, 1944
MslI CAYNNNNRTG 2 cut(s) 60, 1725
MspCI CTTAAG 1 cut(s) 1514
MspI CCGG 1 cut(s) 1558
MspR9I CCNGG 2 cut(s) 1386, 1925
MunI CAATTG 1 cut(s) 1368
MvaI CCWGG 2 cut(s) 1386, 1925
MvnI CGCG 2 cut(s) 965, 2061
MwoI GCNNNNNNNGC 5 cut(s) 281, 390, 1101, 1253, 2008
NdeI CATATG 1 cut(s) 412
NlaIV GGNNCC 1 cut(s) 590
NmeAIII GCCGAG 1 cut(s) 859
NmuCI GTSAC 4 cut(s) 173, 182, 1183, 1579
NspI RCATGY 1 cut(s) 1141
NspV TTCGAA 1 cut(s) 1788
PaeI GCATGC 1 cut(s) 1141
PaeR7I CTCGAG 1 cut(s) 1085
PagI TCATGA 2 cut(s) 79, 850
PauI GCGCGC 1 cut(s) 963
PceI AGGCCT 1 cut(s) 1858
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 5 cut(s) 131, 434, 494, 823, 854
PflFI GACNNNGTC 2 cut(s) 1610, 1951
PflMI CCANNNNNTGG 1 cut(s) 56
PinAI ACCGGT 1 cut(s) 1557
PkrI GCNGC 7 cut(s) 286, 510, 648, 783, 1536, 1911, 2013
PleI GAGTC 2 cut(s) 767, 2039
PpsI GAGTC 2 cut(s) 767, 2039
PpuMI RGGWCCY 1 cut(s) 608
PshBI ATTAAT 1 cut(s) 596
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 1384, 1923
PspFI CCCAGC 1 cut(s) 1898
PspGI CCWGG 2 cut(s) 1384, 1923
PspN4I GGNNCC 1 cut(s) 590
PspPI GGNCC 5 cut(s) 118, 274, 608, 1305, 1343
PspPPI RGGWCCY 1 cut(s) 608
PspXI VCTCGAGB 1 cut(s) 1085
PstI CTGCAG 1 cut(s) 2016
PstNI CAGNNNCTG 1 cut(s) 1930
PsuI RGATCY 1 cut(s) 887
PsyI GACNNNGTC 2 cut(s) 1610, 1951
PteI GCGCGC 1 cut(s) 963
RsaI GTAC 6 cut(s) 139, 333, 590, 662, 1092, 1285
RsaNI GTAC 6 cut(s) 138, 332, 589, 661, 1091, 1284
RseI CAYNNNNRTG 2 cut(s) 60, 1725
SaqAI TTAA 8 cut(s) 312, 596, 861, 953, 1515, 1562, 1737, 1944
SatI GCNGC 7 cut(s) 285, 509, 647, 782, 1535, 1910, 2012
Sau96I GGNCC 5 cut(s) 118, 274, 608, 1305, 1343
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 2 cut(s) 767, 2040
ScrFI CCNGG 2 cut(s) 1386, 1925
SduI GDGCHC 2 cut(s) 29, 947
SfaNI GCATC 6 cut(s) 313, 568, 703, 1036, 1717, 1998
SfcI CTRYAG 2 cut(s) 327, 2012
Sfr274I CTCGAG 1 cut(s) 1085
SfuI TTCGAA 1 cut(s) 1788
SinI GGWCC 3 cut(s) 118, 608, 1343
SlaI CTCGAG 1 cut(s) 1085
SmiMI CAYNNNNRTG 2 cut(s) 60, 1725
SmlI CTYRAG 3 cut(s) 203, 1085, 1514
SmoI CTYRAG 3 cut(s) 203, 1085, 1514
SphI GCATGC 1 cut(s) 1141
Sse9I AATT 9 cut(s) 30, 523, 562, 593, 631, 1004, 1233, 1368, 1644
SseBI AGGCCT 1 cut(s) 1858
SsiI CCGC 2 cut(s) 581, 782
SspI AATATT 1 cut(s) 1161
SspMI CTAG 3 cut(s) 135, 1107, 1244
StuI AGGCCT 1 cut(s) 1858
StyD4I CCNGG 2 cut(s) 1384, 1923
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 8 cut(s) 179, 269, 331, 1153, 1498, 1550, 1598, 1782
TaiI ACGT 4 cut(s) 904, 1181, 1572, 1702
TaqI TCGA 6 cut(s) 228, 1086, 1393, 1521, 1788, 1955
TasI AATT 9 cut(s) 30, 523, 562, 593, 631, 1004, 1233, 1368, 1644
TatI WGTACW 2 cut(s) 137, 331
TauI GCSGC 1 cut(s) 784
TfiI GAWTC 5 cut(s) 131, 434, 494, 823, 854
Tru1I TTAA 8 cut(s) 312, 596, 861, 953, 1515, 1562, 1737, 1944
Tru9I TTAA 8 cut(s) 312, 596, 861, 953, 1515, 1562, 1737, 1944
TscAI CASTG 2 cut(s) 884, 2009
TseFI GTSAC 4 cut(s) 173, 182, 1183, 1579
TseI GCWGC 6 cut(s) 284, 508, 646, 1534, 1909, 2011
Tsp45I GTSAC 4 cut(s) 173, 182, 1183, 1579
TspDTI ATGAA 7 cut(s) 96, 573, 1253, 1639, 1643, 1832, 2007
TspRI CASTG 2 cut(s) 884, 2009
Tth111I GACNNNGTC 2 cut(s) 1610, 1951
Van91I CCANNNNNTGG 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 1514
VneI GTGCAC 1 cut(s) 943
VpaK11BI GGWCC 3 cut(s) 118, 608, 1343
VspI ATTAAT 1 cut(s) 596
XapI RAATTY 4 cut(s) 523, 562, 631, 1644
XceI RCATGY 1 cut(s) 1141
XhoI CTCGAG 1 cut(s) 1085
XmiI GTMKAC 1 cut(s) 1552
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 3 cut(s) 135, 1107, 1244
ZrmI AGTACT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.