MD03G1089100.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000175
Physical Location & Seq
Reverse (-)
7337571 .. 7339625
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1089100.v1.1.491

Sequence Viewer

Length: 2055 bp
ATGTATAACCTTTATGAAAGATTCTCAATTTCCATGAAAAACTTCCAGTTTGGTTCTACCCAGAATCTTGTCAATGGATTCCAAGTGAATCATGAACCCACCAATCCACTTTTCCTTCCAACTACCACAGACCATCCAACCGATTCCGGGCTTTCGAGCTCGGGATCAGATGGAGATTGCTTTGACATTAGTGACAGCCGTCCTGTGCTCAAGTACATAAGTGATATTCTTTTGGAGGAGGACTTGGAGGATAAGCCCTGCATGTTGCAGAACTGTTTGGCCCTCCTTGCTGCTGAGAAATCTTTCTATGATGTTCTTAATGCGAAGGATCCTCCTTCGCCTGACCAACCGCCTCTTCAGGTGTACCATAGCTTTGAGCACTCAGATGATGATTCCTCACATAGTTGCCATAGCAGTAATGATTCTAGATCTGCTAGGACAGACTCGGTTTTCGATTGTTCAGAAACCCTGTTGGCTTCAGACTCAATTTCTGGGAATTTTGGAGGAGTTGGGGAAGCAAGAACTCTCATTCCAAATGGGAGATATGGAATCATTGACGTGGAGAGGTACCAATCAGTCCCCACGGGAGGTAATACACTGAGGAGGAATTTTGCACCCGAGCCAGAGAATGATGGCTACATCTCAGCAAACAGATTGAAGGGAAAGAAGAATCGTCAAAGGGAGGATGGTGATTATGCAGATGAAGGGAGAAGTAGCAAGCAATCAGCTGCTTTAGGCGACGATGCTGAGCCACAAGAAATGTTTGATAAGGTATTACTCTGTCATGTGAATCATACGCATGAATCTTGTATTCGTGACAAACCTTTGGGCAGTGAGGTATGTGAGAAGTTGCAGCCTACCAAGCAGTCAAAAGGGTCTAAAACAACCCGCTCGAAGAAACAGAACAATAACAGGGAAGTTGTGGATCTTACCACCCTGCTAACTCAATGTGCACAAGCCGTGGCAAGCTATGACCAACGAACTGCGAGTGAACTACTGAAGCAGATAAGGAAGCACTCTTCTCCCTATGGTGATGCAACCGAGAGATTATCTCATTACTTTGCTGATGGCCTTGAGGCGCGCTTGGCAGGAGCCAGAACCCCGTTATATTCGCCTCTTTTGAGTATACAGACACCAGTTGCTGAAATCTTAAAAGCCTACCAGATGTATGTTAAGTATTGCCCTTTCAAGCACATGTTACACTTCTTTTCCAACAGGACAATTATTAAACTAGCTGAAAAGGCAACAAGGCTTCACGTTGTTGATTTTGGTATTTCCTATGGTTTCCAATGGCCCTGCTTTATCCAACGCCTCTCAGAGAGAAATGGTGGACCTCCTCATATTCGTTTGACAGCAATTGAGCTTCCCCAACCAGGTTTTCTACCTACAGAAAGGGTTGAAGAGACGGGGCGTCGCTTGAAAAAATATGCTGCAAGATTTAATGTCCAGTTTGAGTACAAGGTCATTGCACGGAAATGGGAGACTATCCAACTTGAGGATCTTAAAATTGACAGAAATGAGCTAACGGTGGTGAACTGCATGCACCGGTTAAAGCACATACCTGATGAAACCGTGGTGGTAAGCAGTCCTAGGGACATTGTCCTAAAGTTGATCAGAAAAATTAATCCAGACCTATTTATTCACGGAGTCATCAACGGGACATACAATTCACCCTTCTTTGTCACACGGTTCAAAGAGGCGCTCTACCACTTCTCTGCAATGTTTGACATGTTTGAGGCCACTATTCCTCGAGAAGATGAGCAGCGGCTGATGTTTGAAAAGGCAGTATATGGTAAAGACATAATGAATGTCGTCGCGTGTGAAGGACTGGAACGAGTTGAAAGGCCTGAGACATACAAGCAGTGGCAGGTTAGGTATCATCGAGCTGGGTTCAAGCAGGTGCCTTTAGACCAGGGACTCTTGAAGAGAGTGAAGATTATGCTGAAGGCAATGGATTATCATGATGATTTTCGAATAGATGAAGATGGCGAGTGGATGCTTCAGGGATGGAAAGGCAGAATCATCTTCGGTCTTGCGTTCTGGAAACCTGCTTAG

Protein Analysis

685

Amino Acids

78.08

Weight (kDa)

6.42

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 311 - 682 3e-121 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1888
Acc36I ACCTGC 2 cut(s) 1858, 1888
Acc65I GGTACC 1 cut(s) 567
AccB1I GGYRCC 2 cut(s) 567, 1900
AccBSI CCGCTC 1 cut(s) 891
AccI GTMKAC 1 cut(s) 1126
AccII CGCG 2 cut(s) 1081, 1817
AciI CCGC 3 cut(s) 350, 889, 1765
AclWI GGATC 5 cut(s) 172, 323, 336, 933, 1506
AcsI RAATTY 2 cut(s) 496, 607
AcuI CTGAAG 5 cut(s) 341, 462, 1019, 1964, 1985
AcyI GRCGYC 1 cut(s) 1411
AfaI GTAC 4 cut(s) 215, 365, 569, 1457
AfiI CCNNNNNNNGG 4 cut(s) 147, 587, 1373, 1495
AflIII ACRYGT 2 cut(s) 1194, 1728
AgeI ACCGGT 1 cut(s) 1545
AgsI TTSAA 9 cut(s) 658, 1189, 1400, 1420, 1693, 1778, 1841, 1894, 1924
AhdI GACNNNNNGTC 1 cut(s) 1410
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 1372, 1911
AjuI GAANNNNNNNTTGG 6 cut(s) 95, 127, 339, 371, 1362, 1394
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 8 cut(s) 159, 372, 728, 969, 1235, 1363, 1522, 1886
AluI AGCT 8 cut(s) 159, 372, 728, 969, 1235, 1363, 1522, 1886
Alw21I GWGCWC 4 cut(s) 161, 210, 381, 955
Alw26I GTCTC 3 cut(s) 1397, 1475, 1844
Alw44I GTGCAC 1 cut(s) 951
AlwI GGATC 5 cut(s) 172, 323, 336, 933, 1506
AlwNI CAGNNNCTG 2 cut(s) 1142, 1768
Ama87I CYCGRG 3 cut(s) 160, 617, 1749
AoxI GGCC 5 cut(s) 279, 1069, 1292, 1737, 1844
ApaLI GTGCAC 1 cut(s) 951
ApeKI GCWGC 5 cut(s) 290, 728, 853, 1430, 1762
ApoI RAATTY 2 cut(s) 496, 607
ArsI GACNNNNNNTTYG 2 cut(s) 434, 466
AseI ATTAAT 1 cut(s) 1623
AsiGI ACCGGT 1 cut(s) 1545
Asp700I GAANNNNTTC 2 cut(s) 41, 302
Asp718I GGTACC 1 cut(s) 567
AspA2I CCTAGG 1 cut(s) 1589
AspLEI GCGC 3 cut(s) 1081, 1083, 1702
AspS9I GGNCC 3 cut(s) 280, 1293, 1331
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 4 cut(s) 701, 1043, 1543, 1662
AsuII TTCGAA 1 cut(s) 1972
AvaI CYCGRG 3 cut(s) 160, 617, 1749
AvaII GGWCC 1 cut(s) 1331
AvrII CCTAGG 1 cut(s) 1589
BaeGI GKGCMC 1 cut(s) 955
BamHI GGATCC 1 cut(s) 328
BanI GGYRCC 2 cut(s) 567, 1900
BanII GRGCYC 1 cut(s) 161
Bbv12I GWGCWC 4 cut(s) 161, 210, 381, 955
BbvI GCAGC 5 cut(s) 277, 715, 865, 1417, 1774
BccI CCATC 7 cut(s) 141, 164, 626, 680, 1061, 1979, 2001
BceAI ACGGC 2 cut(s) 183, 944
BciT130I CCWGG 2 cut(s) 1374, 1913
BclI TGATCA 1 cut(s) 1611
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 3 cut(s) 1397, 1475, 1844
BfaI CTAG 4 cut(s) 426, 435, 1232, 1590
BfmI CTRYAG 1 cut(s) 1386
BfoI RGCGCY 1 cut(s) 1703
BfuAI ACCTGC 2 cut(s) 1858, 1888
BglII AGATCT 1 cut(s) 428
BisI GCNGC 6 cut(s) 291, 729, 854, 1431, 1763, 1766
BlnI CCTAGG 1 cut(s) 1589
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 6 cut(s) 292, 730, 855, 1432, 1764, 1767
Bme1390I CCNGG 3 cut(s) 148, 1374, 1913
Bme18I GGWCC 1 cut(s) 1331
BmeRI GACNNNNNGTC 1 cut(s) 1410
BmeT110I CYCGRG 3 cut(s) 160, 617, 1749
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 3 cut(s) 280, 1293, 1331
BmiI GGNNCC 4 cut(s) 330, 569, 1093, 1902
BmrFI CCNGG 3 cut(s) 148, 1374, 1913
BmsI GCATC 3 cut(s) 733, 1024, 1986
BoxI GACNNNNGTC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 1036, 1068
Bpu1102I GCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 1972
BpuEI CTTGAG 3 cut(s) 194, 1094, 1514
BpuMI CCSGG 1 cut(s) 148
BsaHI GRCGYC 1 cut(s) 1411
BsaJI CCNNGG 5 cut(s) 582, 960, 1572, 1589, 1912
BsaWI WCCGGW 1 cut(s) 1545
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 587, 1373, 1495
Bse118I RCCGGY 1 cut(s) 1545
Bse1I ACTGG 4 cut(s) 46, 1136, 1447, 1833
Bse3DI GCAATG 3 cut(s) 1464, 1725, 1956
BseBI CCWGG 2 cut(s) 1374, 1913
BseDI CCNNGG 5 cut(s) 582, 960, 1572, 1589, 1912
BseGI GGATG 4 cut(s) 133, 691, 2001, 2012
BseLI CCNNNNNNNGG 4 cut(s) 147, 587, 1373, 1495
BseMI GCAATG 3 cut(s) 1464, 1725, 1956
BseMII CTCAG 7 cut(s) 285, 396, 590, 657, 738, 1329, 1839
BseNI ACTGG 4 cut(s) 46, 1136, 1447, 1833
BsePI GCGCGC 1 cut(s) 1079
BseRI GAGGAG 4 cut(s) 251, 519, 616, 1326
BseSI GKGCMC 1 cut(s) 955
BseXI GCAGC 5 cut(s) 277, 715, 865, 1417, 1774
BseYI CCCAGC 1 cut(s) 1886
Bsh1236I CGCG 2 cut(s) 1081, 1817
BshFI GGCC 5 cut(s) 281, 1071, 1294, 1739, 1846
BshNI GGYRCC 2 cut(s) 567, 1900
BshTI ACCGGT 1 cut(s) 1545
BsiHKAI GWGCWC 4 cut(s) 161, 210, 381, 955
BsiHKCI CYCGRG 3 cut(s) 160, 617, 1749
BsiSI CCGG 2 cut(s) 147, 1546
BslFI GGGAC 4 cut(s) 563, 1607, 1672, 1929
BslI CCNNNNNNNGG 4 cut(s) 147, 587, 1373, 1495
BsmAI GTCTC 3 cut(s) 1397, 1475, 1844
BsmBI CGTCTC 1 cut(s) 1397
BsmFI GGGAC 4 cut(s) 563, 1607, 1672, 1929
BsnI GGCC 5 cut(s) 281, 1071, 1294, 1739, 1846
BsoBI CYCGRG 3 cut(s) 160, 617, 1749
Bsp119I TTCGAA 1 cut(s) 1972
Bsp1286I GDGCHC 4 cut(s) 161, 210, 381, 955
Bsp143I GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
Bsp1720I GCTNAGC 1 cut(s) 747
BspACI CCGC 3 cut(s) 350, 889, 1765
BspANI GGCC 5 cut(s) 281, 1071, 1294, 1739, 1846
BspCNI CTCAG 7 cut(s) 286, 395, 591, 656, 739, 1328, 1840
BspFNI CGCG 2 cut(s) 1081, 1817
BspHI TCATGA 2 cut(s) 91, 1960
BspLI GGNNCC 4 cut(s) 330, 569, 1093, 1902
BspMI ACCTGC 2 cut(s) 1858, 1888
BspPI GGATC 5 cut(s) 172, 323, 336, 933, 1506
BspT104I TTCGAA 1 cut(s) 1972
BspT107I GGYRCC 2 cut(s) 567, 1900
BsrBI CCGCTC 1 cut(s) 891
BsrDI GCAATG 3 cut(s) 1464, 1725, 1956
BsrFI RCCGGY 1 cut(s) 1545
BsrI ACTGG 4 cut(s) 46, 1136, 1447, 1833
BssAI RCCGGY 1 cut(s) 1545
BssECI CCNNGG 5 cut(s) 582, 960, 1572, 1589, 1912
BssHII GCGCGC 1 cut(s) 1079
BssMI GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
BssNAI GTATAC 1 cut(s) 1127
BssNI GRCGYC 1 cut(s) 1411
BssT1I CCWWGG 1 cut(s) 1589
Bst1107I GTATAC 1 cut(s) 1127
Bst2UI CCWGG 2 cut(s) 1374, 1913
Bst4CI ACNGT 4 cut(s) 275, 1528, 1573, 1689
Bst6I CTCTTC 4 cut(s) 360, 1024, 1395, 1919
BstACI GRCGYC 1 cut(s) 1411
BstBI TTCGAA 1 cut(s) 1972
BstC8I GCNNGC 4 cut(s) 719, 967, 1081, 1541
BstDEI CTNAG 8 cut(s) 294, 382, 599, 643, 747, 1315, 1848, 2052
BstDSI CCRYGG 3 cut(s) 582, 960, 1572
BstF5I GGATG 4 cut(s) 133, 691, 2001, 2012
BstFNI CGCG 2 cut(s) 1081, 1817
BstH2I RGCGCY 1 cut(s) 1703
BstHHI GCGC 3 cut(s) 1081, 1083, 1702
BstKTI GATC 6 cut(s) 167, 331, 431, 928, 1501, 1614
BstMAI GTCTC 3 cut(s) 1397, 1475, 1844
BstMBI GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
BstMWI GCNNNNNNNGC 4 cut(s) 287, 862, 1085, 1241
BstNI CCWGG 2 cut(s) 1374, 1913
BstNSI RCATGY 4 cut(s) 265, 1198, 1543, 1732
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 3 cut(s) 146, 1372, 1911
BstSFI CTRYAG 1 cut(s) 1386
BstSLI GKGCMC 1 cut(s) 955
BstUI CGCG 2 cut(s) 1081, 1817
BstV1I GCAGC 5 cut(s) 277, 715, 865, 1417, 1774
BstX2I RGATCY 4 cut(s) 328, 428, 925, 1498
BstYI RGATCY 4 cut(s) 328, 428, 925, 1498
BstZ17I GTATAC 1 cut(s) 1127
BsuRI GGCC 5 cut(s) 281, 1071, 1294, 1739, 1846
BtgI CCRYGG 3 cut(s) 582, 960, 1572
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 4 cut(s) 133, 691, 2001, 2012
BtsI GCAGTG 2 cut(s) 838, 1868
BtsIMutI CAGTG 3 cut(s) 596, 838, 1868
BveI ACCTGC 2 cut(s) 1858, 1888
Cac8I GCNNGC 4 cut(s) 719, 967, 1081, 1541
CaiI CAGNNNCTG 2 cut(s) 1142, 1768
CciI TCATGA 2 cut(s) 91, 1960
CfoI GCGC 3 cut(s) 1081, 1083, 1702
Cfr10I RCCGGY 1 cut(s) 1545
Cfr13I GGNCC 3 cut(s) 280, 1293, 1331
CseI GACGC 1 cut(s) 1400
CsiI ACCWGGT 1 cut(s) 1372
Csp6I GTAC 4 cut(s) 214, 364, 568, 1456
CspAI ACCGGT 1 cut(s) 1545
CviAII CATG 9 cut(s) 34, 92, 262, 785, 800, 1195, 1540, 1729, 1961
CviQI GTAC 4 cut(s) 214, 364, 568, 1456
DdeI CTNAG 8 cut(s) 294, 382, 599, 643, 747, 1315, 1848, 2052
DpnI GATC 6 cut(s) 166, 330, 430, 927, 1500, 1613
DpnII GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
DriI GACNNNNNGTC 1 cut(s) 1410
Eam1104I CTCTTC 4 cut(s) 360, 1024, 1395, 1919
Eam1105I GACNNNNNGTC 1 cut(s) 1410
EarI CTCTTC 4 cut(s) 360, 1024, 1395, 1919
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 1589
Eco147I AGGCCT 1 cut(s) 1846
Eco24I GRGCYC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 1331
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 5 cut(s) 341, 462, 1019, 1964, 1985
Eco88I CYCGRG 3 cut(s) 160, 617, 1749
EcoICRI GAGCTC 1 cut(s) 159
EcoRII CCWGG 2 cut(s) 1372, 1911
EcoT14I CCWWGG 1 cut(s) 1589
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 1589
Esp3I CGTCTC 1 cut(s) 1397
FaeI CATG 9 cut(s) 37, 95, 265, 788, 803, 1198, 1543, 1732, 1964
FaqI GGGAC 4 cut(s) 563, 1607, 1672, 1929
FatI CATG 9 cut(s) 33, 91, 261, 784, 799, 1194, 1539, 1728, 1960
FauI CCCGC 1 cut(s) 896
FbaI TGATCA 1 cut(s) 1611
FblI GTMKAC 1 cut(s) 1126
Fnu4HI GCNGC 6 cut(s) 291, 729, 854, 1431, 1763, 1766
FokI GGATG 4 cut(s) 120, 698, 2008, 2019
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 6 cut(s) 291, 729, 854, 1431, 1763, 1766
FspBI CTAG 4 cut(s) 426, 435, 1232, 1590
GlaI GCGC 3 cut(s) 1080, 1082, 1701
GluI GCNGC 6 cut(s) 291, 729, 854, 1431, 1763, 1766
GsaI CCCAGC 1 cut(s) 1890
HaeII RGCGCY 1 cut(s) 1703
HaeIII GGCC 5 cut(s) 281, 1071, 1294, 1739, 1846
HapII CCGG 2 cut(s) 147, 1546
HgaI GACGC 1 cut(s) 1400
HhaI GCGC 3 cut(s) 1081, 1083, 1702
Hin1I GRCGYC 1 cut(s) 1411
Hin1II CATG 9 cut(s) 37, 95, 265, 788, 803, 1198, 1543, 1732, 1964
Hin6I GCGC 3 cut(s) 1079, 1081, 1700
HinP1I GCGC 3 cut(s) 1079, 1081, 1700
HpaII CCGG 2 cut(s) 147, 1546
HphI GGTGA 4 cut(s) 701, 1043, 1543, 1662
Hpy166II GTNNAC 6 cut(s) 364, 953, 992, 1127, 1331, 1534
Hpy188I TCNGA 6 cut(s) 169, 385, 463, 481, 1318, 1616
Hpy188III TCNNGA 9 cut(s) 92, 162, 426, 815, 1628, 1751, 1921, 1961, 2041
Hpy8I GTNNAC 6 cut(s) 364, 953, 992, 1127, 1331, 1534
Hpy99I CGWCG 3 cut(s) 743, 1416, 1817
HpyAV CCTTC 8 cut(s) 125, 319, 345, 652, 698, 1684, 1817, 1939
HpyCH4III ACNGT 4 cut(s) 275, 1528, 1573, 1689
HpyCH4IV ACGT 2 cut(s) 558, 1257
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 862, 1085, 1241
HpyF3I CTNAG 8 cut(s) 294, 382, 599, 643, 747, 1315, 1848, 2052
HpySE526I ACGT 2 cut(s) 558, 1257
Hsp92I GRCGYC 1 cut(s) 1411
Hsp92II CATG 9 cut(s) 37, 95, 265, 788, 803, 1198, 1543, 1732, 1964
HspAI GCGC 3 cut(s) 1079, 1081, 1700
KpnI GGTACC 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 1611
Kzo9I GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
LmnI GCTCC 1 cut(s) 1091
Lsp1109I GCAGC 5 cut(s) 277, 715, 865, 1417, 1774
LweI GCATC 3 cut(s) 733, 1024, 1986
MabI ACCWGGT 1 cut(s) 1372
MaeI CTAG 4 cut(s) 426, 435, 1232, 1590
MaeII ACGT 2 cut(s) 558, 1257
MaeIII GTNAC 4 cut(s) 191, 815, 1197, 1681
MalI GATC 6 cut(s) 166, 330, 430, 927, 1500, 1613
MbiI CCGCTC 1 cut(s) 891
MboI GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
MfeI CAATTG 1 cut(s) 1356
MflI RGATCY 4 cut(s) 328, 428, 925, 1498
MhlI GDGCHC 4 cut(s) 161, 210, 381, 955
MluCI AATT 9 cut(s) 27, 486, 496, 607, 1221, 1356, 1506, 1620, 1666
MlyI GAGTC 4 cut(s) 437, 476, 1656, 1911
MmeI TCCRAC 5 cut(s) 143, 161, 1236, 1330, 1513
MroXI GAANNNNTTC 2 cut(s) 41, 302
MseI TTAA 8 cut(s) 318, 1151, 1173, 1227, 1440, 1503, 1550, 1623
MslI CAYNNNNRTG 4 cut(s) 384, 557, 798, 1474
MspA1I CMGCKG 2 cut(s) 728, 1765
MspI CCGG 2 cut(s) 147, 1546
MspR9I CCNGG 3 cut(s) 148, 1374, 1913
MunI CAATTG 1 cut(s) 1356
MvaI CCWGG 2 cut(s) 1374, 1913
MvnI CGCG 2 cut(s) 1081, 1817
MwoI GCNNNNNNNGC 4 cut(s) 287, 862, 1085, 1241
NciI CCSGG 1 cut(s) 148
NdeII GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
NlaIII CATG 9 cut(s) 37, 95, 265, 788, 803, 1198, 1543, 1732, 1964
NlaIV GGNNCC 4 cut(s) 330, 569, 1093, 1902
NmuCI GTSAC 3 cut(s) 191, 815, 1681
NspI RCATGY 4 cut(s) 265, 1198, 1543, 1732
NspV TTCGAA 1 cut(s) 1972
PaeI GCATGC 1 cut(s) 1543
PaeR7I CTCGAG 1 cut(s) 1749
PagI TCATGA 2 cut(s) 91, 1960
PaqCI CACCTGC 1 cut(s) 1888
PauI GCGCGC 1 cut(s) 1079
PceI AGGCCT 1 cut(s) 1846
PciI ACATGT 2 cut(s) 1194, 1728
PdmI GAANNNNTTC 2 cut(s) 41, 302
PflFI GACNNNGTC 1 cut(s) 1598
PinAI ACCGGT 1 cut(s) 1545
PkrI GCNGC 6 cut(s) 292, 730, 855, 1432, 1764, 1767
PleI GAGTC 4 cut(s) 437, 476, 1655, 1911
PpsI GAGTC 4 cut(s) 437, 476, 1655, 1911
PscI ACATGT 2 cut(s) 1194, 1728
PshAI GACNNNNGTC 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 1623
Psp124BI GAGCTC 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 1372, 1911
PspFI CCCAGC 1 cut(s) 1886
PspGI CCWGG 2 cut(s) 1372, 1911
PspN4I GGNNCC 4 cut(s) 330, 569, 1093, 1902
PspPI GGNCC 3 cut(s) 280, 1293, 1331
PstNI CAGNNNCTG 2 cut(s) 1142, 1768
PsuI RGATCY 4 cut(s) 328, 428, 925, 1498
PsyI GACNNNGTC 1 cut(s) 1598
PteI GCGCGC 1 cut(s) 1079
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 4 cut(s) 215, 365, 569, 1457
RsaNI GTAC 4 cut(s) 214, 364, 568, 1456
RseI CAYNNNNRTG 4 cut(s) 384, 557, 798, 1474
SacI GAGCTC 1 cut(s) 161
SaqAI TTAA 8 cut(s) 318, 1151, 1173, 1227, 1440, 1503, 1550, 1623
SatI GCNGC 6 cut(s) 291, 729, 854, 1431, 1763, 1766
Sau3AI GATC 6 cut(s) 164, 328, 428, 925, 1498, 1611
Sau96I GGNCC 3 cut(s) 280, 1293, 1331
SchI GAGTC 4 cut(s) 437, 476, 1656, 1911
ScrFI CCNGG 3 cut(s) 148, 1374, 1913
SduI GDGCHC 4 cut(s) 161, 210, 381, 955
SexAI ACCWGGT 1 cut(s) 1372
SfaNI GCATC 3 cut(s) 733, 1024, 1986
SfcI CTRYAG 1 cut(s) 1386
Sfr274I CTCGAG 1 cut(s) 1749
SfuI TTCGAA 1 cut(s) 1972
SinI GGWCC 1 cut(s) 1331
SlaI CTCGAG 1 cut(s) 1749
SmiMI CAYNNNNRTG 4 cut(s) 384, 557, 798, 1474
SmlI CTYRAG 4 cut(s) 209, 1073, 1493, 1749
SmoI CTYRAG 4 cut(s) 209, 1073, 1493, 1749
SphI GCATGC 1 cut(s) 1543
Sse9I AATT 9 cut(s) 27, 486, 496, 607, 1221, 1356, 1506, 1620, 1666
SseBI AGGCCT 1 cut(s) 1846
SsiI CCGC 3 cut(s) 350, 889, 1765
SspMI CTAG 4 cut(s) 426, 435, 1232, 1590
SstI GAGCTC 1 cut(s) 161
StuI AGGCCT 1 cut(s) 1846
StyD4I CCNGG 3 cut(s) 146, 1372, 1911
StyI CCWWGG 1 cut(s) 1589
TaaI ACNGT 4 cut(s) 275, 1528, 1573, 1689
TaiI ACGT 2 cut(s) 561, 1260
TaqI TCGA 6 cut(s) 155, 453, 893, 1750, 1882, 1972
TaqII GACCGA 1 cut(s) 2018
TasI AATT 9 cut(s) 27, 486, 496, 607, 1221, 1356, 1506, 1620, 1666
TatI WGTACW 2 cut(s) 213, 1455
TauI GCSGC 1 cut(s) 1768
Tru1I TTAA 8 cut(s) 318, 1151, 1173, 1227, 1440, 1503, 1550, 1623
Tru9I TTAA 8 cut(s) 318, 1151, 1173, 1227, 1440, 1503, 1550, 1623
TscAI CASTG 3 cut(s) 603, 838, 1868
TseFI GTSAC 3 cut(s) 191, 815, 1681
TseI GCWGC 5 cut(s) 290, 728, 853, 1430, 1762
Tsp45I GTSAC 3 cut(s) 191, 815, 1681
TspDTI ATGAA 8 cut(s) 30, 50, 108, 717, 816, 1581, 1820, 1995
TspGWI ACGGA 2 cut(s) 1486, 1659
TspRI CASTG 3 cut(s) 603, 838, 1868
Tth111I GACNNNGTC 1 cut(s) 1598
VneI GTGCAC 1 cut(s) 951
VpaK11BI GGWCC 1 cut(s) 1331
VspI ATTAAT 1 cut(s) 1623
XapI RAATTY 2 cut(s) 496, 607
XbaI TCTAGA 1 cut(s) 425
XceI RCATGY 4 cut(s) 265, 1198, 1543, 1732
XhoI CTCGAG 1 cut(s) 1749
XmaJI CCTAGG 1 cut(s) 1589
XmiI GTMKAC 1 cut(s) 1126
XmnI GAANNNNTTC 2 cut(s) 41, 302
XspI CTAG 4 cut(s) 426, 435, 1232, 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.