Belongs to the GRAS family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1711 |
| Acc36I | ACCTGC | 1 cut(s) | 1711 |
| Acc65I | GGTACC | 1 cut(s) | 600 |
| AccB1I | GGYRCC | 2 cut(s) | 600, 1440 |
| AccI | GTMKAC | 1 cut(s) | 954 |
| AccII | CGCG | 1 cut(s) | 1782 |
| AciI | CCGC | 1 cut(s) | 1444 |
| AclWI | GGATC | 4 cut(s) | 694, 776, 913, 1122 |
| AcoI | YGGCCR | 1 cut(s) | 19 |
| AcsI | RAATTY | 6 cut(s) | 31, 553, 640, 1175, 1587, 1636 |
| AcuI | CTGAAG | 1 cut(s) | 129 |
| AcyI | GRCGYC | 1 cut(s) | 1441 |
| AfaI | GTAC | 6 cut(s) | 169, 197, 347, 602, 635, 1487 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1403 |
| AflIII | ACRYGT | 1 cut(s) | 917 |
| AgeI | ACCGGT | 2 cut(s) | 1575, 1794 |
| AjnI | CCWGG | 2 cut(s) | 225, 1402 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1392, 1424 |
| Alw21I | GWGCWC | 3 cut(s) | 192, 985, 1950 |
| Alw26I | GTCTC | 7 cut(s) | 43, 501, 1346, 1427, 1592, 1617, 1874 |
| Alw44I | GTGCAC | 1 cut(s) | 981 |
| AlwI | GGATC | 4 cut(s) | 694, 776, 913, 1122 |
| AoxI | GGCC | 6 cut(s) | 19, 262, 1099, 1323, 1767, 1874 |
| ApaLI | GTGCAC | 1 cut(s) | 981 |
| ApeKI | GCWGC | 7 cut(s) | 272, 431, 444, 761, 883, 1927, 2029 |
| ApoI | RAATTY | 6 cut(s) | 31, 553, 640, 1175, 1587, 1636 |
| AseI | ATTAAT | 1 cut(s) | 1653 |
| AsiGI | ACCGGT | 2 cut(s) | 1575, 1794 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1515 |
| Asp718I | GGTACC | 1 cut(s) | 600 |
| AspLEI | GCGC | 2 cut(s) | 1443, 1750 |
| AspS9I | GGNCC | 3 cut(s) | 262, 1323, 1361 |
| AsuC2I | CCSGG | 1 cut(s) | 1943 |
| AsuHPI | GGTGA | 2 cut(s) | 164, 2030 |
| AsuII | TTCGAA | 1 cut(s) | 1806 |
| AvaII | GGWCC | 1 cut(s) | 1361 |
| BaeGI | GKGCMC | 1 cut(s) | 985 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 592, 625 |
| BanI | GGYRCC | 2 cut(s) | 600, 1440 |
| BanII | GRGCYC | 2 cut(s) | 69, 1950 |
| BbsI | GAAGAC | 2 cut(s) | 228, 1970 |
| Bbv12I | GWGCWC | 3 cut(s) | 192, 985, 1950 |
| BbvCI | CCTCAGC | 1 cut(s) | 864 |
| BbvI | GCAGC | 7 cut(s) | 259, 418, 456, 748, 895, 1939, 2016 |
| BccI | CCATC | 4 cut(s) | 336, 1975, 2009, 2031 |
| BceAI | ACGGC | 1 cut(s) | 436 |
| BciT130I | CCWGG | 2 cut(s) | 227, 1404 |
| BclI | TGATCA | 1 cut(s) | 46 |
| BcnI | CCSGG | 1 cut(s) | 1943 |
| BcoDI | GTCTC | 7 cut(s) | 43, 501, 1346, 1427, 1592, 1617, 1874 |
| BfaI | CTAG | 5 cut(s) | 215, 558, 873, 1103, 1902 |
| BfmI | CTRYAG | 1 cut(s) | 2030 |
| BfoI | RGCGCY | 1 cut(s) | 1444 |
| BfuAI | ACCTGC | 1 cut(s) | 1711 |
| BisI | GCNGC | 8 cut(s) | 273, 432, 445, 762, 884, 1444, 1928, 2030 |
| BlpI | GCTNAGC | 1 cut(s) | 1447 |
| BlsI | GCNGC | 8 cut(s) | 274, 433, 446, 763, 885, 1445, 1929, 2031 |
| Bme1390I | CCNGG | 3 cut(s) | 227, 1404, 1943 |
| Bme18I | GGWCC | 1 cut(s) | 1361 |
| BmgT120I | GGNCC | 3 cut(s) | 262, 1323, 1361 |
| BmiI | GGNNCC | 3 cut(s) | 68, 602, 1442 |
| BmrFI | CCNGG | 3 cut(s) | 227, 1404, 1943 |
| BmsI | GCATC | 3 cut(s) | 283, 656, 2016 |
| BpiI | GAAGAC | 2 cut(s) | 228, 1970 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1066, 1098 |
| BpmI | CTGGAG | 2 cut(s) | 248, 1544 |
| Bpu10I | CCTNAGC | 1 cut(s) | 864 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1447 |
| Bpu14I | TTCGAA | 1 cut(s) | 1806 |
| BpuEI | CTTGAG | 3 cut(s) | 176, 614, 1013 |
| BpuMI | CCSGG | 1 cut(s) | 1943 |
| BsaBI | GATNNNNATC | 2 cut(s) | 780, 1132 |
| BsaHI | GRCGYC | 1 cut(s) | 1441 |
| BsaJI | CCNNGG | 1 cut(s) | 22 |
| BsaWI | WCCGGW | 2 cut(s) | 1575, 1794 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1403 |
| Bse118I | RCCGGY | 3 cut(s) | 942, 1575, 1794 |
| Bse1I | ACTGG | 4 cut(s) | 604, 1360, 1527, 1863 |
| Bse3DI | GCAATG | 2 cut(s) | 548, 1494 |
| Bse8I | GATNNNNATC | 2 cut(s) | 780, 1132 |
| BseBI | CCWGG | 2 cut(s) | 227, 1404 |
| BseDI | CCNNGG | 1 cut(s) | 22 |
| BseGI | GGATG | 4 cut(s) | 328, 647, 2031, 2042 |
| BseJI | GATNNNNATC | 2 cut(s) | 780, 1132 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1403 |
| BseMI | GCAATG | 2 cut(s) | 548, 1494 |
| BseMII | CTCAG | 5 cut(s) | 378, 822, 855, 1359, 1869 |
| BseNI | ACTGG | 4 cut(s) | 604, 1360, 1527, 1863 |
| BseSI | GKGCMC | 1 cut(s) | 985 |
| BseXI | GCAGC | 7 cut(s) | 259, 418, 456, 748, 895, 1939, 2016 |
| BseYI | CCCAGC | 1 cut(s) | 1916 |
| Bsh1236I | CGCG | 1 cut(s) | 1782 |
| BshFI | GGCC | 6 cut(s) | 21, 264, 1101, 1325, 1769, 1876 |
| BshNI | GGYRCC | 2 cut(s) | 600, 1440 |
| BshTI | ACCGGT | 2 cut(s) | 1575, 1794 |
| BsiHKAI | GWGCWC | 3 cut(s) | 192, 985, 1950 |
| BsiSI | CCGG | 4 cut(s) | 943, 1576, 1795, 1942 |
| BslFI | GGGAC | 3 cut(s) | 1702, 1760, 1869 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1403 |
| BsmAI | GTCTC | 7 cut(s) | 43, 501, 1346, 1427, 1592, 1617, 1874 |
| BsmBI | CGTCTC | 1 cut(s) | 1617 |
| BsmFI | GGGAC | 3 cut(s) | 1702, 1760, 1869 |
| BsmI | GAATGC | 1 cut(s) | 1045 |
| BsnI | GGCC | 6 cut(s) | 21, 264, 1101, 1325, 1769, 1876 |
| Bsp119I | TTCGAA | 1 cut(s) | 1806 |
| Bsp1286I | GDGCHC | 4 cut(s) | 69, 192, 985, 1950 |
| Bsp1407I | TGTACA | 1 cut(s) | 633 |
| Bsp143I | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1447 |
| Bsp19I | CCATGG | 1 cut(s) | 22 |
| Bsp68I | TCGCGA | 1 cut(s) | 1782 |
| BspACI | CCGC | 1 cut(s) | 1444 |
| BspANI | GGCC | 6 cut(s) | 21, 264, 1101, 1325, 1769, 1876 |
| BspCNI | CTCAG | 5 cut(s) | 377, 823, 856, 1358, 1870 |
| BspFNI | CGCG | 1 cut(s) | 1782 |
| BspHI | TCATGA | 2 cut(s) | 79, 844 |
| BspLI | GGNNCC | 3 cut(s) | 68, 602, 1442 |
| BspMAI | CTGCAG | 1 cut(s) | 2034 |
| BspMI | ACCTGC | 1 cut(s) | 1711 |
| BspPI | GGATC | 4 cut(s) | 694, 776, 913, 1122 |
| BspQI | GCTCTTC | 2 cut(s) | 1739, 1757 |
| BspT104I | TTCGAA | 1 cut(s) | 1806 |
| BspT107I | GGYRCC | 2 cut(s) | 600, 1440 |
| BsrDI | GCAATG | 2 cut(s) | 548, 1494 |
| BsrFI | RCCGGY | 3 cut(s) | 942, 1575, 1794 |
| BsrGI | TGTACA | 1 cut(s) | 633 |
| BsrI | ACTGG | 4 cut(s) | 604, 1360, 1527, 1863 |
| BssAI | RCCGGY | 3 cut(s) | 942, 1575, 1794 |
| BssECI | CCNNGG | 1 cut(s) | 22 |
| BssMI | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| BssNI | GRCGYC | 1 cut(s) | 1441 |
| BssT1I | CCWWGG | 1 cut(s) | 22 |
| Bst2UI | CCWGG | 2 cut(s) | 227, 1404 |
| Bst6I | CTCTTC | 5 cut(s) | 46, 1152, 1425, 1739, 1757 |
| BstACI | GRCGYC | 1 cut(s) | 1441 |
| BstAUI | TGTACA | 1 cut(s) | 633 |
| BstBI | TTCGAA | 1 cut(s) | 1806 |
| BstC8I | GCNNGC | 5 cut(s) | 1111, 1210, 1327, 1899, 2079 |
| BstDSI | CCRYGG | 1 cut(s) | 22 |
| BstF5I | GGATG | 4 cut(s) | 328, 647, 2031, 2042 |
| BstFNI | CGCG | 1 cut(s) | 1782 |
| BstH2I | RGCGCY | 1 cut(s) | 1444 |
| BstHHI | GCGC | 2 cut(s) | 1443, 1750 |
| BstKTI | GATC | 6 cut(s) | 49, 689, 784, 908, 1130, 1557 |
| BstMAI | GTCTC | 7 cut(s) | 43, 501, 1346, 1427, 1592, 1617, 1874 |
| BstMBI | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 390, 420, 767, 892, 1115, 2066 |
| BstNI | CCWGG | 2 cut(s) | 227, 1404 |
| BstNSI | RCATGY | 4 cut(s) | 247, 541, 921, 1212 |
| BstSCI | CCNGG | 3 cut(s) | 225, 1402, 1941 |
| BstSFI | CTRYAG | 1 cut(s) | 2030 |
| BstSLI | GKGCMC | 1 cut(s) | 985 |
| BstUI | CGCG | 1 cut(s) | 1782 |
| BstV1I | GCAGC | 7 cut(s) | 259, 418, 456, 748, 895, 1939, 2016 |
| BstV2I | GAAGAC | 2 cut(s) | 228, 1970 |
| BstX2I | RGATCY | 2 cut(s) | 905, 1127 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1522 |
| BstYI | RGATCY | 2 cut(s) | 905, 1127 |
| BsuRI | GGCC | 6 cut(s) | 21, 264, 1101, 1325, 1769, 1876 |
| BtgI | CCRYGG | 1 cut(s) | 22 |
| BtsCI | GGATG | 4 cut(s) | 328, 647, 2031, 2042 |
| BtsI | GCAGTG | 1 cut(s) | 1898 |
| BtsIMutI | CAGTG | 4 cut(s) | 611, 629, 1770, 1898 |
| BtuMI | TCGCGA | 1 cut(s) | 1782 |
| BveI | ACCTGC | 1 cut(s) | 1711 |
| Cac8I | GCNNGC | 5 cut(s) | 1111, 1210, 1327, 1899, 2079 |
| CciI | TCATGA | 2 cut(s) | 79, 844 |
| CfoI | GCGC | 2 cut(s) | 1443, 1750 |
| Cfr10I | RCCGGY | 3 cut(s) | 942, 1575, 1794 |
| Cfr13I | GGNCC | 3 cut(s) | 262, 1323, 1361 |
| CseI | GACGC | 1 cut(s) | 1634 |
| CsiI | ACCWGGT | 1 cut(s) | 1402 |
| Csp6I | GTAC | 6 cut(s) | 168, 196, 346, 601, 634, 1486 |
| CspAI | ACCGGT | 2 cut(s) | 1575, 1794 |
| CviQI | GTAC | 6 cut(s) | 168, 196, 346, 601, 634, 1486 |
| DinI | GGCGCC | 1 cut(s) | 1442 |
| DpnI | GATC | 6 cut(s) | 48, 688, 783, 907, 1129, 1556 |
| DpnII | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| DraI | TTTAAA | 1 cut(s) | 858 |
| EaeI | YGGCCR | 1 cut(s) | 19 |
| Eam1104I | CTCTTC | 5 cut(s) | 46, 1152, 1425, 1739, 1757 |
| EarI | CTCTTC | 5 cut(s) | 46, 1152, 1425, 1739, 1757 |
| Ecl136II | GAGCTC | 1 cut(s) | 1948 |
| Eco130I | CCWWGG | 1 cut(s) | 22 |
| Eco147I | AGGCCT | 2 cut(s) | 1101, 1876 |
| Eco24I | GRGCYC | 2 cut(s) | 69, 1950 |
| Eco32I | GATATC | 1 cut(s) | 508 |
| Eco47I | GGWCC | 1 cut(s) | 1361 |
| Eco53kI | GAGCTC | 1 cut(s) | 1948 |
| Eco57I | CTGAAG | 1 cut(s) | 129 |
| EcoICRI | GAGCTC | 1 cut(s) | 1948 |
| EcoRI | GAATTC | 2 cut(s) | 553, 1175 |
| EcoRII | CCWGG | 2 cut(s) | 225, 1402 |
| EcoRV | GATATC | 1 cut(s) | 508 |
| EcoT14I | CCWWGG | 1 cut(s) | 22 |
| EcoT22I | ATGCAT | 1 cut(s) | 1674 |
| EcoT38I | GRGCYC | 2 cut(s) | 69, 1950 |
| EgeI | GGCGCC | 1 cut(s) | 1442 |
| EheI | GGCGCC | 1 cut(s) | 1442 |
| ErhI | CCWWGG | 1 cut(s) | 22 |
| Esp3I | CGTCTC | 1 cut(s) | 1617 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 127, 159 |
| FaqI | GGGAC | 3 cut(s) | 1702, 1760, 1869 |
| FbaI | TGATCA | 1 cut(s) | 46 |
| FblI | GTMKAC | 1 cut(s) | 954 |
| Fnu4HI | GCNGC | 8 cut(s) | 273, 432, 445, 762, 884, 1444, 1928, 2030 |
| FokI | GGATG | 4 cut(s) | 315, 634, 2038, 2049 |
| FriOI | GRGCYC | 2 cut(s) | 69, 1950 |
| Fsp4HI | GCNGC | 8 cut(s) | 273, 432, 445, 762, 884, 1444, 1928, 2030 |
| FspBI | CTAG | 5 cut(s) | 215, 558, 873, 1103, 1902 |
| GlaI | GCGC | 2 cut(s) | 1442, 1749 |
| GluI | GCNGC | 8 cut(s) | 273, 432, 445, 762, 884, 1444, 1928, 2030 |
| GsaI | CCCAGC | 1 cut(s) | 1920 |
| GsuI | CTGGAG | 2 cut(s) | 248, 1544 |
| HaeII | RGCGCY | 1 cut(s) | 1444 |
| HaeIII | GGCC | 6 cut(s) | 21, 264, 1101, 1325, 1769, 1876 |
| HapII | CCGG | 4 cut(s) | 943, 1576, 1795, 1942 |
| HgaI | GACGC | 1 cut(s) | 1634 |
| HhaI | GCGC | 2 cut(s) | 1443, 1750 |
| Hin1I | GRCGYC | 1 cut(s) | 1441 |
| Hin6I | GCGC | 2 cut(s) | 1441, 1748 |
| HinP1I | GCGC | 2 cut(s) | 1441, 1748 |
| HincII | GTYRAC | 2 cut(s) | 163, 1564 |
| HindII | GTYRAC | 2 cut(s) | 163, 1564 |
| HindIII | AAGCTT | 1 cut(s) | 352 |
| HpaI | GTTAAC | 1 cut(s) | 1564 |
| HpaII | CCGG | 4 cut(s) | 943, 1576, 1795, 1942 |
| HphI | GGTGA | 2 cut(s) | 164, 2030 |
| Hpy166II | GTNNAC | 8 cut(s) | 163, 346, 955, 983, 1022, 1316, 1361, 1564 |
| Hpy8I | GTNNAC | 8 cut(s) | 163, 346, 955, 983, 1022, 1316, 1361, 1564 |
| HpyAV | CCTTC | 6 cut(s) | 113, 566, 731, 1091, 1264, 2040 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 390, 420, 767, 892, 1115, 2066 |
| Hsp92I | GRCGYC | 1 cut(s) | 1441 |
| HspAI | GCGC | 2 cut(s) | 1441, 1748 |
| KasI | GGCGCC | 1 cut(s) | 1440 |
| KpnI | GGTACC | 1 cut(s) | 604 |
| Ksp22I | TGATCA | 1 cut(s) | 46 |
| KspAI | GTTAAC | 1 cut(s) | 1564 |
| Kzo9I | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| LguI | GCTCTTC | 2 cut(s) | 1739, 1757 |
| LmnI | GCTCC | 3 cut(s) | 72, 1935, 1945 |
| Lsp1109I | GCAGC | 7 cut(s) | 259, 418, 456, 748, 895, 1939, 2016 |
| LweI | GCATC | 3 cut(s) | 283, 656, 2016 |
| MabI | ACCWGGT | 1 cut(s) | 1402 |
| MaeI | CTAG | 5 cut(s) | 215, 558, 873, 1103, 1902 |
| MaeIII | GTNAC | 2 cut(s) | 416, 1711 |
| MalI | GATC | 6 cut(s) | 48, 688, 783, 907, 1129, 1556 |
| MboI | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| MfeI | CAATTG | 1 cut(s) | 1386 |
| MflI | RGATCY | 2 cut(s) | 905, 1127 |
| MhlI | GDGCHC | 4 cut(s) | 69, 192, 985, 1950 |
| Mly113I | GGCGCC | 1 cut(s) | 1441 |
| MlyI | GAGTC | 2 cut(s) | 503, 842 |
| MmeI | TCCRAC | 1 cut(s) | 228 |
| Mph1103I | ATGCAT | 1 cut(s) | 1674 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1515 |
| MseI | TTAA | 9 cut(s) | 12, 300, 857, 1181, 1563, 1580, 1634, 1644, 1653 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1743 |
| MspA1I | CMGCKG | 1 cut(s) | 761 |
| MspI | CCGG | 4 cut(s) | 943, 1576, 1795, 1942 |
| MspR9I | CCNGG | 3 cut(s) | 227, 1404, 1943 |
| MunI | CAATTG | 1 cut(s) | 1386 |
| Mva1269I | GAATGC | 1 cut(s) | 1045 |
| MvaI | CCWGG | 2 cut(s) | 227, 1404 |
| MvnI | CGCG | 1 cut(s) | 1782 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 390, 420, 767, 892, 1115, 2066 |
| NarI | GGCGCC | 1 cut(s) | 1441 |
| NciI | CCSGG | 1 cut(s) | 1943 |
| NcoI | CCATGG | 1 cut(s) | 22 |
| NdeII | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| NlaIV | GGNNCC | 3 cut(s) | 68, 602, 1442 |
| NmuCI | GTSAC | 1 cut(s) | 1711 |
| NruI | TCGCGA | 1 cut(s) | 1782 |
| NsiI | ATGCAT | 1 cut(s) | 1674 |
| NspI | RCATGY | 4 cut(s) | 247, 541, 921, 1212 |
| NspV | TTCGAA | 1 cut(s) | 1806 |
| PaeI | GCATGC | 1 cut(s) | 1212 |
| PagI | TCATGA | 2 cut(s) | 79, 844 |
| PaqCI | CACCTGC | 1 cut(s) | 1711 |
| PceI | AGGCCT | 2 cut(s) | 1101, 1876 |
| PciI | ACATGT | 1 cut(s) | 917 |
| PciSI | GCTCTTC | 2 cut(s) | 1739, 1757 |
| PctI | GAATGC | 1 cut(s) | 1045 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1515 |
| PfeI | GAWTC | 8 cut(s) | 131, 310, 524, 703, 776, 820, 827, 851 |
| PflFI | GACNNNGTC | 1 cut(s) | 1628 |
| PinAI | ACCGGT | 2 cut(s) | 1575, 1794 |
| PkrI | GCNGC | 8 cut(s) | 274, 433, 446, 763, 885, 1445, 1929, 2031 |
| PleI | GAGTC | 2 cut(s) | 502, 841 |
| PluTI | GGCGCC | 1 cut(s) | 1444 |
| PpsI | GAGTC | 2 cut(s) | 502, 841 |
| PscI | ACATGT | 1 cut(s) | 917 |
| PshBI | ATTAAT | 1 cut(s) | 1653 |
| Psp124BI | GAGCTC | 1 cut(s) | 1950 |
| Psp6I | CCWGG | 2 cut(s) | 225, 1402 |
| PspFI | CCCAGC | 1 cut(s) | 1916 |
| PspGI | CCWGG | 2 cut(s) | 225, 1402 |
| PspN4I | GGNNCC | 3 cut(s) | 68, 602, 1442 |
| PspPI | GGNCC | 3 cut(s) | 262, 1323, 1361 |
| PstI | CTGCAG | 1 cut(s) | 2034 |
| PsuI | RGATCY | 2 cut(s) | 905, 1127 |
| PsyI | GACNNNGTC | 1 cut(s) | 1628 |
| PvuII | CAGCTG | 1 cut(s) | 761 |
| RruI | TCGCGA | 1 cut(s) | 1782 |
| RsaI | GTAC | 6 cut(s) | 169, 197, 347, 602, 635, 1487 |
| RsaNI | GTAC | 6 cut(s) | 168, 196, 346, 601, 634, 1486 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1743 |
| SacI | GAGCTC | 1 cut(s) | 1950 |
| SapI | GCTCTTC | 2 cut(s) | 1739, 1757 |
| SaqAI | TTAA | 9 cut(s) | 12, 300, 857, 1181, 1563, 1580, 1634, 1644, 1653 |
| SatI | GCNGC | 8 cut(s) | 273, 432, 445, 762, 884, 1444, 1928, 2030 |
| Sau3AI | GATC | 6 cut(s) | 46, 686, 781, 905, 1127, 1554 |
| Sau96I | GGNCC | 3 cut(s) | 262, 1323, 1361 |
| SchI | GAGTC | 2 cut(s) | 503, 842 |
| ScrFI | CCNGG | 3 cut(s) | 227, 1404, 1943 |
| SduI | GDGCHC | 4 cut(s) | 69, 192, 985, 1950 |
| SexAI | ACCWGGT | 1 cut(s) | 1402 |
| SfaNI | GCATC | 3 cut(s) | 283, 656, 2016 |
| SfcI | CTRYAG | 1 cut(s) | 2030 |
| SfoI | GGCGCC | 1 cut(s) | 1442 |
| SfuI | TTCGAA | 1 cut(s) | 1806 |
| SinI | GGWCC | 1 cut(s) | 1361 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1743 |
| SmlI | CTYRAG | 3 cut(s) | 191, 593, 1028 |
| SmoI | CTYRAG | 3 cut(s) | 191, 593, 1028 |
| SphI | GCATGC | 1 cut(s) | 1212 |
| SseBI | AGGCCT | 2 cut(s) | 1101, 1876 |
| SsiI | CCGC | 1 cut(s) | 1444 |
| SspDI | GGCGCC | 1 cut(s) | 1440 |
| SspI | AATATT | 2 cut(s) | 1457, 1818 |
| SspMI | CTAG | 5 cut(s) | 215, 558, 873, 1103, 1902 |
| SstI | GAGCTC | 1 cut(s) | 1950 |
| StuI | AGGCCT | 2 cut(s) | 1101, 1876 |
| StyD4I | CCNGG | 3 cut(s) | 225, 1402, 1941 |
| StyI | CCWWGG | 1 cut(s) | 22 |
| TaqI | TCGA | 4 cut(s) | 1411, 1519, 1755, 1806 |
| TatI | WGTACW | 3 cut(s) | 195, 633, 1485 |
| TauI | GCSGC | 1 cut(s) | 1446 |
| TfiI | GAWTC | 8 cut(s) | 131, 310, 524, 703, 776, 820, 827, 851 |
| Tru1I | TTAA | 9 cut(s) | 12, 300, 857, 1181, 1563, 1580, 1634, 1644, 1653 |
| Tru9I | TTAA | 9 cut(s) | 12, 300, 857, 1181, 1563, 1580, 1634, 1644, 1653 |
| TscAI | CASTG | 4 cut(s) | 611, 636, 1777, 1898 |
| TseFI | GTSAC | 1 cut(s) | 1711 |
| TseI | GCWGC | 7 cut(s) | 272, 431, 444, 761, 883, 1927, 2029 |
| Tsp45I | GTSAC | 1 cut(s) | 1711 |
| TspGWI | ACGGA | 2 cut(s) | 640, 1841 |
| TspRI | CASTG | 4 cut(s) | 611, 636, 1777, 1898 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1628 |
| VneI | GTGCAC | 1 cut(s) | 981 |
| VpaK11BI | GGWCC | 1 cut(s) | 1361 |
| VspI | ATTAAT | 1 cut(s) | 1653 |
| XapI | RAATTY | 6 cut(s) | 31, 553, 640, 1175, 1587, 1636 |
| XbaI | TCTAGA | 2 cut(s) | 214, 557 |
| XceI | RCATGY | 4 cut(s) | 247, 541, 921, 1212 |
| XmiI | GTMKAC | 1 cut(s) | 954 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1515 |
| XspI | CTAG | 5 cut(s) | 215, 558, 873, 1103, 1902 |
| Zsp2I | ATGCAT | 1 cut(s) | 1674 |