Rh5BG448300

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000175
Physical Location & Seq
Reverse (-)
71875063 .. 71882698
7636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG448300.1

Sequence Viewer

Length: 1242 bp
ATGCCAACATCTCCAGATCAGAATATCATTTTTACTGAGTTTGATCAACACAATCTTGAAATTACTGATCCCAGTTCGTTTCTATCCAATCCAAACCCTCCTCCTCGTGATGTTTCATCCCGGTCTTTGGGTGTGACCTCAGAGGCCTCCAGAGCTACTCCCCAGTATATAAGTGAGATGCTTATGGAGGAAGAGCTGGAGAACAGACCCTGCATGCTTCAGGACTGTTTGGCCCTCCAAGCTGCTGAGAAATCCTTGGAAGATGTCCTGGTTCAGCAATATCCTCCTTCGACTAATCCCATTCTTGCTTCTTCCATTCAGCAAAATGTTGACAACTCTGATGATTTCATTAACCACAGCAGTAATAGCTCCACTGCTGCTAGAAACTGGCTTAAAGGGGGAAGGGGACATGCAGGATCCAAAGGCAAAAGAACAGTTAAGAAAAAAAAGGATGACAACACTGAAGTAGTGGATTTTCAGTCACTGCTAACTCAGTGTGCAAAGGCAAGCTATGACAGAAGGACTGCAAATGAACAACTGAAACAGATAAGGCAGCACTCATCTCCCTACGGTGATGGAACCCAAAGAGTAGCTCATTACTTAGCCAATGGCCTTGAAGAACACTTGGCTGCTGCAGTTCCTTCGTTCAATCCTCTTTGCCTTAATAAGATGTCAGCTGCTGATATCTTAAAAGCTTACCAGACATATATCAAAGCATGCCCCTTCAAGTTGATGTCAAACATCTATGCTAACAAAACTATCTTCAAACTAACAGAGAAAGCAACAAGGCTTCACATAATTGATTTTGGTATTCTCTATGGCTACCAATGGCCTGGCCTTATCCAAAGTCTTGCTAAAAGACCCTCTGGACCTCCCATGCTTCGCATTACTGGTATTGAATTTCCACAATCAGGATTTCGACCTTCAGAGAGTCTTGAAGAGACAGGGCACCTCCTAGCGAAATATTGCAAGAGATTCAATGTCCCATTTGAGTACAACTTCATAGCACAGGATTGGGAAACCATTCGATATCAAGAGATCAAACTTGACAGAGATGAGTTTACTGTAGTGAACTGCTTGTGCAGATTAAGGAACGTACCTGAAGAAACAGAGATGAGGAGTCCAAGGGATAGAGTTTTGAAGCTGATCAGGAGAATCAACCCAGATATGTATATCCTTGGACTGGTTAATGGAACCTATAATGCACCCTTCTTCAACATACGGTTCCGGGAGGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

413

Amino Acids

46.94

Weight (kDa)

7.58

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 160 - 413 8.5e-73 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 948
AclWI GGATC 3 cut(s) 62, 411, 424
AcsI RAATTY 1 cut(s) 899
AcuI CTGAAG 4 cut(s) 203, 483, 909, 1122
AfaI GTAC 2 cut(s) 995, 1098
AfiI CCNNNNNNNGG 3 cut(s) 127, 911, 1183
AjnI CCWGG 2 cut(s) 267, 832
AluBI AGCT 9 cut(s) 155, 196, 242, 369, 510, 593, 677, 695, 1144
AluI AGCT 9 cut(s) 155, 196, 242, 369, 510, 593, 677, 695, 1144
Alw26I GTCTC 1 cut(s) 935
AlwI GGATC 3 cut(s) 62, 411, 424
AlwNI CAGNNNCTG 3 cut(s) 210, 484, 680
AoxI GGCC 5 cut(s) 144, 231, 610, 830, 835
ApeKI GCWGC 6 cut(s) 242, 377, 553, 629, 632, 677
ApoI RAATTY 1 cut(s) 899
Asp700I GAANNNNTTC 1 cut(s) 1023
AspS9I GGNCC 2 cut(s) 232, 869
AsuC2I CCSGG 2 cut(s) 121, 1229
AsuHPI GGTGA 1 cut(s) 584
AvaII GGWCC 1 cut(s) 869
BaeGI GKGCMC 1 cut(s) 951
BamHI GGATCC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 948
BauI CACGAG 1 cut(s) 105
BbvI GCAGC 6 cut(s) 229, 364, 565, 616, 619, 664
BccI CCATC 1 cut(s) 569
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BciT130I CCWGG 2 cut(s) 269, 834
BclI TGATCA 2 cut(s) 43, 1146
BcnI CCSGG 2 cut(s) 121, 1229
BcoDI GTCTC 1 cut(s) 935
BfaI CTAG 3 cut(s) 381, 956, 1240
BfmI CTRYAG 2 cut(s) 633, 1065
BisI GCNGC 6 cut(s) 243, 378, 554, 630, 633, 678
BlsI GCNGC 6 cut(s) 244, 379, 555, 631, 634, 679
Bme1390I CCNGG 4 cut(s) 121, 269, 834, 1229
Bme18I GGWCC 1 cut(s) 869
BmgT120I GGNCC 2 cut(s) 232, 869
BmiI GGNNCC 5 cut(s) 418, 580, 950, 1195, 1226
BmrFI CCNGG 4 cut(s) 121, 269, 834, 1229
BmrI ACTGGG 2 cut(s) 66, 157
BmsI GCATC 1 cut(s) 168
BmuI ACTGGG 2 cut(s) 66, 157
BpmI CTGGAG 2 cut(s) 133, 218
BpuMI CCSGG 2 cut(s) 121, 1229
BsaJI CCNNGG 3 cut(s) 255, 1124, 1177
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 911, 1183
Bse1I ACTGG 5 cut(s) 72, 163, 392, 895, 1188
BseBI CCWGG 2 cut(s) 269, 834
BseDI CCNNGG 3 cut(s) 255, 1124, 1177
BseGI GGATG 2 cut(s) 116, 457
BseLI CCNNNNNNNGG 3 cut(s) 127, 911, 1183
BseMII CTCAG 4 cut(s) 27, 153, 237, 506
BseNI ACTGG 5 cut(s) 72, 163, 392, 895, 1188
BseRI GAGGAG 3 cut(s) 90, 93, 1132
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 6 cut(s) 229, 364, 565, 616, 619, 664
BsgI GTGCAG 1 cut(s) 1102
BshFI GGCC 5 cut(s) 146, 233, 612, 832, 837
BshNI GGYRCC 1 cut(s) 948
BsiSI CCGG 2 cut(s) 121, 1228
BslFI GGGAC 2 cut(s) 420, 968
BslI CCNNNNNNNGG 3 cut(s) 127, 911, 1183
BsmAI GTCTC 1 cut(s) 935
BsmFI GGGAC 2 cut(s) 420, 968
BsnI GGCC 5 cut(s) 146, 233, 612, 832, 837
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
BspANI GGCC 5 cut(s) 146, 233, 612, 832, 837
BspCNI CTCAG 4 cut(s) 28, 152, 238, 505
BspLI GGNNCC 5 cut(s) 418, 580, 950, 1195, 1226
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 3 cut(s) 62, 411, 424
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 948
BsrI ACTGG 5 cut(s) 72, 163, 392, 895, 1188
BssECI CCNNGG 3 cut(s) 255, 1124, 1177
BssMI GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 3 cut(s) 255, 1124, 1177
Bst2BI CACGAG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 269, 834
Bst4CI ACNGT 5 cut(s) 227, 436, 572, 1066, 1224
Bst6I CTCTTC 2 cut(s) 186, 933
BstC8I GCNNGC 3 cut(s) 215, 508, 718
BstDEI CTNAG 5 cut(s) 36, 139, 246, 492, 601
BstF5I GGATG 2 cut(s) 116, 457
BstKTI GATC 6 cut(s) 19, 46, 70, 419, 1041, 1149
BstMAI GTCTC 1 cut(s) 935
BstMBI GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
BstMWI GCNNNNNNNGC 3 cut(s) 152, 239, 366
BstNI CCWGG 2 cut(s) 269, 834
BstNSI RCATGY 3 cut(s) 217, 413, 720
BstSCI CCNGG 4 cut(s) 119, 267, 832, 1227
BstSFI CTRYAG 2 cut(s) 633, 1065
BstSLI GKGCMC 1 cut(s) 951
BstV1I GCAGC 6 cut(s) 229, 364, 565, 616, 619, 664
BstX2I RGATCY 1 cut(s) 416
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 5 cut(s) 146, 233, 612, 832, 837
BtsCI GGATG 2 cut(s) 116, 457
BtsI GCAGTG 2 cut(s) 372, 482
BtsIMutI CAGTG 4 cut(s) 372, 459, 482, 500
Cac8I GCNNGC 3 cut(s) 215, 508, 718
CaiI CAGNNNCTG 3 cut(s) 210, 484, 680
Cfr13I GGNCC 2 cut(s) 232, 869
Csp6I GTAC 2 cut(s) 994, 1097
CviAII CATG 4 cut(s) 214, 410, 717, 877
CviQI GTAC 2 cut(s) 994, 1097
DdeI CTNAG 5 cut(s) 36, 139, 246, 492, 601
DpnI GATC 6 cut(s) 18, 45, 69, 418, 1040, 1148
DpnII GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
Eam1104I CTCTTC 2 cut(s) 186, 933
EarI CTCTTC 2 cut(s) 186, 933
Eco130I CCWWGG 3 cut(s) 255, 1124, 1177
Eco147I AGGCCT 1 cut(s) 146
Eco32I GATATC 2 cut(s) 685, 1031
Eco47I GGWCC 1 cut(s) 869
Eco57I CTGAAG 4 cut(s) 203, 483, 909, 1122
EcoRII CCWGG 2 cut(s) 267, 832
EcoRV GATATC 2 cut(s) 685, 1031
EcoT14I CCWWGG 3 cut(s) 255, 1124, 1177
ErhI CCWWGG 3 cut(s) 255, 1124, 1177
FaeI CATG 4 cut(s) 217, 413, 720, 880
FaqI GGGAC 2 cut(s) 420, 968
FatI CATG 4 cut(s) 213, 409, 716, 876
FbaI TGATCA 2 cut(s) 43, 1146
Fnu4HI GCNGC 6 cut(s) 243, 378, 554, 630, 633, 678
FokI GGATG 2 cut(s) 103, 464
Fsp4HI GCNGC 6 cut(s) 243, 378, 554, 630, 633, 678
FspBI CTAG 3 cut(s) 381, 956, 1240
GluI GCNGC 6 cut(s) 243, 378, 554, 630, 633, 678
GsuI CTGGAG 2 cut(s) 133, 218
HaeIII GGCC 5 cut(s) 146, 233, 612, 832, 837
HapII CCGG 2 cut(s) 121, 1228
Hin1II CATG 4 cut(s) 217, 413, 720, 880
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 4 cut(s) 931, 975, 1120, 1155
HpaII CCGG 2 cut(s) 121, 1228
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 3 cut(s) 331, 1062, 1072
Hpy188I TCNGA 4 cut(s) 21, 142, 340, 928
Hpy8I GTNNAC 3 cut(s) 331, 1062, 1072
HpyAV CCTTC 7 cut(s) 297, 396, 513, 651, 733, 933, 1219
HpyCH4III ACNGT 5 cut(s) 227, 436, 572, 1066, 1224
HpyCH4IV ACGT 1 cut(s) 1095
HpyCH4V TGCA 8 cut(s) 213, 413, 500, 527, 635, 969, 1083, 1205
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 239, 366
HpyF3I CTNAG 5 cut(s) 36, 139, 246, 492, 601
HpySE526I ACGT 1 cut(s) 1095
Hsp92II CATG 4 cut(s) 217, 413, 720, 880
Ksp22I TGATCA 2 cut(s) 43, 1146
Kzo9I GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 1 cut(s) 374
Lsp1109I GCAGC 6 cut(s) 229, 364, 565, 616, 619, 664
LweI GCATC 1 cut(s) 168
MaeI CTAG 3 cut(s) 381, 956, 1240
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 2 cut(s) 133, 480
MalI GATC 6 cut(s) 18, 45, 69, 418, 1040, 1148
MboI GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
MboII GAAGA 8 cut(s) 203, 272, 303, 629, 754, 950, 1115, 1204
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 1 cut(s) 951
MluCI AATT 3 cut(s) 60, 798, 899
MlyI GAGTC 2 cut(s) 940, 1129
MroXI GAANNNNTTC 1 cut(s) 1023
MseI TTAA 7 cut(s) 351, 393, 438, 663, 689, 1088, 1188
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 2 cut(s) 121, 1228
MspR9I CCNGG 4 cut(s) 121, 269, 834, 1229
MvaI CCWGG 2 cut(s) 269, 834
MwoI GCNNNNNNNGC 3 cut(s) 152, 239, 366
NciI CCSGG 2 cut(s) 121, 1229
NdeII GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
NlaIII CATG 4 cut(s) 217, 413, 720, 880
NlaIV GGNNCC 5 cut(s) 418, 580, 950, 1195, 1226
NmuCI GTSAC 2 cut(s) 133, 480
NspI RCATGY 3 cut(s) 217, 413, 720
PaeI GCATGC 2 cut(s) 217, 720
PceI AGGCCT 1 cut(s) 146
PciSI GCTCTTC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 2 cut(s) 975, 1155
PfoI TCCNGGA 1 cut(s) 1227
PkrI GCNGC 6 cut(s) 244, 379, 555, 631, 634, 679
PleI GAGTC 2 cut(s) 939, 1128
PpsI GAGTC 2 cut(s) 939, 1128
Psp6I CCWGG 2 cut(s) 267, 832
PspGI CCWGG 2 cut(s) 267, 832
PspN4I GGNNCC 5 cut(s) 418, 580, 950, 1195, 1226
PspPI GGNCC 2 cut(s) 232, 869
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 3 cut(s) 210, 484, 680
PsuI RGATCY 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 995, 1098
RsaNI GTAC 2 cut(s) 994, 1097
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 7 cut(s) 351, 393, 438, 663, 689, 1088, 1188
SatI GCNGC 6 cut(s) 243, 378, 554, 630, 633, 678
Sau3AI GATC 6 cut(s) 16, 43, 67, 416, 1038, 1146
Sau96I GGNCC 2 cut(s) 232, 869
SchI GAGTC 2 cut(s) 940, 1129
ScrFI CCNGG 4 cut(s) 121, 269, 834, 1229
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 2 cut(s) 633, 1065
SinI GGWCC 1 cut(s) 869
SphI GCATGC 2 cut(s) 217, 720
Sse9I AATT 3 cut(s) 60, 798, 899
SseBI AGGCCT 1 cut(s) 146
SspI AATATT 1 cut(s) 965
SspMI CTAG 3 cut(s) 381, 956, 1240
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 4 cut(s) 119, 267, 832, 1227
StyI CCWWGG 3 cut(s) 255, 1124, 1177
TaaI ACNGT 5 cut(s) 227, 436, 572, 1066, 1224
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 3 cut(s) 290, 919, 1027
TasI AATT 3 cut(s) 60, 798, 899
TatI WGTACW 1 cut(s) 993
TfiI GAWTC 2 cut(s) 975, 1155
Tru1I TTAA 7 cut(s) 351, 393, 438, 663, 689, 1088, 1188
Tru9I TTAA 7 cut(s) 351, 393, 438, 663, 689, 1088, 1188
TscAI CASTG 4 cut(s) 379, 466, 489, 500
TseFI GTSAC 2 cut(s) 133, 480
TseI GCWGC 6 cut(s) 242, 377, 553, 629, 632, 677
Tsp45I GTSAC 2 cut(s) 133, 480
TspDTI ATGAA 4 cut(s) 105, 337, 546, 991
TspRI CASTG 4 cut(s) 379, 466, 489, 500
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 1 cut(s) 899
XceI RCATGY 3 cut(s) 217, 413, 720
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 3 cut(s) 381, 956, 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.