Rh5DG461300

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000175
Physical Location & Seq
Forward (+)
73272328 .. 73274627
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG461300.1

Sequence Viewer

Length: 2061 bp
ATGGATACCCTTCTGGAAGAGTTTCCAAATTCCATGAACAAATTCACATTTGACCAAAACTCCGTCCCTTCCTTTCCTGATCAAAATCCTGCTGATCAGTTTGAAGCCAATCATGAATTCACAAATCCCAGTTTCTCTCCGGCCAATTCATACCCTTATACTGATTTCTCTTCATCTTCTTCGGGTTTGAGCTCCGAGGGGGTGTATTCTCCAGACACCGGTGACTCCAATACCATCCTCAGATTCATCAGTGAGATGCTTATGGAAGAAGAAGACTTGGAGAACAAGCCTTGCATGCTTCAGGACTGTTTGGCTCTCCGAGCTGCTGAGAAGTCCTTATATGATGTCCTAGTCCAGGAGGATCCCTCTTCAACCAGTCAGAATCTCACTTCCACATACCAAAATGTTGGGAGTCCAGATGATGGCAGCAACCACAGCAGCAGTAGCTCTATTGCTGGTAGCAACTGGGTTGACTCGGATTTCAATTATATCCAAGAGGTTTTAGATACTGTGGTTCCAAACCCTTTTGTTTCAAATGGCAAGCGTGGAGTTATAGATCTAGAAGGCAACTCATCATTTTCTGCGGATGCAAGGCGACTCTCTGATGATGTGAAGTTCAGTCCGGATAAGGATGAAAGTGATGAGTCAAGAAATGGATCACGGGGCAAGAAAAATCGTTACCGGGAGGATGGTGACTATCTAGATGAAGAGAGAAGCTACAAGCAGTCGGCAGTTTACGCTGACGAAATTGAGCCAGAAGAGATGTTTGATCAGGTACTACTCTGTCAGGGTCATAAATCATCTTCTTCTCCTCAGGTATCTGAGCTCAAAGATGGAAATGGAAAGCTGCAGCGTAACGGCAAGCCAAAAGGATCCAAAGCCAAGAAAACAGGCAAGAAAAAAGCAGGTGACAATAAAGAAGTGGTGGATTTACAGACACTGCTTACTCAATGTGCACAGGCTGTTGCAAGCTATGACAAAAGGAATGCAAATGAACAACTGAAGCTGATAAGGCAGCACTCGTCTCCCTATGGCGATGGAACTCAAAGATTAGCTCATTACTTGGCCAATGGCCTCGAAGAACGCCTGACTGCGGCGGTTTCATCGTACAAGCAGTCTGTTTGCTTTTCCAGAGGTAATCTGTCGGCTGCTGTTATCTTGAAAGCTTACCGGACTTACATCACAGCATGCCCCTTTAAGAAGATGTCCAATTTTTATGCTAACCAAACGATTCGCAAGCTGGCAGAGGAGAAAACCAGGCTTCACATAATTGATTTTGGTATACTCTATGGGTACCAATGGCCTTGCCTTATCCAAGCTCTCTCAAAAAGACCTGGCGGACCTCCCATGGTTCGCATTACTGGAATCGAGTTTCCCCAATCAGGTTTTCGACCTTCAGAAGGGCTTGAAGAGACAGGGCGCCGCCTACAGAATTACTGCAAGAGAGTCAATGTCCCATTTGAGTACACTGCCATAGCAAAGAATTGGGAAACCATTCAGTATGAGGATATCAAAGTCGAGAGAGATGAGGTGACCGTAGTGAACTGCTTGTACAGGTTAAAGAACTTACCAGATGACGTAGTGTTGGACAGCCCCAGAGACACAGTTTTGAAGCTGATCAGGAGAATCAACCCAGATGTATTCATCCATGGAATTGTTAATGGAACCTACAACGCACCCTTCTTTGACACACGGTTCCGGGAGACACTCTACCATTTCTCTTCCTTGTTTGATATGTATGAGGAGACTTTGCCCAGAGAAGATCCAGACAGGCTTCTCTTTGAACAAGAGGTATTTGGTAGAGATCTTATCAATGTGGTAGCATGTGAAGGTTCAATGAGGCTTGAAAGACCTGAAACATACAAGCAGTGGCAGATGAGAAATATAAGAGCTGGGTTCAAGCAGTTGCCATTAGATCAGGACCTCTTGAAGAGAATAAAGACTATGGTGAAGTCAGAATATCATAAAGATTTTGTTGTGGATGAAGATGGCAAGTGGGTCTTGCACGGATGGAAAGGCCGAATAATCCATGCTATTTCTTGCTGGAAATCTGCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

686

Amino Acids

78.01

Weight (kDa)

5.4

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 312 - 683 1.5e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 896
Acc36I ACCTGC 1 cut(s) 896
Acc65I GGTACC 1 cut(s) 1293
AccB1I GGYRCC 2 cut(s) 1293, 1419
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 1 cut(s) 1282
AccIII TCCGGA 1 cut(s) 622
AciI CCGC 5 cut(s) 584, 1094, 1097, 1338, 1423
AclWI GGATC 6 cut(s) 356, 369, 664, 867, 880, 1757
AcoI YGGCCR 2 cut(s) 141, 1065
AcsI RAATTY 3 cut(s) 28, 41, 116
AcuI CTGAAG 3 cut(s) 284, 1022, 1380
AcyI GRCGYC 1 cut(s) 1420
AfaI GTAC 5 cut(s) 777, 1109, 1295, 1466, 1553
AfiI CCNNNNNNNGG 6 cut(s) 218, 355, 422, 1093, 1382, 1400
AgeI ACCGGT 1 cut(s) 218
AjnI CCWGG 3 cut(s) 354, 1256, 1333
AjuI GAANNNNNNNTTGG 4 cut(s) 101, 133, 1371, 1403
Alw21I GWGCWC 3 cut(s) 194, 828, 958
Alw26I GTCTC 5 cut(s) 1029, 1406, 1593, 1697, 1739
Alw44I GTGCAC 1 cut(s) 954
AlwI GGATC 6 cut(s) 356, 369, 664, 867, 880, 1757
AlwNI CAGNNNCTG 1 cut(s) 940
Aor13HI TCCGGA 1 cut(s) 622
AoxI GGCC 5 cut(s) 141, 1065, 1072, 1301, 2017
ApaLI GTGCAC 1 cut(s) 954
ApeKI GCWGC 7 cut(s) 323, 426, 438, 847, 850, 1015, 1148
ApoI RAATTY 3 cut(s) 28, 41, 116
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 3 cut(s) 21, 41, 1494
Asp718I GGTACC 1 cut(s) 1293
AspLEI GCGC 1 cut(s) 1422
AspS9I GGNCC 2 cut(s) 1340, 1921
AsuC2I CCSGG 2 cut(s) 683, 1700
AsuHPI GGTGA 5 cut(s) 233, 704, 920, 1543, 1960
AvaII GGWCC 2 cut(s) 1340, 1921
AxyI CCTNAGG 1 cut(s) 813
BaeGI GKGCMC 1 cut(s) 958
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BalI TGGCCA 1 cut(s) 1067
BamHI GGATCC 2 cut(s) 361, 872
BanI GGYRCC 2 cut(s) 1293, 1419
BanII GRGCYC 2 cut(s) 194, 828
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 3 cut(s) 194, 828, 958
BbvI GCAGC 7 cut(s) 310, 438, 450, 834, 862, 1027, 1135
BccI CCATC 7 cut(s) 242, 416, 683, 827, 1031, 1982, 2004
BceAI ACGGC 1 cut(s) 874
BciT130I CCWGG 3 cut(s) 356, 1258, 1335
BclI TGATCA 4 cut(s) 79, 94, 769, 1617
BcnI CCSGG 2 cut(s) 683, 1700
BcoDI GTCTC 5 cut(s) 1029, 1406, 1593, 1697, 1739
BfaI CTAG 3 cut(s) 350, 560, 701
BfmI CTRYAG 2 cut(s) 848, 1427
BfoI RGCGCY 1 cut(s) 1423
BfuAI ACCTGC 1 cut(s) 896
BglII AGATCT 2 cut(s) 556, 1804
BisI GCNGC 9 cut(s) 324, 427, 439, 848, 851, 1016, 1095, 1149, 1423
BlsI GCNGC 9 cut(s) 325, 428, 440, 849, 852, 1017, 1096, 1150, 1424
Bme1390I CCNGG 5 cut(s) 356, 683, 1258, 1335, 1700
Bme18I GGWCC 2 cut(s) 1340, 1921
BmgT120I GGNCC 2 cut(s) 1340, 1921
BmiI GGNNCC 7 cut(s) 363, 516, 874, 1295, 1421, 1666, 1697
BmrFI CCNGG 5 cut(s) 356, 683, 1258, 1335, 1700
BmrI ACTGGG 2 cut(s) 123, 475
BmsI GCATC 2 cut(s) 246, 577
BmuI ACTGGG 2 cut(s) 123, 475
BpiI GAAGAC 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BpmI CTGGAG 1 cut(s) 195
BpuMI CCSGG 2 cut(s) 683, 1700
BsaBI GATNNNNATC 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 1420
BsaJI CCNNGG 3 cut(s) 195, 1347, 1648
BsaWI WCCGGW 3 cut(s) 218, 622, 1170
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 6 cut(s) 218, 355, 422, 1093, 1382, 1400
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 4 cut(s) 129, 375, 470, 1366
Bse21I CCTNAGG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 84
BseAI TCCGGA 1 cut(s) 622
BseBI CCWGG 3 cut(s) 356, 1258, 1335
BseDI CCNNGG 3 cut(s) 195, 1347, 1648
BseGI GGATG 7 cut(s) 234, 592, 637, 694, 1644, 1987, 2015
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 6 cut(s) 218, 355, 422, 1093, 1382, 1400
BseMII CTCAG 4 cut(s) 253, 318, 813, 827
BseNI ACTGG 4 cut(s) 129, 375, 470, 1366
BseRI GAGGAG 3 cut(s) 801, 1262, 1757
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 7 cut(s) 310, 438, 450, 834, 862, 1027, 1135
BseYI CCCAGC 1 cut(s) 1892
BshFI GGCC 5 cut(s) 143, 1067, 1074, 1303, 2019
BshNI GGYRCC 2 cut(s) 1293, 1419
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 3 cut(s) 194, 828, 958
BsiSI CCGG 6 cut(s) 140, 219, 623, 682, 1171, 1699
BslFI GGGAC 2 cut(s) 50, 1439
BslI CCNNNNNNNGG 6 cut(s) 218, 355, 422, 1093, 1382, 1400
BsmAI GTCTC 5 cut(s) 1029, 1406, 1593, 1697, 1739
BsmBI CGTCTC 1 cut(s) 1029
BsmFI GGGAC 2 cut(s) 50, 1439
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 5 cut(s) 143, 1067, 1074, 1303, 2019
Bsp1286I GDGCHC 3 cut(s) 194, 828, 958
Bsp13I TCCGGA 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 1551
Bsp19I CCATGG 2 cut(s) 1347, 1648
BspACI CCGC 5 cut(s) 584, 1094, 1097, 1338, 1423
BspANI GGCC 5 cut(s) 143, 1067, 1074, 1303, 2019
BspCNI CTCAG 4 cut(s) 252, 319, 814, 826
BspEI TCCGGA 1 cut(s) 622
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 7 cut(s) 363, 516, 874, 1295, 1421, 1666, 1697
BspMAI CTGCAG 1 cut(s) 852
BspMI ACCTGC 1 cut(s) 896
BspPI GGATC 6 cut(s) 356, 369, 664, 867, 880, 1757
BspT107I GGYRCC 2 cut(s) 1293, 1419
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 1551
BsrI ACTGG 4 cut(s) 129, 375, 470, 1366
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 3 cut(s) 195, 1347, 1648
BssNAI GTATAC 1 cut(s) 1283
BssNI GRCGYC 1 cut(s) 1420
BssT1I CCWWGG 2 cut(s) 1347, 1648
Bst1107I GTATAC 1 cut(s) 1283
Bst2UI CCWGG 3 cut(s) 356, 1258, 1335
Bst4CI ACNGT 5 cut(s) 308, 511, 1537, 1606, 1695
Bst6I CTCTTC 8 cut(s) 12, 175, 373, 702, 753, 1404, 1726, 1925
BstACI GRCGYC 1 cut(s) 1420
BstAUI TGTACA 1 cut(s) 1551
BstC8I GCNNGC 7 cut(s) 296, 542, 863, 970, 1189, 1238, 1242
BstDEI CTNAG 4 cut(s) 239, 327, 813, 822
BstDSI CCRYGG 2 cut(s) 1347, 1648
BstEII GGTNACC 1 cut(s) 1531
BstENI CCTNNNNNAGG 2 cut(s) 353, 1398
BstF5I GGATG 7 cut(s) 234, 592, 637, 694, 1644, 1987, 2015
BstH2I RGCGCY 1 cut(s) 1423
BstHHI GCGC 1 cut(s) 1422
BstMAI GTCTC 5 cut(s) 1029, 1406, 1593, 1697, 1739
BstMWI GCNNNNNNNGC 6 cut(s) 295, 320, 435, 444, 737, 1012
BstNI CCWGG 3 cut(s) 356, 1258, 1335
BstNSI RCATGY 3 cut(s) 298, 1191, 1827
BstPI GGTNACC 1 cut(s) 1531
BstSCI CCNGG 5 cut(s) 354, 681, 1256, 1333, 1698
BstSFI CTRYAG 2 cut(s) 848, 1427
BstSLI GKGCMC 1 cut(s) 958
BstV1I GCAGC 7 cut(s) 310, 438, 450, 834, 862, 1027, 1135
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 5 cut(s) 361, 556, 872, 1762, 1804
BstXI CCANNNNNNTGG 1 cut(s) 407
BstYI RGATCY 5 cut(s) 361, 556, 872, 1762, 1804
BstZ17I GTATAC 1 cut(s) 1283
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 5 cut(s) 143, 1067, 1074, 1303, 2019
BtgI CCRYGG 2 cut(s) 1347, 1648
BtgZI GCGATG 1 cut(s) 1050
BtsCI GGATG 7 cut(s) 234, 592, 637, 694, 1644, 1987, 2015
BtsI GCAGTG 3 cut(s) 938, 1467, 1874
BtsIMutI CAGTG 4 cut(s) 256, 938, 1467, 1874
BveI ACCTGC 1 cut(s) 896
Cac8I GCNNGC 7 cut(s) 296, 542, 863, 970, 1189, 1238, 1242
CaiI CAGNNNCTG 1 cut(s) 940
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 1422
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 1340, 1921
Csp6I GTAC 5 cut(s) 776, 1108, 1294, 1465, 1552
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 8 cut(s) 34, 113, 295, 1188, 1348, 1649, 1824, 2030
CviQI GTAC 5 cut(s) 776, 1108, 1294, 1465, 1552
DdeI CTNAG 4 cut(s) 239, 327, 813, 822
DinI GGCGCC 1 cut(s) 1421
EaeI YGGCCR 2 cut(s) 141, 1065
Eam1104I CTCTTC 8 cut(s) 12, 175, 373, 702, 753, 1404, 1726, 1925
EarI CTCTTC 8 cut(s) 12, 175, 373, 702, 753, 1404, 1726, 1925
EciI GGCGGA 1 cut(s) 1353
Ecl136II GAGCTC 2 cut(s) 192, 826
Eco130I CCWWGG 2 cut(s) 1347, 1648
Eco24I GRGCYC 2 cut(s) 194, 828
Eco32I GATATC 1 cut(s) 1510
Eco47I GGWCC 2 cut(s) 1340, 1921
Eco53kI GAGCTC 2 cut(s) 192, 826
Eco57I CTGAAG 3 cut(s) 284, 1022, 1380
Eco81I CCTNAGG 1 cut(s) 813
Eco91I GGTNACC 1 cut(s) 1531
EcoICRI GAGCTC 2 cut(s) 192, 826
EcoNI CCTNNNNNAGG 2 cut(s) 353, 1398
EcoO109I RGGNCCY 1 cut(s) 1921
EcoO65I GGTNACC 1 cut(s) 1531
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 3 cut(s) 354, 1256, 1333
EcoRV GATATC 1 cut(s) 1510
EcoT14I CCWWGG 2 cut(s) 1347, 1648
EcoT38I GRGCYC 2 cut(s) 194, 828
EgeI GGCGCC 1 cut(s) 1421
EheI GGCGCC 1 cut(s) 1421
ErhI CCWWGG 2 cut(s) 1347, 1648
Esp3I CGTCTC 1 cut(s) 1029
FaeI CATG 8 cut(s) 37, 116, 298, 1191, 1351, 1652, 1827, 2033
FalI AAGNNNNNCTT 2 cut(s) 1985, 2017
FaqI GGGAC 2 cut(s) 50, 1439
FatI CATG 8 cut(s) 33, 112, 294, 1187, 1347, 1648, 1823, 2029
FbaI TGATCA 4 cut(s) 79, 94, 769, 1617
FblI GTMKAC 1 cut(s) 1282
Fnu4HI GCNGC 9 cut(s) 324, 427, 439, 848, 851, 1016, 1095, 1149, 1423
FokI GGATG 7 cut(s) 221, 599, 644, 701, 1631, 1994, 2022
FriOI GRGCYC 2 cut(s) 194, 828
Fsp4HI GCNGC 9 cut(s) 324, 427, 439, 848, 851, 1016, 1095, 1149, 1423
FspBI CTAG 3 cut(s) 350, 560, 701
GlaI GCGC 1 cut(s) 1421
GluI GCNGC 9 cut(s) 324, 427, 439, 848, 851, 1016, 1095, 1149, 1423
GsaI CCCAGC 1 cut(s) 1896
GsuI CTGGAG 1 cut(s) 195
HaeII RGCGCY 1 cut(s) 1423
HaeIII GGCC 5 cut(s) 143, 1067, 1074, 1303, 2019
HapII CCGG 6 cut(s) 140, 219, 623, 682, 1171, 1699
HhaI GCGC 1 cut(s) 1422
Hin1I GRCGYC 1 cut(s) 1420
Hin1II CATG 8 cut(s) 37, 116, 298, 1191, 1351, 1652, 1827, 2033
Hin6I GCGC 1 cut(s) 1420
HinP1I GCGC 1 cut(s) 1420
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 1164
HpaII CCGG 6 cut(s) 140, 219, 623, 682, 1171, 1699
HphI GGTGA 5 cut(s) 233, 704, 920, 1543, 1960
Hpy166II GTNNAC 6 cut(s) 472, 736, 956, 1283, 1467, 1543
Hpy188I TCNGA 9 cut(s) 196, 242, 320, 381, 478, 604, 823, 1399, 1957
Hpy8I GTNNAC 6 cut(s) 472, 736, 956, 1283, 1467, 1543
HpyAV CCTTC 7 cut(s) 20, 78, 557, 1394, 1404, 1690, 1823
HpyCH4III ACNGT 5 cut(s) 308, 511, 1537, 1606, 1695
HpyCH4IV ACGT 1 cut(s) 1578
HpyCH4V TGCA 8 cut(s) 294, 590, 850, 956, 968, 989, 1440, 2005
HpyF10VI GCNNNNNNNGC 6 cut(s) 295, 320, 435, 444, 737, 1012
HpyF3I CTNAG 4 cut(s) 239, 327, 813, 822
HpySE526I ACGT 1 cut(s) 1578
Hsp92I GRCGYC 1 cut(s) 1420
Hsp92II CATG 8 cut(s) 37, 116, 298, 1191, 1351, 1652, 1827, 2033
HspAI GCGC 1 cut(s) 1420
KasI GGCGCC 1 cut(s) 1419
Kpn2I TCCGGA 1 cut(s) 622
KpnI GGTACC 1 cut(s) 1297
Ksp22I TGATCA 4 cut(s) 79, 94, 769, 1617
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 7 cut(s) 310, 438, 450, 834, 862, 1027, 1135
LweI GCATC 2 cut(s) 246, 577
MaeI CTAG 3 cut(s) 350, 560, 701
MaeII ACGT 1 cut(s) 1578
MaeIII GTNAC 6 cut(s) 221, 677, 692, 854, 908, 1531
MflI RGATCY 5 cut(s) 361, 556, 872, 1762, 1804
MhlI GDGCHC 3 cut(s) 194, 828, 958
MlsI TGGCCA 1 cut(s) 1067
MluNI TGGCCA 1 cut(s) 1067
Mly113I GGCGCC 1 cut(s) 1420
MlyI GAGTC 6 cut(s) 218, 421, 467, 591, 653, 1455
MmeI TCCRAC 1 cut(s) 1566
Mox20I TGGCCA 1 cut(s) 1067
MroI TCCGGA 1 cut(s) 622
MroXI GAANNNNTTC 3 cut(s) 21, 41, 1494
MscI TGGCCA 1 cut(s) 1067
MseI TTAA 3 cut(s) 1197, 1559, 1659
Msp20I TGGCCA 1 cut(s) 1067
MspI CCGG 6 cut(s) 140, 219, 623, 682, 1171, 1699
MspR9I CCNGG 5 cut(s) 356, 683, 1258, 1335, 1700
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 3 cut(s) 356, 1258, 1335
MwoI GCNNNNNNNGC 6 cut(s) 295, 320, 435, 444, 737, 1012
NarI GGCGCC 1 cut(s) 1420
NciI CCSGG 2 cut(s) 683, 1700
NcoI CCATGG 2 cut(s) 1347, 1648
NlaIII CATG 8 cut(s) 37, 116, 298, 1191, 1351, 1652, 1827, 2033
NlaIV GGNNCC 7 cut(s) 363, 516, 874, 1295, 1421, 1666, 1697
NmuCI GTSAC 4 cut(s) 221, 692, 908, 1531
NspI RCATGY 3 cut(s) 298, 1191, 1827
PaeI GCATGC 2 cut(s) 298, 1191
PagI TCATGA 1 cut(s) 112
PaqCI CACCTGC 1 cut(s) 896
PctI GAATGC 1 cut(s) 991
PdmI GAANNNNTTC 3 cut(s) 21, 41, 1494
PfeI GAWTC 5 cut(s) 243, 382, 1231, 1365, 1626
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 2 cut(s) 354, 1698
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 9 cut(s) 325, 428, 440, 849, 852, 1017, 1096, 1150, 1424
PleI GAGTC 6 cut(s) 218, 420, 467, 591, 652, 1454
PluTI GGCGCC 1 cut(s) 1423
PpsI GAGTC 6 cut(s) 218, 420, 467, 591, 652, 1454
PpuMI RGGWCCY 1 cut(s) 1921
Psp124BI GAGCTC 2 cut(s) 194, 828
Psp5II RGGWCCY 1 cut(s) 1921
Psp6I CCWGG 3 cut(s) 354, 1256, 1333
PspEI GGTNACC 1 cut(s) 1531
PspFI CCCAGC 1 cut(s) 1892
PspGI CCWGG 3 cut(s) 354, 1256, 1333
PspN4I GGNNCC 7 cut(s) 363, 516, 874, 1295, 1421, 1666, 1697
PspPI GGNCC 2 cut(s) 1340, 1921
PspPPI RGGWCCY 1 cut(s) 1921
PsrI GAACNNNNNNTAC 2 cut(s) 1535, 1567
PstI CTGCAG 1 cut(s) 852
PstNI CAGNNNCTG 1 cut(s) 940
PsuI RGATCY 5 cut(s) 361, 556, 872, 1762, 1804
RsaI GTAC 5 cut(s) 777, 1109, 1295, 1466, 1553
RsaNI GTAC 5 cut(s) 776, 1108, 1294, 1465, 1552
SacI GAGCTC 2 cut(s) 194, 828
SaqAI TTAA 3 cut(s) 1197, 1559, 1659
SatI GCNGC 9 cut(s) 324, 427, 439, 848, 851, 1016, 1095, 1149, 1423
Sau96I GGNCC 2 cut(s) 1340, 1921
SchI GAGTC 6 cut(s) 218, 421, 467, 591, 653, 1455
ScrFI CCNGG 5 cut(s) 356, 683, 1258, 1335, 1700
SduI GDGCHC 3 cut(s) 194, 828, 958
SfaNI GCATC 2 cut(s) 246, 577
SfcI CTRYAG 2 cut(s) 848, 1427
SfoI GGCGCC 1 cut(s) 1421
SgrAI CRCCGGYG 1 cut(s) 218
SinI GGWCC 2 cut(s) 1340, 1921
SphI GCATGC 2 cut(s) 298, 1191
SsiI CCGC 5 cut(s) 584, 1094, 1097, 1338, 1423
SspDI GGCGCC 1 cut(s) 1419
SspMI CTAG 3 cut(s) 350, 560, 701
SstI GAGCTC 2 cut(s) 194, 828
StyD4I CCNGG 5 cut(s) 354, 681, 1256, 1333, 1698
StyI CCWWGG 2 cut(s) 1347, 1648
TaaI ACNGT 5 cut(s) 308, 511, 1537, 1606, 1695
TaiI ACGT 1 cut(s) 1581
TaqI TCGA 4 cut(s) 1077, 1368, 1390, 1518
TatI WGTACW 2 cut(s) 1464, 1551
TauI GCSGC 2 cut(s) 1097, 1425
TfiI GAWTC 5 cut(s) 243, 382, 1231, 1365, 1626
Tru1I TTAA 3 cut(s) 1197, 1559, 1659
Tru9I TTAA 3 cut(s) 1197, 1559, 1659
TscAI CASTG 4 cut(s) 256, 945, 1474, 1874
TseFI GTSAC 4 cut(s) 221, 692, 908, 1531
TseI GCWGC 7 cut(s) 323, 426, 438, 847, 850, 1015, 1148
Tsp45I GTSAC 4 cut(s) 221, 692, 908, 1531
TspGWI ACGGA 2 cut(s) 52, 2022
TspRI CASTG 4 cut(s) 256, 945, 1474, 1874
Van91I CCANNNNNTGG 1 cut(s) 422
VneI GTGCAC 1 cut(s) 954
VpaK11BI GGWCC 2 cut(s) 1340, 1921
XagI CCTNNNNNAGG 2 cut(s) 353, 1398
XapI RAATTY 3 cut(s) 28, 41, 116
XbaI TCTAGA 2 cut(s) 559, 700
XceI RCATGY 3 cut(s) 298, 1191, 1827
XmiI GTMKAC 1 cut(s) 1282
XmnI GAANNNNTTC 3 cut(s) 21, 41, 1494
XspI CTAG 3 cut(s) 350, 560, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.