Rw5G040510

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000175
Physical Location & Seq
Forward (+)
71519283 .. 71521468
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040510.1

Sequence Viewer

Length: 2022 bp
ATGGATGAATTTCCAAGTTCCATGAATAATTTCATGTTTTACCAGAACTCAATGCCAACTTCAGGTCAGAATATCCTTGTTACTGAATCTGATCAACACAATCATGACTTCAGTGATCCCAGTTTCTTTCCATCCAATCCAAACCCTCCTTCTCGTGATGATTCATCTTCATCTTTGGGTTTGAGCTCAGAGGGGGAGTATTACTCTCCCTCTATTGCTACTCTCCAATACATAAGTCAGATGCTTATGGAAGAAGACTTGCAGAACAAACCCTGCATGCTTCAGGACTGTATGGCCCTCCAAGCCACTGAGAAATCCTTACATGATGTCCTAGTTCAGCAATATCCTTCTTCGACTAATCACCTTCTCACTTCTTCCACTCAGCAAAATGTTGAGATATCTGATGATTTCATTAACCACAGCAGTAATAGTTCCACTGCCGCTAGGAACTGGGATGATGGCTCAGGTTGGATTTCTTTACAAAATGTTCTAGAATCCTTTCCTGTGGAAAATACTACCCTTTCAGTTCCAAATCCTTTTTCCTCAAATGGCAAGAGTGAAATCATAGATCAAGAAAGGGACTCATCATTTCTCATACAGCCAAGGGAGCTGACTGAGGATGGGAATGACTCAACAAACAGATCAAGGAGCAAGAAAGATCGTCAGTGGGAGGATGGTGATTATCAAGAACAAGGGAGGAGCAACAAGCAGACCGCACTTTATGCAGCTGAATCTGAGCCACCAGAGATGTTGGATCAGGTACTGCTCTATCAATATCAAAACTCAGGTTCTTCTCTTCAGGAATCTGATCAGCTTAAAACGAGAAGTGGATTTAGAGGATTAAAAGGCAAAAGAACAGGAAAGAAAAAAATGGATGACAACGGAGCAGTAGTGGATTTTCAGACACTTCTAACTCAATGTGCAAAAGCTGTTGCAAGCTATGACACAAGGACAGCGAATGAACAACTCAAGCTGATAAGGCAGCACTCATCTCCCCATGGTGATGGAACCCAACGATTAGCTCATTACTTTGCCAATGGCCTCGAAGAACGCTTGATCGCTGCGGTTCCCTTGTACAATCCCGTTTCCCTTTTCAGTTATAAGATGTCAGCTGCTGATATCTTGAAAGCTTACCAGACTTATATCAAAGCATGCCCTTTCAAGCTGATGTCAAATATCTACGCTAACAAAACTATTTTCAAGCTAGCAGAGAAAGTAACGAGGCTGCACATAATCGATTTTGGTATTCTCTATGGCTACAAATGGCCTTCCCTTATCCAGATGCTTGCGAAAAGACCCTCCGGACCTCCAATGCTTCACATTACTGGTATTGAATTTCCCCAATCAGGATTTCGACCGTCTGGGAGGCTTGAAGAGACGGGGAACCGCCTAGCGAAATACTGCAAGAGATTCAATGTCCCATTTGAGTACAATTTCATAGCACAGAGTTGGGACACAATTCAATATGAAGATATCAAACTTGACAGAGATGAGTTGACTGTAGTGAACTGCTTGTACAGGCTAAAGAACTTACATGATGAAACTGTAATCAACAGTCCAAGGGATACAGTTTTGAAGCTTATCAGGAGAATCAACCCTGATATCTTCATCCATGGAGTGGTTAATGGAGCCTACAATGCACCCTTCTTCAACATACGGTTCCGGGAGGCACTCTACTATTTCTCTTCTTTGTTTGATGTGTTTGAGGAGACTTTGCCCAGAGAAGATCAACAGAGGCTGCTCTACGAACAAGAGATATTTGGTAGAGATATTATCAATGTGATAGCATGTGAGGGTTCCAGGAGGCTTGAAAGGCCTGAAACATACAAGCAGTGGCAGATAAGAAACACTAGAGCTGGGTTCAAGCAGTTACCATTGAACCAGGAGATCGTGAAGAAAGTTAAGAATATTGTAAGGTTAGATTACCATGAGGACTTTGTTGTGGACAAAGATGGCAAGTGGCTCCTGCAGGGATGGAAAGGCCGAATAATACATGCTATTTGTTGCTGGAAATCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

673

Amino Acids

77.21

Weight (kDa)

5.87

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 301 - 671 1e-115 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1101
AccB7I CCANNNNNTGG 1 cut(s) 1618
AccIII TCCGGA 1 cut(s) 1301
AciI CCGC 4 cut(s) 441, 714, 1064, 1387
AclWI GGATC 2 cut(s) 110, 762
AcsI RAATTY 2 cut(s) 8, 1334
AcuI CTGAAG 4 cut(s) 45, 94, 266, 782
AfaI GTAC 4 cut(s) 762, 1076, 1430, 1517
AfiI CCNNNNNNNGG 3 cut(s) 62, 1346, 1618
AjnI CCWGG 2 cut(s) 1799, 1881
AjuI GAANNNNNNNTTGG 6 cut(s) 133, 165, 523, 555, 1335, 1367
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 2 cut(s) 1370, 1703
AlwI GGATC 2 cut(s) 110, 762
AlwNI CAGNNNCTG 3 cut(s) 763, 1115, 1738
Aor13HI TCCGGA 1 cut(s) 1301
AoxI GGCC 5 cut(s) 294, 1039, 1265, 1814, 1981
ApeKI GCWGC 6 cut(s) 725, 982, 1061, 1112, 1225, 1738
ApoI RAATTY 2 cut(s) 8, 1334
Asp700I GAANNNNTTC 2 cut(s) 29, 498
AspS9I GGNCC 2 cut(s) 295, 1304
AsuC2I CCSGG 1 cut(s) 1664
AsuHPI GGTGA 3 cut(s) 353, 689, 1013
AsuNHI GCTAGC 1 cut(s) 1204
AvaII GGWCC 1 cut(s) 1304
BanII GRGCYC 1 cut(s) 188
BauI CACGAG 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 6 cut(s) 737, 994, 1048, 1099, 1212, 1725
BccI CCATC 7 cut(s) 139, 452, 614, 668, 998, 1946, 1968
BciT130I CCWGG 2 cut(s) 1801, 1883
BciVI GTATCC 1 cut(s) 1558
BclI TGATCA 2 cut(s) 91, 808
BcnI CCSGG 1 cut(s) 1664
BcoDI GTCTC 2 cut(s) 1370, 1703
BfaI CTAG 6 cut(s) 332, 444, 491, 1205, 1391, 1851
BfmI CTRYAG 2 cut(s) 1500, 1967
BfuI GTATCC 1 cut(s) 1558
BisI GCNGC 7 cut(s) 441, 726, 983, 1062, 1113, 1226, 1739
BlsI GCNGC 7 cut(s) 442, 727, 984, 1063, 1114, 1227, 1740
Bme1390I CCNGG 3 cut(s) 1664, 1801, 1883
Bme18I GGWCC 1 cut(s) 1304
BmgT120I GGNCC 2 cut(s) 295, 1304
BmiI GGNNCC 7 cut(s) 1009, 1068, 1385, 1630, 1661, 1798, 1964
BmrFI CCNGG 3 cut(s) 1664, 1801, 1883
BmrI ACTGGG 2 cut(s) 114, 460
BmsI GCATC 2 cut(s) 231, 1272
BmtI GCTAGC 1 cut(s) 1208
BmuI ACTGGG 2 cut(s) 114, 460
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 188, 220
Bpu10I CCTNAGC 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 953
BpuMI CCSGG 1 cut(s) 1664
Bsa29I ATCGAT 1 cut(s) 1236
BsaJI CCNNGG 4 cut(s) 602, 997, 1559, 1612
BsaWI WCCGGW 1 cut(s) 1301
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 1346, 1618
Bse1I ACTGG 3 cut(s) 120, 455, 1330
BseAI TCCGGA 1 cut(s) 1301
BseBI CCWGG 2 cut(s) 1801, 1883
BseCI ATCGAT 1 cut(s) 1236
BseDI CCNNGG 4 cut(s) 602, 997, 1559, 1612
BseGI GGATG 8 cut(s) 10, 131, 460, 625, 679, 880, 1608, 1979
BseLI CCNNNNNNNGG 3 cut(s) 62, 1346, 1618
BseMII CTCAG 7 cut(s) 201, 300, 395, 477, 606, 726, 798
BseNI ACTGG 3 cut(s) 120, 455, 1330
BseRI GAGGAG 2 cut(s) 712, 1721
BseXI GCAGC 6 cut(s) 737, 994, 1048, 1099, 1212, 1725
BseYI CCCAGC 1 cut(s) 1856
BsgI GTGCAG 1 cut(s) 1211
Bsh1285I CGRYCG 1 cut(s) 1358
BshFI GGCC 5 cut(s) 296, 1041, 1267, 1816, 1983
BshVI ATCGAT 1 cut(s) 1236
BsiEI CGRYCG 1 cut(s) 1358
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 2 cut(s) 1302, 1663
BslFI GGGAC 3 cut(s) 593, 1403, 1466
BslI CCNNNNNNNGG 3 cut(s) 62, 1346, 1618
BsmAI GTCTC 2 cut(s) 1370, 1703
BsmBI CGTCTC 1 cut(s) 1370
BsmFI GGGAC 3 cut(s) 593, 1403, 1466
BsnI GGCC 5 cut(s) 296, 1041, 1267, 1816, 1983
Bsp1286I GDGCHC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 1301
Bsp1407I TGTACA 2 cut(s) 1074, 1515
Bsp19I CCATGG 2 cut(s) 997, 1612
BspACI CCGC 4 cut(s) 441, 714, 1064, 1387
BspANI GGCC 5 cut(s) 296, 1041, 1267, 1816, 1983
BspCNI CTCAG 7 cut(s) 200, 301, 394, 476, 607, 727, 797
BspDI ATCGAT 1 cut(s) 1236
BspEI TCCGGA 1 cut(s) 1301
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 7 cut(s) 1009, 1068, 1385, 1630, 1661, 1798, 1964
BspMAI CTGCAG 1 cut(s) 1971
BspOI GCTAGC 1 cut(s) 1208
BspPI GGATC 2 cut(s) 110, 762
BsrGI TGTACA 2 cut(s) 1074, 1515
BsrI ACTGG 3 cut(s) 120, 455, 1330
BssECI CCNNGG 4 cut(s) 602, 997, 1559, 1612
BssSI CACGAG 1 cut(s) 153
BssT1I CCWWGG 4 cut(s) 602, 997, 1559, 1612
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 1801, 1883
Bst4CI ACNGT 7 cut(s) 290, 1359, 1501, 1546, 1556, 1570, 1659
Bst6I CTCTTC 3 cut(s) 801, 1368, 1690
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 2 cut(s) 1074, 1515
BstC8I GCNNGC 5 cut(s) 278, 937, 1153, 1206, 1287
BstDEI CTNAG 7 cut(s) 187, 309, 381, 463, 615, 735, 784
BstDSI CCRYGG 2 cut(s) 997, 1612
BstF5I GGATG 8 cut(s) 10, 131, 460, 625, 679, 880, 1608, 1979
BstMAI GTCTC 2 cut(s) 1370, 1703
BstMCI CGRYCG 1 cut(s) 1358
BstMWI GCNNNNNNNGC 6 cut(s) 302, 607, 722, 979, 1637, 1813
BstNI CCWGG 2 cut(s) 1801, 1883
BstNSI RCATGY 4 cut(s) 280, 1155, 1791, 1997
BstSCI CCNGG 3 cut(s) 1662, 1799, 1881
BstSFI CTRYAG 2 cut(s) 1500, 1967
BstV1I GCAGC 6 cut(s) 737, 994, 1048, 1099, 1212, 1725
BstV2I GAAGAC 1 cut(s) 261
BstXI CCANNNNNNTGG 1 cut(s) 1004
Bsu15I ATCGAT 1 cut(s) 1236
BsuI GTATCC 1 cut(s) 1558
BsuRI GGCC 5 cut(s) 296, 1041, 1267, 1816, 1983
BsuTUI ATCGAT 1 cut(s) 1236
BtgI CCRYGG 2 cut(s) 997, 1612
BtsCI GGATG 8 cut(s) 10, 131, 460, 625, 679, 880, 1608, 1979
BtsI GCAGTG 2 cut(s) 435, 1838
BtsIMutI CAGTG 5 cut(s) 118, 306, 435, 671, 1838
Cac8I GCNNGC 5 cut(s) 278, 937, 1153, 1206, 1287
CaiI CAGNNNCTG 3 cut(s) 763, 1115, 1738
CciI TCATGA 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 295, 1304
ClaI ATCGAT 1 cut(s) 1236
Csp6I GTAC 4 cut(s) 761, 1075, 1429, 1516
CviQI GTAC 4 cut(s) 761, 1075, 1429, 1516
DdeI CTNAG 7 cut(s) 187, 309, 381, 463, 615, 735, 784
Eam1104I CTCTTC 3 cut(s) 801, 1368, 1690
EarI CTCTTC 3 cut(s) 801, 1368, 1690
Ecl136II GAGCTC 1 cut(s) 186
Eco130I CCWWGG 4 cut(s) 602, 997, 1559, 1612
Eco147I AGGCCT 1 cut(s) 1816
Eco24I GRGCYC 1 cut(s) 188
Eco32I GATATC 4 cut(s) 399, 1120, 1474, 1603
Eco47I GGWCC 1 cut(s) 1304
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 4 cut(s) 45, 94, 266, 782
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 1799, 1881
EcoRV GATATC 4 cut(s) 399, 1120, 1474, 1603
EcoT14I CCWWGG 4 cut(s) 602, 997, 1559, 1612
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 4 cut(s) 602, 997, 1559, 1612
Esp3I CGTCTC 1 cut(s) 1370
FaqI GGGAC 3 cut(s) 593, 1403, 1466
FbaI TGATCA 2 cut(s) 91, 808
Fnu4HI GCNGC 7 cut(s) 441, 726, 983, 1062, 1113, 1226, 1739
FokI GGATG 8 cut(s) 17, 118, 467, 632, 686, 887, 1595, 1986
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 7 cut(s) 441, 726, 983, 1062, 1113, 1226, 1739
FspBI CTAG 6 cut(s) 332, 444, 491, 1205, 1391, 1851
GluI GCNGC 7 cut(s) 441, 726, 983, 1062, 1113, 1226, 1739
GsaI CCCAGC 1 cut(s) 1860
HaeIII GGCC 5 cut(s) 296, 1041, 1267, 1816, 1983
HapII CCGG 2 cut(s) 1302, 1663
HincII GTYRAC 1 cut(s) 1497
HindII GTYRAC 1 cut(s) 1497
HindIII AAGCTT 2 cut(s) 1128, 1577
HinfI GANTC 9 cut(s) 86, 161, 494, 581, 629, 731, 803, 1410, 1590
HpaII CCGG 2 cut(s) 1302, 1663
HphI GGTGA 3 cut(s) 353, 689, 1013
Hpy166II GTNNAC 3 cut(s) 1497, 1507, 1945
Hpy188I TCNGA 8 cut(s) 69, 91, 190, 240, 403, 736, 808, 903
Hpy8I GTNNAC 3 cut(s) 1497, 1507, 1945
HpyAV CCTTC 5 cut(s) 159, 357, 374, 1278, 1654
HpyCH4III ACNGT 7 cut(s) 290, 1359, 1501, 1546, 1556, 1570, 1659
HpyCH4V TGCA 9 cut(s) 262, 276, 725, 923, 935, 1228, 1404, 1640, 1969
HpyF10VI GCNNNNNNNGC 6 cut(s) 302, 607, 722, 979, 1637, 1813
HpyF3I CTNAG 7 cut(s) 187, 309, 381, 463, 615, 735, 784
Kpn2I TCCGGA 1 cut(s) 1301
Ksp22I TGATCA 2 cut(s) 91, 808
LmnI GCTCC 6 cut(s) 607, 648, 699, 884, 1628, 1968
Lsp1109I GCAGC 6 cut(s) 737, 994, 1048, 1099, 1212, 1725
LweI GCATC 2 cut(s) 231, 1272
MaeI CTAG 6 cut(s) 332, 444, 491, 1205, 1391, 1851
MaeIII GTNAC 3 cut(s) 79, 1216, 1869
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 5 cut(s) 8, 28, 1334, 1432, 1458
MlyI GAGTC 2 cut(s) 575, 623
MmeI TCCRAC 2 cut(s) 449, 732
MroI TCCGGA 1 cut(s) 1301
MroXI GAANNNNTTC 2 cut(s) 29, 498
MseI TTAA 5 cut(s) 414, 816, 842, 1623, 1902
MslI CAYNNNNRTG 2 cut(s) 102, 1002
MspA1I CMGCKG 2 cut(s) 728, 1112
MspI CCGG 2 cut(s) 1302, 1663
MspR9I CCNGG 3 cut(s) 1664, 1801, 1883
MvaI CCWGG 2 cut(s) 1801, 1883
MwoI GCNNNNNNNGC 6 cut(s) 302, 607, 722, 979, 1637, 1813
NciI CCSGG 1 cut(s) 1664
NcoI CCATGG 2 cut(s) 997, 1612
NheI GCTAGC 1 cut(s) 1204
NlaIV GGNNCC 7 cut(s) 1009, 1068, 1385, 1630, 1661, 1798, 1964
NspI RCATGY 4 cut(s) 280, 1155, 1791, 1997
PaeI GCATGC 2 cut(s) 280, 1155
PagI TCATGA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 1816
PdmI GAANNNNTTC 2 cut(s) 29, 498
PfeI GAWTC 7 cut(s) 86, 161, 494, 731, 803, 1410, 1590
PflMI CCANNNNNTGG 1 cut(s) 1618
PfoI TCCNGGA 2 cut(s) 1662, 1799
PkrI GCNGC 7 cut(s) 442, 727, 984, 1063, 1114, 1227, 1740
PleI GAGTC 2 cut(s) 575, 623
PpsI GAGTC 2 cut(s) 575, 623
PsiI TTATAA 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 1799, 1881
PspFI CCCAGC 1 cut(s) 1856
PspGI CCWGG 2 cut(s) 1799, 1881
PspN4I GGNNCC 7 cut(s) 1009, 1068, 1385, 1630, 1661, 1798, 1964
PspPI GGNCC 2 cut(s) 295, 1304
PsrI GAACNNNNNNTAC 2 cut(s) 1499, 1531
PstI CTGCAG 1 cut(s) 1971
PstNI CAGNNNCTG 3 cut(s) 763, 1115, 1738
PvuII CAGCTG 2 cut(s) 728, 1112
RsaI GTAC 4 cut(s) 762, 1076, 1430, 1517
RsaNI GTAC 4 cut(s) 761, 1075, 1429, 1516
RseI CAYNNNNRTG 2 cut(s) 102, 1002
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 5 cut(s) 414, 816, 842, 1623, 1902
SatI GCNGC 7 cut(s) 441, 726, 983, 1062, 1113, 1226, 1739
Sau96I GGNCC 2 cut(s) 295, 1304
SbfI CCTGCAGG 1 cut(s) 1971
SchI GAGTC 2 cut(s) 575, 623
ScrFI CCNGG 3 cut(s) 1664, 1801, 1883
SdaI CCTGCAGG 1 cut(s) 1971
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 2 cut(s) 231, 1272
SfcI CTRYAG 2 cut(s) 1500, 1967
SinI GGWCC 1 cut(s) 1304
SmiMI CAYNNNNRTG 2 cut(s) 102, 1002
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
SphI GCATGC 2 cut(s) 280, 1155
Sse8387I CCTGCAGG 1 cut(s) 1971
Sse9I AATT 5 cut(s) 8, 28, 1334, 1432, 1458
SseBI AGGCCT 1 cut(s) 1816
SsiI CCGC 4 cut(s) 441, 714, 1064, 1387
SspI AATATT 1 cut(s) 1909
SspMI CTAG 6 cut(s) 332, 444, 491, 1205, 1391, 1851
SstI GAGCTC 1 cut(s) 188
StuI AGGCCT 1 cut(s) 1816
StyD4I CCNGG 3 cut(s) 1662, 1799, 1881
StyI CCWWGG 4 cut(s) 602, 997, 1559, 1612
TaaI ACNGT 7 cut(s) 290, 1359, 1501, 1546, 1556, 1570, 1659
TaqI TCGA 4 cut(s) 353, 1044, 1236, 1354
TasI AATT 5 cut(s) 8, 28, 1334, 1432, 1458
TatI WGTACW 3 cut(s) 1074, 1428, 1515
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 7 cut(s) 86, 161, 494, 731, 803, 1410, 1590
Tru1I TTAA 5 cut(s) 414, 816, 842, 1623, 1902
Tru9I TTAA 5 cut(s) 414, 816, 842, 1623, 1902
TscAI CASTG 5 cut(s) 118, 313, 442, 671, 1838
TseI GCWGC 6 cut(s) 725, 982, 1061, 1112, 1225, 1738
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 5 cut(s) 118, 313, 442, 671, 1838
Van91I CCANNNNNTGG 1 cut(s) 1618
VpaK11BI GGWCC 1 cut(s) 1304
XapI RAATTY 2 cut(s) 8, 1334
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 4 cut(s) 280, 1155, 1791, 1997
XmnI GAANNNNTTC 2 cut(s) 29, 498
XspI CTAG 6 cut(s) 332, 444, 491, 1205, 1391, 1851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.